STRAP

Summary

STRAP (serine/threonine kinase receptor associated protein, HGNC:30796) is a protein-coding gene on chromosome 12p12.3, encoding Serine-threonine kinase receptor-associated protein (Q9Y3F4). The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs.

Enables RNA binding activity. Involved in maintenance of gastrointestinal epithelium and spliceosomal snRNP assembly. Acts upstream of with a positive effect on negative regulation of transforming growth factor beta receptor signaling pathway. Located in cytosol. Part of SMN complex. Implicated in adenocarcinoma; colorectal carcinoma; large cell carcinoma; lung carcinoma; and squamous cell neoplasm.

Source: NCBI Gene 11171 — automated summary from GO annotations (Alliance of Genome Resources).

At a glance

• Clinical variants (ClinVar): 45 total
• Regional missense constraint (gnomAD): 1 of 5 regions constrained (≈18% of expected missense variation)
• GTEx eGene: eQTL in 25 tissues, sQTL in 1 (q < 0.05)
• Constraint (gnomAD): pLI 0.84 (intermediate), LOEUF 0.57 (moderately constrained)
• MANE Select transcript: NM_007178

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30796
Approved symbol	STRAP
Name	serine/threonine kinase receptor associated protein
Location	12p12.3
Locus type	gene with protein product
Status	Approved
Aliases	UNRIP, pt-wd, MAWD
Ensembl gene	ENSG00000023734
Ensembl biotype	protein_coding
OMIM	605986
Entrez	11171

Gene structure

Transcript identifiers

Ensembl transcripts: 35 — 19 protein_coding, 14 nonsense_mediated_decay, 2 retained_intron

MANE Select: ENST00000419869

Transcript support level (Ensembl TSL; 1 = fully mRNA-supported): TSL 1: 1, TSL 2: 4, TSL 3: 1

ENST00000419869, ENST00000025399, ENST00000536737, ENST00000538718, ENST00000539887, ENST00000541731, ENST00000888770, ENST00000888771, ENST00000927387, ENST00000927388, ENST00000927389, ENST00000927390, ENST00000927391, ENST00000956572, ENST00000991393, ENST00000991394, ENST00001033302, ENST00001033303, ENST00001033306, ENST00001033309, ENST00001033311, ENST00001033313, ENST00001092516, ENST00001092517, ENST00001092518, ENST00001092519, ENST00001092520, ENST00001092521, ENST00001092522, ENST00001092523, ENST00001092524, ENST00001092525, ENST00001092526, ENST00001107118, ENST00001141796

RefSeq mRNA: 1 — MANE Select: NM_007178 NM_007178

CCDS: CCDS8676

Canonical transcript exons

ENST00000419869 — 10 exons

Exon	Start	End
ENSE00000724091	15883541	15883676
ENSE00002297307	15882387	15882819
ENSE00003479597	15897882	15898018
ENSE00003494228	15894047	15894143
ENSE00003537668	15902917	15903478
ENSE00003596444	15900947	15901012
ENSE00003596910	15889928	15890009
ENSE00003602559	15899904	15900053
ENSE00003604550	15890597	15890669
ENSE00003694195	15895359	15895496

Expression profiles

Bgee: expression breadth ubiquitous, 305 present calls, max score 99.52.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 172.2765 / max 1290.3241, expressed in 1828 samples.

Highest expression (Bgee): secondary oocyte, oocyte, corpus epididymis, pons, cauda epididymis.

Genetic regulation across tissues (GTEx eQTL / sQTL)

STRAP is an eGene in GTEx v10 — its expression is genetically regulated (eQTL) in 25 tissues and its splicing (sQTL) in 1 (q < 0.05). Strongest associations first; slope = normalized effect of the lead variant’s alternative allele.

showing 20 of 26 significant tissue × QTL associations:

Tissue	QTL	q-value	Slope	Lead variant
Nerve Tibial	eqtl	2.7e-09	+0.24	12:15821046:T:C
Artery Tibial	eqtl	7.2e-09	+0.24	12:15874060:C:T
Thyroid	eqtl	6.8e-07	-0.35	12:15836444:C:A
Adipose Subcutaneous	eqtl	2.5e-06	+0.24	12:15915510:T:G
Skin Sun Exposed Lower leg	eqtl	1.6e-05	-0.43	12:16003965:G:T
Artery Aorta	eqtl	2.2e-04	+0.27	12:15897577:G:A
Brain Caudate basal ganglia	eqtl	2.2e-04	-0.64	12:15836444:C:A
Cells Cultured fibroblasts	eqtl	4.2e-04	+0.15	12:15764754:A:G
Pancreas	eqtl	2.0e-03	-0.51	12:15542270:C:T
Esophagus Muscularis	eqtl	3.0e-03	+0.15	12:15736221:T:C
Brain Amygdala	eqtl	3.9e-03	-0.75	12:16010500:C:T
Esophagus Gastroesophageal Junction	eqtl	5.2e-03	+0.18	12:15823326:A:G
Brain Cerebellum	eqtl	5.4e-03	-0.52	12:16003965:G:T
Colon Transverse	eqtl	9.1e-03	+0.10	12:15883327:C:G
Spleen	eqtl	9.5e-03	+0.28	12:15923578:C:T
Brain Hippocampus	eqtl	9.5e-03	-0.47	12:16003965:G:T
Testis	eqtl	1.5e-02	-0.51	12:15706089:C:A
Adipose Visceral Omentum	eqtl	1.7e-02	-0.16	12:15550245:A:G
Esophagus Mucosa	eqtl	2.0e-02	-0.23	12:16187125:A:G
Colon Sigmoid	eqtl	2.4e-02	-0.24	12:15172900:G:A

FANTOM5 promoters

3 alternative TSS:

Promoter ID	TPM avg	Samples expressed
124503	167.9111	1828
124504	4.3072	1495
124502	0.0582	17

Top tissues by expression

showing 100 of 305 tissues by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	99.52	gold quality
oocyte	CL:0000023	99.36	gold quality
corpus epididymis	UBERON:0004359	98.99	gold quality
pons	UBERON:0000988	98.81	gold quality
cauda epididymis	UBERON:0004360	98.81	gold quality
type B pancreatic cell	CL:0000169	98.72	gold quality
caput epididymis	UBERON:0004358	98.71	gold quality
adenohypophysis	UBERON:0002196	98.67	gold quality
embryo	UBERON:0000922	98.64	gold quality
ventricular zone	UBERON:0003053	98.59	gold quality
ganglionic eminence	UBERON:0004023	98.57	gold quality
cortical plate	UBERON:0005343	98.55	gold quality
adult organism	UBERON:0007023	98.51	gold quality
pituitary gland	UBERON:0000007	98.49	gold quality
Brodmann (1909) area 46	UBERON:0006483	98.49	gold quality
hypothalamus	UBERON:0001898	98.45	gold quality
amniotic fluid	UBERON:0000173	98.44	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	98.33	gold quality
lateral nuclear group of thalamus	UBERON:0002736	98.32	gold quality
germinal epithelium of ovary	UBERON:0001304	98.31	gold quality
substantia nigra pars compacta	UBERON:0001965	98.31	gold quality
lower lobe of lung	UBERON:0008949	98.30	gold quality
right frontal lobe	UBERON:0002810	98.29	gold quality
Brodmann (1909) area 9	UBERON:0013540	98.29	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	98.23	gold quality
gingiva	UBERON:0001828	98.20	gold quality
gingival epithelium	UBERON:0001949	98.20	gold quality
amygdala	UBERON:0001876	98.16	gold quality
substantia nigra pars reticulata	UBERON:0001966	98.16	gold quality
middle temporal gyrus	UBERON:0002771	98.12	gold quality
oral cavity	UBERON:0000167	98.11	gold quality
orbitofrontal cortex	UBERON:0004167	98.09	gold quality
superior vestibular nucleus	UBERON:0007227	98.08	gold quality
mucosa of sigmoid colon	UBERON:0004993	98.05	gold quality
Ammon’s horn	UBERON:0001954	98.04	gold quality
body of pancreas	UBERON:0001150	98.01	gold quality
nucleus accumbens	UBERON:0001882	98.01	gold quality
caudate nucleus	UBERON:0001873	97.97	gold quality
forebrain	UBERON:0001890	97.97	gold quality
body of tongue	UBERON:0011876	97.97	gold quality
heart right ventricle	UBERON:0002080	97.96	gold quality
tongue squamous epithelium	UBERON:0006919	97.96	gold quality
Brodmann (1909) area 23	UBERON:0013554	97.94	gold quality
putamen	UBERON:0001874	97.93	gold quality
cerebral cortex	UBERON:0000956	97.92	gold quality
epithelium of nasopharynx	UBERON:0001951	97.92	gold quality
postcentral gyrus	UBERON:0002581	97.91	gold quality
nasopharynx	UBERON:0001728	97.90	gold quality
dorsal root ganglion	UBERON:0000044	97.89	gold quality
telencephalon	UBERON:0001893	97.89	gold quality
hair follicle	UBERON:0002073	97.89	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	97.89	gold quality
endometrium	UBERON:0001295	97.87	gold quality
choroid plexus epithelium	UBERON:0003911	97.87	gold quality
right adrenal gland cortex	UBERON:0035827	97.86	gold quality
decidua	UBERON:0002450	97.85	gold quality
cingulate cortex	UBERON:0003027	97.85	gold quality
nephron tubule	UBERON:0001231	97.84	gold quality
islet of Langerhans	UBERON:0000006	97.82	gold quality
brain	UBERON:0000955	97.81	gold quality
stromal cell of endometrium	CL:0002255	97.80	gold quality
central nervous system	UBERON:0001017	97.80	gold quality
right adrenal gland	UBERON:0001233	97.79	gold quality
primary visual cortex	UBERON:0002436	97.79	gold quality
prefrontal cortex	UBERON:0000451	97.78	gold quality
seminal vesicle	UBERON:0000998	97.78	gold quality
temporal lobe	UBERON:0001871	97.78	gold quality
anterior cingulate cortex	UBERON:0009835	97.78	gold quality
parietal lobe	UBERON:0001872	97.77	gold quality
neocortex	UBERON:0001950	97.77	gold quality
smooth muscle tissue	UBERON:0001135	97.76	gold quality
vena cava	UBERON:0004087	97.76	gold quality
lower esophagus	UBERON:0013473	97.75	gold quality
lower esophagus muscularis layer	UBERON:0035833	97.75	gold quality
gluteal muscle	UBERON:0002000	97.74	gold quality
right atrium auricular region	UBERON:0006631	97.74	gold quality
biceps brachii	UBERON:0001507	97.73	gold quality
tongue	UBERON:0001723	97.73	gold quality
mammary duct	UBERON:0001765	97.73	gold quality
tibial artery	UBERON:0007610	97.73	gold quality
popliteal artery	UBERON:0002250	97.72	gold quality
hindlimb stylopod muscle	UBERON:0004252	97.72	gold quality
frontal cortex	UBERON:0001870	97.71	gold quality
mucosa of transverse colon	UBERON:0004991	97.71	gold quality
frontal lobe	UBERON:0016525	97.71	gold quality
diaphragm	UBERON:0001103	97.70	gold quality
left adrenal gland	UBERON:0001234	97.70	gold quality
triceps brachii	UBERON:0001509	97.70	gold quality
occipital lobe	UBERON:0002021	97.70	gold quality
tibia	UBERON:0000979	97.69	gold quality
artery	UBERON:0001637	97.69	gold quality
adipose tissue	UBERON:0001013	97.68	gold quality
cortex of kidney	UBERON:0001225	97.68	gold quality
adrenal cortex	UBERON:0001235	97.68	gold quality
colonic mucosa	UBERON:0000317	97.66	gold quality
left adrenal gland cortex	UBERON:0035825	97.66	gold quality
renal glomerulus	UBERON:0000074	97.65	gold quality
sigmoid colon	UBERON:0001159	97.65	gold quality
cardiac ventricle	UBERON:0002082	97.65	gold quality
heart left ventricle	UBERON:0002084	97.65	gold quality

Single-cell (SCXA)

Detected in 6 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-HCAD-4	yes	19.68
E-HCAD-10	yes	16.94
E-MTAB-7249	no	37194.32
E-MTAB-6075	no	696.64
E-MTAB-7303	no	438.65
E-ANND-3	no	0.00

HPA expression

RNA tissue specificity: Low tissue specificity

showing 60 of 159 HPA tissues / cells by nTPM:

Tissue / cell	Axis	nTPM	Protein (IHC)
Skeletal muscle	tissue	122.6	low
Pons	tissue	113.9
Thalamus	tissue	109.7
Locus coeruleus	tissue	108.8
Superior colliculi	tissue	108.1
Tongue	tissue	106.9
Caudate putamen	tissue	104.6
Midbrain	tissue	101.5
Frontal cortex	tissue	101.2
Spinal cord	tissue	94.8
Medulla oblongata	tissue	94.4
Hippocampal formation	tissue	94.4
Hypothalamus	tissue	93.0
Substantia nigra	tissue	91.1
Putamen	tissue	90.3
Septum	tissue	89.3
Postcentral gyrus	tissue	88.8
Cerebral cortex	tissue	87.8	medium
Somatosensory cortex	tissue	87.8
Heart muscle	tissue	87.1	high
Periaqueductal gray	tissue	85.7
Motor cortex	tissue	83.6
Occipital cortex	tissue	80.8
Ventral forebrain	tissue	79.5
Kidney	tissue	79.3	high
Retina	tissue	79.2
Parathyroid gland	tissue	78.9	low
Retrosplenial and cingulate cortex	tissue	77.1
Prefrontal cortex	tissue	75.9
Basal ganglia	tissue	75.1	low
Liver	tissue	75.1	medium
Hippocampus	tissue	74.1	medium
Entorhinal cortex	tissue	73.9
Nucleus accumbens	tissue	73.8
Corpus callosum	tissue	73.2
Temporal cortex	tissue	73.2
Retrosplenial cortex	tissue	72.9
Thyroid gland	tissue	72.7	high
Cerebellum	tissue	71.0	medium
Amygdala	tissue	70.7
Pancreas	tissue	67.9	medium
Adrenal gland	tissue	66.2	high
Adipose tissue	tissue	66.0	medium
Urinary bladder	tissue	65.8	medium
Choroid plexus	tissue	64.7
Insular cortex	tissue	64.4
Smooth muscle	tissue	64.3	high
Thymus	tissue	61.9
Placenta	tissue	60.8	high
Olfactory bulb	tissue	59.5
Esophagus	tissue	57.2	medium
Tonsil	tissue	53.6	high
Pituitary gland	tissue	53.5
Colon	tissue	52.0	high
Appendix	tissue	51.7	medium
Lymph node	tissue	51.3	high
Rectum	tissue	49.7	medium
Endometrium 1	tissue	48.9	medium
Bone marrow	tissue	48.9	medium
Cervix	tissue	48.7	medium

Brain sub-regions (HPA Brain Atlas): detected in 483; highest nTPM — Arcuate nucleus (115.1), Lateral hypothalamic area (111.0), Ventromedial nucleus (100.7), Supraoptic nucleus (95.2), Ventral spinal cord (94.8).

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

Targeting STRAP by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific).

showing 30 of 54 miRNAs:

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-223-3P	99.99	70.14	1140
HSA-MIR-34A-5P	99.99	71.21	1784
HSA-MIR-449A	99.99	71.05	1776
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-34C-5P	99.97	70.45	1577
HSA-MIR-449B-5P	99.97	70.26	1580
HSA-LET-7C-3P	99.95	73.42	2862
HSA-MIR-4778-3P	99.93	70.40	1818
HSA-MIR-10523-5P	99.91	69.22	2038
HSA-MIR-652-5P	99.91	67.49	505
HSA-MIR-10527-5P	99.91	72.28	3754
HSA-MIR-6499-3P	99.90	66.38	1212
HSA-MIR-6715A-3P	99.83	68.05	1473
HSA-MIR-4659A-3P	99.80	72.62	4248
HSA-MIR-4659B-3P	99.80	72.62	4248
HSA-MIR-4645-3P	99.76	69.33	993
HSA-MIR-3059-5P	99.70	69.93	2491
HSA-MIR-6892-3P	99.68	66.40	1178
HSA-MIR-3177-5P	99.65	70.38	1174
HSA-MIR-1303	99.65	69.77	1662
HSA-MIR-549A-3P	99.54	68.17	825
HSA-MIR-12123	99.52	71.79	2990
HSA-MIR-642A-5P	99.51	65.10	1152
HSA-MIR-942-5P	99.41	68.40	1977
HSA-MIR-6719-3P	99.29	67.78	1387
HSA-MIR-664A-3P	99.22	71.08	2696
HSA-MIR-122B-3P	99.21	68.90	1333
HSA-MIR-21-3P	99.21	68.95	1312

Functional genomics

gnomAD constraint: pLI 0.844, LOEUF 0.567, missense Z 2.46 — population loss-of-function intolerance (pLI ≥ 0.9 = LoF-intolerant; lower LOEUF = more constrained). gnomAD

Regional missense constraint (gnomAD)

ENST00000419869 splits into 5 regions with different missense tolerance; 1 is missense-constrained (≈18% of the expected missense variation). Missense variants in a constrained region (low observed/expected) are more likely to be pathogenic. gnomAD

Region (GRCh38)	Missense obs / exp	o/e	Constrained
12:15,882,708–15,882,798	31 / 39.2	0.79	no
12:15,882,799–15,889,954	32 / 76.1	0.42	no
12:15,889,955–15,897,968	152 / 183.0	0.83	no
12:15,897,969–15,900,950	26 / 76.7	0.34	yes (p 7.1e-09)
12:15,900,951–15,902,975	52 / 45.0	1.16	no

Literature-anchored findings (GeneRIF, showing 27)

• unrip functions in the pathway of small nuclear ribonucleoproteins biogenesis (PMID:15848170)
• biochemical evidence for the existence of another, yet atypical, SMN complex component, termed unr-interacting protein (unrip) (PMID:16159890)
• STRAP acts as an intermediate signaling molecule linking between the phosphatidylinositol 3-kinase/PDK1 and the TGF-beta signaling pathways (PMID:16251192)
• Gemin8 has an essential role in the proper structural organization of the SMN complex and the involvement of the heteromeric subunit containing Gemin6, Gemin7, Gemin8, and Unrip in the recruitment of Sm proteins to the snRNP assembly pathway (PMID:17023415)
• the direct interaction of NM23-H1 and STRAP is important for the regulation of TGF-beta-dependent biological activity as well as NM23-H1 activity (PMID:17314099)
• These results indicate that upregulation of STRAP might play a role in tumor development as an early event for colorectal cancers. (PMID:17316412)
• an interplay between PPM1G and unrip determine compartment-specific phosphorylation patterns, localization, and function of the SMN complex (PMID:17984321)
• Strap regulation reflects the coordinated interplay between different DNA damage-activated protein kinases, ATM and Chk2 (Checkpoint kinase 2), where phosphorylation by each kinase provides a distinct functional consequence on the activity of Strap. (PMID:18833288)
• STRAP can act as a negative regulator of ASK1. (PMID:19880523)
• gonucleotide pulldown identified STRAP (serine/threonine kinase receptor-associated protein) as a further protein recruited to the MMP28 promoter and acting functionally with Sp1. (PMID:20144149)
• B-MYB acts as a positive regulator of STRAP (PMID:21148321)
• STRAP protein decreases ubiquitination of intracellular domain of Notch3 and may help to stabilize it. (PMID:21502811)
• both of the TPR and OB-fold domains localize to the chromatin of p53 target genes and exhibit intrinsic regulatory activity necessary for the Strap-dependent p53 response (PMID:22362889)
• Coexpression of MAWBP and MAWD inhibited epithelial mesenchymal transformation, and EMT-aided malignant cell progression was suppressed in gastric cancer. (PMID:23687415)
• Data indicate serine-threonine kinase receptor-associated protein (STRAP) as a novel regulator of the coordinated translation of collagen alpha1(I) and alpha2(I) mRNAs. (PMID:23918805)
• Strap augments the apoptotic effects of mitochondrial p53. (PMID:25168243)
• STRAP inhibits the transactivation function of Sp1 either by directly blocking its DNA binding domain or destabilizing Sp1 protein through ubiquitin-proteasome pathway in cell cycle G1 phase. (PMID:25483064)
• MAWD and MAWBP were downregulated and associated with the differentiation grade in GC tissues. (PMID:26373288)
• these results suggest that STRAP increases invasion and metastasis of colorectal cancer partly through inhibiting ubiquitin-dependent degradation of beta-catenin and promoting Wnt/beta-catenin signaling (PMID:26910283)
• Findings illuminated a novel STRAP-NOTCH1-HES1 molecular axis as a CSC regulator in colorectal cancer. (PMID:28827371)
• STRAP plays an inhibitory role in hepatocellular carcinoma via regulating PTEN expression.STRAP is down-regulated in the hepatocellular carcinoma tissues. (PMID:29283497)
• Study found that STRAP and MELK were overexpressed and highly phosphorylated in samples form colorectal adenocarcinoma patients and their expression were significantly correlated with tumor stages. (PMID:29783958)
• Strap associates with Csde1 and affects expression of select Csde1-bound transcripts (PMID:30138317)
• Results show that the increased STRAP protein levels observed in HCC provide growth advantage among others by enhancing Wnt/beta-catenin signaling. (PMID:30257989)
• SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability. (PMID:32527012)
• Serine-threonine Kinase Receptor-Associated Protein is a Critical Mediator of APC Mutation-Induced Intestinal Tumorigenesis Through a Feed-Forward Mechanism. (PMID:34520730)
• Serine-Threonine Kinase Receptor Associate Protein (STRAP) confers an aggressive phenotype in neuroblastoma via regulation of Focal Adhesion Kinase (FAK). (PMID:35272839)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	strap	ENSDARG00000007405
mus_musculus	Strap	ENSMUSG00000030224
rattus_norvegicus	Strap	ENSRNOG00000007134
drosophila_melanogaster	wmd	FBGN0034876

Mouse-model phenotypes

Observed phenotypes of the mouse ortholog (MGI:1329037) from MGI (Alliance of Genome Resources) — knockout/mutant phenotypes, ranked by annotation count. 7 distinct phenotype terms.

Mouse phenotype (MP)	Records
abnormal angiogenesis	1
abnormal heart development	1
abnormal somite development	1
abnormal embryo turning	1
abnormal neural tube closure	1
decreased incidence of tumors by chemical induction	1
embryonic lethality during organogenesis, complete penetrance	1

Additional cross-species homologs

Sequence homologs in species beyond Ensembl Compara’s model organisms, by Diamond local protein alignment (% sequence identity; best hit per species). Lower-identity rows are more distant homologs. The matched accessions’ gene symbols aren’t in biobtree yet, so each row shows the UniProt accession.

Organism	UniProt	Identity	Method
Bos taurus	Q5E959	99.1%	Diamond
Aspergillus fischeri NRRL 181	A1DMI8	96.5%	Diamond
Pyricularia oryzae 70-15	G4MQX3	94.0%	Diamond
Neurospora crassa OR74A	Q01369	94.0%	Diamond
Aspergillus clavatus NRRL 1	A1CTE6	91.8%	Diamond
Gallus gallus	Q5ZL33	89.4%	Diamond
Coccidioides immitis RS	Q1DRB6	78.1%	Diamond
Oryza sativa Japonica Group	Q9AYE4	77.0%	Diamond
Schizosaccharomyces pombe 972h-	O74184	65.2%	Diamond
Arabidopsis thaliana	Q9SXY1	63.6%	Diamond
Yarrowia lipolytica CLIB122	Q6C4I9	63.3%	Diamond
Saccharomyces cerevisiae S288C	P25635	55.7%	Diamond
Dictyostelium discoideum	Q54LT8	48.5%	Diamond
Caenorhabditis elegans	G5EFW7	39.7%	Diamond

Genomic neighbors (locus context)

Genes flanking this one on the chromosome (NCBI/Entrez gene neighborhood). Positional context only — some neighbors share regulatory elements (bidirectional promoters, antisense/overlapping transcripts), but chromosomal adjacency is not itself a functional relationship (see Interactions for that).

Symbol	Type	Entrez
MGST1	protein-coding	4257
DERA	protein-coding	51071
SLC15A5	protein-coding	729025
EPS8	protein-coding	2059
PTPRO	protein-coding	5800

Protein

Protein identifiers

Serine-threonine kinase receptor-associated protein — Q9Y3F4 (reviewed: Q9Y3F4)

Alternative names: MAP activator with WD repeats, UNR-interacting protein, WD-40 repeat protein PT-WD

All UniProt accessions (3): Q9Y3F4, F5H1G1, H0YH33

UniProt curated annotations — full annotation on UniProt →

Function. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.

Subunit / interactions. Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Associates with the SMN complex in the cytoplasm but not in the nucleus. Interacts with GEMIN6; the interaction is direct. Interacts with GEMIN7; the interaction is direct. Interacts with CSDE1/UNR and MAWBP. Interacts with PDPK1. Interacts with TRIM48.

Subcellular location. Cytoplasm. Nucleus.

Similarity. Belongs to the WD repeat STRAP family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9Y3F4-1	1	yes
Q9Y3F4-2	2

RefSeq proteins (1): NP_009109* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001680	WD40_rpt	Repeat
IPR015943	WD40/YVTN_repeat-like_dom_sf	Homologous_superfamily
IPR020472	WD40_PAC1	Repeat
IPR036322	WD40_repeat_dom_sf	Homologous_superfamily
IPR056551	Beta-prop_NOL10_N	Domain

Pfam: PF00400, PF23098

UniProt features (15 total): repeat 7, modified residue 3, sequence conflict 3, chain 1, splice variant 1

Structure

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9Y3F4-F1	89.75	0.84

Human Protein Atlas

Protein class: Predicted intracellular proteins, Plasma proteins, Cancer-related genes, Candidate cancer biomarkers, Essential proteins

Protein evidence: Evidence at protein level

Subcellular location (HPA, imaging): cytosol; cell junctions

Antibody reliability (HPA): IH approved · IF supported

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3)

Type	Positions
Phosphoserine	338, 312, 335

Function

Pathways and Gene Ontology

Reactome pathways

1 curated (TAS) of 14; 0 disease pathways. Curated-first; evidence = TAS (manually curated) vs IEA (electronic inference).

14 pathways:

ID	Pathway	Evidence
R-HSA-9764790	Positive Regulation of CDH1 Gene Transcription	curated
R-HSA-418990	Adherens junctions interactions	—
R-HSA-446728	Cell junction organization	—
R-HSA-1500931	Cell-Cell communication	—
R-HSA-421270	Cell-cell junction organization	—
R-HSA-2173788	Downregulation of TGF-beta receptor signaling	electronic
R-HSA-9764265	Regulation of CDH1 Expression and Function	—
R-HSA-9764560	Regulation of CDH1 Gene Transcription	—
R-HSA-9764274	Regulation of Expression and Function of Type I Classical Cadherins	—
R-HSA-9759476	Regulation of Homotypic Cell-Cell Adhesion	—
R-HSA-162582	Signal Transduction	—
R-HSA-170834	Signaling by TGF-beta Receptor Complex	—
R-HSA-9006936	Signaling by TGFB family members	—
R-HSA-2173789	TGF-beta receptor signaling activates SMADs	—

MSigDB gene sets: 196 (showing top): GOBP_DIGESTION, GOBP_REGULATION_OF_CELLULAR_RESPONSE_TO_GROWTH_FACTOR_STIMULUS, REACTOME_SIGNALING_BY_TGF_BETA_RECEPTOR_COMPLEX, PAL_PRMT5_TARGETS_UP, MODULE_151, GOBP_ALTERNATIVE_MRNA_SPLICING_VIA_SPLICEOSOME, MORF_UBE2I, GRAESSMANN_APOPTOSIS_BY_SERUM_DEPRIVATION_UP, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, DARWICHE_SKIN_TUMOR_PROMOTER_DN, DARWICHE_PAPILLOMA_RISK_LOW_DN, DARWICHE_PAPILLOMA_RISK_HIGH_DN, DARWICHE_SQUAMOUS_CELL_CARCINOMA_DN, MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP, REACTOME_ADHERENS_JUNCTIONS_INTERACTIONS

GO Biological Process (9): alternative mRNA splicing, via spliceosome (GO:0000380), spliceosomal snRNP assembly (GO:0000387), cell differentiation (GO:0030154), maintenance of gastrointestinal epithelium (GO:0030277), negative regulation of transforming growth factor beta receptor signaling pathway (GO:0030512), mRNA splicing, via spliceosome (GO:0000398), mRNA processing (GO:0006397), RNA splicing (GO:0008380), protein-RNA complex assembly (GO:0022618)

GO Molecular Function (5): RNA binding (GO:0003723), mRNA binding (GO:0003729), signaling receptor binding (GO:0005102), U2 snRNP binding (GO:1990447), protein binding (GO:0005515)

GO Cellular Component (5): nucleus (GO:0005634), cytoplasm (GO:0005737), cytosol (GO:0005829), SMN complex (GO:0032797), SMN-Sm protein complex (GO:0034719)

Reactome top-level categories

12 top-level categories:

Category	Pathways
TGF-beta receptor signaling activates SMADs	1
Regulation of CDH1 Gene Transcription	1
Signaling by TGFB family members	1
Signaling by TGF-beta Receptor Complex	1
Cell-cell junction organization	1
Cell junction organization	1
Cell-Cell communication	1
Signal Transduction	1
Adherens junctions interactions	1
Regulation of Expression and Function of Type I Classical Cadherins	1
Regulation of Homotypic Cell-Cell Adhesion	1
Regulation of CDH1 Expression and Function	1

GO top-level categories

27 GO namespaces:

Category	Terms
mRNA splicing, via spliceosome	2
RNA processing	2
cellular anatomical structure	2
cytoplasm	2
Sm-like protein family complex	2
protein-RNA complex assembly	1
cellular developmental process	1
epithelial structure maintenance	1
digestive system process	1
transforming growth factor beta receptor signaling pathway	1
regulation of transforming growth factor beta receptor signaling pathway	1
negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway	1
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	1
mRNA processing	1
mRNA metabolic process	1
ribonucleoprotein complex biogenesis	1
protein-containing complex assembly	1
protein-RNA complex organization	1
nucleic acid binding	1
RNA binding	1
protein binding	1
snRNP binding	1
binding	1
intracellular membrane-bounded organelle	1
intracellular anatomical structure	1
SMN-Sm protein complex	1
SMN complex	1

Experimentally-supported GO annotations

GO terms backed by direct experimental evidence (ECO experimental codes) — 8 of 19 annotations. The rest are phylogenetic / computational / electronic (IEA).

8 experimentally-supported GO terms:

GO term	NS	Evidence
maintenance of gastrointestinal epithelium	BP	mutant phenotype
negative regulation of transforming growth factor beta receptor signaling pathway	BP	mutant phenotype
spliceosomal snRNP assembly	BP	direct assay
SMN complex	CC	direct assay
SMN-Sm protein complex	CC	direct assay
cytoplasm	CC	direct assay
cytosol	CC	direct assay
RNA binding	MF	high-throughput assay

Protein interactions and networks

STRING

showing 15 of 1,648 interactions by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
STRAP	PBLD	P30039	948
STRAP	GEMIN2	O14893	908
STRAP	GEMIN7	Q9H840	902
STRAP	CSDE1	O75534	846
STRAP	LSM2	Q9Y333	815
STRAP	SMN1	Q16637	754
STRAP	GEMIN8	Q9NWZ8	741
STRAP	DDX20	Q9UHI6	712
STRAP	GEMIN5	Q8TEQ6	673
STRAP	GEMIN6	Q8WXD5	651
STRAP	GEMIN4	P57678	606
STRAP	LARP6	Q9BRS8	598
STRAP	SNRPD1	P13641	551
STRAP	CLNS1A	P54105	470
STRAP	LSM10	Q969L4	455

IntAct

Human interaction partners of STRAP in IntAct, one row per partner (best MIscore; records = supporting IntAct interaction records).

29 partners by confidence:

Partner	Interaction type	MIscore	Records
GEMIN6	direct interaction	0.760	2
GEMIN7	direct interaction	0.760	2
NME1	physical association	0.670	9
HTT	physical association	0.670	3
SNRPB	direct interaction	0.590	1
SNRPD2	direct interaction	0.590	1
SZRD1	physical association	0.560	3
CDK2AP1	association	0.530	1
CHEK2	phosphorylation reaction	0.440	1
SNRPD3	direct interaction	0.440	1
SNRPE	direct interaction	0.440	1
SGTA	physical association	0.400	1
TGFBR1	physical association	0.400	1
GEMIN2	association	0.350	2
RAPH1	association	0.350	1
ACTR6	physical association	0.000	1
EIF1B	physical association	0.000	1
GH1	physical association	0.000	1
GSK3B	physical association	0.000	1
HLA-B	physical association	0.000	1
HUS1	physical association	0.000	1
IKBKE	physical association	0.000	1
MTRES1	physical association	0.000	1
NME2	physical association	0.000	1
PRKAB1	physical association	0.000	1
PTP4A3	physical association	0.000	1
RNPS1	physical association	0.000	1
TNIK	physical association	0.000	1
TRAF6	physical association	0.000	1

BioGRID (428 interactions; top human partners by supporting records): CSDE1 (Affinity Capture-MS; 14 records), GEMIN7 (Affinity Capture-MS; 7 records), SMN1 (Affinity Capture-Western; 7 records), DDX20 (Affinity Capture-MS; 6 records), GEMIN2 (Affinity Capture-MS; 5 records), GEMIN6 (Affinity Capture-MS; 5 records), GEMIN8 (Affinity Capture-MS; 5 records), SZRD1 (Affinity Capture-MS; 5 records), GEMIN4 (Affinity Capture-MS; 4 records), GEMIN5 (Affinity Capture-MS; 4 records), SMAD3 (Phenotypic Suppression; 4 records), SMAD7 (Phenotypic Enhancement; 4 records), EGFR (Negative Genetic; 3 records), EP300 (Affinity Capture-MS; 3 records), GSK3B (Affinity Capture-MS; 3 records)

SIGNOR signaling

1 signaling interactions:

A	Effect	B	Mechanism
SMAD7	up-regulates	STRAP	binding

Protein complexes (CORUM) (5)

Experimentally-characterized complexes this protein is a subunit of (★ = the complex contains a known drug target).

5 complexes by size:

Complex	Subunits	Members
Spliceosome, E complex	129	RBM17, DDX39A, GEMIN2, NCBP2, PRPF31, SART3, SF3A2, SNRPA, SRSF7, DDX39B, DHX15, GEMIN5, SCAF4, SMARCB1, SNRPD3, SYNCRIP, U2AF2, HNRNPC, SF3B2, SMARCC2, SFSWAP, USP39, GEMIN6, HNRNPK, RBM42, SF3B1, SF3B4, SNRPG, SRSF6, BCAS2, SMN1, CPSF7, SMARCE1, SRSF8, ARID1A, LSM4, SNRPC, CHD8, FUS, SNRNP70, SRSF11, NUDT21, PBRM1, SNRPB2, SNRPD1, YBX1, SF1, SNRPD2, SRSF2, SRSF5, PPIH, SRSF3, DDX20, SART1, SRSF1, SRSF4, PRPF19, RBM39, RBMXL2, SREK1, PRPF3, SMARCA2, U2AF1, SF3B6, SMARCA1, CPSF1, DDX23, DHX9, RUVBL1, SNRPF, SRRM2, ALYREF, HNRNPA0, HNRNPH1, PCBP2, RBM10, SF3B3, STRAP, HNRNPR, RBM25, ACTBL2, CHERP, SNRPA1, ACTL6A, DDX46, HNRNPL, RBM7, CPSF6, ARID2, DNAJC8, EFTUD2, KHSRP, RUVBL2, SMARCA4, PCBP1, SNRNP40, SNRPE, PRPF4, PRPF8, SF3A1, SF3A3, SNRNP200, ZCCHC8, DDX5, LUC7L3, SMARCC1, HNRNPA2B1, HNRNPD, HTATSF1, LUC7L2, PHF5A, PRPF6, SMARCD1, SNRPB, GEMIN8, U2SURP, NCBP1, GEMIN4, HNRNPU, MTREX, ARID1B, CHD4, HNRNPM, PRPF40A, HNRNPF, SRRT, DDX17, HNRNPA1, PUF60
SMN complex (16 subunits)	16	SNRPD3, SNRPG, GEMIN4, GEMIN5, SNRPB, SNRPF, DDX20, GEMIN7, SMN1, STRAP, SNRPD1, SNRPD2, SNRPE, GEMIN2, GEMIN6, GEMIN8
SMN complex	10	DDX20, GEMIN5, GEMIN6, GEMIN7, SNRPA, SNRPB, STRAP, GEMIN2, GEMIN4, SMN1
★ AXIN1-GSK3B-STRAP complex	3	AXIN1, GSK3B, STRAP
GEMIN6-GEMIN7-STRAP complex, SMN-independent intermediate	3	GEMIN6, GEMIN7, STRAP

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 29 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
SARS-CoV-2 modulates host translation machinery	7	65.3×	1e-09
snRNP Assembly	7	61.7×	1e-09
mRNA Splicing	5	22.9×	6e-05
Processing of Capped Intron-Containing Pre-mRNA	5	17.1×	2e-04
mRNA Splicing - Major Pathway	5	11.4×	9e-04
Dengue Virus-Host Interactions	5	9.5×	2e-03
Metabolism of RNA	5	8.7×	2e-03

GO biological processes:

GO term	Partners	Fold	FDR
spliceosomal snRNP assembly	7	145.3×	9e-12
mRNA splicing, via spliceosome	7	22.9×	2e-06
RNA splicing	5	15.8×	8e-04

Disease & clinical

Genetic variants

Variant evidence for STRAP: 45 ClinVar records (0 pathogenic / likely pathogenic).

ClinVar

45 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	23
Likely benign	0
Benign	0

AlphaMissense

1,272 missense substitutions predicted likely pathogenic (of 2,300 scored), across 258 residues. Top 20 hotspot residues, ranked by the number of likely-pathogenic substitutions (of those reachable by a single-nucleotide change), then by mean score:

Residue	Likely-pathogenic substitutions	Mean am_pathogenicity	Max am_pathogenicity
M42	9	0.980	0.999
H13	8	1.000	1.000
D80	8	1.000	1.000
D286	8	1.000	1.000
D38	8	0.999	1.000
D50	8	0.999	1.000
H58	8	0.999	1.000
H264	8	0.999	1.000
H269	8	0.997	1.000
H101	8	0.996	0.999
F265	8	0.994	1.000
F55	8	0.993	1.000
D244	8	0.993	1.000
H99	8	0.991	0.998
D276	8	0.990	0.999
D163	8	0.986	0.999
S274	8	0.986	1.000
F22	8	0.984	1.000
H142	8	0.982	0.997
S34	8	0.978	1.000

HPA cancer prognostics

RNA cancer specificity: Low cancer specificity

Human Protein Atlas survival analysis — favorable = higher expression predicts better survival.

11 prognostic cancer types:

Cancer	Prognostic	p-value
Kidney Renal Clear Cell Carcinoma (TCGA)	favorable	1.56e-06
Lung Adenocarcinoma (TCGA)	unfavorable	5.72e-06
Kidney Renal Clear Cell Carcinoma (TCGA)	favorable	5.89e-06
Liver Hepatocellular Carcinoma (validation)	unfavorable	1.41e-05
Kidney Renal Clear Cell Carcinoma (TCGA)	favorable	3.28e-05
Liver Hepatocellular Carcinoma (TCGA)	unfavorable	3.55e-05
Ovary Serous Cystadenocarcinoma (TCGA)	unfavorable	0.000107
Ovary Serous Cystadenocarcinoma (validation)	unfavorable	0.00011
Pancreatic Adenocarcinoma (validation)	unfavorable	0.000159
Kidney Chromophobe (TCGA)	unfavorable	0.000526
Bladder Urothelial Carcinoma (TCGA)	unfavorable	0.000832

Disease associations

OMIM: gene MIM:605986 | disease phenotypes:

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067330 (SINGLE PROTEIN)

PharmGKB: 1 entry (CPIC guideline: no)

Binding affinities (BindingDB)

1 measured of 1 human assays (2 total across all organisms); most potent 1 below. Values come from heterogeneous assays and are not directly comparable.

Ligand	Measure	Value
CHEMBL5653589	KD	1440 nM

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.75	Kd	17.7	nM	CHEMBL5653589
7.75	ED50	17.7	nM	CHEMBL5653589
6.64	Kd	231.2	nM	CHEMBL3752910
6.64	ED50	231.2	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149517: Binding affinity to human STRAP incubated for 45 mins by Kinobead based pull down assay	kd	0.0177	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149517: Binding affinity to human STRAP incubated for 45 mins by Kinobead based pull down assay	kd	0.2312	uM

CTD chemical–gene interactions

37 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects expression, decreases expression, decreases methylation	4
Tobacco Smoke Pollution	affects expression, increases expression	2
aristolochic acid I	increases expression	1
FR900359	increases phosphorylation	1
bisphenol F	increases expression	1
moringin	increases expression	1
TAK-243	increases sumoylation	1
deoxynivalenol	increases expression	1
trichostatin A	increases expression	1
sodium arsenite	decreases expression	1
tetrabromobisphenol A	decreases expression	1
2-bromopalmitate	increases palmitoylation, decreases reaction, increases abundance	1
perfluorooctanoic acid	decreases expression	1
ochratoxin A	increases expression	1
chloropicrin	increases expression	1
bisphenol B	increases expression	1
bisphenol S	increases expression	1
bisphenol AF	increases expression	1
Arsenic Trioxide	increases expression	1
Vorinostat	decreases expression	1
Acetaminophen	decreases expression	1
Air Pollutants, Occupational	affects expression	1
Benztropine	decreases expression	1
Cadmium	increases abundance, increases palmitoylation, decreases reaction	1
Cannabidiol	increases expression	1
Clozapine	decreases expression	1
Doxorubicin	affects expression	1
Enzyme Inhibitors	decreases activity, increases O-linked glycosylation	1
Ivermectin	decreases expression	1
Phthalic Acids	increases methylation	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652559	Binding	Binding affinity to human STRAP incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — *ChemMedChem*

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3II	Abcam HEK293T STRAP KO	Transformed cell line	Female

Clinical trials (associated diseases)

Via MONDO — disease-level, not drug-specific.

Predicted chemistry — Sugi Predict

The predicted patent-compound landscape for STRAP across ~30M SureChEMBL compounds — explore on Sugi Predict.