TBRG4

Summary

TBRG4 (transforming growth factor beta regulator 4, HGNC:17443) is a protein-coding gene on chromosome 7p13, encoding FAST kinase domain-containing protein 4 (Q969Z0). Plays a role in processing of mitochondrial RNA precursors and in stabilization of a subset of mature mitochondrial RNA species, such as MT-CO1, MT-CO2, MT-CYB, MT-CO3, MT-ND3, MT-ND5 and MT-ATP8/6.

Enables RNA binding activity. Involved in mitochondrial mRNA processing and regulation of mitochondrial mRNA stability. Located in mitochondrial matrix.

Source: NCBI Gene 9238 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 125 total
• Druggable target: yes
• MANE Select transcript: NM_004749

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:17443
Approved symbol	TBRG4
Name	transforming growth factor beta regulator 4
Location	7p13
Locus type	gene with protein product
Status	Approved
Aliases	Cpr2, KIAA0948, H_TD2522F11.8, FASTKD4
Ensembl gene	ENSG00000136270
Ensembl biotype	protein_coding
OMIM	611325
Entrez	9238

Gene structure

Transcript identifiers

Ensembl transcripts: 65 — 58 protein_coding, 6 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000258770, ENST00000361278, ENST00000395655, ENST00000461363, ENST00000471142, ENST00000475893, ENST00000477328, ENST00000478116, ENST00000478532, ENST00000482285, ENST00000482482, ENST00000483615, ENST00000484326, ENST00000488222, ENST00000494076, ENST00000495078, ENST00000495973, ENST00000895097, ENST00000895098, ENST00000895099, ENST00000895100, ENST00000895101, ENST00000895102, ENST00000895103, ENST00000895104, ENST00000895105, ENST00000895106, ENST00000895107, ENST00000895108, ENST00000895109, ENST00000895110, ENST00000895111, ENST00000895112, ENST00000895113, ENST00000895114, ENST00000895115, ENST00000934796, ENST00000934797, ENST00000934798, ENST00000934799, ENST00000934800, ENST00000934801, ENST00000934802, ENST00000934803, ENST00000934804, ENST00000934805, ENST00000934806, ENST00000934807, ENST00000934808, ENST00000934809, ENST00000934810, ENST00000934811, ENST00000934812, ENST00000967962, ENST00000967963, ENST00000967964, ENST00000967965, ENST00000967966, ENST00000967967, ENST00000967968, ENST00000967969, ENST00000967970, ENST00000967971, ENST00000967972, ENST00000967973

RefSeq mRNA: 4 — MANE Select: NM_004749 NM_001261834, NM_004749, NM_030900, NM_199122

CCDS: CCDS5501, CCDS5502

Canonical transcript exons

ENST00000258770 — 11 exons

Exon	Start	End
ENSE00001391193	45105441	45105764
ENSE00001873350	45111643	45111697
ENSE00001893480	45100100	45100426
ENSE00003516026	45108827	45109287
ENSE00003522323	45102347	45102491
ENSE00003528137	45104099	45104256
ENSE00003540784	45101258	45101372
ENSE00003653705	45103333	45103443
ENSE00003659889	45101825	45102070
ENSE00003785748	45104538	45104709
ENSE00003791097	45101503	45101614

Expression profiles

Bgee: expression breadth ubiquitous, 214 present calls, max score 96.83.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 32.5868 / max 394.3942, expressed in 1817 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
83934	32.5868	1817

Top tissues by expression

284 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
mucosa of transverse colon	UBERON:0004991	96.83	gold quality
apex of heart	UBERON:0002098	96.80	gold quality
transverse colon	UBERON:0001157	96.04	gold quality
granulocyte	CL:0000094	95.56	gold quality
small intestine Peyer’s patch	UBERON:0003454	95.41	gold quality
gastrocnemius	UBERON:0001388	95.29	gold quality
metanephros cortex	UBERON:0010533	95.22	gold quality
rectum	UBERON:0001052	95.03	gold quality
muscle of leg	UBERON:0001383	94.67	gold quality
hindlimb stylopod muscle	UBERON:0004252	94.52	gold quality
lower esophagus mucosa	UBERON:0035834	94.51	gold quality
skin of leg	UBERON:0001511	94.40	gold quality
right ovary	UBERON:0002118	94.36	gold quality
body of stomach	UBERON:0001161	94.35	gold quality
minor salivary gland	UBERON:0001830	94.25	gold quality
right adrenal gland	UBERON:0001233	94.24	gold quality
right lobe of liver	UBERON:0001114	94.20	gold quality
right adrenal gland cortex	UBERON:0035827	94.18	gold quality
muscle layer of sigmoid colon	UBERON:0035805	94.17	gold quality
skin of abdomen	UBERON:0001416	94.15	gold quality
lower esophagus muscularis layer	UBERON:0035833	94.12	gold quality
lower esophagus	UBERON:0013473	94.11	gold quality
right lobe of thyroid gland	UBERON:0001119	94.05	gold quality
left ovary	UBERON:0002119	93.98	gold quality
esophagus mucosa	UBERON:0002469	93.96	gold quality
heart left ventricle	UBERON:0002084	93.89	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	93.83	gold quality
right uterine tube	UBERON:0001302	93.82	gold quality
esophagus	UBERON:0001043	93.81	gold quality
right atrium auricular region	UBERON:0006631	93.73	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.42

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

8 targeting TBRG4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-122B-3P	99.21	68.90	1333
HSA-MIR-21-3P	99.21	68.95	1312
HSA-MIR-12135	98.99	70.26	1814
HSA-MIR-4801	98.96	69.42	2096
HSA-MIR-3190-5P	98.87	64.89	1345
HSA-MIR-4731-3P	98.56	68.60	1860
HSA-MIR-2355-3P	96.84	68.54	909

Literature-anchored findings (GeneRIF, showing 9)

• Disruption of FASTKD4 reduces the level of ND3 and of other mature mRNAs including ND5 and CYB, and causes accumulation of ND5-CYB precursor RNA. Disrupting FASTKD1 and FASTKD4 decreases ND3 mRNA like depleting FASTKD4 alone, indicating that FASTKD4 loss is epistatic. RAP domain of FASTKD4 has a nuclease fold with a conserved aspartate at the putative active site, mutation of which abolishes its function. (PMID:28335001)
• SLERT is 694 nucleotides & contains SNORA5A & SNORA5C at its ends; it is produced from the human TBRG4 locus via skipping of exons 4 and 5 that leads to both SNORA5A & SNORA5C embedded within one intron, thereby producing SLERT from this alternatively spliced intron; results reveal an important control of ribosome biogenesis by SLERT lncRNA & its regulatory role in DDX21 ring-shaped arrangements acting on Pol I complexes. (PMID:28475895)
• Despite the fact that proteins of the FASTK family FASTKD1-5 share the same domains, they exhibit various-sometimes opposing-functions in almost all steps of mitochondrial RNA metabolism. (PMID:29036396)
• TBRG4 silencing promotes progression of squamous cell carcinoma via regulation of CAV-1 expression and ROS formation. (PMID:32415943)
• Expression quantitative trait loci of genes predicting outcome are associated with survival of multiple myeloma patients. (PMID:33675538)
• The FASTK family proteins fine-tune mitochondrial RNA processing. (PMID:34748562)
• Mitochondrial protein, TBRG4, modulates KSHV and EBV reactivation from latency. (PMID:36417478)
• High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma. (PMID:38347489)
• 13 human CPR (cell cycle progression restoration) genes and 11 yeast OPY (overproduction-induced pheromone-resistant yeast) genes that specifically block the G1 arrest by mating pheromone. (PMID:9383053)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	tbrg4	ENSDARG00000074807
mus_musculus	Tbrg4	ENSMUSG00000000384
rattus_norvegicus	Tbrg4	ENSRNOG00000052477
caenorhabditis_elegans		WBGENE00007207

Paralogs (5): FASTKD2 (ENSG00000118246), FASTKD3 (ENSG00000124279), FASTKD1 (ENSG00000138399), FASTK (ENSG00000164896), FASTKD5 (ENSG00000215251)

Protein

Protein identifiers

FAST kinase domain-containing protein 4 — Q969Z0 (reviewed: Q969Z0)

Alternative names: Cell cycle progression restoration protein 2, Protein TBRG4, Transforming growth factor beta regulator 4

All UniProt accessions (7): C9IZN7, C9J347, C9J5A2, C9J618, C9J7P5, Q969Z0, H7C4R5

UniProt curated annotations — full annotation on UniProt →

Function. Plays a role in processing of mitochondrial RNA precursors and in stabilization of a subset of mature mitochondrial RNA species, such as MT-CO1, MT-CO2, MT-CYB, MT-CO3, MT-ND3, MT-ND5 and MT-ATP8/6. May play a role in cell cycle progression.

Subcellular location. Mitochondrion matrix.

Tissue specificity. Ubiquitously expressed. Expression detected in spleen, thymus, testis, ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and white adipose tissue with highest expression in smooth muscle.

Domain organisation. RAP domain is required for TBRG4 function in mRNA stability and translation.

Similarity. Belongs to the FAST kinase family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q969Z0-1	1	yes
Q969Z0-2	2

RefSeq proteins (4): NP_001248763, NP_004740, NP_112162, NP_954573 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR010622	FAST_Leu-rich	Domain
IPR013579	FAST_2	Domain
IPR013584	RAP	Domain
IPR050870	FAST_kinase	Family

Pfam: PF06743, PF08368, PF08373

UniProt features (11 total): sequence conflict 3, sequence variant 2, transit peptide 1, chain 1, domain 1, modified residue 1, splice variant 1, mutagenesis site 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
9GEK	X-RAY DIFFRACTION	2.15

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q969Z0-F1	83.29	0.51

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 553

Mutagenesis-validated functional residues (1):

Position	Phenotype
531	does not affect location in mitochondria; fails to rescue the increased accumulation of the level of mature mt-nd3, mt-c

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-9837092	FASTK family proteins regulate processing and stability of mitochondrial RNAs

MSigDB gene sets: 179 (showing top): GSE45365_NK_CELL_VS_CD8A_DC_DN, GOBP_REGULATION_OF_MRNA_CATABOLIC_PROCESS, MATTIOLI_MGUS_VS_PCL, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_DN, GOBP_MACROMOLECULE_CATABOLIC_PROCESS, MODULE_308, MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP, GOBP_POST_TRANSCRIPTIONAL_REGULATION_OF_GENE_EXPRESSION, MARTINEZ_RB1_TARGETS_UP, GOBP_REGULATION_OF_CATABOLIC_PROCESS, DOANE_RESPONSE_TO_ANDROGEN_DN, GOBP_REGULATION_OF_CELL_CYCLE, GOBP_MITOCHONDRIAL_RNA_PROCESSING, E4F1_Q6

GO Biological Process (6): mitochondrial RNA processing (GO:0000963), positive regulation of cell population proliferation (GO:0008284), mRNA metabolic process (GO:0016071), regulation of mitochondrial mRNA stability (GO:0044528), regulation of cell cycle (GO:0051726), mitochondrial mRNA processing (GO:0090615)

GO Molecular Function (2): RNA binding (GO:0003723), protein binding (GO:0005515)

GO Cellular Component (3): mitochondrion (GO:0005739), mitochondrial matrix (GO:0005759), ribonucleoprotein granule (GO:0035770)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Mitochondrial RNA degradation	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
mitochondrion	3
mitochondrial RNA metabolic process	2
RNA processing	1
mitochondrial gene expression	1
cell population proliferation	1
regulation of cell population proliferation	1
positive regulation of cellular process	1
RNA metabolic process	1
regulation of mRNA stability	1
cell cycle	1
regulation of cellular process	1
mitochondrial RNA processing	1
mRNA processing	1
nucleic acid binding	1
binding	1
cytoplasm	1
intracellular membrane-bounded organelle	1
intracellular organelle lumen	1
intracellular membraneless organelle	1
supramolecular complex	1

Protein interactions and networks

STRING

1511 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TBRG4	FASTKD5	Q7L8L6	650
TBRG4	SLC49A3	Q6UXD7	589
TBRG4	SUPV3L1	Q8IYB8	576
TBRG4	CCDC201	A0A1B0GTI1	562
TBRG4	KLHL18	O94889	557
TBRG4	RAMP3	O60896	530
TBRG4	DNAJA2	O60884	507
TBRG4	DHX30	Q7L2E3	500
TBRG4	GRSF1	Q12849	483
TBRG4	CCNK	O75909	447
TBRG4	ELAC2	Q9BQ52	443
TBRG4	FASTKD2	Q9NYY8	434
TBRG4	SRFBP1	Q8NEF9	432
TBRG4	S100A1	P23297	427
TBRG4	DDX21	Q9NR30	427

IntAct

227 interactions, top by confidence:

A	B	Type	Score
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
TBRG4	PNPT1	psi-mi:“MI:0914”(association)	0.640
CD27	TCAF2	psi-mi:“MI:0914”(association)	0.640
FAM234B	ABCD4	psi-mi:“MI:0914”(association)	0.620
TBRG4	FAM114A2	psi-mi:“MI:0915”(physical association)	0.600
FAM114A2	TBRG4	psi-mi:“MI:0915”(physical association)	0.600
TBRG4	CALCOCO2	psi-mi:“MI:0915”(physical association)	0.560
TBRG4	AGTRAP	psi-mi:“MI:0915”(physical association)	0.560
FAM9B	TBRG4	psi-mi:“MI:0915”(physical association)	0.560
MAL2	TBRG4	psi-mi:“MI:0915”(physical association)	0.560
CALCOCO2	TBRG4	psi-mi:“MI:0915”(physical association)	0.560
AGTRAP	TBRG4	psi-mi:“MI:0915”(physical association)	0.560
TBRG4	FAM9B	psi-mi:“MI:0915”(physical association)	0.560
TBRG4	MAL2	psi-mi:“MI:0915”(physical association)	0.560
KRT27	TBRG4	psi-mi:“MI:0915”(physical association)	0.560
TMEM239	TBRG4	psi-mi:“MI:0915”(physical association)	0.560
TBRG4	GPRASP2	psi-mi:“MI:0915”(physical association)	0.560
TBRG4	TFIP11	psi-mi:“MI:0915”(physical association)	0.560
CDR2L	TBRG4	psi-mi:“MI:0915”(physical association)	0.560
BICD2	TBRG4	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (446): CALCOCO2 (Two-hybrid), AGTRAP (Two-hybrid), MAL2 (Two-hybrid), FAM9B (Two-hybrid), TBRG4 (Affinity Capture-RNA), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS), TBRG4 (Affinity Capture-MS)

ESM2 similar proteins: A2VD13, A6QP29, A7E3N7, B5DFG1, O75127, P54098, P54099, Q05AA6, Q13474, Q3SX05, Q3SZK4, Q3TYG6, Q3U0L2, Q3U5Q7, Q3URY6, Q3ZBK7, Q4R5Q4, Q53GS7, Q58CQ5, Q5R655, Q5RAS2, Q66H85, Q6NUQ4, Q6ZUX3, Q7YS91, Q80UU1, Q8BKF1, Q8BL74, Q8C0R7, Q8C1F5, Q8C2E4, Q8CJ00, Q8N4P6, Q8R322, Q8TE82, Q969Z0, Q96KN7, Q96MK2, Q99MQ3, Q9BQ95

Diamond homologs: Q3SZK4, Q5M9G9, Q6DJ55, Q6PA48, Q7YS91, Q91YM4, Q969Z0

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

125 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	82
Likely benign	7
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

2217 predictions. Top by Δscore:

Variant	Effect	Δscore
7:45100422:GGGAC:G	acceptor_gain	1.0000
7:45100423:GGAC:G	acceptor_gain	1.0000
7:45100424:GAC:G	acceptor_gain	1.0000
7:45100425:AC:A	acceptor_gain	1.0000
7:45100426:CC:C	acceptor_gain	1.0000
7:45100427:C:CA	acceptor_loss	1.0000
7:45100427:C:CC	acceptor_gain	1.0000
7:45101252:AC:A	donor_loss	1.0000
7:45101253:CTC:C	donor_loss	1.0000
7:45101254:TCA:T	donor_loss	1.0000
7:45101255:CA:C	donor_loss	1.0000
7:45101256:A:AC	donor_gain	1.0000
7:45101256:ACGT:A	donor_gain	1.0000
7:45101256:ACGTC:A	donor_gain	1.0000
7:45101257:C:CA	donor_gain	1.0000
7:45101257:CG:C	donor_gain	1.0000
7:45101257:CGT:C	donor_gain	1.0000
7:45101257:CGTC:C	donor_gain	1.0000
7:45101257:CGTCC:C	donor_gain	1.0000
7:45101259:T:TA	donor_gain	1.0000
7:45101368:CTAGC:C	acceptor_gain	1.0000
7:45101369:TAGC:T	acceptor_gain	1.0000
7:45101370:AGC:A	acceptor_gain	1.0000
7:45101371:GC:G	acceptor_gain	1.0000
7:45101372:CC:C	acceptor_gain	1.0000
7:45101373:C:CC	acceptor_gain	1.0000
7:45101498:CCTA:C	donor_loss	1.0000
7:45101499:CTA:C	donor_loss	1.0000
7:45101500:TACCT:T	donor_loss	1.0000
7:45101502:C:A	donor_loss	1.0000

AlphaMissense

4056 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:45100425:A:T	V599D	0.991
7:45101292:C:G	R587P	0.991
7:45104243:A:C	F307L	0.990
7:45104243:A:T	F307L	0.990
7:45104245:A:G	F307L	0.990
7:45100411:A:G	W604R	0.989
7:45100411:A:T	W604R	0.989
7:45105535:C:G	R214P	0.989
7:45101367:G:T	A562E	0.988
7:45105533:A:G	W215R	0.988
7:45105533:A:T	W215R	0.988
7:45100371:A:G	L617P	0.987
7:45100371:A:T	L617H	0.987
7:45105652:C:G	R175P	0.987
7:45101834:A:G	W520R	0.985
7:45101834:A:T	W520R	0.985
7:45104615:C:G	R277P	0.984
7:45100375:A:C	Y616D	0.983
7:45101271:A:G	F594S	0.982
7:45101313:C:T	G580D	0.981
7:45104618:A:G	L276P	0.981
7:45105451:A:G	L242P	0.981
7:45100361:C:A	K620N	0.980
7:45100361:C:G	K620N	0.980
7:45101612:C:G	A524P	0.980
7:45104597:A:G	L283P	0.980
7:45101314:C:G	G580R	0.978
7:45104709:A:G	C246R	0.978
7:45103366:G:C	F381L	0.977
7:45103366:G:T	F381L	0.977

dbSNP variants (sampled 300 via entrez): RS1000178664 (7:45100719 C>G,T), RS1000194664 (7:45099606 G>A), RS1000204655 (7:45100494 C>T), RS1000253966 (7:45105067 A>G), RS1000539115 (7:45111860 T>A,C), RS1000584871 (7:45103751 G>A), RS1000692262 (7:45106011 A>G), RS1000950876 (7:45103530 C>A,G), RS1001520861 (7:45105646 C>T), RS1001720744 (7:45100049 A>C), RS1002039846 (7:45111459 A>G,T), RS1002066766 (7:45099916 G>A,C), RS1002083237 (7:45100364 G>C,T), RS1002929684 (7:45111867 G>T), RS1003141735 (7:45108922 G>A,C)

Disease associations

OMIM: gene MIM:611325 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST007856_14	Colorectal cancer or advanced adenoma	6.000000e-06
GCST90002400_612	Plateletcrit	4.000000e-09

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0007985	platelet crit

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067265 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.01	Kd	97.07	nM	CHEMBL5653589
7.01	ED50	97.07	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149559: Binding affinity to human TBRG4 incubated for 45 mins by Kinobead based pull down assay	kd	0.0971	uM

CTD chemical–gene interactions

28 total (human), top 28 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects cotreatment, decreases methylation, decreases expression	4
Valproic Acid	decreases expression, increases expression, increases methylation	3
sodium arsenite	decreases expression, increases expression	2
aristolochic acid I	increases expression	1
FR900359	increases phosphorylation	1
beta-lapachone	increases expression	1
4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
pentabromodiphenyl ether	decreases expression	1
corosolic acid	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
ICG 001	decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
bisphenol S	affects expression	1
LDN 193189	affects cotreatment, decreases expression	1
Fulvestrant	decreases methylation, affects cotreatment	1
Acetaminophen	decreases expression	1
Ivermectin	decreases expression	1
Methyl Methanesulfonate	decreases expression	1
Methylnitronitrosoguanidine	increases expression	1
Potassium Dichromate	decreases expression	1
Ribonucleotides	affects binding	1
Smoke	decreases expression	1
Tetrachlorodibenzodioxin	increases expression	1
Thiram	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Tretinoin	decreases expression	1
Chlorodiphenyl (54% Chlorine)	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652601	Binding	Binding affinity to human TBRG4 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

4 cell lines: 3 cancer cell line, 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3J3	Abcam HEK293T TBRG4 KO	Transformed cell line	Female
CVCL_C6Y5	HAP1 FASTKD4-sKO	Cancer cell line	Male
CVCL_C6Y7	HAP1 FASTKD3&4-dKO	Cancer cell line	Male
CVCL_C6Y8	HAP1 FASTKD4&5-dKO	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.