TEKT4

Summary

TEKT4 (tektin 4, HGNC:31012) is a protein-coding gene on chromosome 2q11.1, encoding Tektin-4 (Q8WW24). Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme.

Predicted to be involved in cilium assembly and flagellated sperm motility. Predicted to act upstream of with a positive effect on regulation of brood size. Located in axonemal microtubule.

Source: NCBI Gene 150483 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 3 total
• MANE Select transcript: NM_144705

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:31012
Approved symbol	TEKT4
Name	tektin 4
Location	2q11.1
Locus type	gene with protein product
Status	Approved
Aliases	MGC27019
Ensembl gene	ENSG00000163060
Ensembl biotype	protein_coding
Entrez	150483

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 2 protein_coding, 1 retained_intron

ENST00000295201, ENST00000468063, ENST00000941696

RefSeq mRNA: 2 — MANE Select: NM_144705 NM_001286559, NM_144705

CCDS: CCDS2005

Canonical transcript exons

ENST00000295201 — 6 exons

Exon	Start	End
ENSE00001071235	94873520	94873590
ENSE00001839567	94871430	94872077
ENSE00001944857	94876553	94876823
ENSE00003476684	94874776	94874998
ENSE00003492482	94875588	94875742
ENSE00003693453	94873965	94874108

Expression profiles

Bgee: expression breadth ubiquitous, 121 present calls, max score 88.07.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0707 / max 21.5252, expressed in 25 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
21393	0.0470	13
21394	0.0236	9

Top tissues by expression

134 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right uterine tube	UBERON:0001302	88.07	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	79.88	silver quality
left testis	UBERON:0004533	78.41	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	78.01	gold quality
right testis	UBERON:0004534	77.99	gold quality
testis	UBERON:0000473	77.71	gold quality
fallopian tube	UBERON:0003889	69.35	gold quality
apex of heart	UBERON:0002098	67.20	gold quality
lower esophagus mucosa	UBERON:0035834	64.21	gold quality
hypothalamus	UBERON:0001898	60.78	gold quality
right lung	UBERON:0002167	60.56	gold quality
heart left ventricle	UBERON:0002084	59.61	gold quality
caudate nucleus	UBERON:0001873	59.56	gold quality
stromal cell of endometrium	CL:0002255	59.36	gold quality
Ammon’s horn	UBERON:0001954	58.97	gold quality
right adrenal gland	UBERON:0001233	58.83	gold quality
endocervix	UBERON:0000458	58.82	gold quality
right adrenal gland cortex	UBERON:0035827	58.78	gold quality
left uterine tube	UBERON:0001303	58.35	gold quality
mucosa of transverse colon	UBERON:0004991	57.61	gold quality
temporal lobe	UBERON:0001871	57.17	gold quality
amygdala	UBERON:0001876	57.00	gold quality
right lobe of thyroid gland	UBERON:0001119	56.74	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	56.56	gold quality
left adrenal gland	UBERON:0001234	56.39	gold quality
thyroid gland	UBERON:0002046	56.17	gold quality
left adrenal gland cortex	UBERON:0035825	55.85	gold quality
nucleus accumbens	UBERON:0001882	55.84	gold quality
granulocyte	CL:0000094	55.82	gold quality
uterine cervix	UBERON:0000002	55.81	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	8.15

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 4)

• The expression of TEKT4 is significantly decreased in the ejaculated sperm of idiopathic asthenozoospermia patients. (PMID:22774605)
• TEKT4 germline variations in a high cis linkage are biologically relevant, as ectopic expression of variant TEKT4 deregulates microtubule stability, antagonizes paclitaxel-induced stabilizing effect of microtubules and increases paclitaxel resistance. (PMID:24823476)
• Our study revealed that TEKT4 shows important biological implications in papillary thyroid cancer (PMID:30251060)
• Tektin4 loss promotes triple-negative breast cancer metastasis through HDAC6-mediated tubulin deacetylation and increases sensitivity to HDAC6 inhibitor. (PMID:33654196)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	tekt4	ENSDARG00000028899
mus_musculus	Tekt4	ENSMUSG00000024175
rattus_norvegicus	Tekt4	ENSRNOG00000018792
drosophila_melanogaster	Tektin-A	FBGN0028902
caenorhabditis_elegans		WBGENE00019828

Paralogs (5): TEKT2 (ENSG00000092850), TEKT3 (ENSG00000125409), TEKT5 (ENSG00000153060), TEKT1 (ENSG00000167858), (ENSG00000274847)

Protein

Protein identifiers

Tektin-4 — Q8WW24 (reviewed: Q8WW24)

All UniProt accessions (2): A0A384MEB7, Q8WW24

UniProt curated annotations — full annotation on UniProt →

Function. Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules. Contributes to normal sperm motility.

Subunit / interactions. Microtubule inner protein component of sperm flagellar doublet microtubules.

Subcellular location. Cytoplasm. Cytoskeleton. Cilium axoneme. Flagellum axoneme.

Tissue specificity. Strongly expressed in spermatozoa. Also detected at low levels in pancreas. Expressed in airway epithelial cells.

Post-translational modifications. Ubiquitinated, leading to its degradation. Deubiquitinated by USP16, promoting its stability.

Similarity. Belongs to the tektin family.

RefSeq proteins (2): NP_001273488, NP_653306* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000435	Tektins	Family
IPR048256	Tektin-like	Family

Pfam: PF03148

UniProt features (12 total): sequence variant 5, coiled-coil region 3, compositionally biased region 2, chain 1, region of interest 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
7UNG	ELECTRON MICROSCOPY	3.6

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8WW24-F1	91.44	0.82

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 51 (showing top): GOBP_CILIUM_ORGANIZATION, GOBP_CILIUM_MOVEMENT, GOBP_CILIUM_OR_FLAGELLUM_DEPENDENT_CELL_MOTILITY, GOBP_ORGANELLE_ASSEMBLY, GOBP_CELL_PROJECTION_ORGANIZATION, GOCC_CYTOPLASMIC_REGION, GOCC_MOTILE_CILIUM, GOCC_SPERM_PRINCIPAL_PIECE, GOCC_CYTOPLASMIC_MICROTUBULE, GOCC_CILIUM, MATZUK_SPERMATOZOA, chr2q11, MIKKELSEN_MEF_HCP_WITH_H3K27ME3, MARTENS_TRETINOIN_RESPONSE_UP, GOCC_AXONEMAL_MICROTUBULE

GO Biological Process (4): flagellated sperm motility (GO:0030317), cilium assembly (GO:0060271), cilium movement involved in cell motility (GO:0060294), cell projection organization (GO:0030030)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (10): nucleus (GO:0005634), axonemal microtubule (GO:0005879), microtubule cytoskeleton (GO:0015630), sperm flagellum (GO:0036126), axonemal A tubule inner sheath (GO:0160111), cytoplasm (GO:0005737), cytoskeleton (GO:0005856), cilium (GO:0005929), motile cilium (GO:0031514), cell projection (GO:0042995)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
cilium-dependent cell motility	2
cilium movement involved in cell motility	1
sperm motility	1
axoneme assembly	1
intraciliary transport involved in cilium assembly	1
cilium organization	1
protein localization to cilium	1
organelle assembly	1
trans-Golgi to periciliary membrane compartment transport	1
plasma membrane bounded cell projection assembly	1
ciliary transition zone assembly	1
cilium movement	1
cell motility	1
cellular component organization	1
binding	1
intracellular membrane-bounded organelle	1
cytoplasmic microtubule	1
axoneme	1
cytoskeleton	1
9+2 motile cilium	1
A axonemal microtubule	1
axonemal microtubule doublet inner sheath	1
intracellular anatomical structure	1
intracellular membraneless organelle	1
intraciliary transport particle	1
membrane-bounded organelle	1
plasma membrane bounded cell projection	1
cilium	1

Protein interactions and networks

STRING

1261 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TEKT4	ROPN1	Q9HAT0	604
TEKT4	CABYR	O75952	559
TEKT4	TSSK4	Q6SA08	507
TEKT4	SPACA1	Q9HBV2	505
TEKT4	DNAH1	Q9P2D7	505
TEKT4	CCDC39	Q9UFE4	490
TEKT4	DRC5	Q5JU00	478
TEKT4	QRICH1	Q2TAL8	476
TEKT4	ODF2	Q5BJF6	476
TEKT4	MARCHF10	Q8NA82	466
TEKT4	CFAP43	Q8NDM7	465
TEKT4	AKAP4	Q5JQC9	461
TEKT4	CFAP44	Q96MT7	457
TEKT4	SPATA6	Q9NWH7	449
TEKT4	ZPBP	Q9BS86	417

IntAct

374 interactions, top by confidence:

A	B	Type	Score
TEKT4	IKZF3	psi-mi:“MI:0915”(physical association)	0.780
IKZF3	TEKT4	psi-mi:“MI:0915”(physical association)	0.780
MAGEA4	TEKT4	psi-mi:“MI:0915”(physical association)	0.740
RECK	TEKT4	psi-mi:“MI:0915”(physical association)	0.720
TEKT4	ALAS1	psi-mi:“MI:0915”(physical association)	0.720
PRDM14	TEKT4	psi-mi:“MI:0915”(physical association)	0.720
TEKT4	RECK	psi-mi:“MI:0915”(physical association)	0.720
ALAS1	TEKT4	psi-mi:“MI:0915”(physical association)	0.720
TEKT4	PRDM14	psi-mi:“MI:0915”(physical association)	0.720
TEKT4	RARA	psi-mi:“MI:0915”(physical association)	0.670
TEKT4	GSE1	psi-mi:“MI:0915”(physical association)	0.670
TRIP6	TEKT4	psi-mi:“MI:0915”(physical association)	0.670
RARA	TEKT4	psi-mi:“MI:0915”(physical association)	0.670
GSE1	TEKT4	psi-mi:“MI:0915”(physical association)	0.670
TEKT4	TRIP6	psi-mi:“MI:0915”(physical association)	0.670
ATG5	TEKT4	psi-mi:“MI:0915”(physical association)	0.670
TEKT4	ATG5	psi-mi:“MI:0915”(physical association)	0.560
RARA	TEKT4	psi-mi:“MI:0915”(physical association)	0.560
SLC25A6	TEKT4	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (155): TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), TEKT4 (Two-hybrid), KRTAP26-1 (Two-hybrid), VPS33A (Affinity Capture-MS)

ESM2 similar proteins: A1A5Q4, A4IH82, A6H782, A7S8T5, F7F3Q2, G5E8A8, O35594, O46469, Q0E908, Q149S1, Q26648, Q29RL1, Q2T9Q6, Q2TA16, Q2TA38, Q2YDI7, Q32KZ9, Q3SYS9, Q4R353, Q4R5V1, Q4R7G7, Q4V8G8, Q5PPV2, Q5RHQ8, Q5U584, Q5XIJ8, Q6AXV2, Q6AYM2, Q6DFJ6, Q6DGZ3, Q6PE87, Q6X6Z7, Q8CI04, Q8IXS2, Q8IYR0, Q8VHI7, Q8WW24, Q8WYA0, Q922G7, Q95JU3

Diamond homologs: A6H782, F7F3Q2, G5E8A8, P86230, Q149S1, Q1W6C3, Q26648, Q2T9Q6, Q2TA38, Q2YDI7, Q32KZ9, Q4R353, Q4R5V1, Q4V8G8, Q5PPV2, Q6AXV2, Q6AYM2, Q6X6Z7, Q8WW24, Q8WZ33, Q922G7, Q969V4, Q96M29, Q99JD2, Q9BXF9, Q9DAJ2, Q9TTW3, Q9UIF3

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 104 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Keratinization	14	12.8×	6e-10
Formation of the cornified envelope	6	8.6×	6e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

3 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	2
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1339 predictions. Top by Δscore:

Variant	Effect	Δscore
2:94872979:G:GT	donor_gain	1.0000
2:94874104:GAGAG:G	donor_gain	1.0000
2:94874774:A:AG	acceptor_gain	1.0000
2:94874775:G:GA	acceptor_gain	1.0000
2:94874775:GC:G	acceptor_gain	1.0000
2:94874995:CAAGG:C	donor_loss	1.0000
2:94874996:AAGGT:A	donor_loss	1.0000
2:94874997:AGGT:A	donor_loss	1.0000
2:94874998:GG:G	donor_loss	1.0000
2:94875000:T:A	donor_loss	1.0000
2:94872927:A:T	donor_gain	0.9900
2:94872942:G:T	donor_gain	0.9900
2:94872979:G:T	donor_gain	0.9900
2:94873574:GCA:G	donor_gain	0.9900
2:94873577:G:GG	donor_gain	0.9900
2:94873963:A:AG	acceptor_gain	0.9900
2:94873963:AGACT:A	acceptor_gain	0.9900
2:94873964:G:GG	acceptor_gain	0.9900
2:94873964:GACTG:G	acceptor_gain	0.9900
2:94874077:GCTCA:G	donor_gain	0.9900
2:94874106:GAG:G	donor_gain	0.9900
2:94874106:GAGGT:G	donor_loss	0.9900
2:94874107:AGGT:A	donor_loss	0.9900
2:94874108:GG:G	donor_loss	0.9900
2:94874109:GTGG:G	donor_loss	0.9900
2:94874766:C:A	acceptor_gain	0.9900
2:94874773:CAGC:C	acceptor_loss	0.9900
2:94874774:AGC:A	acceptor_gain	0.9900
2:94874775:GCG:G	acceptor_gain	0.9900
2:94874775:GCGC:G	acceptor_gain	0.9900

AlphaMissense

2843 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
2:94872018:C:A	R147S	0.848
2:94875639:G:C	A330P	0.839
2:94874804:T:C	F248L	0.837
2:94874806:C:A	F248L	0.837
2:94874806:C:G	F248L	0.837
2:94875693:C:A	R348S	0.817
2:94876725:C:A	R422S	0.805
2:94876689:A:C	S410R	0.803
2:94876691:T:A	S410R	0.803
2:94876691:T:G	S410R	0.803
2:94872019:G:C	R147P	0.801
2:94873973:G:C	R193P	0.794
2:94871679:T:C	F34L	0.785
2:94871681:C:A	F34L	0.785
2:94871681:C:G	F34L	0.785
2:94875694:G:C	R348P	0.782
2:94873574:G:C	A185P	0.780
2:94876617:G:C	A386P	0.779
2:94871745:T:C	F56L	0.778
2:94871747:C:A	F56L	0.778
2:94871747:C:G	F56L	0.778
2:94872008:C:A	N143K	0.773
2:94872008:C:G	N143K	0.773
2:94875682:G:C	R344P	0.769
2:94872010:T:C	L144P	0.768
2:94875726:G:C	D359H	0.764
2:94876555:T:C	L365P	0.758
2:94871784:A:C	S69R	0.757
2:94871786:C:A	S69R	0.757
2:94871786:C:G	S69R	0.757

dbSNP variants (sampled 300 via entrez): RS10165220 (2:94871255 G>A,T), RS10174668 (2:94871296 C>A,T), RS10180591 (2:94872591 C>T), RS10185133 (2:94877043 G>A,C), RS10191151 (2:94876097 C>A,G,T), RS10191464 (2:94876367 C>A,G,T), RS1052809 (2:94876731 C>T), RS111233845 (2:94875465 G>A), RS111234447 (2:94875725 T>C), RS111254029 (2:94872465 G>A), RS111272026 (2:94871647 C>T), RS111313764 (2:94871125 G>A,T), RS111318238 (2:94873015 A>AGG,AGT), RS111343724 (2:94871624 C>G,T), RS111349391 (2:94871765 G>A,C,T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST012431_2	Parkinson’s disease	8.000000e-10

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

11 total (human), top 11 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	affects methylation, increases expression	2
ferrous chloride	decreases expression	1
Air Pollutants	increases abundance, increases expression	1
Silicon Dioxide	decreases expression	1
Smoke	increases abundance, increases expression	1
Tobacco Smoke Pollution	decreases expression	1
Urethane	increases expression	1
Valproic Acid	increases methylation	1
Cyclosporine	increases expression	1
Aflatoxin B1	decreases methylation	1
Cadmium Chloride	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.