TMEM150B

Summary

TMEM150B (transmembrane protein 150B, HGNC:34415) is a protein-coding gene on chromosome 19q13.42, encoding Modulator of macroautophagy TMEM150B (A6NC51). Modulator of macroautophagy that causes accumulation of autophagosomes under basal conditions and enhances autophagic flux.

This gene encodes a protein that belongs to the DRAM (damage-regulated autophagy modulator) family of membrane-spanning proteins. Alternate splicing results in multiple transcript variants.

Source: NCBI Gene 284417 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 55 total
• MANE Select transcript: NM_001282011

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:34415
Approved symbol	TMEM150B
Name	transmembrane protein 150B
Location	19q13.42
Locus type	gene with protein product
Status	Approved
Aliases	TTN2, DRAM3
Ensembl gene	ENSG00000180061
Ensembl biotype	protein_coding
OMIM	617291
Entrez	284417

Gene structure

Transcript identifiers

Ensembl transcripts: 7 — 3 protein_coding, 3 nonsense_mediated_decay, 1 retained_intron

ENST00000326652, ENST00000585918, ENST00000586609, ENST00000591570, ENST00000592603, ENST00000592731, ENST00000592891

RefSeq mRNA: 2 — MANE Select: NM_001282011 NM_001085488, NM_001282011

CCDS: CCDS42629

Canonical transcript exons

ENST00000326652 — 8 exons

Exon	Start	End
ENSE00001431646	55322648	55322743
ENSE00001750376	55325272	55325341
ENSE00003487175	55312801	55313055
ENSE00003519784	55316786	55316966
ENSE00003546299	55320969	55321093
ENSE00003606385	55320391	55320458
ENSE00003610829	55320558	55320617
ENSE00003660752	55320039	55320166

Expression profiles

Bgee: expression breadth ubiquitous, 150 present calls, max score 92.68.

FANTOM5 (CAGE): breadth broad, TPM avg 3.5449 / max 169.2603, expressed in 360 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
182786	3.1588	341
182785	0.3764	160
182778	0.0098	4

Top tissues by expression

240 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
monocyte	CL:0000576	92.68	gold quality
leukocyte	CL:0000738	91.98	gold quality
granulocyte	CL:0000094	89.31	gold quality
small intestine Peyer’s patch	UBERON:0003454	85.94	gold quality
small intestine	UBERON:0002108	83.27	gold quality
mucosa of transverse colon	UBERON:0004991	82.83	gold quality
upper lobe of left lung	UBERON:0008952	79.28	gold quality
transverse colon	UBERON:0001157	79.10	gold quality
duodenum	UBERON:0002114	78.77	gold quality
right lung	UBERON:0002167	78.42	gold quality
rectum	UBERON:0001052	77.60	gold quality
vermiform appendix	UBERON:0001154	77.18	gold quality
spleen	UBERON:0002106	77.09	gold quality
upper lobe of lung	UBERON:0008948	76.51	gold quality
blood	UBERON:0000178	74.80	gold quality
right coronary artery	UBERON:0001625	73.38	gold quality
omental fat pad	UBERON:0010414	72.91	gold quality
peritoneum	UBERON:0002358	72.82	gold quality
gall bladder	UBERON:0002110	72.23	gold quality
intestine	UBERON:0000160	71.45	gold quality
right adrenal gland	UBERON:0001233	71.24	gold quality
adipose tissue of abdominal region	UBERON:0007808	71.18	gold quality
right adrenal gland cortex	UBERON:0035827	71.18	gold quality
left adrenal gland	UBERON:0001234	70.62	gold quality
left adrenal gland cortex	UBERON:0035825	70.48	gold quality
bone marrow cell	CL:0002092	69.32	silver quality
caecum	UBERON:0001153	69.26	gold quality
smooth muscle tissue	UBERON:0001135	68.89	gold quality
lymph node	UBERON:0000029	68.68	gold quality
adrenal cortex	UBERON:0001235	68.51	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.36

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 3)

• TMEM150B, renamed as DRAM-3, is a modulator of both macroautophagy and cell survival under starvation conditions. (PMID:25929859)
• TMEM150B expression is significantly upregulated in human masticatory mucosa during wound healing (PMID:28005267)
• The results suggest that the perturbations in the TMEM150B gene are not a common explanation for premature ovarian insufficiency in Chinese women. (PMID:30704953)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	tmem150b	ENSDARG00000056144
mus_musculus	Tmem150b	ENSMUSG00000046456
rattus_norvegicus	Tmem150b	ENSRNOG00000028273
caenorhabditis_elegans		WBGENE00008709

Paralogs (4): DRAM1 (ENSG00000136048), DRAM2 (ENSG00000156171), TMEM150A (ENSG00000168890), TMEM150C (ENSG00000249242)

Protein

Protein identifiers

Modulator of macroautophagy TMEM150B — A6NC51 (reviewed: A6NC51)

Alternative names: Protein DRAM-3, Transmembrane protein 150B, Transmembrane protein 224

All UniProt accessions (5): A6NC51, K7EKL2, K7EKW5, K7EM00, K7ENI3

UniProt curated annotations — full annotation on UniProt →

Function. Modulator of macroautophagy that causes accumulation of autophagosomes under basal conditions and enhances autophagic flux. Represses cell death and promotes long-term clonogenic survival of cells grown in the absence of glucose in a macroautophagy-independent manner. May have some role in extracellular matrix engulfment or growth factor receptor recycling, both of which can modulate cell survival.

Subcellular location. Cell membrane. Endosome membrane. Cytoplasmic vesicle. Autophagosome membrane.

Tissue specificity. Highly expressed in the colon and lung with comparatively high levels also detectable in the lymph nodes, placenta, duodenum, peripheral blood mononuclear cells and spleen.

Induction. Is not markedly up- or down-regulated by DNA damage, nutrient deprivation or by exposure to TNF and IFN-gamma.

Similarity. Belongs to the DRAM/TMEM150 family.

RefSeq proteins (2): NP_001078957, NP_001268940* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019402	CWH43_N	Domain
IPR050911	DRAM/TMEM150_Autophagy_Mod	Family

Pfam: PF10277

UniProt features (16 total): topological domain 7, transmembrane region 6, chain 1, glycosylation site 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A6NC51-F1	93.12	0.84

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Glycosylation sites (1): 30

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 43 (showing top): GSE45365_NK_CELL_VS_CD8A_DC_DN, GOCC_VACUOLAR_MEMBRANE, GOCC_AUTOPHAGOSOME, GOCC_AUTOPHAGOSOME_MEMBRANE, CHYLA_CBFA2T3_TARGETS_UP, GOBP_PROCESS_UTILIZING_AUTOPHAGIC_MECHANISM, GOCC_ENDOSOME_MEMBRANE, MAFG_TARGET_GENES, GSE15733_BM_VS_SPLEEN_MEMORY_CD4_TCELL_UP, GSE15767_MED_VS_SCS_MAC_LN_UP, GSE12003_MIR223_KO_VS_WT_BM_PROGENITOR_4D_CULTURE_DN, DESCARTES_MAIN_FETAL_PDE1C_ACSM3_POSITIVE_CELLS, DESCARTES_FETAL_ADRENAL_MYELOID_CELLS, LHX2_TARGET_GENES, AEBP2_TARGET_GENES

GO Biological Process (1): autophagy (GO:0006914)

GO Molecular Function (0):

GO Cellular Component (6): autophagosome membrane (GO:0000421), plasma membrane (GO:0005886), endosome membrane (GO:0010008), endosome (GO:0005768), membrane (GO:0016020), cytoplasmic vesicle (GO:0031410)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
catabolic process	1
transmembrane transport	1
process utilizing autophagic mechanism	1
vacuolar membrane	1
autophagosome	1
membrane	1
cell periphery	1
endosome	1
cytoplasmic vesicle membrane	1
bounding membrane of organelle	1
endomembrane system	1
cytoplasmic vesicle	1
cellular anatomical structure	1
cytoplasm	1
intracellular vesicle	1

Protein interactions and networks

STRING

232 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TMEM150B	BRSK1	Q8TDC3	631
TMEM150B	MCM8	Q9UJA3	621
TMEM150B	SYCP2L	Q5T4T6	610
TMEM150B	A0A494C100	A0A494C100	603
TMEM150B	UIMC1	Q96RL1	573
TMEM150B	ZNF518A	Q6AHZ1	467
TMEM150B	LRRC61	Q9BV99	432
TMEM150B	ZCCHC2	Q9C0B9	421
TMEM150B	NLRP11	P59045	399
TMEM150B	PRIM1	P49642	374
TMEM150B	HK3	P52790	373
TMEM150B	Q6GMV1	Q6GMV1	372
TMEM150B	CCT8L2	Q96SF2	371
TMEM150B	TLK1	Q9UKI8	370
TMEM150B	NOL4	O94818	362

IntAct

0 interactions, top by confidence:

BioGRID (1): TMEM150B (Affinity Capture-MS)

ESM2 similar proteins: A2A559, A2V7M9, A6H7B8, A6NC51, A6X919, A7MBB3, A7YWP2, A8KBG2, A8WFS8, A8XST1, A9JSP6, D4AD75, P70245, Q0VFE3, Q22141, Q29M88, Q2ABP2, Q2ABP3, Q2PZI1, Q32PK2, Q3TQR0, Q3ZC48, Q4V7T3, Q4V7T7, Q4VV71, Q568I2, Q5BK09, Q5BL33, Q5EAK8, Q5M9A7, Q60774, Q63175, Q68EV0, Q6NRS6, Q6P0S3, Q6UX65, Q6ZMB5, Q7K0P4, Q8BHJ6, Q8N682

Diamond homologs: A5D7C9, A6NC51, A9JSP6, B5DFH9, B9EJG8, Q28BP2, Q32PK2, Q3ZC48, Q4V7T3, Q4V7T7, Q6UX65, Q8C8S3, Q8R218, A7MBB3, Q5BK09, Q6GPL4, Q86TG1, Q91WN2, Q9CR48, Q9DC58, Q9QZE9, Q8N682, Q5EAK8, Q6NRS6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

55 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	46
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1668 predictions. Top by Δscore:

Variant	Effect	Δscore
19:55320618:C:CC	acceptor_gain	1.0000
19:55316786:TGGC:T	donor_gain	0.9900
19:55320554:TTACC:T	donor_loss	0.9900
19:55320613:CAAAA:C	acceptor_gain	0.9900
19:55320614:AAAA:A	acceptor_gain	0.9900
19:55322642:GCTCA:G	donor_loss	0.9900
19:55322643:CTCAC:C	donor_loss	0.9900
19:55322644:TCA:T	donor_loss	0.9900
19:55322645:CA:C	donor_loss	0.9900
19:55322646:ACC:A	donor_loss	0.9900
19:55322647:CCT:C	donor_gain	0.9900
19:55322740:CATC:C	acceptor_gain	0.9900
19:55320162:CGCGG:C	acceptor_gain	0.9800
19:55320615:AAA:A	acceptor_gain	0.9800
19:55320615:AAAC:A	acceptor_loss	0.9800
19:55320616:AA:A	acceptor_gain	0.9800
19:55320967:A:AC	donor_gain	0.9800
19:55320968:C:CC	donor_gain	0.9800
19:55320968:CA:C	donor_gain	0.9800
19:55322659:T:TA	donor_gain	0.9800
19:55322741:ATCCT:A	acceptor_loss	0.9800
19:55322742:TCCTG:T	acceptor_loss	0.9800
19:55322743:CCTGC:C	acceptor_loss	0.9800
19:55322744:CTGCA:C	acceptor_loss	0.9800
19:55322745:T:C	acceptor_loss	0.9800
19:55316784:A:AC	donor_gain	0.9700
19:55316785:C:CC	donor_gain	0.9700
19:55321045:C:CC	acceptor_gain	0.9700
19:55322742:TC:T	acceptor_gain	0.9700
19:55322743:CC:C	acceptor_gain	0.9700

AlphaMissense

1500 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:55320042:G:C	F107L	0.997
19:55320042:G:T	F107L	0.997
19:55320044:A:G	F107L	0.997
19:55320045:A:C	N106K	0.996
19:55320045:A:T	N106K	0.996
19:55320422:G:C	F55L	0.994
19:55320422:G:T	F55L	0.994
19:55320424:A:G	F55L	0.994
19:55313005:A:G	W186R	0.991
19:55313005:A:T	W186R	0.991
19:55320407:A:C	N60K	0.991
19:55320407:A:T	N60K	0.991
19:55320419:G:C	S56R	0.991
19:55320419:G:T	S56R	0.991
19:55320421:T:G	S56R	0.991
19:55313006:C:A	E185D	0.990
19:55313006:C:G	E185D	0.990
19:55316945:G:C	H116D	0.990
19:55320561:A:G	I42T	0.990
19:55320976:A:G	W21R	0.990
19:55320976:A:T	W21R	0.990
19:55320043:A:G	F107S	0.988
19:55320614:A:C	F24L	0.988
19:55320614:A:T	F24L	0.988
19:55320616:A:G	F24L	0.988
19:55320049:C:T	G105D	0.987
19:55313003:C:A	W186C	0.986
19:55313003:C:G	W186C	0.986
19:55313007:T:A	E185V	0.986
19:55316943:G:C	H116Q	0.986

dbSNP variants (sampled 300 via entrez): RS1000172205 (19:55312723 G>A), RS1000656340 (19:55323030 C>T), RS1000985013 (19:55322285 G>T), RS1001017860 (19:55322474 G>A), RS1001208965 (19:55313584 A>C), RS1001485330 (19:55322590 T>C), RS1001490473 (19:55320280 T>C), RS1001573376 (19:55314425 T>G), RS1001939466 (19:55313269 G>A), RS1002089558 (19:55325482 C>T), RS1002360568 (19:55317364 G>A), RS1002535689 (19:55314454 T>A,G), RS1002549151 (19:55308970 C>G,T), RS1002773780 (19:55312065 C>A,T), RS1003023043 (19:55320069 G>T)

Disease associations

OMIM: gene MIM:617291 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST000401_6	Menopause (age at onset)	6.000000e-11
GCST001381_6	Menopause (age at onset)	1.000000e-59
GCST005547_2	Major depressive disorder	5.000000e-11
GCST90011899_70	Aspartate aminotransferase levels	3.000000e-08

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0004704	age at menopause
EFO:0004736	aspartate aminotransferase measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

6 total (human), top 6 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	decreases expression, decreases methylation	2
sodium arsenite	decreases expression	1
(+)-JQ1 compound	decreases expression	1
theaflavin-3,3’-digallate	affects expression	1
Leflunomide	increases expression	1
Okadaic Acid	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.