SPCS3

Summary

SPCS3 (signal peptidase complex subunit 3, HGNC:26212) is a protein-coding gene on chromosome 4q34.2, encoding Signal peptidase complex subunit 3 (P61009). Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. It is a common-essential gene (DepMap: required in 95.1% of cancer cell lines).

Involved in signal peptide processing and viral protein processing. Located in endoplasmic reticulum membrane. Part of signal peptidase complex.

Source: NCBI Gene 60559 — RefSeq curated summary.

At a glance

• GWAS associations: 9
• Clinical variants (ClinVar): 29 total — 2 pathogenic
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 95.1% of screened cell lines (common-essential)
• MANE Select transcript: NM_021928

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:26212
Approved symbol	SPCS3
Name	signal peptidase complex subunit 3
Location	4q34.2
Locus type	gene with protein product
Status	Approved
Aliases	FLJ22649, SPC22/23, SPC3, YLR066W, PRO3567
Ensembl gene	ENSG00000129128
Ensembl biotype	protein_coding
OMIM	618854
Entrez	60559

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 2 retained_intron, 1 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000503362, ENST00000507001, ENST00000507678, ENST00000513139

RefSeq mRNA: 1 — MANE Select: NM_021928 NM_021928

CCDS: CCDS54823

Canonical transcript exons

ENST00000503362 — 5 exons

Exon	Start	End
ENSE00000886396	176328198	176332245
ENSE00002065140	176319966	176320219
ENSE00003524373	176327162	176327277
ENSE00003530109	176322170	176322243
ENSE00003600295	176324181	176324257

Expression profiles

Bgee: expression breadth ubiquitous, 288 present calls, max score 97.62.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 65.8625 / max 367.3419, expressed in 1823 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
50700	65.3812	1823
50701	0.4813	215

Top tissues by expression

288 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
corpus epididymis	UBERON:0004359	97.62	gold quality
gingival epithelium	UBERON:0001949	97.61	gold quality
pylorus	UBERON:0001166	97.55	gold quality
bone marrow cell	CL:0002092	97.50	gold quality
esophagus squamous epithelium	UBERON:0006920	97.48	gold quality
decidua	UBERON:0002450	97.40	gold quality
body of pancreas	UBERON:0001150	97.37	gold quality
renal glomerulus	UBERON:0000074	97.15	gold quality
gingiva	UBERON:0001828	97.11	gold quality
germinal epithelium of ovary	UBERON:0001304	97.10	gold quality
monocyte	CL:0000576	96.95	gold quality
mononuclear cell	CL:0000842	96.95	gold quality
metanephric glomerulus	UBERON:0004736	96.94	gold quality
substantia nigra pars reticulata	UBERON:0001966	96.87	gold quality
squamous epithelium	UBERON:0006914	96.83	gold quality
leukocyte	CL:0000738	96.81	gold quality
trabecular bone tissue	UBERON:0002483	96.72	gold quality
middle temporal gyrus	UBERON:0002771	96.69	gold quality
cardia of stomach	UBERON:0001162	96.61	gold quality
bone marrow	UBERON:0002371	96.61	gold quality
endometrium	UBERON:0001295	96.60	gold quality
parotid gland	UBERON:0001831	96.50	gold quality
Brodmann (1909) area 23	UBERON:0013554	96.49	gold quality
caput epididymis	UBERON:0004358	96.48	gold quality
upper leg skin	UBERON:0004262	96.46	gold quality
dorsal motor nucleus of vagus nerve	UBERON:0002870	96.40	gold quality
seminal vesicle	UBERON:0000998	96.37	gold quality
trachea	UBERON:0003126	96.33	gold quality
buccal mucosa cell	CL:0002336	96.29	gold quality
tonsil	UBERON:0002372	96.26	gold quality

Single-cell (SCXA)

Detected in 13 experiment(s), a significant marker in 12.

Experiment	Marker?	Max mean expression
E-CURD-88	yes	105.24
E-MTAB-9467	yes	67.08
E-HCAD-1	yes	63.15
E-HCAD-4	yes	60.60
E-CURD-46	yes	54.14
E-CURD-122	yes	49.79
E-MTAB-8410	yes	39.63
E-HCAD-11	yes	23.09
E-HCAD-9	yes	19.59
E-MTAB-10553	yes	10.70
E-HCAD-13	yes	7.01
E-CURD-112	no	3.03
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

199 targeting SPCS3, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-30A-5P	100.00	76.31	3233
HSA-MIR-30B-5P	100.00	76.29	3248
HSA-MIR-30C-5P	100.00	76.29	3248
HSA-MIR-30D-5P	100.00	76.32	3233
HSA-MIR-30E-5P	100.00	76.32	3242
HSA-MIR-126-5P	100.00	72.71	3180
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-4650-5P	99.98	64.69	999
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-1250-3P	99.96	70.04	4038
HSA-MIR-3605-5P	99.96	67.12	932
HSA-MIR-551B-5P	99.96	71.28	3493
HSA-MIR-4725-3P	99.96	69.53	2520
HSA-MIR-6780B-5P	99.96	69.60	2562
HSA-MIR-5688	99.96	73.23	4504
HSA-MIR-495-3P	99.96	72.81	4197
HSA-MIR-559	99.95	72.28	3609

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 95.1% of screened cell lines, common-essential.

Cross-species orthologs

8 orthologs

Organism	Symbol	Gene ID
danio_rerio	spcs3	ENSDARG00000089976
mus_musculus	Arxes2	ENSMUSG00000048040
mus_musculus	Arxes1	ENSMUSG00000048355
mus_musculus	Spcs3	ENSMUSG00000054408
rattus_norvegicus	Spcs3	ENSRNOG00000038933
rattus_norvegicus		ENSRNOG00000078288
drosophila_melanogaster	Spase22-23	FBGN0039172
caenorhabditis_elegans		WBGENE00019679

Protein

Protein identifiers

Signal peptidase complex subunit 3 — P61009 (reviewed: P61009)

Alternative names: Microsomal signal peptidase 22/23 kDa subunit

All UniProt accessions (1): P61009

UniProt curated annotations — full annotation on UniProt →

Function. Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface. (Microbial infection) Plays an important role in virion production of flaviviruses such as West Nile virus, Japanese enchephalitis virus, Dengue virus type 2 and Yellow Fever virus.

Subunit / interactions. Component of the signal peptidase complex paralog A (SPC-A) composed of a catalytic subunit SEC11A and three accessory subunits SPCS1, SPCS2 and SPCS3. Component of the signal peptidase complex paralog C (SPC-C) composed of a catalytic subunit SEC11C and three accessory subunits SPCS1, SPCS2 and SPCS3. Within the complex, interacts with SEC11A or SEC11C and SPCS1. The complex induces a local thinning of the ER membrane which is used to measure the length of the signal peptide (SP) h-region of protein substrates. This ensures the selectivity of the complex towards h-regions shorter than 18-20 amino acids.

Subcellular location. Endoplasmic reticulum membrane.

Similarity. Belongs to the SPCS3 family.

RefSeq proteins (1): NP_068747* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007653	SPC3	Family

Pfam: PF04573

UniProt features (6 total): topological domain 2, chain 1, transmembrane region 1, glycosylation site 1, sequence conflict 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
7P2P	ELECTRON MICROSCOPY	4.9
7P2Q	ELECTRON MICROSCOPY	4.9

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P61009-F1	91.72	0.83

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Glycosylation sites (1): 141

Function

Pathways and Gene Ontology

Reactome pathways

16 pathways

ID	Pathway
R-HSA-1799339	SRP-dependent cotranslational protein targeting to membrane
R-HSA-381771	Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1)
R-HSA-400511	Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP)
R-HSA-422085	Synthesis, secretion, and deacylation of Ghrelin
R-HSA-9768727	Regulation of CDH1 posttranslational processing and trafficking to plasma membrane
R-HSA-9828806	Maturation of hRSV A proteins
R-HSA-9918432	Maturation of DENV proteins
R-HSA-1643685	Disease
R-HSA-2980736	Peptide hormone metabolism
R-HSA-392499	Metabolism of proteins
R-HSA-400508	Incretin synthesis, secretion, and inactivation
R-HSA-5663205	Infectious disease
R-HSA-72766	Translation
R-HSA-9820952	Respiratory Syncytial Virus Infection Pathway
R-HSA-9820965	Respiratory syncytial virus (RSV) genome replication, transcription and translation
R-HSA-9824446	Viral Infection Pathways

MSigDB gene sets: 184 (showing top): RODRIGUES_THYROID_CARCINOMA_POORLY_DIFFERENTIATED_UP, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GOBP_PROTEIN_TARGETING, chr4q34, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ENDOPLASMIC_RETICULUM, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ORGANELLE, REACTOME_ADHERENS_JUNCTIONS_INTERACTIONS, WEI_MYCN_TARGETS_WITH_E_BOX, GOBP_PROTEIN_MATURATION, MORI_PLASMA_CELL_UP, WANG_RESPONSE_TO_FORSKOLIN_UP, IVANOVA_HEMATOPOIESIS_INTERMEDIATE_PROGENITOR, WANG_RESPONSE_TO_ANDROGEN_UP, WANG_PROSTATE_CANCER_ANDROGEN_INDEPENDENT, GOBP_SIGNAL_PEPTIDE_PROCESSING

GO Biological Process (4): obsolete signal peptide processing (GO:0006465), proteolysis (GO:0006508), viral protein processing (GO:0019082), protein targeting to ER (GO:0045047)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (4): endoplasmic reticulum (GO:0005783), signal peptidase complex (GO:0005787), endoplasmic reticulum membrane (GO:0005789), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-11 pathways:

Category	Pathways
Incretin synthesis, secretion, and inactivation	2
Peptide hormone metabolism	2
Metabolism of proteins	2
Translation	1
Regulation of CDH1 Expression and Function	1
Respiratory syncytial virus (RSV) genome replication, transcription and translation	1
Dengue Virus Genome Translation and Replication	1
Disease	1
Viral Infection Pathways	1
Respiratory Syncytial Virus Infection Pathway	1
Infectious disease	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein metabolic process	1
viral process	1
viral gene expression	1
protein targeting	1
establishment of protein localization to endoplasmic reticulum	1
binding	1
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
endoplasmic reticulum membrane	1
membrane protein complex	1
endoplasmic reticulum protein-containing complex	1
serine-type endopeptidase complex	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
cellular anatomical structure	1

Protein interactions and networks

STRING

0 interactions, top by confidence (×1000):

IntAct

84 interactions, top by confidence:

A	B	Type	Score
SEC11C	SPCS3	psi-mi:“MI:0914”(association)	0.820
SEC11C	SPCS3	psi-mi:“MI:0915”(physical association)	0.820
SEC11A	SPCS3	psi-mi:“MI:0914”(association)	0.770
SEC11A	SPCS3	psi-mi:“MI:0915”(physical association)	0.770
GPC1	HADHB	psi-mi:“MI:0914”(association)	0.740
RAB6B	GDI1	psi-mi:“MI:0914”(association)	0.730
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
ATXN1	SPCS3	psi-mi:“MI:0915”(physical association)	0.670
STK4	STRN	psi-mi:“MI:0914”(association)	0.610
HTT	SPCS3	psi-mi:“MI:0915”(physical association)	0.560
SEC11C	APOM	psi-mi:“MI:0914”(association)	0.530
SPCS3	ENTPD6	psi-mi:“MI:0914”(association)	0.530
SEC11A	NPC1	psi-mi:“MI:0914”(association)	0.530
env	PGRMC1	psi-mi:“MI:0914”(association)	0.460

BioGRID (166): SPCS3 (Proximity Label-MS), SPCS3 (Proximity Label-MS), SPCS3 (Proximity Label-MS), SPCS3 (Proximity Label-MS), SPCS3 (Proximity Label-MS), SPCS3 (Proximity Label-MS), SPCS3 (Proximity Label-MS), SPCS3 (Affinity Capture-MS), SPCS3 (Affinity Capture-MS), SPCS3 (Affinity Capture-MS), SPCS3 (Affinity Capture-MS), SPCS3 (Affinity Capture-RNA), SPCS3 (Affinity Capture-MS), SPCS3 (Affinity Capture-MS), SPCS3 (Affinity Capture-MS)

ESM2 similar proteins: A8WG88, C0HK79, C0HK80, E2RQ08, F1NNL1, F1PCT7, F1QR43, F4HTM3, F7B645, O18823, O96005, P04843, P04844, P07153, P0C152, P25235, P28687, P34525, P61008, P61009, P97259, Q08834, Q09328, Q3SZI6, Q3SZU5, Q4R4T0, Q4WGM4, Q53YF3, Q568Z4, Q5AYC8, Q5R9S8, Q5RBM1, Q5RFB6, Q5XIP9, Q60MW2, Q62186, Q6ZWQ7, Q8BR76, Q8BXQ2, Q8R4G6

Diamond homologs: B0G180, C0HK79, C0HK80, P28687, P34525, P61008, P61009, Q12133, Q3SZU5, Q53YF3, Q568Z4, Q60MW2, Q6BPD6, Q6C4R5, Q6ZWQ7, Q9LGB4, Q9MA96, Q9VCA9, Q10259, Q6CRY8

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
SPCS3	“form complex”	“Signal peptidase complex, SEC11C variant”	binding
SPCS3	“form complex”	“Signal peptidase complex, SEC11A variant”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 83 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Regulation of CDH1 posttranslational processing and trafficking to plasma membrane	5	26.7×	3e-04
Maturation of DENV proteins	5	16.8×	2e-03

GO biological processes:

GO term	Partners	Fold	FDR
cilium assembly	7	7.2×	7e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

29 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	2
Likely pathogenic	0
Uncertain significance	14
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (2)

Variant ID	HGVS	Classification
3062803	GRCh37/hg19 4q32.1-35.2(chr4:161589441-190957473)x1	Pathogenic
983279	GRCh37/hg19 4q32.3-35.1(chr4:169108358-184425536)x1	Pathogenic

SpliceAI

682 predictions. Top by Δscore:

Variant	Effect	Δscore
4:176320182:A:T	donor_gain	1.0000
4:176320217:GCT:G	donor_gain	1.0000
4:176320220:G:GG	donor_gain	1.0000
4:176327232:A:G	donor_gain	1.0000
4:176327272:TCTC:T	donor_gain	1.0000
4:176327277:AGT:A	donor_loss	1.0000
4:176327278:G:GC	donor_loss	1.0000
4:176327278:G:GG	donor_gain	1.0000
4:176327279:T:G	donor_loss	1.0000
4:176328302:A:G	donor_gain	1.0000
4:176328324:T:G	donor_gain	1.0000
4:176320216:TGCT:T	donor_gain	0.9900
4:176320217:GCTG:G	donor_gain	0.9900
4:176320218:CT:C	donor_gain	0.9900
4:176320220:GT:G	donor_loss	0.9900
4:176320223:A:AC	donor_loss	0.9900
4:176320224:G:GG	donor_gain	0.9900
4:176325840:TTC:T	donor_gain	0.9900
4:176327157:TATA:T	acceptor_loss	0.9900
4:176327158:ATAGG:A	acceptor_loss	0.9900
4:176327159:TA:T	acceptor_loss	0.9900
4:176327160:A:AG	acceptor_gain	0.9900
4:176327160:AG:A	acceptor_gain	0.9900
4:176327160:AGGC:A	acceptor_loss	0.9900
4:176327161:G:GG	acceptor_gain	0.9900
4:176327161:G:GT	acceptor_loss	0.9900
4:176327161:GG:G	acceptor_gain	0.9900
4:176327161:GGCT:G	acceptor_gain	0.9900
4:176327273:CTCA:C	donor_gain	0.9900
4:176327280:GAG:G	donor_loss	0.9900

AlphaMissense

1171 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
4:176320125:A:C	S17R	0.999
4:176320127:C:A	S17R	0.999
4:176320127:C:G	S17R	0.999
4:176324219:T:C	F86L	0.999
4:176324221:T:A	F86L	0.999
4:176324221:T:G	F86L	0.999
4:176324201:T:A	W80R	0.998
4:176324201:T:C	W80R	0.998
4:176324206:T:A	N81K	0.998
4:176324206:T:G	N81K	0.998
4:176327183:T:A	W106R	0.998
4:176327183:T:C	W106R	0.998
4:176327187:A:C	D107A	0.998
4:176327187:A:T	D107V	0.998
4:176328229:T:A	W148R	0.998
4:176328229:T:C	W148R	0.998
4:176328251:G:A	G155E	0.998
4:176320114:C:A	A13D	0.997
4:176320134:G:C	A20P	0.997
4:176320141:T:C	L22P	0.997
4:176322216:G:A	G64R	0.997
4:176322216:G:C	G64R	0.997
4:176322217:G:A	G64E	0.997
4:176324234:G:C	A91P	0.997
4:176327178:T:A	V104D	0.997
4:176327186:G:C	D107H	0.997
4:176327246:T:C	Y127H	0.997
4:176327274:T:A	L136H	0.997
4:176327274:T:C	L136P	0.997
4:176328248:C:A	A154D	0.997

dbSNP variants (sampled 300 via entrez): RS1000057830 (4:176331920 G>C), RS1000109788 (4:176331742 C>T), RS1000471587 (4:176319879 G>A,C,T), RS1000677858 (4:176318448 G>A), RS1000953710 (4:176324748 G>A), RS1001037646 (4:176330594 G>A), RS1001063750 (4:176330583 G>T), RS1001073879 (4:176330340 T>A,C), RS10013572 (4:176332270 A>C), RS1001386305 (4:176324461 GTTTGTTCTA>G), RS1001612335 (4:176331688 A>C,G), RS1001668696 (4:176319177 T>G), RS1001835496 (4:176318030 T>C), RS1001960493 (4:176325019 T>G), RS1002112796 (4:176325106 C>G,T)

Disease associations

OMIM: gene MIM:618854 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

9 associations (top):

Study	Trait	p-value
GCST003989_18	Chin dimples	3.000000e-15
GCST004659_1	Left ventricular mass	1.000000e-07
GCST005988_4	Serum albumin levels	6.000000e-09
GCST005989_11	Serum total protein levels	4.000000e-09
GCST007354_1	Intracranial aneurysm	6.000000e-13
GCST010796_2093	Electrocardiogram morphology (amplitude at temporal datapoints)	7.000000e-09
GCST010796_2094	Electrocardiogram morphology (amplitude at temporal datapoints)	2.000000e-08
GCST010796_2095	Electrocardiogram morphology (amplitude at temporal datapoints)	4.000000e-08
GCST010796_2096	Electrocardiogram morphology (amplitude at temporal datapoints)	5.000000e-09

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0008205	left ventricular structural measurement
EFO:0004327	electrocardiography

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6066491 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

45 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cyclosporine	increases expression	4
Valproic Acid	affects expression, increases expression	3
Phenylmercuric Acetate	affects cotreatment, increases expression	2
Tobacco Smoke Pollution	increases expression	2
Tunicamycin	increases expression	2
aristolochic acid I	decreases expression	1
methylmercuric chloride	increases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	increases expression	1
pyrogallol 1,3-dimethyl ether	decreases expression, affects cotreatment	1
beta-lapachone	increases expression	1
sodium arsenite	increases expression	1
cobaltous chloride	decreases expression	1
manganese chloride	increases abundance, increases expression	1
potassium chromate(VI)	affects cotreatment, decreases expression	1
cupric chloride	increases expression	1
epigallocatechin gallate	affects cotreatment, decreases expression	1
perfluorooctane sulfonic acid	decreases expression	1
CGP 52608	affects binding, increases reaction	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
ICG 001	decreases expression	1
abrine	decreases expression	1
dorsomorphin	affects cotreatment, increases expression	1
eprenetapopt	affects expression, affects reaction	1
bisphenol S	increases expression	1
bisphenol AF	increases expression	1
Benzo(a)pyrene	increases expression	1
Cisplatin	decreases expression	1
Colforsin	increases expression	1
Furaldehyde	affects cotreatment, increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652513	Binding	Binding affinity to human SPCS3 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2H5	Abcam HeLa SPCS3 KO	Cancer cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.