TEX35

Summary

TEX35 (testis expressed 35, HGNC:25366) is a protein-coding gene on chromosome 1q25.2, encoding Testis-expressed protein 35 (Q5T0J7).

Located in microtubule cytoskeleton.

Source: NCBI Gene 84066 — RefSeq curated summary.

At a glance

• GWAS associations: 5
• Clinical variants (ClinVar): 32 total
• MANE Select transcript: NM_032126

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:25366
Approved symbol	TEX35
Name	testis expressed 35
Location	1q25.2
Locus type	gene with protein product
Status	Approved
Aliases	DKFZP564J047, TSC24
Ensembl gene	ENSG00000240021
Ensembl biotype	protein_coding
Entrez	84066

Gene structure

Transcript identifiers

Ensembl transcripts: 7 — 6 protein_coding, 1 nonsense_mediated_decay

ENST00000319416, ENST00000367639, ENST00000367641, ENST00000367642, ENST00000367643, ENST00000419909, ENST00000442872

RefSeq mRNA: 4 — MANE Select: NM_032126 NM_001170722, NM_001170723, NM_001170724, NM_032126

CCDS: CCDS1323, CCDS53433, CCDS53434

Canonical transcript exons

ENST00000319416 — 9 exons

Exon	Start	End
ENSE00000921998	178522325	178522650
ENSE00003533827	178514700	178514768
ENSE00003542904	178521222	178521264
ENSE00003562987	178520673	178520874
ENSE00003567891	178516615	178516674
ENSE00003585639	178514027	178514077
ENSE00003587246	178520372	178520436
ENSE00003634397	178515859	178515915
ENSE00003674315	178513109	178513227

Expression profiles

Bgee: expression breadth ubiquitous, 113 present calls, max score 96.59.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.1446 / max 149.7602, expressed in 3 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
6877	0.1287	3
6878	0.0159	3

Top tissues by expression

214 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	96.59	gold quality
right testis	UBERON:0004534	96.38	gold quality
testis	UBERON:0000473	93.01	gold quality
sperm	CL:0000019	87.26	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	78.25	gold quality
kidney epithelium	UBERON:0004819	77.32	gold quality
pancreatic ductal cell	CL:0002079	77.30	silver quality
cardiac muscle of right atrium	UBERON:0003379	76.28	gold quality
upper arm skin	UBERON:0004263	75.78	gold quality
left ventricle myocardium	UBERON:0006566	75.73	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	75.20	gold quality
myocardium	UBERON:0002349	74.52	gold quality
buccal mucosa cell	CL:0002336	69.67	gold quality
vena cava	UBERON:0004087	69.60	gold quality
vastus lateralis	UBERON:0001379	69.54	gold quality
quadriceps femoris	UBERON:0001377	69.34	gold quality
nasal cavity epithelium	UBERON:0005384	68.91	gold quality
epithelial cell of pancreas	CL:0000083	68.37	gold quality
adult organism	UBERON:0007023	67.20	gold quality
pons	UBERON:0000988	66.67	silver quality
superficial temporal artery	UBERON:0001614	65.70	gold quality
body of tongue	UBERON:0011876	65.05	gold quality
subthalamic nucleus	UBERON:0001906	64.80	gold quality
gingival epithelium	UBERON:0001949	64.74	gold quality
pericardium	UBERON:0002407	64.73	silver quality
dorsal plus ventral thalamus	UBERON:0001897	64.50	gold quality
nipple	UBERON:0002030	64.32	gold quality
tongue	UBERON:0001723	64.14	gold quality
esophagus squamous epithelium	UBERON:0006920	63.97	gold quality
substantia nigra pars compacta	UBERON:0001965	63.88	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-GEOD-111727	yes	78.90
E-ANND-3	yes	3.93

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

16 targeting TEX35, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-7978	99.86	66.90	856
HSA-MIR-205-5P	99.81	70.05	1557
HSA-MIR-498-5P	99.76	69.64	1807
HSA-MIR-143-3P	99.49	69.05	1457
HSA-MIR-4770	99.49	69.09	1451
HSA-MIR-766-5P	99.47	67.91	2225
HSA-MIR-4786-3P	99.36	68.35	1390
HSA-MIR-6088	99.29	68.45	1284
HSA-MIR-4650-3P	99.01	68.39	1062
HSA-MIR-4451	98.82	68.17	1455
HSA-MIR-548S	98.50	67.17	1213
HSA-MIR-7843-3P	98.31	67.94	803
HSA-MIR-5691	98.23	67.02	1335
HSA-MIR-6805-3P	98.23	67.02	1334

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Tex35	ENSMUSG00000026592
rattus_norvegicus	Tex35	ENSRNOG00000038921

Protein

Protein identifiers

Testis-expressed protein 35 — Q5T0J7 (reviewed: Q5T0J7)

All UniProt accessions (4): Q5T0J7, E5RIH0, Q5T0J5, Q5T0K0

UniProt curated annotations — full annotation on UniProt →

Tissue specificity. Testis-specific.

Isoforms (5)

UniProt ID	Names	Canonical?
Q5T0J7-1	1	yes
Q5T0J7-2	2
Q5T0J7-3	3
Q5T0J7-4	4
Q5T0J7-5	5

RefSeq proteins (4): NP_001164193, NP_001164194, NP_001164195, NP_115502* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR027874	Tex35	Family

Pfam: PF15079

UniProt features (14 total): splice variant 6, sequence variant 4, chain 1, region of interest 1, sequence conflict 1, coiled-coil region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q5T0J7-F1	64.63	0.27

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 41 (showing top): AP4_Q6, TGACCTY_ERR1_Q2, CAGCTG_AP4_Q5, ER_Q6_02, chr1q25, ER_Q6_01, PR_01, BRUINS_UVC_RESPONSE_VIA_TP53_GROUP_C, ZNF618_TARGET_GENES, GSE10239_NAIVE_VS_MEMORY_CD8_TCELL_DN, MIR4786_3P, MIR548S, MIR4326, MIR7843_3P, MIR7156_3P

GO Biological Process (0):

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (2): nucleus (GO:0005634), microtubule cytoskeleton (GO:0015630)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
binding	1
intracellular membrane-bounded organelle	1
cytoskeleton	1

Protein interactions and networks

STRING

302 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TEX35	RALGPS2	Q86X27	953
TEX35	RGSL1	A5PLK6	896
TEX35	GRIP1	Q9Y3R0	729
TEX35	SPATA45	Q537H7	664
TEX35	SPMIP10	Q6ZNM6	657
TEX35	SPMIP9	Q96LM6	571
TEX35	SPEM1	Q8N4L4	518
TEX35	CBY2	Q8NA61	498
TEX35	SPATC1	Q76KD6	485
TEX35	SPATA3	Q8NHX4	482
TEX35	SPMIP3	Q5SVJ3	479
TEX35	SPATA25	Q9BR10	464
TEX35	FHIP2A	Q5W0V3	459
TEX35	SPATA19	Q7Z5L4	437
TEX35	FOCAD	Q5VW36	379

IntAct

9 interactions, top by confidence:

A	B	Type	Score
BRAP	TEX35	psi-mi:“MI:0915”(physical association)	0.370
TEX35	SUV39H1	psi-mi:“MI:0915”(physical association)	0.370
KDM1A	TEX35	psi-mi:“MI:0915”(physical association)	0.370
PRMT6	TEX35	psi-mi:“MI:0915”(physical association)	0.370
CUL5	DDX3X	psi-mi:“MI:0914”(association)	0.350
TEX35	SNAPIN	psi-mi:“MI:0914”(association)	0.350

BioGRID (98): GOLGA2 (Affinity Capture-MS), HEPHL1 (Affinity Capture-MS), GOLGB1 (Affinity Capture-MS), BIRC6 (Affinity Capture-MS), GORASP1 (Affinity Capture-MS), CALML3 (Affinity Capture-MS), BLOC1S2 (Affinity Capture-MS), LYG2 (Affinity Capture-MS), BLOC1S1 (Affinity Capture-MS), S100A3 (Affinity Capture-MS), PLCD1 (Affinity Capture-MS), RABGAP1 (Affinity Capture-MS), LOH12CR1 (Affinity Capture-MS), DTNBP1 (Affinity Capture-MS), BLOC1S5 (Affinity Capture-MS)

ESM2 similar proteins: A0A1B0GTR0, A5WWB6, A6H7E2, D3Z0R2, F4KIH4, F5HGK9, O36397, O55527, O55779, O74982, O93036, P04493, P04861, P04862, P05857, P05904, P14253, P14254, P14355, P18801, P21280, P27194, P34431, P34743, P35940, P52546, P68966, P68967, P69738, Q01226, Q02484, Q0A2C9, Q0A2H8, Q148F6, Q14BK3, Q1X700, Q2TBV0, Q2YDP6, Q32L75, Q3TTJ4

Diamond homologs: Q14BK3, Q2YDP6, Q5T0J7

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

32 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	29
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1112 predictions. Top by Δscore:

Variant	Effect	Δscore
1:178514025:A:AG	acceptor_gain	1.0000
1:178514026:G:GG	acceptor_gain	1.0000
1:178514026:GA:G	acceptor_gain	1.0000
1:178514074:CAAAG:C	donor_loss	1.0000
1:178514075:AAAGT:A	donor_loss	1.0000
1:178514076:AAGT:A	donor_loss	1.0000
1:178514077:AG:A	donor_loss	1.0000
1:178514078:G:GG	donor_gain	1.0000
1:178514078:GTA:G	donor_loss	1.0000
1:178514079:T:G	donor_loss	1.0000
1:178515849:A:AG	acceptor_gain	1.0000
1:178515857:A:AG	acceptor_gain	1.0000
1:178515858:G:GG	acceptor_gain	1.0000
1:178515913:CAGGT:C	donor_loss	1.0000
1:178515914:AGGT:A	donor_loss	1.0000
1:178515915:GGTA:G	donor_loss	1.0000
1:178515916:G:C	donor_loss	1.0000
1:178515917:T:G	donor_loss	1.0000
1:178514021:TTGCA:T	acceptor_loss	0.9900
1:178514022:TGCA:T	acceptor_loss	0.9900
1:178514023:GCAGA:G	acceptor_loss	0.9900
1:178514024:CAG:C	acceptor_gain	0.9900
1:178514025:AGA:A	acceptor_gain	0.9900
1:178514026:GAG:G	acceptor_gain	0.9900
1:178514026:GAGCA:G	acceptor_gain	0.9900
1:178514076:AA:A	donor_gain	0.9900
1:178514129:G:GT	donor_gain	0.9900
1:178514129:G:T	donor_gain	0.9900
1:178514691:A:AG	acceptor_gain	0.9900
1:178514695:CACAG:C	acceptor_loss	0.9900

AlphaMissense

1548 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:178515887:T:C	L63P	0.995
1:178516639:T:C	F81L	0.992
1:178516641:T:A	F81L	0.992
1:178516641:T:G	F81L	0.992
1:178516649:T:C	L84P	0.990
1:178520380:G:C	Q95H	0.990
1:178520380:G:T	Q95H	0.990
1:178516658:T:C	F87S	0.985
1:178516620:G:C	K74N	0.982
1:178516620:G:T	K74N	0.982
1:178516657:T:C	F87L	0.982
1:178516659:T:A	F87L	0.982
1:178516659:T:G	F87L	0.982
1:178520379:A:C	Q95P	0.982
1:178516649:T:A	L84H	0.980
1:178520409:T:C	L105S	0.980
1:178516658:T:G	F87C	0.977
1:178516671:G:A	M91I	0.977
1:178516671:G:C	M91I	0.977
1:178516671:G:T	M91I	0.977
1:178515866:T:C	L56P	0.975
1:178515887:T:A	L63H	0.975
1:178516637:A:C	D80A	0.973
1:178520376:T:C	M94T	0.972
1:178515896:A:T	K66I	0.969
1:178516670:T:G	M91R	0.969
1:178515908:T:A	I70K	0.968
1:178516616:T:C	I73T	0.968
1:178516636:G:C	D80H	0.968
1:178516637:A:T	D80V	0.968

dbSNP variants (sampled 300 via entrez): RS1000231806 (1:178518916 T>C), RS1000271538 (1:178520680 A>C,T), RS1000281580 (1:178520850 C>T), RS1000708178 (1:178518720 A>G), RS1001302625 (1:178511540 T>A,G), RS1001303192 (1:178517227 A>G), RS1001442730 (1:178517944 A>G), RS1001514093 (1:178515375 A>G), RS1001630156 (1:178517543 C>T), RS1002072498 (1:178521345 C>A,T), RS1002633429 (1:178516460 A>G), RS1003142159 (1:178522784 A>G), RS1003241082 (1:178511112 G>A), RS1003480939 (1:178523065 T>G), RS1003636762 (1:178517909 T>A,C)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

5 associations (top):

Study	Trait	p-value
GCST001762_167	Obesity-related traits	6.000000e-06
GCST010241_51	Apolipoprotein A1 levels	6.000000e-15
GCST010242_430	HDL cholesterol levels	2.000000e-21
GCST010244_82	Triglyceride levels	2.000000e-09
GCST90002404_438	Red cell distribution width	2.000000e-11

EFO canonical traits (4, from GWAS)

EFO ID	Trait name
EFO:0004614	apolipoprotein A 1 measurement
EFO:0004612	high density lipoprotein cholesterol measurement
EFO:0004530	triglyceride measurement
EFO:0009188	Red cell distribution width

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

5 total (human), top 5 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
propionaldehyde	decreases expression	1
Acetaminophen	increases expression	1
Cyclosporine	decreases methylation	1
Aflatoxin B1	increases methylation	1
Copper Sulfate	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.