TDRP

Summary

TDRP (testis development related protein, HGNC:26951) is a protein-coding gene on chromosome 8p23.3, encoding Testis development-related protein (Q86YL5). Contributes to normal sperm motility, but not essential for male fertility.

Acts upstream of or within spermatogenesis. Located in cytosol and nucleus.

Source: NCBI Gene 157695 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 53 total — 1 pathogenic
• MANE Select transcript: NM_001384899

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:26951
Approved symbol	TDRP
Name	testis development related protein
Location	8p23.3
Locus type	gene with protein product
Status	Approved
Aliases	INM01, TDRP1, TDRP2
Ensembl gene	ENSG00000180190
Ensembl biotype	protein_coding
OMIM	619049
Entrez	157695

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 2 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000324079, ENST00000523656, ENST00000524229

RefSeq mRNA: 3 — MANE Select: NM_001384899 NM_001256113, NM_001384899, NM_175075

CCDS: CCDS47759, CCDS59090

Canonical transcript exons

ENST00000324079 — 3 exons

Exon	Start	End
ENSE00001226847	489946	492744
ENSE00002114651	544650	544834
ENSE00003593946	494494	494597

Expression profiles

Bgee: expression breadth ubiquitous, 233 present calls, max score 97.75.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 4.2529 / max 77.5792, expressed in 1022 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
91548	4.2529	1022

Top tissues by expression

251 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	97.75	gold quality
oocyte	CL:0000023	96.48	gold quality
right uterine tube	UBERON:0001302	95.38	gold quality
oviduct epithelium	UBERON:0004804	92.94	gold quality
endothelial cell	CL:0000115	92.72	gold quality
right testis	UBERON:0004534	91.95	gold quality
Brodmann (1909) area 23	UBERON:0013554	91.60	gold quality
left testis	UBERON:0004533	91.58	gold quality
epithelial cell of pancreas	CL:0000083	91.56	gold quality
placenta	UBERON:0001987	90.64	gold quality
left lobe of thyroid gland	UBERON:0001120	89.35	gold quality
thyroid gland	UBERON:0002046	89.27	gold quality
testis	UBERON:0000473	89.12	gold quality
middle temporal gyrus	UBERON:0002771	89.05	gold quality
amniotic fluid	UBERON:0000173	89.02	gold quality
right lobe of thyroid gland	UBERON:0001119	89.01	gold quality
germinal epithelium of ovary	UBERON:0001304	88.56	gold quality
pigmented layer of retina	UBERON:0001782	87.88	gold quality
visceral pleura	UBERON:0002401	87.37	gold quality
metanephros cortex	UBERON:0010533	87.29	gold quality
primary visual cortex	UBERON:0002436	86.63	gold quality
caudate nucleus	UBERON:0001873	86.52	gold quality
left ovary	UBERON:0002119	86.43	gold quality
nucleus accumbens	UBERON:0001882	86.35	gold quality
adenohypophysis	UBERON:0002196	86.13	gold quality
fallopian tube	UBERON:0003889	86.06	gold quality
pituitary gland	UBERON:0000007	85.97	gold quality
cortical plate	UBERON:0005343	85.55	gold quality
right ovary	UBERON:0002118	85.21	gold quality
ganglionic eminence	UBERON:0004023	85.17	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-MTAB-3929	yes	95.41
E-MTAB-9388	yes	11.32
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

121 targeting TDRP, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-30A-5P	100.00	76.31	3233
HSA-MIR-30B-5P	100.00	76.29	3248
HSA-MIR-30C-5P	100.00	76.29	3248
HSA-MIR-30D-5P	100.00	76.32	3233
HSA-MIR-30E-5P	100.00	76.32	3242
HSA-MIR-3925-3P	100.00	69.95	1237
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-4482-3P	99.98	72.50	3147
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-3658	99.96	73.87	4379
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753
HSA-MIR-548T-3P	99.96	70.64	3753
HSA-MIR-495-3P	99.96	72.81	4197
HSA-MIR-5688	99.96	73.23	4504
HSA-MIR-545-3P	99.95	70.74	2783
HSA-MIR-128-3P	99.95	71.17	2484
HSA-MIR-216A-3P	99.95	71.19	2505
HSA-MIR-3910	99.95	71.13	2227

Literature-anchored findings (GeneRIF, showing 3)

• These current data demonstrated that as a nuclear factor, TDRP1 might play an important role in spermatogenesis. (PMID:20170638)
• Single nucleotide polymorphisms on genetic loci SOD2, MBP and C8orf42were not found significantly associated with advanced age-related macular degeneration in a Chinese cohort (PMID:24667176)
• TDRP expression in healthy human palatal mucosa is strongly negatively correlated with serum cotinine levels (PMID:31682009)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	si:ch211-225h24.2	ENSDARG00000104936
mus_musculus	Tdrp	ENSMUSG00000050052
rattus_norvegicus	Tdrp	ENSRNOG00000027245

Protein

Protein identifiers

Testis development-related protein — Q86YL5 (reviewed: Q86YL5)

Alternative names: Protein INM01

All UniProt accessions (1): Q86YL5

UniProt curated annotations — full annotation on UniProt →

Function. Contributes to normal sperm motility, but not essential for male fertility.

Subunit / interactions. Interacts with PRM2.

Subcellular location. Nucleus. Cytoplasm.

Tissue specificity. Expressed in spermatogenic cells, especially in spermatocytes (at protein level).

Polymorphism. The poly-Ala region seems to be polymorphic and the number of Ala varies between 4 and 6.

Similarity. Belongs to the TDRP family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q86YL5-1	1, TDRP1	yes
Q86YL5-2	2, TDRP2

RefSeq proteins (3): NP_001243042, NP_001371828, NP_778250 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR031399	TDRP	Family

Pfam: PF15683

UniProt features (4 total): region of interest 2, chain 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q86YL5-F1	60.41	0.01

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 176 (showing top): RORA1_01, GOBP_MALE_GAMETE_GENERATION, HOWLIN_PUBERTAL_MAMMARY_GLAND, GOBP_DEVELOPMENTAL_PROCESS_INVOLVED_IN_REPRODUCTION, CUI_TCF21_TARGETS_2_DN, GAVIN_FOXP3_TARGETS_CLUSTER_P3, ZHOU_INFLAMMATORY_RESPONSE_LIVE_UP, BENPORATH_ES_1, MEISSNER_NPC_HCP_WITH_H3K4ME2_AND_H3K27ME3, RORA2_01, MIKKELSEN_NPC_HCP_WITH_H3K27ME3, LI_INDUCED_T_TO_NATURAL_KILLER_DN, IWANAGA_CARCINOGENESIS_BY_KRAS_PTEN_UP, GSE13522_CTRL_VS_T_CRUZI_BRAZIL_STRAIN_INF_SKIN_DN, GSE13522_CTRL_VS_T_CRUZI_Y_STRAIN_INF_SKIN_IFNAR_KO_UP

GO Biological Process (1): spermatogenesis (GO:0007283)

GO Molecular Function (0):

GO Cellular Component (4): nucleus (GO:0005634), cytoplasm (GO:0005737), mitochondrion (GO:0005739), cytosol (GO:0005829)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
intracellular membrane-bounded organelle	2
cellular anatomical structure	2
cytoplasm	2
developmental process involved in reproduction	1
male gamete generation	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

334 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TDRP	FBXO25	Q8TCJ0	696
TDRP	ZNF596	Q8TC21	645
TDRP	FBXO38	Q6PIJ6	616
TDRP	PRM2	P04554	559
TDRP	ERICH1	Q86X53	547
TDRP	SMOC2	Q9H3U7	534
TDRP	GPCPD1	Q9NPB8	455
TDRP	LINC03040	Q8N319	434
TDRP	KBTBD11	O94819	399
TDRP	CCDC148	Q8NFR7	394
TDRP	EPDR1	Q9UM22	393
TDRP	OR4F21	O95013	380
TDRP	LACC1	Q8IV20	372
TDRP	TRAT1	Q6PIZ9	368
TDRP	ARHGEF10	O15013	368

IntAct

2 interactions, top by confidence:

A	B	Type	Score
CETN1	SFI1	psi-mi:“MI:0914”(association)	0.640

BioGRID (9): TDRP (Affinity Capture-MS), TDRP (Proximity Label-MS), TDRP (Proximity Label-MS), TDRP (Proximity Label-MS), TDRP (Proximity Label-MS), TDRP (Affinity Capture-MS), TDRP (Protein-peptide), TDRP (Affinity Capture-MS), TDRP (Proximity Label-MS)

ESM2 similar proteins: A0A1B0GTI1, A2BIL8, A5PKK9, C5DY61, E2QSX5, E7F555, O35147, O43151, P11805, P19416, P24940, P27579, Q06616, Q17QE3, Q1LZE2, Q1RMQ5, Q1T763, Q28CW2, Q2HR82, Q2TBN9, Q3B8E9, Q3ZBS1, Q567C6, Q5RDK8, Q62417, Q68FW2, Q6AY26, Q6DFB0, Q6P6I6, Q6PKN7, Q80U49, Q86YL5, Q8C3W1, Q8QVM1, Q8VEB3, Q8VI59, Q96FT9, Q96GV9, Q96GY3, Q99618

Diamond homologs: Q498E2, Q5I019, Q86YL5, Q8C5P7

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

53 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	0
Uncertain significance	48
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
1328106	GRCh37/hg19 8p23.3-23.2(chr8:162190-4698813)x1	Pathogenic

SpliceAI

1300 predictions. Top by Δscore:

Variant	Effect	Δscore
8:492745:C:CC	acceptor_gain	1.0000
8:494489:CTTAC:C	donor_loss	1.0000
8:494490:TTACC:T	donor_loss	1.0000
8:494491:TA:T	donor_loss	1.0000
8:494492:A:AC	donor_gain	1.0000
8:494492:AC:A	donor_gain	1.0000
8:494493:C:CC	donor_gain	1.0000
8:494493:CC:C	donor_gain	1.0000
8:494594:GAAC:G	acceptor_gain	1.0000
8:494596:AC:A	acceptor_gain	1.0000
8:494597:CC:C	acceptor_gain	1.0000
8:494598:C:CC	acceptor_gain	1.0000
8:492740:TAGTT:T	acceptor_gain	0.9900
8:492742:GTT:G	acceptor_gain	0.9900
8:492742:GTTC:G	acceptor_loss	0.9900
8:492743:TT:T	acceptor_gain	0.9900
8:492744:TCTA:T	acceptor_loss	0.9900
8:492745:C:CG	acceptor_loss	0.9900
8:492746:T:G	acceptor_loss	0.9900
8:494492:ACC:A	donor_gain	0.9900
8:494493:CCC:C	donor_gain	0.9900
8:494493:CCCT:C	donor_gain	0.9900
8:494593:TGAAC:T	acceptor_gain	0.9900
8:494595:AAC:A	acceptor_gain	0.9900
8:494598:C:T	acceptor_gain	0.9900
8:544145:A:C	donor_gain	0.9900
8:544175:AGTGT:A	donor_gain	0.9900
8:544645:CTCA:C	donor_loss	0.9900
8:544647:CA:C	donor_loss	0.9900
8:544649:C:A	donor_loss	0.9900

AlphaMissense

1191 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
8:492632:A:G	W109R	0.990
8:492632:A:T	W109R	0.990
8:492690:C:A	W89C	0.988
8:492690:C:G	W89C	0.988
8:492692:A:G	W89R	0.988
8:492692:A:T	W89R	0.988
8:492630:C:A	W109C	0.987
8:492630:C:G	W109C	0.987
8:494573:A:G	W45R	0.977
8:494573:A:T	W45R	0.977
8:492693:A:C	F88L	0.975
8:492693:A:T	F88L	0.975
8:492695:A:G	F88L	0.975
8:494571:C:A	W45C	0.975
8:494571:C:G	W45C	0.975
8:492694:A:G	F88S	0.973
8:544754:A:G	W2R	0.973
8:544754:A:T	W2R	0.973
8:544752:C:A	W2C	0.970
8:544752:C:G	W2C	0.970
8:492485:A:G	W158R	0.967
8:492485:A:T	W158R	0.967
8:492635:C:G	G108R	0.951
8:492483:C:A	W158C	0.949
8:492483:C:G	W158C	0.949
8:492631:C:G	W109S	0.949
8:492691:C:G	W89S	0.949
8:494569:T:A	K46I	0.949
8:492672:T:A	K95N	0.946
8:492672:T:G	K95N	0.946

dbSNP variants (sampled 300 via entrez): RS1000007205 (8:512861 A>C), RS1000016748 (8:535845 C>T), RS1000043496 (8:490490 C>T), RS1000072190 (8:490830 T>A,C), RS1000078600 (8:512969 C>T), RS1000084238 (8:532094 G>T), RS1000127570 (8:525876 G>C), RS1000147348 (8:522960 T>C), RS1000196698 (8:497407 G>C), RS1000212146 (8:504238 A>C,G,T), RS1000242690 (8:523093 C>T), RS1000269823 (8:493779 G>A,C), RS1000270565 (8:500849 G>C), RS1000310259 (8:547643 A>G), RS1000326122 (8:543703 T>C)

Disease associations

OMIM: gene MIM:619049 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST001091_1	Bilirubin levels	5.000000e-07
GCST006622_21	Neonatal cytokine/chemokine levels (fetal genetic effect)	2.000000e-09
GCST008513_14	Health literacy	2.000000e-06
GCST008513_15	Health literacy	3.000000e-07

EFO canonical traits (5, from GWAS)

EFO ID	Trait name
EFO:0004570	bilirubin measurement
EFO:0004747	protein measurement
EFO:0007959	fetal genotype effect measurement
EFO:0008184	interleukin 4 measurement
EFO:0010104	health literacy measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

33 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	increases expression, increases methylation, affects cotreatment	7
trichostatin A	affects cotreatment, increases expression	3
sodium arsenite	decreases expression, affects cotreatment, increases abundance, increases expression	3
methylmercuric chloride	decreases expression	2
mercuric bromide	increases expression, affects cotreatment	2
entinostat	increases expression, affects cotreatment	2
Panobinostat	affects cotreatment, increases expression	2
Nickel	decreases expression	2
Phenylmercuric Acetate	affects cotreatment, increases expression	2
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	affects cotreatment, increases methylation	1
manganese chloride	increases abundance, increases expression, affects cotreatment	1
aflatoxin B2	decreases methylation	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	increases expression, affects cotreatment	1
2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazophenyl)amide	decreases reaction, decreases expression	1
dorsomorphin	affects cotreatment, increases expression	1
bisphenol S	affects cotreatment, decreases methylation	1
Temozolomide	affects response to substance	1
Fulvestrant	decreases methylation, affects cotreatment, increases methylation	1
Arsenic	affects cotreatment, increases abundance, increases expression	1
Vehicle Emissions	decreases expression, decreases reaction	1
Benzo(a)pyrene	affects methylation	1
Carmustine	affects response to substance	1
Malathion	decreases expression	1
Manganese	increases abundance, increases expression, affects cotreatment	1
Niclosamide	increases expression	1
Thiram	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Aflatoxin B1	increases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.