TMED7

Summary

TMED7 (transmembrane p24 trafficking protein 7, HGNC:24253) is a protein-coding gene on chromosome 5q22.3, encoding Transmembrane emp24 domain-containing protein 7 (Q9Y3B3). Potential role in vesicular protein trafficking, mainly in the early secretory pathway.

Predicted to be involved in Golgi organization; endoplasmic reticulum to Golgi vesicle-mediated transport; and intracellular protein transport. Located in Golgi apparatus; endoplasmic reticulum; and endoplasmic reticulum-Golgi intermediate compartment.

Source: NCBI Gene 51014 — RefSeq curated summary.

At a glance

• Gene–disease (curated): retinal disorder (Limited, GenCC)
• GWAS associations: 4
• Clinical variants (ClinVar): 58 total
• Druggable target: yes
• MANE Select transcript: NM_181836

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24253
Approved symbol	TMED7
Name	transmembrane p24 trafficking protein 7
Location	5q22.3
Locus type	gene with protein product
Status	Approved
Aliases	CGI-109, FLJ90481, p24gamma3, p24g3
Ensembl gene	ENSG00000134970
Ensembl biotype	protein_coding
OMIM	619990
Entrez	51014

Gene structure

Transcript identifiers

Ensembl transcripts: 9 — 8 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000456936, ENST00000503010, ENST00000508420, ENST00000878959, ENST00000878960, ENST00000878961, ENST00000878962, ENST00000923920, ENST00000923921

RefSeq mRNA: 1 — MANE Select: NM_181836 NM_181836

CCDS: CCDS4120

Canonical transcript exons

ENST00000456936 — 3 exons

Exon	Start	End
ENSE00001756050	115613210	115616445
ENSE00002252373	115625601	115626036
ENSE00003515673	115620435	115620680

Expression profiles

Bgee: expression breadth ubiquitous, 291 present calls, max score 97.76.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 3.6221 / max 54.2742, expressed in 1571 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
63020	80.5731	1821
63021	2.9650	1419
63018	1.6400	1044
63023	0.9405	544
203660	0.7098	477
63017	0.3319	142

Top tissues by expression

294 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
choroid plexus epithelium	UBERON:0003911	97.76	gold quality
islet of Langerhans	UBERON:0000006	97.24	gold quality
calcaneal tendon	UBERON:0003701	97.13	gold quality
corpus epididymis	UBERON:0004359	97.06	gold quality
seminal vesicle	UBERON:0000998	96.83	gold quality
germinal epithelium of ovary	UBERON:0001304	96.61	gold quality
stromal cell of endometrium	CL:0002255	96.55	gold quality
palpebral conjunctiva	UBERON:0001812	96.46	gold quality
adrenal tissue	UBERON:0018303	96.36	gold quality
jejunal mucosa	UBERON:0000399	95.59	gold quality
caput epididymis	UBERON:0004358	95.30	gold quality
cauda epididymis	UBERON:0004360	95.14	gold quality
rectum	UBERON:0001052	95.03	gold quality
epithelium of nasopharynx	UBERON:0001951	94.97	gold quality
heart right ventricle	UBERON:0002080	94.87	gold quality
lower lobe of lung	UBERON:0008949	94.86	gold quality
jejunum	UBERON:0002115	94.72	gold quality
endometrium	UBERON:0001295	94.48	gold quality
cartilage tissue	UBERON:0002418	94.48	gold quality
pancreas	UBERON:0001264	94.38	gold quality
endothelial cell	CL:0000115	94.20	gold quality
descending thoracic aorta	UBERON:0002345	94.16	gold quality
monocyte	CL:0000576	94.07	gold quality
mononuclear cell	CL:0000842	94.01	gold quality
thoracic aorta	UBERON:0001515	93.96	gold quality
mucosa of sigmoid colon	UBERON:0004993	93.94	gold quality
ascending aorta	UBERON:0001496	93.93	gold quality
nasal cavity mucosa	UBERON:0001826	93.93	gold quality
pericardium	UBERON:0002407	93.86	gold quality
mucosa of paranasal sinus	UBERON:0005030	93.86	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	11.98

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

221 targeting TMED7, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-450A-1-3P	100.00	69.33	1837
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-12118	100.00	65.88	1270
HSA-MIR-6740-5P	100.00	65.64	932
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-3692-3P	99.98	70.27	2139
HSA-MIR-6891-5P	99.98	66.53	1372
HSA-MIR-3173-3P	99.98	66.49	1217
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-548AN	99.97	70.91	2817
HSA-MIR-570-3P	99.96	72.41	4910

Literature-anchored findings (GeneRIF, showing 2)

• TMED7 is essential for TAG-mediated disruption of the TRAM/TRIF complex and the degradation of toll-like receptor 4 (PMID:22426228)
• TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane (PMID:25074978)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	tmed7	ENSDARG00000046022
mus_musculus	Tmed7	ENSMUSG00000033184
drosophila_melanogaster	p24-1	FBGN0030341
caenorhabditis_elegans	tmed-3	WBGENE00019003

Paralogs (8): TMED2 (ENSG00000086598), TMED1 (ENSG00000099203), TMED5 (ENSG00000117500), TMED6 (ENSG00000157315), TMED4 (ENSG00000158604), TMED3 (ENSG00000166557), TMED10 (ENSG00000170348), TMED9 (ENSG00000184840)

Protein

Protein identifiers

Transmembrane emp24 domain-containing protein 7 — Q9Y3B3 (reviewed: Q9Y3B3)

Alternative names: p24 family protein gamma-3, p27

All UniProt accessions (2): G3V2Y2, Q9Y3B3

UniProt curated annotations — full annotation on UniProt →

Function. Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Appears to play a role in the biosynthesis of secreted cargo including processing and post-translational modifications.

Subunit / interactions. Predominantly monomeric and to lesser extent homodimeric in endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED9 and TMED10. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED7.

Subcellular location. Endoplasmic reticulum membrane. Golgi apparatus. cis-Golgi network membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cytoplasmic vesicle. COPI-coated vesicle membrane. COPII-coated vesicle membrane.

Post-translational modifications. N-linked glycosylated in complex form containing terminal sialic acid.

Similarity. Belongs to the EMP24/GP25L family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9Y3B3-1	1	yes
Q9Y3B3-2	2

RefSeq proteins (1): NP_861974* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR009038	GOLD_dom	Domain
IPR015720	Emp24-like	Family
IPR036598	GOLD_dom_sf	Homologous_superfamily

Pfam: PF01105

UniProt features (15 total): sequence conflict 5, topological domain 2, short sequence motif 2, signal peptide 1, chain 1, transmembrane region 1, domain 1, glycosylation site 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9Y3B3-F1	84.82	0.67

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Glycosylation sites (1): 103

Function

Pathways and Gene Ontology

Reactome pathways

2 pathways

ID	Pathway
R-HSA-6807878	COPI-mediated anterograde transport
R-HSA-6811434	COPI-dependent Golgi-to-ER retrograde traffic

MSigDB gene sets: 242 (showing top): GSE45365_NK_CELL_VS_CD8A_DC_MCMV_INFECTION_DN, BORCZUK_MALIGNANT_MESOTHELIOMA_UP, TAATAAT_MIR126, GOBP_INTRACELLULAR_PROTEIN_TRANSPORT, GOBP_VESICLE_MEDIATED_TRANSPORT, REACTOME_MEMBRANE_TRAFFICKING, GOLDRATH_ANTIGEN_RESPONSE, GOBP_ENDOPLASMIC_RETICULUM_TO_GOLGI_VESICLE_MEDIATED_TRANSPORT, GOCC_COATED_VESICLE, DAZARD_RESPONSE_TO_UV_SCC_UP, ATTCTTT_MIR186, GOCC_GOLGI_ASSOCIATED_VESICLE, GOCC_VESICLE_COAT, GOBP_ENDOMEMBRANE_SYSTEM_ORGANIZATION, TAATGTG_MIR323

GO Biological Process (4): intracellular protein transport (GO:0006886), endoplasmic reticulum to Golgi vesicle-mediated transport (GO:0006888), Golgi organization (GO:0007030), protein transport (GO:0015031)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (14): Golgi membrane (GO:0000139), endoplasmic reticulum (GO:0005783), endoplasmic reticulum membrane (GO:0005789), endoplasmic reticulum-Golgi intermediate compartment (GO:0005793), Golgi apparatus (GO:0005794), COPI vesicle coat (GO:0030126), COPII vesicle coat (GO:0030127), transport vesicle (GO:0030133), COPII-coated ER to Golgi transport vesicle (GO:0030134), endoplasmic reticulum-Golgi intermediate compartment membrane (GO:0033116), ER to Golgi transport vesicle membrane (GO:0012507), membrane (GO:0016020), COPI-coated vesicle membrane (GO:0030663), cytoplasmic vesicle (GO:0031410)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
ER to Golgi Anterograde Transport	1
Golgi-to-ER retrograde transport	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cytoplasm	5
endomembrane system	3
intracellular membrane-bounded organelle	3
intracellular protein localization	2
intracellular transport	2
Golgi apparatus	2
bounding membrane of organelle	2
vesicle coat	2
coated vesicle membrane	2
protein transport	1
intercellular transport	1
Golgi vesicle transport	1
organelle organization	1
endomembrane system organization	1
transport	1
establishment of protein localization	1
binding	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
COPI-coated vesicle membrane	1
ER to Golgi transport vesicle membrane	1
cytoplasmic vesicle	1
coated vesicle	1
endoplasmic reticulum-Golgi intermediate compartment	1
COPII-coated ER to Golgi transport vesicle	1
transport vesicle membrane	1
cellular anatomical structure	1
COPI-coated vesicle	1
Golgi-associated vesicle membrane	1
intracellular vesicle	1

Protein interactions and networks

STRING

1692 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TMED7	TMED6	Q8WW62	551
TMED7	TM9SF3	Q9HD45	448
TMED7	PIGG	Q5H8A4	429
TMED7	TIRAP	P58753	409
TMED7	ATP2B2	Q01814	387
TMED7	ATP2A1	O14983	386
TMED7	TMED10	P49755	382
TMED7	TMEM63A	O94886	381
TMED7	SSMEM1	Q8WWF3	375
TMED7	PHF7	Q9BWX1	373
TMED7	UNC80	Q8N2C7	364
TMED7	GPR83	Q9NYM4	353
TMED7	ATP2B3	Q16720	353
TMED7	TMED9	Q9BVK6	353
TMED7	KCNQ5	Q9NR82	353
TMED7	ATP2A3	Q93084	353
TMED7	CA6	P23280	353
TMED7	SH3GL2	Q99962	353

IntAct

75 interactions, top by confidence:

A	B	Type	Score
TMED10	TMED2	psi-mi:“MI:0914”(association)	0.910
GORASP2	GOLGA2	psi-mi:“MI:0914”(association)	0.880
TMED10	TMED1	psi-mi:“MI:0914”(association)	0.730
TMED7	TMED10	psi-mi:“MI:0914”(association)	0.730
TMED9	TMED10	psi-mi:“MI:0914”(association)	0.730
SURF4	TMED10	psi-mi:“MI:0915”(physical association)	0.660
TBP	TAF7	psi-mi:“MI:0914”(association)	0.640
TMED2	ATP9A	psi-mi:“MI:0914”(association)	0.640
PA2G4	TMED7	psi-mi:“MI:0915”(physical association)	0.640
ADGRG5	KLRG2	psi-mi:“MI:0914”(association)	0.530
SCN3B	ABCC5	psi-mi:“MI:0914”(association)	0.530
FGF9	PPID	psi-mi:“MI:0914”(association)	0.530
Gorasp1	CLIP-170	psi-mi:“MI:0915”(physical association)	0.400
TMED7	TMED7	psi-mi:“MI:0915”(physical association)	0.400
TMED7	SEC23A	psi-mi:“MI:0915”(physical association)	0.400
FGF16	TMED7	psi-mi:“MI:0915”(physical association)	0.400
TMPRSS3	TMED7	psi-mi:“MI:0915”(physical association)	0.370
Gorasp1	GOLGA2	psi-mi:“MI:0914”(association)	0.350
Tmed10	TARS3	psi-mi:“MI:0914”(association)	0.350
Tmed10	NDUFS8	psi-mi:“MI:0914”(association)	0.350
Tmed2		psi-mi:“MI:0914”(association)	0.350
Uso1	SLC30A6	psi-mi:“MI:0914”(association)	0.350
OCRL	MYO1C	psi-mi:“MI:0914”(association)	0.350
Cdx1	TAF7	psi-mi:“MI:0914”(association)	0.350

BioGRID (140): TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS), TMED7 (Affinity Capture-MS)

ESM2 similar proteins: A0JN27, A5PJI5, A5PKD9, B5DFK7, D3Z7P3, D3ZTX0, G3MWR8, G3V9T7, O43681, O54984, O94925, P13264, P16298, P23438, P43308, Q15303, Q15363, Q1LZ53, Q28D01, Q2HJ19, Q4V899, Q4W5Z4, Q5E9E4, Q5JUK3, Q5NVE6, Q5RIC0, Q5TA45, Q5XIJ5, Q5ZIH0, Q5ZJX1, Q61527, Q62956, Q63524, Q6AY25, Q6NVL5, Q6PCB6, Q6ZPR4, Q78IS1, Q7RTP6, Q8CJ19

Diamond homologs: A7SXK3, D3ZTX0, O17528, P32803, P49020, Q15363, Q3T133, Q54BN0, Q5I0E7, Q63524, Q6AY25, Q6BTC2, Q769F9, Q78IS1, Q7Z7H5, Q8R1V4, Q99KF1, Q9P7I9, Q9R0Q3, Q9Y3B3, Q9Y3Q3, O13770, Q13445, Q28BQ6, Q2KJ84, Q2TBK5, Q3V009, Q5BK85, Q5R7E6, Q5R809, Q6AXN3, Q9BVK6, Q9CXE7, Q9Y3A6, P39704, Q12403, Q9S7M9, Q12450, B3MTS8, B3NZM5

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 88 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
COPII-mediated vesicle transport	6	16.3×	4e-04
COPI-dependent Golgi-to-ER retrograde traffic	6	11.1×	2e-03
COPI-mediated anterograde transport	6	11.0×	2e-03

GO biological processes:

GO term	Partners	Fold	FDR
Golgi organization	10	18.3×	1e-07
endoplasmic reticulum to Golgi vesicle-mediated transport	8	14.9×	1e-05
transmembrane transport	5	11.5×	4e-03
intracellular protein transport	11	9.8×	4e-06

Disease & clinical

Clinical variants and AI predictions

ClinVar

58 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	32
Likely benign	23
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

374 predictions. Top by Δscore:

Variant	Effect	Δscore
5:115620677:TCAC:T	acceptor_gain	1.0000
5:115620678:CAC:C	acceptor_gain	1.0000
5:115620678:CACC:C	acceptor_gain	1.0000
5:115620679:ACCTG:A	acceptor_loss	1.0000
5:115620681:C:CA	acceptor_loss	1.0000
5:115620681:C:CC	acceptor_gain	1.0000
5:115620682:T:A	acceptor_loss	1.0000
5:115616443:CAT:C	acceptor_gain	0.9900
5:115616444:ATCTG:A	acceptor_loss	0.9900
5:115616445:TC:T	acceptor_loss	0.9900
5:115620676:ATCAC:A	acceptor_gain	0.9900
5:115620679:AC:A	acceptor_gain	0.9900
5:115620680:CC:C	acceptor_gain	0.9900
5:115620690:A:C	acceptor_gain	0.9900
5:115620691:T:C	acceptor_gain	0.9900
5:115620691:T:TC	acceptor_gain	0.9900
5:115625595:TGATA:T	donor_loss	0.9900
5:115625596:GATAC:G	donor_loss	0.9900
5:115625597:ATAC:A	donor_loss	0.9900
5:115625598:TA:T	donor_loss	0.9900
5:115625599:ACCTG:A	donor_loss	0.9900
5:115625600:C:CT	donor_loss	0.9900
5:115625600:C:G	donor_loss	0.9900
5:115616312:A:AC	donor_gain	0.9800
5:115616313:C:CC	donor_gain	0.9800
5:115616443:CATCT:C	acceptor_loss	0.9800
5:115616445:TCTG:T	acceptor_loss	0.9800
5:115616446:C:A	acceptor_loss	0.9800
5:115616446:C:CC	acceptor_gain	0.9800
5:115616447:T:A	acceptor_loss	0.9800

AlphaMissense

1452 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
5:115620513:T:A	K120N	1.000
5:115620513:T:G	K120N	1.000
5:115620514:T:A	K120I	1.000
5:115620531:A:C	F114L	1.000
5:115620531:A:T	F114L	1.000
5:115620533:A:G	F114L	1.000
5:115620537:A:C	N112K	1.000
5:115620537:A:T	N112K	1.000
5:115620538:T:A	N112I	1.000
5:115616281:G:C	S201R	0.999
5:115616281:G:T	S201R	0.999
5:115616283:T:G	S201R	0.999
5:115616349:C:G	A179P	0.999
5:115616357:C:G	R176P	0.999
5:115616367:C:G	A173P	0.999
5:115616378:C:G	R169P	0.999
5:115616411:A:G	L158P	0.999
5:115620508:A:T	V122E	0.999
5:115620515:T:C	K120E	0.999
5:115620529:G:A	S115F	0.999
5:115620532:A:C	F114C	0.999
5:115620539:T:A	N112Y	0.999
5:115620544:A:G	F110S	0.999
5:115620548:A:G	C109R	0.999
5:115620670:C:A	G68V	0.999
5:115620671:C:A	G68C	0.999
5:115625604:G:C	F63L	0.999
5:115625604:G:T	F63L	0.999
5:115625606:A:G	F63L	0.999
5:115616248:G:C	F212L	0.998

dbSNP variants (sampled 300 via entrez): RS1000002066 (5:115615435 T>C), RS1000272926 (5:115619220 T>C), RS1001226481 (5:115615001 C>T), RS1001264664 (5:115617528 A>G), RS1001304207 (5:115622416 C>G), RS1001351606 (5:115622025 A>C), RS1001403721 (5:115622291 A>G), RS1001680813 (5:115615342 A>G), RS1002371427 (5:115616851 C>G), RS1002641175 (5:115627135 T>C), RS1002968279 (5:115626861 CTT>C), RS1003177897 (5:115621894 A>G), RS1003228575 (5:115627043 C>T), RS1003235435 (5:115625991 G>A,C), RS1003309662 (5:115625040 G>C)

Disease associations

OMIM: gene MIM:619990 | disease phenotypes:

GenCC curated gene-disease

Disease	Classification	Inheritance
retinal disorder	Limited	Autosomal recessive

Mondo (1): retinal disorder (MONDO:0005283)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST004262_3	Liver injury in combined anti-retroviral and anti-tuberculosis drug-treated HIV with tuberculosis	4.000000e-06
GCST007059_14	Response to antidepressants (symptom improvement)	5.000000e-06
GCST012226_56	Waist circumference adjusted for body mass index	8.000000e-11
GCST012227_182	Hip circumference adjusted for BMI	8.000000e-13

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0007789	BMI-adjusted waist circumference
EFO:0008039	BMI-adjusted hip circumference

MeSH disease descriptors (1)

Descriptor	Name	Tree numbers
D012164	Retinal Diseases	C11.768

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067355 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.67	Kd	2125	nM	CHEMBL5653589
5.67	ED50	2125	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149603: Binding affinity to human TMED7 incubated for 45 mins by Kinobead based pull down assay	kd	2.1246	uM

CTD chemical–gene interactions

15 total (human), top 15 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cyclosporine	decreases methylation, increases expression	3
sodium arsenite	decreases expression	2
Tunicamycin	increases expression	2
Valproic Acid	increases expression	2
aristolochic acid I	decreases expression	1
bisphenol A	increases expression	1
abrine	decreases expression	1
jinfukang	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Cisplatin	decreases expression	1
Piroxicam	increases expression	1
Smoke	decreases expression, increases abundance	1
Tobacco Smoke Pollution	increases expression	1
Aflatoxin B1	decreases methylation	1
Aflatoxin M1	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652645	Binding	Binding affinity to human TMED7 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_TS73	HAP1 TMED7 (-) 1	Cancer cell line	Male
CVCL_XU34	HAP1 TMED7 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

27 trials via MONDO — disease-level, not drug-specific.

Trial	Phase	Status	Title
NCT01955135	PHASE4	COMPLETED	Anesthesia for Retinopathy of Prematurity
NCT01373476	PHASE2	COMPLETED	Multicentre, Randomized, Controlled Trial of Qideng Mingmu Capsule in The Treatment of Diabetic Retinopathy
NCT01793090	PHASE2	COMPLETED	EPI-743 in Cobalamin C Defect: Effects on Visual and Neurological Impairment
NCT04311112	PHASE2/PHASE3	WITHDRAWN	Safety and Efficacy of Zuretinol Acetate in Subjects With Inherited Retinal Disease
NCT04008121	EARLY_PHASE1	RECRUITING	Feasibility and Safety of MB-102 in Ocular Angiography as Compared to Fluorescein Sodium
NCT00259701	Not specified	COMPLETED	Microvascular Reactivity.
NCT00331370	Not specified	UNKNOWN	Hypertension Related Damage to the Microcirculation in South Asian: Emergence, Predictive Power and Reversibility
NCT00618644	Not specified	WITHDRAWN	Ranibizumab for Neovascularization in Sickle Cell Retinopathy
NCT00735657	Not specified	COMPLETED	Anesthesia for Pars Plana Vitrectomy (PPV) With Insulin Needle
NCT00828425	Not specified	COMPLETED	Management of Diabetes Mellitus Patients With Retinopathy
NCT00969956	Not specified	TERMINATED	Time To Complications Occurs in Diabetes
NCT01412905	Not specified	COMPLETED	Telemedicine Retinal Screening Utilizing a Mobile Medical Unit
NCT01546766	Not specified	COMPLETED	Rapid, Non-invasive, Regional Functional Imaging of the Retina. (Diabetic Retinopathy Diagnosis Device)
NCT01552993	Not specified	TERMINATED	Registration and Treatment of Pain During Eye Examination of Prematurity
NCT01815567	Not specified	COMPLETED	DETECT and Retinal Outcomes in Hypertension
NCT02321904	Not specified	COMPLETED	Corneal Confocal Microscopy to Detect Diabetic Neuropathy in Children
NCT02466607	Not specified	COMPLETED	Study of Stimulus Parameters in Flicker Electroretinogram (ERG)
NCT02558478	Not specified	UNKNOWN	Identification of New Genes Implicated in Rare Neurosensory Diseases by Whole Exome Sequencing
NCT02702973	Not specified	UNKNOWN	Characteristic Analysis of Retinopathy Associated With High Doses of Interferon α-2b Therapy
NCT03011541	Not specified	RECRUITING	Stem Cell Ophthalmology Treatment Study II
NCT03542734	Not specified	RECRUITING	Cognitive Impairment, Retinopathy, and Cerebrovascular Lesions in the Elderly
NCT03901898	Not specified	COMPLETED	Feasibility of an Intervention to Increase Diabetic Retinopathy Screening Attendance
NCT04819893	Not specified	RECRUITING	Study of the Involvement of Fatty Acids in Retinopathy of Prematurity: Relationship Between Retinopathy of Prematurity and the Rate of Expression of Transplacental Fatty Acid Receptors.
NCT05921981	Not specified	COMPLETED	Multisensory Stimulation Versus White Noise
NCT06239064	Not specified	ACTIVE_NOT_RECRUITING	Early Genetic Identification of Obesity
NCT06355219	Not specified	COMPLETED	Macrovascular and Microvascular Morbidity and Mortality After Metabolic Surgery Versus Medicines
NCT06837181	Not specified	RECRUITING	Studying the Presence of CFRD Complications With Thoughtful Recruitment (SPeCTRuM)