TMEM150A

Summary

TMEM150A (transmembrane protein 150A, HGNC:24677) is a protein-coding gene on chromosome 2p11.2, encoding Transmembrane protein 150A (Q86TG1). Regulates localization of phosphatidylinositol 4-kinase (PI4K) to the plasma membrane, possibly by reducing the association of TTC7 (TTC7A or TTC7B) with the PI4K complex.

Involved in phosphatidylinositol phosphate biosynthetic process and protein localization to plasma membrane. Located in plasma membrane.

Source: NCBI Gene 129303 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 32 total
• MANE Select transcript: NM_001031738

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24677
Approved symbol	TMEM150A
Name	transmembrane protein 150A
Location	2p11.2
Locus type	gene with protein product
Status	Approved
Aliases	DRAM5, TM6P1, FLJ90024, TTN1
Ensembl gene	ENSG00000168890
Ensembl biotype	protein_coding
OMIM	616757
Entrez	129303

Gene structure

Transcript identifiers

Ensembl transcripts: 21 — 12 protein_coding, 5 retained_intron, 4 nonsense_mediated_decay

ENST00000306353, ENST00000334462, ENST00000409668, ENST00000417791, ENST00000422458, ENST00000425160, ENST00000431593, ENST00000433956, ENST00000444380, ENST00000451147, ENST00000455852, ENST00000463363, ENST00000898691, ENST00000898692, ENST00000898693, ENST00000898694, ENST00000898695, ENST00000923606, ENST00000923607, ENST00000959579, ENST00000959580

RefSeq mRNA: 3 — MANE Select: NM_001031738 NM_001031738, NM_001369917, NM_153342

CCDS: CCDS33233

Canonical transcript exons

ENST00000334462 — 8 exons

Exon	Start	End
ENSE00001352072	85602607	85602698
ENSE00001596769	85598547	85599317
ENSE00003471361	85601435	85601482
ENSE00003514407	85600345	85600412
ENSE00003565619	85601021	85601107
ENSE00003598022	85601884	85602064
ENSE00003610468	85599891	85600018
ENSE00003670412	85599525	85599702

Expression profiles

Bgee: expression breadth ubiquitous, 237 present calls, max score 94.71.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 14.6014 / max 205.5660, expressed in 1789 samples.

FANTOM5 promoters (8 alternative TSS)

Promoter ID	TPM avg	Samples expressed
29428	6.7151	1733
29429	3.4750	1598
29427	2.5300	1287
29426	0.8472	576
29425	0.6229	375
29424	0.3993	218
29422	0.0075	4
29423	0.0044	2

Top tissues by expression

249 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
body of pancreas	UBERON:0001150	94.71	gold quality
gastrocnemius	UBERON:0001388	94.29	gold quality
right lobe of liver	UBERON:0001114	93.63	gold quality
pancreatic ductal cell	CL:0002079	93.56	silver quality
hindlimb stylopod muscle	UBERON:0004252	93.49	gold quality
muscle of leg	UBERON:0001383	93.21	gold quality
metanephros cortex	UBERON:0010533	92.50	gold quality
right testis	UBERON:0004534	91.78	gold quality
left testis	UBERON:0004533	91.63	gold quality
skeletal muscle organ	UBERON:0014892	91.36	gold quality
upper lobe of left lung	UBERON:0008952	91.25	gold quality
body of stomach	UBERON:0001161	91.07	gold quality
lower esophagus muscularis layer	UBERON:0035833	90.99	gold quality
lower esophagus	UBERON:0013473	90.97	gold quality
mucosa of stomach	UBERON:0001199	90.94	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	90.85	gold quality
adenohypophysis	UBERON:0002196	90.53	gold quality
cortex of kidney	UBERON:0001225	90.21	gold quality
adult mammalian kidney	UBERON:0000082	90.11	gold quality
muscle layer of sigmoid colon	UBERON:0035805	90.03	gold quality
right lung	UBERON:0002167	89.97	gold quality
right adrenal gland cortex	UBERON:0035827	89.97	gold quality
right lobe of thyroid gland	UBERON:0001119	89.92	gold quality
left uterine tube	UBERON:0001303	89.80	gold quality
right ovary	UBERON:0002118	89.75	gold quality
right adrenal gland	UBERON:0001233	89.73	gold quality
upper lobe of lung	UBERON:0008948	89.72	gold quality
left coronary artery	UBERON:0001626	89.62	gold quality
apex of heart	UBERON:0002098	89.60	gold quality
left lobe of thyroid gland	UBERON:0001120	89.55	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	9.77

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 2)

• TMEM150A is a functional homologue of Sfk1 in mammalian cells and plays a role in the homeostatic regulation of PI(4,5)P2 at the plasma membrane. (PMID:25608530)
• Overexpression of TMEM150A in glioblastoma multiforme patients correlated with dismal prognoses and compromised immune statuses. (PMID:38055673)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	tmem150ab	ENSDARG00000035184
danio_rerio	tmem150aa	ENSDARG00000068227
mus_musculus	Tmem150a	ENSMUSG00000055912
rattus_norvegicus	Tmem150a	ENSRNOG00000061100
caenorhabditis_elegans		WBGENE00008709

Paralogs (4): DRAM1 (ENSG00000136048), DRAM2 (ENSG00000156171), TMEM150B (ENSG00000180061), TMEM150C (ENSG00000249242)

Protein

Protein identifiers

Transmembrane protein 150A — Q86TG1 (reviewed: Q86TG1)

Alternative names: Transmembrane protein 150

All UniProt accessions (4): Q86TG1, C9JN48, F2Z311, F8VVL7

UniProt curated annotations — full annotation on UniProt →

Function. Regulates localization of phosphatidylinositol 4-kinase (PI4K) to the plasma membrane, possibly by reducing the association of TTC7 (TTC7A or TTC7B) with the PI4K complex. Acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis. May also play a role in fasting-induced catabolism.

Subunit / interactions. Interacts (via C-terminal cytoplasmic tail) with PI4KA.

Subcellular location. Cell membrane.

Similarity. Belongs to the DRAM/TMEM150 family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q86TG1-1	1	yes
Q86TG1-2	2

RefSeq proteins (3): NP_001026908, NP_001356846, NP_699173 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019402	CWH43_N	Domain
IPR050911	DRAM/TMEM150_Autophagy_Mod	Family

Pfam: PF10277

UniProt features (19 total): topological domain 7, transmembrane region 6, glycosylation site 2, splice variant 2, chain 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q86TG1-F1	90.17	0.82

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Glycosylation sites (2): 37, 41

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 120 (showing top): RRAGTTGT_UNKNOWN, GOBP_PHOSPHOLIPID_METABOLIC_PROCESS, GOBP_PHOSPHATIDYLINOSITOL_METABOLIC_PROCESS, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_UP, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_ORGANOPHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_PROTEIN_LOCALIZATION_TO_CELL_PERIPHERY, COUP_01, GOBP_PHOSPHOLIPID_BIOSYNTHETIC_PROCESS, GOBP_GLYCEROLIPID_METABOLIC_PROCESS, GTGCCTT_MIR506, UEDA_PERIFERAL_CLOCK, MYOD_01, KINSEY_TARGETS_OF_EWSR1_FLII_FUSION_DN

GO Biological Process (2): phosphatidylinositol phosphate biosynthetic process (GO:0046854), protein localization to plasma membrane (GO:0072659)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (2): plasma membrane (GO:0005886), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
glycerophospholipid biosynthetic process	1
protein localization to membrane	1
protein localization to cell periphery	1
binding	1
membrane	1
cell periphery	1
cellular anatomical structure	1

Protein interactions and networks

STRING

342 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TMEM150A	TTC7A	Q9ULT0	724
TMEM150A	TTC7B	Q86TV6	666
TMEM150A	EFR3B	Q9Y2G0	636
TMEM150A	HYCC1	Q9BYI3	633
TMEM150A	EFR3A	Q14156	543
TMEM150A	FBXL15	Q9H469	497
TMEM150A	PI4KA	P42356	493
TMEM150A	SH3BGRL	O75368	464
TMEM150A	CUEDC2	Q9H467	448
TMEM150A	TTN	Q8WZ42	447
TMEM150A	RPS6KA6	Q9UK32	445
TMEM150A	OXR1	Q8N573	434
TMEM150A	RNF181	Q9P0P0	429
TMEM150A	HSPH1	Q92598	427
TMEM150A	SLC25A44	Q96H78	424

IntAct

20 interactions, top by confidence:

A	B	Type	Score
KRTAP10-8	TMEM150A	psi-mi:“MI:0915”(physical association)	0.560
TMEM150A	ADAMTSL4	psi-mi:“MI:0915”(physical association)	0.560
NOTCH2NLA	TMEM150A	psi-mi:“MI:0915”(physical association)	0.560
TMEM150A	KRTAP10-8	psi-mi:“MI:0915”(physical association)	0.560
ADAMTSL4	TMEM150A	psi-mi:“MI:0915”(physical association)	0.560
TMEM150A	NOTCH2NLA	psi-mi:“MI:0915”(physical association)	0.560
TMEM150A	TRIM42	psi-mi:“MI:0915”(physical association)	0.560
NOTCH2NLC	TMEM150A	psi-mi:“MI:0915”(physical association)	0.560
TMEM150A	GOSR1	psi-mi:“MI:0914”(association)	0.350
TMEM150A	ACOX3	psi-mi:“MI:0914”(association)	0.350
TMEM150A	ACSL4	psi-mi:“MI:0914”(association)	0.350
TMEM150A	NOTCH2NLC	psi-mi:“MI:0915”(physical association)	0.000
pheS	TMEM150A	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (56): TMEM150A (Two-hybrid), KRTAP10-8 (Two-hybrid), NOTCH2NL (Two-hybrid), YY1 (Affinity Capture-MS), CCNT1 (Affinity Capture-MS), HELLS (Affinity Capture-MS), ACOX3 (Affinity Capture-MS), MIS18A (Affinity Capture-MS), ACOX3 (Affinity Capture-MS), CCNT1 (Affinity Capture-MS), YY1 (Affinity Capture-MS), MIS18A (Affinity Capture-MS), GOSR1 (Affinity Capture-MS), HELLS (Affinity Capture-MS), TMEM150A (Two-hybrid)

ESM2 similar proteins: A2AE42, A3A9H6, A5D7C9, A5D9A7, A6NM10, B3SHH9, B5DFH9, B9EJG8, F1NZP5, O14569, P10897, P49447, P82352, Q08DE1, Q14714, Q148G2, Q3ZCD2, Q5E965, Q5ND56, Q5RCZ2, Q5U2W7, Q5ZJX0, Q60720, Q62147, Q641Y1, Q6GPL4, Q6P0C6, Q6P1H1, Q71RH2, Q7TNV1, Q80ZE4, Q86TG1, Q8BMD6, Q8C8S3, Q8IXF9, Q8N8Q1, Q8NBI2, Q8TBR7, Q8VHW3, Q8VHW7

Diamond homologs: A5D7C9, A6NC51, A7MBB3, A9JSP6, B5DFH9, B9EJG8, Q28BP2, Q32PK2, Q3ZC48, Q4V7T3, Q4V7T7, Q5BK09, Q6GPL4, Q6UX65, Q86TG1, Q8C8S3, Q8R218, Q91WN2, Q9CR48, Q9DC58, Q9QZE9, Q8N682, Q5EAK8, Q6NRS6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

32 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	21
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1533 predictions. Top by Δscore:

Variant	Effect	Δscore
2:85599511:T:TA	donor_gain	1.0000
2:85599523:A:AC	donor_gain	1.0000
2:85599524:C:CC	donor_gain	1.0000
2:85599887:GCA:G	donor_loss	1.0000
2:85599905:C:CT	donor_gain	1.0000
2:85600014:GGCCA:G	acceptor_gain	1.0000
2:85600015:GCCA:G	acceptor_gain	1.0000
2:85600016:CCA:C	acceptor_gain	1.0000
2:85600016:CCAC:C	acceptor_gain	1.0000
2:85600017:CA:C	acceptor_gain	1.0000
2:85600017:CAC:C	acceptor_gain	1.0000
2:85600017:CACTG:C	acceptor_loss	1.0000
2:85600019:C:CC	acceptor_gain	1.0000
2:85600019:C:T	acceptor_loss	1.0000
2:85600409:CTTG:C	acceptor_gain	1.0000
2:85600413:C:CC	acceptor_gain	1.0000
2:85601015:CCTTA:C	donor_loss	1.0000
2:85601016:CTTA:C	donor_loss	1.0000
2:85601017:TTAC:T	donor_loss	1.0000
2:85601018:TAC:T	donor_loss	1.0000
2:85601019:A:AT	donor_loss	1.0000
2:85599497:T:A	donor_gain	0.9900
2:85599904:A:AC	donor_gain	0.9900
2:85599906:C:CC	donor_gain	0.9900
2:85600017:C:T	acceptor_gain	0.9900
2:85601019:A:AC	donor_gain	0.9900
2:85601020:C:CC	donor_gain	0.9900
2:85601105:GACCT:G	acceptor_loss	0.9900
2:85601106:ACC:A	acceptor_loss	0.9900
2:85601107:CCTG:C	acceptor_loss	0.9900

AlphaMissense

1759 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
2:85599258:A:G	W212R	0.995
2:85599258:A:T	W212R	0.995
2:85601897:C:G	G18R	0.994
2:85599256:C:A	W212C	0.993
2:85599256:C:G	W212C	0.993
2:85601919:G:C	S10R	0.993
2:85601919:G:T	S10R	0.993
2:85601921:T:G	S10R	0.993
2:85601926:G:T	P8H	0.993
2:85601939:A:G	W4R	0.993
2:85601939:A:T	W4R	0.993
2:85599894:A:C	F131L	0.992
2:85599894:A:T	F131L	0.992
2:85599896:A:G	F131L	0.992
2:85600373:G:C	S80R	0.992
2:85600373:G:T	S80R	0.992
2:85600375:T:G	S80R	0.992
2:85601891:A:G	W20R	0.992
2:85601891:A:T	W20R	0.992
2:85601896:C:T	G18D	0.992
2:85599205:A:C	F229L	0.991
2:85599205:A:T	F229L	0.991
2:85599207:A:G	F229L	0.991
2:85599934:C:T	G118D	0.991
2:85599935:C:G	G118R	0.991
2:85600376:G:C	F79L	0.990
2:85600376:G:T	F79L	0.990
2:85600378:A:G	F79L	0.990
2:85600388:T:A	E75D	0.990
2:85600388:T:G	E75D	0.990

dbSNP variants (sampled 300 via entrez): RS1000143101 (2:85598708 C>T), RS1000730365 (2:85599764 C>T), RS1001842985 (2:85598594 C>T), RS1002387368 (2:85598988 T>A,C,G), RS1003606506 (2:85598242 T>C), RS1003680140 (2:85598632 G>A), RS1004296214 (2:85600097 T>C), RS1005298596 (2:85598555 A>G), RS1005328244 (2:85598180 C>T), RS1005661760 (2:85601097 C>G,T), RS1006148376 (2:85601572 C>G,T), RS1006195120 (2:85604023 G>A), RS1006505771 (2:85603423 A>C), RS1006635104 (2:85603759 A>C,G), RS1007672018 (2:85604021 T>A)

Disease associations

OMIM: gene MIM:616757 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

32 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cyclosporine	decreases expression, decreases methylation	3
sodium arsenite	decreases expression, affects cotreatment, increases abundance	2
Acetaminophen	decreases expression	2
Valproic Acid	affects expression, decreases expression	2
afuresertib	increases expression	1
bufotalin	decreases expression	1
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
beta-lapachone	decreases expression, increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
manganese chloride	decreases expression, increases abundance, affects cotreatment	1
di-n-butylphosphoric acid	affects expression	1
perfluorooctane sulfonic acid	increases expression	1
nutlin 3	affects cotreatment, increases expression	1
ICG 001	increases expression	1
jinfukang	affects cotreatment, increases expression	1
Resveratrol	decreases expression, affects cotreatment	1
Arsenic	affects cotreatment, decreases expression, increases abundance	1
Cisplatin	affects cotreatment, increases expression	1
Coumestrol	decreases expression	1
Dactinomycin	affects cotreatment, increases expression	1
Dichlorodiphenyl Dichloroethylene	decreases expression	1
Manganese	decreases expression, increases abundance, affects cotreatment	1
Plant Extracts	affects cotreatment, decreases expression	1
Silicon Dioxide	increases expression	1
Smoke	decreases expression	1
Thiram	decreases expression	1
Tobacco Smoke Pollution	affects expression	1
Aflatoxin B1	decreases expression	1
Cadmium Chloride	decreases expression	1

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_TS77	HAP1 TMEM150A (-) 1	Cancer cell line	Male
CVCL_TS78	HAP1 TMEM150A (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.