RPSA2

Summary

RPSA2 (ribosomal protein SA 2, HGNC:36809) is a protein-coding gene on chromosome 19p12, encoding Small ribosomal subunit protein uS2B (A0A8I5KQE6). Required for the assembly and/or stability of the 40S ribosomal subunit.

Predicted to be a structural constituent of ribosome. Predicted to be involved in cytoplasmic translation and ribosomal small subunit assembly. Predicted to be located in several cellular components, including nucleus; plasma membrane; and ribosome. Predicted to be part of cytosolic small ribosomal subunit.

Source: NCBI Gene 388524 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 9 total
• MANE Select transcript: NM_001355283

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:36809
Approved symbol	RPSA2
Name	ribosomal protein SA 2
Location	19p12
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000288920
Ensembl biotype	protein_coding
Entrez	388524

Gene structure

Transcript identifiers

Ensembl transcripts: 23 — 12 protein_coding_CDS_not_defined, 11 protein_coding

ENST00000462186, ENST00000471224, ENST00000475499, ENST00000484897, ENST00000685013, ENST00000685490, ENST00000686829, ENST00000687994, ENST00000688405, ENST00000689525, ENST00000691397, ENST00000692590, ENST00000692739, ENST00000877903, ENST00000877904, ENST00000877905, ENST00000877906, ENST00000917012, ENST00000917013, ENST00000917014, ENST00000949604, ENST00000949605, ENST00000949606

RefSeq mRNA: 4 — MANE Select: NM_001355283 NM_001355283, NM_001355287, NM_001387845, NM_001387846

CCDS: CCDS92575

Canonical transcript exons

ENST00000484897 — 4 exons

Exon	Start	End
ENSE00001619993	23827070	23828868
ENSE00003937975	23763004	23763134
ENSE00004475428	23808719	23808814
ENSE00004475432	23807826	23807939

Expression profiles

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 7.9070 / max 562.8110, expressed in 1038 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
174917	7.7694	1006
174923	0.1375	35

Top tissues by expression

0 total, by Bgee expression score (0-100, higher = more expressed):

Single-cell (SCXA)

Detected in 22 experiment(s), a significant marker in 9.

Experiment	Marker?	Max mean expression
E-CURD-122	yes	2781.61
E-MTAB-11011	yes	2312.70
E-MTAB-9467	yes	1954.88
E-GEOD-139324	yes	1448.74
E-MTAB-7407	yes	844.70
E-MTAB-7051	yes	92.95
E-GEOD-125970	yes	44.22
E-GEOD-135922	yes	27.24
E-HCAD-13	yes	7.35
E-HCAD-23	no	2528.28
E-HCAD-8	no	1514.07
E-GEOD-114530	no	1442.44
E-GEOD-150728	no	983.12
E-CURD-120	no	968.74
E-MTAB-8060	no	830.65

Regulation

Is transcription factor: no

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	rpsa	ENSDARG00000019181
mus_musculus	Rpsa	ENSMUSG00000032518
drosophila_melanogaster	sta	FBGN0003517
caenorhabditis_elegans		WBGENE00004469

Paralogs (1): RPSA (ENSG00000168028)

Protein

Protein identifiers

Small ribosomal subunit protein uS2B — A0A8I5KQE6 (reviewed: A0A8I5KQE6)

Alternative names: 37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 40S ribosomal protein SA, 40S ribosomal protein SA2, 67 kDa laminin receptor, Laminin receptor 1, Laminin-binding protein precursor p40

All UniProt accessions (1): A0A8I5KQE6

UniProt curated annotations — full annotation on UniProt →

Function. Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. Acts as a PPP1R16B-dependent substrate of PPP1CA.

Subunit / interactions. Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK. The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B.

Subcellular location. Cell membrane. Cytoplasm. Nucleus.

Post-translational modifications. Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association. Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.

Miscellaneous. This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage.

Similarity. Belongs to the universal ribosomal protein uS2 family.

RefSeq proteins (4): NP_001342212, NP_001342216, NP_001374774, NP_001374775 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001865	Ribosomal_uS2	Family
IPR005707	Ribosomal_uS2_euk_arc	Family
IPR018130	Ribosomal_uS2_CS	Conserved_site
IPR023591	Ribosomal_uS2_flav_dom_sf	Homologous_superfamily
IPR027498	Ribosomal_uS2_euk	Family
IPR027504	Ribosomal_uS2_vert	Family
IPR032281	Ribosomal_uS2_C	Domain

Pfam: PF00318, PF16122

UniProt features (17 total): region of interest 6, repeat 5, site 2, initiator methionine 1, chain 1, compositionally biased region 1, modified residue 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A0A8I5KQE6-F1	79.22	0.63

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (2): 115–116 (cleavage; by st3; site 1); 133–134 (cleavage; by st3; site 2)

Post-translational modifications (1): 2

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 37 (showing top): GOBP_CYTOPLASMIC_TRANSLATION, GOBP_RIBOSOME_BIOGENESIS, GOBP_RIBOSOME_ASSEMBLY, GOBP_TRANSLATION, GOBP_RIBOSOMAL_SMALL_SUBUNIT_BIOGENESIS, GOMF_STRUCTURAL_CONSTITUENT_OF_RIBOSOME, GOBP_PROTEIN_RNA_COMPLEX_ORGANIZATION, chr19p12, GOBP_ORGANELLE_ASSEMBLY, GOBP_RIBONUCLEOPROTEIN_COMPLEX_BIOGENESIS, GOBP_RIBOSOMAL_SMALL_SUBUNIT_ASSEMBLY, GOCC_RIBOSOME, GOCC_CYTOSOLIC_SMALL_RIBOSOMAL_SUBUNIT, GOCC_SMALL_RIBOSOMAL_SUBUNIT, GOCC_RIBOSOMAL_SUBUNIT

GO Biological Process (4): ribosomal small subunit assembly (GO:0000028), cytoplasmic translation (GO:0002181), translation (GO:0006412), cell adhesion (GO:0007155)

GO Molecular Function (3): structural constituent of ribosome (GO:0003735), laminin receptor activity (GO:0005055), laminin binding (GO:0043236)

GO Cellular Component (8): nucleus (GO:0005634), plasma membrane (GO:0005886), cytosolic small ribosomal subunit (GO:0022627), cytoplasm (GO:0005737), ribosome (GO:0005840), small ribosomal subunit (GO:0015935), membrane (GO:0016020), ribonucleoprotein complex (GO:1990904)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
protein-RNA complex assembly	1
ribosome assembly	1
ribosomal small subunit biogenesis	1
translation	1
peptidyltransferase activity	1
translational initiation	1
translational elongation	1
translational termination	1
macromolecule biosynthetic process	1
protein metabolic process	1
protein biosynthetic process	1
cellular process	1
structural molecule activity	1
ribosome	1
laminin binding	1
cell adhesion mediator activity	1
protein binding	1
extracellular matrix binding	1
intracellular membrane-bounded organelle	1
membrane	1
cell periphery	1
small ribosomal subunit	1
cytosolic ribosome	1
intracellular anatomical structure	1
intracellular membraneless organelle	1
ribosomal subunit	1
protein-containing complex	1

Protein interactions and networks

STRING

0 interactions, top by confidence (×1000):

IntAct

44 interactions, top by confidence:

A	B	Type	Score
METTL1	RPSA2	psi-mi:“MI:0915”(physical association)	0.400
TRAPPC13	RPSA2	psi-mi:“MI:0915”(physical association)	0.400
DND1	RPSA2	psi-mi:“MI:0914”(association)	0.350
P		psi-mi:“MI:0914”(association)	0.350
M		psi-mi:“MI:0914”(association)	0.350
RPL31	RPSA2	psi-mi:“MI:0914”(association)	0.350
SRP14	RPSA2	psi-mi:“MI:0914”(association)	0.350
PARPBP	RPSA2	psi-mi:“MI:0914”(association)	0.350
OR5M11	RPSA2	psi-mi:“MI:0914”(association)	0.350
OR6M1	RPSA2	psi-mi:“MI:0914”(association)	0.350
RNF212	RPSA2	psi-mi:“MI:0914”(association)	0.350
SDR16C5	RPSA2	psi-mi:“MI:0914”(association)	0.350
SGO1	RPSA2	psi-mi:“MI:0914”(association)	0.350
SLA2	RPSA2	psi-mi:“MI:0914”(association)	0.350
SLC25A47	RPSA2	psi-mi:“MI:0914”(association)	0.350
TAS2R50	RPSA2	psi-mi:“MI:0914”(association)	0.350
RPSA2	RSL1D1	psi-mi:“MI:0914”(association)	0.350
TADA1	RPSA2	psi-mi:“MI:0914”(association)	0.350
CNOT3	RPSA2	psi-mi:“MI:0914”(association)	0.350
EPB41L4B	RPSA2	psi-mi:“MI:0914”(association)	0.350
FAM170A	RPSA2	psi-mi:“MI:0914”(association)	0.350
CCSER1	RPSA2	psi-mi:“MI:0914”(association)	0.350
VEGFD	RPSA2	psi-mi:“MI:0914”(association)	0.350
HEY2	RPSA2	psi-mi:“MI:0914”(association)	0.350
MRPS7	RPSA2	psi-mi:“MI:0914”(association)	0.350
HYPK	RPSA2	psi-mi:“MI:0914”(association)	0.350
POLR1D	RPSA2	psi-mi:“MI:0914”(association)	0.350
PPM1B	RPSA2	psi-mi:“MI:0914”(association)	0.350
SPAST	RPSA2	psi-mi:“MI:0914”(association)	0.350
TMEM154	RPSA2	psi-mi:“MI:0914”(association)	0.350

ESM2 similar proteins: A0A8I5KQE6, A1CL63, A1D695, A2I3Z2, A2Q0U8, A3RLT6, A4R188, A6NA00, A6YRY8, A7F2R3, A7RKS5, A8N7C6, B0D174, B0X6V0, B0XWG9, B2B1Z3, B2VY36, B3RPX6, B5DGB6, B5FXT6, B8M6L1, B8N513, B8PAR0, C0NKW7, C0S3U2, C1G1P4, C1GUB6, G1TLT8, P08865, P14206, P26452, P38980, P38981, P38982, P38983, P50890, Q01291, Q0PXX8, Q0UR95, Q2GV75

Diamond homologs: A0A8I5KQE6, A1D695, A2I3Z2, A2Q0U8, A3LRZ0, A3RLT6, A4R188, A4RUK2, A5BUU4, A5DGG9, A6NA00, A6RMY2, A6YRY8, A6ZUM5, A7A0V3, A7RKS5, A7TEU3, A7TIJ7, A8N7C6, A8PXY6, A8Q2H5, A8XSS1, A9UPA2, B0D174, B0X6V0, B0XWG9, B2B1Z3, B2VY36, B3LI22, B3LT19, B3MRX2, B3P9J3, B3RPX6, B4GTK1, B4I9F6, B4JXG8, B4L760, B4MB32, B4NPT0, B4PY37

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

9 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

1928 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000013128 (19:23781122 A>G), RS1000047238 (19:23781822 C>T), RS1000074817 (19:23828525 T>C), RS1000090325 (19:23849796 C>A,T), RS1000092053 (19:23828943 A>G), RS1000191640 (19:23775488 C>T), RS1000198881 (19:23821227 A>C,G), RS1000222593 (19:23781030 A>AGTGGCATGATCT), RS1000222750 (19:23775731 A>G), RS1000227714 (19:23832026 GAA>G,GA), RS1000265333 (19:23865475 G>T), RS1000299297 (19:23805046 A>G), RS1000312765 (19:23815447 G>T), RS1000331581 (19:23758561 G>A), RS1000350912 (19:23836678 A>G)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

20 total (human), top 20 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
aristolochic acid I	increases expression	1
bisphenol A	affects expression	1
sodium arsenate	increases abundance, decreases expression	1
arsenite	affects binding, increases reaction	1
methylparaben	increases expression	1
potassium chromate(VI)	increases expression	1
nickel sulfate	increases expression	1
phenanthrene	decreases expression	1
fipronil	affects cotreatment, increases expression	1
chloropicrin	increases expression	1
jinfukang	increases expression	1
Sunitinib	decreases expression	1
Arsenic	decreases expression, increases abundance	1
Benzo(a)pyrene	decreases methylation	1
Cadmium	increases abundance, increases expression	1
DEET	affects cotreatment, increases expression	1
Tretinoin	decreases expression	1
Vanadates	decreases expression	1
Cadmium Chloride	increases expression, increases abundance	1
Lactic Acid	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.