RRP12

Summary

RRP12 (ribosomal RNA processing 12 homolog, HGNC:29100) is a protein-coding gene on chromosome 10q24.1, encoding RRP12-like protein (Q5JTH9). It is a common-essential gene (DepMap: required in 99.8% of cancer cell lines).

Enables RNA binding activity. Located in cytosol; nucleolus; and plasma membrane.

Source: NCBI Gene 23223 — RefSeq curated summary.

At a glance

• GWAS associations: 8
• Clinical variants (ClinVar): 262 total — 2 pathogenic
• Phenotypes (HPO): 41
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 99.8% of screened cell lines (common-essential)
• MANE Select transcript: NM_015179

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29100
Approved symbol	RRP12
Name	ribosomal RNA processing 12 homolog
Location	10q24.1
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000052749
Ensembl biotype	protein_coding
OMIM	617723
Entrez	23223

Gene structure

Transcript identifiers

Ensembl transcripts: 15 — 8 protein_coding, 4 protein_coding_CDS_not_defined, 3 retained_intron

ENST00000315563, ENST00000370992, ENST00000414986, ENST00000439965, ENST00000465394, ENST00000479317, ENST00000479481, ENST00000487612, ENST00000490815, ENST00000491313, ENST00000536831, ENST00000618765, ENST00000619048, ENST00000621606, ENST00000622320

RefSeq mRNA: 3 — MANE Select: NM_015179 NM_001145114, NM_001284337, NM_015179

CCDS: CCDS44467, CCDS60605, CCDS7457

Canonical transcript exons

ENST00000370992 — 34 exons

Exon	Start	End
ENSE00000502777	97396218	97396301
ENSE00000718386	97379628	97379770
ENSE00000718392	97380799	97380913
ENSE00000718399	97381386	97381483
ENSE00000718413	97385895	97385993
ENSE00000718421	97388252	97388379
ENSE00000718428	97388489	97388624
ENSE00000718462	97400305	97400534
ENSE00000811181	97385166	97385257
ENSE00000811182	97381715	97381826
ENSE00000987268	97372073	97372166
ENSE00001097582	97390423	97390539
ENSE00001097587	97390739	97390844
ENSE00001254203	97370923	97371081
ENSE00003468562	97360546	97360618
ENSE00003479600	97366446	97366621
ENSE00003524850	97366108	97366233
ENSE00003529521	97370455	97370560
ENSE00003538169	97370716	97370796
ENSE00003545233	97373575	97373737
ENSE00003582102	97366742	97366909
ENSE00003587420	97363854	97363903
ENSE00003589133	97369425	97369582
ENSE00003590106	97358943	97359010
ENSE00003591733	97367041	97367132
ENSE00003597661	97372736	97372803
ENSE00003620910	97373046	97373200
ENSE00003622169	97379293	97379414
ENSE00003635083	97358537	97358619
ENSE00003640384	97370167	97370274
ENSE00003668054	97373830	97373894
ENSE00003785810	97393684	97393760
ENSE00003897323	97401093	97401340
ENSE00004282214	97356701	97357196

Expression profiles

Bgee: expression breadth ubiquitous, 227 present calls, max score 92.15.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 23.9470 / max 486.9809, expressed in 1807 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
110933	15.2878	1778
110932	6.4582	1380
110931	1.4497	257
110934	0.5950	351
110927	0.1489	58
110928	0.0074	3

Top tissues by expression

281 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
gastrocnemius	UBERON:0001388	92.15	gold quality
monocyte	CL:0000576	91.63	gold quality
mononuclear cell	CL:0000842	91.53	gold quality
leukocyte	CL:0000738	91.14	gold quality
muscle of leg	UBERON:0001383	91.14	gold quality
bone marrow cell	CL:0002092	90.18	gold quality
hindlimb stylopod muscle	UBERON:0004252	89.60	gold quality
adenohypophysis	UBERON:0002196	89.19	gold quality
granulocyte	CL:0000094	89.18	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	88.86	gold quality
blood	UBERON:0000178	88.64	gold quality
pituitary gland	UBERON:0000007	88.46	gold quality
body of pancreas	UBERON:0001150	88.28	gold quality
muscle organ	UBERON:0001630	88.19	gold quality
apex of heart	UBERON:0002098	87.00	gold quality
left testis	UBERON:0004533	86.71	gold quality
right testis	UBERON:0004534	86.71	gold quality
skin of leg	UBERON:0001511	86.12	gold quality
stromal cell of endometrium	CL:0002255	85.89	gold quality
skin of abdomen	UBERON:0001416	85.79	gold quality
pancreas	UBERON:0001264	85.61	gold quality
testis	UBERON:0000473	85.60	gold quality
heart left ventricle	UBERON:0002084	85.57	gold quality
right atrium auricular region	UBERON:0006631	85.50	gold quality
lower esophagus mucosa	UBERON:0035834	85.34	gold quality
right uterine tube	UBERON:0001302	85.27	gold quality
right adrenal gland cortex	UBERON:0035827	85.18	gold quality
right frontal lobe	UBERON:0002810	85.13	gold quality
left adrenal gland	UBERON:0001234	85.00	gold quality
left adrenal gland cortex	UBERON:0035825	84.94	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.45

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

16 targeting RRP12, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3663-3P	99.84	70.39	798
HSA-MIR-1255A	99.74	68.09	744
HSA-MIR-1255B-5P	99.74	68.16	741
HSA-MIR-593-3P	99.22	67.28	1327
HSA-MIR-6848-5P	98.81	65.49	1126
HSA-MIR-6784-3P	98.39	64.88	662
HSA-MIR-3187-5P	98.36	65.74	1776
HSA-MIR-6862-3P	97.92	64.86	531
HSA-MIR-1285-3P	97.72	67.02	1932
HSA-MIR-5189-5P	97.72	66.96	1814
HSA-MIR-4456	97.50	64.88	1678
HSA-MIR-612	97.26	65.95	1597
HSA-MIR-6860	97.21	66.31	1656
HSA-MIR-4701-5P	96.45	68.41	1121
HSA-MIR-588	96.45	68.36	1127
HSA-MIR-4683	95.29	65.98	631

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 99.8% of screened cell lines, common-essential.

Literature-anchored findings (GeneRIF, showing 1)

• This study demonstrated that RRP12 was crucial for cell survival during cytotoxic stress via the repression of p53 stability. Thus, targeting RRP12 may enhance chemotherapeutic effect in cancers (PMID:26499779)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	rrp12	ENSDARG00000022410
mus_musculus	Rrp12	ENSMUSG00000035049
rattus_norvegicus	Rrp12	ENSRNOG00000048495
drosophila_melanogaster	CG2691	FBGN0030504
caenorhabditis_elegans		WBGENE00021595

Protein

Protein identifiers

RRP12-like protein — Q5JTH9 (reviewed: Q5JTH9)

All UniProt accessions (5): A0A087WYW2, A0A087X0G0, A0A087X1U8, Q5JTH9, H7C1M8

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Nucleus. Nucleolus. Nucleus membrane.

Tissue specificity. Weakly expressed. Expressed at intermediate level in testis and ovary.

Similarity. Belongs to the RRP12 family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q5JTH9-1	1	yes
Q5JTH9-2	2
Q5JTH9-3	3

RefSeq proteins (3): NP_001138586, NP_001271266, NP_055994* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR011989	ARM-like	Homologous_superfamily
IPR012978	HEAT_RRP12	Domain
IPR016024	ARM-type_fold	Homologous_superfamily
IPR052087	RRP12	Family
IPR057860	HEAT_RRP12_N	Domain

Pfam: PF08161, PF25772

UniProt features (122 total): helix 66, strand 14, turn 12, modified residue 9, compositionally biased region 8, region of interest 3, sequence conflict 3, splice variant 2, sequence variant 2, chain 1, transmembrane region 1, cross-link 1

Structure

Experimental structures (PDB)

8 structures.

PDB	Method	Resolution (Å)
7WTU	ELECTRON MICROSCOPY	3
7WTZ	ELECTRON MICROSCOPY	3
7WTT	ELECTRON MICROSCOPY	3.1
7WTX	ELECTRON MICROSCOPY	3.1
7WTS	ELECTRON MICROSCOPY	3.2
7WTW	ELECTRON MICROSCOPY	3.2
7WU0	ELECTRON MICROSCOPY	3.3
7WTV	ELECTRON MICROSCOPY	3.5

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q5JTH9-F1	78.62	0.48

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (10): 49, 66, 72, 77, 88, 97, 1049, 1072, 1080, 71

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 146 (showing top): GRAESSMANN_APOPTOSIS_BY_SERUM_DEPRIVATION_UP, BROWNE_HCMV_INFECTION_16HR_UP, MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP, LIAO_METASTASIS, WANG_RESPONSE_TO_FORSKOLIN_UP, APPIERTO_RESPONSE_TO_FENRETINIDE_UP, ACEVEDO_LIVER_CANCER_UP, WANG_RESPONSE_TO_ANDROGEN_UP, BERENJENO_TRANSFORMED_BY_RHOA_UP, GOCC_NUCLEAR_ENVELOPE, MARSON_BOUND_BY_FOXP3_STIMULATED, GOCC_NUCLEOLUS, GOCC_NUCLEAR_MEMBRANE, ELVIDGE_HIF1A_TARGETS_UP, CROONQUIST_IL6_DEPRIVATION_DN

GO Biological Process (0):

GO Molecular Function (1): RNA binding (GO:0003723)

GO Cellular Component (6): nucleolus (GO:0005730), cytosol (GO:0005829), plasma membrane (GO:0005886), nuclear membrane (GO:0031965), nucleus (GO:0005634), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
nucleic acid binding	1
nuclear lumen	1
intracellular membraneless organelle	1
cytoplasm	1
membrane	1
cell periphery	1
nucleus	1
nuclear envelope	1
organelle membrane	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

2298 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
RRP12	BYSL	Q13895	872
RRP12	RIOK2	Q9BVS4	842
RRP12	PNO1	Q9NRX1	822
RRP12	LTV1	Q96GA3	795
RRP12	UTP20	O75691	781
RRP12	NOP14	P78316	766
RRP12	TSR1	Q2NL82	744
RRP12	NMD3	Q96D46	744
RRP12	PDCD11	Q14690	736
RRP12	RCL1	Q9Y2P8	730
RRP12	NAT10	Q9H0A0	724
RRP12	BMS1	Q14692	722
RRP12	NOB1	Q9ULX3	675
RRP12	GNL2	Q13823	666
RRP12	LSG1	Q9H089	648

IntAct

250 interactions, top by confidence:

A	B	Type	Score
FBL	NOP56	psi-mi:“MI:0914”(association)	0.800
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
H1-1	RRP8	psi-mi:“MI:0914”(association)	0.640
BYSL	PARN	psi-mi:“MI:0914”(association)	0.640
TSR1	RPS3	psi-mi:“MI:0914”(association)	0.640
AURKB	SEC16A	psi-mi:“MI:2364”(proximity)	0.570
CANX	PGRMC1	psi-mi:“MI:0914”(association)	0.570
RPS6	IPO7	psi-mi:“MI:0914”(association)	0.530
FGF3	GTPBP10	psi-mi:“MI:0914”(association)	0.530
ZNF324B	ZNF316	psi-mi:“MI:0914”(association)	0.530
H1-6	ZNF724	psi-mi:“MI:0914”(association)	0.530
RRP8	NVL	psi-mi:“MI:0914”(association)	0.530
H1-4	IGF2BP3	psi-mi:“MI:0914”(association)	0.530
RPS2	MPHOSPH10	psi-mi:“MI:0914”(association)	0.530
PRR11	NVL	psi-mi:“MI:0914”(association)	0.530
SCN3B	ABCC5	psi-mi:“MI:0914”(association)	0.530
RPL7	ZBTB24	psi-mi:“MI:0914”(association)	0.530
PDGFB	DKC1	psi-mi:“MI:0914”(association)	0.530
TSR1	PARN	psi-mi:“MI:0914”(association)	0.530
WDR5B	TCP1	psi-mi:“MI:0914”(association)	0.530
RPS3	ZNF316	psi-mi:“MI:0914”(association)	0.530
PBXIP1	GOLIM4	psi-mi:“MI:0914”(association)	0.530
H2BC26	PPM1G	psi-mi:“MI:0914”(association)	0.530

BioGRID (402): RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), RRP12 (Affinity Capture-MS), BRIX1 (Co-fractionation), DDX24 (Co-fractionation)

ESM2 similar proteins: A0JMW2, A2VE70, A5WW24, A7E2Y6, B9EJR8, E0CZ22, E1BP36, E7FBU4, O35638, O43156, O70576, O75155, Q08AM6, Q0P5A6, Q0V9L1, Q16401, Q5IFJ8, Q5JTH9, Q5R6L5, Q5ZIW5, Q5ZKD5, Q66L58, Q68F38, Q6DCF2, Q6P5B0, Q6ZQ73, Q7TMY7, Q80W92, Q80WQ2, Q84ZC0, Q86Y56, Q8C0Y0, Q8K2V6, Q8NDA8, Q8WVM7, Q91V83, Q96T76, Q99M76, Q9BPX3, Q9D071

Diamond homologs: Q5JTH9, Q5ZKD5, Q6P5B0

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 217 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Eukaryotic Translation Initiation	12	25.5×	6e-13
Cap-dependent Translation Initiation	12	25.5×	6e-13
SARS-CoV-1 modulates host translation machinery	12	25.5×	6e-13
Eukaryotic Translation Elongation	12	23.1×	2e-12
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S	12	22.5×	3e-12
Influenza Viral RNA Transcription and Replication	13	19.3×	2e-12
Nonsense-Mediated Decay (NMD)	12	19.3×	2e-11
SARS-CoV-2 modulates host translation machinery	12	18.5×	3e-11

GO biological processes:

GO term	Partners	Fold	FDR
negative regulation of DNA recombination	6	35.7×	2e-06
chromosome condensation	7	31.2×	3e-07
cytoplasmic translation	22	21.6×	2e-20
maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	6	21.4×	4e-05
ribosomal small subunit biogenesis	15	18.1×	1e-12
translation	22	12.0×	6e-15
ribosomal large subunit biogenesis	5	11.7×	7e-03
rRNA processing	15	11.2×	1e-09

Disease & clinical

Clinical variants and AI predictions

ClinVar

262 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	2
Likely pathogenic	0
Uncertain significance	213
Likely benign	14
Benign	2

Top pathogenic / likely-pathogenic (2)

Variant ID	HGVS	Classification
4538524	NM_015179.4(RRP12):c.1429G>A (p.Glu477Lys)	Pathogenic
4538525	NM_015179.4(RRP12):c.2634T>G (p.Phe878Leu)	Pathogenic

SpliceAI

5183 predictions. Top by Δscore:

Variant	Effect	Δscore
10:97357194:TTCC:T	acceptor_loss	1.0000
10:97358535:ACCT:A	donor_loss	1.0000
10:97358536:CCTG:C	donor_gain	1.0000
10:97358616:CTTT:C	acceptor_gain	1.0000
10:97358617:TTT:T	acceptor_gain	1.0000
10:97358618:TT:T	acceptor_gain	1.0000
10:97358618:TTCTG:T	acceptor_loss	1.0000
10:97358619:TCT:T	acceptor_loss	1.0000
10:97358620:C:CC	acceptor_gain	1.0000
10:97358620:C:CG	acceptor_loss	1.0000
10:97358621:T:A	acceptor_loss	1.0000
10:97358624:T:C	acceptor_gain	1.0000
10:97358624:T:TC	acceptor_gain	1.0000
10:97358629:C:CT	acceptor_gain	1.0000
10:97358629:C:T	acceptor_gain	1.0000
10:97358630:A:T	acceptor_gain	1.0000
10:97358938:CTTA:C	donor_loss	1.0000
10:97358940:TA:T	donor_loss	1.0000
10:97358941:A:AC	donor_gain	1.0000
10:97358941:AC:A	donor_gain	1.0000
10:97358942:C:CC	donor_gain	1.0000
10:97358942:C:CG	donor_loss	1.0000
10:97358942:CC:C	donor_gain	1.0000
10:97359006:TCCAG:T	acceptor_gain	1.0000
10:97359007:CCAG:C	acceptor_gain	1.0000
10:97359007:CCAGC:C	acceptor_gain	1.0000
10:97359008:CAG:C	acceptor_gain	1.0000
10:97359008:CAGC:C	acceptor_gain	1.0000
10:97359009:AG:A	acceptor_gain	1.0000
10:97359009:AGCTA:A	acceptor_loss	1.0000

AlphaMissense

8441 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
10:97396283:C:G	A130P	0.999
10:97396288:A:G	L128P	0.999
10:97371047:C:G	R793P	0.998
10:97388611:G:T	A256D	0.998
10:97388620:C:G	R253P	0.998
10:97390491:A:G	W229R	0.998
10:97390491:A:T	W229R	0.998
10:97393705:A:G	L170P	0.998
10:97396219:A:G	L151P	0.998
10:97396232:A:G	Y147H	0.998
10:97396282:G:T	A130D	0.998
10:97396291:A:T	V127D	0.998
10:97366478:A:G	F1120S	0.997
10:97379389:A:G	W568R	0.997
10:97379389:A:T	W568R	0.997
10:97385206:A:G	W390R	0.997
10:97385206:A:T	W390R	0.997
10:97388612:C:G	A256P	0.997
10:97390842:A:T	V178D	0.997
10:97393702:A:G	L171P	0.997
10:97393717:G:T	A166D	0.997
10:97396232:A:C	Y147D	0.997
10:97396279:A:T	V131D	0.997
10:97396286:C:G	A129P	0.997
10:97396294:G:T	A126D	0.997
10:97396295:C:G	A126P	0.997
10:97400354:A:G	F107S	0.997
10:97370784:A:G	C839R	0.996
10:97385253:A:G	L374P	0.996
10:97390530:A:G	C216R	0.996

dbSNP variants (sampled 300 via entrez): RS1000066791 (10:97399081 C>T), RS1000107939 (10:97381032 C>A,T), RS1000115977 (10:97397883 C>G,T), RS1000269205 (10:97375029 T>G), RS1000323402 (10:97362707 G>A,C), RS1000327604 (10:97369260 G>A), RS1000339375 (10:97356508 C>T), RS1000348273 (10:97386959 C>T), RS1000413060 (10:97395460 G>A), RS1000669212 (10:97397205 G>C), RS1000705919 (10:97368181 C>A,T), RS1000723968 (10:97375359 C>A,T), RS1000747209 (10:97358486 C>T), RS1000790761 (10:97395205 T>C), RS1000815618 (10:97390906 C>A,T)

Disease associations

OMIM: gene MIM:617723 | disease phenotypes: MIM:621452

GenCC curated gene-disease

Mondo (1): basal ganglia calcification, idiopathic, 11, autosomal recessive (MONDO:0980939)

Orphanet (0):

HPO phenotypes

41 total (30 of 41 shown, HPO-id order):

HPO	Term
HP:0000007	Autosomal recessive inheritance
HP:0000252	Microcephaly
HP:0000360	Tinnitus
HP:0000518	Cataract
HP:0000712	Emotional lability
HP:0000739	Anxiety
HP:0001251	Ataxia
HP:0001257	Spasticity
HP:0001260	Dysarthria
HP:0001268	Mental deterioration
HP:0001300	Parkinsonism
HP:0001332	Dystonia
HP:0001873	Thrombocytopenia
HP:0002063	Rigidity
HP:0002067	Bradykinesia
HP:0002072	Chorea
HP:0002135	Basal ganglia calcification
HP:0002197	Generalized-onset seizure
HP:0002273	Tetraparesis
HP:0002283	Global brain atrophy
HP:0002311	Incoordination
HP:0002315	Headache
HP:0002321	Vertigo
HP:0002352	Leukoencephalopathy
HP:0002354	Memory impairment
HP:0002425	Anarthria
HP:0002505	Loss of ambulation
HP:0002514	Cerebral calcification
HP:0003584	Late onset
HP:0003596	Middle age onset

GWAS associations

8 associations (top):

Study	Trait	p-value
GCST004608_163	Granulocyte percentage of myeloid white cells	3.000000e-10
GCST004609_17	Monocyte percentage of white cells	4.000000e-16
GCST004625_182	Monocyte count	1.000000e-27
GCST004904_162	Body mass index	7.000000e-09
GCST90002393_338	Monocyte count	3.000000e-50
GCST90002394_453	Monocyte percentage of white cells	6.000000e-33
GCST90002398_183	Neutrophil count	5.000000e-12
GCST90002407_311	White blood cell count	3.000000e-14

EFO canonical traits (5, from GWAS)

EFO ID	Trait name
EFO:0007997	granulocyte percentage of myeloid white cells
EFO:0007989	monocyte percentage of leukocytes
EFO:0005091	monocyte count
EFO:0004340	body mass index
EFO:0004833	neutrophil count

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067385 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
9.59	Kd	0.256	nM	CHEMBL5653589
9.59	ED50	0.256	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149322: Binding affinity to human RRP12 incubated for 45 mins by Kinobead based pull down assay	kd	0.0003	uM

CTD chemical–gene interactions

69 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cisplatin	affects expression, decreases expression, increases expression	3
Cyclosporine	increases expression	3
Aflatoxin B1	affects cotreatment, decreases expression, decreases methylation, increases expression	3
bisphenol A	decreases expression, affects expression, affects cotreatment	2
Acetaminophen	decreases expression, increases expression	2
Estradiol	increases expression	2
aristolochic acid I	increases expression	1
FR900359	affects phosphorylation	1
bisphenol F	affects cotreatment, decreases expression	1
4-hydroxyphenyl 4-isopropoxyphenylsulfone	increases expression	1
dicrotophos	increases expression	1
alpha phellandrene	decreases expression	1
triphenyl phosphate	affects expression	1
deoxynivalenol	increases expression	1
2-methyl-4-isothiazolin-3-one	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
2-amino-9H-pyrido(2,3-b)indole	increases expression	1
sodium arsenite	increases abundance, increases expression	1
perfluorooctanoic acid	increases expression	1
potassium chromate(VI)	increases expression	1
hydroquinone	decreases expression	1
exemestane	increases expression	1
di-n-butylphosphoric acid	affects expression	1
azoxystrobin	decreases expression	1
perfluoro-n-nonanoic acid	increases expression	1
2-palmitoylglycerol	increases expression	1
K 7174	increases expression	1
ICG 001	decreases expression	1
abrine	increases expression	1
bisphenol S	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652364	Binding	Binding affinity to human RRP12 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.