SFXN1

Summary

SFXN1 (sideroflexin 1, HGNC:16085) is a protein-coding gene on chromosome 5q35.2, encoding Sideroflexin-1 (Q9H9B4). Amino acid transporter importing serine, an essential substrate of the mitochondrial branch of the one-carbon pathway, into mitochondria.

Enables L-alanine transmembrane transporter activity and L-serine transmembrane transporter activity. Involved in L-alanine transport; L-serine transport; and serine import into mitochondrion. Located in mitochondrial inner membrane.

Source: NCBI Gene 94081 — RefSeq curated summary.

At a glance

• GWAS associations: 5
• Clinical variants (ClinVar): 54 total
• Druggable target: yes
• MANE Select transcript: NM_022754

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:16085
Approved symbol	SFXN1
Name	sideroflexin 1
Location	5q35.2
Locus type	gene with protein product
Status	Approved
Aliases	FLJ12876, SLC56A1
Ensembl gene	ENSG00000164466
Ensembl biotype	protein_coding
OMIM	615569
Entrez	94081

Gene structure

Transcript identifiers

Ensembl transcripts: 21 — 13 protein_coding, 5 retained_intron, 2 nonsense_mediated_decay, 1 protein_coding_CDS_not_defined

ENST00000321442, ENST00000421887, ENST00000502393, ENST00000502865, ENST00000506963, ENST00000507017, ENST00000507395, ENST00000507823, ENST00000508290, ENST00000513725, ENST00000515736, ENST00000515791, ENST00000873630, ENST00000873631, ENST00000873632, ENST00000939193, ENST00000939194, ENST00000939195, ENST00000939196, ENST00000948990, ENST00000948991

RefSeq mRNA: 7 — MANE Select: NM_022754 NM_001322977, NM_001322978, NM_001322980, NM_001322981, NM_001322982, NM_001322983, NM_022754

CCDS: CCDS4394

Canonical transcript exons

ENST00000321442 — 11 exons

Exon	Start	End
ENSE00001305601	175526638	175529742
ENSE00001516416	175478560	175478639
ENSE00003485461	175513463	175513590
ENSE00003521077	175492095	175492267
ENSE00003535494	175521919	175521968
ENSE00003568277	175512111	175512196
ENSE00003578952	175516614	175516663
ENSE00003620446	175522375	175522422
ENSE00003639509	175511451	175511526
ENSE00003691244	175509032	175509202
ENSE00003785563	175510109	175510207

Expression profiles

Bgee: expression breadth ubiquitous, 281 present calls, max score 96.85.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 42.2753 / max 282.8391, expressed in 1813 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
60355	39.1212	1806
60354	2.4945	1263
60353	0.4753	247
60357	0.1287	33
60356	0.0556	24

Top tissues by expression

285 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
adrenal tissue	UBERON:0018303	96.85	gold quality
amniotic fluid	UBERON:0000173	96.03	gold quality
buccal mucosa cell	CL:0002336	95.59	gold quality
ventricular zone	UBERON:0003053	94.66	gold quality
liver	UBERON:0002107	94.15	gold quality
stromal cell of endometrium	CL:0002255	93.79	gold quality
right lobe of liver	UBERON:0001114	93.70	gold quality
ganglionic eminence	UBERON:0004023	93.47	gold quality
nephron tubule	UBERON:0001231	93.40	gold quality
endothelial cell	CL:0000115	93.33	gold quality
tendon of biceps brachii	UBERON:0008188	92.93	gold quality
islet of Langerhans	UBERON:0000006	92.83	gold quality
cortical plate	UBERON:0005343	91.72	gold quality
rectum	UBERON:0001052	90.88	gold quality
prefrontal cortex	UBERON:0000451	90.82	gold quality
cerebellar hemisphere	UBERON:0002245	90.14	gold quality
cerebellar cortex	UBERON:0002129	90.13	gold quality
choroid plexus epithelium	UBERON:0003911	90.13	gold quality
tonsil	UBERON:0002372	89.97	gold quality
medial globus pallidus	UBERON:0002477	89.93	gold quality
lymph node	UBERON:0000029	89.82	gold quality
right hemisphere of cerebellum	UBERON:0014890	89.81	gold quality
primary visual cortex	UBERON:0002436	89.68	gold quality
cerebellum	UBERON:0002037	89.67	gold quality
adult mammalian kidney	UBERON:0000082	89.56	gold quality
embryo	UBERON:0000922	89.28	gold quality
kidney	UBERON:0002113	89.26	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	89.06	gold quality
globus pallidus	UBERON:0001875	89.03	gold quality
oviduct epithelium	UBERON:0004804	89.00	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-CURD-88	yes	35.85
E-ANND-3	yes	7.09
E-HCAD-29	no	539.76

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

114 targeting SFXN1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-429	100.00	73.44	2698
HSA-MIR-200B-3P	100.00	73.31	2693
HSA-MIR-200C-3P	100.00	73.35	2685
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-3924	100.00	72.09	2394
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-574-5P	100.00	66.01	989
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-30A-5P	100.00	76.31	3233
HSA-MIR-30B-5P	100.00	76.29	3248
HSA-MIR-30C-5P	100.00	76.29	3248
HSA-MIR-30D-5P	100.00	76.32	3233
HSA-MIR-30E-5P	100.00	76.32	3242
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-373-5P	99.98	75.36	4753
HSA-MIR-616-5P	99.98	75.58	4775
HSA-MIR-32-5P	99.98	75.21	1964
HSA-MIR-92A-3P	99.98	75.21	1960
HSA-MIR-92B-3P	99.98	75.25	1955
HSA-MIR-25-3P	99.98	74.60	1817
HSA-MIR-363-3P	99.98	74.72	1821
HSA-MIR-367-3P	99.98	74.83	1819
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-493-5P	99.96	72.47	2382
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-335-3P	99.93	73.36	4958

Literature-anchored findings (GeneRIF, showing 8)

• SFXN1 functions as a mitochondrial serine transporter in one-carbon metabolism. (PMID:30442778)
• CircSFXN1 regulates the behaviour of trophoblasts and likely mediates preeclampsia. (PMID:32950919)
• The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. (PMID:33730581)
• Overexpression of SFXN1 indicates poor prognosis and promotes tumor progression in lung adenocarcinoma. (PMID:35878532)
• Loss of SFXN1 mitigates lipotoxicity and predicts poor outcome in non-viral hepatocellular carcinoma. (PMID:37296228)
• Comprehensive analysis the prognostic and immune characteristics of mitochondrial transport-related gene SFXN1 in lung adenocarcinoma. (PMID:38233752)
• SFXN1-mediated immune cell infiltration and tumorigenesis in lung adenocarcinoma: A potential therapeutic target. (PMID:38537539)
• Sideroflexin-1 promotes progression and sensitivity to lapatinib in triple-negative breast cancer by inhibiting TOLLIP-mediated autophagic degradation of CIP2A. (PMID:38849012)

Cross-species orthologs

9 orthologs

Organism	Symbol	Gene ID
danio_rerio	sfxn1	ENSDARG00000019963
mus_musculus	Sfxn1	ENSMUSG00000021474
rattus_norvegicus	Sfxn1	ENSRNOG00000018279
drosophila_melanogaster	Sfxn1-3	FBGN0037239
caenorhabditis_elegans		WBGENE00007080
caenorhabditis_elegans		WBGENE00008137
caenorhabditis_elegans		WBGENE00009513
caenorhabditis_elegans		WBGENE00011440
caenorhabditis_elegans		WBGENE00012402

Paralogs (4): SFXN3 (ENSG00000107819), SFXN5 (ENSG00000144040), SFXN2 (ENSG00000156398), SFXN4 (ENSG00000183605)

Protein

Protein identifiers

Sideroflexin-1 — Q9H9B4 (reviewed: Q9H9B4)

All UniProt accessions (6): Q9H9B4, D6RAE9, D6RDG7, D6RFI0, H0Y9J5, S4R2X2

UniProt curated annotations — full annotation on UniProt →

Function. Amino acid transporter importing serine, an essential substrate of the mitochondrial branch of the one-carbon pathway, into mitochondria. Mitochondrial serine is then converted to glycine and formate, which exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors. May also transport other amino acids including alanine and cysteine.

Subcellular location. Mitochondrion inner membrane.

Tissue specificity. Highly expressed in tissues with high one-carbon metabolism activity, such as blood, liver and kidney.

Similarity. Belongs to the sideroflexin family.

RefSeq proteins (7): NP_001309906, NP_001309907, NP_001309909, NP_001309910, NP_001309911, NP_001309912, NP_073591* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR004686	Mtc	Family

Pfam: PF03820

Catalyzed reactions (Rhea), 3 shown:

• L-cysteine(in) = L-cysteine(out) (RHEA:29655)
• L-serine(in) = L-serine(out) (RHEA:35031)
• L-alanine(in) = L-alanine(out) (RHEA:70719)

UniProt features (17 total): topological domain 6, transmembrane region 5, sequence variant 2, initiator methionine 1, chain 1, modified residue 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H9B4-F1	86.74	0.37

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 2

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 234 (showing top): GOBP_MYELOID_CELL_DIFFERENTIATION, TONKS_TARGETS_OF_RUNX1_RUNX1T1_FUSION_MONOCYTE_UP, GOBP_MYELOID_CELL_HOMEOSTASIS, RODRIGUES_THYROID_CARCINOMA_POORLY_DIFFERENTIATED_UP, GOBP_TRANSITION_METAL_ION_TRANSPORT, GOBP_ERYTHROCYTE_HOMEOSTASIS, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_DN, ACEVEDO_NORMAL_TISSUE_ADJACENT_TO_LIVER_TUMOR_DN, GOBP_AMINO_ACID_TRANSMEMBRANE_TRANSPORT, GOBP_IRON_ION_TRANSPORT, GGCNKCCATNK_UNKNOWN, GOBP_MITOCHONDRIAL_TRANSPORT, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_ORGANIC_ACID_TRANSPORT

GO Biological Process (10): one-carbon metabolic process (GO:0006730), iron ion transport (GO:0006826), L-alanine transport (GO:0015808), L-serine transport (GO:0015825), erythrocyte differentiation (GO:0030218), serine import into mitochondrion (GO:0140300), monoatomic ion transport (GO:0006811), amino acid transport (GO:0006865), monoatomic ion transmembrane transport (GO:0034220), transmembrane transport (GO:0055085)

GO Molecular Function (5): monoatomic ion transmembrane transporter activity (GO:0015075), L-alanine transmembrane transporter activity (GO:0015180), L-serine transmembrane transporter activity (GO:0015194), transmembrane transporter activity (GO:0022857), protein binding (GO:0005515)

GO Cellular Component (4): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), membrane (GO:0016020), mitochondrial membrane (GO:0031966)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
transport	3
L-amino acid transport	2
serine transport	2
transmembrane transport	2
L-amino acid transmembrane transporter activity	2
small molecule metabolic process	1
transition metal ion transport	1
alanine transport	1
myeloid cell differentiation	1
erythrocyte homeostasis	1
amino acid transmembrane transport	1
import into the mitochondrion	1
carboxylic acid transmembrane transport	1
monoatomic ion transport	1
cellular process	1
transmembrane transporter activity	1
monoatomic ion transmembrane transport	1
L-alanine transport	1
alanine transmembrane transporter activity	1
L-serine transport	1
transporter activity	1
binding	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
cellular anatomical structure	1
mitochondrion	1
mitochondrial envelope	1
organelle membrane	1

Protein interactions and networks

STRING

1294 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
SFXN1	GJB1	P08034	687
SFXN1	SLC25A38	Q96DW6	600
SFXN1	SLC25A32	Q9H2D1	459
SFXN1	SLC25A39	Q9BZJ4	415
SFXN1	TIMM29	Q9BSF4	404
SFXN1	SLC25A37	Q9NYZ2	403
SFXN1	DYNC1H1	Q14204	400
SFXN1	AKT1	P31749	394
SFXN1	SFXN2	Q96NB2	392
SFXN1	LYRM7	Q5U5X0	388
SFXN1	LY96	Q9Y6Y9	385
SFXN1	SFXN3	Q9BWM7	382
SFXN1	SOCS3	O14543	377
SFXN1	SHMT2	P34897	375
SFXN1	TLR4	O00206	374

IntAct

195 interactions, top by confidence:

A	B	Type	Score
NDUFS3	NDUFS8	psi-mi:“MI:0914”(association)	0.730
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
SFXN1	DDI1	psi-mi:“MI:0915”(physical association)	0.590
SFXN1	REEP4	psi-mi:“MI:0915”(physical association)	0.560
SFXN1	SLC16A7	psi-mi:“MI:0915”(physical association)	0.560
REEP4	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
PGRMC2	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
SLC10A6	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
SFXN1	SCN3B	psi-mi:“MI:0915”(physical association)	0.560
TLCD4	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
STOM	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
SFXN1	TMEM14B	psi-mi:“MI:0915”(physical association)	0.560
SFXN1	TMX2	psi-mi:“MI:0915”(physical association)	0.560
SLC16A7	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
HSD17B13	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
MMD	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
SFXN1	CIDEB	psi-mi:“MI:0915”(physical association)	0.560
SLC10A1	SFXN1	psi-mi:“MI:0915”(physical association)	0.560
AQP6	SFXN1	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (570): SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), DDI1 (Affinity Capture-MS), SFXN1 (Co-fractionation), SFXN1 (Co-fractionation), SFXN1 (Co-fractionation), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS), SFXN1 (Affinity Capture-MS)

ESM2 similar proteins: A1Z8R8, A5A761, B2LU20, E9QBI7, O13375, O13793, O13899, O14364, O74882, P25710, P32897, P36163, P39952, P40086, P53259, P53311, Q02889, Q10361, Q12029, Q4G2J3, Q4G2J4, Q4HZ95, Q58EK4, Q5E9M8, Q5RD16, Q63965, Q6CFW6, Q6FJJ0, Q6FUG4, Q759E9, Q75F43, Q7S8S5, Q84WG3, Q851X7, Q8IQ70, Q8MKN0, Q8VZ96, Q8VZU9, Q91V61, Q99JR1

Diamond homologs: A5A761, A6QP55, B2LU20, O13793, Q09201, Q12029, Q54NQ9, Q5E9M8, Q5EA43, Q5FC79, Q5RD16, Q63965, Q8CFD0, Q8TD22, Q91V61, Q925N0, Q925N2, Q96NB2, Q99JR1, Q9BWM7, Q9H9B4, Q9JHY2, Q9VN13, Q9VVW3, A8E7G5

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
SFXN1	“up-regulates quantity”	L-serine	relocalization

Disease & clinical

Clinical variants and AI predictions

ClinVar

54 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	36
Likely benign	1
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

2027 predictions. Top by Δscore:

Variant	Effect	Δscore
5:175478637:GCG:G	donor_gain	1.0000
5:175492082:T:TA	acceptor_gain	1.0000
5:175492090:T:A	acceptor_gain	1.0000
5:175492090:TGCA:T	acceptor_loss	1.0000
5:175492091:GCAGT:G	acceptor_loss	1.0000
5:175492092:CAGTC:C	acceptor_loss	1.0000
5:175492093:A:AC	acceptor_loss	1.0000
5:175492093:A:AG	acceptor_gain	1.0000
5:175492093:AGTCC:A	acceptor_gain	1.0000
5:175492094:G:GC	acceptor_gain	1.0000
5:175492094:GT:G	acceptor_gain	1.0000
5:175492094:GTC:G	acceptor_gain	1.0000
5:175492094:GTCC:G	acceptor_gain	1.0000
5:175492094:GTCCG:G	acceptor_gain	1.0000
5:175492264:ACAG:A	donor_loss	1.0000
5:175492267:GGTAA:G	donor_loss	1.0000
5:175492269:T:A	donor_loss	1.0000
5:175509182:T:TA	donor_gain	1.0000
5:175509199:ACAG:A	donor_loss	1.0000
5:175509200:CAG:C	donor_loss	1.0000
5:175509202:GGTA:G	donor_loss	1.0000
5:175509203:GT:G	donor_loss	1.0000
5:175509204:T:G	donor_loss	1.0000
5:175510203:GTCAA:G	donor_gain	1.0000
5:175510204:TC:T	donor_gain	1.0000
5:175510208:G:GG	donor_gain	1.0000
5:175510213:GC:G	donor_gain	1.0000
5:175510259:T:TG	donor_gain	1.0000
5:175511449:A:AG	acceptor_gain	1.0000
5:175511450:G:GG	acceptor_gain	1.0000

AlphaMissense

2120 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
5:175509167:C:A	N100K	1.000
5:175509167:C:G	N100K	1.000
5:175510157:T:A	N128K	1.000
5:175510157:T:G	N128K	1.000
5:175510169:T:A	N132K	1.000
5:175510169:T:G	N132K	1.000
5:175512176:T:A	N192K	1.000
5:175512176:T:G	N192K	1.000
5:175513564:G:T	R233M	1.000
5:175509139:G:A	G91E	0.999
5:175509142:G:C	R92T	0.999
5:175509145:T:C	M93T	0.999
5:175509181:G:A	G105D	0.999
5:175510131:T:A	W120R	0.999
5:175510131:T:C	W120R	0.999
5:175510137:T:A	W122R	0.999
5:175510137:T:C	W122R	0.999
5:175510145:C:A	N124K	0.999
5:175510145:C:G	N124K	0.999
5:175510148:G:C	Q125H	0.999
5:175510148:G:T	Q125H	0.999
5:175510152:T:C	F127L	0.999
5:175510154:C:A	F127L	0.999
5:175510154:C:G	F127L	0.999
5:175510158:G:C	A129P	0.999
5:175510159:C:A	A129D	0.999
5:175510165:T:A	V131D	0.999
5:175510167:A:G	N132D	0.999
5:175510178:C:A	N135K	0.999
5:175510178:C:G	N135K	0.999

dbSNP variants (sampled 300 via entrez): RS1000008053 (5:175486528 A>G), RS1000037758 (5:175515440 A>C), RS1000095326 (5:175507736 C>T), RS1000127874 (5:175478886 C>T), RS1000130445 (5:175529992 T>C), RS1000137032 (5:175517520 C>A), RS1000177220 (5:175480523 C>A), RS1000182630 (5:175514997 C>T), RS1000187095 (5:175521106 T>A), RS1000196852 (5:175520767 C>T), RS1000256274 (5:175525366 T>C), RS1000380225 (5:175489722 A>G), RS1000452430 (5:175527323 G>A), RS1000585922 (5:175494855 A>T), RS1000587013 (5:175525665 A>G)

Disease associations

OMIM: gene MIM:615569 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

5 associations (top):

Study	Trait	p-value
GCST003542_45	Night sleep phenotypes	5.000000e-06
GCST004863_109	Mosquito bite size	2.000000e-07
GCST008163_131	Height	8.000000e-06
GCST009391_10	Metabolite levels	3.000000e-06
GCST009391_1217	Metabolite levels	9.000000e-06

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0008378	mosquito bite reaction size measurement
EFO:0007813	cotinine measurement
EFO:0010420	triacylglycerol 54:2 measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067362 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: transporter — SLC56 Sideroflexins

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.92	Kd	12.02	nM	CHEMBL5653589
7.92	ED50	12.02	nM	CHEMBL5653589
5.46	Kd	3466	nM	CHEMBL3752910
5.46	ED50	3466	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149390: Binding affinity to human SFXN1 incubated for 45 mins by Kinobead based pull down assay	kd	0.0120	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149390: Binding affinity to human SFXN1 incubated for 45 mins by Kinobead based pull down assay	kd	3.4662	uM

CTD chemical–gene interactions

46 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases expression, decreases expression, increases abundance	4
Valproic Acid	decreases expression	3
bisphenol A	decreases expression, increases expression, increases methylation	2
arsenite	affects binding, increases reaction, increases methylation	2
Benzo(a)pyrene	decreases expression, increases methylation	2
Cyclosporine	affects methylation, decreases expression	2
aristolochic acid I	decreases expression	1
triphenyl phosphate	affects expression	1
trichostatin A	increases expression	1
cobaltous chloride	decreases expression	1
butyraldehyde	decreases expression	1
CGP 52608	affects binding, increases reaction	1
calfactant	affects cotreatment, decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
ICG 001	decreases expression	1
bisphenol B	increases expression	1
abrine	decreases expression	1
2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine	decreases expression, increases response to substance	1
LDN 193189	affects cotreatment, increases expression	1
NSC 689534	affects binding, decreases expression	1
bisphenol AF	increases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	decreases expression	1
Acetaminophen	decreases expression	1
Arsenic	increases abundance, increases expression	1
Clozapine	affects cotreatment, decreases expression	1
Copper	affects binding, decreases expression	1
Coumestrol	decreases expression	1
Cuprizone	affects cotreatment, decreases expression	1
Curcumin	decreases expression	1
Diethylstilbestrol	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652432	Binding	Binding affinity to human SFXN1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_D4H8	HCT116-SFXN1-KO-c5	Cancer cell line	Male
CVCL_D4H9	HCT116-SFXN1-KO-c8	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.