SSR4

Summary

SSR4 (signal sequence receptor subunit 4, HGNC:11326) is a protein-coding gene on chromosome Xq28, encoding Translocon-associated protein subunit delta (P51571). TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.

This gene encodes the delta subunit of the translocon-associated protein complex which is involved in translocating proteins across the endoplasmic reticulum membrane. The encoded protein is located in the Xq28 region and is arranged in a compact head-to-head manner with the isocitrate dehydrogenase 3 (NAD+) gamma gene and both genes are driven by a CpG-embedded bidirectional promoter. Alternate splicing results in multiple transcript variants.

Source: NCBI Gene 6748 — RefSeq curated summary.

At a glance

• Gene–disease (curated): SSR4-congenital disorder of glycosylation (Strong, GenCC)
• Clinical variants (ClinVar): 181 total — 12 pathogenic, 6 likely-pathogenic
• Phenotypes (HPO): 36
• Druggable target: yes
• MANE Select transcript: NM_006280

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:11326
Approved symbol	SSR4
Name	signal sequence receptor subunit 4
Location	Xq28
Locus type	gene with protein product
Status	Approved
Aliases	TRAPD
Ensembl gene	ENSG00000180879
Ensembl biotype	protein_coding
OMIM	300090
Entrez	6748

Gene structure

Transcript identifiers

Ensembl transcripts: 18 — 10 protein_coding, 7 protein_coding_CDS_not_defined, 1 retained_intron

ENST00000320857, ENST00000370085, ENST00000370086, ENST00000370087, ENST00000447375, ENST00000460616, ENST00000471724, ENST00000471880, ENST00000482902, ENST00000485612, ENST00000486204, ENST00000491833, ENST00000854607, ENST00000854608, ENST00000854609, ENST00000939443, ENST00000939444, ENST00000969382

RefSeq mRNA: 3 — MANE Select: NM_006280 NM_001204526, NM_001204527, NM_006280

CCDS: CCDS14731

Canonical transcript exons

ENST00000370086 — 6 exons

Exon	Start	End
ENSE00001451715	153794668	153794754
ENSE00001915160	153798329	153798499
ENSE00003571167	153797725	153797814
ENSE00003591794	153796434	153796552
ENSE00003607970	153797458	153797532
ENSE00003707581	153798071	153798136

Expression profiles

Bgee: expression breadth ubiquitous, 295 present calls, max score 99.69.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 395.9922 / max 6254.3090, expressed in 1828 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
198106	394.5811	1828
198104	1.4110	904

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
pituitary gland	UBERON:0000007	99.69	gold quality
adenohypophysis	UBERON:0002196	99.67	gold quality
body of pancreas	UBERON:0001150	99.62	gold quality
type B pancreatic cell	CL:0000169	99.57	gold quality
seminal vesicle	UBERON:0000998	99.55	gold quality
trachea	UBERON:0003126	99.52	gold quality
pylorus	UBERON:0001166	99.42	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	99.40	gold quality
saliva-secreting gland	UBERON:0001044	99.38	gold quality
minor salivary gland	UBERON:0001830	99.38	gold quality
pancreas	UBERON:0001264	99.35	gold quality
mucosa of transverse colon	UBERON:0004991	99.32	gold quality
corpus epididymis	UBERON:0004359	99.31	gold quality
stromal cell of endometrium	CL:0002255	99.28	gold quality
islet of Langerhans	UBERON:0000006	99.27	gold quality
body of stomach	UBERON:0001161	99.27	gold quality
endocervix	UBERON:0000458	99.25	gold quality
spleen	UBERON:0002106	99.24	gold quality
left adrenal gland	UBERON:0001234	99.23	gold quality
periodontal ligament	UBERON:0008266	99.23	gold quality
mouth mucosa	UBERON:0003729	99.21	gold quality
nasal cavity mucosa	UBERON:0001826	99.20	gold quality
left adrenal gland cortex	UBERON:0035825	99.20	gold quality
right adrenal gland	UBERON:0001233	99.18	gold quality
bone marrow cell	CL:0002092	99.17	gold quality
epithelium of nasopharynx	UBERON:0001951	99.17	gold quality
right adrenal gland cortex	UBERON:0035827	99.13	gold quality
left coronary artery	UBERON:0001626	99.12	gold quality
granulocyte	CL:0000094	99.11	gold quality
right coronary artery	UBERON:0001625	99.11	gold quality

Single-cell (SCXA)

Detected in 35 experiment(s), a significant marker in 31.

Experiment	Marker?	Max mean expression
E-CURD-126	yes	5380.83
E-MTAB-6653	yes	4952.50
E-HCAD-15	yes	4864.08
E-MTAB-10432	yes	4290.67
E-CURD-46	yes	4217.89
E-CURD-88	yes	3645.61
E-MTAB-6308	yes	3641.26
E-MTAB-8410	yes	3423.26
E-HCAD-4	yes	3255.67
E-MTAB-8322	yes	3187.86
E-HCAD-36	yes	2832.48
E-GEOD-135922	yes	2736.98
E-GEOD-125970	yes	2629.46
E-HCAD-1	yes	2531.12
E-HCAD-11	yes	2482.85

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 3)

• results of this study implicate TRAPD as a candidate gene with potential functions that might be associated with ultraviolet-induced melanomagenesis and metastasis (PMID:15057039)
• We now report eight affected males with either de novo (4) or inherited (4) loss of function mutations in SSR4. (PMID:26264460)
• Expanding the phenotype of X-linked SSR4-CDG: Connective tissue implications. (PMID:33300232)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	ssr4	ENSDARG00000019444
mus_musculus	Ssr4	ENSMUSG00000002014
rattus_norvegicus	Ssr4	ENSRNOG00000053172
drosophila_melanogaster	Tapdelta	FBGN0021795
caenorhabditis_elegans		WBGENE00013238

Protein

Protein identifiers

Translocon-associated protein subunit delta — P51571 (reviewed: P51571)

Alternative names: Signal sequence receptor subunit delta

All UniProt accessions (2): A6NLM8, P51571

UniProt curated annotations — full annotation on UniProt →

Function. TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.

Subunit / interactions. Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.

Subcellular location. Endoplasmic reticulum membrane.

Disease relevance. Congenital disorder of glycosylation 1Y (CDG1Y) [MIM:300934] A form of congenital disorder of glycosylation, a multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. The disease is caused by variants affecting the gene represented in this entry.

Similarity. Belongs to the TRAP-delta family.

RefSeq proteins (3): NP_001191455, NP_001191456, NP_006271* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR008855	TRAP-delta	Family

Pfam: PF05404

UniProt features (9 total): topological domain 2, signal peptide 1, chain 1, transmembrane region 1, disulfide bond 1, cross-link 1, sequence variant 1, sequence conflict 1

Structure

Experimental structures (PDB)

3 structures.

PDB	Method	Resolution (Å)
9N9J	ELECTRON MICROSCOPY	3.2
9YGY	ELECTRON MICROSCOPY	4.1
8B6L	ELECTRON MICROSCOPY	7.6

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P51571-F1	89.09	0.62

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 73

Disulfide bonds (1): 26–57

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-1799339	SRP-dependent cotranslational protein targeting to membrane
R-HSA-392499	Metabolism of proteins
R-HSA-72766	Translation

MSigDB gene sets: 248 (showing top): GSE18804_SPLEEN_MACROPHAGE_VS_BRAIN_TUMORAL_MACROPHAGE_UP, GSE18804_BRAIN_VS_COLON_TUMORAL_MACROPHAGE_UP, GRUETZMANN_PANCREATIC_CANCER_DN, MCLACHLAN_DENTAL_CARIES_UP, GOBP_INTRACELLULAR_PROTEIN_TRANSPORT, XU_GH1_AUTOCRINE_TARGETS_UP, ENK_UV_RESPONSE_KERATINOCYTE_UP, STARK_PREFRONTAL_CORTEX_22Q11_DELETION_DN, MATTIOLI_MGUS_VS_PCL, GOBP_PROTEIN_TARGETING, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ENDOPLASMIC_RETICULUM, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ORGANELLE, GOBP_PROTEIN_TARGETING_TO_MEMBRANE, WTGAAAT_UNKNOWN, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_MEMBRANE

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (6): endoplasmic reticulum (GO:0005783), Sec61 translocon complex (GO:0005784), endomembrane system (GO:0012505), extracellular exosome (GO:0070062), endoplasmic reticulum membrane (GO:0005789), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Translation	1
Metabolism of proteins	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
translocon complex	1
vacuole	1
plasma membrane	1
extracellular vesicle	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1

Protein interactions and networks

STRING

1426 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
SSR4	SSR1	P43307	978
SSR4	SSR3	Q9UNL2	978
SSR4	SSR2	P43308	960
SSR4	HECW1	Q76N89	887
SSR4	IDH3G	P51553	825
SSR4	DDOST	P39656	701
SSR4	IDH2	P48735	638
SSR4	IDH1	O75874	558
SSR4	DERL1	Q9BUN8	524
SSR4	STT3A	P46977	517
SSR4	DVL1	O14640	512
SSR4	STT3B	Q8TCJ2	508
SSR4	SOD1	P00441	480
SSR4	PPIB	P23284	480
SSR4	KRTCAP2	Q8N6L1	476

IntAct

172 interactions, top by confidence:

A	B	Type	Score
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
IFT27	IFT56	psi-mi:“MI:0914”(association)	0.690
ILK	HAX1	psi-mi:“MI:0914”(association)	0.530
EGFR	NDUFA4	psi-mi:“MI:0914”(association)	0.530
RPN1	APBB1	psi-mi:“MI:0914”(association)	0.530
FLVCR1	TNFRSF10B	psi-mi:“MI:0914”(association)	0.530
env	PGRMC1	psi-mi:“MI:0914”(association)	0.460
	GNAT3	psi-mi:“MI:0915”(physical association)	0.400
GPC1	GANAB	psi-mi:“MI:0915”(physical association)	0.400
SSR1	SSR4	psi-mi:“MI:0915”(physical association)	0.400
	SSR4	psi-mi:“MI:0915”(physical association)	0.370
NLGN3	SSR4	psi-mi:“MI:0915”(physical association)	0.370
AATK	DPM1	psi-mi:“MI:0914”(association)	0.350
OTUB1		psi-mi:“MI:0914”(association)	0.350
OTUB1	EPM2A	psi-mi:“MI:0914”(association)	0.350
MAD2L2	DCTN3	psi-mi:“MI:0914”(association)	0.350
TUBA1C	TCP11L2	psi-mi:“MI:0914”(association)	0.350
JUN		psi-mi:“MI:0914”(association)	0.350
JUN	TPM3	psi-mi:“MI:0914”(association)	0.350
		psi-mi:“MI:0914”(association)	0.350
	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (293): SSR4 (Affinity Capture-MS), SSR4 (Affinity Capture-MS), SSR4 (Affinity Capture-MS), SSR4 (Affinity Capture-MS), SSR4 (Affinity Capture-MS), SSR4 (Affinity Capture-MS), ARL8B (Co-fractionation), IPO9 (Co-fractionation), KDSR (Co-fractionation), MAGT1 (Co-fractionation), NCLN (Co-fractionation), RPN2 (Co-fractionation), SSR4 (Co-fractionation), SSR4 (Co-fractionation), SSR4 (Co-fractionation)

ESM2 similar proteins: A0A1L8FM16, A0A1L8H814, A2BD92, A4FUD4, A7E2V1, B0BN86, B1AUE5, F1QR43, O00623, O88177, O95870, P17152, P51571, Q07984, Q08CZ0, Q0VEJ0, Q1JPD2, Q28DH9, Q3B8H3, Q3ZC98, Q3ZT31, Q4QQS8, Q4QRH7, Q4R8P0, Q58E26, Q5R6S0, Q5R822, Q5RBY5, Q5RCP7, Q5REH6, Q62186, Q66HC5, Q6AXN4, Q6DDX8, Q6DFK1, Q6DFS0, Q6IQC7, Q6MG55, Q7SZC5, Q8BJ71

Diamond homologs: P51571, Q07984, Q2TBX5, Q5REH6, Q62186

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
SSR4	“form complex”	“TRAP complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 189 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
PD-L1(CD274) glycosylation and translocation to plasma membrane	6	23.2×	3e-05
Maturation of spike protein	9	17.8×	5e-07
Regulation of CDH1 posttranslational processing and trafficking to plasma membrane	7	17.6×	3e-05
FCERI mediated MAPK activation	5	12.9×	2e-03
Maturation of DENV proteins	8	12.6×	3e-05
SRP-dependent cotranslational protein targeting to membrane	14	10.5×	4e-08
Regulation of RAS by GAPs	7	10.1×	6e-04
Negative regulation of MAPK pathway	5	9.9×	6e-03

GO biological processes:

GO term	Partners	Fold	FDR
obsolete protein N-linked glycosylation via asparagine	5	19.7×	2e-03
extrinsic apoptotic signaling pathway via death domain receptors	6	14.1×	2e-03
protein N-linked glycosylation	6	9.2×	9e-03
cytoplasmic translation	8	8.7×	2e-03
translation	10	6.0×	2e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

181 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	12
Likely pathogenic	6
Uncertain significance	54
Likely benign	42
Benign	12

Top pathogenic / likely-pathogenic (18)

Variant ID	HGVS	Classification
1164036	NM_006280.3(SSR4):c.261+2_261+8del	Pathogenic
1333191	NM_006280.3(SSR4):c.269G>A (p.Trp90Ter)	Pathogenic
1452967	NM_006280.3(SSR4):c.184C>T (p.Gln62Ter)	Pathogenic
209110	NM_006280.3(SSR4):c.317del (p.Phe106fs)	Pathogenic
2441661	NM_006280.3(SSR4):c.270G>A (p.Trp90Ter)	Pathogenic
3392483	NM_006280.3(SSR4):c.68-2A>G	Pathogenic
372143	NM_006280.3(SSR4):c.187-301_352-15del	Pathogenic
372146	NM_006280.3(SSR4):c.418-1G>A	Pathogenic
4759492	NM_006280.3(SSR4):c.68-322_262-46del	Pathogenic
976747	NM_006280.3(SSR4):c.141dup (p.Val48fs)	Pathogenic
984389	NM_006280.3(SSR4):c.241C>T (p.Gln81Ter)	Pathogenic
986726	NM_001204526.1:c.1_185del	Pathogenic
1308630	NM_006280.3(SSR4):c.418-2A>G	Likely pathogenic
2347022	NM_006280.3(SSR4):c.67+2T>C	Likely pathogenic
2572630	NM_006280.3(SSR4):c.235C>T (p.Arg79Ter)	Likely pathogenic
372144	NM_006280.3(SSR4):c.358_359del (p.Arg120fs)	Likely pathogenic
3893303	NM_001204526.2(SSR4):c.19+2T>C	Likely pathogenic
4292876	NM_006280.3(SSR4):c.98del (p.Pro33fs)	Likely pathogenic

SpliceAI

1356 predictions. Top by Δscore:

Variant	Effect	Δscore
X:153796429:CGCA:C	acceptor_loss	1.0000
X:153796430:GCA:G	acceptor_loss	1.0000
X:153796431:CAGCC:C	acceptor_loss	1.0000
X:153796432:A:AG	acceptor_gain	1.0000
X:153796432:A:AT	acceptor_loss	1.0000
X:153796432:AGCC:A	acceptor_gain	1.0000
X:153796433:G:GG	acceptor_gain	1.0000
X:153796433:GCC:G	acceptor_gain	1.0000
X:153796433:GCCG:G	acceptor_gain	1.0000
X:153796433:GCCGA:G	acceptor_gain	1.0000
X:153796548:TCC:T	donor_gain	1.0000
X:153796548:TCCAG:T	donor_loss	1.0000
X:153796549:CCAGG:C	donor_loss	1.0000
X:153796550:CAGGT:C	donor_loss	1.0000
X:153796551:AGGT:A	donor_loss	1.0000
X:153796553:G:GG	donor_gain	1.0000
X:153796553:GT:G	donor_loss	1.0000
X:153796554:T:A	donor_loss	1.0000
X:153797452:A:AG	acceptor_gain	1.0000
X:153797453:C:G	acceptor_gain	1.0000
X:153797453:CCCA:C	acceptor_loss	1.0000
X:153797455:CA:C	acceptor_loss	1.0000
X:153797456:A:AG	acceptor_gain	1.0000
X:153797456:AGAAC:A	acceptor_loss	1.0000
X:153797457:G:GG	acceptor_gain	1.0000
X:153797457:GA:G	acceptor_gain	1.0000
X:153797457:GAAC:G	acceptor_gain	1.0000
X:153797457:GAACA:G	acceptor_gain	1.0000
X:153797702:ACTCT:A	acceptor_gain	1.0000
X:153797715:C:G	acceptor_gain	1.0000

AlphaMissense

1121 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
X:153797501:T:A	V77D	1.000
X:153797474:C:A	A68D	0.999
X:153797731:T:A	W90R	0.999
X:153797731:T:C	W90R	0.999
X:153797733:G:C	W90C	0.999
X:153797733:G:T	W90C	0.999
X:153797774:T:A	V104D	0.999
X:153797780:T:C	F106S	0.999
X:153797810:G:C	R116T	0.999
X:153797811:G:C	R116S	0.999
X:153797811:G:T	R116S	0.999
X:153796535:T:A	C57S	0.998
X:153796535:T:C	C57R	0.998
X:153796536:G:A	C57Y	0.998
X:153796536:G:C	C57S	0.998
X:153797468:T:A	L66H	0.998
X:153797473:G:C	A68P	0.998
X:153797531:A:C	Q87P	0.998
X:153797726:T:A	V88E	0.998
X:153797728:T:C	S89P	0.998
X:153797807:T:C	L115P	0.998
X:153798369:C:A	A153D	0.998
X:153796512:T:C	F49S	0.997
X:153796530:T:C	L55P	0.997
X:153797468:T:C	L66P	0.997
X:153797527:T:G	Y86D	0.997
X:153797810:G:T	R116M	0.997
X:153797814:G:C	K117N	0.997
X:153797814:G:T	K117N	0.997
X:153798078:G:C	R120T	0.997

dbSNP variants (sampled 300 via entrez): RS1002025388 (X:153798582 G>A), RS1002542494 (X:153796209 C>T), RS1004370317 (X:153797518 G>T), RS1006455913 (X:153796218 C>T), RS1006747489 (X:153792922 A>G), RS1007233517 (X:153792503 C>A), RS1007821461 (X:153794355 C>A,T), RS1008163661 (X:153794663 T>C), RS1009297588 (X:153798302 G>C), RS1009636223 (X:153798551 G>A), RS1010122202 (X:153792972 T>C), RS1010174408 (X:153793374 G>A,C), RS1011367123 (X:153796944 G>A), RS1012580774 (X:153795264 C>T), RS1013479336 (X:153798611 C>A)

Disease associations

OMIM: gene MIM:300090 | disease phenotypes: MIM:300934

GenCC curated gene-disease

Disease	Classification	Inheritance
SSR4-congenital disorder of glycosylation	Strong	X-linked

Mondo (1): SSR4-congenital disorder of glycosylation (MONDO:0010490)

Orphanet (1): SSR4-CDG (Orphanet:370927)

HPO phenotypes

36 total (30 of 36 shown, HPO-id order):

HPO	Term
HP:0000047	Hypospadias
HP:0000085	Horseshoe kidney
HP:0000154	Wide mouth
HP:0000252	Microcephaly
HP:0000347	Micrognathia
HP:0000400	Macrotia
HP:0000486	Strabismus
HP:0000490	Deeply set eye
HP:0000687	Widely spaced teeth
HP:0000924	Abnormality of the skeletal system
HP:0001249	Intellectual disability
HP:0001250	Seizure
HP:0001252	Hypotonia
HP:0001263	Global developmental delay
HP:0001290	Generalized hypotonia
HP:0001331	Absent septum pellucidum
HP:0001373	Joint dislocation
HP:0001419	X-linked recessive inheritance
HP:0001508	Failure to thrive
HP:0001626	Abnormality of the cardiovascular system
HP:0001643	Patent ductus arteriosus
HP:0001928	Abnormality of coagulation
HP:0001999	Abnormal facial shape
HP:0002013	Vomiting
HP:0002020	Gastroesophageal reflux
HP:0002079	Hypoplasia of the corpus callosum
HP:0002098	Respiratory distress
HP:0002518	Abnormal periventricular white matter morphology
HP:0002650	Scoliosis
HP:0003256	Abnormality of the coagulation cascade

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4295777 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

42 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cyclosporine	increases expression	4
bisphenol A	affects expression, increases expression, decreases reaction, increases abundance	3
Tunicamycin	increases expression	2
Thapsigargin	increases expression	2
bisphenol F	increases expression	1
ginger extract	decreases reaction, increases abundance, increases expression	1
alpha phellandrene	decreases expression	1
beta-lapachone	decreases expression, increases expression	1
arsenite	increases reaction, affects binding	1
sodium arsenite	affects cotreatment, increases abundance, increases expression	1
cobaltous chloride	increases expression	1
manganese chloride	increases expression, affects cotreatment, increases abundance	1
potassium chromate(VI)	decreases expression	1
aflatoxin B2	increases methylation	1
perfluorooctane sulfonic acid	increases expression	1
chloropicrin	affects expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
2-amino-14,16-dimethyloctadecan-3-ol	decreases expression	1
bisphenol S	increases expression	1
LDN 193189	affects cotreatment, decreases expression	1
bisphenol AF	increases expression	1
Temozolomide	increases expression	1
Air Pollutants	increases abundance, decreases expression	1
Arsenic	affects cotreatment, increases abundance, increases expression	1
Cocaine	decreases expression	1
Diuron	decreases expression	1
Homocysteine	affects cotreatment, affects expression	1
Isoniazid	increases expression	1
Ivermectin	decreases expression	1
Manganese	increases abundance, increases expression, affects cotreatment	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4118569	Binding	Binding affinity to SSR4 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay	Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors. — Bioorg Med Chem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3IC	Abcam HEK293T SSR4 KO	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.