TEX50

Summary

TEX50 (testis expressed 50, HGNC:52382) is a protein-coding gene on chromosome 1q25.1, encoding Testis-expressed protein 50 (A0A1B0GTY4). Sperm membrane protein required for stability of the sperm acrosome after its formation in the testis.

Predicted to be located in membrane.

Source: NCBI Gene 730159 — RefSeq curated summary.

At a glance

• MANE Select transcript: NM_001195190

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:52382
Approved symbol	TEX50
Name	testis expressed 50
Location	1q25.1
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000232113
Ensembl biotype	protein_coding
OMIM	621346
Entrez	730159

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000417563

RefSeq mRNA: 1 — MANE Select: NM_001195190 NM_001195190

CCDS: CCDS81405

Canonical transcript exons

ENST00000417563 — 2 exons

Exon	Start	End
ENSE00001740983	173635338	173635845
ENSE00001781248	173636827	173637130

Expression profiles

Bgee: expression breadth tissue_specific, 9 present calls, max score 91.89.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0109 / max 9.9239, expressed in 3 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
6731	0.0063	3
6732	0.0046	3

Top tissues by expression

123 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	91.89	gold quality
right testis	UBERON:0004534	86.05	gold quality
left testis	UBERON:0004533	85.20	gold quality
testis	UBERON:0000473	84.75	gold quality
cerebellar vermis	UBERON:0004720	76.33	gold quality
thymus	UBERON:0002370	58.17	silver quality
corpus callosum	UBERON:0002336	43.04	gold quality
granulocyte	CL:0000094	38.92	gold quality
colonic epithelium	UBERON:0000397	37.20	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
bone marrow cell	CL:0002092	36.16	gold quality
ganglionic eminence	UBERON:0004023	35.49	gold quality
mucosa of stomach	UBERON:0001199	35.19	gold quality
skeletal muscle tissue	UBERON:0001134	33.66	gold quality
hindlimb stylopod muscle	UBERON:0004252	32.15	gold quality
bone marrow	UBERON:0002371	32.00	gold quality
muscle tissue	UBERON:0002385	31.29	gold quality
sural nerve	UBERON:0015488	30.93	gold quality
liver	UBERON:0002107	29.94	gold quality
stromal cell of endometrium	CL:0002255	29.87	gold quality
prefrontal cortex	UBERON:0000451	29.04	gold quality
leukocyte	CL:0000738	28.71	gold quality
duodenum	UBERON:0002114	28.14	gold quality
monocyte	CL:0000576	28.12	silver quality
olfactory segment of nasal mucosa	UBERON:0005386	27.80	silver quality
lymph node	UBERON:0000029	27.57	gold quality
tonsil	UBERON:0002372	27.05	gold quality
islet of Langerhans	UBERON:0000006	26.55	gold quality
blood	UBERON:0000178	26.48	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.14

Regulation

Is transcription factor: no

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Tex50	ENSMUSG00000049160
rattus_norvegicus	Tex50	ENSRNOG00000038786

Protein

Protein identifiers

Testis-expressed protein 50 — A0A1B0GTY4 (reviewed: A0A1B0GTY4)

All UniProt accessions (1): A0A1B0GTY4

UniProt curated annotations — full annotation on UniProt →

Function. Sperm membrane protein required for stability of the sperm acrosome after its formation in the testis. Essential for male fertility.

Subcellular location. Membrane.

Tissue specificity. Testis-specific.

RefSeq proteins (1): NP_001182119* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR038833	TEX50	Family

UniProt features (5 total): signal peptide 1, chain 1, transmembrane region 1, region of interest 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A0A1B0GTY4-F1	65.84	0.13

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 2 (showing top): chr1q25, MAFG_TARGET_GENES

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (1): membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	1

Protein interactions and networks

STRING

36 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TEX50	ZBTB37	Q5TC79	643
TEX50	ANKRD45	Q5TZF3	633
TEX50	KIAA0040	Q15053	585
TEX50	SLC9C2	Q5TAH2	561
TEX50	PIGC	Q92535	476
TEX50	KLHL20	Q9Y2M5	443
TEX50	DARS2	Q6PI48	435
TEX50	CENPL	Q8N0S6	419
TEX50	SUCO	Q9UBS9	408
TEX50	CACYBP	Q9HB71	392
TEX50	RC3H1	Q5TC82	391
TEX50	DNM3	Q9UQ16	337
TEX50	TNFSF18	Q9UNG2	310
TEX50	PRDX6	P30041	270
TEX50	TNFSF4	P23510	229

IntAct

0 interactions, top by confidence:

ESM2 similar proteins: A0A1B0GTR0, A0A1B0GTY4, A0A1B0GVD1, A7WNB0, A8R0V4, E9Q7F5, O55779, O70552, O75818, O93036, P01586, P03212, P03319, P03321, P04823, P0DOE0, P10260, P13206, P16837, P20880, P28907, P36340, P47939, P47940, Q02484, Q03233, Q1HVF6, Q2HRD2, Q3KSS3, Q3TTJ4, Q5BK38, Q5QR91, Q5VAN0, Q64277, Q66669, Q66672, Q6GQU0, Q6GVM5, Q6UWF9, Q86WR6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

118 predictions. Top by Δscore:

Variant	Effect	Δscore
1:173635537:C:CT	donor_gain	0.9500
1:173635538:T:TT	donor_gain	0.9500
1:173635536:A:AC	donor_gain	0.8900
1:173635565:T:C	donor_gain	0.7900
1:173635540:CCG:C	donor_gain	0.7500
1:173635541:CGC:C	donor_gain	0.7100
1:173636104:C:CT	donor_gain	0.7100
1:173636105:T:TT	donor_gain	0.7100
1:173635679:ACTG:A	donor_gain	0.6100
1:173635680:CTGC:C	donor_gain	0.6100
1:173635767:A:AC	acceptor_gain	0.6100
1:173635692:T:C	donor_gain	0.5700
1:173636138:T:C	donor_gain	0.5600
1:173635682:G:GA	donor_gain	0.5300
1:173635833:ACACC:A	acceptor_gain	0.5200
1:173635539:A:AA	donor_gain	0.5000
1:173635686:G:A	donor_gain	0.5000
1:173635771:CAAG:C	acceptor_gain	0.4900
1:173635835:ACC:A	acceptor_gain	0.4900
1:173635824:ATGG:A	acceptor_gain	0.4800
1:173635791:A:C	acceptor_gain	0.4700
1:173635823:AATGG:A	acceptor_gain	0.4700
1:173635679:A:AC	donor_gain	0.4400
1:173635680:C:CC	donor_gain	0.4400
1:173635790:T:TC	acceptor_gain	0.4300
1:173635836:CC:C	acceptor_gain	0.4100
1:173635834:CACC:C	acceptor_gain	0.4000
1:173635635:T:TA	donor_gain	0.3900
1:173635797:T:TG	acceptor_gain	0.3900
1:173635837:C:A	acceptor_gain	0.3900

AlphaMissense

1170 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:173635609:T:A	W30R	0.932
1:173635609:T:C	W30R	0.932
1:173635611:G:C	W30C	0.926
1:173635611:G:T	W30C	0.926
1:173635626:G:C	W35C	0.908
1:173635626:G:T	W35C	0.908
1:173635631:T:C	I37T	0.875
1:173635622:G:A	G34E	0.845
1:173635621:G:A	G34R	0.840
1:173635621:G:C	G34R	0.840
1:173635624:T:A	W35R	0.839
1:173635624:T:C	W35R	0.839
1:173635628:A:T	E36V	0.815
1:173635631:T:G	I37S	0.814
1:173635617:G:C	K32N	0.790
1:173635617:G:T	K32N	0.790
1:173635827:G:C	W102C	0.788
1:173635827:G:T	W102C	0.788
1:173635814:T:C	L98P	0.758
1:173635610:G:C	W30S	0.752
1:173635816:T:A	W99R	0.751
1:173635816:T:C	W99R	0.751
1:173635825:T:A	W102R	0.724
1:173635825:T:C	W102R	0.724
1:173635634:T:C	L38P	0.717
1:173635619:T:A	V33D	0.711
1:173635636:C:T	P39S	0.710
1:173635627:G:A	E36K	0.700
1:173635618:G:C	V33L	0.694
1:173635636:C:A	P39T	0.691

dbSNP variants (sampled 300 via entrez): RS1000003492 (1:173634713 A>G), RS1000269357 (1:173637181 G>A), RS1001005380 (1:173635213 T>G), RS1001075774 (1:173634861 C>A), RS1002009575 (1:173633995 T>C), RS1002427863 (1:173635958 C>A), RS1006087310 (1:173636885 A>G), RS1006697132 (1:173637288 A>C,G,T), RS1007594428 (1:173636425 C>A), RS1007796663 (1:173636723 T>C), RS1008481877 (1:173633515 C>T), RS1008814094 (1:173634957 A>G), RS1014139379 (1:173635638 A>C,G), RS1014352645 (1:173635530 T>A,C), RS1014382408 (1:173635931 C>T)

Disease associations

OMIM: gene MIM:621346 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

4 total (human), top 4 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
S-(1,2-dichlorovinyl)cysteine	affects response to substance, increases expression, affects cotreatment	1
Cadmium	decreases expression, increases abundance	1
Lipopolysaccharides	increases expression, affects cotreatment, affects response to substance	1
Cadmium Chloride	decreases expression, increases abundance	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.