SDF4

Summary

SDF4 (stromal cell derived factor 4, HGNC:24188) is a protein-coding gene on chromosome 1p36.33, encoding 45 kDa calcium-binding protein (Q9BRK5). May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

This gene encodes a stromal cell derived factor that is a member of the CREC protein family. The encoded protein contains six EF-hand motifs and calcium-binding motifs. This protein localizes to the Golgi lumen and may be involved in regulating calcium dependent cellular activities.

Source: NCBI Gene 51150 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 89 total
• Druggable target: yes
• MANE Select transcript: NM_016176

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24188
Approved symbol	SDF4
Name	stromal cell derived factor 4
Location	1p36.33
Locus type	gene with protein product
Status	Approved
Aliases	Cab45
Ensembl gene	ENSG00000078808
Ensembl biotype	protein_coding
OMIM	614282
Entrez	51150

Gene structure

Transcript identifiers

Ensembl transcripts: 21 — 17 protein_coding, 2 retained_intron, 1 protein_coding_CDS_not_defined, 1 nonsense_mediated_decay

ENST00000263741, ENST00000360001, ENST00000403997, ENST00000459994, ENST00000465727, ENST00000478938, ENST00000494748, ENST00000900948, ENST00000900949, ENST00000900950, ENST00000900951, ENST00000900952, ENST00000900953, ENST00000900954, ENST00000900955, ENST00000921530, ENST00000921531, ENST00000921532, ENST00000921533, ENST00000969251, ENST00000969252

RefSeq mRNA: 2 — MANE Select: NM_016176 NM_016176, NM_016547

CCDS: CCDS12, CCDS30553

Canonical transcript exons

ENST00000360001 — 7 exons

Exon	Start	End
ENSE00000869639	1223244	1223357
ENSE00000869640	1223832	1223968
ENSE00001131112	1218769	1218927
ENSE00001480105	1216931	1217688
ENSE00001945650	1231892	1232001
ENSE00003459646	1228468	1228946
ENSE00003628628	1218458	1218633

Expression profiles

Bgee: expression breadth ubiquitous, 279 present calls, max score 99.24.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 99.8581 / max 796.8220, expressed in 1825 samples.

FANTOM5 promoters (8 alternative TSS)

Promoter ID	TPM avg	Samples expressed
9734	80.3108	1825
9737	4.5528	1624
9736	4.3465	1640
9733	3.7074	1578
201310	3.6870	1589
9735	2.3581	1335
9732	0.5873	312
9731	0.3081	132

Top tissues by expression

291 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
stromal cell of endometrium	CL:0002255	99.24	gold quality
right hemisphere of cerebellum	UBERON:0014890	98.27	gold quality
cartilage tissue	UBERON:0002418	98.05	gold quality
cerebellar hemisphere	UBERON:0002245	97.98	gold quality
cerebellar cortex	UBERON:0002129	97.96	gold quality
mucosa of transverse colon	UBERON:0004991	97.94	gold quality
thoracic aorta	UBERON:0001515	97.91	gold quality
ascending aorta	UBERON:0001496	97.90	gold quality
descending thoracic aorta	UBERON:0002345	97.88	gold quality
cardia of stomach	UBERON:0001162	97.84	gold quality
right atrium auricular region	UBERON:0006631	97.84	gold quality
right coronary artery	UBERON:0001625	97.82	gold quality
cardiac atrium	UBERON:0002081	97.77	gold quality
apex of heart	UBERON:0002098	97.71	gold quality
ileal mucosa	UBERON:0000331	97.69	gold quality
cerebellum	UBERON:0002037	97.58	gold quality
transverse colon	UBERON:0001157	97.50	gold quality
body of stomach	UBERON:0001161	97.49	gold quality
trachea	UBERON:0003126	97.44	gold quality
pylorus	UBERON:0001166	97.43	gold quality
aorta	UBERON:0000947	97.41	gold quality
small intestine Peyer’s patch	UBERON:0003454	97.37	gold quality
coronary artery	UBERON:0001621	97.33	gold quality
nipple	UBERON:0002030	97.33	gold quality
fundus of stomach	UBERON:0001160	97.31	gold quality
parotid gland	UBERON:0001831	97.31	gold quality
left coronary artery	UBERON:0001626	97.30	gold quality
endocervix	UBERON:0000458	97.29	gold quality
small intestine	UBERON:0002108	97.28	gold quality
right ovary	UBERON:0002118	97.26	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

34 targeting SDF4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-559	99.95	72.28	3609
HSA-MIR-548AB	99.95	71.31	3488
HSA-MIR-548A-5P	99.94	71.27	3482
HSA-MIR-548AD-5P	99.94	71.23	3502
HSA-MIR-548AE-5P	99.94	71.23	3502
HSA-MIR-548AK	99.94	71.24	3488
HSA-MIR-548AM-5P	99.94	71.24	3488
HSA-MIR-548AP-5P	99.94	71.14	3489
HSA-MIR-548AQ-5P	99.94	71.34	3426
HSA-MIR-548AR-5P	99.94	71.28	3515
HSA-MIR-548AS-5P	99.94	71.22	3482
HSA-MIR-548AU-5P	99.94	71.24	3488
HSA-MIR-548AY-5P	99.94	71.23	3502
HSA-MIR-548B-5P	99.94	71.23	3502
HSA-MIR-548BB-5P	99.94	71.27	3509
HSA-MIR-548C-5P	99.94	71.24	3488
HSA-MIR-548D-5P	99.94	71.23	3502
HSA-MIR-548H-5P	99.94	71.24	3488
HSA-MIR-548I	99.94	71.25	3481
HSA-MIR-548J-5P	99.94	71.14	3489
HSA-MIR-548O-5P	99.94	71.24	3488
HSA-MIR-548W	99.94	71.24	3488
HSA-MIR-548Y	99.94	71.28	3514
HSA-MIR-1305	99.91	71.43	3443
HSA-MIR-765	99.84	68.24	2442
HSA-MIR-498-5P	99.76	69.64	1807
HSA-MIR-11181-3P	99.75	66.38	2205
HSA-MIR-6512-3P	99.65	66.07	1468
HSA-MIR-6720-5P	99.65	66.22	1459
HSA-MIR-5003-5P	99.61	69.13	1624

Literature-anchored findings (GeneRIF, showing 11)

• In a yeast two-hybrid screen the EF-hand Ca(2+)-binding protein Cab45 was identified as an interaction partner of Munc18b. (PMID:17442889)
• SDF-2, SDF-4 and SDF-5 are expressed in mammary tissues and cells and that a reduced level of SDF-2 and SDF-4 are associated with a poor clinical outcome. (PMID:19513569)
• Calcium is required for the retention of Cab45 in the Golgi membranes, where Cab45 binds secretory cargo in a calcium-dependent reaction. (PMID:23266954)
• Cab45S, a novel regulator of GRP78/BiP, suppresses endoplasmic reticulum stress-induced IRE1 activation and apoptosis by binding to and elevating GRP78/BiP, and has a role in the inhibition of ER stress-induced apoptosis. (PMID:24810055)
• The obtained results indicate that the changes in gene expression in bone marrow progenitor cells can be involved into space flight-induced osteopenia. (PMID:25509878)
• Data reveal that the ability of Cab45S to inhibit SERCA2b activity is crucial for its role as a modulator of cell proliferation and tumor growth. (PMID:25772237)
• Ca(2+)-dependent changes in Cab45 mediate sorting of specific cargo molecules at the trans-Golgi network. (PMID:27138253)
• Fam20C regulates protein secretion by Cab45 phosphorylation. (PMID:32422653)
• SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis via Negatively Regulating ER Stress. (PMID:34326834)
• Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning. (PMID:36398980)
• LINC00173 facilitates tumor progression by stimulating RAB1B-mediated PA2G4 and SDF4 secretion in nasopharyngeal carcinoma. (PMID:36606322)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	sdf4	ENSDARG00000070682
mus_musculus	Sdf4	ENSMUSG00000029076
rattus_norvegicus	Sdf4	ENSRNOG00000019981
drosophila_melanogaster	myd	FBGN0051475
caenorhabditis_elegans		WBGENE00016965

Paralogs (4): RCN1 (ENSG00000049449), RCN2 (ENSG00000117906), CALU (ENSG00000128595), RCN3 (ENSG00000142552)

Protein

Protein identifiers

45 kDa calcium-binding protein — Q9BRK5 (reviewed: Q9BRK5)

Alternative names: Stromal cell-derived factor 4

All UniProt accessions (4): A0A5F9UJX7, A0A5F9UP49, G3V1E2, H0Y3T6

UniProt curated annotations — full annotation on UniProt →

Function. May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.

Subunit / interactions. Isoform 5 interacts with STXBP1; the interaction is enhanced in presence of calcium. Isoform 5 interacts with STX3.

Subcellular location. Golgi apparatus lumen Cytoplasm. Cell membrane. Cell projection. Bleb.

Tissue specificity. Ubiquitous. Isoform 5 is expressed in pancreas.

Domain organisation. Binds calcium via its EF-hands. Isoform 5 binds calcium.

Similarity. Belongs to the CREC family.

Isoforms (6)

UniProt ID	Names	Canonical?
Q9BRK5-1	1, Cab45a, Cab45-G	yes
Q9BRK5-2	2
Q9BRK5-3	3
Q9BRK5-4	4
Q9BRK5-5	5, Cab45b, Cab45-C
Q9BRK5-6	6

RefSeq proteins (2): NP_057260, NP_057631 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002048	EF_hand_dom	Domain
IPR011992	EF-hand-dom_pair	Homologous_superfamily
IPR018247	EF_Hand_1_Ca_BS	Binding_site
IPR027240	CAB45_EFh	Domain

Pfam: PF13202, PF13499

UniProt features (69 total): binding site 26, sequence conflict 16, splice variant 7, domain 6, modified residue 5, mutagenesis site 3, sequence variant 2, signal peptide 1, chain 1, glycosylation site 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BRK5-F1	81.03	0.60

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (26): 113; 115; 117; 122; 150; 152; 154; 156; 161; 213; 220; 246 …

Post-translational modifications (5): 99, 193, 217, 265, 299

Glycosylation sites (1): 40

Mutagenesis-validated functional residues (3):

Position	Phenotype
257	does not affect calcium-binding.
302	inhibits calcium-binding.
338	does not affect calcium-binding.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 175 (showing top): GOBP_HINDBRAIN_DEVELOPMENT, GOBP_RESPONSE_TO_ETHANOL, GOBP_METENCEPHALON_DEVELOPMENT, GOBP_CELLULAR_COMPONENT_MAINTENANCE, GOBP_VESICLE_MEDIATED_TRANSPORT, GOBP_UV_PROTECTION, GOBP_EXOCYTOSIS, GOBP_CELL_JUNCTION_ORGANIZATION, GOBP_RESPONSE_TO_OXYGEN_CONTAINING_COMPOUND, GOBP_RESPONSE_TO_UV, GOBP_SECRETION, GOBP_RESPONSE_TO_RADIATION, GOBP_CALCIUM_ION_REGULATED_EXOCYTOSIS, GOBP_FAT_CELL_DIFFERENTIATION, GOBP_RESPONSE_TO_ABIOTIC_STIMULUS

GO Biological Process (7): UV protection (GO:0009650), calcium-ion regulated exocytosis (GO:0017156), cerebellum development (GO:0021549), fat cell differentiation (GO:0045444), response to ethanol (GO:0045471), zymogen granule exocytosis (GO:0070625), maintenance of synapse structure (GO:0099558)

GO Molecular Function (3): calcium ion binding (GO:0005509), identical protein binding (GO:0042802), metal ion binding (GO:0046872)

GO Cellular Component (9): cytoplasm (GO:0005737), late endosome (GO:0005770), endoplasmic reticulum (GO:0005783), Golgi lumen (GO:0005796), plasma membrane (GO:0005886), membrane (GO:0016020), bleb (GO:0032059), extracellular exosome (GO:0070062), Golgi apparatus (GO:0005794)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
cytoplasm	2
endomembrane system	2
intracellular membrane-bounded organelle	2
response to UV	1
regulated exocytosis	1
metencephalon development	1
anatomical structure development	1
cell differentiation	1
response to alcohol	1
calcium-ion regulated exocytosis	1
cell junction maintenance	1
synapse organization	1
metal ion binding	1
protein binding	1
cation binding	1
intracellular anatomical structure	1
endosome	1
Golgi apparatus	1
intracellular organelle lumen	1
membrane	1
cell periphery	1
plasma membrane bounded cell projection	1
extracellular vesicle	1

Protein interactions and networks

STRING

1520 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
SDF4	S100B	P04271	937
SDF4	CALB1	P05937	884
SDF4	PVALB	P20472	845
SDF4	CALM1	P02593	834
SDF4	CALML4	Q96GE6	833
SDF4	CALML3	P27482	832
SDF4	CALML5	Q9NZT1	832
SDF4	CALML6	Q8TD86	823
SDF4	CALB2	P22676	801
SDF4	S100G	P29377	792
SDF4	S100A4	P26447	722
SDF4	LYZL1	Q6UWQ5	721
SDF4	S100A6	P06703	720
SDF4	LYZ	P00695	690
SDF4	CASQ1	P31415	658

IntAct

126 interactions, top by confidence:

A	B	Type	Score
IFT27	IFT56	psi-mi:“MI:0914”(association)	0.690
SDF4	ACAD11	psi-mi:“MI:0914”(association)	0.640
USP12	PHLPP1	psi-mi:“MI:0914”(association)	0.570
CASP6	SDF4	psi-mi:“MI:0915”(physical association)	0.560
SDF4	LAMP2	psi-mi:“MI:0915”(physical association)	0.560
SDF4	SH3GLB1	psi-mi:“MI:0915”(physical association)	0.560
PRPF40A	SDF4	psi-mi:“MI:0915”(physical association)	0.560
ILK	HAX1	psi-mi:“MI:0914”(association)	0.530
YWHAZ	BLTP3B	psi-mi:“MI:0914”(association)	0.530
SDF4	GTPBP6	psi-mi:“MI:0914”(association)	0.530
ILK	ILVBL	psi-mi:“MI:0914”(association)	0.530
ASCC1	TRIP4	psi-mi:“MI:0914”(association)	0.530
env	PSMD11	psi-mi:“MI:0914”(association)	0.460
GPC1	SNAP23	psi-mi:“MI:0915”(physical association)	0.400
GPC1	GANAB	psi-mi:“MI:0915”(physical association)	0.400
MOK	SDF4	psi-mi:“MI:0915”(physical association)	0.400
SDF4	RPA2	psi-mi:“MI:0915”(physical association)	0.370
SDF4	NR1I2	psi-mi:“MI:0915”(physical association)	0.370

BioGRID (245): SDF4 (Affinity Capture-MS), SDF4 (Affinity Capture-MS), SDF4 (Affinity Capture-MS), SDF4 (Affinity Capture-MS), ACAD11 (Affinity Capture-MS), IQGAP2 (Affinity Capture-MS), MYO9B (Affinity Capture-MS), GPRIN1 (Affinity Capture-MS), ABCB7 (Affinity Capture-MS), SMC1A (Affinity Capture-MS), SMC3 (Affinity Capture-MS), RICTOR (Affinity Capture-MS), GTPBP6 (Affinity Capture-MS), LRFN1 (Affinity Capture-MS), RGPD5 (Affinity Capture-MS)

ESM2 similar proteins: A0A1S4GYH9, A0A7J6K452, A5YVD9, A8XEZ1, A9LKE4, A9LKF6, B9FS74, D0Z5N4, E2AX35, H2KZB2, H2KZH5, O02217, O55159, P02592, P07164, P14725, P16422, P25027, P34542, P39047, P39931, P41045, P41048, P41049, P85831, Q06BR2, Q08121, Q08629, Q10004, Q1WER1, Q3HRN7, Q3T0L5, Q3ZBZ1, Q4R585, Q5BKL9, Q5EP01, Q5F381, Q5ZKE5, Q61112, Q62288

Diamond homologs: A5YVD9, Q3ZBZ1, Q4R585, Q5BKL9, Q5ZKE5, Q61112, Q66JA6, Q7ZUC2, Q91ZS3, Q9BRK5, J3S9D9, B5X186

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
FAM20C	“up-regulates activity”	SDF4	phosphorylation

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 134 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

GO biological processes:

GO term	Partners	Fold	FDR
G1/S transition of mitotic cell cycle	7	12.7×	1e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

89 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	69
Likely benign	5
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

1875 predictions. Top by Δscore:

Variant	Effect	Δscore
1:1217684:TAGCT:T	acceptor_gain	1.0000
1:1217685:AGCT:A	acceptor_gain	1.0000
1:1217686:GCTCT:G	acceptor_loss	1.0000
1:1217687:CT:C	acceptor_gain	1.0000
1:1217688:TCTGC:T	acceptor_loss	1.0000
1:1217689:C:CC	acceptor_gain	1.0000
1:1217689:CTGCG:C	acceptor_loss	1.0000
1:1217690:T:C	acceptor_loss	1.0000
1:1218452:GCTCA:G	donor_loss	1.0000
1:1218453:CTCA:C	donor_loss	1.0000
1:1218457:C:CT	donor_loss	1.0000
1:1218634:C:CC	acceptor_gain	1.0000
1:1218727:T:TA	donor_gain	1.0000
1:1223242:A:AC	donor_gain	1.0000
1:1223242:ACTTT:A	donor_gain	1.0000
1:1223243:C:CC	donor_gain	1.0000
1:1223243:CTTT:C	donor_gain	1.0000
1:1223243:CTTTC:C	donor_gain	1.0000
1:1223246:T:A	donor_gain	1.0000
1:1223826:CAGTA:C	donor_loss	1.0000
1:1223828:GTA:G	donor_loss	1.0000
1:1223829:TACC:T	donor_loss	1.0000
1:1223830:A:AG	donor_loss	1.0000
1:1223830:ACCGT:A	donor_gain	1.0000
1:1223831:C:CA	donor_loss	1.0000
1:1223831:CCGT:C	donor_gain	1.0000
1:1223831:CCGTC:C	donor_gain	1.0000
1:1223834:T:TA	donor_gain	1.0000
1:1223835:C:A	donor_gain	1.0000
1:1223873:T:A	donor_gain	1.0000

AlphaMissense

2400 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:1217646:C:G	A319P	0.999
1:1218512:A:C	F286L	0.998
1:1218512:A:T	F286L	0.998
1:1218514:A:G	F286L	0.998
1:1218596:G:C	F258L	0.998
1:1218596:G:T	F258L	0.998
1:1218598:A:G	F258L	0.998
1:1218895:A:G	W204R	0.998
1:1218895:A:T	W204R	0.998
1:1217624:G:T	A326D	0.997
1:1218597:A:C	F258C	0.997
1:1218597:A:G	F258S	0.997
1:1223344:C:A	W159C	0.997
1:1223344:C:G	W159C	0.997
1:1217515:A:C	F362L	0.996
1:1217515:A:T	F362L	0.996
1:1217517:A:G	F362L	0.996
1:1217625:C:G	A326P	0.996
1:1218477:A:T	V298E	0.996
1:1218771:A:G	L245P	0.996
1:1218893:C:A	W204C	0.996
1:1218893:C:G	W204C	0.996
1:1223346:A:G	W159R	0.996
1:1223346:A:T	W159R	0.996
1:1217564:A:G	F346S	0.995
1:1217600:A:G	L334P	0.995
1:1217645:G:T	A319D	0.995
1:1218462:A:G	L303P	0.995
1:1218513:A:G	F286S	0.995
1:1218524:T:A	R282S	0.995

dbSNP variants (sampled 300 via entrez): RS1000080973 (1:1223372 C>T), RS1000339484 (1:1219793 C>T), RS1000435325 (1:1223003 C>G,T), RS1000779379 (1:1231531 A>C), RS1000780878 (1:1219631 C>T), RS1000840121 (1:1222050 C>A), RS1000992685 (1:1225796 G>A,T), RS1001090982 (1:1229337 G>C), RS1001121938 (1:1229196 T>C,G), RS1001133130 (1:1217138 T>TC), RS1001317189 (1:1225981 C>T), RS1001477299 (1:1226405 C>A,T), RS1001491240 (1:1228381 C>A,T), RS1001635716 (1:1233580 C>G,T), RS1001910456 (1:1225748 A>G)

Disease associations

OMIM: gene MIM:614282 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST005537_122	Chronic inflammatory diseases (ankylosing spondylitis, Crohn’s disease, psoriasis, primary sclerosing cholangitis, ulcerative colitis) (pleiotropy)	2.000000e-10
GCST006988_199	Blond vs. brown/black hair color	2.000000e-09
GCST007932_105	Medication use (thyroid preparations)	3.000000e-08

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0003924	hair color
EFO:0009933	Thyroid preparation use measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL4742317 (PROTEIN-PROTEIN INTERACTION), CHEMBL6067531 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.29	Kd	518.7	nM	CHEMBL3752910
6.29	ED50	518.7	nM	CHEMBL3752910

PubChem BioAssay actives

1 with measured affinity, of 3 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149932: Binding affinity to human SDF4 incubated for 45 mins by Kinobead based pull down assay	kd	0.5187	uM

CTD chemical–gene interactions

27 total (human), top 27 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Air Pollutants	affects cotreatment, increases abundance, increases oxidation, affects expression	2
Ozone	affects cotreatment, increases oxidation, increases abundance, affects expression	2
Tobacco Smoke Pollution	affects expression, decreases methylation	2
Valproic Acid	increases methylation, increases expression	2
aristolochic acid I	increases expression	1
FR900359	affects phosphorylation	1
TAK-243	increases sumoylation	1
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
alpha-pinene	affects cotreatment, increases oxidation, increases abundance	1
bisphenol A	increases expression	1
salinomycin	decreases expression	1
sodium arsenite	increases expression	1
cobaltous chloride	increases expression	1
perfluorooctanoic acid	decreases expression	1
methacrylaldehyde	affects cotreatment, increases oxidation, increases abundance	1
ICG 001	decreases expression	1
bisphenol AF	increases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	decreases expression	1
Acrolein	affects cotreatment, increases oxidation, increases abundance	1
Ivermectin	decreases expression	1
Lead	decreases expression	1
Methotrexate	affects response to substance	1
Silicon Dioxide	decreases methylation	1
Smoke	decreases expression	1
Cadmium Chloride	decreases expression, increases expression, increases methylation	1
Volatile Organic Compounds	affects cotreatment, increases oxidation	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4713769	Binding	Protac activity at CRBN/SDF4 in human BxPC-3 cells assessed as SDF4 degradation incubated for 16 hrs by proteomic analysis	Discovery of a Napabucasin PROTAC as an Effective Degrader of the E3 Ligase ZFP91. — J Med Chem

Cellosaurus cell lines

2 cell lines: 1 transformed cell line, 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3GK	Abcam HEK293T SDF4 KO	Transformed cell line	Female
CVCL_E2JR	HAP1 SDF4 (-)	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.