SF3B5

Summary

SF3B5 (splicing factor 3b subunit 5, HGNC:21083) is a protein-coding gene on chromosome 6q24.2, encoding Splicing factor 3B subunit 5 (Q9BWJ5). Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. It is a common-essential gene (DepMap: required in 100.0% of cancer cell lines).

Enables RNA binding activity and splicing factor binding activity. Involved in mRNA splicing, via spliceosome. Located in nucleoplasm. Part of U12-type spliceosomal complex and U2-type precatalytic spliceosome.

Source: NCBI Gene 83443 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 2 total — 1 pathogenic
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 100.0% of screened cell lines (common-essential)
• MANE Select transcript: NM_031287

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:21083
Approved symbol	SF3B5
Name	splicing factor 3b subunit 5
Location	6q24.2
Locus type	gene with protein product
Status	Approved
Aliases	SF3b10, MGC3133, Ysf3
Ensembl gene	ENSG00000169976
Ensembl biotype	protein_coding
OMIM	617847
Entrez	83443

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000367569

RefSeq mRNA: 1 — MANE Select: NM_031287 NM_031287

CCDS: CCDS5204

Canonical transcript exons

ENST00000367569 — 1 exons

Exon	Start	End
ENSE00002049869	144094884	144095573

Expression profiles

Bgee: expression breadth ubiquitous, 286 present calls, max score 98.49.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 147.6282 / max 925.7249, expressed in 1825 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
76054	117.2888	1825
76056	25.7331	1815
76055	1.8071	1299
204230	1.5100	851
76053	1.2892	626

Top tissues by expression

288 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adenohypophysis	UBERON:0002196	98.49	gold quality
mucosa of transverse colon	UBERON:0004991	98.49	gold quality
granulocyte	CL:0000094	98.31	gold quality
right adrenal gland	UBERON:0001233	98.23	gold quality
pituitary gland	UBERON:0000007	98.19	gold quality
left adrenal gland cortex	UBERON:0035825	98.19	gold quality
left adrenal gland	UBERON:0001234	98.16	gold quality
right adrenal gland cortex	UBERON:0035827	98.13	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	98.03	gold quality
adrenal cortex	UBERON:0001235	98.02	gold quality
body of pancreas	UBERON:0001150	97.84	gold quality
skin of leg	UBERON:0001511	97.75	gold quality
adult organism	UBERON:0007023	97.74	gold quality
skin of abdomen	UBERON:0001416	97.72	gold quality
monocyte	CL:0000576	97.66	gold quality
transverse colon	UBERON:0001157	97.65	gold quality
mononuclear cell	CL:0000842	97.64	gold quality
rectum	UBERON:0001052	97.64	gold quality
leukocyte	CL:0000738	97.63	gold quality
apex of heart	UBERON:0002098	97.61	gold quality
gall bladder	UBERON:0002110	97.52	gold quality
left coronary artery	UBERON:0001626	97.49	gold quality
body of stomach	UBERON:0001161	97.47	gold quality
metanephros cortex	UBERON:0010533	97.45	gold quality
islet of Langerhans	UBERON:0000006	97.43	gold quality
lower esophagus	UBERON:0013473	97.42	gold quality
lower esophagus muscularis layer	UBERON:0035833	97.41	gold quality
ganglionic eminence	UBERON:0004023	97.37	gold quality
coronary artery	UBERON:0001621	97.35	gold quality
muscle layer of sigmoid colon	UBERON:0035805	97.35	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-MTAB-6379	no	346.67
E-HCAD-13	no	2.86
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

5 targeting SF3B5, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3688-3P	99.97	72.02	2834
HSA-MIR-4799-5P	99.82	70.60	2663
HSA-MIR-12131	99.48	68.72	1673
HSA-MIR-3182	99.40	68.15	2454
HSA-MIR-4662A-5P	98.48	67.18	1007

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 100.0% of screened cell lines, common-essential.

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	sf3b5	ENSDARG00000016855
mus_musculus	Sf3b5	ENSMUSG00000078348
rattus_norvegicus	Sf3b5	ENSRNOG00000014908
drosophila_melanogaster	Sf3b5	FBGN0040534

Protein

Protein identifiers

Splicing factor 3B subunit 5 — Q9BWJ5 (reviewed: Q9BWJ5)

Alternative names: Pre-mRNA-splicing factor SF3b 10 kDa subunit

All UniProt accessions (1): Q9BWJ5

UniProt curated annotations — full annotation on UniProt →

Function. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for ‘A’ complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.

Subunit / interactions. Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins. Part of the SF3B subcomplex of the 17S U2 SnRNP complex. SF3B associates with the splicing subcomplex SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Within the SF3B subcomplex, interacts directly with SF3B1 (via HEAT domain) and SF3B3. Component of the minor spliceosome, which splices U12-type introns.

Subcellular location. Nucleus.

Similarity. Belongs to the SF3B5 family.

RefSeq proteins (1): NP_112577* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR009846	SF3b5/RDS3-10	Family
IPR017089	Splicing_factor_3B_subunit_5	Family

Pfam: PF07189

UniProt features (13 total): helix 4, modified residue 3, site 2, initiator methionine 1, chain 1, strand 1, region of interest 1

Structure

Experimental structures (PDB)

67 structures, top 30 by resolution.

PDB	Method	Resolution (Å)
7Q4O	ELECTRON MICROSCOPY	2.1
7Q4P	ELECTRON MICROSCOPY	2.15
7Q3L	ELECTRON MICROSCOPY	2.21
7B9C	X-RAY DIFFRACTION	2.4
9RDK	ELECTRON MICROSCOPY	2.41
7EVO	ELECTRON MICROSCOPY	2.5
7EVN	ELECTRON MICROSCOPY	2.6
8H6L	ELECTRON MICROSCOPY	2.6
8H6K	ELECTRON MICROSCOPY	2.7
8HK1	ELECTRON MICROSCOPY	2.7
7DVQ	ELECTRON MICROSCOPY	2.89
7B0I	X-RAY DIFFRACTION	3
7B91	X-RAY DIFFRACTION	3
7B92	X-RAY DIFFRACTION	3
7OMF	X-RAY DIFFRACTION	3
7VPX	ELECTRON MICROSCOPY	3
8I0R	ELECTRON MICROSCOPY	3
8I0T	ELECTRON MICROSCOPY	3
8I0V	ELECTRON MICROSCOPY	3
6EN4	X-RAY DIFFRACTION	3.08
5IFE	X-RAY DIFFRACTION	3.1
7ONB	ELECTRON MICROSCOPY	3.1
7OPI	X-RAY DIFFRACTION	3.1
7QTT	ELECTRON MICROSCOPY	3.1
9K1Y	ELECTRON MICROSCOPY	3.1
8H6E	ELECTRON MICROSCOPY	3.2
8H6J	ELECTRON MICROSCOPY	3.25
9ZE2	ELECTRON MICROSCOPY	3.26
6QX9	ELECTRON MICROSCOPY	3.28
8I0U	ELECTRON MICROSCOPY	3.3

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BWJ5-F1	91.54	0.79

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (2): 5 (interaction with rna); 20 (interaction with rna)

Post-translational modifications (3): 2, 9, 17

Function

Pathways and Gene Ontology

Reactome pathways

8 pathways

ID	Pathway
R-HSA-72163	mRNA Splicing - Major Pathway
R-HSA-72165	mRNA Splicing - Minor Pathway
R-HSA-9770562	mRNA Polyadenylation
R-HSA-9918481	Dengue Virus-Host Interactions
R-HSA-9943411	CHD1 and CHD2 subfamily
R-HSA-72172	mRNA Splicing
R-HSA-72203	Processing of Capped Intron-Containing Pre-mRNA
R-HSA-8953854	Metabolism of RNA

MSigDB gene sets: 142 (showing top): MODULE_151, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GOBP_REGULATION_OF_DNA_REPAIR, WEI_MYCN_TARGETS_WITH_E_BOX, REACTOME_MRNA_3_END_PROCESSING, REACTOME_PROCESSING_OF_CAPPED_INTRON_CONTAINING_PRE_MRNA, GOBP_REGULATION_OF_RESPONSE_TO_STRESS, GOBP_PROTEIN_RNA_COMPLEX_ORGANIZATION, GOBP_RNA_SPLICING, GOBP_DNA_DAMAGE_RESPONSE, YAO_TEMPORAL_RESPONSE_TO_PROGESTERONE_CLUSTER_13, REACTOME_MRNA_SPLICING, BYSTRYKH_HEMATOPOIESIS_STEM_CELL_AND_BRAIN_QTL_TRANS, GOBP_REGULATION_OF_CELLULAR_RESPONSE_TO_STRESS, GRYDER_PAX3FOXO1_ENHANCERS_IN_TADS

GO Biological Process (7): mRNA splicing, via spliceosome (GO:0000398), regulation of DNA repair (GO:0006282), regulation of RNA splicing (GO:0043484), positive regulation of DNA-templated transcription (GO:0045893), U2-type prespliceosome assembly (GO:1903241), mRNA processing (GO:0006397), RNA splicing (GO:0008380)

GO Molecular Function (3): RNA binding (GO:0003723), splicing factor binding (GO:1990935), protein binding (GO:0005515)

GO Cellular Component (9): SAGA complex (GO:0000124), nucleus (GO:0005634), nucleoplasm (GO:0005654), spliceosomal complex (GO:0005681), U2-type spliceosomal complex (GO:0005684), U2 snRNP (GO:0005686), U12-type spliceosomal complex (GO:0005689), U2-type precatalytic spliceosome (GO:0071005), precatalytic spliceosome (GO:0071011)

Reactome top-level categories

Rollup of top-6 pathways:

Category	Pathways
mRNA Splicing	2
mRNA 3’-end processing	1
Dengue Virus Infection	1
CHD chromatin remodelers	1
Processing of Capped Intron-Containing Pre-mRNA	1
Metabolism of RNA	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
spliceosomal complex	3
RNA processing	2
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	1
mRNA processing	1
DNA repair	1
regulation of DNA metabolic process	1
regulation of cellular response to stress	1
RNA splicing	1
regulation of gene expression	1
regulation of primary metabolic process	1
DNA-templated transcription	1
regulation of DNA-templated transcription	1
positive regulation of RNA biosynthetic process	1
spliceosomal complex assembly	1
mRNA metabolic process	1
nucleic acid binding	1
protein binding	1
binding	1
SAGA-type complex	1
DUBm complex	1
peptidase complex	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cellular anatomical structure	1
nuclear protein-containing complex	1
ribonucleoprotein complex	1
spliceosomal snRNP complex	1
U2-type spliceosomal complex	1
U1 snRNP	1
U2 snRNP	1
U4/U6 x U5 tri-snRNP complex	1
precatalytic spliceosome	1

Protein interactions and networks

STRING

2727 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
SF3B5	SF3B3	Q15393	997
SF3B5	SF3B2	Q13435	996
SF3B5	PHF5A	Q7RTV0	995
SF3B5	SF3B4	Q15427	995
SF3B5	SF3B6	Q9Y3B4	992
SF3B5	SF3B1	O75533	973
SF3B5	SF3A2	Q15428	807
SF3B5	SGF29	Q96ES7	806
SF3B5	SF3A3	Q12874	801
SF3B5	SF3A1	Q15459	778
SF3B5	DDX46	Q7L014	653
SF3B5	RBMX2	Q9Y388	617
SF3B5	BUD13	Q9BRD0	580
SF3B5	U2AF2	P26368	532
SF3B5	SNRNP200	O75643	479

IntAct

80 interactions, top by confidence:

A	B	Type	Score
SNRPF	GEMIN2	psi-mi:“MI:0914”(association)	0.910
CSNK1A1	FAM83G	psi-mi:“MI:0914”(association)	0.900
TAF12	TAF4	psi-mi:“MI:0914”(association)	0.760
CCDC97	SF3B1	psi-mi:“MI:0914”(association)	0.730
PHF5A	SF3B1	psi-mi:“MI:0914”(association)	0.730
SNRPD2	GEMIN2	psi-mi:“MI:0914”(association)	0.710
SNRPB	PRMT5	psi-mi:“MI:0914”(association)	0.670
SNRPA1	HTATSF1	psi-mi:“MI:0914”(association)	0.640
DNAJC8	SF3B1	psi-mi:“MI:0914”(association)	0.640
SF3B1	SAP18	psi-mi:“MI:0914”(association)	0.640
SNRPA1	U2SURP	psi-mi:“MI:0914”(association)	0.640
NIPSNAP2	HSPD1	psi-mi:“MI:0914”(association)	0.610
PPP4R2	SF3B1	psi-mi:“MI:0914”(association)	0.570
PPP4R2	SF3B1	psi-mi:“MI:2364”(proximity)	0.570
STAMBPL1	SF3B5	psi-mi:“MI:0915”(physical association)	0.560
TRAPPC6A	SF3B5	psi-mi:“MI:0915”(physical association)	0.560
SF3B5	SF3A2	psi-mi:“MI:0915”(physical association)	0.560
IWS1	SUPT5H	psi-mi:“MI:0914”(association)	0.530
SNRPE	PRMT5	psi-mi:“MI:0914”(association)	0.530
SNRPN	PRMT5	psi-mi:“MI:0914”(association)	0.530
RPS2	MPHOSPH10	psi-mi:“MI:0914”(association)	0.530
Sf3a1	U2SURP	psi-mi:“MI:0915”(physical association)	0.400
	FER1L5	psi-mi:“MI:0915”(physical association)	0.400
CHTOP	SAP18	psi-mi:“MI:0915”(physical association)	0.400
SF1	U2SURP	psi-mi:“MI:0914”(association)	0.350
ZC3H18	SAP18	psi-mi:“MI:0914”(association)	0.350

BioGRID (231): SF3B5 (Affinity Capture-MS), SF3B5 (Affinity Capture-MS), SF3B5 (Affinity Capture-MS), SF3B5 (Affinity Capture-MS), SF3B5 (Co-fractionation), SF3B5 (Co-fractionation), SF3B5 (Co-fractionation), SF3B5 (Co-fractionation), SF3B5 (Co-fractionation), SF3B5 (Co-fractionation), SF3B5 (Co-fractionation), SF3B5 (Affinity Capture-MS), SF3B5 (Affinity Capture-MS), SF3B5 (Affinity Capture-MS), SF3B5 (Proximity Label-MS)

ESM2 similar proteins: A0AJC4, A2RJA2, A8AYT5, A9VLF7, B7GK26, B7H9G7, B7HTQ3, B7ILV5, B7JT58, B9DTS7, B9J290, C1EW67, C3LB28, C3LP78, C3PCE8, D2Y494, O70454, P12454, P12514, P12805, P19029, P19508, P19745, P34313, P35682, P41223, P42939, P61464, P83517, Q02XJ3, Q2NKU3, Q4L7D2, Q567Z7, Q56K13, Q632P7, Q65FU4, Q65WT0, Q6HC88, Q6PGH1, Q71YZ7

Diamond homologs: P58728, Q55BF5, Q56K13, Q923D4, Q9BWJ5, Q9LW64, Q9P7R6, Q9VHI4, P0C074

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
SF3B5	“form complex”	SF3b	binding
SF3B5	“form complex”	“U2 snRNP complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 92 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Metabolism of non-coding RNA	6	52.9×	4e-08
mRNA Splicing - Minor Pathway	9	28.0×	1e-09
SARS-CoV-2 modulates host translation machinery	9	28.0×	1e-09
mRNA Splicing	18	27.4×	1e-19
CHD1 and CHD2 subfamily	18	27.2×	1e-19
mRNA Polyadenylation	21	25.6×	3e-22
Processing of Capped Intron-Containing Pre-mRNA	19	21.7×	6e-19
snRNP Assembly	7	20.6×	2e-06

GO biological processes:

GO term	Partners	Fold	FDR
U2-type prespliceosome assembly	15	110.1×	8e-26
spliceosomal complex assembly	8	56.6×	1e-10
spliceosomal snRNP assembly	7	47.9×	1e-08
RNA splicing, via transesterification reactions	5	36.7×	1e-05
mRNA splicing, via spliceosome	23	24.8×	1e-23
RNA splicing	19	19.7×	2e-17
regulation of DNA repair	5	16.2×	7e-04
regulation of RNA splicing	5	12.9×	2e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

2 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	0
Uncertain significance	1
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
1071172	NC_000006.11:g.(?143772160)(144508648_?)del	Pathogenic

SpliceAI

184 predictions. Top by Δscore:

Variant	Effect	Δscore
6:144095410:C:CA	donor_gain	0.8800
6:144095449:TGG:T	donor_gain	0.8600
6:144095311:G:A	donor_gain	0.8200
6:144095244:T:A	donor_gain	0.7900
6:144095289:T:A	donor_gain	0.7600
6:144095167:A:AC	donor_gain	0.7500
6:144095168:C:CC	donor_gain	0.7500
6:144095409:TCC:T	donor_gain	0.7500
6:144095410:CCC:C	donor_gain	0.7500
6:144095324:ATTCT:A	donor_gain	0.7300
6:144095446:A:AC	donor_gain	0.6800
6:144095447:C:CC	donor_gain	0.6800
6:144095411:C:A	donor_gain	0.6700
6:144095239:A:AC	donor_gain	0.6400
6:144095105:TCTGT:T	donor_gain	0.6300
6:144095284:T:C	donor_gain	0.6300
6:144095413:A:AC	donor_gain	0.6300
6:144095414:C:CC	donor_gain	0.6300
6:144095240:G:C	donor_gain	0.6200
6:144095091:C:A	donor_gain	0.6100
6:144095090:C:CA	donor_gain	0.5900
6:144095149:T:TA	donor_gain	0.5900
6:144095245:C:CA	donor_gain	0.5900
6:144095397:T:C	donor_gain	0.5900
6:144095448:T:C	donor_gain	0.5900
6:144095415:T:C	donor_gain	0.5700
6:144095330:C:CT	acceptor_gain	0.5600
6:144095377:A:AC	donor_gain	0.5600
6:144095195:CG:C	donor_gain	0.5500
6:144095328:TCC:T	acceptor_gain	0.5400

AlphaMissense

575 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
6:144095388:C:G	R37P	0.999
6:144095407:A:G	W31R	0.999
6:144095407:A:T	W31R	0.999
6:144095433:C:T	G22D	0.999
6:144095439:C:T	G20D	0.999
6:144095389:G:T	R37S	0.998
6:144095439:C:A	G20V	0.998
6:144095440:C:G	G20R	0.998
6:144095457:A:G	L14P	0.998
6:144095310:C:G	R63P	0.997
6:144095382:G:C	S39W	0.997
6:144095383:A:G	S39P	0.997
6:144095434:C:G	G22R	0.997
6:144095447:C:A	K17N	0.997
6:144095447:C:G	K17N	0.997
6:144095452:A:G	S16P	0.997
6:144095385:T:A	D38V	0.996
6:144095337:G:T	A54D	0.995
6:144095365:C:G	G45R	0.995
6:144095384:G:C	D38E	0.995
6:144095384:G:T	D38E	0.995
6:144095385:T:G	D38A	0.995
6:144095386:C:G	D38H	0.995
6:144095440:C:A	G20C	0.995
6:144095449:T:C	K17E	0.995
6:144095274:G:T	P75H	0.994
6:144095338:C:G	A54P	0.994
6:144095427:G:T	A24D	0.994
6:144095463:T:A	E12V	0.994
6:144095270:A:C	C76W	0.993

dbSNP variants (sampled 300 via entrez): RS1001572263 (6:144096617 GGA>G), RS1001670384 (6:144096929 C>G), RS1003195279 (6:144095986 C>G,T), RS1003246359 (6:144095378 G>A,C), RS1003249643 (6:144096303 G>A), RS1003852933 (6:144094487 T>C), RS1005568120 (6:144097342 A>G), RS1005664898 (6:144097500 A>G), RS1006279596 (6:144097335 G>C,T), RS1007204518 (6:144097132 C>T), RS1008879692 (6:144094801 T>A), RS1010972556 (6:144096356 C>A,G), RS1011762012 (6:144096016 T>C), RS1011954743 (6:144095768 G>T), RS1012268478 (6:144096588 A>G)

Disease associations

OMIM: gene MIM:617847 | disease phenotypes: MIM:603552

GenCC curated gene-disease

Mondo (1): familial hemophagocytic lymphohistiocytosis 4 (MONDO:0011336)

Orphanet (1): Familial hemophagocytic lymphohistiocytosis (Orphanet:540)

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

MeSH disease descriptors (1)

Descriptor	Name	Tree numbers
C537252	Hemophagocytic lymphohistiocytosis, familial, 4 (supp.)

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067256 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

31 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	affects expression, decreases expression, increases expression	4
Valproic Acid	affects expression, increases expression	3
Cyclosporine	increases expression, increases methylation	3
aristolochic acid I	increases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	increases expression	1
terbufos	decreases methylation	1
arsenite	affects binding, increases reaction	1
methylparaben	decreases expression	1
o,p’-DDT	increases expression	1
cobaltous chloride	decreases expression	1
perfluorooctane sulfonic acid	increases expression	1
CGP 52608	affects binding, increases reaction	1
chloropicrin	increases expression	1
2-palmitoylglycerol	increases expression	1
K 7174	decreases expression	1
ICG 001	decreases expression	1
Resveratrol	increases expression	1
Benzo(a)pyrene	affects methylation	1
Doxorubicin	increases expression	1
Fonofos	decreases methylation	1
Ethyl Methanesulfonate	decreases expression	1
Hydrogen Peroxide	affects expression	1
Ivermectin	decreases expression	1
Parathion	decreases methylation	1
Smoke	decreases expression	1
Vitamin E	increases expression	1
Zearalenone	increases expression	1
Sodium Selenite	increases expression	1
Copper Sulfate	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652430	Binding	Binding affinity to human SF3B5 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.