RTRAF

Summary

RTRAF (RNA transcription, translation and transport factor, HGNC:23169) is a protein-coding gene on chromosome 14q22.1, encoding tRNA-splicing ligase complex subunit RTRAF (Q9Y224). Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3’,5’-phosphodiester. It is a selective cancer dependency (DepMap: 39.7% of cell lines).

Enables RNA binding activity; RNA polymerase II complex binding activity; and identical protein binding activity. Involved in negative regulation of protein kinase activity; positive regulation of transcription by RNA polymerase II; and tRNA splicing, via endonucleolytic cleavage and ligation. Located in microtubule cytoskeleton; nucleoplasm; and perinuclear region of cytoplasm. Part of tRNA-splicing ligase complex.

Source: NCBI Gene 51637 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 16 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 39.7% of screened cell lines
• MANE Select transcript: NM_016039

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:23169
Approved symbol	RTRAF
Name	RNA transcription, translation and transport factor
Location	14q22.1
Locus type	gene with protein product
Status	Approved
Aliases	CGI-99, RLLM1, CLE, CLE7, LCRP369, hCLE1
Ensembl gene	ENSG00000087302
Ensembl biotype	protein_coding
OMIM	610858
Entrez	51637

Gene structure

Transcript identifiers

Ensembl transcripts: 16 — 12 protein_coding, 3 retained_intron, 1 nonsense_mediated_decay

ENST00000261700, ENST00000553362, ENST00000553479, ENST00000553707, ENST00000555319, ENST00000556760, ENST00000557553, ENST00000902518, ENST00000902519, ENST00000902520, ENST00000920645, ENST00000920646, ENST00000920647, ENST00000920648, ENST00000920649, ENST00000942315

RefSeq mRNA: 1 — MANE Select: NM_016039 NM_016039

CCDS: CCDS9705

Canonical transcript exons

ENST00000261700 — 8 exons

Exon	Start	End
ENSE00000657239	51991317	51991441
ENSE00000657245	52004194	52004242
ENSE00000854696	51989546	51989700
ENSE00001227381	51993723	51993822
ENSE00001365973	52004362	52010694
ENSE00003629113	51999708	51999796
ENSE00003661809	52001798	52001866
ENSE00003684928	51998494	51998580

Expression profiles

Bgee: expression breadth ubiquitous, 288 present calls, max score 99.82.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 247.9264 / max 1457.4527, expressed in 1827 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
139598	243.2837	1827
139599	4.1383	1504
139601	0.4930	228
139600	0.0113	7

Top tissues by expression

288 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	99.82	gold quality
oocyte	CL:0000023	99.65	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	99.48	gold quality
right testis	UBERON:0004534	99.46	gold quality
left testis	UBERON:0004533	99.44	gold quality
cartilage tissue	UBERON:0002418	99.03	gold quality
ganglionic eminence	UBERON:0004023	99.02	gold quality
calcaneal tendon	UBERON:0003701	98.99	gold quality
embryo	UBERON:0000922	98.98	gold quality
palpebral conjunctiva	UBERON:0001812	98.96	gold quality
adult organism	UBERON:0007023	98.93	gold quality
heart right ventricle	UBERON:0002080	98.89	gold quality
body of pancreas	UBERON:0001150	98.88	gold quality
tibia	UBERON:0000979	98.84	gold quality
heart left ventricle	UBERON:0002084	98.83	gold quality
cortical plate	UBERON:0005343	98.83	gold quality
cardiac ventricle	UBERON:0002082	98.82	gold quality
lower esophagus muscularis layer	UBERON:0035833	98.82	gold quality
testis	UBERON:0000473	98.81	gold quality
lower esophagus	UBERON:0013473	98.81	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	98.74	gold quality
male germ cell	CL:0000015	98.73	gold quality
esophagus squamous epithelium	UBERON:0006920	98.71	gold quality
heart	UBERON:0000948	98.70	gold quality
body of stomach	UBERON:0001161	98.69	gold quality
right atrium auricular region	UBERON:0006631	98.69	gold quality
muscle layer of sigmoid colon	UBERON:0035805	98.67	gold quality
sperm	CL:0000019	98.66	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	98.66	gold quality
right adrenal gland cortex	UBERON:0035827	98.65	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-MTAB-10042	yes	4.47
E-MTAB-7606	no	511.52
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

14 targeting RTRAF, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-548E-5P	99.89	72.73	4486
HSA-MIR-3133	99.81	70.92	3506
HSA-MIR-542-3P	99.34	67.58	1270
HSA-MIR-4504	99.10	69.14	1328
HSA-MIR-6770-5P	98.97	66.76	1853
HSA-MIR-3124-3P	98.87	68.95	2123
HSA-MIR-4477A	98.83	69.75	2952
HSA-MIR-3908	98.75	67.31	1160
HSA-MIR-4501	98.72	67.19	921
HSA-MIR-4703-5P	98.53	70.13	1645
HSA-MIR-3942-5P	98.52	69.51	1517
HSA-MIR-216B-5P	97.16	66.76	1126
HSA-MIR-517-5P	97.13	68.43	781

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 39.7% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 13)

• A novel protein CGI-99 is isolated, which may be involved in the functional regulation of human ninein in the centrosome structure and may also be important in brain development and tumorigenesis. (PMID:15147888)
• c14orf166 was identified asa novel metastasis-associated protein, and the roles of radixin, moesin and c14orf166 in pancreatic cancer metastasis deserve further investigations. (PMID:19152423)
• spatial interactions of hnRNPH1, NF45, and C14orf166 with HCVc174 likely modulate HCV or cellular functions during acute and chronic HCV infection (PMID:21823664)
• Silencing of CLE causes reduction of influenza A virus polymerase activity, viral RNA transcription and replication, virus titer, and viral particle production. (PMID:21900157)
• hCLE/C14orf166 associates with DDX1, HSPC117, and FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. (PMID:24608264)
• Overexpression of chromosome 14 open reading frame 166 correlates with disease progression in nasopharyngeal carcinoma. (PMID:25964093)
• C14ORF166 may represent a biomarker of pelvic lymph node metastasis in uterine cervical cancer (PMID:26219895)
• C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles. (PMID:26864902)
• C14orf166 could be a novel prognostic biomarker of breast cancer, it also contributes to cell proliferation by regulating G1/S transition. (PMID:26883017)
• C14orf166 promotes bladder cancer cell proliferation and can be a novel prognostic biomarker for patients with bladder cancer. (PMID:26905879)
• the two structural homologs FAM98A and FAM98B included in a novel complex with DDX1 and C14orf166 are required for PRMT1 expression in colorectal cancer cell lines (PMID:28040436)
• Molecular architecture of the human tRNA ligase complex. (PMID:34854379)
• An in silico prediction of interaction models of influenza A virus PA and human C14orf166 protein from yeast-two-hybrid screening data. (PMID:37265372)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	rtraf	ENSDARG00000009253
mus_musculus	Rtraf	ENSMUSG00000021807
rattus_norvegicus	Rtraf	ENSRNOG00000000340
drosophila_melanogaster	CG31249	FBGN0051249
caenorhabditis_elegans		WBGENE00008457

Protein

Protein identifiers

tRNA-splicing ligase complex subunit RTRAF — Q9Y224 (reviewed: Q9Y224)

Alternative names: CLE7 homolog, RNA transcription, translation and transport factor protein

All UniProt accessions (5): Q9Y224, G3V4C6, G3V4E7, H0YJB9, Q549M8

UniProt curated annotations — full annotation on UniProt →

Function. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3’,5’-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport. (Microbial infection) In case of infection by influenza virus A (IVA), is involved in viral replication.

Subunit / interactions. Homodimer. Component of the tRNA-splicing ligase core complex composed of the catalytic subunit RTCB and the accessory proteins DDX1, C2orf49/Ashwin/ASW, FAM98B and RTRAF/CGI-99. Interacts with FAM98A (via N- and C-terminus). Interacts with NIN; which may prevent phosphorylation of NIN. Interacts with POLR2A. (Microbial infection) Interacts with influenza A virus (IAV) RNA polymerase subunits PA, PB1 and PB2, and nucleocapsid NP. Associates with IAV polymerase complexes both in the nucleus and cytosol. Associates with IAV ribonucleoproteins (vRNP) packaged in virions. Interacts with hepatitis C virus core protein p19.

Subcellular location. Nucleus. Cytoplasm. Cytosol. Perinuclear region. Cytoskeleton. Microtubule organizing center. Centrosome Nucleus.

Tissue specificity. Widely expressed. Expressed at high level in heart and skeletal muscle. Expressed at intermediate level in liver, pancreas, fetal brain and fetal lung. Weakly expressed in adult brain, adult lung, placenta, fetal liver and fetal kidney. Overexpressed in many brain tumors.

Induction. (Microbial infection) Up-regulated specifically following influenza A virus (IAV) infection in a viral replication-dependent manner (at protein level).

Similarity. Belongs to the RTRAF family.

RefSeq proteins (1): NP_057123* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019265	RTRAF	Family

Pfam: PF10036

UniProt features (11 total): helix 7, modified residue 3, chain 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
7P3A	X-RAY DIFFRACTION	2

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9Y224-F1	72.09	0.00

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 20, 62, 98

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-6784531	tRNA processing in the nucleus
R-HSA-72306	tRNA processing
R-HSA-8953854	Metabolism of RNA

MSigDB gene sets: 159 (showing top): YAATNRNNNYNATT_UNKNOWN, GOBP_REGULATION_OF_PHOSPHORYLATION, GOBP_NEGATIVE_REGULATION_OF_KINASE_ACTIVITY, MODULE_151, STARK_PREFRONTAL_CORTEX_22Q11_DELETION_DN, GOBP_TRNA_METABOLIC_PROCESS, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, DARWICHE_SKIN_TUMOR_PROMOTER_DN, GOBP_REGULATION_OF_TRANSFERASE_ACTIVITY, DARWICHE_PAPILLOMA_RISK_LOW_DN, DARWICHE_PAPILLOMA_RISK_HIGH_DN, DARWICHE_SQUAMOUS_CELL_CARCINOMA_DN, CAGCTG_AP4_Q5, GOCC_MICROTUBULE_ORGANIZING_CENTER, MARTINEZ_RB1_TARGETS_DN

GO Biological Process (4): tRNA splicing, via endonucleolytic cleavage and ligation (GO:0006388), negative regulation of protein kinase activity (GO:0006469), positive regulation of transcription by RNA polymerase II (GO:0045944), RNA transport (GO:0050658)

GO Molecular Function (4): RNA polymerase II complex binding (GO:0000993), RNA binding (GO:0003723), identical protein binding (GO:0042802), protein binding (GO:0005515)

GO Cellular Component (9): nucleus (GO:0005634), nucleoplasm (GO:0005654), cytoplasm (GO:0005737), centrosome (GO:0005813), cytosol (GO:0005829), perinuclear region of cytoplasm (GO:0048471), tRNA-splicing ligase complex (GO:0072669), mitotic spindle (GO:0072686), cytoskeleton (GO:0005856)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
tRNA processing	1
Metabolism of RNA	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	4
cytoplasm	2
RNA splicing, via endonucleolytic cleavage and ligation	1
tRNA processing	1
negative regulation of protein phosphorylation	1
protein kinase activity	1
negative regulation of kinase activity	1
regulation of protein kinase activity	1
regulation of transcription by RNA polymerase II	1
transcription by RNA polymerase II	1
positive regulation of DNA-templated transcription	1
nucleic acid transport	1
establishment of RNA localization	1
RNA polymerase core enzyme binding	1
nucleic acid binding	1
protein binding	1
binding	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
intracellular anatomical structure	1
centriole	1
microtubule organizing center	1
intracellular protein-containing complex	1
catalytic complex	1
spindle	1
intracellular membraneless organelle	1

Protein interactions and networks

STRING

1680 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
RTRAF	FAM98B	Q52LJ0	996
RTRAF	DDX1	Q92499	992
RTRAF	RTCB	Q9Y3I0	943
RTRAF	NIN	Q8N4C6	907
RTRAF	C2orf49	Q9BVC5	896
RTRAF	RMDN3	Q96TC7	730
RTRAF	FAM98A	Q8NCA5	727
RTRAF	POLR2A	P24928	620
RTRAF	ZBTB8OS	Q8IWT0	580
RTRAF	TAF15	Q92804	495
RTRAF	GSK3B	P49841	481
RTRAF	SMU1	Q2TAY7	476
RTRAF	TXNDC16	Q9P2K2	455
RTRAF	ILF2	Q12905	449
RTRAF	LSM12	Q3MHD2	444

IntAct

200 interactions, top by confidence:

A	B	Type	Score
FAM98A	RTRAF	psi-mi:“MI:0915”(physical association)	0.810
RTRAF	FAM98A	psi-mi:“MI:0915”(physical association)	0.810
RTRAF	RTCB	psi-mi:“MI:0915”(physical association)	0.750
RTCB	RTRAF	psi-mi:“MI:0915”(physical association)	0.750
RTRAF	RTRAF	psi-mi:“MI:0915”(physical association)	0.730
RTRAF	RTRAF	psi-mi:“MI:0407”(direct interaction)	0.730
RTRAF	DDX1	psi-mi:“MI:0915”(physical association)	0.720
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
DDX1	FAM98B	psi-mi:“MI:0915”(physical association)	0.670
CFTR	HAX1	psi-mi:“MI:0914”(association)	0.610
RTRAF	PA	psi-mi:“MI:0915”(physical association)	0.580
PA	RTRAF	psi-mi:“MI:0915”(physical association)	0.580
RTRAF	PA	psi-mi:“MI:0915”(physical association)	0.570
PA	RTRAF	psi-mi:“MI:0915”(physical association)	0.570
NIN	RTRAF	psi-mi:“MI:0915”(physical association)	0.560
RTRAF	NIN	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (306): C14orf166 (Affinity Capture-MS), C14orf166 (Affinity Capture-MS), C14orf166 (Two-hybrid), C14orf166 (Affinity Capture-MS), C14orf166 (Affinity Capture-MS), C14orf166 (Affinity Capture-MS), C14orf166 (Affinity Capture-MS), C14orf166 (Affinity Capture-MS), ACTR3B (Co-fractionation), AHNAK (Co-fractionation), C14orf166 (Co-fractionation), C14orf166 (Co-fractionation), C14orf166 (Co-fractionation), C14orf166 (Co-fractionation), C14orf166 (Co-fractionation)

ESM2 similar proteins: A6H7C9, A7S7F2, A8WVM4, A9UL78, A9UNU6, G5EDM7, G5EG51, H2QII6, O14715, O44740, O54825, O60071, P30641, P31376, P38333, P49792, P51406, Q03280, Q0IHJ3, Q10573, Q13895, Q18508, Q20932, Q21341, Q291E4, Q54IS0, Q5E9N0, Q5R8I2, Q63ZS0, Q6BER5, Q6DFE2, Q6FRS1, Q6FS52, Q7ZUH1, Q80WL2, Q8MYL1, Q8RWS4, Q95Y84, Q96LK0, Q99666

Diamond homologs: Q5R8I2, Q63ZS0, Q7ZUH1, Q9CQE8, Q9Y224

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 174 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

GO biological processes:

GO term	Partners	Fold	FDR
stress granule assembly	5	21.8×	6e-04
intrinsic apoptotic signaling pathway	6	15.6×	6e-04
mRNA stabilization	5	13.3×	5e-03
G1/S transition of mitotic cell cycle	8	11.6×	1e-04
positive regulation of fibroblast proliferation	5	10.7×	9e-03
positive regulation of miRNA transcription	5	10.5×	9e-03
negative regulation of translation	7	9.9×	1e-03
cytoplasmic translation	6	8.1×	9e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

16 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1381 predictions. Top by Δscore:

Variant	Effect	Δscore
14:51989697:AAAGG:A	donor_loss	1.0000
14:51989698:AAGG:A	donor_loss	1.0000
14:51989700:GGT:G	donor_loss	1.0000
14:51989701:GTGAG:G	donor_loss	1.0000
14:51991310:A:AG	acceptor_gain	1.0000
14:51991315:A:AG	acceptor_gain	1.0000
14:51991315:AGAT:A	acceptor_gain	1.0000
14:51991316:G:GC	acceptor_gain	1.0000
14:51991316:GA:G	acceptor_gain	1.0000
14:51991316:GAT:G	acceptor_gain	1.0000
14:51991316:GATG:G	acceptor_gain	1.0000
14:51991316:GATGA:G	acceptor_gain	1.0000
14:51991442:G:GG	donor_gain	1.0000
14:51991442:GTAAT:G	donor_loss	1.0000
14:51991443:T:G	donor_loss	1.0000
14:51991454:G:GT	donor_gain	1.0000
14:51993720:C:G	acceptor_gain	1.0000
14:51993721:A:AG	acceptor_gain	1.0000
14:51993721:A:G	acceptor_loss	1.0000
14:51993722:G:GA	acceptor_gain	1.0000
14:51993722:GT:G	acceptor_gain	1.0000
14:51993722:GTAT:G	acceptor_gain	1.0000
14:51993722:GTATC:G	acceptor_gain	1.0000
14:51993819:AATGG:A	donor_loss	1.0000
14:51993820:ATGG:A	donor_loss	1.0000
14:51993821:TGGTA:T	donor_loss	1.0000
14:51993823:G:GA	donor_loss	1.0000
14:51993824:TA:T	donor_loss	1.0000
14:51998488:TTATA:T	acceptor_loss	1.0000
14:51998490:ATAGC:A	acceptor_loss	1.0000

AlphaMissense

1622 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
14:51991346:T:A	W31R	1.000
14:51991346:T:C	W31R	1.000
14:51991350:T:C	L32P	1.000
14:51991355:G:C	D34H	1.000
14:51991356:A:T	D34V	1.000
14:51991359:A:C	Q35P	1.000
14:51991368:G:C	R38T	1.000
14:51991368:G:T	R38M	1.000
14:51991369:G:C	R38S	1.000
14:51991369:G:T	R38S	1.000
14:51991389:G:C	R45T	1.000
14:51991389:G:T	R45I	1.000
14:51991390:A:C	R45S	1.000
14:51991390:A:T	R45S	1.000
14:51991401:G:C	R49T	1.000
14:51991402:A:C	R49S	1.000
14:51991402:A:T	R49S	1.000
14:51991421:T:A	W56R	1.000
14:51991421:T:C	W56R	1.000
14:51993799:T:A	V88D	1.000
14:51999746:G:C	A138P	1.000
14:52004383:C:A	A201D	1.000
14:52004392:T:C	L204P	1.000
14:52004416:T:C	L212P	1.000
14:52004419:G:C	R213T	1.000
14:52004419:G:T	R213I	1.000
14:52004420:A:C	R213S	1.000
14:52004420:A:T	R213S	1.000
14:52004425:T:C	L215P	1.000
14:52004429:G:C	Q216H	1.000

dbSNP variants (sampled 300 via entrez): RS1000041219 (14:51990811 CTTA>C), RS1000056572 (14:51992827 T>C), RS1000173336 (14:52005175 T>G), RS1000213083 (14:52004845 T>C), RS1000237193 (14:51996727 C>A), RS1000329013 (14:52004587 A>G), RS1000550596 (14:52008134 A>G), RS1000581797 (14:52007941 C>G,T), RS1000639427 (14:51998860 G>A), RS1000824771 (14:52003012 A>C), RS1000886833 (14:52010492 A>C), RS1000974984 (14:51987800 C>G), RS1001020704 (14:51992568 A>G), RS1001071194 (14:51994249 A>G), RS1001131920 (14:51994029 C>G)

Disease associations

OMIM: gene MIM:610858 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST004412_11	Craniofacial microsomia	1.000000e-07

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067445 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

28 total (human), top 28 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects cotreatment, decreases methylation, decreases expression	3
Cyclosporine	increases expression	2
2,4,6-tribromophenol	decreases expression	1
arsenite	affects binding, increases reaction	1
tetrabromobisphenol A	decreases expression	1
chloropicrin	increases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
Grape Seed Proanthocyanidins	affects cotreatment, decreases expression	1
Resveratrol	increases expression, affects cotreatment	1
Fulvestrant	affects cotreatment, decreases methylation	1
Air Pollutants	increases abundance, decreases expression	1
Aminoglutethimide	decreases expression	1
Arsenic	decreases expression	1
Benzo(a)pyrene	affects methylation	1
Catechin	affects cotreatment, decreases expression	1
Cisplatin	increases expression	1
Doxorubicin	decreases expression	1
Enzyme Inhibitors	decreases activity, increases O-linked glycosylation	1
Ivermectin	decreases expression	1
Lead	affects expression	1
Plant Extracts	increases expression, affects cotreatment	1
Tretinoin	decreases expression	1
Josamycin	affects response to substance	1
Aflatoxin B1	decreases methylation	1
Sodium Selenite	increases expression	1
Antirheumatic Agents	increases expression	1
Copper Sulfate	decreases expression	1
Particulate Matter	decreases expression, increases abundance	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652876	Binding	Binding affinity to human C14orf166 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.