TRIM43B

Summary

TRIM43B (tripartite motif containing 43B, HGNC:37146) is a protein-coding gene on chromosome 2q11.1, encoding Tripartite motif-containing protein 43B (A6NCK2).

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in innate immune response and regulation of gene expression. Predicted to be active in cytoplasm.

Source: NCBI Gene 653192 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 7 total
• MANE Select transcript: NM_001164464

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:37146
Approved symbol	TRIM43B
Name	tripartite motif containing 43B
Location	2q11.1
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000144010
Ensembl biotype	protein_coding
Entrez	653192

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000639673

RefSeq mRNA: 1 — MANE Select: NM_001164464 NM_001164464

CCDS: CCDS92808

Canonical transcript exons

ENST00000639673 — 7 exons

Exon	Start	End
ENSE00002437137	95477008	95477645
ENSE00003484515	95480305	95480535
ENSE00003505323	95481595	95481690
ENSE00003552540	95478760	95478857
ENSE00003664716	95479386	95479408
ENSE00003804478	95484606	95484754
ENSE00003808283	95482304	95482718

Expression profiles

Bgee: expression breadth tissue_specific, 8 present calls, max score 85.60.

Top tissues by expression

120 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	85.60	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	81.00	gold quality
ganglionic eminence	UBERON:0004023	39.57	silver quality
colonic epithelium	UBERON:0000397	37.20	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
hindlimb stylopod muscle	UBERON:0004252	36.39	gold quality
bone marrow cell	CL:0002092	36.16	gold quality
apex of heart	UBERON:0002098	36.11	gold quality
skeletal muscle tissue	UBERON:0001134	35.40	gold quality
sural nerve	UBERON:0015488	35.22	gold quality
muscle tissue	UBERON:0002385	33.79	gold quality
bone marrow	UBERON:0002371	31.74	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	29.91	gold quality
stromal cell of endometrium	CL:0002255	29.87	gold quality
prefrontal cortex	UBERON:0000451	29.37	gold quality
liver	UBERON:0002107	28.69	gold quality
cerebellum	UBERON:0002037	28.64	gold quality
monocyte	CL:0000576	28.61	gold quality
cerebellar hemisphere	UBERON:0002245	28.60	gold quality
cerebellar cortex	UBERON:0002129	28.59	gold quality
leukocyte	CL:0000738	28.51	gold quality
duodenum	UBERON:0002114	28.14	gold quality
smooth muscle tissue	UBERON:0001135	28.09	gold quality
islet of Langerhans	UBERON:0000006	28.01	gold quality
right hemisphere of cerebellum	UBERON:0014890	27.95	gold quality
calcaneal tendon	UBERON:0003701	27.92	gold quality
lymph node	UBERON:0000029	27.57	gold quality
placenta	UBERON:0001987	27.45	gold quality
tonsil	UBERON:0002372	27.05	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.30

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

19 targeting TRIM43B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3662	99.99	73.82	5684
HSA-LET-7C-3P	99.95	73.42	2862
HSA-MIR-95-5P	99.89	72.17	3973
HSA-MIR-6844	99.82	70.69	2423
HSA-MIR-8076	99.78	68.52	1170
HSA-MIR-548AV-5P	99.60	70.84	2107
HSA-MIR-548K	99.60	70.84	2107
HSA-MIR-3616-5P	99.55	67.02	989
HSA-MIR-573	99.55	67.44	955
HSA-MIR-8054	99.48	70.81	2084
HSA-MIR-4293	99.22	65.46	1263
HSA-MIR-6780B-3P	99.13	67.18	622
HSA-MIR-3117-5P	99.04	67.93	618
HSA-MIR-4703-5P	98.53	70.13	1645
HSA-MIR-3942-5P	98.52	69.51	1517
HSA-MIR-4709-5P	98.51	67.25	1335
HSA-MIR-4766-3P	98.48	67.94	1347
HSA-MIR-10398-5P	97.12	64.94	1051
HSA-MIR-6514-5P	95.07	66.02	655

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
mus_musculus	Trim43c	ENSMUSG00000067399
mus_musculus	Trim43b	ENSMUSG00000079162
mus_musculus	Trim43a	ENSMUSG00000090693
rattus_norvegicus	Trim43a	ENSRNOG00000010699

Paralogs (80): MID2 (ENSG00000080561), TRIM9 (ENSG00000100505), MID1 (ENSG00000101871), MEFV (ENSG00000103313), TRIM35 (ENSG00000104228), TRIM14 (ENSG00000106785), TRIM37 (ENSG00000108395), TRIM2 (ENSG00000109654), TRIM3 (ENSG00000110171), TRIM38 (ENSG00000112343), TRIM62 (ENSG00000116525), TRIM67 (ENSG00000119283), TRIM32 (ENSG00000119401), BSPRY (ENSG00000119411), TRIM25 (ENSG00000121060), TRIM6 (ENSG00000121236), TRIM24 (ENSG00000122779), TRIM51 (ENSG00000124900), TRIM28 (ENSG00000130726), TRIM21 (ENSG00000132109), TRIM5 (ENSG00000132256), TRIM22 (ENSG00000132274), TRIM47 (ENSG00000132481), TRIM45 (ENSG00000134253), TRIM29 (ENSG00000137699), TRIM54 (ENSG00000138100), PML (ENSG00000140464), TRIM65 (ENSG00000141569), TRIM43 (ENSG00000144015), TRIM7 (ENSG00000146054), TRIM41 (ENSG00000146063), TRIM50 (ENSG00000146755), TRIM4 (ENSG00000146833), TRIM55 (ENSG00000147573), TRIM48 (ENSG00000150244), TRIM36 (ENSG00000152503), TRIM11 (ENSG00000154370), TRIM74 (ENSG00000155428), TRIM42 (ENSG00000155890), TRIM63 (ENSG00000158022)

Protein

Protein identifiers

Tripartite motif-containing protein 43B — A6NCK2 (reviewed: A6NCK2)

All UniProt accessions (1): A6NCK2

UniProt curated annotations — full annotation on UniProt →

Similarity. Belongs to the TRIM/RBCC family.

RefSeq proteins (1): NP_001157936* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000315	Znf_B-box	Domain
IPR001841	Znf_RING	Domain
IPR001870	B30.2/SPRY	Domain
IPR003877	SPRY_dom	Domain
IPR003879	Butyrophylin_SPRY	Domain
IPR013083	Znf_RING/FYVE/PHD	Homologous_superfamily
IPR013320	ConA-like_dom_sf	Homologous_superfamily
IPR017907	Znf_RING_CS	Conserved_site
IPR043136	B30.2/SPRY_sf	Homologous_superfamily
IPR050143	TRIM/RBCC	Family

Pfam: PF00622, PF00643, PF15227

UniProt features (10 total): binding site 4, zinc finger region 2, coiled-coil region 2, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A6NCK2-F1	88.47	0.64

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 93; 96; 115; 121

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 15 (showing top): GOBP_DEFENSE_RESPONSE_TO_OTHER_ORGANISM, GOMF_ACYLTRANSFERASE_ACTIVITY, GOMF_AMINOACYLTRANSFERASE_ACTIVITY, GOMF_UBIQUITIN_LIKE_PROTEIN_LIGASE_ACTIVITY, chr2q11, ESC_V6.5_UP_EARLY.V1_UP, ESC_V6.5_UP_LATE.V1_UP, LET_7C_3P, MIR548AV_5P_MIR548K, MIR8054, MIR4703_5P, MIR3942_5P, MIR4766_3P, MIR6780B_3P, GOBP_BIOLOGICAL_PROCESS_INVOLVED_IN_INTERSPECIES_INTERACTION_BETWEEN_ORGANISMS

GO Biological Process (2): regulation of gene expression (GO:0010468), innate immune response (GO:0045087)

GO Molecular Function (4): zinc ion binding (GO:0008270), ubiquitin protein ligase activity (GO:0061630), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): cytoplasm (GO:0005737)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
gene expression	1
regulation of macromolecule biosynthetic process	1
immune response	1
defense response to symbiont	1
transition metal ion binding	1
ubiquitin-protein transferase activity	1
ubiquitin-like protein ligase activity	1
binding	1
cation binding	1
intracellular anatomical structure	1
cellular anatomical structure	1

Protein interactions and networks

STRING

150 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TRIM43B	PRAMEF25	A6NGN4	507
TRIM43B	A0A096LNW4	A0A096LNW4	480
TRIM43B	PRAMEF15	P0DUQ1	478
TRIM43B	MBD3L3	A6NE82	447
TRIM43B	KCTD1	Q719H9	431
TRIM43B	MBD3L5	A6NJ08	418
TRIM43B	KHDC1L	Q5JSQ8	394
TRIM43B	PTPRR	Q15256	348
TRIM43B	ZSCAN4	Q8NAM6	323
TRIM43B	LEUTX	A8MZ59	323
TRIM43B	AK8	Q96MA6	322
TRIM43B	ZNF407	Q9C0G0	314
TRIM43B	KHDC1	Q4VXA5	307
TRIM43B	MBD3L2	Q8NHZ7	285
TRIM43B	DUX4L2	P0CJ85	272

IntAct

0 interactions, top by confidence:

BioGRID (3): TRIM43B (Affinity Capture-MS), SSR3 (Cross-Linking-MS (XL-MS)), PARP1 (Cross-Linking-MS (XL-MS))

ESM2 similar proteins: A0A3B3IT33, A6NCK2, A6NDI0, A6NGJ6, A6NI03, A6NLI5, C9J1S8, I1YAP6, K7N6K2, P0CI25, P0CI26, P15533, Q0PF16, Q1ACD5, Q1ACD6, Q1ACD7, Q1ACD8, Q2YEM8, Q2YEM9, Q2YEN0, Q2YEN2, Q3ZEE5, Q587N6, Q587N7, Q5BN31, Q5C8T6, Q5C8T8, Q5C8U1, Q5C8U3, Q5C8U4, Q5D7H7, Q5D7H8, Q5D7I0, Q5D7I1, Q5D7I2, Q5D7I3, Q5D7I5, Q5D7I6, Q5D7I9, Q5D7J0

Diamond homologs: A0A3B3IT33, A6NCK2, A6NDI0, A6NGJ6, A6NI03, A6NLI5, B0BLU1, C9J1S8, I1YAP6, O00478, O00481, O15344, O75677, O75678, O75679, O76064, P0CI25, P0CI26, P18892, P19474, P62603, P86448, P86449, Q13410, Q2HJ46, Q3C1V9, Q3TL54, Q4KLN8, Q5EBN2, Q5PQN2, Q5R4I2, Q5R996, Q61510, Q62556, Q6INS5, Q6MFY8, Q6UX41, Q6UXE8, Q6ZWI9, Q7T308

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
Ub:E2	“up-regulates activity”	TRIM43B	ubiquitination

Disease & clinical

Clinical variants and AI predictions

ClinVar

7 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	5
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

3004 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
2:95477257:A:C	F416L	0.978
2:95477257:A:T	F416L	0.978
2:95477259:A:G	F416L	0.978
2:95477460:A:G	W349R	0.961
2:95477460:A:T	W349R	0.961
2:95477490:A:G	W339R	0.956
2:95477490:A:T	W339R	0.956
2:95477258:A:G	F416S	0.947
2:95477488:C:A	W339C	0.947
2:95477488:C:G	W339C	0.947
2:95482613:G:C	F34L	0.946
2:95482613:G:T	F34L	0.946
2:95482615:A:G	F34L	0.946
2:95478764:G:C	F285L	0.943
2:95478764:G:T	F285L	0.943
2:95478766:A:G	F285L	0.943
2:95477287:A:C	F406L	0.941
2:95477287:A:T	F406L	0.941
2:95477289:A:G	F406L	0.941
2:95477185:G:C	F440L	0.932
2:95477185:G:T	F440L	0.932
2:95477187:A:G	F440L	0.932
2:95477450:C:T	G352E	0.928
2:95477457:C:G	A350P	0.925
2:95482406:G:C	F103L	0.923
2:95482406:G:T	F103L	0.923
2:95482408:A:G	F103L	0.923
2:95477451:C:G	G352R	0.907
2:95477451:C:T	G352R	0.907
2:95477458:C:A	W349C	0.904

dbSNP variants (sampled 300 via entrez): RS1000033358 (2:95482061 G>A), RS1000933828 (2:95486071 C>T), RS1000978434 (2:95486226 G>A,C), RS1000987048 (2:95480191 A>G), RS1002330513 (2:95484101 T>G), RS1002984883 (2:95480038 A>G), RS1003374182 (2:95484348 A>G), RS1004046934 (2:95486716 G>A,T), RS1005001503 (2:95485395 G>A,T), RS1005054303 (2:95485701 G>A), RS1006352568 (2:95483072 T>C), RS1006553526 (2:95485072 C>A,T), RS1007968057 (2:95483931 A>C,G), RS1008795735 (2:95481015 A>G), RS1010412935 (2:95482199 G>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

6 total (human), top 6 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
methylmercuric chloride	increases expression	1
ethyl-p-hydroxybenzoate	increases expression	1
Benzo(a)pyrene	affects methylation, increases methylation	1
Estradiol	decreases expression	1
Thiram	increases expression	1
Genistein	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.