TADA2B

Summary

TADA2B (transcriptional adaptor 2B, HGNC:30781) is a protein-coding gene on chromosome 4p16.1, encoding Transcriptional adapter 2-beta (Q86TJ2). Coactivates PAX5-dependent transcription together with either SMARCA4 or GCN5L2. It is a selective cancer dependency (DepMap: 22.9% of cell lines).

TADA2B functions as a transcriptional adaptor protein that potentiates transcription through coordination of histone acetyltransferase (HAT) activity and by linking activation factors to basal transcriptional machinery (Barlev et al., 2003 [PubMed 12972612]).

Source: NCBI Gene 93624 — RefSeq curated summary.

At a glance

• GWAS associations: 12
• Clinical variants (ClinVar): 32 total
• Cancer dependency (DepMap): dependent in 22.9% of screened cell lines
• MANE Select transcript: NM_152293

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30781
Approved symbol	TADA2B
Name	transcriptional adaptor 2B
Location	4p16.1
Locus type	gene with protein product
Status	Approved
Aliases	MGC21874
Ensembl gene	ENSG00000173011
Ensembl biotype	protein_coding
OMIM	608790
Entrez	93624

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 5 protein_coding

ENST00000310074, ENST00000506692, ENST00000510704, ENST00000512388, ENST00000515646

RefSeq mRNA: 1 — MANE Select: NM_152293 NM_152293

CCDS: CCDS47007

Canonical transcript exons

ENST00000310074 — 2 exons

Exon	Start	End
ENSE00001192497	7054062	7057952
ENSE00001279053	7043372	7043849

Expression profiles

Bgee: expression breadth ubiquitous, 257 present calls, max score 96.23.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 7.6360 / max 639.6168, expressed in 1761 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
46823	7.6360	1761

Top tissues by expression

258 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
tibialis anterior	UBERON:0001385	96.23	gold quality
epithelial cell of pancreas	CL:0000083	96.07	gold quality
vastus lateralis	UBERON:0001379	94.98	gold quality
parotid gland	UBERON:0001831	94.91	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	94.64	gold quality
left ventricle myocardium	UBERON:0006566	94.36	silver quality
endothelial cell	CL:0000115	94.28	gold quality
deltoid	UBERON:0001476	94.22	silver quality
biceps brachii	UBERON:0001507	94.06	gold quality
Brodmann (1909) area 23	UBERON:0013554	93.96	gold quality
quadriceps femoris	UBERON:0001377	93.84	gold quality
kidney epithelium	UBERON:0004819	93.48	silver quality
cardiac muscle of right atrium	UBERON:0003379	93.44	silver quality
skeletal muscle tissue	UBERON:0001134	93.32	gold quality
gastrocnemius	UBERON:0001388	92.69	gold quality
skeletal muscle organ	UBERON:0014892	92.53	gold quality
heart right ventricle	UBERON:0002080	92.34	gold quality
muscle tissue	UBERON:0002385	92.33	gold quality
muscle of leg	UBERON:0001383	92.23	gold quality
caput epididymis	UBERON:0004358	91.73	gold quality
myocardium	UBERON:0002349	91.54	silver quality
cauda epididymis	UBERON:0004360	91.40	gold quality
corpus epididymis	UBERON:0004359	91.35	gold quality
secondary oocyte	CL:0000655	91.33	gold quality
pigmented layer of retina	UBERON:0001782	91.32	gold quality
middle temporal gyrus	UBERON:0002771	90.28	gold quality
trabecular bone tissue	UBERON:0002483	90.24	silver quality
ileal mucosa	UBERON:0000331	90.01	gold quality
mucosa of sigmoid colon	UBERON:0004993	90.01	gold quality
hindlimb stylopod muscle	UBERON:0004252	89.86	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.97

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

125 targeting TADA2B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4262	100.00	73.26	3931
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-200B-3P	100.00	73.31	2693
HSA-MIR-200C-3P	100.00	73.35	2685
HSA-MIR-429	100.00	73.44	2698
HSA-MIR-29A-3P	100.00	73.11	1835
HSA-MIR-29B-3P	100.00	73.18	1833
HSA-MIR-29C-3P	100.00	73.15	1833
HSA-MIR-181D-5P	99.99	73.04	2997
HSA-MIR-181A-5P	99.99	72.96	2995
HSA-MIR-181B-5P	99.99	72.97	2996
HSA-MIR-181C-5P	99.99	72.95	2996
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-3688-3P	99.97	72.02	2834
HSA-MIR-548AN	99.97	70.91	2817
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-4666A-3P	99.96	71.71	3434
HSA-MIR-338-5P	99.92	72.34	2951
HSA-MIR-205-3P	99.92	69.92	3165
HSA-MIR-5680	99.91	69.83	3421
HSA-MIR-3671	99.90	73.04	3897
HSA-MIR-106A-5P	99.90	73.94	2683
HSA-MIR-124-3P	99.89	73.74	3043
HSA-MIR-506-3P	99.89	73.55	3057
HSA-MIR-17-5P	99.89	73.83	2665
HSA-MIR-93-5P	99.88	73.98	2606
HSA-MIR-106B-5P	99.88	74.72	2795

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 22.9% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 1)

• ADA2a and ADA2b, two human homologues of yeast Ada2, each have the ability to form a heterotrimer with ADA3 and GCN5L but that only the ADA2b homologue is found in STAGA. (PMID:18936164)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	tada2b	ENSDARG00000003769
mus_musculus	Tada2b	ENSMUSG00000029196
rattus_norvegicus	Tada2b	ENSRNOG00000027326
drosophila_melanogaster	Ada2b	FBGN0037555
caenorhabditis_elegans		WBGENE00017967

Paralogs (1): TADA2A (ENSG00000276234)

Protein

Protein identifiers

Transcriptional adapter 2-beta — Q86TJ2 (reviewed: Q86TJ2)

Alternative names: ADA2-like protein beta

All UniProt accessions (3): Q86TJ2, D6RC20, D6RJ05

UniProt curated annotations — full annotation on UniProt →

Function. Coactivates PAX5-dependent transcription together with either SMARCA4 or GCN5L2.

Subunit / interactions. Interacts with GCN5L2, SMARCA4, SMARCE1 and PAX5. Component of the TFTC-HAT complex.

Subcellular location. Nucleus.

Isoforms (3)

UniProt ID	Names	Canonical?
Q86TJ2-1	1	yes
Q86TJ2-2	2
Q86TJ2-3	3

RefSeq proteins (1): NP_689506* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000433	Znf_ZZ	Domain
IPR001005	SANT/Myb	Domain
IPR009057	Homeodomain-like_sf	Homologous_superfamily
IPR016827	Ada2/TADA2	Family
IPR017884	SANT_dom	Domain
IPR036388	WH-like_DNA-bd_sf	Homologous_superfamily
IPR041983	ADA2-like_ZZ	Domain
IPR043145	Znf_ZZ_sf	Homologous_superfamily
IPR055141	TADA2A_B-like_dom	Domain
IPR056267	Ada2b_C	Domain

Pfam: PF00249, PF22941, PF24533, PF25299

UniProt features (15 total): binding site 8, splice variant 2, chain 1, domain 1, sequence conflict 1, zinc finger region 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q86TJ2-F1	87.27	0.63

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (8): 45; 49; 9; 12; 23; 26; 32; 35

Function

Pathways and Gene Ontology

Reactome pathways

7 pathways

ID	Pathway
R-HSA-3214847	HATs acetylate histones
R-HSA-5689880	Ub-specific processing proteases
R-HSA-3247509	Chromatin modifying enzymes
R-HSA-392499	Metabolism of proteins
R-HSA-4839726	Chromatin organization
R-HSA-5688426	Deubiquitination
R-HSA-597592	Post-translational protein modification

MSigDB gene sets: 90 (showing top): IVANOVA_HEMATOPOIESIS_LATE_PROGENITOR, GOBP_REGULATION_OF_DNA_REPAIR, MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP, GOBP_REGULATION_OF_RESPONSE_TO_STRESS, GOBP_RNA_SPLICING, GOBP_DNA_DAMAGE_RESPONSE, chr4p16, GCM_DDX11, PID_P53_DOWNSTREAM_PATHWAY, GOBP_REGULATION_OF_CELLULAR_RESPONSE_TO_STRESS, GCM_NF2, CUI_TCF21_TARGETS_2_DN, GOBP_CHROMATIN_REMODELING, GOBP_REGULATION_OF_RNA_SPLICING, GOCC_SAGA_COMPLEX

GO Biological Process (5): regulation of DNA repair (GO:0006282), chromatin remodeling (GO:0006338), regulation of transcription by RNA polymerase II (GO:0006357), regulation of RNA splicing (GO:0043484), positive regulation of DNA-templated transcription (GO:0045893)

GO Molecular Function (5): chromatin binding (GO:0003682), transcription coactivator activity (GO:0003713), zinc ion binding (GO:0008270), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (4): SAGA complex (GO:0000124), nucleus (GO:0005634), nucleoplasm (GO:0005654), SAGA-type complex (GO:0070461)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
Chromatin modifying enzymes	1
Deubiquitination	1
Chromatin organization	1
Post-translational protein modification	1
Metabolism of proteins	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
regulation of DNA-templated transcription	2
binding	2
DNA repair	1
regulation of DNA metabolic process	1
regulation of cellular response to stress	1
chromatin organization	1
transcription by RNA polymerase II	1
RNA splicing	1
regulation of gene expression	1
regulation of primary metabolic process	1
DNA-templated transcription	1
positive regulation of RNA biosynthetic process	1
transcription coregulator activity	1
positive regulation of DNA-templated transcription	1
transition metal ion binding	1
cation binding	1
SAGA-type complex	1
DUBm complex	1
peptidase complex	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cellular anatomical structure	1
histone acetyltransferase complex	1

Protein interactions and networks

STRING

1304 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TADA2B	TADA3	O75528	989
TADA2B	SGF29	Q96ES7	982
TADA2B	TAF9	Q16594	945
TADA2B	KAT2A	Q92830	932
TADA2B	KAT2B	Q92831	907
TADA2B	SUPT3H	O75486	812
TADA2B	YEATS2	Q9ULM3	794
TADA2B	ENY2	Q9NPA8	787
TADA2B	SUPT7L	O94864	775
TADA2B	TRRAP	Q9Y4A5	763
TADA2B	VCF1	Q969W3	747
TADA2B	TAF6L	Q9Y6J9	739
TADA2B	SUPT20H	Q8NEM7	738
TADA2B	CCDC127	Q96BQ5	719
TADA2B	ATXN7	O15265	707

IntAct

67 interactions, top by confidence:

A	B	Type	Score
MED4	MED19	psi-mi:“MI:0914”(association)	0.900
SGF29	NDC80	psi-mi:“MI:0914”(association)	0.840
KPNA6	RNMT	psi-mi:“MI:0914”(association)	0.800
MED14	MED19	psi-mi:“MI:0914”(association)	0.790
MED9	MED19	psi-mi:“MI:0914”(association)	0.790
TAF12	TAF4	psi-mi:“MI:0914”(association)	0.760
TRRAP	ATXN7	psi-mi:“MI:0914”(association)	0.740
TADA1	TAF5L	psi-mi:“MI:0914”(association)	0.640
ATXN7L3	USP27X	psi-mi:“MI:0914”(association)	0.640
KPNA1	TCERG1	psi-mi:“MI:0914”(association)	0.640
TADA2B	AATF	psi-mi:“MI:0915”(physical association)	0.540
AATF	TADA2B	psi-mi:“MI:0915”(physical association)	0.540
AATF	TADA2B	psi-mi:“MI:0403”(colocalization)	0.540
TADA2B	SUPT3H	psi-mi:“MI:0914”(association)	0.530
MED29	POLR2D	psi-mi:“MI:0914”(association)	0.530
SUPT20H	ATXN7	psi-mi:“MI:0914”(association)	0.530
TAF6L	SUPT3H	psi-mi:“MI:0914”(association)	0.530
SGF29	MATN2	psi-mi:“MI:0914”(association)	0.530
H3C1	SMCHD1	psi-mi:“MI:2364”(proximity)	0.410
ECE1	TADA2B	psi-mi:“MI:0915”(physical association)	0.370
TADA2B	ECE1	psi-mi:“MI:0915”(physical association)	0.370

BioGRID (196): TADA2B (Affinity Capture-MS), TADA2B (Affinity Capture-MS), TADA2B (Affinity Capture-MS), TADA2B (Affinity Capture-MS), SUPT20H (Affinity Capture-MS), TERF2IP (Affinity Capture-MS), TRRAP (Affinity Capture-MS), TADA3 (Affinity Capture-MS), EPN1 (Affinity Capture-MS), TAF9B (Affinity Capture-MS), TAF9 (Affinity Capture-MS), TAF10 (Affinity Capture-MS), TERF2 (Affinity Capture-MS), SIRT1 (Affinity Capture-MS), TAF12 (Affinity Capture-MS)

ESM2 similar proteins: A0A2R8RWN9, A0JN27, E2R222, F1LTR1, O94925, P13264, P22234, P51583, P61201, P61202, P61203, P97834, Q07G17, Q13042, Q13888, Q15303, Q15645, Q28D01, Q2TBV1, Q2TBV5, Q2YDL1, Q3MHJ2, Q4R9A8, Q4VC33, Q5F398, Q5JUK3, Q5R532, Q5RB59, Q5RBN9, Q5RKJ1, Q5SP67, Q5TDH0, Q5XHZ9, Q61527, Q6GR10, Q6IQT4, Q6IR75, Q6P1K8, Q7L5Y9, Q7SXR3

Diamond homologs: Q503N9, Q5RBN9, Q6NRB5, Q7KSD8, Q86TJ2, Q8I8V0, Q9SFD5, Q54J55, O75478, Q3SZP8, Q54NA6, Q5ZJF3, Q6AYE3, Q8CHV6

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
TADA2B	“form complex”	“SAGA complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 62 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Respiratory Syncytial Virus Infection Pathway	6	25.1×	7e-06
HATs acetylate histones	13	21.9×	4e-12
RSV-host interactions	6	20.0×	2e-05
Adipogenesis	6	20.0×	2e-05
Epigenetic regulation by WDR5-containing histone modifying complexes	6	19.7×	2e-05
Chromatin organization	11	19.1×	8e-10
Chromatin modifying enzymes	12	18.4×	2e-10
Regulation of lipid metabolism by PPARalpha	6	18.0×	3e-05

GO biological processes:

GO term	Partners	Fold	FDR
regulation of DNA repair	15	69.1×	8e-22
regulation of RNA splicing	15	54.7×	2e-20
RNA polymerase II preinitiation complex assembly	10	45.3×	2e-12
positive regulation of transcription initiation by RNA polymerase II	9	40.8×	8e-11
positive regulation of transcription elongation by RNA polymerase II	7	35.1×	6e-08
transcription by RNA polymerase II	11	12.9×	5e-08

Disease & clinical

Clinical variants and AI predictions

ClinVar

32 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	29
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

433 predictions. Top by Δscore:

Variant	Effect	Δscore
4:7043847:TGGGT:T	donor_loss	1.0000
4:7043848:GG:G	donor_gain	0.9900
4:7043849:GG:G	donor_gain	0.9900
4:7043850:G:GC	donor_loss	0.9900
4:7043850:G:GG	donor_gain	0.9900
4:7043851:T:G	donor_loss	0.9900
4:7044245:G:T	donor_gain	0.9900
4:7054061:GGAA:G	acceptor_gain	0.9900
4:7043852:GA:G	donor_loss	0.9800
4:7054060:A:AG	acceptor_gain	0.9800
4:7054061:G:GG	acceptor_gain	0.9800
4:7043846:CTGG:C	donor_gain	0.9700
4:7054056:TCGTA:T	acceptor_loss	0.9700
4:7054057:CGTA:C	acceptor_loss	0.9700
4:7054058:GTA:G	acceptor_loss	0.9700
4:7054060:A:AC	acceptor_loss	0.9700
4:7054061:G:C	acceptor_loss	0.9700
4:7053431:A:T	donor_gain	0.9600
4:7043842:GAAAC:G	donor_gain	0.9500
4:7054061:GGA:G	acceptor_gain	0.9500
4:7043847:TGG:T	donor_gain	0.9400
4:7043848:GGG:G	donor_gain	0.9400
4:7044250:A:T	donor_gain	0.9400
4:7050822:G:GT	donor_gain	0.9300
4:7050822:G:T	donor_gain	0.9300
4:7054051:T:A	acceptor_loss	0.9300
4:7044245:G:GT	donor_gain	0.9100
4:7054055:A:AG	acceptor_gain	0.9100
4:7054057:C:CA	acceptor_gain	0.9100
4:7043845:ACTGG:A	donor_gain	0.8900

AlphaMissense

2765 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
4:7043604:T:C	C9R	1.000
4:7043605:G:A	C9Y	1.000
4:7043606:C:G	C9W	1.000
4:7043646:T:C	C23R	1.000
4:7043647:G:A	C23Y	1.000
4:7043648:C:G	C23W	1.000
4:7043673:T:C	C32R	1.000
4:7043682:T:A	C35S	1.000
4:7043682:T:C	C35R	1.000
4:7043683:G:C	C35S	1.000
4:7043787:T:A	W70R	1.000
4:7043787:T:C	W70R	1.000
4:7043820:G:C	A81P	1.000
4:7043842:G:A	G88E	1.000
4:7043847:T:A	W90R	1.000
4:7043847:T:C	W90R	1.000
4:7043849:G:C	W90C	1.000
4:7043849:G:T	W90C	1.000
4:7054300:G:C	R170P	1.000
4:7054429:T:C	L213P	1.000
4:7054439:A:C	R216S	1.000
4:7054439:A:T	R216S	1.000
4:7054447:G:C	R219P	1.000
4:7054663:T:C	L291P	1.000
4:7054675:G:C	R295P	1.000
4:7054855:A:T	E355V	1.000
4:7054868:C:G	C359W	1.000
4:7054910:G:C	K373N	1.000
4:7054910:G:T	K373N	1.000
4:7055017:T:C	L409P	1.000

dbSNP variants (sampled 300 via entrez): RS1000320286 (4:7051204 G>A), RS1000346404 (4:7042451 T>C), RS1000357659 (4:7056998 C>T), RS1000385053 (4:7056686 G>A), RS1000404803 (4:7056060 T>C), RS1000685959 (4:7058065 G>A,C), RS1000716781 (4:7057894 A>G), RS1000855465 (4:7047114 C>G,T), RS1000881456 (4:7052454 G>A), RS1000906214 (4:7052155 C>T), RS1001055777 (4:7047665 G>A), RS10012093 (4:7046096 T>A,C,G), RS1001396147 (4:7052322 C>T), RS1001450283 (4:7058315 G>C,T), RS1001475937 (4:7057284 G>A)

Disease associations

OMIM: gene MIM:608790 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

12 associations (top):

Study	Trait	p-value
GCST004607_271	Plateletcrit	4.000000e-14
GCST004613_102	Sum neutrophil eosinophil counts	8.000000e-10
GCST004614_145	Granulocyte count	1.000000e-09
GCST004620_139	Sum basophil neutrophil counts	1.000000e-09
GCST004625_80	Monocyte count	8.000000e-29
GCST004626_33	Myeloid white cell count	5.000000e-12
GCST004629_57	Neutrophil count	7.000000e-10
GCST004632_99	Lymphocyte percentage of white cells	7.000000e-14
GCST90002393_218	Monocyte count	2.000000e-52
GCST90002394_272	Monocyte percentage of white cells	8.000000e-29
GCST90002400_423	Plateletcrit	4.000000e-39
GCST90002402_678	Platelet count	5.000000e-17

EFO canonical traits (9, from GWAS)

EFO ID	Trait name
EFO:0007985	platelet crit
EFO:0004833	neutrophil count
EFO:0004842	eosinophil count
EFO:0007987	granulocyte count
EFO:0005090	basophil count
EFO:0005091	monocyte count
EFO:0007993	lymphocyte percentage of leukocytes
EFO:0007989	monocyte percentage of leukocytes
EFO:0004309	platelet count

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

14 total (human), top 14 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects expression, increases expression	2
triphenyl phosphate	affects expression	1
butyraldehyde	decreases expression	1
manganese chloride	decreases expression, increases abundance	1
Sunitinib	increases expression	1
Estradiol	decreases expression	1
Folic Acid	decreases expression	1
Formaldehyde	decreases expression	1
Manganese	decreases expression, increases abundance	1
Smoke	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Tretinoin	increases expression	1
Urethane	increases expression	1
Cadmium Chloride	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.