SEC61G

Summary

SEC61G (SEC61 translocon subunit gamma, HGNC:18277) is a protein-coding gene on chromosome 7p11.2, encoding Protein transport protein Sec61 subunit gamma (P60059). Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). It is a common-essential gene (DepMap: required in 96.1% of cancer cell lines).

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the gamma-subunit protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Source: NCBI Gene 23480 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 8 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 96.1% of screened cell lines (common-essential)
• MANE Select transcript: NM_014302

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:18277
Approved symbol	SEC61G
Name	SEC61 translocon subunit gamma
Location	7p11.2
Locus type	gene with protein product
Status	Approved
Aliases	SSS1
Ensembl gene	ENSG00000132432
Ensembl biotype	protein_coding
OMIM	609215
Entrez	23480

Gene structure

Transcript identifiers

Ensembl transcripts: 24 — 21 protein_coding, 3 retained_intron

ENST00000352861, ENST00000395535, ENST00000415949, ENST00000450622, ENST00000460484, ENST00000461536, ENST00000480303, ENST00000873613, ENST00000873614, ENST00000873615, ENST00000873616, ENST00000916373, ENST00000916374, ENST00000916375, ENST00000916376, ENST00000916377, ENST00000916378, ENST00000916379, ENST00000916380, ENST00000916381, ENST00000916382, ENST00000916383, ENST00000916384, ENST00000946003

RefSeq mRNA: 2 — MANE Select: NM_014302 NM_001012456, NM_014302

CCDS: CCDS5513

Canonical transcript exons

ENST00000352861 — 4 exons

Exon	Start	End
ENSE00001084924	54759158	54759211
ENSE00003644295	54757495	54757594
ENSE00003889608	54755779	54755881
ENSE00003892617	54752253	54752420

Expression profiles

Bgee: expression breadth ubiquitous, 293 present calls, max score 99.32.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 171.4870 / max 1512.7578, expressed in 1825 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
84131	139.7857	1824
84132	26.5168	1808
84130	5.1845	1604

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
pituitary gland	UBERON:0000007	99.32	gold quality
body of pancreas	UBERON:0001150	99.32	gold quality
adenohypophysis	UBERON:0002196	99.27	gold quality
islet of Langerhans	UBERON:0000006	99.16	gold quality
mucosa of transverse colon	UBERON:0004991	98.98	gold quality
corpus epididymis	UBERON:0004359	98.94	gold quality
ganglionic eminence	UBERON:0004023	98.92	gold quality
cartilage tissue	UBERON:0002418	98.89	gold quality
tendon of biceps brachii	UBERON:0008188	98.89	gold quality
ventricular zone	UBERON:0003053	98.88	gold quality
ascending aorta	UBERON:0001496	98.86	gold quality
thoracic aorta	UBERON:0001515	98.86	gold quality
pericardium	UBERON:0002407	98.86	gold quality
left coronary artery	UBERON:0001626	98.84	gold quality
descending thoracic aorta	UBERON:0002345	98.82	gold quality
right coronary artery	UBERON:0001625	98.81	gold quality
aorta	UBERON:0000947	98.77	gold quality
coronary artery	UBERON:0001621	98.74	gold quality
tibial artery	UBERON:0007610	98.72	gold quality
popliteal artery	UBERON:0002250	98.71	gold quality
cortical plate	UBERON:0005343	98.71	gold quality
left lobe of thyroid gland	UBERON:0001120	98.65	gold quality
smooth muscle tissue	UBERON:0001135	98.64	gold quality
calcaneal tendon	UBERON:0003701	98.64	gold quality
tendon	UBERON:0000043	98.63	gold quality
rectum	UBERON:0001052	98.63	gold quality
right testis	UBERON:0004534	98.61	gold quality
pancreas	UBERON:0001264	98.57	gold quality
left uterine tube	UBERON:0001303	98.57	gold quality
embryo	UBERON:0000922	98.54	gold quality

Single-cell (SCXA)

Detected in 21 experiment(s), a significant marker in 17.

Experiment	Marker?	Max mean expression
E-MTAB-6308	yes	13680.65
E-MTAB-8530	yes	2806.45
E-MTAB-10662	yes	1879.22
E-GEOD-84465	yes	498.83
E-HCAD-1	yes	95.17
E-HCAD-4	yes	68.84
E-MTAB-9467	yes	48.69
E-HCAD-5	yes	33.04
E-CURD-46	yes	33.00
E-MTAB-8410	yes	27.73
E-CURD-122	yes	23.32
E-HCAD-9	yes	21.72
E-MTAB-6701	yes	17.80
E-MTAB-10042	yes	13.00
E-MTAB-8142	yes	10.61

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

14 targeting SEC61G, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-651-3P	99.94	73.48	5177
HSA-MIR-488-3P	99.61	68.79	1731
HSA-MIR-4677-3P	99.49	67.91	1246
HSA-MIR-32-3P	99.36	68.20	2517
HSA-MIR-548AS-3P	99.12	69.12	2294
HSA-MIR-3675-3P	99.09	67.70	968
HSA-MIR-7151-3P	99.04	69.72	2370
HSA-MIR-4742-5P	98.89	68.41	1542
HSA-MIR-10395-3P	98.10	66.70	1726
HSA-MIR-6865-3P	97.54	64.67	684
HSA-MIR-630	97.50	66.38	921
HSA-MIR-342-3P	96.44	67.48	1344

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 96.1% of screened cell lines, common-essential.

Literature-anchored findings (GeneRIF, showing 12)

• Cotransin, a substrate-selective Sec61 inhibitor, traps nascent transmembrane domains in the cytosolic vestibule, permitting detailed interrogation of an early pre-integration intermediate. (PMID:24497544)
• Sequencing of childhood ependymoma samples indicate Sec61 gamma subunit protein (SEC61G)-epidermal growth factor receptor (EGFR) chimeric mRNAs in one infratentorial ependymoma , arguing that this fusion occurs in a small proportion of these tumors. (PMID:29092923)
• High expression of SEC61G was significantly correlated with poor prognosis in all GBM patients. High expression of SEC61G was also associated with poor outcome. (PMID:31094363)
• LncRNA LINC02418 regulates proliferation and apoptosis of non-small cell lung cancer cells by regulating miR-4677-3p/SEC61G. (PMID:31841189)
• SEC61G plays an oncogenic role in hepatocellular carcinoma cells. (PMID:33171060)
• SEC61G is upregulated and required for tumor progression in human kidney cancer. (PMID:33846795)
• SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1. (PMID:34039955)
• SEC61G identified as a prognostic biomarker of head and neck squamous cell carcinoma. (PMID:34173014)
• SEC61G overexpression and DNA amplification correlates with prognosis and immune cell infiltration in head and neck squamous cell carcinoma. (PMID:34590792)
• Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation. (PMID:34774014)
• SEC61G Promotes Cervical Cancer Proliferation by Activating MAPK Signaling Pathway. (PMID:36092956)
• Knockdown of circ_0044226 promotes endoplasmic reticulum stress-mediated autophagy and apoptosis in hepatic stellate cells via miR-4677-3p/SEC61G axis. (PMID:38421527)

Cross-species orthologs

12 orthologs

Organism	Symbol	Gene ID
danio_rerio	sec61g	ENSDARG00000018637
mus_musculus	Sec61g	ENSMUSG00000078974
rattus_norvegicus	Sec61g	ENSRNOG00000005203
rattus_norvegicus	AABR07067441.3	ENSRNOG00000052328
rattus_norvegicus	AABR07028668.1	ENSRNOG00000060417
rattus_norvegicus	Sec61g-ps4	ENSRNOG00000061620
rattus_norvegicus		ENSRNOG00000064831
rattus_norvegicus		ENSRNOG00000076767
drosophila_melanogaster	Sec61gamma	FBGN0031049
drosophila_melanogaster	CG8860	FBGN0033691
drosophila_melanogaster	CG13426	FBGN0034510
caenorhabditis_elegans		WBGENE00001303

Protein

Protein identifiers

Protein transport protein Sec61 subunit gamma — P60059 (reviewed: P60059)

All UniProt accessions (1): P60059

UniProt curated annotations — full annotation on UniProt →

Function. Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER.

Subunit / interactions. The SEC61 channel-forming translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63. The SEC61 channel associates with the multi-pass translocon (MPT) complex. (Microbial infection) May interact with Zika virus strain Mr-766 non-structural protein 4A/NS4A. May interact with Zika virus French Polynesia 10087PF/2013 non-structural protein 4A/NS4A. (Microbial infection) May interact with Dengue virus DENV2 16681 non-structural protein 4A/NS4A.

Subcellular location. Endoplasmic reticulum membrane.

Similarity. Belongs to the SecE/SEC61-gamma family.

RefSeq proteins (2): NP_001012474, NP_055117* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001901	Translocase_SecE/Sec61-g	Family
IPR008158	Translocase_Sec61-g	Family
IPR023391	Prot_translocase_SecE_dom_sf	Homologous_superfamily

Pfam: PF00584

UniProt features (8 total): topological domain 2, modified residue 2, helix 2, chain 1, transmembrane region 1

Structure

Experimental structures (PDB)

16 structures.

PDB	Method	Resolution (Å)
8DNZ	ELECTRON MICROSCOPY	2.57
8DNY	ELECTRON MICROSCOPY	2.85
8DO0	ELECTRON MICROSCOPY	2.86
8DO2	ELECTRON MICROSCOPY	2.95
8DNX	ELECTRON MICROSCOPY	2.98
8DO1	ELECTRON MICROSCOPY	3.01
8DNV	ELECTRON MICROSCOPY	3.03
9D6L	ELECTRON MICROSCOPY	3.1
9N9J	ELECTRON MICROSCOPY	3.2
8DO3	ELECTRON MICROSCOPY	3.22
8OJ0	ELECTRON MICROSCOPY	3.3
8OJ8	ELECTRON MICROSCOPY	3.3
8DNW	ELECTRON MICROSCOPY	3.4
6W6L	ELECTRON MICROSCOPY	3.84
9YGY	ELECTRON MICROSCOPY	4.1
8B6L	ELECTRON MICROSCOPY	7.6

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P60059-F1	92.02	0.83

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 1, 18

Function

Pathways and Gene Ontology

Reactome pathways

10 pathways

ID	Pathway
R-HSA-1236974	ER-Phagosome pathway
R-HSA-1799339	SRP-dependent cotranslational protein targeting to membrane
R-HSA-9609523	Insertion of tail-anchored proteins into the endoplasmic reticulum membrane
R-HSA-1236975	Antigen processing-Cross presentation
R-HSA-1280218	Adaptive Immune System
R-HSA-168256	Immune System
R-HSA-392499	Metabolism of proteins
R-HSA-72766	Translation
R-HSA-9609507	Protein localization
R-HSA-983169	Class I MHC mediated antigen processing & presentation

MSigDB gene sets: 202 (showing top): BORCZUK_MALIGNANT_MESOTHELIOMA_UP, REACTOME_ADAPTIVE_IMMUNE_SYSTEM, GOBP_INTRACELLULAR_PROTEIN_TRANSPORT, REACTOME_CLASS_I_MHC_MEDIATED_ANTIGEN_PROCESSING_PRESENTATION, GOBP_PROTEIN_TARGETING, MORF_RAD21, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ENDOPLASMIC_RETICULUM, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ORGANELLE, MORF_PSMC2, PUJANA_CHEK2_PCC_NETWORK, GOBP_PROTEIN_TARGETING_TO_MEMBRANE, MORF_ATOX1, GGAANCGGAANY_UNKNOWN, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_MEMBRANE, YAO_TEMPORAL_RESPONSE_TO_PROGESTERONE_CLUSTER_13

GO Biological Process (5): post-translational protein targeting to membrane, translocation (GO:0031204), protein targeting to ER (GO:0045047), protein targeting (GO:0006605), intracellular protein transport (GO:0006886), protein transport (GO:0015031)

GO Molecular Function (3): transmembrane protein transporter activity (GO:0008320), ribosome binding (GO:0043022), protein binding (GO:0005515)

GO Cellular Component (6): Sec61 translocon complex (GO:0005784), cytosol (GO:0005829), membrane (GO:0016020), Ssh1 translocon complex (GO:0071261), endoplasmic reticulum (GO:0005783), endoplasmic reticulum membrane (GO:0005789)

Reactome top-level categories

Rollup of top-7 pathways:

Category	Pathways
Antigen processing-Cross presentation	1
Translation	1
Protein localization	1
Class I MHC mediated antigen processing & presentation	1
Immune System	1
Metabolism of proteins	1
Adaptive Immune System	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
establishment of protein localization	2
intracellular protein localization	2
translocon complex	2
cytoplasm	2
cellular anatomical structure	2
post-translational protein targeting to endoplasmic reticulum membrane	1
intracellular protein transmembrane transport	1
protein targeting	1
establishment of protein localization to endoplasmic reticulum	1
protein transport	1
intracellular transport	1
transport	1
macromolecule transmembrane transporter activity	1
protein transmembrane transport	1
protein transporter activity	1
ribonucleoprotein complex binding	1
binding	1
endomembrane system	1
intracellular membrane-bounded organelle	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1

Protein interactions and networks

STRING

0 interactions, top by confidence (×1000):

IntAct

52 interactions, top by confidence:

A	B	Type	Score
SEC61G	SFTPC	psi-mi:“MI:0915”(physical association)	0.560
SFTPC	SEC61G	psi-mi:“MI:0915”(physical association)	0.560
SEC61G	CREB3L1	psi-mi:“MI:0915”(physical association)	0.560
SEC61G	LCN2	psi-mi:“MI:0915”(physical association)	0.560
SEC61G	FAM209A	psi-mi:“MI:0915”(physical association)	0.560
SEC61G	TMEM51	psi-mi:“MI:0915”(physical association)	0.560
SEC61G	AQP6	psi-mi:“MI:0915”(physical association)	0.560
PEX12	SEC61G	psi-mi:“MI:0915”(physical association)	0.560
SEC61G	LRRC25	psi-mi:“MI:0915”(physical association)	0.560
SEC61G	CREB3	psi-mi:“MI:0915”(physical association)	0.370
	TMEM223	psi-mi:“MI:0914”(association)	0.350
	HLA-C	psi-mi:“MI:0914”(association)	0.350
CANX	HLA-A	psi-mi:“MI:0914”(association)	0.350
CSNK2B	OSBPL8	psi-mi:“MI:0914”(association)	0.350
DRG1	RPS3A	psi-mi:“MI:0914”(association)	0.350
CCDC47	ESYT2	psi-mi:“MI:0914”(association)	0.350
ILF3	RPS3A	psi-mi:“MI:0914”(association)	0.350
MAPRE1	SCAMP1	psi-mi:“MI:0914”(association)	0.350
PSPC1	MCRIP1	psi-mi:“MI:0914”(association)	0.350
RACK1	RPS3A	psi-mi:“MI:0914”(association)	0.350
RBM39	RPS3A	psi-mi:“MI:0914”(association)	0.350
RBM42	RPS3A	psi-mi:“MI:0914”(association)	0.350
RBM8A	MCRIP1	psi-mi:“MI:0914”(association)	0.350
RPL5	RPS3A	psi-mi:“MI:0914”(association)	0.350
RPS16	MCRIP1	psi-mi:“MI:0914”(association)	0.350
SEC61B	RPS3A	psi-mi:“MI:0914”(association)	0.350
SRP19	RPS3A	psi-mi:“MI:0914”(association)	0.350
SRP68	RPS3A	psi-mi:“MI:0914”(association)	0.350

BioGRID (49): SEC61G (Two-hybrid), SEC61G (Two-hybrid), SEC61G (Reconstituted Complex), SEC61G (Affinity Capture-MS), SEC61G (Synthetic Lethality), SEC61G (Two-hybrid), SEC61G (Two-hybrid), SEC61G (Two-hybrid), LCN2 (Two-hybrid), CREB3L1 (Two-hybrid), TMEM51 (Two-hybrid), LRRC25 (Two-hybrid), SEC61G (Affinity Capture-MS), SEC61G (Affinity Capture-MS), SEC61G (Affinity Capture-RNA)

ESM2 similar proteins: A6H769, A6MMB1, A6MZM2, C9WPN6, F1QGW6, G1SVB0, O60739, P20461, P30742, P41091, P41567, P41568, P48024, P49171, P51971, P53026, P56330, P60058, P60059, P60060, P61220, P61251, P62081, P62082, P62083, P62854, P62855, P62856, P81795, Q0D5W6, Q2KHU8, Q2VIR3, Q3T104, Q4R4X9, Q56JV1, Q5E938, Q5R797, Q5RFF4, Q5RT64, Q5ZMS3

Diamond homologs: O27713, P0DI74, P0DI75, P35179, P38385, P60058, P60059, P60060, Q09827, Q19967, Q3T104, Q54JV6, Q66KU2, Q7SZU9, Q7T207, Q7Z1B8, Q8I7D9, Q8SRW9, Q962X7, Q9C2D4, Q9SMP2, Q9V668, Q9VWE9, Q2NEV8, A5UKV2, Q6LXB7, Q12UQ2, Q46EU5, Q8PY55, Q8TI84

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
SEC61G	“form complex”	“SEC61 complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 39 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
SRP-dependent cotranslational protein targeting to membrane	8	24.3×	2e-07
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)	5	14.8×	2e-03
Translation	6	11.3×	1e-03
Regulation of expression of SLITs and ROBOs	5	10.5×	3e-03

GO biological processes:

GO term	Partners	Fold	FDR
cytoplasmic translation	6	30.9×	1e-05

Disease & clinical

Clinical variants and AI predictions

ClinVar

8 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	4
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

723 predictions. Top by Δscore:

Variant	Effect	Δscore
7:54752416:CACCA:C	acceptor_gain	1.0000
7:54752418:CCA:C	acceptor_gain	1.0000
7:54752419:CAC:C	acceptor_gain	1.0000
7:54752421:C:CC	acceptor_gain	1.0000
7:54755770:TTTAC:T	donor_loss	1.0000
7:54755771:TTACT:T	donor_loss	1.0000
7:54755772:TACTT:T	donor_loss	1.0000
7:54755773:ACTTA:A	donor_loss	1.0000
7:54755774:C:CA	donor_loss	1.0000
7:54755775:T:TG	donor_loss	1.0000
7:54755777:A:AC	donor_gain	1.0000
7:54755777:ACACA:A	donor_loss	1.0000
7:54755778:C:CA	donor_gain	1.0000
7:54755778:CA:C	donor_gain	1.0000
7:54755778:CACA:C	donor_gain	1.0000
7:54755778:CACAA:C	donor_gain	1.0000
7:54755880:TT:T	acceptor_gain	1.0000
7:54755882:C:CC	acceptor_gain	1.0000
7:54755888:T:C	acceptor_gain	1.0000
7:54757490:ATTAC:A	donor_loss	1.0000
7:54757491:TTA:T	donor_loss	1.0000
7:54757492:TA:T	donor_loss	1.0000
7:54757494:C:CT	donor_loss	1.0000
7:54757591:CTGC:C	acceptor_gain	1.0000
7:54757592:TGC:T	acceptor_gain	1.0000
7:54757595:C:CC	acceptor_gain	1.0000
7:54752417:ACCA:A	acceptor_gain	0.9900
7:54752417:ACCAC:A	acceptor_loss	0.9900
7:54752418:CCAC:C	acceptor_gain	0.9900
7:54752418:CCACT:C	acceptor_loss	0.9900

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000155095 (7:54756566 T>C), RS1000943817 (7:54756740 T>G), RS1001203345 (7:54759404 T>C), RS1001376226 (7:54756956 TAC>T), RS1001700578 (7:54754002 G>A), RS1002319753 (7:54758368 A>G), RS1002578709 (7:54756196 T>C,G), RS1002892878 (7:54759038 C>A), RS1002981079 (7:54760487 A>G), RS1003038001 (7:54758851 G>A,C), RS1003234823 (7:54760185 T>C), RS1003277076 (7:54760716 T>C), RS1003376402 (7:54755364 G>A), RS1003427202 (7:54755165 A>G), RS1004196236 (7:54754900 A>T)

Disease associations

OMIM: gene MIM:609215 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST002463_15	Systemic lupus erythematosus	2.000000e-06
GCST002690_9	Very long-chain saturated fatty acid levels (fatty acid 20:0)	8.000000e-06
GCST009391_896	Metabolite levels	3.000000e-06

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0006796	very long-chain saturated fatty acid measurement
EFO:0010441	triacylglycerol 58:7 measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067244 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.50	Kd	3174	nM	CHEMBL5653589
5.50	ED50	3174	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149369: Binding affinity to human SEC61G incubated for 45 mins by Kinobead based pull down assay	kd	3.1742	uM

CTD chemical–gene interactions

40 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, increases expression	2
sodium arsenite	increases expression	2
Air Pollutants	decreases expression, increases abundance	2
Tobacco Smoke Pollution	increases expression	2
Cyclosporine	increases expression	2
Particulate Matter	increases abundance, affects cotreatment, decreases expression	2
aristolochic acid I	decreases expression	1
dicrotophos	decreases expression	1
lead acetate	increases expression	1
beta-lapachone	increases expression	1
arsenite	affects binding, increases reaction	1
butyraldehyde	increases expression	1
zinc chromate	increases abundance, increases expression	1
nickel sulfate	increases expression	1
nivalenol	decreases expression	1
isobutyl alcohol	affects cotreatment, decreases expression, increases abundance	1
chromium hexavalent ion	increases abundance, increases expression	1
chloropicrin	increases expression	1
Ethanol	affects cotreatment, decreases expression, increases abundance	1
Diethylhexyl Phthalate	increases expression	1
Diuron	decreases expression	1
Estradiol	decreases expression	1
Gasoline	affects cotreatment, decreases expression, increases abundance	1
Isoniazid	decreases expression	1
Ivermectin	decreases expression	1
Nicotine	increases expression	1
Phenobarbital	affects expression	1
Phthalic Acids	increases methylation	1
Polycyclic Aromatic Hydrocarbons	affects cotreatment, decreases expression, increases abundance	1
Rotenone	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652411	Binding	Binding affinity to human SEC61G incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3GQ	Abcam HEK293T SEC61G KO	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.