RPS27

Summary

RPS27 (ribosomal protein S27, HGNC:10416) is a protein-coding gene on chromosome 1q21.3, encoding Small ribosomal subunit protein eS27 (P42677). Component of the small ribosomal subunit.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Source: NCBI Gene 6232 — RefSeq curated summary.

At a glance

• Gene–disease (curated): Diamond-Blackfan anemia 17 (Limited, GenCC)
• GWAS associations: 12
• Clinical variants (ClinVar): 13 total
• Phenotypes (HPO): 62
• Druggable target: yes — 1 molecules with ChEMBL bioactivity
• MANE Select transcript: NM_001030

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:10416
Approved symbol	RPS27
Name	ribosomal protein S27
Location	1q21.3
Locus type	gene with protein product
Status	Approved
Aliases	MPS-1, MPS1, S27, eS27
Ensembl gene	ENSG00000177954
Ensembl biotype	protein_coding
OMIM	603702
Entrez	6232

Gene structure

Transcript identifiers

Ensembl transcripts: 9 — 5 protein_coding, 3 retained_intron, 1 nonsense_mediated_decay

ENST00000392558, ENST00000477151, ENST00000493224, ENST00000643794, ENST00000651669, ENST00000936803, ENST00000936804, ENST00000936805, ENST00000936806

RefSeq mRNA: 3 — MANE Select: NM_001030 NM_001030, NM_001349946, NM_001349947

CCDS: CCDS1059

Canonical transcript exons

ENST00000651669 — 4 exons

Exon	Start	End
ENSE00003468875	153991566	153991676
ENSE00003669489	153992065	153992155
ENSE00003688128	153991115	153991223
ENSE00003844361	153990762	153990802

Expression profiles

Bgee: expression breadth ubiquitous, 134 present calls, max score 99.99.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 822.6043 / max 9213.7604, expressed in 1827 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
5463	821.9658	1827
5464	0.6384	337

Top tissues by expression

134 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
ganglionic eminence	UBERON:0004023	99.99	gold quality
granulocyte	CL:0000094	99.98	gold quality
lymph node	UBERON:0000029	99.98	gold quality
endocervix	UBERON:0000458	99.98	gold quality
right ovary	UBERON:0002118	99.98	gold quality
left ovary	UBERON:0002119	99.98	gold quality
ovary	UBERON:0000992	99.97	gold quality
corpus callosum	UBERON:0002336	99.97	gold quality
ventricular zone	UBERON:0003053	99.97	gold quality
calcaneal tendon	UBERON:0003701	99.97	gold quality
fallopian tube	UBERON:0003889	99.97	gold quality
cortical plate	UBERON:0005343	99.97	gold quality
body of uterus	UBERON:0009853	99.97	gold quality
ectocervix	UBERON:0012249	99.97	gold quality
pituitary gland	UBERON:0000007	99.96	gold quality
zone of skin	UBERON:0000014	99.96	gold quality
vagina	UBERON:0000996	99.96	gold quality
adipose tissue	UBERON:0001013	99.96	gold quality
right lobe of thyroid gland	UBERON:0001119	99.96	gold quality
left lobe of thyroid gland	UBERON:0001120	99.96	gold quality
mucosa of stomach	UBERON:0001199	99.96	gold quality
right uterine tube	UBERON:0001302	99.96	gold quality
left uterine tube	UBERON:0001303	99.96	gold quality
skin of abdomen	UBERON:0001416	99.96	gold quality
skin of leg	UBERON:0001511	99.96	gold quality
thyroid gland	UBERON:0002046	99.96	gold quality
spleen	UBERON:0002106	99.96	gold quality
subcutaneous adipose tissue	UBERON:0002190	99.96	gold quality
thoracic mammary gland	UBERON:0005200	99.96	gold quality
myometrium	UBERON:0001296	99.95	gold quality

Single-cell (SCXA)

Detected in 70 experiment(s), a significant marker in 21.

Experiment	Marker?	Max mean expression
E-MTAB-6701	yes	20879.66
E-MTAB-7407	yes	18085.64
E-CURD-88	yes	17943.85
E-CURD-46	yes	17717.83
E-HCAD-1	yes	17324.87
E-MTAB-9067	yes	17281.51
E-HCAD-9	yes	16945.99
E-MTAB-8410	yes	16161.81
E-MTAB-9221	yes	15971.65
E-CURD-122	yes	15331.42
E-GEOD-135922	yes	12764.27
E-CURD-98	yes	7886.73
E-MTAB-10042	yes	7379.03
E-MTAB-7316	yes	6700.47
E-MTAB-10855	yes	4958.33

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 16)

• MPS-1 was overexpressed in 86% of the gastric cancer tissues and all gastric cancer cells. MPS-1 expression was significantly increased and corresponded with the tumor-node-metastasis clinical stage, and was significantly higher in the late stage. (PMID:16914586)
• When MPS-1 is overexpressed, it has an extraribosomal function as a strong inhibitor of HNSCC tumor cell growth, which may be exerted by reduced paxillin gene expression. (PMID:19642098)
• study reveals a multilevel interplay between RPS27L/S27 and p53-MDM2 axis, with RPS27L functioning as a p53 target, a MDM2 substrate and a p53 regulator (PMID:21170087)
• This work is consistent with a critical role of RPMPS-1/S27 in the life cycle of various viruses and shows that disruption of viral ZFPs is potentially important to control and prevent deathly viral diseases (PMID:21518817)
• a novel pathway, the MPS-1/NF-kappaB/Gadd45beta signal pathway, played an important role in MPS-1 knockdown-induced apoptosis of gastric cancer cells. (PMID:21796632)
• The data on genome context analysis indicates that the presence of MPS-1 in the blood is an indicator of oncogenesis (PMID:22798506)
• We identified frequent recurrent (i.e. hotspot) mutation in the 5’ untranslated region of RPS27 in ~10% of melanoma samples (PMID:24913145)
• Loss of function mutations in RPL27 and RPS27 lead to Diamond-Blackfan anaemia. (PMID:25424902)
• MPS1 is a new marker of Purkinje Cells. (PMID:26708598)
• reveal how Mps1 dynamically modifies kinetochores to correct improper attachments and ensure faithful chromosome segregation (PMID:28441529)
• Chloride anion behaves as a signaling effector for CFTR in the modulation of RPS27 expression. (PMID:28941802)
• this study indicates that MPS-1 promotes leptin-induced colorectal cancer (CRC) via activating JNK/c-Jun pathway. MPS-1 might represent a potent candidate for the treatment and prognostic prediction of obesity-associated CRC. (PMID:31506433)
• Functional analysis of RPS27 mutations and expression in melanoma. (PMID:31663663)
• Neddylation modification of ribosomal protein RPS27L or RPS27 by MDM2 or NEDP1 regulates cancer cell survival. (PMID:32779270)
• Hsp90 chaperone code and the tumor suppressor VHL cooperatively regulate the mitotic checkpoint. (PMID:34586601)
• RPS27 selectively regulates the expression and alternative splicing of inflammatory and immune response genes in thyroid cancer cells. (PMID:35546566)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
rattus_norvegicus		ENSRNOG00000070133

Paralogs (1): RPS27L (ENSG00000185088)

Protein

Protein identifiers

Small ribosomal subunit protein eS27 — P42677 (reviewed: P42677)

Alternative names: 40S ribosomal protein S27, Metallopan-stimulin 1

All UniProt accessions (2): A0A2R8Y731, P42677

UniProt curated annotations — full annotation on UniProt →

Function. Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for proper rRNA processing and maturation of 18S rRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

Subunit / interactions. Component of the small ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.

Subcellular location. Cytoplasm. Nucleus. Nucleolus.

Tissue specificity. Expressed in a wide variety of actively proliferating cells and tumor tissues.

Disease relevance. Diamond-Blackfan anemia 17 (DBA17) [MIM:617409] An autosomal dominant form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. The disease is caused by variants affecting the gene represented in this entry.

Cofactor. Binds 1 zinc ion per subunit.

Similarity. Belongs to the eukaryotic ribosomal protein eS27 family.

RefSeq proteins (3): NP_001021, NP_001336875, NP_001336876 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000592	Ribosomal_eS27	Family
IPR011332	Ribosomal_zn-bd	Homologous_superfamily
IPR023407	Ribosomal_eS27_Zn-bd_dom_sf	Homologous_superfamily

Pfam: PF01667

UniProt features (23 total): strand 10, binding site 4, helix 2, initiator methionine 1, chain 1, sequence conflict 1, zinc finger region 1, region of interest 1, compositionally biased region 1, modified residue 1

Structure

Experimental structures (PDB)

214 structures, top 30 by resolution.

PDB	Method	Resolution (Å)
8GLP	ELECTRON MICROSCOPY	1.67
8QOI	ELECTRON MICROSCOPY	1.9
9O3W	ELECTRON MICROSCOPY	1.9
8YOO	ELECTRON MICROSCOPY	2
9C3H	ELECTRON MICROSCOPY	2
7R4X	ELECTRON MICROSCOPY	2.15
9I2D	ELECTRON MICROSCOPY	2.19
9PBE	ELECTRON MICROSCOPY	2.19
8YOP	ELECTRON MICROSCOPY	2.2
9O3V	ELECTRON MICROSCOPY	2.2
9O3Y	ELECTRON MICROSCOPY	2.2
8JDK	ELECTRON MICROSCOPY	2.26
8G5Y	ELECTRON MICROSCOPY	2.29
9S3D	ELECTRON MICROSCOPY	2.32
9RPV	ELECTRON MICROSCOPY	2.35
9S3B	ELECTRON MICROSCOPY	2.38
8K2C	ELECTRON MICROSCOPY	2.4
8XSX	ELECTRON MICROSCOPY	2.4
9SPF	ELECTRON MICROSCOPY	2.4
9SPI	ELECTRON MICROSCOPY	2.4
8JDL	ELECTRON MICROSCOPY	2.42
9S3C	ELECTRON MICROSCOPY	2.42
9QLO	ELECTRON MICROSCOPY	2.47
9P8B	ELECTRON MICROSCOPY	2.48
7XNY	ELECTRON MICROSCOPY	2.5
8JDJ	ELECTRON MICROSCOPY	2.5
9RUC	ELECTRON MICROSCOPY	2.5
8G60	ELECTRON MICROSCOPY	2.54
8IFE	ELECTRON MICROSCOPY	2.57
9P7D	ELECTRON MICROSCOPY	2.57

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P42677-F1	92.47	0.88

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 37; 40; 56; 59

Post-translational modifications (1): 11

Function

Pathways and Gene Ontology

Reactome pathways

68 pathways

ID	Pathway
R-HSA-141444	Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-156827	L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902	Peptide chain elongation
R-HSA-1799339	SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823	Viral mRNA Translation
R-HSA-2408557	Selenocysteine synthesis
R-HSA-2467813	Separation of Sister Chromatids
R-HSA-2500257	Resolution of Sister Chromatid Cohesion
R-HSA-5663220	RHO GTPases Activate Formins
R-HSA-6791226	Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-68877	Mitotic Prometaphase
R-HSA-72649	Translation initiation complex formation
R-HSA-72689	Formation of a pool of free 40S subunits
R-HSA-72695	Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702	Ribosomal scanning and start codon recognition
R-HSA-72706	GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764	Eukaryotic Translation Termination
R-HSA-9010553	Regulation of expression of SLITs and ROBOs
R-HSA-9633012	Response of EIF2AK4 (GCN2) to amino acid deficiency
R-HSA-9648025	EML4 and NUDC in mitotic spindle formation
R-HSA-9735869	SARS-CoV-1 modulates host translation machinery
R-HSA-9754678	SARS-CoV-2 modulates host translation machinery
R-HSA-975956	Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957	Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
R-HSA-9954714	PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA
R-HSA-9954716	ZNF598 and the Ribosome-associated Quality Trigger (RQT) complex dissociate a ribosome stalled on a no-go mRNA
R-HSA-1266738	Developmental Biology
R-HSA-141424	Amplification of signal from the kinetochores
R-HSA-1430728	Metabolism
R-HSA-156842	Eukaryotic Translation Elongation

MSigDB gene sets: 454 (showing top): GOBP_CYTOPLASMIC_TRANSLATION, SHEPARD_BMYB_MORPHOLINO_UP, GOBP_RIBOSOME_BIOGENESIS, YAGI_AML_WITH_INV_16_TRANSLOCATION, MODULE_151, GNF2_TPT1, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, HSIAO_HOUSEKEEPING_GENES, GOBP_RIBOSOME_ASSEMBLY, GGCNKCCATNK_UNKNOWN, GOBP_TRANSLATION, WATANABE_RECTAL_CANCER_RADIOTHERAPY_RESPONSIVE_UP, GOBP_RIBOSOMAL_SMALL_SUBUNIT_BIOGENESIS, GOMF_STRUCTURAL_CONSTITUENT_OF_RIBOSOME, GOBP_PROTEIN_RNA_COMPLEX_ORGANIZATION

GO Biological Process (5): ribosomal small subunit assembly (GO:0000028), cytoplasmic translation (GO:0002181), rRNA processing (GO:0006364), translation (GO:0006412), ribosomal small subunit biogenesis (GO:0042274)

GO Molecular Function (6): DNA binding (GO:0003677), RNA binding (GO:0003723), structural constituent of ribosome (GO:0003735), zinc ion binding (GO:0008270), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (14): nucleus (GO:0005634), nucleoplasm (GO:0005654), cytosol (GO:0005829), ribosome (GO:0005840), postsynaptic density (GO:0014069), cytosolic ribosome (GO:0022626), cytosolic small ribosomal subunit (GO:0022627), small-subunit processome (GO:0032040), presynapse (GO:0098793), glutamatergic synapse (GO:0098978), GABA-ergic synapse (GO:0098982), nucleolus (GO:0005730), cytoplasm (GO:0005737), ribonucleoprotein complex (GO:1990904)

Reactome top-level categories

Rollup of top-15 pathways:

Category	Pathways
Cap-dependent Translation Initiation	4
Translation	2
Mitotic Prometaphase	2
Amplification of signal from the kinetochores	1
Eukaryotic Translation Initiation	1
Eukaryotic Translation Elongation	1
Influenza Viral RNA Transcription and Replication	1
Selenoamino acid metabolism	1
Mitotic Anaphase	1
RHO GTPase Effectors	1
rRNA processing in the nucleus and cytosol	1
M Phase	1
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S	1
Signaling by ROBO receptors	1
Cellular response to starvation	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	4
synapse	3
ribosome biogenesis	2
nucleic acid binding	2
ribosome	2
nuclear lumen	2
intracellular membraneless organelle	2
protein-RNA complex assembly	1
ribosome assembly	1
ribosomal small subunit biogenesis	1
translation	1
RNA processing	1
rRNA metabolic process	1
peptidyltransferase activity	1
translational initiation	1
translational elongation	1
translational termination	1
macromolecule biosynthetic process	1
protein metabolic process	1
protein biosynthetic process	1
ribonucleoprotein complex biogenesis	1
structural molecule activity	1
transition metal ion binding	1
binding	1
cation binding	1
intracellular membrane-bounded organelle	1
cytoplasm	1
asymmetric synapse	1
postsynaptic specialization	1
cytosol	1
small ribosomal subunit	1
cytosolic ribosome	1
nucleolus	1
preribosome	1
t-UTP complex	1
nuclear protein-containing complex	1
intracellular anatomical structure	1
protein-containing complex	1

Protein interactions and networks

STRING

3092 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
RPS27	RPS26	P02383	864
RPS27	RPS28	P25112	854
RPS27	RPS29	P30054	840
RPS27	RPL27	P08526	836
RPS27	MDM2	Q00987	821
RPS27	RPS19	P39019	815
RPS27	RPS25	P25111	806
RPS27	RPS14	P06366	793
RPS27	RPS15A	P39027	763
RPS27	RPS24	P16632	759
RPS27	RPL5	P46777	757
RPS27	RPL38	P23411	752
RPS27	RPL35	P42766	746
RPS27	RPL26	P61254	742
RPS27	RPL30	P04645	737

IntAct

184 interactions, top by confidence:

A	B	Type	Score
MAP3K14	CHUK	psi-mi:“MI:0914”(association)	0.950
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
NCBP2	KPNA3	psi-mi:“MI:0914”(association)	0.640
LRRK2	RPS27	psi-mi:“MI:0217”(phosphorylation reaction)	0.640
RACK1	RPS17	psi-mi:“MI:0915”(physical association)	0.610
MDM2	RPS27	psi-mi:“MI:0407”(direct interaction)	0.600
MDM2	RPS27	psi-mi:“MI:0915”(physical association)	0.600
RPS27	MDM2	psi-mi:“MI:0915”(physical association)	0.600
RPS27	PSME3	psi-mi:“MI:0915”(physical association)	0.560
ENOPH1	RPS27	psi-mi:“MI:0915”(physical association)	0.550
BLM	RPS27	psi-mi:“MI:0915”(physical association)	0.540
BLM	RPS27	psi-mi:“MI:0217”(phosphorylation reaction)	0.540
SYNGAP1	IGF2BP3	psi-mi:“MI:0914”(association)	0.530
RPS18	RPS2	psi-mi:“MI:0914”(association)	0.530
P/V	IRS4	psi-mi:“MI:0914”(association)	0.530
PPP2R2B	DDX3X	psi-mi:“MI:0914”(association)	0.460
TUBA1A	TUBAL3	psi-mi:“MI:0914”(association)	0.420
RPS27	RPS7	psi-mi:“MI:0915”(physical association)	0.400
RPS27	SKIL	psi-mi:“MI:0915”(physical association)	0.370
RPS27	ENTPD4	psi-mi:“MI:0915”(physical association)	0.370
TOM1	RPS27	psi-mi:“MI:0915”(physical association)	0.370
ACTN4	RPS27	psi-mi:“MI:0915”(physical association)	0.370

BioGRID (533): RPS27 (Affinity Capture-MS), RPS27 (Affinity Capture-MS), RPL10A (Co-fractionation), RPL12 (Co-fractionation), RPL18 (Co-fractionation), RPL18A (Co-fractionation), RPL27 (Co-fractionation), RPL27A (Co-fractionation), RPL30 (Co-fractionation), RPL6 (Co-fractionation), RPL7A (Co-fractionation), RPL8 (Co-fractionation), RPL9 (Co-fractionation), RPLP2 (Co-fractionation), RPS18 (Co-fractionation)

ESM2 similar proteins: A0A1D8PTI7, A0A1L8H579, A0A1W2PPG7, A1X8D9, G1TZ76, O64650, O74330, P03131, P06459, P09003, P0C567, P0DOC8, P11332, P17387, P24051, P35997, P36826, P36833, P38711, P40569, P42677, P47903, P47904, P55833, P68834, P68835, P68958, P68959, Q02271, Q13670, Q1X709, Q2KHT7, Q39072, Q3T0B7, Q5RBK1, Q5RGZ1, Q67616, Q67708, Q6DRC3, Q6ZWU9

Diamond homologs: A0A1D8PTI7, A1RRN0, A2BN94, A3MUV2, A4WLR8, B1YAF9, B6YT34, C5A6X9, G1TZ76, O64650, O74330, P24051, P35997, P38654, P38711, P42677, P47903, P47904, P55833, P58078, Q2KHT7, Q3T0B7, Q46C77, Q4J9B1, Q5JE50, Q5RBK1, Q6ZWU9, Q6ZWY3, Q71TY3, Q71UM5, Q7RVN2, Q8L953, Q8T1V4, Q8U474, Q8ZTY4, Q96564, Q973F9, Q97Z80, Q9M2F1, Q9TXP0

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
RPS27	“form complex”	“40S cytosolic small ribosomal subunit”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 190 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Eukaryotic Translation Initiation	7	16.5×	4e-06
Cap-dependent Translation Initiation	7	16.5×	4e-06
SARS-CoV-1 modulates host translation machinery	7	16.5×	4e-06
Formation of the ternary complex, and subsequently, the 43S complex	10	16.4×	2e-08
Nonsense-Mediated Decay (NMD)	9	16.0×	1e-07
Ribosomal scanning and start codon recognition	11	16.0×	5e-09
TNFR1-induced NF-kappa-B signaling pathway	6	15.4×	4e-05
Eukaryotic Translation Elongation	7	14.9×	8e-06

GO biological processes:

GO term	Partners	Fold	FDR
non-canonical NF-kappaB signal transduction	5	26.5×	3e-04
cytoplasmic translation	15	17.5×	7e-12
translational initiation	7	15.8×	1e-04
regulation of translational initiation	5	14.7×	3e-03
extrinsic apoptotic signaling pathway via death domain receptors	5	12.6×	5e-03
canonical NF-kappaB signal transduction	5	11.5×	6e-03
translation	17	11.0×	1e-10
tumor necrosis factor-mediated signaling pathway	5	10.4×	9e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

13 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	2
Likely benign	5
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

236 predictions. Top by Δscore:

Variant	Effect	Δscore
1:153990974:GAGA:G	donor_gain	1.0000
1:153991112:TAGCT:T	acceptor_loss	1.0000
1:153991113:A:AG	acceptor_gain	1.0000
1:153991113:A:AT	acceptor_loss	1.0000
1:153991114:G:GA	acceptor_gain	1.0000
1:153991114:GCTC:G	acceptor_gain	1.0000
1:153991220:CCAGG:C	donor_loss	1.0000
1:153991223:GGT:G	donor_loss	1.0000
1:153991224:G:GA	donor_loss	1.0000
1:153991225:T:A	donor_loss	1.0000
1:153991672:AGAAG:A	donor_loss	1.0000
1:153991673:GAAG:G	donor_gain	1.0000
1:153991673:GAAGG:G	donor_loss	1.0000
1:153991674:AAG:A	donor_loss	1.0000
1:153991675:AG:A	donor_loss	1.0000
1:153991676:GGTAA:G	donor_loss	1.0000
1:153991677:GT:G	donor_loss	1.0000
1:153991678:T:G	donor_loss	1.0000
1:153992064:GGAT:G	acceptor_gain	1.0000
1:153990799:GCCT:G	donor_gain	0.9900
1:153990803:G:GG	donor_gain	0.9900
1:153990977:A:G	donor_gain	0.9900
1:153990994:C:G	donor_gain	0.9900
1:153991010:G:T	donor_gain	0.9900
1:153991022:C:T	donor_gain	0.9900
1:153991113:AGCTC:A	acceptor_gain	0.9900
1:153991114:GC:G	acceptor_gain	0.9900
1:153991114:GCT:G	acceptor_gain	0.9900
1:153991114:GCTCG:G	acceptor_gain	0.9900
1:153991208:G:GT	donor_gain	0.9900

AlphaMissense

549 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:153991202:T:C	F32L	1.000
1:153991204:C:A	F32L	1.000
1:153991204:C:G	F32L	1.000
1:153991589:T:C	F47L	1.000
1:153991591:T:A	F47L	1.000
1:153991591:T:G	F47L	1.000
1:153991593:G:T	S48I	1.000
1:153991642:C:G	C64W	1.000
1:153992077:G:T	R80M	1.000
1:153991208:G:C	D34H	0.999
1:153991212:T:A	V35E	0.999
1:153991217:T:C	C37R	0.999
1:153991587:T:A	V46D	0.999
1:153991589:T:A	F47I	0.999
1:153991592:A:C	S48R	0.999
1:153991594:C:A	S48R	0.999
1:153991594:C:G	S48R	0.999
1:153991616:T:C	C56R	0.999
1:153991617:G:A	C56Y	0.999
1:153991618:T:G	C56W	0.999
1:153991625:T:C	C59R	0.999
1:153991626:G:A	C59Y	0.999
1:153991627:C:G	C59W	0.999
1:153991638:T:A	L63H	0.999
1:153991640:T:C	C64R	0.999
1:153991641:G:A	C64Y	0.999
1:153991653:G:A	G68E	0.999
1:153991653:G:T	G68V	0.999
1:153991656:G:A	G69E	0.999
1:153991656:G:T	G69V	0.999

dbSNP variants (sampled 300 via entrez): RS1000118647 (1:153989524 C>T), RS1000509046 (1:153988937 T>C), RS1002259443 (1:153991943 C>G), RS1002934205 (1:153989537 C>A,G), RS1004220318 (1:153989566 G>A), RS1004996538 (1:153989901 A>G), RS1005026391 (1:153990217 C>T), RS1006248863 (1:153992454 T>TC), RS1006565022 (1:153991672 A>G,T), RS1006826721 (1:153991032 C>G,T), RS1006940689 (1:153991401 A>G), RS1008501466 (1:153990078 GTTTATTTA>G,GTTTA,GTTTATTTATTTA), RS1008602878 (1:153988838 C>T), RS1009926139 (1:153989175 G>A,C,T), RS1011570090 (1:153990290 T>C)

Disease associations

OMIM: gene MIM:603702 | disease phenotypes:

GenCC curated gene-disease

Disease	Classification	Inheritance
Diamond-Blackfan anemia 17	Limited	Unknown

Mondo (1): Diamond-Blackfan anemia 17 (MONDO:0044310)

Orphanet (0):

HPO phenotypes

62 total (30 of 62 shown, HPO-id order):

HPO	Term
HP:0000006	Autosomal dominant inheritance
HP:0000047	Hypospadias
HP:0000085	Horseshoe kidney
HP:0000104	Renal agenesis
HP:0000119	Abnormality of the genitourinary system
HP:0000185	Cleft soft palate
HP:0000218	High palate
HP:0000234	Abnormality of the head
HP:0000252	Microcephaly
HP:0000286	Epicanthus
HP:0000294	Low anterior hairline
HP:0000316	Hypertelorism
HP:0000347	Micrognathia
HP:0000369	Low-set ears
HP:0000431	Wide nasal bridge
HP:0000465	Webbed neck
HP:0000470	Short neck
HP:0000486	Strabismus
HP:0000508	Ptosis
HP:0000519	Developmental cataract
HP:0000912	Sprengel anomaly
HP:0000953	Hyperpigmentation of the skin
HP:0000980	Pallor
HP:0001087	Developmental glaucoma
HP:0001199	Triphalangeal thumb
HP:0001227	Abnormality of the thenar eminence
HP:0001254	Lethargy
HP:0001510	Growth delay
HP:0001518	Small for gestational age
HP:0001627	Abnormal heart morphology

GWAS associations

12 associations (top):

Study	Trait	p-value
GCST010136_18	Fruit consumption	3.000000e-08
GCST010137_3	Cooked vegetable consumption	3.000000e-09
GCST010142_60	Fish- and plant-related diet	4.000000e-09
GCST010142_92	Fish- and plant-related diet	6.000000e-14
GCST010696_22	Cortical thickness (min-P)	4.000000e-10
GCST010697_50	Cortical surface area (min-P)	1.000000e-12
GCST010698_81	Subcortical volume (min-P)	1.000000e-23
GCST010699_7	Brain morphology (min-P)	1.000000e-10
GCST010700_11	Cortical thickness (MOSTest)	4.000000e-13
GCST010701_73	Cortical surface area (MOSTest)	4.000000e-09
GCST010702_45	Subcortical volume (MOSTest)	4.000000e-10
GCST010703_276	Brain morphology (MOSTest)	2.000000e-15

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0008111	diet measurement
EFO:0004346	neuroimaging measurement
EFO:0004840	cortical thickness

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL1932893 (SINGLE PROTEIN), CHEMBL3987582 (PROTEIN NUCLEIC-ACID COMPLEX)

Molecules with ChEMBL bioactivity

1 molecules (phase ≥1), by development phase (incl. off-target/promiscuous compounds).

Molecule	Name	Phase	Patents
CHEMBL6067484	GENTAMICIN SULFATE	4

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

59 potent at pChembl≥5 of 63 total, top 50 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.19	IC50	6.4	nM	CHEMBL2089255
7.43	IC50	37	nM	CHEMBL2089254
6.88	IC50	131	nM	CHEMBL2089255
6.64	IC50	228	nM	CHEMBL2089253
6.52	IC50	300	nM	CHEMBL4109308
6.42	IC50	380	nM	CHEMBL4109308
6.42	IC50	380	nM	CHEMBL4574496
6.41	IC50	390	nM	CHEMBL4126894
6.39	IC50	410	nM	CHEMBL4114159
6.35	IC50	450	nM	CHEMBL4126496
6.33	Kd	469.7	nM	CHEMBL5653589
6.33	ED50	469.7	nM	CHEMBL5653589
6.30	IC50	500	nM	CHEMBL4574496
6.30	IC50	500	nM	CHEMBL4560206
6.28	IC50	529	nM	CHEMBL211195
6.25	IC50	568	nM	CHEMBL424872
6.16	IC50	690	nM	CHEMBL4130157
6.15	IC50	710	nM	CHEMBL4108338
6.11	IC50	780	nM	CHEMBL4114159
6.09	IC50	820	nM	CHEMBL4109308
6.07	IC50	850	nM	CHEMBL4107559
6.07	IC50	850	nM	CHEMBL4533299
6.05	IC50	900	nM	CHEMBL4126894
6.05	IC50	900	nM	CHEMBL4126496
6.04	IC50	920	nM	CHEMBL4554909
5.97	IC50	1060	nM	CHEMBL4128388
5.89	IC50	1290	nM	CHEMBL4130157
5.86	IC50	1370	nM	CHEMBL4107559
5.84	IC50	1440	nM	CHEMBL4108338
5.81	IC50	1540	nM	CHEMBL4534859
5.76	IC50	1730	nM	CHEMBL4534859
5.75	IC50	1800	nM	CHEMBL2089254
5.69	IC50	2050	nM	CHEMBL4566239
5.68	IC50	2080	nM	CHEMBL4446635
5.66	IC50	2210	nM	CHEMBL4446635
5.66	EC50	2200	nM	CHEMBL4464929
5.64	IC50	2270	nM	CHEMBL4533299
5.63	IC50	2330	nM	CHEMBL4566239
5.62	IC50	2380	nM	CHEMBL4128388
5.58	IC50	2630	nM	CHEMBL4128250
5.55	IC50	2820	nM	CHEMBL4127458
5.53	IC50	2970	nM	CHEMBL4127311
5.51	IC50	3080	nM	CHEMBL4126072
5.46	IC50	3500	nM	CHEMBL4525277
5.44	IC50	3630	nM	CHEMBL4469712
5.40	IC50	3970	nM	CHEMBL2089252
5.39	IC50	4100	nM	CHEMBL4128560
5.38	IC50	4200	nM	CHEMBL210861
5.37	IC50	4300	nM	CHEMBL4127016
5.36	IC50	4380	nM	CHEMBL4527910

PubChem BioAssay actives

57 with measured affinity, of 229 total; 35 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-[[4-amino-6-(tert-butylamino)-5-cyano-2-pyridinyl]amino]benzamide	683374: Inhibition of Mps1-mediated p38 MAPK phosphorylation after 90 mins by DELFIA assay	ic50	0.0064	uM
4-[(4-amino-5-cyano-6-ethoxy-2-pyridinyl)amino]benzamide	683374: Inhibition of Mps1-mediated p38 MAPK phosphorylation after 90 mins by DELFIA assay	ic50	0.0370	uM
4-amino-6-anilino-2-ethoxypyridine-3-carbonitrile	683374: Inhibition of Mps1-mediated p38 MAPK phosphorylation after 90 mins by DELFIA assay	ic50	0.2280	uM
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-4-(triazolo[4,5-b]pyridin-3-yl)benzamide	1585498: Binding affinity to 80S ribosome in human HuH7 cells expressing human C-terminal V5/6-His-tagged PCSK9 assessed as inhibition of PCSK9 secretion after 16 to 24 hrs by AlphaLISA method	ic50	0.3000	uM
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-3-[4-(triazolo[4,5-b]pyridin-3-yl)phenyl]propanamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.3800	uM
N-isoquinolin-1-yl-N-[(3R)-piperidin-3-yl]-4-(triazolo[4,5-b]pyridin-3-yl)benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.3900	uM
N-(3-chloro-2-pyridinyl)-4-(6-methyltriazolo[4,5-b]pyridin-3-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.4100	uM
N-(3-chloro-2-pyridinyl)-4-(5-methyltriazolo[4,5-b]pyridin-3-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.4500	uM
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149300: Binding affinity to human RPS27 incubated for 45 mins by Kinobead based pull down assay	kd	0.4697	uM
N-(1-methylpyrrolo[2,3-c]pyridin-7-yl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	0.5000	uM
4-amino-N-benzyl-5-cyano-6-ethoxypyridine-2-carboxamide	683374: Inhibition of Mps1-mediated p38 MAPK phosphorylation after 90 mins by DELFIA assay	ic50	0.5290	uM
4-amino-5-cyano-6-ethoxy-N-[(4-methylsulfonylphenyl)methyl]pyridine-2-carboxamide	683374: Inhibition of Mps1-mediated p38 MAPK phosphorylation after 90 mins by DELFIA assay	ic50	0.5680	uM
N-isoquinolin-1-yl-N-[(3R)-piperidin-3-yl]-4-pyrazolo[1,5-a]pyrimidin-3-ylbenzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.6900	uM
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-5-(triazolo[4,5-b]pyridin-3-yl)pyridine-2-carboxamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.7100	uM
N-(3-chloro-2-pyridinyl)-3-[5-(6-methyltriazolo[4,5-b]pyridin-3-yl)-2-pyridinyl]-N-[(3R)-piperidin-3-yl]propanamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.8500	uM
N-(3-chloro-2-pyridinyl)-5-(6-methyltriazolo[4,5-b]pyridin-3-yl)-N-[(3R)-piperidin-3-yl]pyridine-2-carboxamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	0.8500	uM
N-(3-methyl-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-4-pyrazolo[1,5-a]pyrimidin-3-ylpiperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	0.9200	uM
N-isoquinolin-1-yl-4-(1-methylpyrazol-4-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	1.0600	uM
N-isoquinolin-1-yl-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	1.5400	uM
N-(3-methyl-2-pyridinyl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	2.0500	uM
N-(3-chloro-2-pyridinyl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	2.0800	uM
N-(5,8-dihydroisoquinolin-1-yl)-3-(4-methoxyphenyl)-N-[(3R)-piperidin-3-yl]propanamide	1584424: Inhibition of human 80S ribosome-mediated PCSK9 translation expressed in CHO-K1 cells assessed as reduction in PCSK9 secretion	ec50	2.2000	uM
N-isoquinolin-1-yl-3-(4-methoxyphenyl)-N-[(3R)-piperidin-3-yl]propanamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	2.6300	uM
N-(3-chloro-2-pyridinyl)-4-(6-methylpyrazin-2-yl)-N-[(3R)-piperidin-3-yl]benzamide	1497963: Binding affinity to 80S ribosome in human HuH7 cells assessed as inhibition of PCSK9 mRNA translation after overnight incubation by ELISA	ic50	2.8200	uM
N-(3-chloro-2-pyridinyl)-4-[6-(dimethylamino)pyrazin-2-yl]-N-[(3R)-piperidin-3-yl]benzamide	1497963: Binding affinity to 80S ribosome in human HuH7 cells assessed as inhibition of PCSK9 mRNA translation after overnight incubation by ELISA	ic50	2.9700	uM
N-(3-chloro-2-pyridinyl)-4-[6-(methylamino)pyrazin-2-yl]-N-[(3R)-piperidin-3-yl]benzamide	1497963: Binding affinity to 80S ribosome in human HuH7 cells assessed as inhibition of PCSK9 mRNA translation after overnight incubation by ELISA	ic50	3.0800	uM
N-isoquinolin-1-yl-4-(6-methyl-1,2-benzoxazol-3-yl)-N-[(3R)-piperidin-3-yl]piperazine-1-carboxamide	1532845: Binding affinity to 80S ribosome in human HuH7 cells harboring human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	3.5000	uM
N-(3-methylpyrazin-2-yl)-4-phenyl-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	3.6300	uM
4-amino-2-ethoxy-6-(2-phenylethylamino)pyridine-3-carbonitrile	683374: Inhibition of Mps1-mediated p38 MAPK phosphorylation after 90 mins by DELFIA assay	ic50	3.9700	uM
N-(3-chloro-2-pyridinyl)-N-[(3R)-piperidin-3-yl]-6-(triazolo[4,5-b]pyridin-3-yl)pyridine-3-carboxamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	4.1000	uM
N-(4-amino-5-cyano-6-ethoxy-2-pyridinyl)-2-phenylacetamide	683374: Inhibition of Mps1-mediated p38 MAPK phosphorylation after 90 mins by DELFIA assay	ic50	4.2000	uM
N-isoquinolin-1-yl-N-[(3R)-piperidin-3-yl]-4-pyrazin-2-ylbenzamide	1497947: Binding affinity to 80S ribosome in human HuH7 cells harboring pCMV-truncated human PCSK9 (1 to 152 residues)-proLuc assessed as inhibition of PCSK9 mRNA translation after overnight incubation by luciferase reporter gene assay	ic50	4.3000	uM
4-(2-fluorophenyl)-N-(3-methyl-2-pyridinyl)-N-[(3R)-piperidin-3-yl]piperidine-1-carboxamide	1532846: Binding affinity to 80S ribosome in human HeLa cells lysates using human full length PCSK9 encoding mRNAs assessed as inhibition of PCSK9 mRNA translation after 45 mins by Steady-Glo luciferase reporter gene assay	ic50	4.3800	uM
2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide;sulfuric acid	717551: Inhibition of mitochondrial ribosome-mediated protein synthesis in human HeLa cells assessed as {35S]methionine incorporation by autoradiography	ic50	7.4000	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149300: Binding affinity to human RPS27 incubated for 45 mins by Kinobead based pull down assay	kd	7.6532	uM

CTD chemical–gene interactions

64 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects expression, decreases expression, increases expression, affects cotreatment	5
trichostatin A	affects cotreatment, increases expression	3
sodium arsenite	decreases expression, increases expression	3
methylmercuric chloride	increases expression	2
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression, increases expression	2
bisphenol S	decreases expression, increases expression, affects cotreatment	2
Particulate Matter	increases abundance, affects cotreatment, decreases expression	2
FR900359	affects phosphorylation	1
bisphenol F	affects cotreatment, decreases expression	1
triphenyl phosphate	affects expression	1
2-methyl-4-isothiazolin-3-one	increases expression	1
beta-lapachone	increases expression	1
arsenite	affects binding, increases reaction	1
methylparaben	decreases expression	1
coumarin	increases phosphorylation	1
epigallocatechin gallate	increases expression	1
arsenic trichloride	decreases expression, increases abundance, affects cotreatment	1
4-hydroxy-equilenin	increases expression	1
2,3,5-(triglutathion-S-yl)hydroquinone	decreases ADP-ribosylation	1
CD 437	decreases expression	1
chloropicrin	increases expression	1
calfactant	affects cotreatment, increases expression	1
3-(4’-hydroxy-3’-adamantylbiphenyl-4-yl)acrylic acid	decreases expression	1
N-butyrylglucosamine	increases expression	1
dorsomorphin	affects cotreatment, increases expression	1
jinfukang	increases expression	1
LDN 193189	affects cotreatment, decreases expression	1
bisphenol AF	increases expression	1
nabiximols	increases expression	1
Sunitinib	increases expression	1

ChEMBL screening assays

97 unique, capped per target: 97 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL1936525	Binding	Inhibition of MPS1	5-(2-amino-pyrimidin-4-yl)-1H-pyrrole and 2-(2-amino-pyrimidin-4-yl)-1,5,6,7-tetrahydro-pyrrolo[3,2-c]pyridin-4-one derivatives as new classes of selective and orally available Polo-like kinase 1 inhibitors. — Bioorg Med Chem Lett

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.