SLC35A5

Summary

SLC35A5 (solute carrier family 35 member A5, HGNC:20792) is a protein-coding gene on chromosome 3q13.2, encoding UDP-sugar transporter protein SLC35A5 (Q9BS91). Probable UDP-sugar:UMP transmembrane antiporter involved in UDP-alpha-D-glucuronate/UDP-GlcA, UDP-GlcNAc/UDP-N-acetyl-alpha-D-glucosamine and UDP-N-acetyl-alpha-D-galactosamine/UDP-GalNAc transport from the cytosol to the lumen of the Golgi.

This gene encodes a transmembrane protein which belongs to subfamily 35A of the solute carrier superfamily. The encoded protein is a nucleoside-sugar transporter. Alternative splicing results in multiple transcript variants.

Source: NCBI Gene 55032 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 60 total
• MANE Select transcript: NM_017945

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:20792
Approved symbol	SLC35A5
Name	solute carrier family 35 member A5
Location	3q13.2
Locus type	gene with protein product
Status	Approved
Aliases	FLJ20730
Ensembl gene	ENSG00000138459
Ensembl biotype	protein_coding
OMIM	620298
Entrez	55032

Gene structure

Transcript identifiers

Ensembl transcripts: 21 — 18 protein_coding, 2 protein_coding_CDS_not_defined, 1 retained_intron

ENST00000261034, ENST00000460615, ENST00000460713, ENST00000468642, ENST00000484995, ENST00000492406, ENST00000494706, ENST00000890627, ENST00000890628, ENST00000890629, ENST00000890630, ENST00000890631, ENST00000890632, ENST00000890633, ENST00000931385, ENST00000931386, ENST00000931387, ENST00000931388, ENST00000931389, ENST00000959802, ENST00000959803

RefSeq mRNA: 8 — MANE Select: NM_017945 NM_001348905, NM_001348906, NM_001348907, NM_001348908, NM_001348909, NM_001348910, NM_001348911, NM_017945

CCDS: CCDS2967, CCDS87121

Canonical transcript exons

ENST00000492406 — 7 exons

Exon	Start	End
ENSE00001845623	112562045	112562273
ENSE00001955168	112582671	112585579
ENSE00002348060	112563385	112563533
ENSE00003559311	112573889	112573956
ENSE00003589242	112570540	112570670
ENSE00003639745	112580546	112581326
ENSE00003651242	112569171	112569269

Expression profiles

Bgee: expression breadth ubiquitous, 280 present calls, max score 94.80.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 25.6977 / max 592.0902, expressed in 1790 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
37930	11.4016	1725
37929	10.9678	1748
37931	3.0636	1312
37932	0.2648	92

Top tissues by expression

289 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	94.80	gold quality
corpus callosum	UBERON:0002336	94.46	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	94.35	gold quality
spinal cord	UBERON:0002240	94.08	gold quality
adrenal tissue	UBERON:0018303	93.85	gold quality
cortical plate	UBERON:0005343	93.52	gold quality
pons	UBERON:0000988	93.28	gold quality
islet of Langerhans	UBERON:0000006	92.42	gold quality
gall bladder	UBERON:0002110	92.37	gold quality
monocyte	CL:0000576	92.36	gold quality
mononuclear cell	CL:0000842	92.09	gold quality
leukocyte	CL:0000738	91.92	gold quality
rectum	UBERON:0001052	91.92	gold quality
subthalamic nucleus	UBERON:0001906	91.90	gold quality
calcaneal tendon	UBERON:0003701	91.90	gold quality
prefrontal cortex	UBERON:0000451	91.74	gold quality
inferior vagus X ganglion	UBERON:0005363	91.69	gold quality
ganglionic eminence	UBERON:0004023	91.63	gold quality
oocyte	CL:0000023	91.60	gold quality
pigmented layer of retina	UBERON:0001782	90.87	gold quality
retina	UBERON:0000966	90.85	gold quality
superior vestibular nucleus	UBERON:0007227	90.85	gold quality
secondary oocyte	CL:0000655	90.55	gold quality
medulla oblongata	UBERON:0001896	90.39	gold quality
jejunal mucosa	UBERON:0000399	90.14	gold quality
Brodmann (1909) area 9	UBERON:0013540	90.01	gold quality
stromal cell of endometrium	CL:0002255	89.98	gold quality
placenta	UBERON:0001987	89.97	gold quality
ventricular zone	UBERON:0003053	89.92	gold quality
substantia nigra pars compacta	UBERON:0001965	89.72	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-CURD-112	yes	7.29
E-ANND-3	yes	6.31

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

108 targeting SLC35A5, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-30B-5P	100.00	76.29	3248
HSA-MIR-30C-5P	100.00	76.29	3248
HSA-MIR-30A-5P	100.00	76.31	3233
HSA-MIR-30D-5P	100.00	76.32	3233
HSA-MIR-30E-5P	100.00	76.32	3242
HSA-MIR-432-3P	100.00	67.86	705
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-511-3P	99.99	68.85	1467
HSA-MIR-548AW	99.99	72.57	3559
HSA-MIR-485-3P	99.98	70.68	1585
HSA-MIR-539-3P	99.98	70.74	1616
HSA-MIR-4482-3P	99.98	72.50	3147
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-1468-3P	99.96	72.74	3797
HSA-MIR-545-3P	99.95	70.74	2783
HSA-MIR-23A-3P	99.95	74.24	3163
HSA-MIR-23B-3P	99.95	74.24	3163
HSA-MIR-23C	99.95	73.92	3192
HSA-MIR-141-3P	99.94	72.79	2421
HSA-MIR-200A-3P	99.94	72.68	2420
HSA-MIR-144-3P	99.94	73.98	2698
HSA-MIR-381-3P	99.93	71.87	2854
HSA-MIR-515-5P	99.92	69.82	2343
HSA-MIR-519E-5P	99.92	69.62	2358
HSA-MIR-300	99.92	71.76	2856
HSA-MIR-205-3P	99.92	69.92	3165

Literature-anchored findings (GeneRIF, showing 1)

• Study provide evidence suggesting that SLC35A5 might be the first mammalian nucleotide sugar transport (NST) engaged in the transport of three different UDP-sugars. It contains several diacidic motifs within its cytosolically exposed C-terminus, some of which might be capable of UDP-sugar binding. SLC35A5 protein might be a Golgi-resident multi-protein complex member engaged in NST. (PMID:30641943)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	slc35a5	ENSDARG00000000001
mus_musculus	Slc35a5	ENSMUSG00000022664
rattus_norvegicus	Slc35a5	ENSRNOG00000002069
drosophila_melanogaster	senju	FBGN0031676

Paralogs (4): SLC35A2 (ENSG00000102100), SLC35A3 (ENSG00000117620), SLC35A1 (ENSG00000164414), SLC35A4 (ENSG00000176087)

Protein

Protein identifiers

UDP-sugar transporter protein SLC35A5 — Q9BS91 (reviewed: Q9BS91)

Alternative names: Solute carrier family 35 member A5

All UniProt accessions (4): C9J7U8, C9JXZ8, Q9BS91, H7BXF8

UniProt curated annotations — full annotation on UniProt →

Function. Probable UDP-sugar:UMP transmembrane antiporter involved in UDP-alpha-D-glucuronate/UDP-GlcA, UDP-GlcNAc/UDP-N-acetyl-alpha-D-glucosamine and UDP-N-acetyl-alpha-D-galactosamine/UDP-GalNAc transport from the cytosol to the lumen of the Golgi.

Subunit / interactions. Probably forms homooligomers and heterooligomers with SLC35A1, SLC35A2, SLC35A3 and SLC35A4.

Subcellular location. Golgi apparatus membrane.

Similarity. Belongs to the nucleotide-sugar transporter family. SLC35A subfamily.

RefSeq proteins (8): NP_001335834, NP_001335835, NP_001335836, NP_001335837, NP_001335838, NP_001335839, NP_001335840, NP_060415* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007271	Nuc_sug_transpt	Family
IPR037185	EmrE-like	Homologous_superfamily

Pfam: PF04142

Catalyzed reactions (Rhea), 3 shown:

• UMP(out) + UDP-N-acetyl-alpha-D-glucosamine(in) = UMP(in) + UDP-N-acetyl-alpha-D-glucosamine(out) (RHEA:72695)
• UMP(out) + UDP-alpha-D-glucuronate(in) = UMP(in) + UDP-alpha-D-glucuronate(out) (RHEA:72727)
• UDP-N-acetyl-alpha-D-galactosamine(in) + UMP(out) = UDP-N-acetyl-alpha-D-galactosamine(out) + UMP(in) (RHEA:72735)

UniProt features (29 total): topological domain 11, transmembrane region 10, modified residue 3, chain 1, region of interest 1, compositionally biased region 1, glycosylation site 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BS91-F1	76.54	0.38

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 394, 416, 419

Glycosylation sites (1): 204

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 146 (showing top): GSE18804_BRAIN_VS_COLON_TUMORAL_MACROPHAGE_UP, TGCGCANK_UNKNOWN, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_UP, GOBP_ORGANOPHOSPHATE_ESTER_TRANSPORT, GOMF_NUCLEOBASE_CONTAINING_COMPOUND_TRANSMEMBRANE_TRANSPORTER_ACTIVITY, GOBP_NUCLEOBASE_CONTAINING_COMPOUND_TRANSPORT, SCHAEFFER_PROSTATE_DEVELOPMENT_6HR_UP, CUI_TCF21_TARGETS_2_DN, MODULE_95, GOBP_CARBOHYDRATE_DERIVATIVE_TRANSPORT, GOBP_TRANSMEMBRANE_TRANSPORT, BALDWIN_PRKCI_TARGETS_UP, SCGGAAGY_ELK1_02, ARNT_01

GO Biological Process (2): transmembrane transport (GO:0055085), pyrimidine nucleotide-sugar transmembrane transport (GO:0090481)

GO Molecular Function (4): pyrimidine nucleotide-sugar transmembrane transporter activity (GO:0015165), antiporter activity (GO:0015297), transmembrane transporter activity (GO:0022857), protein binding (GO:0005515)

GO Cellular Component (3): Golgi membrane (GO:0000139), Golgi apparatus (GO:0005794), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
transport	1
cellular process	1
nucleotide-sugar transmembrane transport	1
nucleotide-sugar transmembrane transporter activity	1
pyrimidine nucleotide-sugar transmembrane transport	1
secondary active transmembrane transporter activity	1
transporter activity	1
transmembrane transport	1
binding	1
Golgi apparatus	1
bounding membrane of organelle	1
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
cellular anatomical structure	1

Protein interactions and networks

STRING

974 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
SLC35A5	SLC35B4	Q969S0	673
SLC35A5	SLC35C1	Q96A29	605
SLC35A5	SLC35D3	Q5M8T2	586
SLC35A5	SLC35B1	P78383	583
SLC35A5	SLC35B3	Q9H1N7	582
SLC35A5	SLC35D2	Q76EJ3	576
SLC35A5	SLC35H1	Q9NQQ7	570
SLC35A5	SLC35D1	Q9NTN3	560
SLC35A5	SLC35E1	Q96K37	548
SLC35A5	SLC35F1	Q5T1Q4	543
SLC35A5	ITM2C	Q9NQX7	525
SLC35A5	SLC4A5	Q9BY07	508
SLC35A5	SLC33A1	O00400	507
SLC35A5	SLC30A3	Q99726	470
SLC35A5	SYS1	Q8N2H4	451

IntAct

22 interactions, top by confidence:

A	B	Type	Score
SLC35A5	TNKS	psi-mi:“MI:0914”(association)	0.640
TMEM72	SLC35A5	psi-mi:“MI:0915”(physical association)	0.560
SLC35A5	TMEM100	psi-mi:“MI:0915”(physical association)	0.560
FKBP8	SLC35A5	psi-mi:“MI:0915”(physical association)	0.560
SLC35A5	SACM1L	psi-mi:“MI:0915”(physical association)	0.560
SLC35A5	ACTL6B	psi-mi:“MI:0914”(association)	0.530
SLC35A5	HNRNPU	psi-mi:“MI:0915”(physical association)	0.400
SLC35A5		psi-mi:“MI:0915”(physical association)	0.370
LGALS8	SLC22A23	psi-mi:“MI:0914”(association)	0.350
CANX	HLA-A	psi-mi:“MI:0914”(association)	0.350
SLC35A5	CLGN	psi-mi:“MI:0914”(association)	0.350
TMEM100	SLC35A5	psi-mi:“MI:0915”(physical association)	0.000
FKBP8	SLC35A5	psi-mi:“MI:0915”(physical association)	0.000
SACM1L	SLC35A5	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (68): SLC35A5 (Affinity Capture-MS), MBOAT7 (Affinity Capture-MS), TNKS2 (Affinity Capture-MS), TNKS (Affinity Capture-MS), CSGALNACT2 (Affinity Capture-MS), SLC41A1 (Affinity Capture-MS), RHOB (Affinity Capture-MS), TNKS2 (Affinity Capture-MS), TNKS (Affinity Capture-MS), CSGALNACT2 (Affinity Capture-MS), SLC35A5 (Affinity Capture-MS), MBOAT7 (Affinity Capture-MS), ACTL6B (Affinity Capture-MS), SLC35A5 (Two-hybrid), TMEM100 (Two-hybrid)

ESM2 similar proteins: A1L272, A2RV80, A4IF30, A6QL92, A6QPI1, O02777, O80605, P17200, P20272, P21554, P47746, P51810, P56971, P70259, Q1LZI2, Q2V4F9, Q3TDN0, Q3UGM2, Q4R794, Q5F383, Q5FVJ3, Q5IS73, Q5R4D7, Q5R6J3, Q5RD30, Q66H88, Q6P0E8, Q6P499, Q6R5J2, Q71SP5, Q8BGN5, Q8BZK4, Q8CBH5, Q8IY50, Q8NA31, Q8NBV4, Q8R314, Q8RWF4, Q8WV83, Q91WB2

Diamond homologs: A6QPI1, Q5R4D7, Q6DCG9, Q90X48, Q921R7, Q9BS91

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

60 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	50
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1230 predictions. Top by Δscore:

Variant	Effect	Δscore
3:112561451:GCTTA:G	donor_loss	1.0000
3:112561452:CTTA:C	donor_loss	1.0000
3:112561453:TTA:T	donor_loss	1.0000
3:112561454:TACCT:T	donor_loss	1.0000
3:112561455:ACCT:A	donor_loss	1.0000
3:112561456:C:G	donor_loss	1.0000
3:112569265:GAAAG:G	donor_gain	1.0000
3:112570538:A:G	acceptor_gain	1.0000
3:112573883:TTCTA:T	acceptor_loss	1.0000
3:112573884:TCTA:T	acceptor_loss	1.0000
3:112573886:TAGGC:T	acceptor_loss	1.0000
3:112573887:A:AG	acceptor_gain	1.0000
3:112573888:G:GG	acceptor_gain	1.0000
3:112573888:G:GT	acceptor_loss	1.0000
3:112573888:GGCC:G	acceptor_gain	1.0000
3:112573954:GAA:G	donor_gain	1.0000
3:112573955:AAGTA:A	donor_loss	1.0000
3:112573956:AGT:A	donor_loss	1.0000
3:112573957:G:C	donor_loss	1.0000
3:112573957:G:GG	donor_gain	1.0000
3:112573958:TA:T	donor_loss	1.0000
3:112573959:AA:A	donor_loss	1.0000
3:112575216:G:GT	donor_gain	1.0000
3:112562094:G:GT	donor_gain	0.9900
3:112562271:G:GT	donor_gain	0.9900
3:112562271:G:T	donor_gain	0.9900
3:112569164:ATTTC:A	acceptor_loss	0.9900
3:112569165:TTTCA:T	acceptor_loss	0.9900
3:112569166:TTCAG:T	acceptor_loss	0.9900
3:112569167:TCAGA:T	acceptor_loss	0.9900

AlphaMissense

2815 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:112569226:G:C	K62N	0.994
3:112569226:G:T	K62N	0.994
3:112581066:G:C	G317R	0.994
3:112581088:T:C	L324P	0.993
3:112570599:T:A	W97R	0.991
3:112570599:T:C	W97R	0.991
3:112580921:C:A	N268K	0.991
3:112580921:C:G	N268K	0.991
3:112581058:C:A	A314E	0.990
3:112563501:G:C	R33P	0.989
3:112570612:C:A	A101D	0.989
3:112570634:C:A	N108K	0.988
3:112570634:C:G	N108K	0.988
3:112581079:C:A	A321D	0.988
3:112569236:T:C	C66R	0.987
3:112581093:T:C	F326L	0.987
3:112581095:C:A	F326L	0.987
3:112581095:C:G	F326L	0.987
3:112569224:A:G	K62E	0.986
3:112570609:C:A	P100H	0.985
3:112581067:G:A	G317D	0.985
3:112581070:T:C	L318P	0.985
3:112580835:T:C	C240R	0.984
3:112580854:C:A	A246D	0.984
3:112580878:T:C	L254P	0.984
3:112570644:T:C	F112L	0.983
3:112570646:C:A	F112L	0.983
3:112570646:C:G	F112L	0.983
3:112580922:A:C	S269R	0.983
3:112580924:C:A	S269R	0.983

dbSNP variants (sampled 300 via entrez): RS1000096201 (3:112575513 T>G), RS1000101588 (3:112569400 G>A,T), RS1000164128 (3:112580079 G>A), RS1000365234 (3:112570430 C>A,T), RS1000470889 (3:112567576 A>T), RS1000598261 (3:112560717 A>G), RS1000697268 (3:112574080 T>A,C), RS1000796935 (3:112580370 CATTAA>C), RS1000829358 (3:112565115 T>C), RS1001361266 (3:112567013 T>C,G), RS1001484058 (3:112566499 A>G), RS1001487115 (3:112570026 G>C), RS1001762727 (3:112581784 G>A), RS1001847857 (3:112560140 A>G), RS1001877641 (3:112559967 T>C)

Disease associations

OMIM: gene MIM:620298 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: transporter — SLC35 family of nucleotide sugar transporters

CTD chemical–gene interactions

26 total (human), top 26 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cadmium Chloride	decreases expression, increases abundance, increases expression	2
FR900359	affects phosphorylation	1
dicrotophos	decreases expression	1
arsenite	affects binding, increases reaction	1
potassium chromate(VI)	affects cotreatment, decreases expression	1
epigallocatechin gallate	affects cotreatment, decreases expression	1
polyhexamethyleneguanidine	affects expression	1
deguelin	decreases expression	1
K 7174	increases expression	1
fenpyroximate	decreases expression	1
4-chloro-N-((4-(1,1-dimethylethyl)phenyl)methyl)-3-ethyl-1-methyl-1H-pyrazole-5-carboxamide	decreases expression	1
abrine	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Air Pollutants, Occupational	decreases expression	1
Antimycin A	decreases expression	1
Cadmium	increases abundance, increases expression	1
Caffeine	decreases phosphorylation	1
Doxorubicin	decreases expression	1
Nickel	decreases expression	1
Tetrachlorodibenzodioxin	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Urethane	increases expression	1
Zinc	increases expression	1
Aflatoxin B1	decreases methylation	1
Copper Sulfate	decreases expression	1
Particulate Matter	decreases expression, increases abundance	1

Cellosaurus cell lines

5 cell lines: 5 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2FZ	Abcam HeLa SLC35A5 KO	Cancer cell line	Female
CVCL_D4LZ	HCT116-SLC35A5-KO-c1	Cancer cell line	Male
CVCL_D4M0	HCT116-SLC35A5-KO-c9	Cancer cell line	Male
CVCL_TN08	HAP1 SLC35A5 (-) 1	Cancer cell line	Male
CVCL_TN09	HAP1 SLC35A5 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.