SPIN1
gene geneOn this page
Also known as TDRD24
Summary
SPIN1 (spindlin 1, HGNC:11243) is a protein-coding gene on chromosome 9q22.1, encoding Spindlin-1 (Q9Y657). Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at ‘Lys-4’ and ‘Lys-9’ (H3K4me3K9me3) and is involved in piRNA-mediated retrotransposon silencing during spermatogenesis.
Enables histone reader activity and methylated histone binding activity. Involved in positive regulation of DNA-templated transcription; positive regulation of Wnt signaling pathway; and rRNA transcription. Located in cytosol and nucleus.
Source: NCBI Gene 10927 — RefSeq curated summary.
At a glance
- GWAS associations: 4
- Clinical variants (ClinVar): 22 total
- Druggable target: yes
- MANE Select transcript:
NM_006717
Identifiers
Gene identifiers
| Field | Value |
|---|---|
| HGNC ID | HGNC:11243 |
| Approved symbol | SPIN1 |
| Name | spindlin 1 |
| Location | 9q22.1 |
| Locus type | gene with protein product |
| Status | Approved |
| Aliases | TDRD24 |
| Ensembl gene | ENSG00000106723 |
| Ensembl biotype | protein_coding |
| OMIM | 609936 |
| Entrez | 10927 |
Gene structure
Transcript identifiers
Ensembl transcripts: 78 — 73 protein_coding, 5 protein_coding_CDS_not_defined
ENST00000375859, ENST00000462844, ENST00000469017, ENST00000483785, ENST00000485565, ENST00000485804, ENST00000853460, ENST00000853461, ENST00000853462, ENST00000853463, ENST00000853464, ENST00000853465, ENST00000853466, ENST00000853467, ENST00000853468, ENST00000853469, ENST00000853470, ENST00000853471, ENST00000853472, ENST00000853473, ENST00000853474, ENST00000853475, ENST00000853476, ENST00000853477, ENST00000853478, ENST00000853479, ENST00000853480, ENST00000853481, ENST00000853482, ENST00000853483, ENST00000853484, ENST00000853485, ENST00000853486, ENST00000853487, ENST00000853488, ENST00000853489, ENST00000853490, ENST00000853491, ENST00000853492, ENST00000853493, ENST00000853494, ENST00000853495, ENST00000853496, ENST00000853497, ENST00000853498, ENST00000853499, ENST00000853500, ENST00000853501, ENST00000853502, ENST00000853503, ENST00000928841, ENST00000928842, ENST00000928843, ENST00000928844, ENST00000928845, ENST00000928846, ENST00000928847, ENST00000928848, ENST00000928849, ENST00000928850, ENST00000928851, ENST00000928852, ENST00000928853, ENST00000960823, ENST00000960824, ENST00000960825, ENST00000960826, ENST00000960827, ENST00000960828, ENST00000960829, ENST00000960830, ENST00000960831, ENST00000960832, ENST00000960833, ENST00000960834, ENST00000960835, ENST00000960836, ENST00000960837
RefSeq mRNA: 1 — MANE Select: NM_006717
NM_006717
CCDS: CCDS43843
Canonical transcript exons
ENST00000375859 — 6 exons
| Exon | Start | End |
|---|---|---|
| ENSE00001468598 | 88475078 | 88478694 |
| ENSE00001876400 | 88388444 | 88388538 |
| ENSE00003477397 | 88468372 | 88468605 |
| ENSE00003592562 | 88462496 | 88462749 |
| ENSE00003642876 | 88426382 | 88426591 |
| ENSE00003679219 | 88448941 | 88448989 |
Expression profiles
Bgee: expression breadth ubiquitous, 279 present calls, max score 99.14.
FANTOM5 (CAGE): breadth ubiquitous, TPM avg 25.4676 / max 330.1331, expressed in 1806 samples.
FANTOM5 promoters (8 alternative TSS)
| Promoter ID | TPM avg | Samples expressed |
|---|---|---|
| 97256 | 18.3974 | 1783 |
| 97255 | 2.8488 | 1156 |
| 97257 | 1.3093 | 739 |
| 97258 | 1.0606 | 614 |
| 97254 | 0.7660 | 369 |
| 97253 | 0.7267 | 414 |
| 97259 | 0.2128 | 80 |
| 97266 | 0.1460 | 28 |
Top tissues by expression
288 total, by Bgee expression score (0-100, higher = more expressed):
| Tissue | Anatomy ID | Expression score | Quality |
|---|---|---|---|
| postcentral gyrus | UBERON:0002581 | 99.14 | gold quality |
| caput epididymis | UBERON:0004358 | 99.06 | gold quality |
| parietal lobe | UBERON:0001872 | 98.93 | gold quality |
| corpus epididymis | UBERON:0004359 | 98.93 | gold quality |
| cortical plate | UBERON:0005343 | 98.62 | gold quality |
| superior vestibular nucleus | UBERON:0007227 | 98.58 | gold quality |
| middle temporal gyrus | UBERON:0002771 | 98.56 | gold quality |
| entorhinal cortex | UBERON:0002728 | 98.42 | gold quality |
| Brodmann (1909) area 23 | UBERON:0013554 | 98.31 | gold quality |
| seminal vesicle | UBERON:0000998 | 98.28 | gold quality |
| renal medulla | UBERON:0000362 | 98.22 | gold quality |
| superior frontal gyrus | UBERON:0002661 | 98.20 | gold quality |
| pons | UBERON:0000988 | 98.19 | gold quality |
| lateral nuclear group of thalamus | UBERON:0002736 | 98.15 | gold quality |
| trigeminal ganglion | UBERON:0001675 | 98.14 | gold quality |
| subthalamic nucleus | UBERON:0001906 | 98.10 | gold quality |
| urethra | UBERON:0000057 | 98.08 | gold quality |
| lateral globus pallidus | UBERON:0002476 | 98.07 | gold quality |
| cauda epididymis | UBERON:0004360 | 98.06 | gold quality |
| ganglionic eminence | UBERON:0004023 | 98.04 | gold quality |
| oocyte | CL:0000023 | 98.02 | gold quality |
| secondary oocyte | CL:0000655 | 98.01 | gold quality |
| globus pallidus | UBERON:0001875 | 97.93 | gold quality |
| mammary duct | UBERON:0001765 | 97.92 | gold quality |
| medial globus pallidus | UBERON:0002477 | 97.91 | gold quality |
| endometrium | UBERON:0001295 | 97.87 | gold quality |
| parietal pleura | UBERON:0002400 | 97.84 | gold quality |
| visceral pleura | UBERON:0002401 | 97.74 | gold quality |
| dorsal root ganglion | UBERON:0000044 | 97.55 | gold quality |
| cerebellar vermis | UBERON:0004720 | 97.48 | gold quality |
Single-cell (SCXA)
Detected in 1 experiment(s), a significant marker in 1.
| Experiment | Marker? | Max mean expression |
|---|---|---|
| E-ANND-3 | yes | 9.24 |
Regulation
Is transcription factor: no
miRNA regulators (miRDB)
240 targeting SPIN1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):
| miRNA | Max score | Avg score | miRNA target_count |
|---|---|---|---|
| HSA-MIR-3163 | 100.00 | 77.23 | 8605 |
| HSA-MIR-6867-5P | 100.00 | 82.21 | 3464 |
| HSA-MIR-3613-3P | 100.00 | 76.36 | 7965 |
| HSA-MIR-8485 | 100.00 | 77.57 | 4731 |
| HSA-MIR-3646 | 100.00 | 73.56 | 5283 |
| HSA-MIR-656-3P | 100.00 | 72.15 | 2788 |
| HSA-MIR-340-5P | 100.00 | 72.50 | 4437 |
| HSA-MIR-190A-3P | 100.00 | 80.35 | 5520 |
| HSA-MIR-5692A | 100.00 | 74.40 | 6850 |
| HSA-LET-7A-3P | 100.00 | 74.03 | 3932 |
| HSA-LET-7B-3P | 100.00 | 74.08 | 3913 |
| HSA-LET-7F-1-3P | 100.00 | 74.02 | 3928 |
| HSA-MIR-98-3P | 100.00 | 74.08 | 3907 |
| HSA-MIR-548AW | 99.99 | 72.57 | 3559 |
| HSA-MIR-548C-3P | 99.99 | 74.01 | 7587 |
| HSA-MIR-4789-3P | 99.99 | 70.75 | 2484 |
| HSA-MIR-6759-5P | 99.99 | 66.54 | 785 |
| HSA-MIR-371B-5P | 99.99 | 75.34 | 4759 |
| HSA-MIR-3662 | 99.99 | 73.82 | 5684 |
| HSA-MIR-33A-5P | 99.99 | 68.62 | 1055 |
| HSA-MIR-33B-5P | 99.99 | 68.58 | 1062 |
| HSA-MIR-513B-5P | 99.99 | 69.96 | 2150 |
| HSA-MIR-4282 | 99.99 | 75.36 | 6408 |
| HSA-MIR-10401-5P | 99.99 | 65.79 | 948 |
| HSA-MIR-3692-3P | 99.98 | 70.27 | 2139 |
| HSA-MIR-4789-5P | 99.98 | 70.76 | 2721 |
| HSA-MIR-373-5P | 99.98 | 75.36 | 4753 |
| HSA-MIR-616-5P | 99.98 | 75.58 | 4775 |
| HSA-MIR-568 | 99.98 | 69.86 | 2084 |
| HSA-MIR-23B-5P | 99.98 | 66.07 | 587 |
Literature-anchored findings (GeneRIF, showing 30)
- spindlin1 gene may contribute to tumorigenesis (PMID:16098913)
- recombinant human spindlin1 has been overexpressed in Escherichia coli BL21, purified and crystallized using the hanging-drop vapour-diffusion method (PMID:16472086)
- analysis of human spindlin1 tudor-like domains for cell cycle regulation (PMID:17082182)
- SPINDLIN1 is an important gene for mammalian mitotic chromosome functions, and disrupted regulation results in abnormal cell division, a mechanism that may be involved in tumorigenesis (PMID:18201843)
- Excess spindlin1 protein may be detrimental for spindle microtubule organization, chromosomal stability and can potentially contribute to the development of cancer. (PMID:18543248)
- Spindlin1 localizes to the active ribosomal DNA (rDNA) repeats, and Spindlin1 facilitates the expression of ribosomal RNA genes. (PMID:21960006)
- SPINDLIN1, which may be a novel substrate of the Aurora-A kinase, promotes cancer cell growth through WNT/TCF-4 signaling activation. (PMID:22258766)
- the histone sequence is recognized in a distinct manner involving the amino terminus and a pair of arginine residues of histone H3, and disruption of the binding impaired stimulation of pre-RNA expression by Spindlin1 (PMID:23077255)
- Spindlin1 activates Wnt/beta-catenin signaling downstream from protein arginine methyltransferase 2 (PRMT2) and the MLL complex, which together are capable of generating a specific H3 “K4me3-R8me2a” pattern. (PMID:24589551)
- High SPIN1 expression is associated with liposarcoma. (PMID:25749382)
- These data indicate that miR-489 could reverse the chemoresistance of breast cancer via the PI3K-Akt pathway by targeting SPIN1. (PMID:27171498)
- In triple negative breast cancer (TNBC) cells the simultaneous miR-29b-1-5p down regulation and SPIN1 up-regulation can potentially be associated with TNBC malignancy. (PMID:28423652)
- miR-489 loss facilitates malignant phenotype of glioma cells probably via SPIN1-mediated PI3K/AKT pathway. (PMID:28666210)
- identified SPIN.DOC as a transcriptional repressor that binds SPIN1 and masks its ability to engage the H3-Lys-4 trimethylation activation mark (PMID:29061846)
- we describe the first example of a histone code reader controlling SkM development in mice, which hints at Spin1 as a potential player in human SkM disease. (PMID:29168801)
- miR-1271 can suppress breast cancer cell proliferation and progression by targeting SPIN1 expression (PMID:29771421)
- Results identified a nucleolar localization signal in the N-terminal region of spindlin 1 (SPIN1) that is essential for its enrichment and function in the nucleolus. (PMID:30249398)
- Data show that spindlin-1 binds methylated arginine R23 of histone H4 (H4), suggsting the function of Spindlin-1. (PMID:30381828)
- SPINDOC Interacts with SPIN1 in DNA independent manner. SPINDOC regulates expression and chromatin loading of SPIN1. (PMID:30803761)
- LINC00473/miR-374a-5p regulates esophageal squamous cell carcinoma via targeting SPIN1 to weaken the effect of radiotherapy. (PMID:31017716)
- SPIN1 triggers abnormal lipid metabolism and enhances tumor growth in liver cancer. (PMID:31790762)
- E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer. (PMID:32767629)
- Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis. (PMID:32940043)
- Long noncoding RNA PSMA3AS1 functions as a microRNA4093p sponge to promote the progression of nonsmall cell lung carcinoma by targeting spindlin 1. (PMID:32945481)
- Structure-Based Design, Docking and Binding Free Energy Calculations of A366 Derivatives as Spindlin1 Inhibitors. (PMID:34072837)
- The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/beta-catenin pathway. (PMID:34753384)
- Recognition of Dimethylarginine Analogues by Tandem Tudor Domain Protein Spindlin1. (PMID:35164245)
- Epigenetic Control of Cancer Cell Proliferation and Cell Cycle Progression by HNRNPK via Promoting Exon 4 Inclusion of Histone Code Reader SPIN1. (PMID:36736887)
- Molecular Basis for SPINDOC-Spindlin1 Engagement and Its Role in Transcriptional Attenuation. (PMID:37977297)
- SPIN1 facilitates chemoresistance and HR repair by promoting Tip60 binding to H3K9me3. (PMID:39090319)
Cross-species orthologs
6 orthologs
| Organism | Symbol | Gene ID |
|---|---|---|
| danio_rerio | spinb | ENSDARG00000035697 |
| danio_rerio | spina | ENSDARG00000058949 |
| mus_musculus | Spin1 | ENSMUSG00000021395 |
| rattus_norvegicus | Spin1 | ENSRNOG00000031378 |
| rattus_norvegicus | Spin1 | ENSRNOG00000064787 |
| rattus_norvegicus | ENSRNOG00000089199 |
Paralogs (4): SPIN2A (ENSG00000147059), SPIN4 (ENSG00000186767), SPIN2B (ENSG00000186787), SPIN3 (ENSG00000204271)
Protein
Protein identifiers
Spindlin-1 — Q9Y657 (reviewed: Q9Y657)
Alternative names: Ovarian cancer-related protein, Spindlin1
All UniProt accessions (1): Q9Y657
UniProt curated annotations — full annotation on UniProt →
Function. Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at ‘Lys-4’ and ‘Lys-9’ (H3K4me3K9me3) and is involved in piRNA-mediated retrotransposon silencing during spermatogenesis. Plays a key role in the initiation of the PIWIL4-piRNA pathway, a pathway that directs transposon DNA methylation and silencing in the male embryonic germ cells, by promoting recruitment of DNA methylation machinery to transposons: binds young, but not old, LINE1 transposons, which are specifically marked with H3K4me3K9me3, and promotes the recruitment of PIWIL4 and SPOCD1 to transposons, leading to piRNA-directed DNA methylation. Also recognizes and binds histone H3 both trimethylated at ‘Lys-4’ and asymmetrically dimethylated at ‘Arg-8’ (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway. Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes. May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.
Subunit / interactions. Homodimer; may form higher-order oligomers. Interacts with TCF7L2/TCF4; the interaction is direct. Interacts with HABP4 and SERBP1. Interacts with SPINDOC; SPINDOC stabilizes SPIN1 and enhances its association with bivalent H3K4me3K9me3 mark. Interacts with SPOCD1; promoting recruitment of PIWIL4 and SPOCD1 to transposons.
Subcellular location. Nucleus. Nucleolus.
Tissue specificity. Highly expressed in ovarian cancer tissues.
Post-translational modifications. Phosphorylated during oocyte meiotic maturation.
Domain organisation. The 3 tudor-like domains (also named Spin/Ssty repeats) specifically recognize and bind methylated histones. H3K4me3 and H3R8me2a are recognized by tudor-like domains 2 and 1, respectively.
Similarity. Belongs to the SPIN/STSY family.
RefSeq proteins (1): NP_006708* (*=MANE)
Domains & families (InterPro)
| ID | Name | Type |
|---|---|---|
| IPR003671 | SPIN/Ssty | Family |
| IPR042567 | SPIN/Ssty_sf | Homologous_superfamily |
Pfam: PF02513
UniProt features (59 total): strand 19, mutagenesis site 14, region of interest 7, modified residue 4, site 3, cross-link 3, helix 3, turn 2, chain 1, sequence variant 1, sequence conflict 1, compositionally biased region 1
Structure
Experimental structures (PDB)
20 structures.
| PDB | Method | Resolution (Å) |
|---|---|---|
| 7OCB | X-RAY DIFFRACTION | 1.42 |
| 6I8Y | X-RAY DIFFRACTION | 1.52 |
| 6I8L | X-RAY DIFFRACTION | 1.58 |
| 6QPL | X-RAY DIFFRACTION | 1.6 |
| 7CNA | X-RAY DIFFRACTION | 1.6 |
| 4MZG | X-RAY DIFFRACTION | 1.7 |
| 6I8B | X-RAY DIFFRACTION | 1.76 |
| 8GTX | X-RAY DIFFRACTION | 1.8 |
| 9T2Z | X-RAY DIFFRACTION | 1.87 |
| 4H75 | X-RAY DIFFRACTION | 2.1 |
| 4MZF | X-RAY DIFFRACTION | 2.1 |
| 2NS2 | X-RAY DIFFRACTION | 2.2 |
| 4MZH | X-RAY DIFFRACTION | 2.2 |
| 5JSJ | X-RAY DIFFRACTION | 2.35 |
| 5JSG | X-RAY DIFFRACTION | 2.5 |
| 7E9M | X-RAY DIFFRACTION | 2.5 |
| 7EA1 | X-RAY DIFFRACTION | 2.7 |
| 7BQZ | X-RAY DIFFRACTION | 3.1 |
| 5Y5W | X-RAY DIFFRACTION | 3.3 |
| 7BU9 | X-RAY DIFFRACTION | 3.5 |
Predicted structure (AlphaFold)
| Model | pLDDT | Fraction very-high |
|---|---|---|
| AF-Q9Y657-F1 | 82.14 | 0.67 |
Functional residue map
Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.
Catalytic / active sites (3): 180 (histone h3k4me3 and h3r8me2a binding); 184 (histone h3k4me3 and h3r8me2a binding); 173 (histone h3k4me3 and h3r8me2a binding)
Post-translational modifications (7): 44, 109, 124, 199, 7, 28, 44
Mutagenesis-validated functional residues (14):
| Position | Phenotype |
|---|---|
| 62 | decreased binding to histone h3 trimethylated at both ’lys-4’ and ’lys-9’ (h3k4me3k9me3). |
| 72 | impaired binding to histone h3k4me3 and h3r8me2a and impaired ability to activate the wnt signaling pathway. ecreased bi |
| 91 | decreased binding to histone h3 trimethylated at both ’lys-4’ and ’lys-9’ (h3k4me3k9me3). |
| 98 | decreased binding to histone h3 trimethylated at both ’lys-4’ and ’lys-9’ (h3k4me3k9me3). |
| 98 | impaired binding to histone h3k4me3 and h3r8me2a and impaired ability to activate the wnt signaling pathway. |
| 109 | impaired phosphorylation. |
| 124 | impaired phosphorylation. |
| 141 | impaired binding to histone h3k4me3 and h3r8me2a and impaired ability to activate the wnt signaling pathway. impaired ab |
| 142 | impaired binding to histone h3k4me3 and h3r8me2a. |
| 170 | impaired binding to histone h3k4me3 and h3r8me2a and impaired ability to activate the wnt signaling pathway. impaired ab |
| 177 | impaired binding to histone h3k4me3 and h3r8me2a. |
| 184 | impaired binding to histone h3k4me3 and h3r8me2a. |
| 189 | impaired binding to histone h3k4me3. |
| 251 | impaired binding to histone h3k4me3 and h3r8me2a and impaired ability to activate the wnt signaling pathway. |
Function
Pathways and Gene Ontology
Reactome pathways
0 pathways
MSigDB gene sets: 242 (showing top):
TTTGTAG_MIR520D, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_DN, MCBRYAN_PUBERTAL_TGFB1_TARGETS_UP, GOBP_REGULATION_OF_WNT_SIGNALING_PATHWAY, GOBP_RRNA_TRANSCRIPTION, STARK_HYPPOCAMPUS_22Q11_DELETION_UP, MARTINEZ_RB1_TARGETS_UP, GOBP_NEGATIVE_REGULATION_OF_GENE_EXPRESSION_EPIGENETIC, GOBP_DNA_METHYLATION_DEPENDENT_CONSTITUTIVE_HETEROCHROMATIN_FORMATION, CREIGHTON_ENDOCRINE_THERAPY_RESISTANCE_1, GOBP_PROTEIN_LOCALIZATION_TO_CHROMATIN, GOBP_PROTEIN_LOCALIZATION_TO_CHROMOSOME, MCBRYAN_PUBERTAL_BREAST_5_6WK_UP, DODD_NASOPHARYNGEAL_CARCINOMA_UP
GO Biological Process (10): regulation of DNA-templated transcription (GO:0006355), gamete generation (GO:0007276), rRNA transcription (GO:0009303), Wnt signaling pathway (GO:0016055), positive regulation of Wnt signaling pathway (GO:0030177), positive regulation of DNA-templated transcription (GO:0045893), meiotic cell cycle (GO:0051321), protein localization to chromatin (GO:0071168), transposable element silencing by piRNA-mediated DNA methylation (GO:0141196), chromatin organization (GO:0006325)
GO Molecular Function (3): histone H3K4me3 reader activity (GO:0140002), histone reader activity (GO:0140566), protein binding (GO:0005515)
GO Cellular Component (7): chromatin (GO:0000785), nucleus (GO:0005634), nucleoplasm (GO:0005654), nucleolus (GO:0005730), spindle (GO:0005819), cytosol (GO:0005829), nuclear membrane (GO:0031965)
GO top-level categories
Rollup of top GO terms by namespace:
| Category | Terms |
|---|---|
| DNA-templated transcription | 3 |
| cellular anatomical structure | 3 |
| sexual reproduction | 2 |
| nuclear lumen | 2 |
| intracellular membraneless organelle | 2 |
| regulation of gene expression | 1 |
| regulation of RNA biosynthetic process | 1 |
| multicellular organismal reproductive process | 1 |
| rRNA metabolic process | 1 |
| cell surface receptor signaling pathway | 1 |
| positive regulation of signal transduction | 1 |
| Wnt signaling pathway | 1 |
| regulation of Wnt signaling pathway | 1 |
| regulation of DNA-templated transcription | 1 |
| positive regulation of RNA biosynthetic process | 1 |
| cell cycle | 1 |
| reproductive process | 1 |
| meiotic nuclear division | 1 |
| protein localization to chromosome | 1 |
| transposable element silencing by heterochromatin formation | 1 |
| gene silencing by piRNA-directed DNA methylation | 1 |
| cellular component organization | 1 |
| histone H3 reader activity | 1 |
| nucleosome | 1 |
| histone binding | 1 |
| chromatin-protein adaptor activity | 1 |
| binding | 1 |
| chromosome | 1 |
| intracellular membrane-bounded organelle | 1 |
| microtubule cytoskeleton | 1 |
| cytoplasm | 1 |
| nucleus | 1 |
| nuclear envelope | 1 |
| organelle membrane | 1 |
Protein interactions and networks
STRING
536 interactions, top by confidence (×1000):
| Protein A | Protein B | Partner UniProt | Score |
|---|---|---|---|
| SPIN1 | SPINDOC | Q9BUA3 | 666 |
| SPIN1 | H3-3A | P06351 | 620 |
| SPIN1 | H3C1 | P02295 | 619 |
| SPIN1 | H3-5 | Q6NXT2 | 618 |
| SPIN1 | H3-7 | Q5TEC6 | 617 |
| SPIN1 | H3C14 | Q71DI3 | 616 |
| SPIN1 | H3-4 | Q16695 | 616 |
| SPIN1 | CPEB1 | Q9BZB8 | 497 |
| SPIN1 | IL3 | P08700 | 496 |
| SPIN1 | SETDB1 | Q15047 | 491 |
| SPIN1 | SGF29 | Q96ES7 | 467 |
| SPIN1 | WDR5 | P61964 | 452 |
| SPIN1 | TAF3 | Q5VWG9 | 426 |
| SPIN1 | KAT7 | O95251 | 413 |
| SPIN1 | PHF23 | Q9BUL5 | 410 |
IntAct
56 interactions, top by confidence:
| A | B | Type | Score |
|---|---|---|---|
| SPIN1 | psi-mi:“MI:0407”(direct interaction) | 0.650 | |
| rep | EIF4E2 | psi-mi:“MI:0914”(association) | 0.640 |
| SPIN1 | SPINDOC | psi-mi:“MI:0914”(association) | 0.640 |
| SPIN1 | psi-mi:“MI:0915”(physical association) | 0.560 | |
| SPINDOC | SPIN3 | psi-mi:“MI:0914”(association) | 0.560 |
| ZNF324B | ZNF316 | psi-mi:“MI:0914”(association) | 0.530 |
| SPIN2B | WDHD1 | psi-mi:“MI:0914”(association) | 0.530 |
| SPIN1 | PAX3 | psi-mi:“MI:0914”(association) | 0.530 |
| CUEDC1 | TOM1 | psi-mi:“MI:0914”(association) | 0.530 |
| ZNF324B | ZNF324 | psi-mi:“MI:0914”(association) | 0.530 |
| HSPB1 | SPIN1 | psi-mi:“MI:0915”(physical association) | 0.510 |
| SPIN1 | HSPB1 | psi-mi:“MI:0915”(physical association) | 0.510 |
| HMGB1 | SPIN1 | psi-mi:“MI:0915”(physical association) | 0.370 |
| SPIN1 | PRMT6 | psi-mi:“MI:0915”(physical association) | 0.370 |
| ARRB1 | psi-mi:“MI:0914”(association) | 0.350 | |
| ARRB2 | psi-mi:“MI:0914”(association) | 0.350 | |
| HSD17B10 | HMGB1P1 | psi-mi:“MI:0914”(association) | 0.350 |
| SOD1 | NPEPPSL1 | psi-mi:“MI:0914”(association) | 0.350 |
| VDAC1 | SNRPGP15 | psi-mi:“MI:0914”(association) | 0.350 |
| AGO3 | PRMT5 | psi-mi:“MI:0914”(association) | 0.350 |
BioGRID (144): SPIN1 (Affinity Capture-MS), SPIN4 (Affinity Capture-MS), SUPT16H (Affinity Capture-MS), BCLAF1 (Affinity Capture-MS), THRAP3 (Affinity Capture-MS), CGGBP1 (Affinity Capture-MS), SSRP1 (Affinity Capture-MS), YRDC (Affinity Capture-MS), C11orf84 (Affinity Capture-MS), MCM2 (Affinity Capture-MS), ZGPAT (Affinity Capture-MS), SF3A3 (Affinity Capture-MS), SCNM1 (Affinity Capture-MS), TOPORS (Affinity Capture-MS), PAX3 (Affinity Capture-MS)
ESM2 similar proteins: A0A8I5ZNK2, A2A432, A9X1D0, B0VX69, B1MTJ4, B2KI88, B5FW36, B7ZUF3, C1FXW2, D2XV59, E1C1R4, F4JL28, O00178, O08582, O70133, O75688, O88506, O95747, P11029, P23727, P26450, P27986, P40692, P60762, Q13085, Q13330, Q4V8J7, Q503N9, Q58DC5, Q5R495, Q5R685, Q5R8Q7, Q5R997, Q5ZML9, Q61142, Q62599, Q63787, Q6P9R2, Q863I2, Q86TU7
Diamond homologs: P13675, Q2KI39, Q4V8J7, Q56A73, Q5JUX0, Q5R997, Q5RA80, Q5RAW7, Q61142, Q6NVE3, Q8K1L2, Q90WG1, Q90WG2, Q99865, Q9BPZ2, Q9Y657
SIGNOR signaling
2 interactions.
| A | Effect | B | Mechanism |
|---|---|---|---|
| AURKA | “up-regulates activity” | SPIN1 | phosphorylation |
Disease & clinical
Clinical variants and AI predictions
ClinVar
22 variants total. Per-class counts are floors (≥ shown; pagination cap):
| Classification | Count (floor) |
|---|---|
| Pathogenic | 0 |
| Likely pathogenic | 0 |
| Uncertain significance | 10 |
| Likely benign | 0 |
| Benign | 0 |
Top pathogenic / likely-pathogenic (0)
SpliceAI
2268 predictions. Top by Δscore:
| Variant | Effect | Δscore |
|---|---|---|
| 9:88388535:GCAG:G | donor_gain | 1.0000 |
| 9:88388536:CAG:C | donor_loss | 1.0000 |
| 9:88388537:AG:A | donor_loss | 1.0000 |
| 9:88388538:GG:G | donor_loss | 1.0000 |
| 9:88388539:GT:G | donor_loss | 1.0000 |
| 9:88388540:T:A | donor_loss | 1.0000 |
| 9:88426379:CAGAG:C | acceptor_loss | 1.0000 |
| 9:88426380:A:AG | acceptor_gain | 1.0000 |
| 9:88426380:AGAGT:A | acceptor_gain | 1.0000 |
| 9:88426381:G:GC | acceptor_loss | 1.0000 |
| 9:88426381:G:GG | acceptor_gain | 1.0000 |
| 9:88426381:GA:G | acceptor_gain | 1.0000 |
| 9:88426381:GAGT:G | acceptor_gain | 1.0000 |
| 9:88426381:GAGTG:G | acceptor_gain | 1.0000 |
| 9:88426590:AGG:A | donor_loss | 1.0000 |
| 9:88426592:G:GA | donor_loss | 1.0000 |
| 9:88426593:T:A | donor_loss | 1.0000 |
| 9:88433681:A:AG | acceptor_gain | 1.0000 |
| 9:88433682:A:G | acceptor_gain | 1.0000 |
| 9:88448935:TTACA:T | acceptor_loss | 1.0000 |
| 9:88448936:TACA:T | acceptor_loss | 1.0000 |
| 9:88448937:ACAGG:A | acceptor_loss | 1.0000 |
| 9:88448938:CA:C | acceptor_loss | 1.0000 |
| 9:88448939:A:AG | acceptor_gain | 1.0000 |
| 9:88448939:A:G | acceptor_loss | 1.0000 |
| 9:88448939:AG:A | acceptor_gain | 1.0000 |
| 9:88448940:G:GA | acceptor_gain | 1.0000 |
| 9:88448940:GG:G | acceptor_gain | 1.0000 |
| 9:88448940:GGC:G | acceptor_gain | 1.0000 |
| 9:88448940:GGCC:G | acceptor_gain | 1.0000 |
AlphaMissense
1736 scored. Top likely-pathogenic:
| Variant | Protein change | am_pathogenicity |
|---|---|---|
| 9:88462567:T:A | I58N | 1.000 |
| 9:88462578:T:A | W62R | 1.000 |
| 9:88462578:T:C | W62R | 1.000 |
| 9:88462580:G:C | W62C | 1.000 |
| 9:88462580:G:T | W62C | 1.000 |
| 9:88462608:T:A | W72R | 1.000 |
| 9:88462608:T:C | W72R | 1.000 |
| 9:88462609:G:C | W72S | 1.000 |
| 9:88462610:G:C | W72C | 1.000 |
| 9:88462610:G:T | W72C | 1.000 |
| 9:88462614:G:A | G74R | 1.000 |
| 9:88462614:G:C | G74R | 1.000 |
| 9:88462615:G:A | G74E | 1.000 |
| 9:88462615:G:T | G74V | 1.000 |
| 9:88462621:T:A | V76D | 1.000 |
| 9:88462624:T:A | L77Q | 1.000 |
| 9:88462624:T:C | L77P | 1.000 |
| 9:88462651:T:C | L86S | 1.000 |
| 9:88462657:T:C | L88P | 1.000 |
| 9:88462660:T:A | I89K | 1.000 |
| 9:88462662:A:G | K90E | 1.000 |
| 9:88462663:A:T | K90I | 1.000 |
| 9:88462664:A:C | K90N | 1.000 |
| 9:88462664:A:T | K90N | 1.000 |
| 9:88462665:T:C | Y91H | 1.000 |
| 9:88462677:G:C | D95H | 1.000 |
| 9:88462677:G:T | D95Y | 1.000 |
| 9:88462678:A:G | D95G | 1.000 |
| 9:88462678:A:T | D95V | 1.000 |
| 9:88462681:G:A | C96Y | 1.000 |
dbSNP variants (sampled 300 via entrez): RS1000076624 (9:88428572 G>A), RS1000084295 (9:88400968 G>A,C), RS1000090219 (9:88393604 T>C), RS1000139185 (9:88459376 T>A,C,G), RS1000163793 (9:88473028 C>G), RS1000206047 (9:88468204 G>A), RS1000213358 (9:88459673 C>T), RS1000215960 (9:88403989 C>T), RS1000232687 (9:88432652 T>G), RS1000339664 (9:88390931 A>G,T), RS1000361098 (9:88433873 A>G), RS1000367905 (9:88389429 C>T), RS1000372594 (9:88456892 A>G), RS1000381651 (9:88442396 T>A,C), RS1000417894 (9:88428293 A>G)
Disease associations
OMIM: gene MIM:609936 | disease phenotypes:
GenCC curated gene-disease
Mondo (0):
Orphanet (0):
HPO phenotypes
0 total (0 of 0 shown, HPO-id order):
GWAS associations
4 associations (top):
| Study | Trait | p-value |
|---|---|---|
| GCST000175_1 | Height | 9.000000e-07 |
| GCST000817_172 | Height | 9.000000e-13 |
| GCST006192_44 | Systolic blood pressure x smoking status (ever vs never) interaction (2df test) | 4.000000e-09 |
| GCST008839_235 | Height | 1.000000e-10 |
EFO canonical traits (2, from GWAS)
| EFO ID | Trait name |
|---|---|
| EFO:0006335 | systolic blood pressure |
| EFO:0006527 | smoking status measurement |
Drugs & pharmacology
Drug and pharmacology data
Is drug target: yes
ChEMBL targets (1): CHEMBL4523509 (SINGLE PROTEIN)
PharmGKB: 1 entry (VIP=true, CPIC=false)
GtoPdb / IUPHAR curated pharmacology
(IUPHAR/BPS Guide to Pharmacology — expert-curated)
Target class: other protein — Methyllysine reader proteins
Most potent curated ligand interactions (2 total), top 2:
| Ligand | Action | Affinity | Parameter |
|---|---|---|---|
| MS8535 | Binding | 7.52 | pKd |
| MS31 | Binding | 7.04 | pKd |
ChEMBL bioactivities
75 potent at pChembl≥5 of 76 total, top 50 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).
PubChem BioAssay actives
75 with measured affinity, of 94 total; 38 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.
| Compound | Assay | Type | Value | Unit |
|---|---|---|---|---|
| 3-[4-[[2-[3-[2-amino-5-(cyclopropylmethoxy)-3,3-dimethylindol-6-yl]oxypropyl]-1,3-dihydroisoindol-5-yl]oxymethyl]triazol-1-yl]-1-[4-(2-pyrrolidin-1-ylethyl)piperidin-1-yl]propan-1-one | 1526499: Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0066 | uM |
| 2-[4-[2-[[2-[3-[2-amino-5-(cyclopropylmethoxy)-3,3-dimethylindol-6-yl]oxypropyl]-1,3-dihydroisoindol-5-yl]oxy]ethyl]triazol-1-yl]-1-[4-(2-pyrrolidin-1-ylethyl)piperidin-1-yl]ethanone | 1526499: Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0099 | uM |
| 3-[4-[2-[[2-[3-[2-amino-5-(cyclopropylmethoxy)-3,3-dimethylindol-6-yl]oxypropyl]-1,3-dihydroisoindol-5-yl]oxy]ethyl]triazol-1-yl]-1-[4-(2-pyrrolidin-1-ylethyl)piperidin-1-yl]propan-1-one | 1526499: Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0101 | uM |
| 5-(cyclopropylmethoxy)-6-[3-(1,3-dihydroisoindol-2-yl)propoxy]-3,3-dimethylindol-2-amine | 1526499: Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0154 | uM |
| 7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-2-N-[2-(dimethylamino)ethyl]-6-methoxy-4-N-(1-propan-2-ylpiperidin-4-yl)quinazoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0180 | uM |
| N-[7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-6-methoxy-4-pyrrolidin-1-ylquinazolin-2-yl]-N’,N’-dimethylethane-1,2-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0300 | uM |
| 7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-2-N-[3-(dimethylamino)propyl]-6-methoxy-4-N-(1-propan-2-ylpiperidin-4-yl)quinoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0340 | uM |
| 2-N-[2-(dimethylamino)ethyl]-6-methoxy-4-N-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0450 | uM |
| 7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-6-methoxy-4-N-(1-propan-2-ylpiperidin-4-yl)-2-N-(2-pyrrolidin-1-ylethyl)quinoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0510 | uM |
| 2-[4-[[2-[3-[2-amino-5-(cyclopropylmethoxy)-3,3-dimethylindol-6-yl]oxypropyl]-1,3-dihydroisoindol-5-yl]oxymethyl]triazol-1-yl]-1-[4-(2-pyrrolidin-1-ylethyl)piperidin-1-yl]ethanone | 1526499: Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0600 | uM |
| 5’-methoxy-6’-(3-pyrrolidin-1-ylpropoxy)spiro[cyclobutane-1,3’-indole]-2’-amine | 1578933: Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay | ic50 | 0.0720 | uM |
| [3-(aminomethyl)-5-[3-(1,3-dihydroisoindol-2-yl)propoxy]-4-methoxyphenyl]methanamine | 1578933: Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay | ic50 | 0.0770 | uM |
| 7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-6-methoxy-2-N-[(1-methylpyrrolidin-2-yl)methyl]-4-N-(1-propan-2-ylpiperidin-4-yl)quinoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0880 | uM |
| 7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-6-methoxy-2-N-(2-piperidin-1-ylethyl)-4-N-(1-propan-2-ylpiperidin-4-yl)quinoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.0920 | uM |
| 6-methoxy-4-N-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.1140 | uM |
| 7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-6-methoxy-2-N-(2-morpholin-4-ylethyl)-4-N-(1-propan-2-ylpiperidin-4-yl)quinoline-2,4-diamine | 2103405: Binding affinity to human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 0.1640 | uM |
| 7-[3-(1,3-dihydroisoindol-2-yl)propoxy]-2-N-[2-(dimethylamino)ethyl]-6-methoxy-4-N-(1-propan-2-ylpiperidin-4-yl)quinoline-2,4-diamine | 2103404: Displacement of FL-H3K4me3 peptide from human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by fluorescence polarization assay | ic50 | 0.3260 | uM |
| [3-(aminomethyl)-4-methoxy-5-(3-pyrrolidin-1-ylpropoxy)phenyl]methanamine | 1578933: Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay | ic50 | 0.3380 | uM |
| 5’-(cyclopropylmethoxy)-6’-[3-(5-methoxy-1,3-dihydroisoindol-2-yl)propoxy]spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 0.3400 | uM |
| 5’-(cyclopropylmethoxy)-6’-[3-(1,3-dihydroisoindol-2-yl)propoxy]spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 0.3900 | uM |
| 5’-methoxy-6’-[3-(5-methoxy-1,3-dihydroisoindol-2-yl)propoxy]spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 0.6000 | uM |
| 6’-[3-(1,3-dihydroisoindol-2-yl)propoxy]-5’-methoxyspiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 0.6600 | uM |
| 5’-methoxy-6’-(3-pyrrolidin-1-ylpropoxy)spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 0.7600 | uM |
| 6-methoxy-4-pyrrolidin-1-yl-7-(3-pyrrolidin-1-ylpropoxy)quinazolin-2-amine | 2103404: Displacement of FL-H3K4me3 peptide from human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by fluorescence polarization assay | ic50 | 0.8380 | uM |
| 5’-(cyclopropylmethoxy)-6’-(3-pyrrolidin-1-ylpropoxy)spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 1.1200 | uM |
| 2-[4-[[2-[4-[2-amino-5-(cyclopropylmethoxy)-3,3-dimethylindol-6-yl]oxybutyl]-1,3-dihydroisoindol-5-yl]oxymethyl]triazol-1-yl]-1-[4-(2-pyrrolidin-1-ylethyl)piperidin-1-yl]ethanone | 1526499: Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | kd | 1.3000 | uM |
| 6-methoxy-4-piperidin-1-yl-7-(3-pyrrolidin-1-ylpropoxy)quinazolin-2-amine | 2103404: Displacement of FL-H3K4me3 peptide from human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by fluorescence polarization assay | ic50 | 1.3200 | uM |
| 4-N-cyclohexyl-6-methoxy-4-N-methyl-7-(3-pyrrolidin-1-ylpropoxy)quinazoline-2,4-diamine | 2103404: Displacement of FL-H3K4me3 peptide from human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by fluorescence polarization assay | ic50 | 1.7400 | uM |
| 6-methoxy-2-N-methyl-4-N-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazoline-2,4-diamine | 2103404: Displacement of FL-H3K4me3 peptide from human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by fluorescence polarization assay | ic50 | 2.0600 | uM |
| 5’-ethoxy-6’-(3-pyrrolidin-1-ylpropoxy)spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 2.1500 | uM |
| [4-[4-(2-pyrrolidin-1-ylethyl)piperidine-1-carbonyl]-3-[4-(pyrrolidin-1-ylmethoxy)anilino]phenyl]-[4-(2-pyrrolidin-1-ylethyl)piperidin-1-yl]methanone | 1578926: Inhibition of SPIN1 (unknown origin) assessed as dissociation constant by isothermal titration calorimetry | kd | 3.0000 | uM |
| 2-cyclohexyl-6-methoxy-N-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazolin-4-amine | 1578933: Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay | ic50 | 3.2000 | uM |
| 6-methoxy-4-[(1-propan-2-ylpiperidin-4-yl)amino]-7-(3-pyrrolidin-1-ylpropoxy)-1H-quinazolin-2-one | 2103404: Displacement of FL-H3K4me3 peptide from human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by fluorescence polarization assay | ic50 | 3.9900 | uM |
| 5’-(cyclopropylmethoxy)-6’-[4-(1,3-dihydroisoindol-2-yl)butoxy]spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 6.2200 | uM |
| 5’-methoxy-6’-[3-(4-methoxy-1,3-dihydroisoindol-2-yl)propoxy]spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 6.6500 | uM |
| 5-(cyclopropylmethoxy)-6-[4-(1,3-dihydroisoindol-2-yl)butoxy]-3,3-dimethylindol-2-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 6.9200 | uM |
| 6-methoxy-2-N,2-N-dimethyl-4-N-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazoline-2,4-diamine | 2103404: Displacement of FL-H3K4me3 peptide from human N-terminal His10 tagged SPIN1 (G21 to S262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by fluorescence polarization assay | ic50 | 7.9700 | uM |
| 5’-propan-2-yloxy-6’-(3-pyrrolidin-1-ylpropoxy)spiro[cyclopentane-1,3’-indole]-2’-amine | 1526494: Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | ic50 | 8.9100 | uM |
CTD chemical–gene interactions
37 total (human), top 30 by PubMed support.
| Chemical | Actions (top 5) | PubMed papers |
|---|---|---|
| Benzo(a)pyrene | affects methylation, decreases methylation | 2 |
| Aflatoxin B1 | increases methylation | 2 |
| aristolochic acid I | decreases expression | 1 |
| bisphenol F | affects cotreatment, decreases expression | 1 |
| TAK-243 | affects sumoylation | 1 |
| triphenyl phosphate | affects expression | 1 |
| alpha-pinene | affects cotreatment, increases oxidation, increases abundance | 1 |
| bisphenol A | affects cotreatment, increases methylation | 1 |
| arsenite | affects binding, decreases reaction | 1 |
| benzo(e)pyrene | increases methylation | 1 |
| aflatoxin B2 | decreases methylation | 1 |
| methacrylaldehyde | increases abundance, affects cotreatment, increases oxidation | 1 |
| ICG 001 | increases expression | 1 |
| abrine | decreases expression | 1 |
| 3-(2-hydroxy-4-(2-methylnonan-2-yl)phenyl)cyclohexan-1-ol | decreases expression | 1 |
| 4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid | increases expression | 1 |
| Fulvestrant | affects cotreatment, increases methylation | 1 |
| Vorinostat | decreases expression | 1 |
| Acrolein | affects cotreatment, increases oxidation, increases abundance | 1 |
| Air Pollutants | increases abundance, increases oxidation, affects cotreatment | 1 |
| Dexamethasone | affects cotreatment, decreases expression | 1 |
| Endosulfan | decreases expression | 1 |
| Hydrogen Peroxide | affects expression | 1 |
| Indomethacin | affects cotreatment, decreases expression | 1 |
| Methapyrilene | increases methylation | 1 |
| Ozone | increases oxidation, increases abundance, affects cotreatment | 1 |
| Rotenone | increases expression | 1 |
| Thiram | decreases expression | 1 |
| Tretinoin | decreases expression | 1 |
| Valproic Acid | affects expression | 1 |
ChEMBL screening assays
18 unique, capped per target: 18 binding
Representative assays (with source publication via chembl_document):
| Assay ID | Type | Description | Source paper |
|---|---|---|---|
| CHEMBL4323086 | Binding | Binding affinity to recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as melting temperature at 20 uM by Sypro orange dye based differential scanning fluorimetry | A Chemical Probe for Tudor Domain Protein Spindlin1 to Investigate Chromatin Function. — J Med Chem |
Clinical trials (associated diseases)
0 trials via MONDO — disease-level, not drug-specific.
Related Atlas pages
No linked Atlas pages yet — the cross-entity mesh grows as the corpus expands.