TRAM1

Summary

TRAM1 (translocation associated membrane protein 1, HGNC:20568) is a protein-coding gene on chromosome 8q13.3, encoding Translocating chain-associated membrane protein 1 (Q15629). Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel.

This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause.

Source: NCBI Gene 23471 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 56 total
• Druggable target: yes
• MANE Select transcript: NM_014294

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:20568
Approved symbol	TRAM1
Name	translocation associated membrane protein 1
Location	8q13.3
Locus type	gene with protein product
Status	Approved
Aliases	TRAM, TRAMP
Ensembl gene	ENSG00000067167
Ensembl biotype	protein_coding
OMIM	605190
Entrez	23471

Gene structure

Transcript identifiers

Ensembl transcripts: 17 — 15 protein_coding, 2 protein_coding_CDS_not_defined

ENST00000262213, ENST00000518678, ENST00000520700, ENST00000521049, ENST00000521425, ENST00000884663, ENST00000884664, ENST00000884665, ENST00000884666, ENST00000884667, ENST00000884668, ENST00000884669, ENST00000884670, ENST00000952367, ENST00000952368, ENST00000952369, ENST00000952370

RefSeq mRNA: 3 — MANE Select: NM_014294 NM_001317804, NM_001317805, NM_014294

CCDS: CCDS6207, CCDS83300

Canonical transcript exons

ENST00000262213 — 11 exons

Exon	Start	End
ENSE00000819638	70608077	70608416
ENSE00000980832	70594506	70594590
ENSE00000980835	70583650	70583793
ENSE00000980836	70583164	70583324
ENSE00003509824	70586895	70586999
ENSE00003510441	70597895	70598011
ENSE00003518934	70587106	70587176
ENSE00003563640	70600019	70600082
ENSE00003587566	70598134	70598255
ENSE00003690798	70596263	70596321
ENSE00003847185	70573218	70575005

Expression profiles

Bgee: expression breadth ubiquitous, 303 present calls, max score 99.81.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 130.4266 / max 952.0787, expressed in 1823 samples.

FANTOM5 promoters (9 alternative TSS)

Promoter ID	TPM avg	Samples expressed
93524	100.4300	1823
93525	12.6208	1799
93522	7.4189	1734
93523	7.1654	1713
93520	1.4149	826
93519	0.4817	260
93521	0.3878	177
93526	0.3426	148
205219	0.1646	77

Top tissues by expression

303 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
choroid plexus epithelium	UBERON:0003911	99.81	gold quality
visceral pleura	UBERON:0002401	99.64	gold quality
germinal epithelium of ovary	UBERON:0001304	99.54	gold quality
caput epididymis	UBERON:0004358	99.52	gold quality
corpus epididymis	UBERON:0004359	99.49	gold quality
parotid gland	UBERON:0001831	99.45	gold quality
pleura	UBERON:0000977	99.43	gold quality
parietal pleura	UBERON:0002400	99.37	gold quality
epididymis	UBERON:0001301	99.20	gold quality
tibia	UBERON:0000979	99.10	gold quality
seminal vesicle	UBERON:0000998	99.04	gold quality
lower lobe of lung	UBERON:0008949	98.93	gold quality
epithelium of nasopharynx	UBERON:0001951	98.91	gold quality
nasopharynx	UBERON:0001728	98.89	gold quality
periodontal ligament	UBERON:0008266	98.89	gold quality
superficial temporal artery	UBERON:0001614	98.87	gold quality
palpebral conjunctiva	UBERON:0001812	98.86	gold quality
eye	UBERON:0000970	98.73	gold quality
jejunal mucosa	UBERON:0000399	98.70	gold quality
pericardium	UBERON:0002407	98.70	gold quality
islet of Langerhans	UBERON:0000006	98.69	gold quality
cartilage tissue	UBERON:0002418	98.68	gold quality
stromal cell of endometrium	CL:0002255	98.65	gold quality
endometrium	UBERON:0001295	98.64	gold quality
body of pancreas	UBERON:0001150	98.62	gold quality
cauda epididymis	UBERON:0004360	98.62	gold quality
pancreas	UBERON:0001264	98.59	gold quality
upper leg skin	UBERON:0004262	98.59	gold quality
cardia of stomach	UBERON:0001162	98.51	gold quality
synovial joint	UBERON:0002217	98.48	gold quality

Single-cell (SCXA)

Detected in 13 experiment(s), a significant marker in 11.

Experiment	Marker?	Max mean expression
E-CURD-88	yes	82.87
E-HCAD-1	yes	50.64
E-MTAB-9467	yes	45.68
E-CURD-46	yes	44.19
E-CURD-122	yes	41.69
E-HCAD-4	yes	41.13
E-MTAB-8410	yes	31.45
E-HCAD-13	yes	11.79
E-MTAB-9801	yes	6.44
E-GEOD-130148	yes	5.17
E-MTAB-6524	no	147.60
E-HCAD-5	no	2.25
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

180 targeting TRAM1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-9-5P	100.00	72.28	2361
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-6833-3P	100.00	70.63	3197
HSA-MIR-4768-5P	100.00	69.49	2861
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-513B-5P	99.99	69.96	2150
HSA-MIR-4282	99.99	75.36	6408
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-499A-5P	99.98	70.79	1323
HSA-MIR-2110	99.96	66.68	1930
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-551B-5P	99.96	71.28	3493
HSA-MIR-548AT-5P	99.96	70.83	2666
HSA-MIR-3658	99.96	73.87	4379
HSA-MIR-548AB	99.95	71.31	3488
HSA-MIR-559	99.95	72.28	3609

Literature-anchored findings (GeneRIF, showing 4)

• TRAM1 protect HepG2 cells from palmitate-induced insulin resistance. The protection of TRAM1 to HepG2 cells is through endoplasmic reticulum stress-JNK pathway. (PMID:25600807)
• The human TRAM1 locus expresses circular RNAs. (PMID:34764360)
• Identification of TRAMs as sphingolipid-binding proteins using a photoactivatable and clickable short-chain ceramide analog. (PMID:34793833)
• The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication. (PMID:37768083)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	tram1	ENSDARG00000019137
mus_musculus	Tram1	ENSMUSG00000025935
rattus_norvegicus	Tram1	ENSRNOG00000007892
drosophila_melanogaster	TRAM	FBGN0040340
caenorhabditis_elegans		WBGENE00007696

Paralogs (2): TRAM2 (ENSG00000065308), TRAM1L1 (ENSG00000174599)

Protein

Protein identifiers

Translocating chain-associated membrane protein 1 — Q15629 (reviewed: Q15629)

All UniProt accessions (4): Q15629, E5RFP8, G3XAN4, Q6FHL3

UniProt curated annotations — full annotation on UniProt →

Function. Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel. Regulates the exposure of nascent secretory protein chain to the cytosol during translocation into the ER. May affect the phospholipid bilayer in the vicinity of the lateral gate of the SEC61 channel, thereby facilitating ER protein transport. Intimately associates with transmembrane (TM) domain of nascent membrane proteins during the entire integration process into the ER membrane. Associates with the second TM domain of G-protein-coupled receptor opsin/OPSD nascent chain in the ER membrane, which may facilitate its integration into the membrane. Under conditions of ER stress, participates in the disposal of misfolded ER membrane proteins during the unfolded protein response (UPR), an integrated stress response (ISR) pathway, by selectively retrotranslocating misfolded ER-membrane proteins from the ER into the cytosol where they are ubiquitinated and degraded by the proteasome. (Microbial infection) In case of cytomegalovirus infection, participates in US2- and US11-mediated ER-to-cytosol retrotranslocation and subsequent degradation of major histocompatibility complex (MHC) class I heavy chains, thereby decreasing the immune detection by cytotoxic T-cells.

Subunit / interactions. Interacts with SEC61B. May interact with Derlin-1/DERL1. (Microbial infection) Interacts with human cytomegalovirus/HHV-5 proteins US2 and US11.

Subcellular location. Endoplasmic reticulum membrane.

Post-translational modifications. N-glycosylated.

Induction. Up-regulated upon endoplasmic reticulum stress.

Similarity. Belongs to the TRAM family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q15629-1	1	yes
Q15629-2	2

RefSeq proteins (3): NP_001304733, NP_001304734, NP_055109* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR006634	TLC-dom	Domain
IPR016447	Translocation_assoc_membrane	Family

Pfam: PF03798

UniProt features (25 total): topological domain 9, transmembrane region 8, compositionally biased region 2, chain 1, domain 1, region of interest 1, modified residue 1, glycosylation site 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q15629-F1	84.56	0.64

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 365

Glycosylation sites (1): 56

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-1799339	SRP-dependent cotranslational protein targeting to membrane

MSigDB gene sets: 284 (showing top): GSE18804_BRAIN_VS_COLON_TUMORAL_MACROPHAGE_DN, GSE18804_SPLEEN_MACROPHAGE_VS_TUMORAL_MACROPHAGE_DN, GGGACCA_MIR133A_MIR133B, TGCGCANK_UNKNOWN, GOBP_INTRACELLULAR_PROTEIN_TRANSPORT, TGCTGCT_MIR15A_MIR16_MIR15B_MIR195_MIR424_MIR497, GCM_MSN, TTTGTAG_MIR520D, GOBP_PROTEIN_TARGETING, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ENDOPLASMIC_RETICULUM, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ORGANELLE, CACCAGC_MIR138, CAGCTG_AP4_Q5, BRUECKNER_TARGETS_OF_MIRLET7A3_DN, SHAFFER_IRF4_TARGETS_IN_PLASMA_CELL_VS_MATURE_B_LYMPHOCYTE

GO Biological Process (5): cotranslational protein targeting to membrane (GO:0006613), SRP-dependent cotranslational protein targeting to membrane, translocation (GO:0006616), response to unfolded protein (GO:0006986), protein insertion into ER membrane (GO:0045048), protein transport (GO:0015031)

GO Molecular Function (2): signaling receptor activity (GO:0038023), protein binding (GO:0005515)

GO Cellular Component (3): endoplasmic reticulum (GO:0005783), endoplasmic reticulum membrane (GO:0005789), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Translation	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein targeting to membrane	1
SRP-dependent cotranslational protein targeting to membrane	1
intracellular protein transmembrane transport	1
response to topologically incorrect protein	1
endoplasmic reticulum organization	1
protein localization to organelle	1
protein insertion into membrane	1
transport	1
intracellular protein localization	1
establishment of protein localization	1
molecular transducer activity	1
binding	1
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
cellular anatomical structure	1

Protein interactions and networks

STRING

644 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TRAM1	SSR2	P43308	889
TRAM1	APLP2	Q06481	466
TRAM1	CCDC47	Q96A33	448
TRAM1	SEC63	Q9UGP8	438
TRAM1	SEC61A1	P38378	430
TRAM1	VKORC1	Q9BQB6	416
TRAM1	TMCO1	Q9UM00	412
TRAM1	CLN8	Q9UBY8	407
TRAM1	TM4SF20	Q53R12	405
TRAM1	EMILIN2	Q9BXX0	382
TRAM1	CWF19L2	Q2TBE0	363
TRAM1	HID1	Q8IV36	363
TRAM1	SMG5	Q9UPR3	354
TRAM1	TMEM231	Q9H6L2	351
TRAM1	TLCD1	Q96CP7	349

IntAct

72 interactions, top by confidence:

A	B	Type	Score
	DDX3X	psi-mi:“MI:0914”(association)	0.630
TRAM1	BCAP31	psi-mi:“MI:0915”(physical association)	0.600
BCAP31	TRAM1	psi-mi:“MI:0915”(physical association)	0.600
BCAP31	TRAM1	psi-mi:“MI:0403”(colocalization)	0.600
GUCA2A	TRAM1	psi-mi:“MI:0915”(physical association)	0.560
SLC6A8	ILVBL	psi-mi:“MI:0914”(association)	0.530
UNC93B1	GPR89A	psi-mi:“MI:0914”(association)	0.530
TRAM1	GOPC	psi-mi:“MI:0915”(physical association)	0.400
TRAM1	DRD2	psi-mi:“MI:0915”(physical association)	0.370
TRAM1	CREB3	psi-mi:“MI:0915”(physical association)	0.370
TRAM1	GAMMAHV.ORF39	psi-mi:“MI:0915”(physical association)	0.370
TK	AP3B1	psi-mi:“MI:0914”(association)	0.350
E6	TRAFD1	psi-mi:“MI:0914”(association)	0.350
	ESYT2	psi-mi:“MI:0914”(association)	0.350
E5	ESYT2	psi-mi:“MI:0914”(association)	0.350
	PGRMC1	psi-mi:“MI:0914”(association)	0.350
	SNAP23	psi-mi:“MI:0914”(association)	0.350
	VWA8	psi-mi:“MI:0914”(association)	0.350
	HAX1	psi-mi:“MI:0914”(association)	0.350
BVLF1	VWA8	psi-mi:“MI:0914”(association)	0.350
BFRF1A	SHTN1	psi-mi:“MI:0914”(association)	0.350
	TNFRSF10B	psi-mi:“MI:0914”(association)	0.350
Prdm16	ESYT2	psi-mi:“MI:0914”(association)	0.350
Mecom	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (124): TRAM1 (Proximity Label-MS), TRAM1 (Two-hybrid), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Reconstituted Complex), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS), TRAM1 (Affinity Capture-MS)

ESM2 similar proteins: A2AKM2, A4FUY9, A5D6V4, A7YY55, A8WGS4, Q01685, Q0P5C7, Q15629, Q3T124, Q4R8A8, Q4V8U5, Q5BJF2, Q5GH60, Q5GH61, Q5GH68, Q5HZE5, Q5ND56, Q5NVQ2, Q5R7Z3, Q5U2T1, Q5VWC8, Q5XI41, Q5ZM57, Q60457, Q6DED0, Q6GLX2, Q6GNB5, Q6P4N1, Q6PP77, Q6YWS8, Q7SY06, Q84QC0, Q86X19, Q8CGF5, Q8K0U3, Q8K2C9, Q8N609, Q8QZR0, Q8R000, Q8VZB2

Diamond homologs: Q01685, Q15035, Q15629, Q5E9R6, Q5R7Z3, Q5XI41, Q6DED0, Q8N609, Q8QZR0, Q91V04, Q924Z5, Q9GKZ4, A6ZSP9, O59735, P27544, P38703, P78970, Q1A3B0, Q3ZBF8, Q6EUN0, Q6NQI8, Q6YWS8, Q6ZMG9, Q84QC0, Q8C172, Q8IU89, Q8N5B7, Q8W4Y5, Q924Z4, Q96G23, Q9D6J1, Q9D6K9, Q9HA82, Q9LDF2, Q9LJK3, Q9M6A3

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 76 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Amino acid transport across the plasma membrane	5	39.5×	1e-05
R-HSA-425366	6	28.6×	1e-05
SLC-mediated transmembrane transport	8	12.5×	1e-05
Transport of small molecules	8	5.3×	3e-03

GO biological processes:

GO term	Partners	Fold	FDR
amino acid transport	8	46.2×	3e-09
sodium ion transmembrane transport	5	18.8×	2e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

56 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	29
Likely benign	4
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

1512 predictions. Top by Δscore:

Variant	Effect	Δscore
8:70575006:C:CC	acceptor_gain	1.0000
8:70583151:A:AC	donor_gain	1.0000
8:70583152:C:CC	donor_gain	1.0000
8:70583320:CGATT:C	acceptor_gain	1.0000
8:70583323:TT:T	acceptor_gain	1.0000
8:70583325:C:CC	acceptor_gain	1.0000
8:70587104:A:AC	donor_gain	1.0000
8:70587105:C:CC	donor_gain	1.0000
8:70587105:CTT:C	donor_gain	1.0000
8:70587173:CTTC:C	acceptor_gain	1.0000
8:70587175:TCCT:T	acceptor_loss	1.0000
8:70587177:C:CA	acceptor_loss	1.0000
8:70587177:C:CC	acceptor_gain	1.0000
8:70587178:T:A	acceptor_loss	1.0000
8:70594504:A:AC	donor_gain	1.0000
8:70594505:C:CT	donor_gain	1.0000
8:70595199:T:A	donor_gain	1.0000
8:70596261:A:AC	donor_gain	1.0000
8:70596262:C:CC	donor_gain	1.0000
8:70596262:CGT:C	donor_gain	1.0000
8:70596318:TTTCC:T	acceptor_loss	1.0000
8:70596320:TCCTA:T	acceptor_loss	1.0000
8:70596321:CCTA:C	acceptor_loss	1.0000
8:70596322:CTAAG:C	acceptor_loss	1.0000
8:70597889:A:AC	donor_gain	1.0000
8:70597889:AC:A	donor_loss	1.0000
8:70597890:C:CC	donor_gain	1.0000
8:70597890:CT:C	donor_loss	1.0000
8:70597891:TT:T	donor_loss	1.0000
8:70597892:TAC:T	donor_loss	1.0000

AlphaMissense

2462 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
8:70608146:G:C	F18L	0.997
8:70608146:G:T	F18L	0.997
8:70608148:A:G	F18L	0.997
8:70583769:A:C	F257L	0.996
8:70583769:A:T	F257L	0.996
8:70583771:A:G	F257L	0.996
8:70586931:C:G	R237P	0.996
8:70597915:C:G	G136R	0.996
8:70597953:C:T	G123D	0.996
8:70597957:A:G	S122P	0.996
8:70583270:G:C	F315L	0.995
8:70583270:G:T	F315L	0.995
8:70583272:A:G	F315L	0.995
8:70583790:A:C	F250L	0.995
8:70583790:A:T	F250L	0.995
8:70583792:A:G	F250L	0.995
8:70597943:A:C	S126R	0.995
8:70597943:A:T	S126R	0.995
8:70597945:T:G	S126R	0.995
8:70598160:C:G	A95P	0.995
8:70598207:T:G	D79A	0.995
8:70598208:C:G	D79H	0.995
8:70583302:A:G	C305R	0.994
8:70583783:A:G	W253R	0.994
8:70583783:A:T	W253R	0.994
8:70586986:C:G	G219R	0.994
8:70586986:C:T	G219R	0.994
8:70594550:A:G	W176R	0.994
8:70594550:A:T	W176R	0.994
8:70594578:C:A	K166N	0.993

dbSNP variants (sampled 300 via entrez): RS1000038123 (8:70575410 TTTTA>T), RS1000046525 (8:70578067 G>A,T), RS1000153662 (8:70606288 G>A,T), RS1000166349 (8:70597983 T>C), RS1000224668 (8:70604612 G>C), RS1000265877 (8:70581335 T>G), RS1000296846 (8:70604415 T>C), RS1000531784 (8:70594108 C>T), RS1000562253 (8:70606302 C>T), RS1000676487 (8:70605914 T>A,C), RS1000755438 (8:70607628 C>A,T), RS1000865207 (8:70584563 C>A,T), RS1000880117 (8:70594646 A>C), RS1000965631 (8:70576958 G>C), RS1000996619 (8:70592233 T>C)

Disease associations

OMIM: gene MIM:605190 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST001776_2	Cardiac Troponin-T levels	9.000000e-09
GCST003448_1	Erythrocyte cadmium concentration in never smokers	3.000000e-11
GCST006291_69	Spherical equivalent or myopia (age of diagnosis)	4.000000e-10
GCST010002_303	Refractive error	1.000000e-26

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0005043	cardiac troponin T measurement
EFO:0004847	age at onset

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067446 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.44	Kd	362.2	nM	CHEMBL5653589
6.44	ED50	362.2	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149653: Binding affinity to human TRAM1 incubated for 45 mins by Kinobead based pull down assay	kd	0.3622	uM

CTD chemical–gene interactions

48 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	affects binding, increases reaction, decreases expression, increases expression	5
Cyclosporine	decreases methylation, increases expression	3
Air Pollutants	affects cotreatment, increases abundance, increases oxidation, decreases expression	2
Benzo(a)pyrene	decreases methylation, increases mutagenesis	2
Cisplatin	affects cotreatment, decreases expression	2
Tunicamycin	increases expression	2
Thapsigargin	increases expression	2
Particulate Matter	decreases expression, increases abundance, affects cotreatment	2
aristolochic acid I	decreases expression	1
FR900359	decreases phosphorylation	1
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
alpha-pinene	affects cotreatment, increases oxidation, increases abundance	1
bisphenol A	affects expression	1
kojic acid	decreases expression	1
coumarin	decreases phosphorylation	1
cupric oxide	decreases expression	1
methacrylaldehyde	increases oxidation, increases abundance, affects cotreatment	1
beta-methylcholine	affects expression	1
bicalutamide	increases expression	1
CGP 52608	affects binding, increases reaction	1
chloropicrin	affects expression	1
pyrachlostrobin	increases expression	1
jinfukang	affects cotreatment, decreases expression	1
picoxystrobin	increases expression	1
Vorinostat	increases expression	1
Acrolein	affects cotreatment, increases oxidation, increases abundance	1
Ethanol	affects cotreatment, decreases expression, increases abundance	1
Bile Acids and Salts	increases expression	1
Doxorubicin	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652695	Binding	Binding affinity to human TRAM1 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.