TEKT5

Summary

TEKT5 (tektin 5, HGNC:26554) is a protein-coding gene on chromosome 16p13.13, encoding Tektin-5 (Q96M29). Sperm-specific microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in flagellar axoneme.

Predicted to be involved in cilium assembly and flagellated sperm motility. Located in nucleus.

Source: NCBI Gene 146279 — RefSeq curated summary.

At a glance

• GWAS associations: 12
• Clinical variants (ClinVar): 137 total
• MANE Select transcript: NM_144674

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:26554
Approved symbol	TEKT5
Name	tektin 5
Location	16p13.13
Locus type	gene with protein product
Status	Approved
Aliases	FLJ32871, CT149
Ensembl gene	ENSG00000153060
Ensembl biotype	protein_coding
OMIM	618686
Entrez	146279

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 2 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000283025, ENST00000574923, ENST00000576638

RefSeq mRNA: 1 — MANE Select: NM_144674 NM_144674

CCDS: CCDS10542

Canonical transcript exons

ENST00000283025 — 7 exons

Exon	Start	End
ENSE00001008593	10681993	10682136
ENSE00001008594	10689942	10690025
ENSE00001008596	10675959	10676181
ENSE00001291351	10694310	10694930
ENSE00003537474	10689253	10689323
ENSE00003683209	10627501	10627799
ENSE00003789497	10635764	10635918

Expression profiles

Bgee: expression breadth ubiquitous, 151 present calls, max score 89.83.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0315 / max 34.2825, expressed in 5 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
156214	0.0229	5
156213	0.0086	3

Top tissues by expression

244 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	89.83	gold quality
right testis	UBERON:0004534	89.48	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.89	gold quality
testis	UBERON:0000473	86.43	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	72.30	gold quality
tendon of biceps brachii	UBERON:0008188	63.82	gold quality
mucosa of transverse colon	UBERON:0004991	60.87	gold quality
right lobe of liver	UBERON:0001114	60.47	gold quality
adult organism	UBERON:0007023	59.76	silver quality
cortical plate	UBERON:0005343	58.99	gold quality
right uterine tube	UBERON:0001302	57.47	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	55.01	gold quality
putamen	UBERON:0001874	54.21	gold quality
amygdala	UBERON:0001876	53.62	gold quality
stromal cell of endometrium	CL:0002255	51.80	silver quality
caudate nucleus	UBERON:0001873	51.67	gold quality
Brodmann (1909) area 9	UBERON:0013540	51.35	gold quality
liver	UBERON:0002107	50.31	gold quality
anterior cingulate cortex	UBERON:0009835	50.26	gold quality
ventricular zone	UBERON:0003053	50.13	gold quality
prefrontal cortex	UBERON:0000451	49.77	gold quality
right frontal lobe	UBERON:0002810	49.39	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	49.21	gold quality
nucleus accumbens	UBERON:0001882	49.03	gold quality
oocyte	CL:0000023	48.79	silver quality
cerebellar vermis	UBERON:0004720	48.63	gold quality
right lung	UBERON:0002167	48.41	gold quality
neocortex	UBERON:0001950	48.32	gold quality
Ammon’s horn	UBERON:0001954	47.91	gold quality
temporal lobe	UBERON:0001871	47.88	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.10

Regulation

Is transcription factor: no

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
mus_musculus	Tekt5	ENSMUSG00000039179
rattus_norvegicus	Tekt5	ENSRNOG00000002571
drosophila_melanogaster	CG17450	FBGN0040028
drosophila_melanogaster	CG32819	FBGN0052819
drosophila_melanogaster	CG32820	FBGN0052820

Paralogs (5): TEKT2 (ENSG00000092850), TEKT3 (ENSG00000125409), TEKT4 (ENSG00000163060), TEKT1 (ENSG00000167858), (ENSG00000274847)

Protein

Protein identifiers

Tektin-5 — Q96M29 (reviewed: Q96M29)

All UniProt accessions (2): Q96M29, I3L1F7

UniProt curated annotations — full annotation on UniProt →

Function. Sperm-specific microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in flagellar axoneme. Forms an extensive interaction network in different conformations that reinforces the helix bundle composed by other tektin proteins (TEKT1 to TEKT4) and MIPs to anchor the tektin bundle onto the tubulin wall of A-tubule of the sperm flagellum.

Subunit / interactions. Microtubule inner protein component of sperm flagellar doublet microtubules. Interacts with TEKT3.

Subcellular location. Cytoplasm. Cytoskeleton. Flagellum axoneme.

Post-translational modifications. Ubiquitinated, leading to its degradation. Deubiquitinated by USP16, promoting its stability.

Similarity. Belongs to the tektin family.

RefSeq proteins (1): NP_653275* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000435	Tektins	Family
IPR048256	Tektin-like	Family

Pfam: PF03148

UniProt features (9 total): coiled-coil region 4, sequence variant 4, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96M29-F1	85.96	0.74

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 48 (showing top): CATRRAGC_UNKNOWN, GOBP_CILIUM_ORGANIZATION, GOBP_CILIUM_MOVEMENT, GOBP_CILIUM_OR_FLAGELLUM_DEPENDENT_CELL_MOTILITY, GOBP_ORGANELLE_ASSEMBLY, GOBP_CELL_PROJECTION_ORGANIZATION, GOCC_CYTOPLASMIC_REGION, GOCC_MOTILE_CILIUM, MARSON_BOUND_BY_E2F4_UNSTIMULATED, GOCC_CYTOPLASMIC_MICROTUBULE, GOCC_CILIUM, ZHANG_GATA6_TARGETS_DN, BRUINS_UVC_RESPONSE_VIA_TP53_GROUP_D, GOCC_AXONEMAL_MICROTUBULE, GOCC_9PLUS2_MOTILE_CILIUM

GO Biological Process (3): flagellated sperm motility (GO:0030317), cilium assembly (GO:0060271), cilium movement involved in cell motility (GO:0060294)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (9): nucleus (GO:0005634), microtubule cytoskeleton (GO:0015630), sperm flagellum (GO:0036126), axonemal A tubule inner sheath (GO:0160111), cytoplasm (GO:0005737), cytoskeleton (GO:0005856), cilium (GO:0005929), motile cilium (GO:0031514), cell projection (GO:0042995)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
cilium-dependent cell motility	2
cilium movement involved in cell motility	1
sperm motility	1
axoneme assembly	1
intraciliary transport involved in cilium assembly	1
cilium organization	1
protein localization to cilium	1
organelle assembly	1
trans-Golgi to periciliary membrane compartment transport	1
plasma membrane bounded cell projection assembly	1
ciliary transition zone assembly	1
cilium movement	1
cell motility	1
binding	1
intracellular membrane-bounded organelle	1
cytoskeleton	1
9+2 motile cilium	1
A axonemal microtubule	1
axonemal microtubule doublet inner sheath	1
intracellular anatomical structure	1
intracellular membraneless organelle	1
intraciliary transport particle	1
membrane-bounded organelle	1
plasma membrane bounded cell projection	1
cilium	1

Protein interactions and networks

STRING

1018 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TEKT5	EFHC1	Q5JVL4	451
TEKT5	PARN	O95453	447
TEKT5	MYO15B	Q96JP2	445
TEKT5	DYNLT3	P51808	443
TEKT5	TEX30	Q5JUR7	427
TEKT5	RMI2	Q96E14	426
TEKT5	DRC7	Q8IY82	419
TEKT5	PRPF38B	Q5VTL8	407
TEKT5	DYNLT1	P63172	397
TEKT5	PLA2G10	O15496	389
TEKT5	SPMIP1	A0A1B0GUX0	387
TEKT5	MPV17L	Q2QL34	387
TEKT5	PDXDC1	Q6P996	387
TEKT5	H3BMD7	H3BMD7	381
TEKT5	IZUMO4	Q1ZYL8	379

IntAct

146 interactions, top by confidence:

A	B	Type	Score
TNS2	TEKT5	psi-mi:“MI:0915”(physical association)	0.720
TEKT5	TNS2	psi-mi:“MI:0915”(physical association)	0.720
TEKT5	KLHL38	psi-mi:“MI:0915”(physical association)	0.560
KLHL38	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
TEKT5	RORB	psi-mi:“MI:0915”(physical association)	0.560
HGS	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
USP54	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
PROP1	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
ACTMAP	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
SMUG1	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
KRTAP6-2	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
HOXB6	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
LASP1	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
TEKT5	AKAP8L	psi-mi:“MI:0915”(physical association)	0.560
SPMIP4	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
CSKMT	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
TEKT5	PLSCR4	psi-mi:“MI:0915”(physical association)	0.560
TEKT5	TOX2	psi-mi:“MI:0915”(physical association)	0.560
FOXH1	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
FOXI1	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
KRTAP15-1	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
FNDC3B	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
EYA2	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
KRTAP19-6	TEKT5	psi-mi:“MI:0915”(physical association)	0.560
TEKT5	TBX15	psi-mi:“MI:0915”(physical association)	0.560
KRTAP12-4	TEKT5	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (52): TEKT5 (Two-hybrid), KLHL38 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Affinity Capture-MS), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid), TEKT5 (Two-hybrid)

ESM2 similar proteins: A1A5Q4, A4IH82, A6H782, A7S8T5, F7F3Q2, G5E8A8, O35594, O46469, Q0E908, Q149S1, Q26648, Q29RL1, Q2T9Q6, Q2TA16, Q2TA38, Q2YDI7, Q32KZ9, Q3SYS9, Q4R353, Q4R5V1, Q4R7G7, Q4V8G8, Q5PPV2, Q5RHQ8, Q5U584, Q5XIJ8, Q6AXV2, Q6AYM2, Q6DFJ6, Q6DGZ3, Q6PE87, Q6X6Z7, Q8CI04, Q8IXS2, Q8IYR0, Q8VHI7, Q8WW24, Q8WYA0, Q922G7, Q95JU3

Diamond homologs: A6H782, F7F3Q2, G5E8A8, P86230, Q149S1, Q1W6C3, Q26648, Q2T9Q6, Q2TA38, Q2YDI7, Q32KZ9, Q4R353, Q4R5V1, Q4V8G8, Q5PPV2, Q6AXV2, Q6AYM2, Q6X6Z7, Q8WW24, Q8WZ33, Q922G7, Q969V4, Q96M29, Q99JD2, Q9BXF9, Q9DAJ2, Q9TTW3, Q9UIF3

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 47 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Keratinization	5	11.1×	5e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

137 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	123
Likely benign	5
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1181 predictions. Top by Δscore:

Variant	Effect	Δscore
16:10627797:AGC:A	acceptor_gain	1.0000
16:10627798:GC:G	acceptor_gain	1.0000
16:10627798:GCC:G	acceptor_loss	1.0000
16:10627799:CC:C	acceptor_gain	1.0000
16:10627800:C:T	acceptor_gain	1.0000
16:10627801:T:A	acceptor_loss	1.0000
16:10627805:G:C	acceptor_gain	1.0000
16:10627805:G:GC	acceptor_gain	1.0000
16:10627812:C:CT	acceptor_gain	1.0000
16:10635762:A:AC	donor_gain	1.0000
16:10635763:C:CC	donor_gain	1.0000
16:10635763:CTT:C	donor_gain	1.0000
16:10635916:CGT:C	acceptor_gain	1.0000
16:10635918:TCTG:T	acceptor_loss	1.0000
16:10635919:C:CC	acceptor_gain	1.0000
16:10635919:C:G	acceptor_loss	1.0000
16:10675953:GCTCA:G	donor_loss	1.0000
16:10675954:CTCAC:C	donor_loss	1.0000
16:10675955:TCAC:T	donor_loss	1.0000
16:10675956:CAC:C	donor_loss	1.0000
16:10675957:ACC:A	donor_loss	1.0000
16:10675958:C:T	donor_loss	1.0000
16:10676180:TC:T	acceptor_gain	1.0000
16:10676181:CC:C	acceptor_gain	1.0000
16:10676182:C:CC	acceptor_gain	1.0000
16:10681987:ACTT:A	donor_loss	1.0000
16:10681988:CTT:C	donor_loss	1.0000
16:10681990:T:TG	donor_loss	1.0000
16:10681991:A:AC	donor_gain	1.0000
16:10681991:AC:A	donor_loss	1.0000

AlphaMissense

3208 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
16:10676148:A:C	S299R	0.985
16:10676148:A:T	S299R	0.985
16:10676150:T:G	S299R	0.985
16:10682088:T:A	K256N	0.979
16:10682088:T:G	K256N	0.979
16:10689949:A:G	L214P	0.979
16:10627627:G:T	R472S	0.978
16:10676162:A:G	W295R	0.977
16:10676162:A:T	W295R	0.977
16:10689990:C:A	R200S	0.976
16:10689990:C:G	R200S	0.976
16:10676116:C:G	R310P	0.975
16:10676160:C:A	W295C	0.975
16:10676160:C:G	W295C	0.975
16:10690000:C:G	R197P	0.975
16:10682081:C:G	A259P	0.970
16:10694410:A:G	L155P	0.969
16:10694389:A:G	L162P	0.968
16:10635804:G:T	R401S	0.965
16:10689968:C:G	D208H	0.964
16:10682110:A:G	L249P	0.963
16:10689967:T:G	D208A	0.962
16:10627670:C:A	K457N	0.961
16:10627670:C:G	K457N	0.961
16:10690013:A:G	C193R	0.961
16:10689966:G:C	D208E	0.960
16:10689966:G:T	D208E	0.960
16:10694424:G:C	F150L	0.960
16:10694424:G:T	F150L	0.960
16:10694426:A:G	F150L	0.960

dbSNP variants (sampled 300 via entrez): RS1000028954 (16:10638622 A>G), RS1000092528 (16:10634036 C>A), RS1000122237 (16:10659046 C>A), RS1000165455 (16:10690402 T>C), RS1000192861 (16:10644170 G>A,C), RS1000211870 (16:10662407 C>T), RS1000259086 (16:10679965 A>G), RS1000269158 (16:10651104 C>A,T), RS1000281868 (16:10646667 A>G), RS1000307303 (16:10642111 G>T), RS1000425042 (16:10658800 C>G), RS1000436062 (16:10676427 G>A), RS1000459913 (16:10666973 C>T), RS1000470344 (16:10671519 T>G), RS1000475955 (16:10663811 G>A)

Disease associations

OMIM: gene MIM:618686 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

12 associations (top):

Study	Trait	p-value
GCST001960_7	Eating disorders	5.000000e-06
GCST002928_16	Nickel levels	3.000000e-06
GCST003818_29	Resting heart rate	1.000000e-07
GCST004744_36	Lung adenocarcinoma	6.000000e-07
GCST007001_9	Cerebrospinal AB1-42 levels in normal cognition	5.000000e-07
GCST007430_128	Peak expiratory flow	1.000000e-06
GCST007431_126	Lung function (FEV1/FVC)	2.000000e-31
GCST007448_17	Normal facial asymmetry (angle of surface orientation score)	7.000000e-11
GCST007448_7	Normal facial asymmetry (angle of surface orientation score)	4.000000e-10
GCST007692_73	Chronic obstructive pulmonary disease	5.000000e-08
GCST008481_9	Lung function (FEV1/FVC)	8.000000e-10
GCST010170_4	Neonatal total 25-hydroxyvitamin D levels (maternal genetic effect)	1.000000e-06

EFO canonical traits (5, from GWAS)

EFO ID	Trait name
EFO:0004670	beta-amyloid 1-42 measurement
EFO:0009718	peak expiratory flow
EFO:0004713	FEV/FVC ratio
EFO:0009751	facial asymmetry measurement
EFO:0005939	parental genotype effect measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

11 total (human), top 11 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Aflatoxin B1	decreases methylation, increases methylation	2
fluorene-9-bisphenol	decreases expression	1
terbufos	increases methylation	1
aflatoxin B2	increases methylation	1
bisphenol S	decreases expression	1
Acetaminophen	decreases expression	1
Arsenic	affects methylation	1
Benzo(a)pyrene	decreases methylation	1
Fonofos	increases methylation	1
Parathion	increases methylation	1
Phthalic Acids	increases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.