TEX22

Summary

TEX22 (testis expressed 22, HGNC:40026) is a protein-coding gene on chromosome 14q32.33, encoding Testis-expressed protein 22 (C9J3V5).

Predicted to be located in acrosomal vesicle.

Source: NCBI Gene 647310 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 27 total — 1 pathogenic
• MANE Select transcript: NM_001195082

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:40026
Approved symbol	TEX22
Name	testis expressed 22
Location	14q32.33
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000226174
Ensembl biotype	protein_coding
Entrez	647310

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 4 protein_coding, 1 nonsense_mediated_decay

ENST00000451127, ENST00000548638, ENST00000906980, ENST00000906981, ENST00000935983

RefSeq mRNA: 1 — MANE Select: NM_001195082 NM_001195082

CCDS: CCDS53916

Canonical transcript exons

ENST00000451127 — 4 exons

Exon	Start	End
ENSE00001710967	105411660	105413859
ENSE00001760994	105399302	105399490
ENSE00001782237	105411368	105411496
ENSE00001804724	105398538	105398635

Expression profiles

Bgee: expression breadth ubiquitous, 156 present calls, max score 80.63.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 3.4838 / max 66.3095, expressed in 1348 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
141892	3.4838	1348

Top tissues by expression

242 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	80.63	gold quality
right testis	UBERON:0004534	79.73	gold quality
testis	UBERON:0000473	77.09	gold quality
endothelial cell	CL:0000115	69.98	silver quality
stromal cell of endometrium	CL:0002255	66.50	gold quality
sural nerve	UBERON:0015488	66.22	gold quality
granulocyte	CL:0000094	66.21	gold quality
ventricular zone	UBERON:0003053	65.63	gold quality
bone marrow cell	CL:0002092	65.57	silver quality
right uterine tube	UBERON:0001302	65.20	gold quality
myocardium	UBERON:0002349	64.56	gold quality
secondary oocyte	CL:0000655	64.33	silver quality
colonic epithelium	UBERON:0000397	63.26	gold quality
ganglionic eminence	UBERON:0004023	62.06	gold quality
left ovary	UBERON:0002119	61.94	gold quality
right ovary	UBERON:0002118	61.92	gold quality
right lobe of thyroid gland	UBERON:0001119	61.70	gold quality
right hemisphere of cerebellum	UBERON:0014890	61.67	gold quality
bone marrow	UBERON:0002371	61.40	gold quality
cerebellar hemisphere	UBERON:0002245	61.08	gold quality
vermiform appendix	UBERON:0001154	61.02	gold quality
cerebellar cortex	UBERON:0002129	60.73	gold quality
blood	UBERON:0000178	60.66	gold quality
spleen	UBERON:0002106	60.51	gold quality
mucosa of transverse colon	UBERON:0004991	60.29	gold quality
lymph node	UBERON:0000029	60.28	gold quality
cerebellum	UBERON:0002037	60.15	gold quality
body of uterus	UBERON:0009853	59.99	gold quality
left lobe of thyroid gland	UBERON:0001120	59.81	gold quality
metanephros	UBERON:0000081	59.62	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.76

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

36 targeting TEX22, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-6756-5P	99.82	67.97	2466
HSA-MIR-6766-5P	99.68	67.70	2325
HSA-MIR-7-5P	99.67	70.53	1809
HSA-MIR-18A-3P	99.56	65.68	1092
HSA-MIR-12122	99.56	69.33	1672
HSA-MIR-486-3P	99.51	66.82	1901
HSA-MIR-6727-3P	99.49	65.92	1333
HSA-MIR-6081	99.48	66.07	1446
HSA-MIR-4722-3P	99.35	65.22	1099
HSA-MIR-548V	99.29	69.47	1157
HSA-MIR-6731-5P	99.28	67.42	2375
HSA-MIR-8085	99.28	67.56	2362
HSA-MIR-3688-5P	99.12	69.67	1091
HSA-MIR-4324	99.04	70.14	1569
HSA-MIR-7151-3P	99.04	69.72	2370
HSA-MIR-3619-5P	99.00	68.87	2308
HSA-MIR-2467-3P	98.65	67.18	1969
HSA-MIR-7977	98.65	66.18	2590
HSA-MIR-210-5P	98.57	64.37	832
HSA-MIR-4704-3P	98.28	69.33	1300
HSA-MIR-338-3P	98.14	67.38	1137
HSA-MIR-6847-5P	97.93	66.74	1808
HSA-MIR-924	97.78	66.21	681
HSA-MIR-296-5P	97.61	64.02	851
HSA-MIR-1226-3P	97.51	66.32	1063
HSA-MIR-3620-5P	97.42	63.95	792
HSA-MIR-6859-3P	97.26	64.69	428
HSA-MIR-4286	97.20	64.37	1587
HSA-MIR-600	97.07	66.73	1259

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Tex22	ENSMUSG00000012211
rattus_norvegicus	Tex22	ENSRNOG00000028158

Protein

Protein identifiers

Testis-expressed protein 22 — C9J3V5 (reviewed: C9J3V5)

All UniProt accessions (2): C9J3V5, F8VX47

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Cytoplasm. Cytoplasmic vesicle. Secretory vesicle. Acrosome.

RefSeq proteins (1): NP_001182011* (*=MANE)

Domains & families (InterPro)

UniProt features (3 total): chain 1, region of interest 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-C9J3V5-F1	61.58	0.03

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 27 (showing top): GOCC_SECRETORY_GRANULE, chr14q32, GOCC_SECRETORY_VESICLE, GOCC_ACROSOMAL_VESICLE, BAKKER_FOXO3_TARGETS_DN, ZBTB12_TARGET_GENES, ZNF592_TARGET_GENES, GSE11864_UNTREATED_VS_CSF1_IFNG_IN_MAC_UP, GSE13485_DAY1_VS_DAY7_YF17D_VACCINE_PBMC_UP, GSE13485_DAY3_VS_DAY7_YF17D_VACCINE_PBMC_UP, DESCARTES_FETAL_PLACENTA_MEGAKARYOCYTES, FXR1_TARGET_GENES, ZNF320_TARGET_GENES, GSE30083_SP1_VS_SP3_THYMOCYTE_UP, GSE32423_CTRL_VS_IL7_MEMORY_CD8_TCELL_DN

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (3): acrosomal vesicle (GO:0001669), cytoplasm (GO:0005737), cytoplasmic vesicle (GO:0031410)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
secretory granule	1
intracellular anatomical structure	1
cellular anatomical structure	1
cytoplasm	1
intracellular vesicle	1

Protein interactions and networks

STRING

178 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TEX22	TEX26	Q8N6G2	507
TEX22	BTBD6	Q96KE9	507
TEX22	NUDT14	O95848	507
TEX22	OR8H3	Q8N146	506
TEX22	TEX2	Q8IWB9	504
TEX22	TEX13C	A0A0J9YWL9	480
TEX22	PACS2	Q86VP3	474
TEX22	TEX11	Q8IYF3	440
TEX22	RAB36	O95755	439
TEX22	TEX19	Q8NA77	433
TEX22	TEX10	Q9NXF1	426
TEX22	TEX9	Q8N6V9	418
TEX22	PLD4	Q96BZ4	404
TEX22	FAM72B	Q86X60	399
TEX22	TMEM121	Q9BTD3	398

IntAct

0 interactions, top by confidence:

BioGRID (3): TEX22 (Affinity Capture-RNA), TEX22 (Positive Genetic), TEX22 (Affinity Capture-RNA)

ESM2 similar proteins: A0A023PYD3, A0A179HM40, A4VS83, A5LFY3, A5U9Q0, A6X0W8, A8MUN3, A9G6D9, B0BND4, B1AUF7, C1AJ62, C1B7S4, C1DNF8, C9J3V5, P0C5R2, P0DRI5, P16759, P17145, P23788, P25246, P32757, P64900, P67301, P9WFL8, P9WFL9, P9WLR2, P9WLR3, Q05102, Q08419, Q09215, Q0D252, Q0SAH0, Q1IV77, Q21QM3, Q28470, Q2VZ16, Q48BF1, Q49KT9, Q4G0N7, Q4JSC3

Diamond homologs: C9J3V5, Q9D9U4

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

27 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	0
Uncertain significance	21
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
2685500	GRCh37/hg19 14q32.2-32.33(chr14:101024609-107285437)x1	Pathogenic

SpliceAI

1760 predictions. Top by Δscore:

Variant	Effect	Δscore
14:105438663:T:A	acceptor_gain	1.0000
14:105445413:TACAG:T	acceptor_loss	1.0000
14:105445415:CAGAC:C	acceptor_loss	1.0000
14:105445508:GCAA:G	donor_gain	1.0000
14:105445511:AGTG:A	donor_loss	1.0000
14:105445512:G:GG	donor_gain	1.0000
14:105445513:T:G	donor_loss	1.0000
14:105450054:TCA:T	acceptor_loss	1.0000
14:105450055:CAG:C	acceptor_loss	1.0000
14:105450056:A:AG	acceptor_gain	1.0000
14:105450056:AG:A	acceptor_gain	1.0000
14:105450056:AGG:A	acceptor_gain	1.0000
14:105450056:AGGG:A	acceptor_gain	1.0000
14:105450057:G:GA	acceptor_gain	1.0000
14:105450057:GG:G	acceptor_gain	1.0000
14:105450057:GGG:G	acceptor_gain	1.0000
14:105450057:GGGG:G	acceptor_gain	1.0000
14:105450057:GGGGA:G	acceptor_gain	1.0000
14:105420058:TCGG:T	donor_gain	0.9900
14:105438667:TGCAG:T	acceptor_loss	0.9900
14:105438669:CAGAC:C	acceptor_loss	0.9900
14:105438670:A:AG	acceptor_gain	0.9900
14:105438671:G:GC	acceptor_gain	0.9900
14:105438671:GA:G	acceptor_gain	0.9900
14:105438671:GAC:G	acceptor_gain	0.9900
14:105438671:GACT:G	acceptor_gain	0.9900
14:105438737:AAG:A	donor_loss	0.9900
14:105438739:GGTAC:G	donor_loss	0.9900
14:105438741:T:A	donor_loss	0.9900
14:105445416:A:AG	acceptor_gain	0.9900

AlphaMissense

965 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
14:105399490:G:C	W50C	0.970
14:105399490:G:T	W50C	0.970
14:105411407:T:A	W64R	0.963
14:105411407:T:C	W64R	0.963
14:105411416:A:C	S67R	0.963
14:105411418:C:A	S67R	0.963
14:105411418:C:G	S67R	0.963
14:105411765:T:C	F129L	0.959
14:105411767:C:A	F129L	0.959
14:105411767:C:G	F129L	0.959
14:105411428:C:A	R71S	0.956
14:105411409:G:C	W64C	0.951
14:105411409:G:T	W64C	0.951
14:105399488:T:A	W50R	0.948
14:105399488:T:C	W50R	0.948
14:105411756:T:C	F126L	0.948
14:105411758:C:A	F126L	0.948
14:105411758:C:G	F126L	0.948
14:105411417:G:T	S67I	0.933
14:105411708:G:C	D110H	0.924
14:105411685:T:C	L102P	0.923
14:105411432:G:C	R72P	0.922
14:105399485:G:C	D49H	0.909
14:105411429:G:C	R71P	0.894
14:105399486:A:T	D49V	0.892
14:105411710:C:A	D110E	0.892
14:105411710:C:G	D110E	0.892
14:105411766:T:C	F129S	0.885
14:105411707:G:C	K109N	0.881
14:105411707:G:T	K109N	0.881

dbSNP variants (sampled 300 via entrez): RS1000879303 (14:105396807 C>T), RS1000908816 (14:105396561 C>T), RS1001029210 (14:105405208 T>G), RS1001290981 (14:105401560 C>T), RS1001727493 (14:105402145 C>A,T), RS1001759610 (14:105397640 G>A), RS1002324449 (14:105400994 G>T), RS1002504804 (14:105403436 G>A), RS1002606045 (14:105399772 C>T), RS1002703888 (14:105409309 G>A), RS1002766815 (14:105403206 A>T), RS1002770234 (14:105410748 G>A,C,T), RS1003191547 (14:105413938 G>A,C), RS1004056448 (14:105411728 G>A,T), RS1004870987 (14:105397351 G>A)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

11 total (human), top 11 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects cotreatment, decreases methylation	1
ferrous chloride	decreases expression	1
aflatoxin B2	decreases methylation	1
abrine	increases expression	1
jinfukang	affects cotreatment, decreases expression	1
Fulvestrant	affects cotreatment, decreases methylation	1
Cisplatin	affects cotreatment, decreases expression	1
Aflatoxin B1	decreases methylation	1
Okadaic Acid	decreases expression	1
S-Nitrosoglutathione	decreases expression	1
Magnetite Nanoparticles	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.