TBKBP1

Summary

TBKBP1 (TBK1 binding protein 1, HGNC:30140) is a protein-coding gene on chromosome 17q21.32, encoding TANK-binding kinase 1-binding protein 1 (A7MCY6). Adapter protein which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity.

TBKBP1 is an adaptor protein that binds to TBK1 (MIM 604834) and is part of the interaction network in the TNF (MIM 191160)/NFKB (see MIM 164011) pathway (Bouwmeester et al., 2004 [PubMed 14743216]).

Source: NCBI Gene 9755 — RefSeq curated summary.

At a glance

• GWAS associations: 13
• Clinical variants (ClinVar): 131 total
• Druggable target: yes
• MANE Select transcript: NM_001394755

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30140
Approved symbol	TBKBP1
Name	TBK1 binding protein 1
Location	17q21.32
Locus type	gene with protein product
Status	Approved
Aliases	ProSAPiP2, KIAA0775
Ensembl gene	ENSG00000198933
Ensembl biotype	protein_coding
OMIM	608476
Entrez	9755

Gene structure

Transcript identifiers

Ensembl transcripts: 13 — 13 protein_coding

ENST00000361722, ENST00000537587, ENST00000578982, ENST00000851180, ENST00000851181, ENST00000851182, ENST00000851183, ENST00000851184, ENST00000851185, ENST00000938873, ENST00000938874, ENST00000938875, ENST00000938876

RefSeq mRNA: 3 — MANE Select: NM_001394755 NM_001394755, NM_001394756, NM_014726

CCDS: CCDS45722

Canonical transcript exons

ENST00000578982 — 10 exons

Exon	Start	End
ENSE00000924999	47696711	47696833
ENSE00000925000	47697089	47697193
ENSE00000925001	47698595	47698775
ENSE00000925002	47699320	47699495
ENSE00000925003	47699636	47699697
ENSE00000925004	47708394	47708512
ENSE00001137906	47708725	47709452
ENSE00002695017	47696079	47696337
ENSE00002728589	47694161	47694194
ENSE00003934568	47710498	47712052

Expression profiles

Bgee: expression breadth ubiquitous, 190 present calls, max score 91.06.

FANTOM5 (CAGE): breadth broad, TPM avg 1.2935 / max 31.1376, expressed in 678 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
161370	1.2935	678

Top tissues by expression

258 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
cingulate cortex	UBERON:0003027	91.06	gold quality
right frontal lobe	UBERON:0002810	91.01	gold quality
anterior cingulate cortex	UBERON:0009835	90.97	gold quality
apex of heart	UBERON:0002098	89.89	gold quality
diaphragm	UBERON:0001103	89.80	gold quality
amygdala	UBERON:0001876	88.67	gold quality
gastrocnemius	UBERON:0001388	88.50	gold quality
cortical plate	UBERON:0005343	87.66	gold quality
muscle of leg	UBERON:0001383	87.61	gold quality
granulocyte	CL:0000094	87.58	gold quality
right hemisphere of cerebellum	UBERON:0014890	87.29	gold quality
hindlimb stylopod muscle	UBERON:0004252	87.24	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	87.22	gold quality
prefrontal cortex	UBERON:0000451	86.94	gold quality
neocortex	UBERON:0001950	86.78	gold quality
cerebellar hemisphere	UBERON:0002245	86.60	gold quality
right ovary	UBERON:0002118	86.51	gold quality
cerebellar cortex	UBERON:0002129	86.51	gold quality
Brodmann (1909) area 9	UBERON:0013540	86.41	gold quality
frontal cortex	UBERON:0001870	86.40	gold quality
left ovary	UBERON:0002119	86.01	gold quality
cerebral cortex	UBERON:0000956	85.68	gold quality
muscle organ	UBERON:0001630	85.63	gold quality
cerebellum	UBERON:0002037	85.56	gold quality
hypothalamus	UBERON:0001898	85.39	gold quality
Ammon’s horn	UBERON:0001954	85.29	gold quality
olfactory bulb	UBERON:0002264	85.11	gold quality
right atrium auricular region	UBERON:0006631	84.89	gold quality
heart left ventricle	UBERON:0002084	84.78	gold quality
cardiac ventricle	UBERON:0002082	84.48	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.21

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

114 targeting TBKBP1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6748-5P	100.00	65.81	1057
HSA-MIR-6740-5P	100.00	65.64	932
HSA-MIR-4283	100.00	66.42	2097
HSA-MIR-6851-5P	100.00	65.63	1294
HSA-MIR-3689D	100.00	66.14	1181
HSA-MIR-4747-5P	100.00	67.90	2681
HSA-MIR-5196-5P	100.00	67.98	2761
HSA-MIR-4500	99.99	72.72	2367
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-10401-5P	99.99	65.79	948
HSA-MIR-103A-3P	99.98	69.14	1595
HSA-MIR-107	99.98	69.14	1595
HSA-LET-7A-5P	99.98	72.29	1790
HSA-LET-7B-5P	99.98	72.31	1790
HSA-LET-7C-5P	99.98	72.29	1790
HSA-LET-7E-5P	99.98	72.29	1790
HSA-LET-7F-5P	99.98	72.56	1784
HSA-LET-7G-5P	99.98	72.37	1784
HSA-LET-7I-5P	99.98	72.37	1788
HSA-MIR-98-5P	99.98	72.33	1787
HSA-LET-7D-5P	99.96	71.76	1632
HSA-MIR-4458	99.96	71.64	1650
HSA-MIR-6825-5P	99.96	69.81	3431
HSA-MIR-185-3P	99.95	67.01	1743
HSA-MIR-10523-5P	99.91	69.22	2038
HSA-MIR-7162-3P	99.89	68.16	1682
HSA-MIR-12119	99.87	68.35	1653
HSA-MIR-4728-5P	99.85	69.39	4718
HSA-MIR-202-3P	99.84	71.41	1290
HSA-MIR-6785-5P	99.82	68.68	4428

Literature-anchored findings (GeneRIF, showing 8)

• results suggest that efficient signal transduction upon viral infection requires SINTBAD, TANK and NAP1 because they link TBK1 and IKKi to virus-activated signalling cascades (PMID:17568778)
• Studies show that three proteins expressed in HEK-293T cells (NAP1, TANK and TBKBP1) interact with TBK1. (PMID:23286385)
• TBKBP1 is associated with the severity of ankylosing spondylitis in the Chinese Han population (PMID:23637848)
• The two selected single nucleotide polymorphisms (SNPs) in TBKBP1 show no relationship with the clinical outcomes. None of the 12 SNPs is related to mSASSS. (PMID:25494292)
• we show that, essential for anti-bacterial autophagy, the cargo receptor NDP52 forms a trimeric complex with FIP200 and SINTBAD/NAP1, which are subunits of the autophagy-initiating ULK and the TBK1 kinase complex, respectively. FIP200 and SINTBAD/NAP1 are each recruited independently to bacteria via NDP52, as revealed by selective point mutations in their respective binding sites (PMID:30853402)
• Analysis of 47 Non-MHC Ankylosing Spondylitis Susceptibility Loci Regarding Associated Variants across Whites and Han Chinese. (PMID:31523044)
• The TBK1-TBKBP1 axis facilitates tumour-mediated immunosuppression. (PMID:31792381)
• A multi-step genomic approach prioritized TBKBP1 gene as relevant for multiple sclerosis susceptibility. (PMID:35545683)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	tbkbp1	ENSDARG00000061473
mus_musculus	Tbkbp1	ENSMUSG00000038517
rattus_norvegicus	Tbkbp1	ENSRNOG00000009370

Paralogs (1): AZI2 (ENSG00000163512)

Protein

Protein identifiers

TANK-binding kinase 1-binding protein 1 — A7MCY6 (reviewed: A7MCY6)

All UniProt accessions (3): A7MCY6, F5H1U4, J3KS71

UniProt curated annotations — full annotation on UniProt →

Function. Adapter protein which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity.

Subunit / interactions. Homodimer. May form a heterodimer with NAP1. Interacts with TKB1 and IKBKE. Weakly interacts with DDX3X. (Microbial infection) Interacts with vaccinia virus protein C6.

Tissue specificity. Detected in leukocytes, lung, placenta, small intestine, liver, kidney, spleen, muscle, heart, brain and at low levels in thymus.

Isoforms (2)

UniProt ID	Names	Canonical?
A7MCY6-1	1	yes
A7MCY6-2	2

RefSeq proteins (3): NP_001381684, NP_001381685, NP_055541 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR024581	TBD	Domain
IPR041641	CALCOCO1/2_Zn_UBZ1	Domain
IPR051891	TBK1-IKBKE_adapters	Family

Pfam: PF12845

UniProt features (25 total): modified residue 9, binding site 4, region of interest 3, compositionally biased region 3, splice variant 2, coiled-coil region 2, chain 1, zinc finger region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A7MCY6-F1	64.49	0.31

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 586; 589; 605; 609

Post-translational modifications (9): 184, 365, 372, 379, 385, 400, 415, 504, 534

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 111 (showing top): GOBP_RESPONSE_TO_PEPTIDE, ATACCTC_MIR202, GOBP_RESPONSE_TO_TYPE_I_INTERFERON, GOBP_DEFENSE_RESPONSE_TO_OTHER_ORGANISM, BASSO_HAIRY_CELL_LEUKEMIA_UP, GOBP_DEFENSE_RESPONSE_TO_VIRUS, KEGG_RIG_I_LIKE_RECEPTOR_SIGNALING_PATHWAY, CAGCCTC_MIR4855P, GOBP_RESPONSE_TO_VIRUS, GOCC_TRANSFERASE_COMPLEX_TRANSFERRING_PHOSPHORUS_CONTAINING_GROUPS, GOCC_TRANSFERASE_COMPLEX, GOCC_PROTEIN_KINASE_COMPLEX, MIKKELSEN_ES_ICP_WITH_H3K4ME3, MIKKELSEN_NPC_ICP_WITH_H3K4ME3, BHAT_ESR1_TARGETS_NOT_VIA_AKT1_UP

GO Biological Process (4): defense response to virus (GO:0051607), type I interferon-mediated signaling pathway (GO:0060337), immune system process (GO:0002376), innate immune response (GO:0045087)

GO Molecular Function (3): zinc ion binding (GO:0008270), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (2): cytoplasm (GO:0005737), serine/threonine protein kinase complex (GO:1902554)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
defense response	1
response to virus	1
cellular response to type I interferon	1
interferon-mediated signaling pathway	1
biological_process	1
immune response	1
defense response to symbiont	1
transition metal ion binding	1
binding	1
cation binding	1
intracellular anatomical structure	1
cellular anatomical structure	1
protein kinase complex	1

Protein interactions and networks

STRING

490 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TBKBP1	TBK1	Q9UHD2	994
TBKBP1	TANK	Q92844	985
TBKBP1	CALCOCO2	Q13137	944
TBKBP1	IKBKE	Q14164	936
TBKBP1	TRAF3	Q13114	770
TBKBP1	RB1CC1	Q8TDY2	738
TBKBP1	MAVS	Q7Z434	637
TBKBP1	TAX1BP1	Q86VP1	595
TBKBP1	AZI2	Q9H6S1	595
TBKBP1	CHUK	O15111	580
TBKBP1	IKBKB	O14920	576
TBKBP1	NFKB1	P19838	572
TBKBP1	TRADD	Q15628	534
TBKBP1	IRF3	Q14653	507
TBKBP1	BIRC2	Q13490	480

IntAct

44 interactions, top by confidence:

A	B	Type	Score
TBK1	TBKBP1	psi-mi:“MI:0914”(association)	0.860
TBKBP1	TBK1	psi-mi:“MI:0915”(physical association)	0.860
TBK1	TBKBP1	psi-mi:“MI:0915”(physical association)	0.860
TBKBP1	IKBKE	psi-mi:“MI:0914”(association)	0.720
IKBKE	TBKBP1	psi-mi:“MI:0915”(physical association)	0.720
TBKBP1	IKBKE	psi-mi:“MI:0915”(physical association)	0.720
CALCOCO2	TBKBP1	psi-mi:“MI:0914”(association)	0.640
rep	TBKBP1	psi-mi:“MI:0914”(association)	0.530
PICK1	ILVBL	psi-mi:“MI:0914”(association)	0.530
PIP	TBKBP1	psi-mi:“MI:0914”(association)	0.530
TBKBP1	OPG029	psi-mi:“MI:0915”(physical association)	0.520
OPG029	TBKBP1	psi-mi:“MI:0915”(physical association)	0.520
TBKBP1	APBA3	psi-mi:“MI:0915”(physical association)	0.500
RB1CC1	TBKBP1	psi-mi:“MI:0915”(physical association)	0.500
TBKBP1	AGO2	psi-mi:“MI:0915”(physical association)	0.500
TBKBP1	TBKBP1	psi-mi:“MI:0915”(physical association)	0.400
TBKBP1		psi-mi:“MI:0915”(physical association)	0.370
	TBKBP1	psi-mi:“MI:0914”(association)	0.350
EGLN3	FAM168B	psi-mi:“MI:0914”(association)	0.350
rep	TBKBP1	psi-mi:“MI:0914”(association)	0.350
CSNK2A2	VWA8	psi-mi:“MI:0914”(association)	0.350
MIF	BLTP3B	psi-mi:“MI:0914”(association)	0.350
POLR1D	BDP1	psi-mi:“MI:0914”(association)	0.350

BioGRID (113): TBKBP1 (Affinity Capture-MS), TBKBP1 (Affinity Capture-Western), TBKBP1 (Affinity Capture-MS), TBKBP1 (Affinity Capture-MS), TBKBP1 (Affinity Capture-RNA), TBKBP1 (Affinity Capture-MS), TBKBP1 (Affinity Capture-MS), TBKBP1 (Affinity Capture-Western), TBKBP1 (Affinity Capture-MS), TBKBP1 (Affinity Capture-MS), TBKBP1 (Affinity Capture-Western), TBKBP1 (Affinity Capture-Western), TBKBP1 (Proximity Label-MS), TBKBP1 (Proximity Label-MS), TBKBP1 (Proximity Label-MS)

ESM2 similar proteins: A0A0U1RQ45, A0A1B0GWB2, A2A9T0, A6QPA0, A7MCY6, D3ZFB6, E9PUL5, E9Q0B3, F5GYI3, F5H4A9, J3QNX5, O70142, P0C1G7, P81408, P97764, P98077, Q148V8, Q15654, Q2KI80, Q3SX26, Q3SZL6, Q4V9L6, Q5FVJ4, Q5FW56, Q5RAC1, Q5T7N3, Q6DG50, Q6PAJ3, Q6PJ61, Q6ZMQ8, Q6ZNR0, Q6ZRV2, Q75VX8, Q7Z6L0, Q86UK7, Q86VE0, Q8BGW2, Q8BRJ3, Q8BX43, Q8C0R7

Diamond homologs: A2A9T0, A7MCY6, Q3SYW5, Q4KMA0, Q4R3X1, Q5RD40, Q6DG50, Q9H6S1, Q9QYP6

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
TBKBP1	“up-regulates activity”	TBK1	relocalization
TBKBP1	“up-regulates activity”	CARD10	relocalization

Disease & clinical

Clinical variants and AI predictions

ClinVar

131 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	108
Likely benign	6
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

1541 predictions. Top by Δscore:

Variant	Effect	Δscore
17:47696337:GGTCA:G	donor_loss	1.0000
17:47696338:G:GA	donor_loss	1.0000
17:47696709:A:AG	acceptor_gain	1.0000
17:47696710:G:GA	acceptor_gain	1.0000
17:47696710:GTAC:G	acceptor_gain	1.0000
17:47696710:GTACC:G	acceptor_gain	1.0000
17:47696831:GAG:G	donor_gain	1.0000
17:47696832:AGGT:A	donor_loss	1.0000
17:47696834:G:GA	donor_loss	1.0000
17:47696835:T:A	donor_loss	1.0000
17:47697075:T:A	acceptor_gain	1.0000
17:47697084:T:G	acceptor_gain	1.0000
17:47697182:C:G	donor_gain	1.0000
17:47697187:G:GT	donor_gain	1.0000
17:47697190:GATG:G	donor_gain	1.0000
17:47697224:G:GT	donor_gain	1.0000
17:47698592:CA:C	acceptor_loss	1.0000
17:47698593:A:AG	acceptor_gain	1.0000
17:47698593:AGAG:A	acceptor_gain	1.0000
17:47698593:AGAGG:A	acceptor_gain	1.0000
17:47698594:G:GG	acceptor_gain	1.0000
17:47698594:GA:G	acceptor_gain	1.0000
17:47698594:GAGG:G	acceptor_gain	1.0000
17:47698594:GAGGG:G	acceptor_gain	1.0000
17:47698670:G:GT	donor_gain	1.0000
17:47698764:GCT:G	donor_gain	1.0000
17:47699319:GCA:G	acceptor_gain	1.0000
17:47699496:GT:G	donor_loss	1.0000
17:47699497:T:G	donor_loss	1.0000
17:47699630:CCTTA:C	acceptor_loss	1.0000

AlphaMissense

1273 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
17:47696243:T:C	L44P	0.999
17:47696254:T:G	Y48D	0.999
17:47696243:T:A	L44H	0.998
17:47696252:C:A	A47D	0.998
17:47696268:G:C	K52N	0.998
17:47696268:G:T	K52N	0.998
17:47696276:T:C	L55P	0.998
17:47696318:T:C	L69P	0.998
17:47696251:G:C	A47P	0.997
17:47696264:T:A	I51N	0.997
17:47696273:G:C	R54P	0.997
17:47696254:T:C	Y48H	0.996
17:47696264:T:G	I51S	0.996
17:47696306:T:C	L65P	0.996
17:47696240:C:A	A43D	0.995
17:47696254:T:A	Y48N	0.995
17:47696285:T:C	L58P	0.995
17:47697183:T:C	L148P	0.995
17:47696264:T:C	I51T	0.993
17:47697137:G:C	A133P	0.993
17:47697150:T:C	L137P	0.993
17:47696237:T:C	F42S	0.992
17:47696236:T:C	F42L	0.991
17:47696238:T:A	F42L	0.991
17:47696238:T:G	F42L	0.991
17:47696243:T:G	L44R	0.990
17:47696246:T:A	I45N	0.990
17:47696296:A:G	N62D	0.990
17:47696255:A:C	Y48S	0.989
17:47696276:T:A	L55Q	0.989

dbSNP variants (sampled 300 via entrez): RS1000015091 (17:47694896 G>A,C,T), RS1000310516 (17:47700268 A>C,G,T), RS1000311683 (17:47711634 C>T), RS1000536049 (17:47705898 G>A), RS1000690374 (17:47712542 G>A), RS1000785705 (17:47711215 C>T), RS1000889667 (17:47711932 C>G,T), RS1000907053 (17:47694588 C>A,T), RS1000939098 (17:47694440 G>A), RS1001040628 (17:47706140 G>C), RS1001121773 (17:47699695 T>C), RS1001365473 (17:47694298 C>T), RS1001418444 (17:47693692 C>T), RS1001513276 (17:47706098 T>G), RS1001890903 (17:47712099 C>A,G,T)

Disease associations

OMIM: gene MIM:608476 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

13 associations (top):

Study	Trait	p-value
GCST001149_8	Ankylosing spondylitis	5.000000e-08
GCST001341_8	Multiple sclerosis	1.000000e-07
GCST002690_12	Very long-chain saturated fatty acid levels (fatty acid 20:0)	4.000000e-07
GCST004557_34	Body mass index	2.000000e-08
GCST004558_1	Body mass index (joint analysis main effects and physical activity interaction)	3.000000e-09
GCST004560_22	Body mass index in physically inactive individuals	3.000000e-06
GCST005529_24	Ankylosing spondylitis	2.000000e-12
GCST005529_42	Ankylosing spondylitis	6.000000e-15
GCST005951_16	Body mass index	4.000000e-08
GCST007995_4	Asthma (childhood onset)	3.000000e-09
GCST008916_117	Asthma	1.000000e-09
GCST90002390_115	Mean corpuscular hemoglobin	1.000000e-11
GCST90006909_3	Human herpes virus 7 U14 antibody levels	7.000000e-09

EFO canonical traits (5, from GWAS)

EFO ID	Trait name
EFO:0006796	very long-chain saturated fatty acid measurement
EFO:0004340	body mass index
EFO:0008002	physical activity measurement
EFO:0004527	mean corpuscular hemoglobin
EFO:0011038	anti-human herpes virus 7 antibody measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4523108 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 5 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.32	Kd	47.61	nM	CHEMBL5653589
7.22	ED50	59.99	nM	CHEMBL5653589
5.68	Kd	2090	nM	CHEMBL4462318
5.04	Kd	9065	nM	CHEMBL3752910

PubChem BioAssay actives

3 with measured affinity, of 5 total; 3 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149556: Binding affinity to human TBKBP1 incubated for 45 mins by Kinobead based pull down assay	kd	0.0476	uM
2,6-difluoro-N-[3-fluoro-4-[6-methoxy-7-[3-(4-methylpiperazin-1-yl)propoxy]quinolin-4-yl]oxyphenyl]benzenesulfonamide	1573324: Binding affinity to TBKBP1 in SILAC-labeled human MDA-MB-231 cells lysate by mass spectrometry based kinAffinity assay	kd	2.0900	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149556: Binding affinity to human TBKBP1 incubated for 45 mins by Kinobead based pull down assay	kd	9.0650	uM

CTD chemical–gene interactions

29 total (human), top 29 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Estradiol	affects binding, increases expression	2
Tobacco Smoke Pollution	increases expression, increases methylation	2
aristolochic acid I	increases expression	1
FR900359	increases phosphorylation	1
TL8-506	affects cotreatment, increases expression	1
dicrotophos	increases expression	1
triphenyl phosphate	affects expression	1
beta-lapachone	increases expression	1
sodium arsenite	decreases expression	1
diallyl trisulfide	increases expression	1
tamibarotene	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
CGP 52608	affects binding, increases reaction	1
monomethylarsonous acid	increases expression	1
(+)-JQ1 compound	decreases expression	1
Sunitinib	increases expression	1
Air Pollutants	increases abundance, increases expression	1
Benzo(a)pyrene	decreases methylation	1
Cisplatin	decreases expression	1
Dichlorodiphenyl Dichloroethylene	decreases expression	1
Lead	affects expression	1
Methotrexate	decreases expression	1
Smoke	decreases expression	1
Tetrachlorodibenzodioxin	increases expression	1
Tretinoin	decreases expression	1
Urethane	decreases expression	1
Valproic Acid	increases methylation	1
Okadaic Acid	decreases expression	1
Particulate Matter	increases abundance, increases expression	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4371454	Binding	Binding affinity to TBKBP1 in SILAC-labeled human MDA-MB-231 cells lysate by mass spectrometry based kinAffinity assay	Discovery of N-[4-(Quinolin-4-yloxy)phenyl]benzenesulfonamides as Novel AXL Kinase Inhibitors. — J Med Chem

Cellosaurus cell lines

14 cell lines: 14 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2I8	Abcam HeLa TBKBP1 KO	Cancer cell line	Female
CVCL_C8QB	HeLa S3 penta KO-AZI2/TBKBP1 DKO clone 20	Cancer cell line	Female
CVCL_C9DV	HeLa S3 AZI2/TBKBP1 DKO clone 13	Cancer cell line	Female
CVCL_C9DW	HeLa S3 penta KO-ULK1/ULK2 DKO-AZI2/TBKBP1 DKO	Cancer cell line	Female
CVCL_D2Q0	HeLa S3 AZI2/TBKBP1 DKO clone 14	Cancer cell line	Female
CVCL_D2Q1	HeLa S3 penta KO-AZI2/TBKBP1 DKO clone 26	Cancer cell line	Female
CVCL_D2Y9	HeLa S3 TBKBP1 KO clone 7	Cancer cell line	Female
CVCL_E2LK	HAP1 TBKBP1 (-) 3	Cancer cell line	Male
CVCL_E2LL	HAP1 TBKBP1 (-) 4	Cancer cell line	Male
CVCL_E2LM	HAP1 TBKBP1 (-) 5	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.