TMEM150C

Summary

TMEM150C (transmembrane protein 150C, HGNC:37263) is a protein-coding gene on chromosome 4q21.22, encoding Transmembrane protein 150C (B9EJG8). Nonselective cationic channel with high permeability to Ca(2+).

This gene encodes a transmembrane protein component of a mechanosensitve ion channel that is activated by mechanical stimuli in various cell types and confers slowly adapting, mechanically activated currents in dorsal root ganglion neurons. Mechanically activated ion channels are sensors that are critical for hearing, touch, pain, and blood pressure regulation.

Source: NCBI Gene 441027 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 29 total
• MANE Select transcript: NM_001080506

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:37263
Approved symbol	TMEM150C
Name	transmembrane protein 150C
Location	4q21.22
Locus type	gene with protein product
Status	Approved
Aliases	DRAM4, FLJ12993, TTN3
Ensembl gene	ENSG00000249242
Ensembl biotype	protein_coding
OMIM	617292
Entrez	441027

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 4 protein_coding

ENST00000449862, ENST00000454948, ENST00000508701, ENST00000515780

RefSeq mRNA: 3 — MANE Select: NM_001080506 NM_001080506, NM_001353454, NM_001353455

CCDS: CCDS47087

Canonical transcript exons

ENST00000449862 — 8 exons

Exon	Start	End
ENSE00001618366	82490061	82490238
ENSE00001633635	82496068	82496195
ENSE00001650997	82502895	82502927
ENSE00001667588	82504578	82504667
ENSE00001674009	82483176	82485719
ENSE00001696540	82502727	82502794
ENSE00001708206	82503059	82503112
ENSE00002084189	82561906	82561988

Expression profiles

Bgee: expression breadth ubiquitous, 251 present calls, max score 99.00.

FANTOM5 (CAGE): breadth broad, TPM avg 4.8078 / max 173.3095, expressed in 790 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
52856	3.9328	743
52860	0.3543	172
52859	0.2952	157
52857	0.2017	116
52858	0.0238	8

Top tissues by expression

255 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
corpus epididymis	UBERON:0004359	99.00	gold quality
cauda epididymis	UBERON:0004360	98.09	gold quality
Brodmann (1909) area 23	UBERON:0013554	95.58	gold quality
caput epididymis	UBERON:0004358	93.87	gold quality
left ventricle myocardium	UBERON:0006566	93.45	gold quality
kidney epithelium	UBERON:0004819	92.94	silver quality
buccal mucosa cell	CL:0002336	92.40	gold quality
right adrenal gland cortex	UBERON:0035827	91.97	gold quality
adrenal cortex	UBERON:0001235	91.68	gold quality
cortical plate	UBERON:0005343	91.68	gold quality
left adrenal gland	UBERON:0001234	91.63	gold quality
left adrenal gland cortex	UBERON:0035825	91.50	gold quality
germinal epithelium of ovary	UBERON:0001304	91.34	gold quality
mucosa of paranasal sinus	UBERON:0005030	90.88	gold quality
bronchial epithelial cell	CL:0002328	90.78	gold quality
entorhinal cortex	UBERON:0002728	90.73	gold quality
cardiac muscle of right atrium	UBERON:0003379	90.59	silver quality
right adrenal gland	UBERON:0001233	90.39	gold quality
superior frontal gyrus	UBERON:0002661	90.39	gold quality
bronchus	UBERON:0002185	90.30	gold quality
middle temporal gyrus	UBERON:0002771	89.62	gold quality
parotid gland	UBERON:0001831	89.45	gold quality
heart right ventricle	UBERON:0002080	89.25	gold quality
adrenal gland	UBERON:0002369	89.07	gold quality
endothelial cell	CL:0000115	88.98	gold quality
medial globus pallidus	UBERON:0002477	88.92	gold quality
primary visual cortex	UBERON:0002436	88.78	gold quality
tibia	UBERON:0000979	88.52	gold quality
occipital lobe	UBERON:0002021	88.21	gold quality
tendon of biceps brachii	UBERON:0008188	88.17	gold quality

Single-cell (SCXA)

Detected in 4 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-HCAD-38	yes	427.95
E-GEOD-81608	yes	16.45
E-ANND-3	yes	14.21
E-GEOD-99795	no	3.39

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

48 targeting TMEM150C, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-574-5P	100.00	66.01	989
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-23A-3P	99.95	74.24	3163
HSA-MIR-23B-3P	99.95	74.24	3163
HSA-MIR-23C	99.95	73.92	3192
HSA-MIR-96-5P	99.95	72.80	2140
HSA-MIR-218-5P	99.93	72.22	2103
HSA-MIR-335-3P	99.93	73.36	4958
HSA-MIR-12133	99.92	71.82	2006
HSA-MIR-10527-5P	99.91	72.28	3754
HSA-MIR-1271-5P	99.91	71.99	1972
HSA-MIR-605-3P	99.88	69.22	1833
HSA-MIR-182-5P	99.87	74.03	2589
HSA-MIR-30A-3P	99.87	69.74	2928
HSA-MIR-30D-3P	99.87	69.92	2917
HSA-MIR-30E-3P	99.87	69.68	2942
HSA-MIR-6844	99.82	70.69	2423
HSA-MIR-636	99.80	69.58	1500
HSA-MIR-4679	99.76	69.19	1229
HSA-MIR-4306	99.72	70.50	3630
HSA-MIR-377-5P	99.70	65.28	712
HSA-MIR-6086	99.70	65.38	699
HSA-MIR-4530	99.69	66.47	1509
HSA-MIR-4328	99.57	71.06	4094
HSA-MIR-92B-5P	99.36	63.29	110
HSA-MIR-4644	99.35	69.12	2514
HSA-MIR-185-5P	99.35	68.60	2497
HSA-MIR-942-3P	98.81	69.04	876

Literature-anchored findings (GeneRIF, showing 2)

• Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics. (PMID:38124148)
• Tentonin 3 is a pore-forming subunit of a slow inactivation mechanosensitive channel. (PMID:38850532)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	tmem150c	ENSDARG00000069590
mus_musculus	Tmem150c	ENSMUSG00000050640
rattus_norvegicus	Tmem150c	ENSRNOG00000002258
caenorhabditis_elegans		WBGENE00008709

Paralogs (4): DRAM1 (ENSG00000136048), DRAM2 (ENSG00000156171), TMEM150A (ENSG00000168890), TMEM150B (ENSG00000180061)

Protein

Protein identifiers

Transmembrane protein 150C — B9EJG8 (reviewed: B9EJG8)

Alternative names: Tentonin 3

All UniProt accessions (3): B9EJG8, D6RAQ9, D6RDW6

UniProt curated annotations — full annotation on UniProt →

Function. Nonselective cationic channel with high permeability to Ca(2+). Component of a mechanosensitive cation channel, confers mechanically activated (MA) currents with slow inactivation kinetics. May contribute to proprioception.

Subcellular location. Cell membrane. Lysosome membrane.

Miscellaneous. Tentonin comes from the Greek ’tentono’ meaning to stretch.

Similarity. Belongs to the DRAM/TMEM150 family.

Isoforms (2)

UniProt ID	Names	Canonical?
B9EJG8-1	1	yes
B9EJG8-2	2

RefSeq proteins (3): NP_001073975, NP_001340383, NP_001340384 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019402	CWH43_N	Domain
IPR050911	DRAM/TMEM150_Autophagy_Mod	Family

Pfam: PF10277

Catalyzed reactions (Rhea), 4 shown:

• K(+)(in) = K(+)(out) (RHEA:29463)
• Ca(2+)(in) = Ca(2+)(out) (RHEA:29671)
• Mg(2+)(in) = Mg(2+)(out) (RHEA:29827)
• Na(+)(in) = Na(+)(out) (RHEA:34963)

UniProt features (17 total): topological domain 7, transmembrane region 6, sequence conflict 2, chain 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-B9EJG8-F1	91.57	0.83

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 89 (showing top): GSE18804_SPLEEN_MACROPHAGE_VS_COLON_TUMORAL_MACROPHAGE_UP, GOCC_VACUOLAR_MEMBRANE, GOBP_CELLULAR_RESPONSE_TO_EXTERNAL_STIMULUS, GOZGIT_ESR1_TARGETS_DN, CHANDRAN_METASTASIS_DN, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_RESPONSE_TO_ABIOTIC_STIMULUS, CHIANG_LIVER_CANCER_SUBCLASS_INTERFERON_DN, GOBP_SENSORY_PERCEPTION, GOBP_NEUROMUSCULAR_PROCESS_CONTROLLING_POSTURE, CUI_TCF21_TARGETS_2_DN, GOBP_NEUROMUSCULAR_PROCESS, GOBP_TRANSMEMBRANE_TRANSPORT, GOBP_RESPONSE_TO_MECHANICAL_STIMULUS, CHIANG_LIVER_CANCER_SUBCLASS_CTNNB1_UP

GO Biological Process (3): proprioception (GO:0019230), cellular response to mechanical stimulus (GO:0071260), monoatomic cation transmembrane transport (GO:0098655)

GO Molecular Function (1): mechanosensitive monoatomic cation channel activity (GO:0140135)

GO Cellular Component (5): lysosomal membrane (GO:0005765), plasma membrane (GO:0005886), lysosome (GO:0005764), endomembrane system (GO:0012505), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
sensory perception	1
neuromuscular process controlling posture	1
response to mechanical stimulus	1
cellular response to abiotic stimulus	1
cellular response to external stimulus	1
monoatomic cation transport	1
monoatomic ion transmembrane transport	1
monoatomic cation channel activity	1
mechanosensitive monoatomic ion channel activity	1
lysosome	1
lytic vacuole membrane	1
membrane	1
cell periphery	1
lytic vacuole	1
vacuole	1
plasma membrane	1

Protein interactions and networks

STRING

400 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TMEM150C	PIEZO1	Q92508	667
TMEM150C	PIEZO2	Q9H5I5	623
TMEM150C	TMEM120A	Q9BXJ8	542
TMEM150C	TMEM221	A6NGB7	484
TMEM150C	PHACTR2	O75167	472
TMEM150C	GPRC5C	Q9NQ84	447
TMEM150C	KCNK2	O95069	435
TMEM150C	TRPV4	Q9HBA0	425
TMEM150C	LHFPL7	Q6ICI0	420
TMEM150C	TMEM87A	Q8NBN3	413
TMEM150C	GPR137C	Q8N3F9	410
TMEM150C	TMEM63C	Q9P1W3	399
TMEM150C	OVOL3	O00110	390
TMEM150C	TMEM132E	Q6IEE7	387
TMEM150C	ASIC2	Q16515	378

IntAct

0 interactions, top by confidence:

BioGRID (15): TMEM150C (Affinity Capture-RNA), TMEM150C (Positive Genetic), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), TMEM150C (Co-fractionation), USP39 (Co-fractionation)

ESM2 similar proteins: A2AE42, A3A9H6, A5D7C9, A5D9A7, A6NM10, B3SHH9, B5DFH9, B9EJG8, F1NZP5, O14569, P10897, P49447, P82352, Q08DE1, Q14714, Q148G2, Q3ZCD2, Q5E965, Q5ND56, Q5RCZ2, Q5U2W7, Q5ZJX0, Q60720, Q62147, Q641Y1, Q6GPL4, Q6P0C6, Q6P1H1, Q71RH2, Q7TNV1, Q80ZE4, Q86TG1, Q8BMD6, Q8C8S3, Q8IXF9, Q8N8Q1, Q8NBI2, Q8TBR7, Q8VHW3, Q8VHW7

Diamond homologs: A5D7C9, A6NC51, A9JSP6, B5DFH9, B9EJG8, Q28BP2, Q32PK2, Q3ZC48, Q4V7T3, Q4V7T7, Q6UX65, Q8C8S3, Q8R218, A7MBB3, Q5BK09, Q6GPL4, Q86TG1, Q91WN2, Q9CR48, Q9DC58, Q9QZE9, Q8N682, Q5EAK8, Q6NRS6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

29 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	23
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1836 predictions. Top by Δscore:

Variant	Effect	Δscore
4:82502793:TG:T	acceptor_gain	1.0000
4:82502796:T:C	acceptor_gain	1.0000
4:82502796:T:TC	acceptor_gain	1.0000
4:82504570:ATACT:A	donor_loss	1.0000
4:82504571:TAC:T	donor_loss	1.0000
4:82504572:AC:A	donor_loss	1.0000
4:82504573:CTCA:C	donor_loss	1.0000
4:82504575:C:CC	donor_loss	1.0000
4:82504576:A:AC	donor_gain	1.0000
4:82504577:C:CC	donor_gain	1.0000
4:82504577:CA:C	donor_gain	1.0000
4:82504577:CACT:C	donor_gain	1.0000
4:82504663:TACCA:T	acceptor_gain	1.0000
4:82504664:ACCA:A	acceptor_gain	1.0000
4:82504665:CCA:C	acceptor_gain	1.0000
4:82504665:CCAC:C	acceptor_gain	1.0000
4:82504666:CA:C	acceptor_gain	1.0000
4:82504666:CAC:C	acceptor_gain	1.0000
4:82504668:C:CC	acceptor_gain	1.0000
4:82504668:CTGAA:C	acceptor_loss	1.0000
4:82490118:T:A	donor_gain	0.9900
4:82490240:T:C	acceptor_gain	0.9900
4:82495752:AAC:A	donor_gain	0.9900
4:82502722:CTTA:C	donor_loss	0.9900
4:82502724:TACCT:T	donor_loss	0.9900
4:82502790:CAATG:C	acceptor_gain	0.9900
4:82502792:ATG:A	acceptor_gain	0.9900
4:82502795:C:CC	acceptor_gain	0.9900
4:82502926:TC:T	acceptor_gain	0.9900
4:82502927:CC:C	acceptor_gain	0.9900

AlphaMissense

1627 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
4:82496071:A:C	F120L	1.000
4:82496071:A:T	F120L	1.000
4:82496073:A:G	F120L	1.000
4:82496093:C:T	G113E	1.000
4:82485667:C:A	W198C	0.999
4:82485667:C:G	W198C	0.999
4:82485669:A:G	W198R	0.999
4:82485669:A:T	W198R	0.999
4:82490169:A:G	W145R	0.999
4:82490169:A:T	W145R	0.999
4:82490183:C:T	G140D	0.999
4:82490207:C:A	G132V	0.999
4:82490207:C:T	G132E	0.999
4:82490208:C:G	G132R	0.999
4:82490208:C:T	G132R	0.999
4:82490217:G:C	H129D	0.999
4:82496074:A:C	N119K	0.999
4:82496074:A:T	N119K	0.999
4:82496078:C:A	G118V	0.999
4:82496078:C:T	G118D	0.999
4:82496079:C:G	G118R	0.999
4:82496093:C:A	G113V	0.999
4:82496094:C:G	G113R	0.999
4:82496094:C:T	G113R	0.999
4:82496174:C:G	R86P	0.999
4:82502743:G:C	N73K	0.999
4:82502743:G:T	N73K	0.999
4:82502758:A:C	F68L	0.999
4:82502758:A:T	F68L	0.999
4:82502760:A:G	F68L	0.999

dbSNP variants (sampled 300 via entrez): RS1000029670 (4:82528846 G>A), RS1000044853 (4:82523290 A>G), RS1000065033 (4:82530295 A>C,T), RS10001408 (4:82507270 T>C), RS1000160956 (4:82535679 G>C), RS1000174255 (4:82492132 G>C,T), RS1000229580 (4:82499655 G>A), RS10002527 (4:82508700 A>T), RS1000274159 (4:82542021 C>T), RS1000285945 (4:82492113 G>A,T), RS1000294917 (4:82498985 CTT>C), RS1000328805 (4:82549603 A>G), RS1000346980 (4:82499332 G>T), RS1000358140 (4:82529074 G>A), RS1000375933 (4:82542954 A>G)

Disease associations

OMIM: gene MIM:617292 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

28 total (human), top 28 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	increases expression	3
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
S-(1,2-dichlorovinyl)cysteine	affects cotreatment, decreases expression, affects response to substance, increases expression	1
mercuric bromide	decreases expression, affects cotreatment	1
perfluoro-n-nonanoic acid	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	decreases expression, affects cotreatment	1
abrine	decreases expression	1
Grape Seed Proanthocyanidins	affects cotreatment, decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
Acetaminophen	increases expression	1
Vehicle Emissions	decreases expression	1
Benzo(a)pyrene	affects methylation, increases methylation	1
Cadmium	decreases expression, increases abundance	1
Catechin	decreases expression, affects cotreatment	1
Coumestrol	decreases expression	1
Diethylhexyl Phthalate	decreases expression	1
Dimethyl Sulfoxide	affects expression	1
Estradiol	decreases expression	1
Fluorouracil	decreases expression	1
Lipopolysaccharides	affects cotreatment, decreases expression, affects response to substance, increases expression	1
Silicon Dioxide	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Tretinoin	increases expression	1
Triclosan	decreases expression	1
Aflatoxin B1	increases methylation	1
Cadmium Chloride	decreases expression, increases abundance	1
Okadaic Acid	decreases expression	1
Particulate Matter	affects expression	1

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_TS79	HAP1 TMEM150C (-) 1	Cancer cell line	Male
CVCL_TS80	HAP1 TMEM150C (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.