SCAMP3

Summary

SCAMP3 (secretory carrier membrane protein 3, HGNC:10565) is a protein-coding gene on chromosome 1q22, encoding Secretory carrier-associated membrane protein 3 (O14828). Functions in post-Golgi recycling pathways.

This gene encodes an integral membrane protein that belongs to the secretory carrier membrane protein family. The encoded protein functions as a carrier to the cell surface in post-golgi recycling pathways. This protein is also involved in protein trafficking in endosomal pathways. Two transcript variants encoding different isoforms have been found for this gene.

Source: NCBI Gene 10067 — RefSeq curated summary.

At a glance

• GWAS associations: 18
• Clinical variants (ClinVar): 71 total
• Druggable target: yes
• MANE Select transcript: NM_005698

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:10565
Approved symbol	SCAMP3
Name	secretory carrier membrane protein 3
Location	1q22
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000116521
Ensembl biotype	protein_coding
OMIM	606913
Entrez	10067

Gene structure

Transcript identifiers

Ensembl transcripts: 36 — 26 protein_coding, 7 retained_intron, 3 nonsense_mediated_decay

ENST00000302631, ENST00000355379, ENST00000462151, ENST00000465312, ENST00000472397, ENST00000478737, ENST00000480219, ENST00000490999, ENST00000497470, ENST00000714213, ENST00000714214, ENST00000714215, ENST00000714216, ENST00000714217, ENST00000714218, ENST00000714219, ENST00000714220, ENST00000714221, ENST00000714222, ENST00000714223, ENST00000714224, ENST00000880564, ENST00000880565, ENST00000880566, ENST00000880567, ENST00000880568, ENST00000880569, ENST00000880570, ENST00000880571, ENST00000880572, ENST00000929050, ENST00000929051, ENST00000929052, ENST00000929053, ENST00000945653, ENST00000945654

RefSeq mRNA: 2 — MANE Select: NM_005698 NM_005698, NM_052837

CCDS: CCDS1105, CCDS1106

Canonical transcript exons

ENST00000302631 — 9 exons

Exon	Start	End
ENSE00001881861	155258826	155258954
ENSE00003508700	155257285	155257386
ENSE00003592324	155257498	155257657
ENSE00004023141	155260330	155260450
ENSE00004023143	155260537	155260659
ENSE00004023145	155261657	155261734
ENSE00004023212	155255981	155256419
ENSE00004023225	155256674	155256791
ENSE00004023226	155262086	155262360

Expression profiles

Bgee: expression breadth ubiquitous, 143 present calls, max score 96.80.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 77.3840 / max 343.6707, expressed in 1827 samples.

FANTOM5 promoters (10 alternative TSS)

Promoter ID	TPM avg	Samples expressed
14949	28.4686	1819
14948	25.4725	1806
14953	15.9916	1795
14951	1.8774	1256
14950	1.8341	1274
14952	1.3632	959
14947	1.3394	886
14946	0.7665	424
14945	0.2324	93
14954	0.0381	12

Top tissues by expression

143 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right adrenal gland cortex	UBERON:0035827	96.80	gold quality
right adrenal gland	UBERON:0001233	96.70	gold quality
left adrenal gland	UBERON:0001234	96.42	gold quality
left adrenal gland cortex	UBERON:0035825	96.32	gold quality
stromal cell of endometrium	CL:0002255	96.22	gold quality
apex of heart	UBERON:0002098	96.07	gold quality
heart left ventricle	UBERON:0002084	96.02	gold quality
skin of leg	UBERON:0001511	95.88	gold quality
mucosa of transverse colon	UBERON:0004991	95.81	gold quality
zone of skin	UBERON:0000014	95.79	gold quality
skin of abdomen	UBERON:0001416	95.78	gold quality
gastrocnemius	UBERON:0001388	95.51	gold quality
granulocyte	CL:0000094	95.47	gold quality
metanephros cortex	UBERON:0010533	95.44	gold quality
adrenal gland	UBERON:0002369	95.42	gold quality
lower esophagus mucosa	UBERON:0035834	95.32	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	95.16	gold quality
muscle of leg	UBERON:0001383	95.14	gold quality
skeletal muscle organ	UBERON:0014892	95.09	gold quality
spleen	UBERON:0002106	95.07	gold quality
hindlimb stylopod muscle	UBERON:0004252	95.02	gold quality
body of pancreas	UBERON:0001150	94.99	gold quality
right atrium auricular region	UBERON:0006631	94.97	gold quality
placenta	UBERON:0001987	94.91	gold quality
body of stomach	UBERON:0001161	94.87	gold quality
cortex of kidney	UBERON:0001225	94.84	gold quality
heart	UBERON:0000948	94.80	gold quality
lymph node	UBERON:0000029	94.76	gold quality
right lobe of thyroid gland	UBERON:0001119	94.63	gold quality
adult mammalian kidney	UBERON:0000082	94.60	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

28 targeting SCAMP3, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-27A-3P	99.98	72.13	2955
HSA-MIR-27B-3P	99.98	72.13	2955
HSA-MIR-9985	99.98	72.11	2939
HSA-MIR-128-3P	99.95	71.17	2484
HSA-MIR-216A-3P	99.95	71.19	2505
HSA-MIR-145-5P	99.92	71.13	1836
HSA-MIR-5195-3P	99.92	70.92	1877
HSA-MIR-3681-3P	99.88	70.46	2254
HSA-MIR-370-5P	99.78	66.81	706
HSA-MIR-6134	99.63	65.68	1537
HSA-MIR-12122	99.56	69.33	1672
HSA-MIR-584-3P	99.35	67.69	1082
HSA-MIR-4291	99.20	68.88	2969
HSA-MIR-922	99.02	67.23	1838
HSA-MIR-6895-3P	98.79	65.69	996
HSA-MIR-4274	98.59	66.10	630
HSA-MIR-6887-5P	98.56	68.49	1295
HSA-MIR-6795-5P	98.52	68.51	1277
HSA-MIR-3187-3P	97.38	65.80	904
HSA-MIR-7106-3P	97.33	65.33	644
HSA-MIR-6736-3P	96.98	65.22	1342
HSA-MIR-132-5P	96.61	65.79	115
HSA-MIR-7108-5P	96.42	66.17	598
HSA-MIR-644A	96.02	66.52	786
HSA-MIR-920	95.97	63.95	811
HSA-MIR-6726-5P	95.97	63.72	841
HSA-MIR-4300	95.85	64.56	1003
HSA-MIR-5591-5P	95.85	64.76	1002

Literature-anchored findings (GeneRIF, showing 8)

• Depletion of SCAMP3 in HeLa cells by inhibitory RNA accelerated degradation of epidermal growth factor receptor (EGFR) and EGF while inhibiting recycling (PMID:19158374)
• SCAMP3 tubules reflect a manipulation of specific post-Golgi trafficking that might allow Salmonella to acquire nutrients and membrane, or to control host immune responses. (PMID:19438519)
• SCAMP3 has a positive role in sorting into and budding of intralumenal vesicles and thereby controls the process of multivesicular endosome biogenesis. (PMID:21951651)
• SCAMP 3 is a novel regulator of endosomal morphology and composition. (PMID:27507217)
• The novel association of several endocytosis-associated proteins has been demonstrated, in particular, of CLTC and SCAMP3 with hepatocellular carcinoma progression. (PMID:27519163)
• MicroRNA-27a/b-3p and PPARG regulate SCAMP3 through a feed-forward loop during adipogenesis. (PMID:31554889)
• Secretory Carrier Membrane Protein 3 Interacts with 3A Viral Protein of Enterovirus and Participates in Viral Replication. (PMID:34378951)
• the SCAMPs may largely function at the same sites during vesicular transport rather than in separate post-Golgi recycling pathways. (PMID:9378760)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	scamp3	ENSDARG00000016765
mus_musculus	Scamp3	ENSMUSG00000028049
rattus_norvegicus	Scamp3	ENSRNOG00000020500
drosophila_melanogaster	Scamp	FBGN0040285
caenorhabditis_elegans	scm-1	WBGENE00004743

Paralogs (4): SCAMP1 (ENSG00000085365), SCAMP2 (ENSG00000140497), SCAMP5 (ENSG00000198794), SCAMP4 (ENSG00000227500)

Protein

Protein identifiers

Secretory carrier-associated membrane protein 3 — O14828 (reviewed: O14828)

All UniProt accessions (13): O14828, A0AAQ5BHL7, A0AAQ5BHL8, A0AAQ5BHM9, A0AAQ5BHP1, A0AAQ5BHP8, A0AAQ5BHQ3, A0AAQ5BHQ9, A0AAQ5BHR3, A0AAQ5BHR7, A0AAQ5BHR8, A0AAQ5BHS3, A0AAQ5BHV0

UniProt curated annotations — full annotation on UniProt →

Function. Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.

Subunit / interactions. Interacts with NEDD4, NEDD4L and TSG101. Interacts with RNF126.

Subcellular location. Membrane.

Tissue specificity. Widely expressed, with highest expression in heart and skeletal muscle.

Post-translational modifications. Monoubiquitinated.

Similarity. Belongs to the SCAMP family.

Isoforms (2)

UniProt ID	Names	Canonical?
O14828-1	1	yes
O14828-2	2

RefSeq proteins (2): NP_005689, NP_443069 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007273	SCAMP	Family

Pfam: PF04144

UniProt features (27 total): modified residue 8, sequence variant 4, transmembrane region 4, sequence conflict 3, topological domain 2, chain 1, cross-link 1, splice variant 1, mutagenesis site 1, region of interest 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O14828-F1	76.99	0.44

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (9): 37, 41, 53, 72, 76, 83, 85, 313, 32

Mutagenesis-validated functional residues (1):

Position	Phenotype
67	abolishes interaction with tsg101.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 165 (showing top): BORCZUK_MALIGNANT_MESOTHELIOMA_UP, ENK_UV_RESPONSE_KERATINOCYTE_UP, CHIANG_LIVER_CANCER_SUBCLASS_UNANNOTATED_DN, MORF_HDAC1, GOBP_VESICLE_MEDIATED_TRANSPORT, PATIL_LIVER_CANCER, TCF4_Q5, GOBP_EXOCYTOSIS, GOCC_TRANS_GOLGI_NETWORK, BLALOCK_ALZHEIMERS_DISEASE_UP, HNF4_DR1_Q3, MORF_BUB3, GOBP_SECRETION, chr1q22, BOYAULT_LIVER_CANCER_SUBCLASS_G12_UP

GO Biological Process (3): exocytosis (GO:0006887), post-Golgi vesicle-mediated transport (GO:0006892), protein transport (GO:0015031)

GO Molecular Function (1): ubiquitin protein ligase binding (GO:0031625)

GO Cellular Component (4): trans-Golgi network membrane (GO:0032588), recycling endosome membrane (GO:0055038), extracellular exosome (GO:0070062), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
vesicle-mediated transport	1
secretion by cell	1
vesicle fusion to plasma membrane	1
Golgi vesicle transport	1
transport	1
intracellular protein localization	1
establishment of protein localization	1
ubiquitin-like protein ligase binding	1
trans-Golgi network	1
organelle membrane	1
endosome membrane	1
recycling endosome	1
extracellular vesicle	1
cellular anatomical structure	1

Protein interactions and networks

STRING

920 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
SCAMP3	TSG101	Q99816	728
SCAMP3	DRAM1	Q8N682	696
SCAMP3	ENTREP3	P81408	489
SCAMP3	KRTCAP2	Q8N6L1	455
SCAMP3	STAM2	O75886	420
SCAMP3	ERBB3	P21860	399
SCAMP3	SCCPDH	Q8NBX0	399
SCAMP3	MUC1	P13931	396
SCAMP3	MTX1	Q13505	387
SCAMP3	HCN3	Q9P1Z3	379
SCAMP3	TRIM46	Q7Z4K8	371
SCAMP3	STAM	Q92783	370
SCAMP3	CMC1	Q7Z7K0	361
SCAMP3	CRNN	Q9UBG3	350
SCAMP3	TMX4	Q9H1E5	349

IntAct

195 interactions, top by confidence:

A	B	Type	Score
IFT70B	IFT56	psi-mi:“MI:0914”(association)	0.790
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
TRDN	TMEM223	psi-mi:“MI:0914”(association)	0.640
CD27	TCAF2	psi-mi:“MI:0914”(association)	0.640
FAM234B	ABCD4	psi-mi:“MI:0914”(association)	0.620
SLC39A4	TMEM120B	psi-mi:“MI:0914”(association)	0.530
VAMP5	NBAS	psi-mi:“MI:0914”(association)	0.530
TMEM63A	AP3D1	psi-mi:“MI:0914”(association)	0.530
SCAMP2	SCAMP3	psi-mi:“MI:0914”(association)	0.530
DLK1	SCAMP3	psi-mi:“MI:0914”(association)	0.530
NRAS	ESYT2	psi-mi:“MI:2364”(proximity)	0.480
vpu	SCAMP3	psi-mi:“MI:0914”(association)	0.460
NEDD4	SCAMP3	psi-mi:“MI:0407”(direct interaction)	0.440
YAP1	SCAMP3	psi-mi:“MI:0407”(direct interaction)	0.440
SCAMP3	PCNA	psi-mi:“MI:0915”(physical association)	0.370
Tor1aip1	PEX10	psi-mi:“MI:0914”(association)	0.350
Dync1li1	SSR3	psi-mi:“MI:0914”(association)	0.350
Cdc16	ANAPC15	psi-mi:“MI:0914”(association)	0.350
Cdc26		psi-mi:“MI:0914”(association)	0.350
CDC73	SCAMP3	psi-mi:“MI:0914”(association)	0.350
Pcgf1	SCAMP3	psi-mi:“MI:0914”(association)	0.350
Ercc6l	RPL17	psi-mi:“MI:0914”(association)	0.350
NCSTN	ESYT2	psi-mi:“MI:0914”(association)	0.350
Ncstn	DERL1	psi-mi:“MI:0914”(association)	0.350

BioGRID (319): SCAMP3 (Affinity Capture-MS), SCAMP3 (Affinity Capture-MS), BCAP31 (Co-fractionation), DDOST (Co-fractionation), SCAMP3 (Co-fractionation), SCAMP3 (Co-fractionation), SCAMP3 (Co-fractionation), SCAMP3 (Affinity Capture-MS), SCAMP3 (Proximity Label-MS), SCAMP3 (Affinity Capture-MS), SCAMP3 (Affinity Capture-MS), SCAMP3 (Affinity Capture-MS), SCAMP3 (Affinity Capture-MS), SCAMP3 (Affinity Capture-MS), SCAMP3 (Affinity Capture-MS)

ESM2 similar proteins: A2YMP7, A4RE85, B2WCU2, B3M9W1, B3NDM7, B4GRI8, B4HIJ8, B4J043, B4L0H1, B4LIH0, B4MXW6, B4PD01, B4QLP9, O14828, O35609, O95070, P87148, Q0JAI9, Q0UUE1, Q1E5A9, Q29BL9, Q3T196, Q4FZQ0, Q58DR5, Q5APC0, Q5BJH7, Q5RBL0, Q5U3G6, Q60EA5, Q6GN58, Q6P301, Q6P6G5, Q6PC24, Q6Z8F5, Q75IC7, Q7F613, Q7SDN3, Q8H5X5, Q8MRQ4, Q91VU1

Diamond homologs: O14828, O15126, O15127, O35609, O77735, P56603, Q17QF8, Q28F21, Q58DF6, Q58DR5, Q5R5Z8, Q5RCY9, Q6GM42, Q6GPA8, Q6P0C7, Q8K021, Q8TAC9, Q969E2, Q9ERN0, Q9ET20, Q9JKD3, Q9JKE3, Q9JKV5, Q9LR68, Q0JAI9, Q6Z8F5, Q9SXA5, Q9SKT3, Q9M5P2, Q9C6X2, Q9ZTX0, Q60EA5

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
EGFR	unknown	SCAMP3	phosphorylation
EGFR	“up-regulates activity”	SCAMP3	phosphorylation

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 211 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Neutrophil degranulation	18	3.2×	2e-03

GO biological processes:

GO term	Partners	Fold	FDR
regulation of long-term neuronal synaptic plasticity	5	29.9×	7e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

71 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	57
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1191 predictions. Top by Δscore:

Variant	Effect	Δscore
1:155256415:TGGAT:T	acceptor_gain	1.0000
1:155256416:GGAT:G	acceptor_gain	1.0000
1:155256417:GAT:G	acceptor_gain	1.0000
1:155256420:C:CC	acceptor_gain	1.0000
1:155256420:C:T	acceptor_loss	1.0000
1:155256421:T:G	acceptor_loss	1.0000
1:155256672:AC:A	donor_gain	1.0000
1:155256673:CC:C	donor_gain	1.0000
1:155256692:T:TA	donor_gain	1.0000
1:155256787:AGCCA:A	acceptor_gain	1.0000
1:155256788:GCCA:G	acceptor_gain	1.0000
1:155256789:CCA:C	acceptor_gain	1.0000
1:155256789:CCAC:C	acceptor_gain	1.0000
1:155256790:CA:C	acceptor_gain	1.0000
1:155256790:CAC:C	acceptor_gain	1.0000
1:155256792:C:CC	acceptor_gain	1.0000
1:155256792:CTGT:C	acceptor_loss	1.0000
1:155256794:G:GC	acceptor_gain	1.0000
1:155257333:T:TA	donor_gain	1.0000
1:155257654:CTGC:C	acceptor_gain	1.0000
1:155257657:CCTAA:C	acceptor_loss	1.0000
1:155257658:CTAA:C	acceptor_loss	1.0000
1:155258824:A:AC	donor_gain	1.0000
1:155258825:C:CC	donor_gain	1.0000
1:155258825:CA:C	donor_gain	1.0000
1:155258825:CACAT:C	donor_gain	1.0000
1:155260324:A:AC	donor_gain	1.0000
1:155260325:C:CC	donor_gain	1.0000
1:155260325:CTTA:C	donor_gain	1.0000
1:155260326:TTA:T	donor_loss	1.0000

AlphaMissense

2252 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:155260390:C:G	A110P	0.999
1:155260401:A:G	L106P	0.999
1:155256322:G:T	A332D	0.998
1:155256787:A:G	W262R	0.998
1:155256787:A:T	W262R	0.998
1:155257632:G:C	N181K	0.998
1:155257632:G:T	N181K	0.998
1:155258940:A:G	W135R	0.998
1:155258940:A:T	W135R	0.998
1:155256376:G:T	A314D	0.997
1:155257356:G:C	F236L	0.997
1:155257356:G:T	F236L	0.997
1:155257358:A:G	F236L	0.997
1:155257526:A:G	W217R	0.997
1:155257526:A:T	W217R	0.997
1:155257529:A:G	C216R	0.997
1:155257541:A:G	C212R	0.997
1:155258938:C:A	W135C	0.997
1:155258938:C:G	W135C	0.997
1:155256364:A:G	F318S	0.996
1:155256387:G:C	S310R	0.996
1:155256387:G:T	S310R	0.996
1:155256389:T:G	S310R	0.996
1:155256403:C:G	R305P	0.996
1:155257498:C:G	R226P	0.996
1:155258832:A:G	W171R	0.996
1:155258832:A:T	W171R	0.996
1:155260391:C:A	K109N	0.996
1:155260391:C:G	K109N	0.996
1:155260395:C:G	R108P	0.996

dbSNP variants (sampled 300 via entrez): RS1000115651 (1:155259949 G>A,C), RS1000529703 (1:155260122 TTGTG>T,TTG), RS1001015417 (1:155255542 G>A), RS1001517739 (1:155263713 AGAATCCAG>A), RS1002216533 (1:155262872 G>A), RS1002293618 (1:155256154 A>T), RS1002406489 (1:155255894 C>A), RS1002924414 (1:155262397 C>A,T), RS1003413249 (1:155257147 G>A,C), RS1003521261 (1:155260964 G>C), RS1004219344 (1:155259815 C>T), RS1004551167 (1:155257761 G>A), RS1005195769 (1:155261916 G>A), RS1005634545 (1:155261628 T>A), RS1006021983 (1:155259159 A>C,G,T)

Disease associations

OMIM: gene MIM:606913 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

18 associations (top):

Study	Trait	p-value
GCST000879_41	Crohn’s disease	2.000000e-13
GCST004131_70	Inflammatory bowel disease	6.000000e-08
GCST004132_44	Crohn’s disease	2.000000e-07
GCST007294_124	Body fat distribution (trunk fat ratio)	8.000000e-35
GCST007294_3	Body fat distribution (trunk fat ratio)	6.000000e-21
GCST007294_50	Body fat distribution (trunk fat ratio)	1.000000e-15
GCST007295_17	Body fat distribution (leg fat ratio)	3.000000e-13
GCST007295_37	Body fat distribution (leg fat ratio)	7.000000e-17
GCST007295_72	Body fat distribution (leg fat ratio)	1.000000e-28
GCST010696_19	Cortical thickness (min-P)	2.000000e-10
GCST010697_10	Cortical surface area (min-P)	3.000000e-10
GCST010698_59	Subcortical volume (min-P)	9.000000e-10
GCST010699_20	Brain morphology (min-P)	7.000000e-10
GCST010700_5	Cortical thickness (MOSTest)	8.000000e-17
GCST010701_66	Cortical surface area (MOSTest)	1.000000e-09
GCST010702_43	Subcortical volume (MOSTest)	3.000000e-10
GCST010703_253	Brain morphology (MOSTest)	4.000000e-14
GCST90002404_428	Red cell distribution width	5.000000e-17

EFO canonical traits (4, from GWAS)

EFO ID	Trait name
EFO:0004341	body fat distribution
EFO:0004346	neuroimaging measurement
EFO:0004840	cortical thickness
EFO:0009188	Red cell distribution width

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6066432 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.54	Kd	2.915	nM	CHEMBL3752910
8.54	ED50	2.915	nM	CHEMBL3752910
6.00	Kd	1012	nM	CHEMBL5653589
6.00	ED50	1012	nM	CHEMBL5653589

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149348: Binding affinity to human SCAMP3 incubated for 45 mins by Kinobead based pull down assay	kd	0.0029	uM
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149348: Binding affinity to human SCAMP3 incubated for 45 mins by Kinobead based pull down assay	kd	1.0119	uM

CTD chemical–gene interactions

31 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, affects cotreatment, increases expression	3
FR900359	affects phosphorylation	1
bisphenol F	increases expression	1
bufotalin	increases expression	1
alpha-pinene	increases expression, increases abundance, affects cotreatment	1
methacrylaldehyde	affects cotreatment, increases expression, increases abundance	1
di-n-butylphosphoric acid	affects expression	1
perfluorooctane sulfonic acid	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
bisphenol S	increases expression	1
LDN 193189	affects cotreatment, decreases expression	1
bisphenol AF	increases expression	1
Irinotecan	affects cotreatment, decreases expression	1
Sunitinib	increases expression	1
Acrolein	affects cotreatment, increases expression, increases abundance	1
Air Pollutants	affects cotreatment, increases abundance, increases expression	1
Benzo(a)pyrene	increases methylation	1
Caffeine	decreases phosphorylation	1
Fluorouracil	affects cotreatment, decreases expression	1
Ivermectin	decreases expression	1
Ozone	affects cotreatment, increases expression, increases abundance	1
Phenolsulfonphthalein	affects cotreatment, increases expression	1
Polycyclic Aromatic Hydrocarbons	affects expression	1
Selenium	affects cotreatment, increases expression	1
Smoke	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Valproic Acid	affects expression	1
Vitamin E	affects cotreatment, increases expression	1
Aflatoxin B1	increases methylation	1
Volatile Organic Compounds	affects cotreatment, increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652390	Binding	Binding affinity to human SCAMP3 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.