TMEM35B

Summary

TMEM35B (transmembrane protein 35B, HGNC:40021) is a protein-coding gene on chromosome 1p34.3, encoding Transmembrane protein 35B (Q8NCS4).

Predicted to be located in membrane.

Source: NCBI Gene 100506144 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 1 total
• MANE Select transcript: NM_001195156

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:40021
Approved symbol	TMEM35B
Name	transmembrane protein 35B
Location	1p34.3
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000243749
Ensembl biotype	protein_coding
Entrez	100506144

Gene structure

Transcript identifiers

Ensembl transcripts: 2 — 2 protein_coding

ENST00000373337, ENST00000911434

RefSeq mRNA: 1 — MANE Select: NM_001195156 NM_001195156

CCDS: CCDS53296

Canonical transcript exons

ENST00000373337 — 3 exons

Exon	Start	End
ENSE00003498501	34983767	34983947
ENSE00003978298	34981380	34982119
ENSE00003978299	34985198	34985347

Expression profiles

Bgee: expression breadth ubiquitous, 133 present calls, max score 94.86.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 16.1685 / max 132.9592, expressed in 1794 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
11657	16.1685	1794

Top tissues by expression

133 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
placenta	UBERON:0001987	94.86	gold quality
gall bladder	UBERON:0002110	94.80	gold quality
right lung	UBERON:0002167	94.59	gold quality
omental fat pad	UBERON:0010414	93.84	gold quality
fallopian tube	UBERON:0003889	93.70	gold quality
right coronary artery	UBERON:0001625	93.56	gold quality
left coronary artery	UBERON:0001626	93.30	gold quality
adipose tissue	UBERON:0001013	93.09	gold quality
lymph node	UBERON:0000029	92.84	gold quality
smooth muscle tissue	UBERON:0001135	92.82	gold quality
body of uterus	UBERON:0009853	92.82	gold quality
ascending aorta	UBERON:0001496	92.81	gold quality
thoracic aorta	UBERON:0001515	92.81	gold quality
right lobe of thyroid gland	UBERON:0001119	92.71	gold quality
left lobe of thyroid gland	UBERON:0001120	92.68	gold quality
descending thoracic aorta	UBERON:0002345	92.68	gold quality
endocervix	UBERON:0000458	92.67	gold quality
upper lobe of left lung	UBERON:0008952	92.66	gold quality
left uterine tube	UBERON:0001303	92.55	gold quality
stromal cell of endometrium	CL:0002255	92.48	gold quality
subcutaneous adipose tissue	UBERON:0002190	92.46	gold quality
tibial nerve	UBERON:0001323	92.42	gold quality
granulocyte	CL:0000094	92.24	gold quality
thyroid gland	UBERON:0002046	92.21	gold quality
islet of Langerhans	UBERON:0000006	91.96	gold quality
myometrium	UBERON:0001296	91.96	gold quality
right adrenal gland	UBERON:0001233	91.85	gold quality
popliteal artery	UBERON:0002250	91.58	gold quality
tibial artery	UBERON:0007610	91.58	gold quality
left adrenal gland	UBERON:0001234	91.56	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	22.84
E-MTAB-7303	no	450.18

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

36 targeting TMEM35B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-12118	100.00	65.88	1270
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-3173-3P	99.98	66.49	1217
HSA-MIR-6891-5P	99.98	66.53	1372
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-548AN	99.97	70.91	2817
HSA-MIR-9-3P	99.96	70.88	2068
HSA-MIR-335-3P	99.93	73.36	4958
HSA-MIR-1-3P	99.93	72.35	1914
HSA-MIR-206	99.93	72.50	1893
HSA-MIR-613	99.91	71.50	1710
HSA-MIR-124-3P	99.89	73.74	3043
HSA-MIR-506-3P	99.89	73.55	3057
HSA-MIR-548AZ-5P	99.83	69.94	3230
HSA-MIR-548T-5P	99.83	69.91	3220
HSA-MIR-12124	99.68	69.17	2700
HSA-MIR-586	99.65	70.40	2051
HSA-MIR-2053	99.57	69.15	1635
HSA-MIR-5007-3P	99.51	68.14	1242
HSA-MIR-766-5P	99.47	67.91	2225
HSA-MIR-140-5P	99.44	67.20	792
HSA-MIR-513A-3P	99.39	70.63	3620
HSA-MIR-513C-3P	99.39	70.63	3620
HSA-MIR-4311	99.31	70.47	3041
HSA-MIR-329-5P	99.27	68.11	1597
HSA-MIR-5582-5P	99.27	71.42	1879
HSA-MIR-4711-5P	98.89	68.00	965

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
mus_musculus	Tmem35b	ENSMUSG00000070737
rattus_norvegicus	Tmem35b	ENSRNOG00000028083
drosophila_melanogaster	CG13920	FBGN0025712

Paralogs (1): TMEM35A (ENSG00000126950)

Protein

Protein identifiers

Transmembrane protein 35B — Q8NCS4 (reviewed: Q8NCS4)

Alternative names: ZMYM6 neighbor protein

All UniProt accessions (1): Q8NCS4

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Membrane.

Similarity. Belongs to the DoxX family.

RefSeq proteins (1): NP_001182085* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR032808	DoxX	Family
IPR040399	TMEM35A/B	Family

Pfam: PF07681

UniProt features (5 total): transmembrane region 3, signal peptide 1, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8NCS4-F1	78.18	0.36

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 59 (showing top): chr1p34, CASORELLI_APL_SECONDARY_VS_DE_NOVO_UP, SCHAEFFER_PROSTATE_DEVELOPMENT_48HR_UP, CHARAFE_BREAST_CANCER_LUMINAL_VS_BASAL_DN, LASTOWSKA_NEUROBLASTOMA_COPY_NUMBER_DN, ALCALA_APOPTOSIS, RAY_TUMORIGENESIS_BY_ERBB2_CDC25A_DN, LEE_BMP2_TARGETS_UP, ZNF2_TARGET_GENES, ZNF423_TARGET_GENES, ZNF92_TARGET_GENES, MIR5696, MIR506_3P, MIR124_3P, MIR4311

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (1): membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	1

Protein interactions and networks

STRING

98 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TMEM35B	GPN2	Q9H9Y4	568
TMEM35B	TOMM20L	Q6UXN7	527
TMEM35B	TMEM35A	Q53FP2	480
TMEM35B	CCDC18	Q5T9S5	478
TMEM35B	DDX42	Q86XP3	466
TMEM35B	ZMYM4	Q5VZL5	449
TMEM35B	ZMYM6	O95789	448
TMEM35B	UROS	P10746	442
TMEM35B	ZMYM1	Q5SVZ6	396
TMEM35B	RIC3	Q7Z5B4	372
TMEM35B	KIAA0586	Q9BVV6	371
TMEM35B	TIMM9	Q9Y5J7	367
TMEM35B	ZNF732	B4DXR9	348
TMEM35B	ARID4A	P29374	336
TMEM35B	FNBP1L	Q5T0N5	321

IntAct

5 interactions, top by confidence:

A	B	Type	Score
TMEM35B	TRIM13	psi-mi:“MI:0914”(association)	0.350
TCTN2	TMEM120B	psi-mi:“MI:2364”(proximity)	0.270
TCTN3	TMEM120B	psi-mi:“MI:2364”(proximity)	0.270

BioGRID (25): ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), ZMYM6NB (Proximity Label-MS), AIM1 (Affinity Capture-MS), HIST2H2AC (Affinity Capture-MS), VSIG8 (Affinity Capture-MS), TRIM13 (Affinity Capture-MS)

ESM2 similar proteins: A2AWR3, A4IGI5, D3ZYP5, O18405, O88496, P38435, P52650, P58355, Q07175, Q0P4Y8, Q18864, Q28FA9, Q3KQE5, Q3KTM2, Q3U0Y2, Q4R5F4, Q4V3B8, Q4V8K1, Q53FP2, Q53P98, Q58EL2, Q5E9T5, Q5R9A7, Q5RCJ4, Q5RF50, Q68FV1, Q6GN30, Q6JAM9, Q6P2T0, Q6P8F8, Q6ZPD9, Q6ZQE4, Q71B07, Q7Z3F1, Q7ZVP8, Q7ZWN0, Q7ZY07, Q8NCS4, Q923B6, Q95L73

Diamond homologs: D3ZYP5, Q3U0Y2, Q8NCS4

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

1 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

462 predictions. Top by Δscore:

Variant	Effect	Δscore
1:34983795:A:AC	donor_gain	1.0000
1:34983796:C:CC	donor_gain	1.0000
1:34983796:CTGAT:C	donor_gain	1.0000
1:34983800:T:C	donor_gain	1.0000
1:34983944:CATT:C	acceptor_gain	1.0000
1:34983766:CCCAT:C	donor_gain	0.9900
1:34983789:CAAG:C	donor_gain	0.9900
1:34983792:G:C	donor_gain	0.9900
1:34983948:C:CC	acceptor_gain	0.9900
1:34985192:GCTCA:G	donor_loss	0.9900
1:34985193:CTCAC:C	donor_loss	0.9900
1:34985194:TCA:T	donor_loss	0.9900
1:34985195:CACCA:C	donor_loss	0.9900
1:34985196:A:AC	donor_gain	0.9900
1:34985196:A:T	donor_loss	0.9900
1:34985196:ACCAT:A	donor_gain	0.9900
1:34985197:C:CC	donor_gain	0.9900
1:34985197:C:CT	donor_loss	0.9900
1:34985197:CCAT:C	donor_gain	0.9900
1:34985197:CCATC:C	donor_gain	0.9900
1:34982117:CCC:C	acceptor_gain	0.9800
1:34982118:CCC:C	acceptor_gain	0.9800
1:34983765:AC:A	donor_gain	0.9800
1:34983766:CC:C	donor_gain	0.9800
1:34983943:GCATT:G	acceptor_gain	0.9800
1:34983944:CATTC:C	acceptor_gain	0.9800
1:34983945:ATT:A	acceptor_gain	0.9800
1:34983946:TT:T	acceptor_gain	0.9800
1:34983946:TTCTA:T	acceptor_loss	0.9800
1:34983947:TC:T	acceptor_loss	0.9800

AlphaMissense

975 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:34983927:A:C	F43L	0.978
1:34983927:A:T	F43L	0.978
1:34983929:A:G	F43L	0.978
1:34983936:G:C	F40L	0.978
1:34983936:G:T	F40L	0.978
1:34983938:A:G	F40L	0.978
1:34983795:A:C	S87R	0.976
1:34983795:A:T	S87R	0.976
1:34983797:T:G	S87R	0.976
1:34982057:A:G	C118R	0.964
1:34985258:G:C	F16L	0.961
1:34985258:G:T	F16L	0.961
1:34985260:A:G	F16L	0.961
1:34983937:A:C	F40C	0.952
1:34983849:T:A	E69D	0.950
1:34983849:T:G	E69D	0.950
1:34983838:C:T	G73E	0.949
1:34983859:C:T	G66D	0.949
1:34985248:C:G	G20R	0.945
1:34985248:C:T	G20R	0.945
1:34985269:C:G	G13R	0.945
1:34985268:C:T	G13D	0.944
1:34983784:A:G	L91S	0.941
1:34983767:C:G	G97R	0.940
1:34983767:C:T	G97R	0.940
1:34983928:A:C	F43C	0.939
1:34983860:C:G	G66R	0.938
1:34983839:C:G	G73R	0.937
1:34983839:C:T	G73R	0.937
1:34983839:C:A	G73W	0.931

dbSNP variants (sampled 300 via entrez): RS1000208212 (1:34986780 T>G), RS1000276884 (1:34986395 T>C), RS1001491857 (1:34983384 C>G,T), RS1001754405 (1:34983074 C>A), RS1002134267 (1:34984611 C>A,G), RS1002360341 (1:34981530 T>C), RS1002779140 (1:34981686 C>A,T), RS1002811946 (1:34981415 A>C,G), RS1003025803 (1:34984265 G>A,T), RS1003575878 (1:34984553 A>C,G), RS1004577772 (1:34982899 C>T), RS1005109255 (1:34985475 G>T), RS1005205296 (1:34982698 A>G), RS1005341287 (1:34982963 G>A), RS1005786197 (1:34986132 T>A)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST004751_3	Serum uric acid levels in response to allopurinol in gout	4.000000e-07

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004761	uric acid measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

16 total (human), top 16 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Smoke	decreases expression, increases abundance	2
Valproic Acid	affects expression, decreases expression	2
Cadmium Chloride	decreases expression, increases abundance	2
trichostatin A	decreases expression	1
perfluorooctane sulfonic acid	increases expression	1
Decitabine	affects expression	1
Sunitinib	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1
Cadmium	decreases expression, increases abundance	1
Cisplatin	affects expression	1
Copper	increases expression, affects binding	1
Disulfiram	affects binding, increases expression	1
Zinc	decreases expression	1
Aflatoxin B1	increases expression	1
Antirheumatic Agents	decreases expression	1
Okadaic Acid	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.