TOMM34

Summary

TOMM34 (translocase of outer mitochondrial membrane 34, HGNC:15746) is a protein-coding gene on chromosome 20q13.12, encoding Mitochondrial import receptor subunit TOM34 (Q15785). Plays a role in the import of cytosolically synthesized preproteins into mitochondria.

The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats.

Source: NCBI Gene 10953 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 34 total
• Druggable target: yes
• MANE Select transcript: NM_006809

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:15746
Approved symbol	TOMM34
Name	translocase of outer mitochondrial membrane 34
Location	20q13.12
Locus type	gene with protein product
Status	Approved
Aliases	TOM34, HTOM34P
Ensembl gene	ENSG00000025772
Ensembl biotype	protein_coding
OMIM	616049
Entrez	10953

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 5 protein_coding

ENST00000372813, ENST00000879562, ENST00000879563, ENST00000912595, ENST00000912596

RefSeq mRNA: 1 — MANE Select: NM_006809 NM_006809

CCDS: CCDS13340

Canonical transcript exons

ENST00000372813 — 7 exons

Exon	Start	End
ENSE00000662317	44943453	44943579
ENSE00000662318	44948730	44948877
ENSE00000662319	44951833	44952002
ENSE00000662322	44955068	44955220
ENSE00000662323	44956386	44956485
ENSE00000844908	44942130	44943213
ENSE00000844909	44960207	44960397

Expression profiles

Bgee: expression breadth ubiquitous, 270 present calls, max score 98.28.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 26.3304 / max 237.3211, expressed in 1813 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
187370	26.3304	1813

Top tissues by expression

287 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right testis	UBERON:0004534	98.28	gold quality
left testis	UBERON:0004533	98.25	gold quality
testis	UBERON:0000473	96.87	gold quality
adrenal tissue	UBERON:0018303	95.56	gold quality
islet of Langerhans	UBERON:0000006	95.44	gold quality
nucleus accumbens	UBERON:0001882	95.09	gold quality
right uterine tube	UBERON:0001302	94.61	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	93.97	gold quality
right adrenal gland cortex	UBERON:0035827	93.96	gold quality
caudate nucleus	UBERON:0001873	93.85	gold quality
right adrenal gland	UBERON:0001233	93.69	gold quality
left adrenal gland	UBERON:0001234	92.97	gold quality
putamen	UBERON:0001874	92.77	gold quality
left adrenal gland cortex	UBERON:0035825	92.64	gold quality
adrenal gland	UBERON:0002369	92.35	gold quality
adrenal cortex	UBERON:0001235	92.23	gold quality
cingulate cortex	UBERON:0003027	91.81	gold quality
anterior cingulate cortex	UBERON:0009835	91.78	gold quality
ileal mucosa	UBERON:0000331	91.69	gold quality
prefrontal cortex	UBERON:0000451	91.29	gold quality
Brodmann (1909) area 9	UBERON:0013540	91.08	gold quality
right frontal lobe	UBERON:0002810	90.99	gold quality
adenohypophysis	UBERON:0002196	90.48	gold quality
Ammon’s horn	UBERON:0001954	90.31	gold quality
amygdala	UBERON:0001876	90.30	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	89.88	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	89.77	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	89.74	gold quality
telencephalon	UBERON:0001893	89.35	gold quality
frontal cortex	UBERON:0001870	89.17	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	6.88

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): NFE2L2, NRF1

miRNA regulators (miRDB)

49 targeting TOMM34, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4283	100.00	66.42	2097
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-1184	99.99	68.19	1458
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-3065-5P	99.97	71.56	3281
HSA-MIR-548AN	99.97	70.91	2817
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-3910	99.95	71.13	2227
HSA-MIR-185-3P	99.95	67.01	1743
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-4731-5P	99.89	67.23	2537
HSA-MIR-124-3P	99.89	73.74	3043
HSA-MIR-506-3P	99.89	73.55	3057
HSA-MIR-605-3P	99.88	69.22	1833
HSA-MIR-4779	99.86	66.50	1583
HSA-MIR-3133	99.81	70.92	3506
HSA-MIR-6752-3P	99.72	66.71	1587
HSA-MIR-1296-3P	99.72	64.04	636
HSA-MIR-6762-3P	99.66	66.94	1188
HSA-MIR-1251-3P	99.64	67.21	1408
HSA-MIR-1260A	99.61	66.67	1098
HSA-MIR-1260B	99.61	66.67	1098
HSA-MIR-7159-5P	99.53	72.12	2472
HSA-MIR-4489	99.50	65.56	785
HSA-MIR-3191-5P	99.24	66.52	1722
HSA-MIR-6815-3P	99.13	68.98	1530
HSA-MIR-625-5P	99.02	68.64	2031

Literature-anchored findings (GeneRIF, showing 15)

• Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins (PMID:11913975)
• Yeast two-hybrid screening identifies binding partners of human Tom34 that have ATPase activity and form a complex with Tom34 in the cytosol (PMID:11913976)
• TOMM34 is involved in the growth of colon cancer cells (PMID:16820880)
• Data show that NRF-1 is the main transcription factor regulating the expression of TOMM34, and that Sp1 interacts with NRF-1 to stimulate the promoter’s full activity. (PMID:18364745)
• Results identify Tom34 as a cochaperone of Hsp70/Hsp90 in mitochondrial protein import. (PMID:22178133)
• TOMM34 expression in early invasive breast cancer: a biomarker associated with poor outcome. (PMID:23053644)
• These results suggest that Tomm34 represents a novel scaffolding co-chaperone of Hsp70 and Hsp90, which may facilitate Hsp70/Hsp90 cooperation during protein folding (PMID:24567332)
• Data show that Tomm34 extensively interacts with the C-terminal structures of Hsp70 selectively in its ATP-bound state and that this interaction is strongly entropically driven. (PMID:26944342)
• Expression of TOMM34 and Its Clinicopathological Correlations in Urothelial Carcinoma of the Bladder. (PMID:30382527)
• TOM34 and its partner HSP90AA1 might be used as a potential biomarker for monitoring HCV-induced hepatocellular carcinoma progression in the Egyptian population. Future large-scale validation studies are warranted. (PMID:30521791)
• Translocase of outer mitochondrial membrane 34 (Tomm34) is commonly expressed at high levels in epithelial ovarian cancers, except for the clear cell and mucinous subtypes. The expression of Tomm34 is positively correlated with FIGO stage. Tomm34 levels also indicates poor prognosis and progression for patients with mutant p53. (PMID:30917858)
• The interaction of the mitochondrial protein importer TOMM34 with HSP70 is regulated by TOMM34 phosphorylation and binding to 14-3-3 adaptors. (PMID:32371396)
• High Expression of Tomm34 and Its Correlations With Clinicopathology in Oral Squamous Cell Carcinoma. (PMID:34257607)
• TOMM34 promotes cell proliferation, migration, and invasion of OSCC and modulates mitochondrial function. (PMID:36367442)
• Role of TOMM34 on NF-kappaB activation-related hyperinflammation in severely ill patients with COVID-19 and influenza. (PMID:39276680)

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
mus_musculus	Tomm34	ENSMUSG00000018322
drosophila_melanogaster	Stip1	FBGN0024352
drosophila_melanogaster	Sgt	FBGN0032640
drosophila_melanogaster	unc-45	FBGN0288846
caenorhabditis_elegans		WBGENE00006781
caenorhabditis_elegans		WBGENE00019983

Paralogs (18): RPAP3 (ENSG00000005175), ST13 (ENSG00000100380), STUB1 (ENSG00000103266), SPAG1 (ENSG00000104450), SGTA (ENSG00000104969), TTC1 (ENSG00000113312), TTC31 (ENSG00000115282), UNC45A (ENSG00000140553), UNC45B (ENSG00000141161), SPATA16 (ENSG00000144962), TTC12 (ENSG00000149292), TOMM70 (ENSG00000154174), SUGT1 (ENSG00000165416), STIP1 (ENSG00000168439), TTC32 (ENSG00000183891), SGTB (ENSG00000197860), TTC4 (ENSG00000243725), DNAAF4 (ENSG00000256061)

Protein

Protein identifiers

Mitochondrial import receptor subunit TOM34 — Q15785 (reviewed: Q15785)

Alternative names: Translocase of outer membrane 34 kDa subunit

All UniProt accessions (1): Q15785

UniProt curated annotations — full annotation on UniProt →

Function. Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.

Subunit / interactions. Interacts with HSP90A, VCP, ATP6V1D, KIAA0665, AMPK, and DMAP1 through its TPR repeat.

Subcellular location. Cytoplasm. Mitochondrion outer membrane.

Tissue specificity. Ubiquitous.

Similarity. Belongs to the Tom34 family.

RefSeq proteins (1): NP_006800* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR011990	TPR-like_helical_dom_sf	Homologous_superfamily
IPR019734	TPR_rpt	Repeat
IPR051982	CiliaryAsmbly_MitoImport	Family

Pfam: PF00515

UniProt features (18 total): repeat 6, sequence conflict 4, modified residue 3, chain 1, cross-link 1, sequence variant 1, region of interest 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q15785-F1	87.93	0.75

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (4): 160, 186, 197, 8

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 155 (showing top): STARK_PREFRONTAL_CORTEX_22Q11_DELETION_DN, GOBP_PROTEIN_TARGETING, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ORGANELLE, GTGCCTT_MIR506, WEI_MYCN_TARGETS_WITH_E_BOX, MODULE_206, GOCC_MITOCHONDRIAL_ENVELOPE, FLECHNER_BIOPSY_KIDNEY_TRANSPLANT_REJECTED_VS_OK_DN, DOUGLAS_BMI1_TARGETS_UP, HU_GENOTOXIN_ACTION_DIRECT_VS_INDIRECT_24HR, GOBP_PROTEIN_TARGETING_TO_MITOCHONDRION, MODULE_18, RASHI_RESPONSE_TO_IONIZING_RADIATION_5, MORF_ORC1L, MARSON_BOUND_BY_FOXP3_STIMULATED

GO Biological Process (1): obsolete protein targeting to mitochondrion (GO:0006626)

GO Molecular Function (2): heat shock protein binding (GO:0031072), protein binding (GO:0005515)

GO Cellular Component (6): nucleus (GO:0005634), mitochondrion (GO:0005739), mitochondrial outer membrane (GO:0005741), cytosol (GO:0005829), membrane (GO:0016020), cytoplasm (GO:0005737)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
intracellular membrane-bounded organelle	2
cytoplasm	2
protein binding	1
binding	1
mitochondrial membrane	1
organelle outer membrane	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

1901 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TOMM34	HSP90AA1	P07900	706
TOMM34	HSP90AB1	P08238	692
TOMM34	HSPA4	P34932	682
TOMM34	RNF43	Q68DV7	606
TOMM34	TOMM40	O96008	516
TOMM34	TOMM5	Q8N4H5	486
TOMM34	TIMM17A	Q99595	476
TOMM34	FKBP2	P26885	465
TOMM34	TIMM17B	O60830	464
TOMM34	TOMM6	Q96B49	462
TOMM34	YME1L1	Q96TA2	444
TOMM34	FKBP6	O75344	442
TOMM34	TOMM22	Q9NS69	440
TOMM34	TIMM22	Q9Y584	438
TOMM34	IGF2BP3	O00425	419

IntAct

78 interactions, top by confidence:

A	B	Type	Score
MED17	MED19	psi-mi:“MI:0914”(association)	0.840
HSP90AA1	CHUK	psi-mi:“MI:0914”(association)	0.670
SNRNP27	UBA6	psi-mi:“MI:0914”(association)	0.530
GPR21	TMEM120B	psi-mi:“MI:0914”(association)	0.530
AKIRIN2	RGPD3	psi-mi:“MI:0914”(association)	0.530
COL10A1	C1QL1	psi-mi:“MI:0914”(association)	0.530
USP47	DENR	psi-mi:“MI:0914”(association)	0.530
SLC16A11	H2AB2	psi-mi:“MI:0914”(association)	0.530
HSP90AA1	USP19	psi-mi:“MI:0914”(association)	0.530
FUOM	EEF1A2	psi-mi:“MI:0914”(association)	0.530
TIMMDC1	NDUFS8	psi-mi:“MI:0914”(association)	0.530
TOMM34	TINF2	psi-mi:“MI:0915”(physical association)	0.510
ATOSB	TOMM34	psi-mi:“MI:0915”(physical association)	0.400
TOMM34	ACTB	psi-mi:“MI:0915”(physical association)	0.400
TERF1	TOMM34	psi-mi:“MI:0915”(physical association)	0.370
TOMM34	ACD	psi-mi:“MI:0915”(physical association)	0.370
TOMM34	POT1	psi-mi:“MI:0915”(physical association)	0.370
GTF2E2	UBA6	psi-mi:“MI:0914”(association)	0.350
ZNF490	NEMF	psi-mi:“MI:0914”(association)	0.350
CCDC82	LY6G6C	psi-mi:“MI:0914”(association)	0.350
MEPE	FN1	psi-mi:“MI:0914”(association)	0.350
FUOM	PRRC2A	psi-mi:“MI:0914”(association)	0.350
SH3BGRL	MYO1C	psi-mi:“MI:0914”(association)	0.350
HSP90AA1	URI1	psi-mi:“MI:0914”(association)	0.350
HLA-A	AIP	psi-mi:“MI:0914”(association)	0.350
	PSMD3	psi-mi:“MI:0914”(association)	0.350
	SDCBP	psi-mi:“MI:0914”(association)	0.350

BioGRID (149): TOMM34 (Affinity Capture-RNA), TOMM34 (Affinity Capture-RNA), DNAJC7 (Co-fractionation), FUS (Co-fractionation), HNRNPD (Co-fractionation), TARS (Co-fractionation), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS), TOMM34 (Affinity Capture-MS)

ESM2 similar proteins: A0A0P0VG31, A0A2H5Q1B8, A4K2V0, A8XHX1, F1RBN2, O14085, O14217, P19878, P27124, P30416, Q02790, Q06AN9, Q07617, Q13217, Q13451, Q15785, Q20683, Q27968, Q32NU8, Q3KRD5, Q3ZBR5, Q5JNB5, Q5RF88, Q5U2X2, Q5ZI13, Q5ZKQ3, Q64378, Q68FQ7, Q6AZT2, Q6ES52, Q6NU95, Q7XJS0, Q7ZU45, Q80ZX8, Q91YW3, Q91Z38, Q95L05, Q99614, Q9CYG7, Q9D706

Diamond homologs: A4K2V0, A6HD62, A6ZRW3, D7REX8, F1RBN2, F4IRM4, F4JTI1, F4K487, F4KCL7, O13754, O14217, O16259, O35814, O48802, O54981, O94826, O95801, P07213, P23231, P25638, P31948, P33313, P38825, P53041, P53042, Q07617, Q12118, Q13451, Q15785, Q32PZ3, Q3KRD5, Q3ZBR5, Q43207, Q4R8N7, Q5EA11, Q5PPS5, Q5R8D8, Q5RAP0, Q5U2X2, Q5VJS5

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 105 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

GO biological processes:

GO term	Partners	Fold	FDR
telomere maintenance via telomerase	5	38.2×	1e-04
protein folding	9	9.7×	1e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

34 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	25
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

845 predictions. Top by Δscore:

Variant	Effect	Δscore
20:44943447:TTTTA:T	donor_loss	1.0000
20:44943448:TTTA:T	donor_loss	1.0000
20:44943449:TTACC:T	donor_loss	1.0000
20:44943450:TA:T	donor_loss	1.0000
20:44943452:C:CG	donor_loss	1.0000
20:44943575:GTGCT:G	acceptor_gain	1.0000
20:44943576:TGCT:T	acceptor_gain	1.0000
20:44943578:CT:C	acceptor_gain	1.0000
20:44943580:C:CC	acceptor_gain	1.0000
20:44943580:C:CG	acceptor_loss	1.0000
20:44955063:CTCA:C	donor_loss	1.0000
20:44955064:TCA:T	donor_loss	1.0000
20:44955066:A:AC	donor_gain	1.0000
20:44955067:C:CC	donor_gain	1.0000
20:44955067:C:CT	donor_loss	1.0000
20:44955219:CT:C	acceptor_gain	1.0000
20:44955221:C:CC	acceptor_gain	1.0000
20:44956384:A:AC	donor_gain	1.0000
20:44956385:C:CT	donor_gain	1.0000
20:44956385:CG:C	donor_gain	1.0000
20:44956385:CGA:C	donor_gain	1.0000
20:44956385:CGAA:C	donor_gain	1.0000
20:44960201:TCGTA:T	donor_loss	1.0000
20:44960202:CGTA:C	donor_loss	1.0000
20:44960203:GTA:G	donor_loss	1.0000
20:44960204:TA:T	donor_loss	1.0000
20:44960205:ACCTT:A	donor_loss	1.0000
20:44960206:C:CT	donor_loss	1.0000
20:44943209:TAGTC:T	acceptor_gain	0.9900
20:44943211:GTCC:G	acceptor_loss	0.9900

AlphaMissense

2009 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
20:44943476:C:G	A268P	0.999
20:44943478:C:G	R267P	0.999
20:44943470:C:G	A270P	0.998
20:44943480:T:A	R266S	0.998
20:44943480:T:G	R266S	0.998
20:44943481:C:G	R266T	0.998
20:44943579:T:A	R233S	0.998
20:44943579:T:G	R233S	0.998
20:44943570:G:C	C236W	0.997
20:44948829:C:T	G200D	0.997
20:44943179:A:G	L287P	0.996
20:44943475:G:T	A268D	0.996
20:44943528:G:C	C250W	0.996
20:44943530:A:G	C250R	0.996
20:44943572:A:G	C236R	0.996
20:44943578:C:G	A234P	0.996
20:44948781:T:G	Y216S	0.996
20:44948782:A:G	Y216H	0.996
20:44948830:C:G	G200R	0.996
20:44955176:C:G	R91P	0.996
20:44943521:C:G	A253P	0.995
20:44943571:C:T	C236Y	0.995
20:44948771:G:C	S219R	0.995
20:44948771:G:T	S219R	0.995
20:44948773:T:G	S219R	0.995
20:44943199:G:C	S280R	0.994
20:44943199:G:T	S280R	0.994
20:44943201:T:G	S280R	0.994
20:44943517:A:G	L254P	0.994
20:44943542:C:G	A246P	0.994

dbSNP variants (sampled 300 via entrez): RS1000049628 (20:44959393 A>G), RS1000275661 (20:44952941 G>A,C), RS1000305481 (20:44958326 T>A,C,G), RS1000397922 (20:44945496 G>A), RS1000409621 (20:44945226 A>G), RS1000626382 (20:44953221 G>T), RS1000731425 (20:44947071 T>C), RS1001015223 (20:44954526 G>A,T), RS1001066216 (20:44951966 C>T), RS1001166236 (20:44959958 G>A,C), RS1001197249 (20:44952804 A>G), RS1001298405 (20:44959214 A>G), RS1001317276 (20:44944461 A>G), RS1001906003 (20:44947229 T>C,G), RS1002396670 (20:44945337 C>A)

Disease associations

OMIM: gene MIM:616049 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST008103_18	Bipolar disorder	1.000000e-08

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6066215 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.66	Kd	21.89	nM	CHEMBL5653589
7.28	ED50	52.53	nM	CHEMBL5653589
5.52	IC50	3000	nM	CHEMBL5558181
5.40	IC50	4000	nM	CHEMBL5558644

PubChem BioAssay actives

3 with measured affinity, of 5 total; 3 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149632: Binding affinity to human TOMM34 incubated for 45 mins by Kinobead based pull down assay	kd	0.0219	uM
N,N’-bis(3-carbamoyl-4,5-dimethylthiophen-2-yl)pentanediamide	2084034: Inhibition of GST-tagged human TOM34 expressed in Escherichia coli BL21 (DE3) using NH2-EDASRMEEVD-COOH peptide as substrate preincubated for 15 mins followed by substrate addition measured after 15 mins by Alpha Screen assay	ic50	3.0000	uM
methyl 2-[[2-[[5,6-bis(furan-2-yl)-1,2,4-triazin-3-yl]sulfanyl]acetyl]amino]-4,5-dimethylthiophene-3-carboxylate	2084034: Inhibition of GST-tagged human TOM34 expressed in Escherichia coli BL21 (DE3) using NH2-EDASRMEEVD-COOH peptide as substrate preincubated for 15 mins followed by substrate addition measured after 15 mins by Alpha Screen assay	ic50	4.0000	uM

CTD chemical–gene interactions

47 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Acetaminophen	increases expression	2
Air Pollutants	increases expression, decreases expression, increases abundance	2
Particulate Matter	increases expression, decreases expression, increases abundance	2
aristolochic acid I	increases expression	1
FR900359	decreases phosphorylation	1
bisphenol A	decreases expression	1
sodium arsenate	increases abundance, increases expression	1
titanium dioxide	decreases expression	1
methylparaben	increases expression	1
mono-(2-ethylhexyl)phthalate	decreases expression	1
sodium arsenite	increases expression	1
perfluorooctanoic acid	increases expression	1
manganese chloride	increases abundance, increases expression	1
ochratoxin A	increases expression	1
ferrous chloride	increases expression	1
nickel sulfate	increases expression	1
4-aminophenylarsenoxide	affects binding, decreases reaction	1
di-n-butylphosphoric acid	affects expression	1
CGP 52608	affects binding, increases reaction	1
deguelin	decreases expression	1
pyrimidifen	decreases expression	1
bisphenol B	increases expression	1
abrine	increases expression	1
Arsenic Trioxide	affects binding, decreases reaction	1
Arsenic	increases abundance, increases expression	1
Benzo(a)pyrene	decreases methylation	1
Benztropine	decreases expression, affects cotreatment, increases expression	1
Cannabidiol	decreases expression	1
Cuprizone	affects cotreatment, increases expression	1
Dichlorodiphenyl Dichloroethylene	increases expression	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5524507	Binding	Inhibition of GST-tagged human TOM34 expressed in Escherichia coli BL21 (DE3) using NH2-EDASRMEEVD-COOH peptide as substrate preincubated for 15 mins followed by substrate addition measured after 15 mins by Alpha Screen assay	Structure-based discovery of small molecule inhibitors of FKBP51-Hsp90 protein-protein interaction. — Eur J Med Chem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2JA	Abcam HeLa TOMM34 KO	Cancer cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.