RPL24

Summary

RPL24 (ribosomal protein L24, HGNC:10325) is a protein-coding gene on chromosome 3q12.3, encoding Large ribosomal subunit protein eL24 (P83731). Component of the large ribosomal subunit.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Source: NCBI Gene 6152 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 16 total
• Druggable target: yes — 1 molecules with ChEMBL bioactivity
• MANE Select transcript: NM_000986

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:10325
Approved symbol	RPL24
Name	ribosomal protein L24
Location	3q12.3
Locus type	gene with protein product
Status	Approved
Aliases	L24, eL24
Ensembl gene	ENSG00000114391
Ensembl biotype	protein_coding
OMIM	604180
Entrez	6152

Gene structure

Transcript identifiers

Ensembl transcripts: 18 — 10 protein_coding, 5 retained_intron, 2 protein_coding_CDS_not_defined, 1 nonsense_mediated_decay

ENST00000394077, ENST00000464595, ENST00000469605, ENST00000470961, ENST00000488288, ENST00000495401, ENST00000704284, ENST00000704285, ENST00000704286, ENST00000704287, ENST00000704288, ENST00000889763, ENST00000915208, ENST00000915209, ENST00000915210, ENST00000915211, ENST00000915212, ENST00000915213

RefSeq mRNA: 1 — MANE Select: NM_000986 NM_000986

CCDS: CCDS33809

Canonical transcript exons

ENST00000394077 — 6 exons

Exon	Start	End
ENSE00001077803	101686482	101686557
ENSE00001855871	101686672	101686718
ENSE00003587860	101685818	101685928
ENSE00003604056	101682771	101682907
ENSE00003993782	101681091	101681215
ENSE00003993783	101682429	101682492

Expression profiles

Bgee: expression breadth ubiquitous, 135 present calls, max score 99.90.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 180.2790 / max 1525.7708, expressed in 1824 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
43555	180.2790	1824

Top tissues by expression

135 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
calcaneal tendon	UBERON:0003701	99.90	gold quality
pituitary gland	UBERON:0000007	99.86	gold quality
adenohypophysis	UBERON:0002196	99.86	gold quality
cortical plate	UBERON:0005343	99.86	gold quality
ganglionic eminence	UBERON:0004023	99.85	gold quality
left ovary	UBERON:0002119	99.84	gold quality
islet of Langerhans	UBERON:0000006	99.83	gold quality
ovary	UBERON:0000992	99.83	gold quality
thyroid gland	UBERON:0002046	99.83	gold quality
corpus callosum	UBERON:0002336	99.83	gold quality
endocervix	UBERON:0000458	99.82	gold quality
left lobe of thyroid gland	UBERON:0001120	99.82	gold quality
cerebellar cortex	UBERON:0002129	99.82	gold quality
cerebellar hemisphere	UBERON:0002245	99.82	gold quality
right lobe of thyroid gland	UBERON:0001119	99.81	gold quality
cerebellum	UBERON:0002037	99.81	gold quality
right ovary	UBERON:0002118	99.81	gold quality
right hemisphere of cerebellum	UBERON:0014890	99.81	gold quality
ventricular zone	UBERON:0003053	99.80	gold quality
body of uterus	UBERON:0009853	99.80	gold quality
fallopian tube	UBERON:0003889	99.79	gold quality
uterine cervix	UBERON:0000002	99.78	gold quality
myometrium	UBERON:0001296	99.78	gold quality
right uterine tube	UBERON:0001302	99.78	gold quality
nucleus accumbens	UBERON:0001882	99.78	gold quality
ectocervix	UBERON:0012249	99.78	gold quality
monocyte	CL:0000576	99.77	gold quality
leukocyte	CL:0000738	99.77	gold quality
pancreas	UBERON:0001264	99.77	gold quality
prostate gland	UBERON:0002367	99.77	gold quality

Single-cell (SCXA)

Detected in 21 experiment(s), a significant marker in 10.

Experiment	Marker?	Max mean expression
E-CURD-122	yes	94.41
E-CURD-88	yes	56.51
E-MTAB-9221	yes	55.02
E-MTAB-9067	yes	25.54
E-HCAD-13	yes	24.84
E-MTAB-10042	yes	15.33
E-CURD-112	yes	6.33
E-MTAB-9801	yes	5.63
E-HCAD-35	yes	4.63
E-MTAB-10662	no	6302.35
E-MTAB-8559	no	3871.92
E-MTAB-10596	no	3249.55
E-MTAB-8060	no	3015.27
E-MTAB-9467	no	71.43
E-CURD-46	no	44.04

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 3)

• Data suggest that ribosomal subunit protein 24 (RPL24) lysine K27 acetylation may play a role in ribosome assembly. (PMID:24970821)
• Ribosomal protein L24 overexpression in hepatocellular carcinoma. (PMID:25520117)
• Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner. (PMID:38261097)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	rpl24	ENSDARG00000099104
rattus_norvegicus	Rpl24l1	ENSRNOG00000023733
drosophila_melanogaster	RpL24	FBGN0032518
caenorhabditis_elegans	rpl-24.1	WBGENE00004436

Paralogs (1): RSL24D1 (ENSG00000137876)

Protein

Protein identifiers

Large ribosomal subunit protein eL24 — P83731 (reviewed: P83731)

Alternative names: 60S ribosomal protein L24, 60S ribosomal protein L30

All UniProt accessions (5): A0A994J4A5, C9JNW5, C9JXB8, P83731, V9HW01

UniProt curated annotations — full annotation on UniProt →

Function. Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Subunit / interactions. Component of the large ribosomal subunit.

Subcellular location. Cytoplasm.

Post-translational modifications. Mono-ADP-ribosylation at Glu-4 by PARP16 inhibits polysome assembly and mRNA loading, thereby inhibiting protein translation.

Similarity. Belongs to the eukaryotic ribosomal protein eL24 family.

RefSeq proteins (1): NP_000977* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000988	Ribosomal_eL24-rel_N	Domain
IPR011017	TRASH_dom	Domain
IPR023442	Ribosomal_eL24_CS	Conserved_site
IPR038630	L24e/L24_sf	Homologous_superfamily
IPR056366	Ribosomal_eL24	Family

Pfam: PF01246

UniProt features (17 total): modified residue 8, cross-link 4, compositionally biased region 2, chain 1, region of interest 1, mutagenesis site 1

Structure

Experimental structures (PDB)

110 structures, top 30 by resolution.

PDB	Method	Resolution (Å)
8A3D	ELECTRON MICROSCOPY	1.67
8GLP	ELECTRON MICROSCOPY	1.67
8QYX	ELECTRON MICROSCOPY	1.78
8QOI	ELECTRON MICROSCOPY	1.9
9O3W	ELECTRON MICROSCOPY	1.9
8YOO	ELECTRON MICROSCOPY	2
9C3H	ELECTRON MICROSCOPY	2
9I2D	ELECTRON MICROSCOPY	2.19
8QFD	ELECTRON MICROSCOPY	2.2
8YOP	ELECTRON MICROSCOPY	2.2
9GUL	ELECTRON MICROSCOPY	2.2
9O3V	ELECTRON MICROSCOPY	2.2
9O3Y	ELECTRON MICROSCOPY	2.2
8JDK	ELECTRON MICROSCOPY	2.26
8G5Y	ELECTRON MICROSCOPY	2.29
9S3D	ELECTRON MICROSCOPY	2.32
9RPV	ELECTRON MICROSCOPY	2.35
9S3B	ELECTRON MICROSCOPY	2.38
7OW7	ELECTRON MICROSCOPY	2.4
8K2C	ELECTRON MICROSCOPY	2.4
8XSX	ELECTRON MICROSCOPY	2.4
9SPF	ELECTRON MICROSCOPY	2.4
9SPI	ELECTRON MICROSCOPY	2.4
8JDL	ELECTRON MICROSCOPY	2.42
9S3C	ELECTRON MICROSCOPY	2.42
9QLO	ELECTRON MICROSCOPY	2.47
7XNY	ELECTRON MICROSCOPY	2.5
8JDJ	ELECTRON MICROSCOPY	2.5
9RUC	ELECTRON MICROSCOPY	2.5
8G60	ELECTRON MICROSCOPY	2.54

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P83731-F1	81.19	0.48

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (12): 131, 149, 2, 27, 35, 147, 4, 27, 77, 83, 86, 93

Mutagenesis-validated functional residues (1):

Position	Phenotype
4	abolished mono-adp-ribosylation by parp16, leading to increased protein synthesis.

Function

Pathways and Gene Ontology

Reactome pathways

16 pathways

ID	Pathway
R-HSA-156827	L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902	Peptide chain elongation
R-HSA-1799339	SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823	Viral mRNA Translation
R-HSA-2408557	Selenocysteine synthesis
R-HSA-6791226	Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72689	Formation of a pool of free 40S subunits
R-HSA-72706	GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764	Eukaryotic Translation Termination
R-HSA-9010553	Regulation of expression of SLITs and ROBOs
R-HSA-9633012	Response of EIF2AK4 (GCN2) to amino acid deficiency
R-HSA-975956	Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957	Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
R-HSA-9954709	Ribosome Quality Control (RQC) complex extracts and degrades nascent peptide
R-HSA-9954714	PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA
R-HSA-9954716	ZNF598 and the Ribosome-associated Quality Trigger (RQT) complex dissociate a ribosome stalled on a no-go mRNA

MSigDB gene sets: 284 (showing top): GOBP_CYTOPLASMIC_TRANSLATION, GOBP_RIBOSOME_BIOGENESIS, MODULE_151, GOBP_RETINAL_GANGLION_CELL_AXON_GUIDANCE, MORF_UBE2I, GOBP_CELL_CYCLE_PHASE_TRANSITION, GOBP_STRIATED_MUSCLE_CELL_DIFFERENTIATION, HSIAO_HOUSEKEEPING_GENES, GOBP_RIBOSOME_ASSEMBLY, GOBP_NEUROGENESIS, GOBP_MALE_GAMETE_GENERATION, GOBP_CRANIAL_NERVE_DEVELOPMENT, GOBP_NEGATIVE_REGULATION_OF_CELLULAR_COMPONENT_ORGANIZATION, GOBP_TRANSLATION, GOBP_ORGANELLE_FISSION

GO Biological Process (7): ribosomal large subunit assembly (GO:0000027), cytoplasmic translation (GO:0002181), translation (GO:0006412), exit from mitosis (GO:0010458), optic nerve development (GO:0021554), retinal ganglion cell axon guidance (GO:0031290), retina development in camera-type eye (GO:0060041)

GO Molecular Function (5): RNA binding (GO:0003723), mRNA binding (GO:0003729), structural constituent of ribosome (GO:0003735), cadherin binding (GO:0045296), protein binding (GO:0005515)

GO Cellular Component (10): cytoplasm (GO:0005737), endoplasmic reticulum (GO:0005783), cytosol (GO:0005829), membrane (GO:0016020), cytosolic large ribosomal subunit (GO:0022625), cytosolic ribosome (GO:0022626), synapse (GO:0045202), extracellular exosome (GO:0070062), ribosome (GO:0005840), ribonucleoprotein complex (GO:1990904)

Reactome top-level categories

Rollup of top-11 pathways:

Category	Pathways
Ribosome-associated quality control	3
Translation	2
Cap-dependent Translation Initiation	2
Nonsense-Mediated Decay (NMD)	2
Eukaryotic Translation Initiation	1
Eukaryotic Translation Elongation	1
Influenza Viral RNA Transcription and Replication	1
Selenoamino acid metabolism	1
rRNA processing in the nucleus and cytosol	1
Signaling by ROBO receptors	1
Cellular response to starvation	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
ribosome	2
cytoplasm	2
protein-RNA complex assembly	1
ribosome assembly	1
ribosomal large subunit biogenesis	1
translation	1
peptidyltransferase activity	1
translational initiation	1
translational elongation	1
translational termination	1
macromolecule biosynthetic process	1
protein metabolic process	1
protein biosynthetic process	1
mitotic cell cycle phase transition	1
mitotic nuclear division	1
cranial nerve development	1
axon guidance	1
camera-type eye development	1
anatomical structure development	1
nucleic acid binding	1
RNA binding	1
structural molecule activity	1
cell adhesion molecule binding	1
binding	1
intracellular anatomical structure	1
endomembrane system	1
intracellular membrane-bounded organelle	1
large ribosomal subunit	1
cytosolic ribosome	1
cytosol	1
cell junction	1
extracellular vesicle	1
intracellular membraneless organelle	1
protein-containing complex	1

Protein interactions and networks

STRING

0 interactions, top by confidence (×1000):

IntAct

303 interactions, top by confidence:

A	B	Type	Score
MAP3K14	CHUK	psi-mi:“MI:0914”(association)	0.950
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
NCBP2	KPNA3	psi-mi:“MI:0914”(association)	0.640
RACK1	RPS17	psi-mi:“MI:0915”(physical association)	0.610
NOM1	RPLP0	psi-mi:“MI:0914”(association)	0.530
MAPT	KIF2A	psi-mi:“MI:0914”(association)	0.530
DEFA5	NUDT19	psi-mi:“MI:0914”(association)	0.530
NAP1L1	RPL17	psi-mi:“MI:0914”(association)	0.530
DDX6	MCRIP1	psi-mi:“MI:0914”(association)	0.510
CFTR	PLEKHG3	psi-mi:“MI:0914”(association)	0.480
PPP2R2B	DDX3X	psi-mi:“MI:0914”(association)	0.460
ESR1		psi-mi:“MI:0914”(association)	0.460
FUS	DDX3X	psi-mi:“MI:0914”(association)	0.430

BioGRID (637): RPL24 (Affinity Capture-MS), RPL24 (Affinity Capture-MS), RPL24 (Affinity Capture-MS), RPL24 (Affinity Capture-MS), RPL24 (Affinity Capture-MS), EEF1A1 (Co-fractionation), RPL10 (Co-fractionation), RPL10A (Co-fractionation), RPL10L (Co-fractionation), RPL11 (Co-fractionation), RPL12 (Co-fractionation), RPL13A (Co-fractionation), RPL15 (Co-fractionation), RPL17 (Co-fractionation), RPL18 (Co-fractionation)

ESM2 similar proteins: A1XQU3, A1XQU5, G1SE28, G1SKF7, G1SZ12, G1TXF6, O65743, O94776, P21533, P37108, P38666, P47911, P50888, P50914, P55844, P61122, P61353, P61354, P61355, P61356, P61357, P61358, P83731, P83732, Q02878, Q2YGT9, Q3T0U2, Q42347, Q4R5C7, Q4R8Z4, Q58DQ3, Q63507, Q66WF5, Q6QMZ4, Q6Y263, Q862I1, Q8BP67, Q8ISQ3, Q8JGR4, Q90YQ0

Diamond homologs: A0B603, A1RXH7, A2BK96, A3DMR8, A4FYI7, A4YCQ3, A5UJN1, A6USH5, A6UT49, A6VJU5, A9A7E7, B6YWH7, C3MRJ8, C3MY94, C3MZM3, C3N7P5, C3NFS8, C4KIV3, C5A1V7, C6A1J2, G1SE28, O22165, O26357, O29492, P14116, P54064, P61122, P61123, P61124, P83731, P83732, Q07915, Q10353, Q12Z95, Q17606, Q3SZ12, Q4JAV2, Q5JGR5, Q5RF04, Q66WF5

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
RPL24	“form complex”	“60S cytosolic large ribosomal subunit”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 203 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
TRAF6 mediated NF-kB activation	5	15.8×	6e-04
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)	19	15.4×	3e-15
SRP-dependent cotranslational protein targeting to membrane	21	14.5×	4e-16
Eukaryotic Translation Termination	17	14.1×	4e-13
Peptide chain elongation	16	14.0×	2e-12
Viral mRNA Translation	16	14.0×	2e-12
PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA	16	13.8×	2e-12
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)	20	13.5×	5e-15

GO biological processes:

GO term	Partners	Fold	FDR
positive regulation of cytoplasmic translation	5	27.4×	1e-04
cytoplasmic translation	19	19.4×	2e-16
ribosomal large subunit biogenesis	6	14.7×	5e-04
mRNA stabilization	7	14.2×	1e-04
stem cell population maintenance	6	14.0×	5e-04
translation	20	11.3×	6e-13
negative regulation of translation	10	10.8×	9e-06
rRNA processing	13	10.2×	2e-07

Disease & clinical

Clinical variants and AI predictions

ClinVar

16 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	6
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

548 predictions. Top by Δscore:

Variant	Effect	Δscore
3:101681216:C:CC	acceptor_gain	1.0000
3:101682766:CTCA:C	donor_loss	1.0000
3:101682768:CAC:C	donor_loss	1.0000
3:101682769:A:AC	donor_gain	1.0000
3:101682770:C:CT	donor_gain	1.0000
3:101682904:CTTC:C	acceptor_gain	1.0000
3:101682905:TTC:T	acceptor_gain	1.0000
3:101682906:TC:T	acceptor_gain	1.0000
3:101682907:CC:C	acceptor_gain	1.0000
3:101682907:CCT:C	acceptor_loss	1.0000
3:101682908:C:CC	acceptor_gain	1.0000
3:101682908:C:G	acceptor_loss	1.0000
3:101682911:C:CT	acceptor_gain	1.0000
3:101682912:A:T	acceptor_gain	1.0000
3:101682916:C:CT	acceptor_gain	1.0000
3:101682917:A:T	acceptor_gain	1.0000
3:101685813:CTTA:C	donor_loss	1.0000
3:101685815:TACCG:T	donor_gain	1.0000
3:101685816:A:AC	donor_gain	1.0000
3:101685816:AC:A	donor_gain	1.0000
3:101685816:ACCGA:A	donor_gain	1.0000
3:101685817:C:CT	donor_gain	1.0000
3:101685817:CC:C	donor_gain	1.0000
3:101685817:CCG:C	donor_gain	1.0000
3:101685817:CCGA:C	donor_gain	1.0000
3:101685817:CCGAC:C	donor_gain	1.0000
3:101685838:T:TA	donor_gain	1.0000
3:101685924:AAAAC:A	acceptor_gain	1.0000
3:101685925:AAAC:A	acceptor_gain	1.0000
3:101685926:AAC:A	acceptor_gain	1.0000

AlphaMissense

1018 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:101685842:T:A	R56S	1.000
3:101685842:T:G	R56S	1.000
3:101685843:C:G	R56T	1.000
3:101685855:G:A	T52I	1.000
3:101685859:A:G	W51R	1.000
3:101685859:A:T	W51R	1.000
3:101686510:C:T	G18E	1.000
3:101682778:C:G	A108P	0.999
3:101682786:C:G	R105P	0.999
3:101682797:T:A	R101S	0.999
3:101682797:T:G	R101S	0.999
3:101682798:C:G	R101T	0.999
3:101682813:T:G	Q96P	0.999
3:101682818:C:A	R94S	0.999
3:101682818:C:G	R94S	0.999
3:101682826:C:G	A92P	0.999
3:101682831:A:T	I90K	0.999
3:101682850:C:G	G84R	0.999
3:101682855:A:T	I82N	0.999
3:101682860:C:A	R80S	0.999
3:101682860:C:G	R80S	0.999
3:101682861:C:A	R80M	0.999
3:101685826:C:G	G62R	0.999
3:101685826:C:T	G62R	0.999
3:101685827:C:A	K61N	0.999
3:101685827:C:G	K61N	0.999
3:101685839:C:A	R57S	0.999
3:101685839:C:G	R57S	0.999
3:101685843:C:A	R56I	0.999
3:101685857:C:A	W51C	0.999

dbSNP variants (sampled 300 via entrez): RS1000445823 (3:101687982 T>G), RS1000733750 (3:101688259 A>G), RS1000814459 (3:101687786 T>C), RS1001099590 (3:101688178 T>C), RS1001270862 (3:101684134 C>T), RS1001626527 (3:101687385 C>T), RS1002229770 (3:101688713 G>A,T), RS1002394228 (3:101684993 T>C,G), RS1002994308 (3:101684641 G>A), RS1003109461 (3:101680706 A>C,G), RS1003397144 (3:101686270 T>C), RS1004069758 (3:101682207 A>G,T), RS1004185643 (3:101681961 T>C), RS1004290194 (3:101687533 A>G), RS1004547346 (3:101685036 C>A)

Disease associations

OMIM: gene MIM:604180 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST001565_12	Schizophrenia	7.000000e-07

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL3987582 (PROTEIN NUCLEIC-ACID COMPLEX), CHEMBL6067553 (SINGLE PROTEIN)

Molecules with ChEMBL bioactivity

1 molecules (phase ≥1), by development phase (incl. off-target/promiscuous compounds).

Molecule	Name	Phase	Patents
CHEMBL6067484	GENTAMICIN SULFATE	4

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

46 potent at pChembl≥5 of 54 total, top 45 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.52	IC50	300	nM	CHEMBL4109308
6.42	IC50	380	nM	CHEMBL4109308
6.42	IC50	380	nM	CHEMBL4574496
6.41	IC50	390	nM	CHEMBL4126894
6.39	IC50	410	nM	CHEMBL4114159
6.35	IC50	450	nM	CHEMBL4126496
6.30	IC50	500	nM	CHEMBL4574496
6.30	IC50	500	nM	CHEMBL4560206
6.16	IC50	690	nM	CHEMBL4130157
6.15	IC50	710	nM	CHEMBL4108338
6.11	IC50	780	nM	CHEMBL4114159
6.09	IC50	820	nM	CHEMBL4109308
6.07	IC50	850	nM	CHEMBL4107559
6.07	IC50	850	nM	CHEMBL4533299
6.05	IC50	900	nM	CHEMBL4126894
6.05	IC50	900	nM	CHEMBL4126496
6.04	IC50	920	nM	CHEMBL4554909
5.97	IC50	1060	nM	CHEMBL4128388
5.89	IC50	1290	nM	CHEMBL4130157
5.86	IC50	1370	nM	CHEMBL4107559
5.84	IC50	1440	nM	CHEMBL4108338
5.81	IC50	1540	nM	CHEMBL4534859
5.76	IC50	1730	nM	CHEMBL4534859
5.69	IC50	2050	nM	CHEMBL4566239
5.68	IC50	2080	nM	CHEMBL4446635
5.66	IC50	2210	nM	CHEMBL4446635
5.66	EC50	2200	nM	CHEMBL4464929
5.64	IC50	2270	nM	CHEMBL4533299
5.63	IC50	2330	nM	CHEMBL4566239
5.62	IC50	2380	nM	CHEMBL4128388
5.58	IC50	2630	nM	CHEMBL4128250
5.55	IC50	2820	nM	CHEMBL4127458
5.53	IC50	2970	nM	CHEMBL4127311
5.51	IC50	3080	nM	CHEMBL4126072
5.46	IC50	3500	nM	CHEMBL4525277
5.44	IC50	3630	nM	CHEMBL4469712
5.39	IC50	4100	nM	CHEMBL4128560
5.37	IC50	4300	nM	CHEMBL4127016
5.36	IC50	4380	nM	CHEMBL4527910
5.16	IC50	7000	nM	CHEMBL4109308
5.13	IC50	7400	nM	CHLORAMPHENICOL SULFATE SALT
5.09	IC50	8040	nM	CHEMBL4128250
5.08	IC50	8370	nM	CHEMBL4128250
5.03	IC50	9320	nM	CHEMBL4127016
5.03	IC50	9240	nM	CHEMBL4128560

PubChem BioAssay actives

46 with measured affinity, of 209 total; 26 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-4-(triazolo[4,5-b]pyridin-3-yl)benzamide	1585498: Binding affinity to 80S ribosome in human HuH7 cells expressing human C-terminal V5/6-His-tagged PCSK9 assessed as inhibition of PCSK9 secretion after 16 to 24 hrs by AlphaLISA method	ic50	0.3000	uM
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-3-[4-(triazolo[4,5-b]pyridin-3-yl)phenyl]propanamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.3800	uM
N-isoquinolin-1-yl-N-[(3R)-piperidin-3-yl]-4-(triazolo[4,5-b]pyridin-3-yl)benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.3900	uM
N-(3-chloro-2-pyridinyl)-4-(6-methyltriazolo[4,5-b]pyridin-3-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.4100	uM
N-(3-chloro-2-pyridinyl)-4-(5-methyltriazolo[4,5-b]pyridin-3-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.4500	uM
N-(1-methylpyrrolo[2,3-c]pyridin-7-yl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	0.5000	uM
N-isoquinolin-1-yl-N-[(3R)-piperidin-3-yl]-4-pyrazolo[1,5-a]pyrimidin-3-ylbenzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.6900	uM
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-5-(triazolo[4,5-b]pyridin-3-yl)pyridine-2-carboxamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.7100	uM
N-(3-chloro-2-pyridinyl)-3-[5-(6-methyltriazolo[4,5-b]pyridin-3-yl)-2-pyridinyl]-N-[(3R)-piperidin-3-yl]propanamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.8500	uM
N-(3-chloro-2-pyridinyl)-5-(6-methyltriazolo[4,5-b]pyridin-3-yl)-N-[(3R)-piperidin-3-yl]pyridine-2-carboxamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.8500	uM
N-(3-methyl-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-4-pyrazolo[1,5-a]pyrimidin-3-ylpiperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	0.9200	uM
N-isoquinolin-1-yl-4-(1-methylpyrazol-4-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	1.0600	uM
N-isoquinolin-1-yl-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	1.5400	uM
N-(3-methyl-2-pyridinyl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	2.0500	uM
N-(3-chloro-2-pyridinyl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	2.0800	uM
N-(5,8-dihydroisoquinolin-1-yl)-3-(4-methoxyphenyl)-N-[(3R)-piperidin-3-yl]propanamide	1584424: Inhibition of human 80S ribosome-mediated PCSK9 translation expressed in CHO-K1 cells assessed as reduction in PCSK9 secretion	ec50	2.2000	uM
N-isoquinolin-1-yl-3-(4-methoxyphenyl)-N-[(3R)-piperidin-3-yl]propanamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	2.6300	uM
N-(3-chloro-2-pyridinyl)-4-(6-methylpyrazin-2-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497963: Binding affinity to 80S ribosome in human HuH7 cells assessed as inhibition of PCSK9 mRNA translation after overnight incubation by ELISA	ic50	2.8200	uM
N-(3-chloro-2-pyridinyl)-4-[6-(dimethylamino)pyrazin-2-yl]-N-[(3R)-piperidin-3-yl]benzamide	1497963: Binding affinity to 80S ribosome in human HuH7 cells assessed as inhibition of PCSK9 mRNA translation after overnight incubation by ELISA	ic50	2.9700	uM
N-(3-chloro-2-pyridinyl)-4-[6-(methylamino)pyrazin-2-yl]-N-[(3R)-piperidin-3-yl]benzamide	1497963: Binding affinity to 80S ribosome in human HuH7 cells assessed as inhibition of PCSK9 mRNA translation after overnight incubation by ELISA	ic50	3.0800	uM
N-isoquinolin-1-yl-4-(6-methyl-1,2-benzoxazol-3-yl)-N-[(3R)-piperidin-3-yl]piperazine-1-carboxamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	3.5000	uM
N-(3-methylpyrazin-2-yl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	3.6300	uM
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-6-(triazolo[4,5-b]pyridin-3-yl)pyridine-3-carboxamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	4.1000	uM
N-isoquinolin-1-yl-N-[(3R)-piperidin-3-yl]-4-pyrazin-2-ylbenzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	4.3000	uM
4-(2-fluorophenyl)-N-(3-methyl-2-pyridinyl)-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	4.3800	uM
2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide;sulfuric acid	717551: Inhibition of mitochondrial ribosome-mediated protein synthesis in human HeLa cells assessed as {35S]methionine incorporation by autoradiography	ic50	7.4000	uM

CTD chemical–gene interactions

63 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects expression, decreases expression	4
sodium arsenite	decreases expression, increases abundance, increases expression, affects binding, decreases reaction	4
Air Pollutants	decreases expression, increases abundance	2
Smoke	decreases expression, increases abundance	2
Cadmium Chloride	increases abundance, increases expression	2
bisphenol F	increases expression	1
TAK-243	increases sumoylation	1
2,4,6-tribromophenol	decreases expression	1
uranyl acetate	affects expression	1
butylphen	increases expression	1
deoxynivalenol	increases expression	1
pyrogallol 1,3-dimethyl ether	affects cotreatment, affects localization, increases expression	1
decabromobiphenyl ether	decreases expression	1
arsenite	affects binding, increases reaction	1
tetrabromobisphenol A	decreases expression	1
perfluorooctanoic acid	increases expression	1
phenanthrene	decreases expression	1
cyclic 3’,5’-uridine monophosphate	affects binding	1
CD 437	decreases expression	1
chloropicrin	decreases expression	1
perfluoro-n-nonanoic acid	decreases expression	1
corosolic acid	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
3-(4’-hydroxy-3’-adamantylbiphenyl-4-yl)acrylic acid	decreases expression	1
nutlin 3	affects cotreatment, increases secretion	1
bisphenol B	increases expression	1
abrine	decreases expression	1
2-amino-14,16-dimethyloctadecan-3-ol	decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
Grape Seed Proanthocyanidins	affects cotreatment, decreases expression	1

ChEMBL screening assays

90 unique, capped per target: 90 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL1920845	Binding	Induction of ribosome to readthrough in human A-T lymphoblastoid cells assessed as ATM ser1981 autophosphorylation at 30 uM after 4 days by PTT-ELISA assay	Synthesis and evaluation of compounds that induce readthrough of premature termination codons. — Bioorg Med Chem Lett

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.