TADA1

Summary

TADA1 (transcriptional adaptor 1, HGNC:30631) is a protein-coding gene on chromosome 1q24.1, encoding Transcriptional adapter 1 (Q96BN2). Probably involved in transcriptional regulation. It is a selective cancer dependency (DepMap: 26.7% of cell lines).

TADA1L is a protein subunit of the human STAGA complex (SPT3; (MIM 602947)/TAF9 (MIM 600822)/GCN5 (MIM 602301) acetyltransferase complex), which is a chromatin-modifying multiprotein complex (Martinez et al., 2001 [PubMed 11564863]).

Source: NCBI Gene 117143 — RefSeq curated summary.

At a glance

• GWAS associations: 9
• Clinical variants (ClinVar): 61 total
• Cancer dependency (DepMap): dependent in 26.7% of screened cell lines
• MANE Select transcript: NM_053053

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30631
Approved symbol	TADA1
Name	transcriptional adaptor 1
Location	1q24.1
Locus type	gene with protein product
Status	Approved
Aliases	STAF42, ADA1, hADA1, HFI1
Ensembl gene	ENSG00000152382
Ensembl biotype	protein_coding
OMIM	612763
Entrez	117143

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 5 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000367874, ENST00000467021, ENST00000910858, ENST00000910859, ENST00000910860, ENST00000972177

RefSeq mRNA: 1 — MANE Select: NM_053053 NM_053053

CCDS: CCDS1255

Canonical transcript exons

ENST00000367874 — 8 exons

Exon	Start	End
ENSE00001004938	166862203	166862412
ENSE00001004941	166869445	166869510
ENSE00001004942	166869763	166869854
ENSE00001067415	166863824	166863921
ENSE00001445824	166876160	166876264
ENSE00003509196	166856510	166857719
ENSE00003534704	166858119	166858281
ENSE00003692736	166860186	166860337

Expression profiles

Bgee: expression breadth ubiquitous, 247 present calls, max score 97.51.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 11.9040 / max 172.4219, expressed in 1733 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
15747	10.7969	1695
15748	1.1071	684

Top tissues by expression

251 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	97.51	gold quality
oocyte	CL:0000023	94.96	gold quality
cortical plate	UBERON:0005343	89.49	gold quality
ganglionic eminence	UBERON:0004023	88.56	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	88.29	gold quality
Brodmann (1909) area 46	UBERON:0006483	88.19	gold quality
ileal mucosa	UBERON:0000331	87.32	gold quality
right lobe of liver	UBERON:0001114	87.08	gold quality
ventricular zone	UBERON:0003053	86.84	gold quality
prefrontal cortex	UBERON:0000451	86.64	gold quality
islet of Langerhans	UBERON:0000006	86.46	gold quality
tibialis anterior	UBERON:0001385	85.58	gold quality
cartilage tissue	UBERON:0002418	85.35	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	85.16	gold quality
liver	UBERON:0002107	85.11	gold quality
adult organism	UBERON:0007023	85.01	gold quality
pigmented layer of retina	UBERON:0001782	84.96	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	84.88	gold quality
Brodmann (1909) area 9	UBERON:0013540	84.74	gold quality
biceps brachii	UBERON:0001507	83.77	gold quality
frontal cortex	UBERON:0001870	83.77	gold quality
neocortex	UBERON:0001950	83.54	gold quality
deltoid	UBERON:0001476	82.84	gold quality
cerebral cortex	UBERON:0000956	82.79	gold quality
placenta	UBERON:0001987	82.73	gold quality
anterior cingulate cortex	UBERON:0009835	82.71	gold quality
cerebellar hemisphere	UBERON:0002245	82.56	gold quality
cerebellar cortex	UBERON:0002129	82.52	gold quality
rectum	UBERON:0001052	82.35	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	82.19	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-MTAB-3929	yes	148.27
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

79 targeting TADA1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6798-5P	100.00	65.77	699
HSA-MIR-4262	100.00	73.26	3931
HSA-MIR-4673	100.00	66.64	1490
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-188-3P	100.00	68.76	1240
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-181A-5P	99.99	72.96	2995
HSA-MIR-181B-5P	99.99	72.97	2996
HSA-MIR-181C-5P	99.99	72.95	2996
HSA-MIR-181D-5P	99.99	73.04	2997
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-4645-5P	99.98	65.81	1284
HSA-MIR-548AT-5P	99.96	70.83	2666
HSA-MIR-23A-3P	99.95	74.24	3163
HSA-MIR-23B-3P	99.95	74.24	3163
HSA-MIR-23C	99.95	73.92	3192
HSA-MIR-141-3P	99.94	72.79	2421
HSA-MIR-200A-3P	99.94	72.68	2420
HSA-MIR-4525	99.94	64.38	675
HSA-MIR-5010-5P	99.94	64.11	705
HSA-MIR-8063	99.91	69.76	3146
HSA-MIR-548E-5P	99.89	72.73	4486
HSA-MIR-4496	99.88	68.89	2236
HSA-MIR-30A-3P	99.87	69.74	2928
HSA-MIR-30D-3P	99.87	69.92	2917
HSA-MIR-30E-3P	99.87	69.68	2942
HSA-MIR-629-3P	99.85	67.99	1875
HSA-MIR-4698	99.84	71.41	4303
HSA-MIR-2052	99.79	69.37	2031

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 26.7% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 1)

• Differences between mother and fetus in ADA(1) phenotype may influence the ratio BW/PW in healthy women and reproductive success in RSA women. (PMID:22287124)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	TADA1	ENSDARG00000100031
mus_musculus	Tada1	ENSMUSG00000026563
rattus_norvegicus	Tada1	ENSRNOG00000003816
drosophila_melanogaster	Ada1-1	FBGN0051865
drosophila_melanogaster	Ada1-2	FBGN0051866

Protein

Protein identifiers

Transcriptional adapter 1 — Q96BN2 (reviewed: Q96BN2)

Alternative names: SPT3-associated factor 42, Transcriptional adapter 1-like protein

All UniProt accessions (1): Q96BN2

UniProt curated annotations — full annotation on UniProt →

Function. Probably involved in transcriptional regulation.

Subunit / interactions. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9.

Subcellular location. Nucleus.

Similarity. Belongs to the TADA1 family.

RefSeq proteins (1): NP_444281* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR024738	Hfi1/Tada1	Family

Pfam: PF12767

UniProt features (19 total): helix 8, strand 3, turn 2, compositionally biased region 2, chain 1, region of interest 1, sequence variant 1, sequence conflict 1

Structure

Experimental structures (PDB)

4 structures.

PDB	Method	Resolution (Å)
9RDK	ELECTRON MICROSCOPY	2.41
7KTR	ELECTRON MICROSCOPY	2.93
8H7G	ELECTRON MICROSCOPY	3.7
7KTS	ELECTRON MICROSCOPY	19.09

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96BN2-F1	77.95	0.38

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-3214847	HATs acetylate histones
R-HSA-3247509	Chromatin modifying enzymes
R-HSA-4839726	Chromatin organization

MSigDB gene sets: 141 (showing top): NAGY_STAGA_COMPONENTS_HUMAN, GOBP_REGULATION_OF_DNA_REPAIR, TTGGGAG_MIR150, PATIL_LIVER_CANCER, MODULE_239, USF_01, GOBP_REGULATION_OF_RESPONSE_TO_STRESS, GOBP_RNA_SPLICING, GOBP_DNA_DAMAGE_RESPONSE, GOBP_REGULATION_OF_CELLULAR_RESPONSE_TO_STRESS, GOBP_REGULATION_OF_RNA_SPLICING, GOCC_SAGA_COMPLEX, GOCC_TRANSFERASE_COMPLEX, GOCC_SAGA_TYPE_COMPLEX, USF2_Q6

GO Biological Process (4): regulation of DNA repair (GO:0006282), regulation of transcription by RNA polymerase II (GO:0006357), regulation of RNA splicing (GO:0043484), positive regulation of DNA-templated transcription (GO:0045893)

GO Molecular Function (1): transcription coactivator activity (GO:0003713)

GO Cellular Component (6): SAGA complex (GO:0000124), nucleus (GO:0005634), nucleoplasm (GO:0005654), cytosol (GO:0005829), focal adhesion (GO:0005925), SAGA-type complex (GO:0070461)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Chromatin modifying enzymes	1
Chromatin organization	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of DNA-templated transcription	2
cellular anatomical structure	2
DNA repair	1
regulation of DNA metabolic process	1
regulation of cellular response to stress	1
transcription by RNA polymerase II	1
RNA splicing	1
regulation of gene expression	1
regulation of primary metabolic process	1
DNA-templated transcription	1
positive regulation of RNA biosynthetic process	1
transcription coregulator activity	1
positive regulation of DNA-templated transcription	1
SAGA-type complex	1
DUBm complex	1
peptidase complex	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cytoplasm	1
cell-substrate junction	1
histone acetyltransferase complex	1

Protein interactions and networks

STRING

1443 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TADA1	KAT2A	Q92830	848
TADA1	KAT2B	Q92831	835
TADA1	TAF9	Q16594	800
TADA1	ATXN7	O15265	759
TADA1	SGF29	Q96ES7	732
TADA1	TAF12	Q16514	713
TADA1	SUPT20H	Q8NEM7	667
TADA1	TAF4	O00268	644
TADA1	SUPT7L	O94864	611
TADA1	TADA3	O75528	588
TADA1	ENY2	Q9NPA8	564
TADA1	TRRAP	Q9Y4A5	553
TADA1	TAF6L	Q9Y6J9	550
TADA1	TAF6	P49848	543
TADA1	TAF5	Q15542	491

IntAct

57 interactions, top by confidence:

A	B	Type	Score
SGF29	NDC80	psi-mi:“MI:0914”(association)	0.840
TAF12	TAF4	psi-mi:“MI:0914”(association)	0.760
TRRAP	ATXN7	psi-mi:“MI:0914”(association)	0.740
TADA3	TADA2A	psi-mi:“MI:0914”(association)	0.740
TADA1	TAF5L	psi-mi:“MI:0914”(association)	0.640
ATXN7L3	USP27X	psi-mi:“MI:0914”(association)	0.640
TADA1	TADA3	psi-mi:“MI:0914”(association)	0.640
FOXR2	NME2P1	psi-mi:“MI:0914”(association)	0.530
SUPT20H	ATXN7	psi-mi:“MI:0914”(association)	0.530
TAF6L	SUPT3H	psi-mi:“MI:0914”(association)	0.530
TADA2B	SUPT3H	psi-mi:“MI:0914”(association)	0.530
TADA1	PTCHD1	psi-mi:“MI:0915”(physical association)	0.370
TRRAP	TTI2	psi-mi:“MI:0914”(association)	0.350
SGF29	TRRAP	psi-mi:“MI:0914”(association)	0.350
HNRNPU		psi-mi:“MI:0914”(association)	0.350
MKI67	ARHGAP10	psi-mi:“MI:0914”(association)	0.350
ATXN7L3	USP27X	psi-mi:“MI:0914”(association)	0.350
SGF29	DTNB	psi-mi:“MI:0914”(association)	0.350
PLAC1	OBSL1	psi-mi:“MI:0914”(association)	0.350
TADA3	TADA2A	psi-mi:“MI:0914”(association)	0.350
USP22	CNOT1	psi-mi:“MI:0914”(association)	0.350
ATXN7	SUPT3H	psi-mi:“MI:0914”(association)	0.350
SGF29	USP27X	psi-mi:“MI:0914”(association)	0.350
ENY2	EIF3CL	psi-mi:“MI:0914”(association)	0.350

BioGRID (148): TADA1 (Protein-peptide), TADA1 (Affinity Capture-MS), TADA1 (Affinity Capture-MS), TADA1 (Affinity Capture-MS), TADA1 (Affinity Capture-MS), TAF6L (Affinity Capture-MS), TRRAP (Affinity Capture-MS), TAF9B (Affinity Capture-MS), TAF9 (Affinity Capture-MS), TAF10 (Affinity Capture-MS), HCCS (Affinity Capture-MS), TAF5L (Affinity Capture-MS), TAF12 (Affinity Capture-MS), SUPT7L (Affinity Capture-MS), KAT2B (Affinity Capture-MS)

ESM2 similar proteins: A4IFQ0, A6QL63, A6QR06, A7MAZ4, O75486, P54198, Q08BT5, Q0VA03, Q13769, Q14161, Q15022, Q1LVW0, Q28J24, Q3B7L5, Q5BJQ7, Q5RDB9, Q5ZJA9, Q5ZMS1, Q61666, Q62784, Q66H91, Q68FF6, Q68FX7, Q6DEW4, Q6GMF2, Q6GQW0, Q6ZPY2, Q76JQ2, Q7Z7C8, Q7ZYA2, Q80U70, Q8BIK4, Q8BKT7, Q8C0Q9, Q8NFG4, Q8QZS3, Q96BN2, Q96MD2, Q96PE3, Q99LM9

Diamond homologs: A6QR06, Q5BJQ7, Q5RDB9, Q6DEW4, Q6GMF2, Q6NWA8, Q96BN2, Q99LM9, Q8IP99, Q8IPA1

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
TADA1	“form complex”	“SAGA complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 68 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
HATs acetylate histones	13	24.0×	9e-13
Chromatin organization	11	20.9×	4e-10
Chromatin modifying enzymes	11	18.5×	9e-10
mRNA Polyadenylation	5	10.2×	6e-03
Processing of Capped Intron-Containing Pre-mRNA	5	9.6×	7e-03
Ub-specific processing proteases	7	8.7×	1e-03
mRNA Splicing - Major Pathway	6	7.6×	6e-03

GO biological processes:

GO term	Partners	Fold	FDR
regulation of DNA repair	16	68.0×	2e-23
regulation of RNA splicing	15	50.5×	6e-20
RNA polymerase II preinitiation complex assembly	5	20.9×	2e-04
positive regulation of transcription initiation by RNA polymerase II	5	20.9×	2e-04
transcription by RNA polymerase II	7	7.6×	1e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

61 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	45
Likely benign	1
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

1187 predictions. Top by Δscore:

Variant	Effect	Δscore
1:166858116:TACCT:T	donor_loss	1.0000
1:166858117:A:AC	donor_gain	1.0000
1:166858118:C:CC	donor_gain	1.0000
1:166858118:C:CT	donor_loss	1.0000
1:166858118:CCTG:C	donor_gain	1.0000
1:166858279:GGG:G	acceptor_gain	1.0000
1:166858282:C:CC	acceptor_gain	1.0000
1:166860181:TTTA:T	donor_loss	1.0000
1:166860182:TTAC:T	donor_loss	1.0000
1:166860183:TACC:T	donor_loss	1.0000
1:166860184:A:AT	donor_loss	1.0000
1:166860185:CCTTT:C	donor_gain	1.0000
1:166860211:C:CT	donor_gain	1.0000
1:166860212:T:TT	donor_gain	1.0000
1:166860333:TGATT:T	acceptor_gain	1.0000
1:166860334:GATT:G	acceptor_gain	1.0000
1:166860335:ATT:A	acceptor_gain	1.0000
1:166860336:TT:T	acceptor_gain	1.0000
1:166860336:TTC:T	acceptor_loss	1.0000
1:166860338:C:CA	acceptor_loss	1.0000
1:166860338:C:CC	acceptor_gain	1.0000
1:166860340:G:C	acceptor_gain	1.0000
1:166860340:G:GC	acceptor_gain	1.0000
1:166860345:CA:C	acceptor_gain	1.0000
1:166860346:A:AC	acceptor_gain	1.0000
1:166860346:A:C	acceptor_gain	1.0000
1:166860349:C:CT	acceptor_gain	1.0000
1:166862234:T:TA	donor_gain	1.0000
1:166862408:CTATG:C	acceptor_gain	1.0000
1:166863822:A:AC	donor_gain	1.0000

AlphaMissense

2197 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:166869477:A:G	L67P	1.000
1:166869484:C:G	A65P	1.000
1:166869486:A:G	L64P	1.000
1:166869489:A:G	L63P	1.000
1:166869491:G:C	F62L	1.000
1:166869491:G:T	F62L	1.000
1:166869492:A:G	F62S	1.000
1:166869493:A:G	F62L	1.000
1:166869789:G:T	A47D	1.000
1:166869801:A:G	F43S	1.000
1:166869809:T:A	K40N	1.000
1:166869809:T:G	K40N	1.000
1:166869827:G:C	F34L	1.000
1:166869827:G:T	F34L	1.000
1:166869828:A:G	F34S	1.000
1:166869829:A:G	F34L	1.000
1:166869832:A:G	W33R	1.000
1:166869832:A:T	W33R	1.000
1:166869840:A:G	L30P	1.000
1:166869853:A:G	Y26H	1.000
1:166860330:A:G	L183P	0.999
1:166862267:G:T	A159D	0.999
1:166862268:C:G	A159P	0.999
1:166862404:G:C	F113L	0.999
1:166862404:G:T	F113L	0.999
1:166862405:A:G	F113S	0.999
1:166862406:A:G	F113L	0.999
1:166869469:A:G	C70R	0.999
1:166869480:A:C	I66S	0.999
1:166869480:A:T	I66N	0.999

dbSNP variants (sampled 300 via entrez): RS1000105477 (1:166871590 A>G), RS1000202861 (1:166871903 T>C), RS1000334620 (1:166877012 T>C), RS1000477805 (1:166871837 A>C), RS1000545919 (1:166866174 G>A), RS1000580079 (1:166865845 T>A,G), RS1000654638 (1:166863687 C>G,T), RS1000725813 (1:166865388 T>A,C), RS1000814995 (1:166858678 G>C), RS1000985930 (1:166863466 ACCAGGACC>A), RS1001504450 (1:166876680 C>T), RS1001615595 (1:166878247 G>C), RS1001665912 (1:166857913 TTTTTC>T), RS1001751929 (1:166873176 C>A), RS1001890430 (1:166875790 G>A)

Disease associations

OMIM: gene MIM:612763 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

9 associations (top):

Study	Trait	p-value
GCST010697_48	Cortical surface area (min-P)	3.000000e-13
GCST010698_42	Subcortical volume (min-P)	8.000000e-12
GCST010699_55	Brain morphology (min-P)	2.000000e-40
GCST010700_39	Cortical thickness (MOSTest)	4.000000e-08
GCST010701_100	Cortical surface area (MOSTest)	1.000000e-12
GCST010702_40	Subcortical volume (MOSTest)	2.000000e-10
GCST010703_26	Brain morphology (MOSTest)	5.000000e-09
GCST90002398_500	Neutrophil count	2.000000e-15
GCST90002407_12	White blood cell count	3.000000e-11

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0004346	neuroimaging measurement
EFO:0004840	cortical thickness
EFO:0004833	neutrophil count

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

27 total (human), top 27 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	decreases methylation, affects expression, decreases expression	4
trichostatin A	affects cotreatment, decreases expression, affects expression	3
Cadmium Chloride	decreases expression, increases abundance, increases expression	3
Benzo(a)pyrene	affects methylation, decreases expression	2
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide	decreases expression	2
Aflatoxin B1	decreases methylation, increases methylation	2
aristolochic acid I	decreases expression	1
3-((6-(2-methoxyphenyl)pyrimidin-4-yl)amino)phenyl)methane sulfonamide	decreases expression	1
triphenyl phosphate	affects expression	1
sodium arsenite	decreases expression	1
benzo(e)pyrene	decreases methylation	1
aflatoxin B2	decreases methylation	1
perfluorooctane sulfonic acid	increases expression	1
2-palmitoylglycerol	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
Cadmium	increases abundance, increases expression	1
Dimethyl Sulfoxide	increases expression	1
Formaldehyde	decreases expression	1
Gold	decreases expression	1
Methapyrilene	decreases methylation	1
Quercetin	decreases expression	1
Silver	decreases expression	1
Urethane	decreases expression	1
Cyclosporine	decreases expression	1
Copper Sulfate	decreases expression	1
Lactic Acid	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.