TRIM4

Summary

TRIM4 (tripartite motif containing 4, HGNC:16275) is a protein-coding gene on chromosome 7q22.1, encoding E3 ubiquitin-protein ligase TRIM4 (Q9C037). E3 ubiquitin-protein ligase.

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.

Source: NCBI Gene 89122 — RefSeq curated summary.

At a glance

• GWAS associations: 7
• Clinical variants (ClinVar): 81 total
• Druggable target: yes
• MANE Select transcript: NM_033091

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:16275
Approved symbol	TRIM4
Name	tripartite motif containing 4
Location	7q22.1
Locus type	gene with protein product
Status	Approved
Aliases	RNF87
Ensembl gene	ENSG00000146833
Ensembl biotype	protein_coding
Entrez	89122

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 10 protein_coding, 1 retained_intron

ENST00000349062, ENST00000354241, ENST00000355947, ENST00000447480, ENST00000496896, ENST00000904368, ENST00000904369, ENST00000953518, ENST00000953519, ENST00000953520, ENST00000953521

RefSeq mRNA: 2 — MANE Select: NM_033091 NM_033017, NM_033091

CCDS: CCDS5678, CCDS5679

Canonical transcript exons

ENST00000349062 — 6 exons

Exon	Start	End
ENSE00000977649	99903218	99903315
ENSE00001168333	99903576	99903598
ENSE00001168341	99908582	99908812
ENSE00001925951	99919009	99919530
ENSE00002492388	99909565	99909660
ENSE00003908286	99890403	99892746

Expression profiles

Bgee: expression breadth ubiquitous, 254 present calls, max score 99.87.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 22.5758 / max 177.8033, expressed in 1757 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
85123	13.3215	1735
85122	5.3760	1658
85125	1.6394	969
85124	1.3493	889
85121	0.8894	627

Top tissues by expression

255 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
buccal mucosa cell	CL:0002336	99.87	gold quality
tendon of biceps brachii	UBERON:0008188	97.64	gold quality
upper leg skin	UBERON:0004262	96.27	gold quality
medial globus pallidus	UBERON:0002477	96.02	gold quality
ileal mucosa	UBERON:0000331	95.19	gold quality
globus pallidus	UBERON:0001875	95.13	gold quality
calcaneal tendon	UBERON:0003701	95.07	gold quality
tendon	UBERON:0000043	95.05	gold quality
sural nerve	UBERON:0015488	94.76	gold quality
tibialis anterior	UBERON:0001385	94.52	gold quality
cardia of stomach	UBERON:0001162	94.28	gold quality
subthalamic nucleus	UBERON:0001906	94.14	gold quality
ventral tegmental area	UBERON:0002691	94.06	gold quality
mammary duct	UBERON:0001765	94.05	gold quality
superior surface of tongue	UBERON:0007371	94.05	gold quality
epithelium of mammary gland	UBERON:0003244	94.04	gold quality
superior vestibular nucleus	UBERON:0007227	94.04	gold quality
trigeminal ganglion	UBERON:0001675	94.00	gold quality
trachea	UBERON:0003126	93.96	gold quality
saphenous vein	UBERON:0007318	93.89	gold quality
oviduct epithelium	UBERON:0004804	93.81	gold quality
medulla oblongata	UBERON:0001896	93.78	gold quality
pharyngeal mucosa	UBERON:0000355	93.73	gold quality
pylorus	UBERON:0001166	93.64	gold quality
sperm	CL:0000019	93.61	gold quality
epithelial cell of pancreas	CL:0000083	93.34	gold quality
inferior vagus X ganglion	UBERON:0005363	93.18	gold quality
germinal epithelium of ovary	UBERON:0001304	93.16	gold quality
dorsal plus ventral thalamus	UBERON:0001897	93.01	gold quality
lower lobe of lung	UBERON:0008949	92.72	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

88 targeting TRIM4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-188-3P	100.00	68.76	1240
HSA-MIR-1252-5P	100.00	69.80	2774
HSA-MIR-4747-5P	100.00	67.90	2681
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-5196-5P	100.00	67.98	2761
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-548P	99.98	72.25	3784
HSA-LET-7C-3P	99.95	73.42	2862
HSA-MIR-454-3P	99.91	74.01	1925
HSA-MIR-130A-3P	99.90	73.31	1861
HSA-MIR-130B-3P	99.90	73.27	1850
HSA-MIR-301A-3P	99.90	73.15	1839
HSA-MIR-301B-3P	99.90	73.19	1836
HSA-MIR-3666	99.90	73.24	1833
HSA-MIR-4295	99.90	73.11	1838
HSA-MIR-4753-3P	99.90	71.03	3786
HSA-MIR-4447	99.85	67.81	2900
HSA-MIR-548AJ-5P	99.78	71.12	3085
HSA-MIR-548F-5P	99.78	71.02	3093
HSA-MIR-548G-5P	99.78	71.12	3085
HSA-MIR-548X-5P	99.78	71.12	3085
HSA-MIR-187-5P	99.74	70.26	1404
HSA-MIR-4802-3P	99.72	70.13	1273
HSA-MIR-6762-3P	99.66	66.94	1188
HSA-MIR-4666B	99.64	68.69	1282
HSA-MIR-7156-5P	99.64	68.81	1369

Literature-anchored findings (GeneRIF, showing 8)

• ZNF198-FGFR1 is associated with phosphorylation of several proteins including SSBP2, ABL, FLJ14235, CALM and TRIM4 proteins. (PMID:19658100)
• TRIM4 is an important regulator of the virus-induced interferon induction pathways by mediating RIG-I for K63-linked ubiquitination. (PMID:24755855)
• Findings suggest that spinal cord tissues in cases with neural tube defects (NTDs) had a different genome-wide methylation pattern compared to controls. Abnormal methylation patterns in TRIM4 in immunity pathways might be involved in NTD pathogenesis. Genetic variants in TRIM4 genes only slightly contribute to the etiology of human NTDs. (PMID:30709423)
• TRIM4 interacts with TRPM8 and regulates its channel function through K423-mediated ubiquitination. (PMID:33037615)
• The E3 Ligase TRIM4 Facilitates SET Ubiquitin-Mediated Degradation to Enhance ER-alpha Action in Breast Cancer. (PMID:35843886)
• TRIM4 Expression Related to Malignant Progression and Cisplatin Resistance in Osteosarcoma. (PMID:37115387)
• SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination. (PMID:37956198)
• The TRIM4 E3 ubiquitin ligase degrades TPL2 and is modulated by oncogenic KRAS. (PMID:39178114)

Cross-species orthologs

0 orthologs

Paralogs (80): MID2 (ENSG00000080561), TRIM9 (ENSG00000100505), MID1 (ENSG00000101871), MEFV (ENSG00000103313), TRIM35 (ENSG00000104228), TRIM14 (ENSG00000106785), TRIM37 (ENSG00000108395), TRIM2 (ENSG00000109654), TRIM3 (ENSG00000110171), TRIM38 (ENSG00000112343), TRIM62 (ENSG00000116525), TRIM67 (ENSG00000119283), TRIM32 (ENSG00000119401), BSPRY (ENSG00000119411), TRIM25 (ENSG00000121060), TRIM6 (ENSG00000121236), TRIM24 (ENSG00000122779), TRIM51 (ENSG00000124900), TRIM28 (ENSG00000130726), TRIM21 (ENSG00000132109), TRIM5 (ENSG00000132256), TRIM22 (ENSG00000132274), TRIM47 (ENSG00000132481), TRIM45 (ENSG00000134253), TRIM29 (ENSG00000137699), TRIM54 (ENSG00000138100), PML (ENSG00000140464), TRIM65 (ENSG00000141569), TRIM43B (ENSG00000144010), TRIM43 (ENSG00000144015), TRIM7 (ENSG00000146054), TRIM41 (ENSG00000146063), TRIM50 (ENSG00000146755), TRIM55 (ENSG00000147573), TRIM48 (ENSG00000150244), TRIM36 (ENSG00000152503), TRIM11 (ENSG00000154370), TRIM74 (ENSG00000155428), TRIM42 (ENSG00000155890), TRIM63 (ENSG00000158022)

Protein

Protein identifiers

E3 ubiquitin-protein ligase TRIM4 — Q9C037 (reviewed: Q9C037)

Alternative names: RING finger protein 87, RING-type E3 ubiquitin transferase TRIM4, Tripartite motif-containing protein 4

All UniProt accessions (2): Q9C037, H7C0Q6

UniProt curated annotations — full annotation on UniProt →

Function. E3 ubiquitin-protein ligase. Mediates ‘Lys-63’-linked polyubiquitination of the innate immune receptor RIGI, this linkage doesn’t lead to proteasomal degradation but seems to enhance IFN induction.

Subunit / interactions. Homotrimer.

Subcellular location. Cytoplasm.

Pathway. Protein modification; protein ubiquitination.

Similarity. Belongs to the TRIM/RBCC family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q9C037-1	Alpha	yes
Q9C037-2	Beta
Q9C037-3	Gamma

RefSeq proteins (2): NP_148977, NP_149082* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000315	Znf_B-box	Domain
IPR001841	Znf_RING	Domain
IPR001870	B30.2/SPRY	Domain
IPR003877	SPRY_dom	Domain
IPR003879	Butyrophylin_SPRY	Domain
IPR013083	Znf_RING/FYVE/PHD	Homologous_superfamily
IPR013320	ConA-like_dom_sf	Homologous_superfamily
IPR017907	Znf_RING_CS	Conserved_site
IPR035829	PRY/SPRY_TRIM4	Domain
IPR043136	B30.2/SPRY_sf	Homologous_superfamily
IPR050143	TRIM/RBCC	Family

Pfam: PF00622, PF00643, PF15227

UniProt features (16 total): binding site 4, splice variant 3, sequence variant 2, zinc finger region 2, chain 1, domain 1, mutagenesis site 1, sequence conflict 1, coiled-coil region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9C037-F1	83.42	0.42

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 87; 90; 109; 115

Mutagenesis-validated functional residues (1):

Position	Phenotype
27	abolishes ligase activity.

Function

Pathways and Gene Ontology

Reactome pathways

9 pathways

ID	Pathway
R-HSA-168928	DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5689896	Ovarian tumor domain proteases
R-HSA-918233	TRAF3-dependent IRF activation pathway
R-HSA-933541	TRAF6 mediated IRF7 activation
R-HSA-933542	TRAF6 mediated NF-kB activation
R-HSA-933543	NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10
R-HSA-936440	Negative regulators of DDX58/IFIH1 signaling
R-HSA-9705671	SARS-CoV-2 activates/modulates innate and adaptive immune responses
R-HSA-983168	Antigen processing: Ubiquitination & Proteasome degradation

MSigDB gene sets: 74 (showing top): REACTOME_DDX58_IFIH1_MEDIATED_INDUCTION_OF_INTERFERON_ALPHA_BETA, REACTOME_INNATE_IMMUNE_SYSTEM, REACTOME_ADAPTIVE_IMMUNE_SYSTEM, REACTOME_CLASS_I_MHC_MEDIATED_ANTIGEN_PROCESSING_PRESENTATION, REACTOME_ANTIGEN_PROCESSING_UBIQUITINATION_PROTEASOME_DEGRADATION, MODULE_503, GOBP_DEFENSE_RESPONSE_TO_OTHER_ORGANISM, MODULE_195, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, MODULE_147, MODULE_356, NUYTTEN_EZH2_TARGETS_DN, GARY_CD5_TARGETS_UP, KRIGE_RESPONSE_TO_TOSEDOSTAT_24HR_UP, GOMF_ACYLTRANSFERASE_ACTIVITY

GO Biological Process (4): regulation of gene expression (GO:0010468), protein ubiquitination (GO:0016567), innate immune response (GO:0045087), immune system process (GO:0002376)

GO Molecular Function (7): ubiquitin-protein transferase activity (GO:0004842), zinc ion binding (GO:0008270), identical protein binding (GO:0042802), ubiquitin protein ligase activity (GO:0061630), protein binding (GO:0005515), transferase activity (GO:0016740), metal ion binding (GO:0046872)

GO Cellular Component (3): cytoplasm (GO:0005737), cytosol (GO:0005829), plasma membrane (GO:0005886)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
DDX58/IFIH1-mediated induction of interferon-alpha/beta	5
Innate Immune System	1
Deubiquitination	1
SARS-CoV-2-host interactions	1
Class I MHC mediated antigen processing & presentation	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
gene expression	1
regulation of macromolecule biosynthetic process	1
protein modification by small protein conjugation	1
immune response	1
defense response to symbiont	1
biological_process	1
ubiquitin-like protein transferase activity	1
transition metal ion binding	1
protein binding	1
ubiquitin-protein transferase activity	1
ubiquitin-like protein ligase activity	1
binding	1
catalytic activity	1
cation binding	1
intracellular anatomical structure	1
cytoplasm	1
membrane	1
cell periphery	1

Protein interactions and networks

STRING

824 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TRIM4	MEX3C	Q5U5Q3	754
TRIM4	RIGI	O95786	713
TRIM4	RNF125	Q96EQ8	591
TRIM4	TRAT1	Q6PIZ9	583
TRIM4	RNF122	Q9H9V4	582
TRIM4	TRIM66	O15016	517
TRIM4	TRIM23	P36406	515
TRIM4	ZCCHC3	Q9NUD5	506
TRIM4	USP3	Q9Y6I4	502
TRIM4	MAVS	Q7Z434	491
TRIM4	TRIM42	Q8IWZ5	457
TRIM4	ZNF248	Q8NDW4	449
TRIM4	TRIM56	Q9BRZ2	433
TRIM4	TRIM9	Q9C026	430
TRIM4	USP15	Q9Y4E8	421

IntAct

24 interactions, top by confidence:

A	B	Type	Score
RAB31	CHM	psi-mi:“MI:0914”(association)	0.640
LTBR	ZNF724	psi-mi:“MI:0914”(association)	0.530
RAB39B	CBL	psi-mi:“MI:0914”(association)	0.530
TRIM4	P4HB	psi-mi:“MI:0915”(physical association)	0.400
MDM2	TRIM4	psi-mi:“MI:0915”(physical association)	0.370
TRIM4	TRIM41	psi-mi:“MI:0915”(physical association)	0.370
Anapc2	ANAPC15	psi-mi:“MI:0914”(association)	0.350
NA	IPO5	psi-mi:“MI:0914”(association)	0.350
METTL14	HMGB1P1	psi-mi:“MI:0914”(association)	0.350
rep	EIF3F	psi-mi:“MI:0914”(association)	0.350
NCAPD3	NDUFS8	psi-mi:“MI:0914”(association)	0.350
ARRDC3	ESYT2	psi-mi:“MI:0914”(association)	0.350
DUSP16	MEIOC	psi-mi:“MI:0914”(association)	0.350
TRIM69	POTEF	psi-mi:“MI:0914”(association)	0.350
FPR2	GPR89A	psi-mi:“MI:0914”(association)	0.350
KNSTRN	TRIM24	psi-mi:“MI:0914”(association)	0.350
TRAK1	PEX14	psi-mi:“MI:0914”(association)	0.350
NUBP1	YBEY	psi-mi:“MI:0914”(association)	0.350
B4GALT2	LENG9	psi-mi:“MI:0914”(association)	0.350
FPR2	SCAMP3	psi-mi:“MI:0914”(association)	0.350
RAB31	SCO1	psi-mi:“MI:0914”(association)	0.350
MFSD14A	FAM171A2	psi-mi:“MI:0914”(association)	0.350
CFTR	UBA6	psi-mi:“MI:2364”(proximity)	0.270

BioGRID (108): TRIM4 (Affinity Capture-MS), P4HB (Affinity Capture-MS), TRIM4 (Affinity Capture-MS), TRIM4 (Affinity Capture-MS), PRDX1 (Affinity Capture-Western), TRIM4 (Affinity Capture-Western), TRIM4 (Proximity Label-MS), TRIM4 (Affinity Capture-MS), P4HB (Affinity Capture-MS), TRIM4 (Affinity Capture-MS), TRIM4 (Affinity Capture-MS), TRIM4 (Affinity Capture-MS), TRIM4 (Affinity Capture-MS), TRIM4 (Affinity Capture-MS), TRIM4 (Proximity Label-MS)

ESM2 similar proteins: A0JPQ4, E1BD59, O15197, P0C0K6, P62603, Q14142, Q1XH17, Q1XH18, Q3UWZ0, Q5BK82, Q5JZY3, Q5M929, Q5NCC3, Q5RBG2, Q5RKG6, Q5TM55, Q5W0U4, Q640S6, Q6P6S3, Q6PGR9, Q6PJ69, Q6ZMU5, Q7TPM3, Q7YR32, Q80VI1, Q80X56, Q80YW5, Q810I1, Q810I2, Q865W2, Q86UV6, Q86UV7, Q86XT4, Q8BFW4, Q8BVW3, Q8BYG9, Q8C006, Q8C0E3, Q8IUD6, Q8K243

Diamond homologs: A0JN74, A6NCK2, A6NDI0, A6NGJ6, A6NI03, A6NK02, A6NLI5, B1H278, C9J1S8, K7N6K2, O00635, O15553, O19085, O77666, P0CI25, P0CI26, P14373, P15533, P18892, P19474, Q02084, Q0PF16, Q12899, Q13410, Q1ACD5, Q1ACD6, Q1ACD7, Q1ACD8, Q1XHU0, Q2T9Z0, Q2YEM8, Q2YEM9, Q2YEN0, Q2YEN2, Q3UWZ0, Q3ZEE5, Q495X7, Q587N6, Q587N7, Q58DK8

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
Ub:E2	“up-regulates activity”	TRIM4	ubiquitination
TRIM4	“down-regulates quantity”	RTN2	ubiquitination

Disease & clinical

Clinical variants and AI predictions

ClinVar

81 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	71
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1094 predictions. Top by Δscore:

Variant	Effect	Δscore
7:99903311:CACTC:C	acceptor_gain	1.0000
7:99903313:CTC:C	acceptor_gain	1.0000
7:99909559:CATTA:C	donor_loss	1.0000
7:99909560:ATTAC:A	donor_loss	1.0000
7:99909561:TTACC:T	donor_loss	1.0000
7:99909564:C:G	donor_loss	1.0000
7:99909666:A:T	acceptor_gain	1.0000
7:99916703:T:TA	donor_gain	1.0000
7:99892747:C:CC	acceptor_gain	0.9900
7:99903212:GCTCA:G	donor_loss	0.9900
7:99903213:CTCA:C	donor_loss	0.9900
7:99903214:TCA:T	donor_loss	0.9900
7:99903215:CACC:C	donor_loss	0.9900
7:99903216:A:AT	donor_loss	0.9900
7:99903217:CCTTG:C	donor_loss	0.9900
7:99903314:TCC:T	acceptor_loss	0.9900
7:99903315:CCTAA:C	acceptor_loss	0.9900
7:99903316:C:CC	acceptor_gain	0.9900
7:99903317:T:G	acceptor_loss	0.9900
7:99908572:A:C	donor_gain	0.9900
7:99908578:TCACC:T	donor_loss	0.9900
7:99908579:CAC:C	donor_loss	0.9900
7:99908580:A:C	donor_loss	0.9900
7:99908581:C:G	donor_loss	0.9900
7:99909567:T:A	donor_gain	0.9900
7:99909658:CTC:C	acceptor_gain	0.9900
7:99909658:CTCCT:C	acceptor_loss	0.9900
7:99909660:CCT:C	acceptor_loss	0.9900
7:99909661:C:CC	acceptor_gain	0.9900
7:99909661:C:T	acceptor_loss	0.9900

AlphaMissense

3101 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:99919309:G:C	F31L	0.993
7:99919309:G:T	F31L	0.993
7:99919311:A:G	F31L	0.993
7:99892522:A:G	W382R	0.992
7:99892522:A:T	W382R	0.992
7:99919368:A:G	C12R	0.992
7:99892520:C:A	W382C	0.991
7:99892520:C:G	W382C	0.991
7:99919086:A:G	C106R	0.991
7:99919111:G:C	F97L	0.991
7:99919111:G:T	F97L	0.991
7:99919113:A:G	F97L	0.991
7:99892482:C:T	G395E	0.989
7:99892483:C:A	G395W	0.989
7:99892289:G:C	F459L	0.987
7:99892289:G:T	F459L	0.987
7:99892291:A:G	F459L	0.987
7:99908771:A:C	F203L	0.987
7:99908771:A:T	F203L	0.987
7:99908773:A:G	F203L	0.987
7:99919257:A:G	C49R	0.986
7:99919312:G:C	N30K	0.986
7:99919312:G:T	N30K	0.986
7:99919315:G:C	H29Q	0.986
7:99919315:G:T	H29Q	0.986
7:99892290:A:G	F459S	0.985
7:99919359:A:G	C15R	0.984
7:99892294:A:G	S458P	0.983
7:99919076:C:G	C109S	0.983
7:99919077:A:T	C109S	0.983

dbSNP variants (sampled 300 via entrez): RS1000050800 (7:99918899 A>G), RS1000142055 (7:99906120 C>T), RS1000252278 (7:99901178 G>A), RS1000372733 (7:99918002 T>A,C,G), RS1000429951 (7:99918567 A>G), RS1000465681 (7:99895635 G>T), RS1000492294 (7:99913758 T>C), RS1000744202 (7:99907715 A>C,G), RS1000836869 (7:99898505 T>G), RS1000931580 (7:99890913 G>T), RS1001008891 (7:99892946 G>A), RS1001170262 (7:99897143 G>A), RS1001248050 (7:99902398 C>A,G), RS1001445377 (7:99896912 G>C), RS1001909349 (7:99892015 A>G)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

7 associations (top):

Study	Trait	p-value
GCST001038_4	Dehydroepiandrosterone sulphate levels	2.000000e-11
GCST005144_1	Tacrolimus trough concentration in kidney transplant patients	2.000000e-17
GCST005827_3	Estrone levels	6.000000e-14
GCST006249_23	Serum metabolite levels	2.000000e-21
GCST006249_85	Serum metabolite levels	3.000000e-15
GCST010002_259	Refractive error	3.000000e-16
GCST010083_144	Hemoglobin levels	1.000000e-13

EFO canonical traits (4, from GWAS)

EFO ID	Trait name
EFO:0004730	hormone measurement
EFO:0008458	tacrolimus measurement
EFO:0007970	estrone measurement
EFO:0004509	hemoglobin measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067377 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.85	Kd	141.4	nM	CHEMBL5653589
6.77	ED50	168.5	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149657: Binding affinity to human TRIM4 incubated for 45 mins by Kinobead based pull down assay	kd	0.1414	uM

CTD chemical–gene interactions

35 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	decreases expression, increases methylation	2
Cisplatin	affects expression, increases expression	2
Hydrogen Peroxide	affects cotreatment, increases expression, affects expression	2
aristolochic acid I	increases expression	1
afuresertib	increases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	affects cotreatment, increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
beta-methylcholine	affects expression	1
di-n-butylphosphoric acid	affects expression	1
CGP 52608	affects binding, increases reaction	1
ICG 001	increases expression	1
bisphenol S	affects cotreatment, increases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	increases expression	1
Irinotecan	increases expression	1
Temozolomide	increases expression	1
Decitabine	affects expression	1
Fulvestrant	increases methylation	1
Air Pollutants	affects expression, increases abundance	1
Dexamethasone	affects cotreatment, increases expression	1
Succimer	affects cotreatment, increases expression	1
Ethyl Methanesulfonate	increases expression	1
Formaldehyde	increases expression	1
Indomethacin	affects cotreatment, increases expression	1
Ivermectin	decreases expression	1
Methyl Methanesulfonate	increases expression	1
Ozone	affects expression, increases abundance	1
Quercetin	increases expression	1
Theophylline	increases expression, affects cotreatment	1
1-Methyl-3-isobutylxanthine	affects cotreatment, increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652699	Binding	Binding affinity to human TRIM4 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

4 cell lines: 3 cancer cell line, 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_D9UZ	Ubigene HEK293 TRIM4 KO	Transformed cell line	Female
CVCL_E0S8	Ubigene HeLa TRIM4 KO	Cancer cell line	Female
CVCL_TU21	HAP1 TRIM4 (-) 1	Cancer cell line	Male
CVCL_TU22	HAP1 TRIM4 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.