TRIM64B

Summary

TRIM64B (tripartite motif containing 64B, HGNC:37147) is a protein-coding gene on chromosome 11q14.3, encoding Tripartite motif-containing protein 64B (A6NI03).

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in innate immune response and regulation of gene expression. Predicted to be active in cytoplasm.

Source: NCBI Gene 642446 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 96 total
• MANE Select transcript: NM_001164397

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:37147
Approved symbol	TRIM64B
Name	tripartite motif containing 64B
Location	11q14.3
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000189253
Ensembl biotype	protein_coding
Entrez	642446

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000329862

RefSeq mRNA: 1 — MANE Select: NM_001164397 NM_001164397

CCDS: CCDS53693

Canonical transcript exons

ENST00000329862 — 7 exons

Exon	Start	End
ENSE00002435013	89872215	89872312
ENSE00002437643	89870438	89871114
ENSE00002459320	89874978	89875073
ENSE00002464660	89874049	89874279
ENSE00002464799	89875610	89876021
ENSE00002520282	89873268	89873290
ENSE00003966869	89878372	89878487

Expression profiles

Bgee: expression breadth tissue_specific, 8 present calls, max score 64.84.

Top tissues by expression

132 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
placenta	UBERON:0001987	64.84	gold quality
monocyte	CL:0000576	49.11	gold quality
leukocyte	CL:0000738	47.17	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	45.51	gold quality
bone marrow cell	CL:0002092	38.40	gold quality
colonic epithelium	UBERON:0000397	37.20	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
ganglionic eminence	UBERON:0004023	35.49	gold quality
apex of heart	UBERON:0002098	34.94	gold quality
cortex of kidney	UBERON:0001225	33.96	gold quality
skeletal muscle tissue	UBERON:0001134	33.38	gold quality
liver	UBERON:0002107	33.12	gold quality
bone marrow	UBERON:0002371	32.89	gold quality
blood	UBERON:0000178	32.44	silver quality
muscle tissue	UBERON:0002385	32.19	gold quality
hindlimb stylopod muscle	UBERON:0004252	32.15	gold quality
right lobe of liver	UBERON:0001114	31.61	gold quality
prefrontal cortex	UBERON:0000451	31.24	gold quality
sural nerve	UBERON:0015488	30.93	gold quality
rectum	UBERON:0001052	30.62	gold quality
lymph node	UBERON:0000029	29.89	gold quality
stromal cell of endometrium	CL:0002255	29.87	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	29.01	gold quality
endometrium	UBERON:0001295	28.79	gold quality
islet of Langerhans	UBERON:0000006	28.67	gold quality
gall bladder	UBERON:0002110	28.46	gold quality
duodenum	UBERON:0002114	28.14	gold quality
metanephros cortex	UBERON:0010533	28.08	gold quality
frontal cortex	UBERON:0001870	27.67	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.28

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

17 targeting TRIM64B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4262	100.00	73.26	3931
HSA-MIR-181A-5P	99.99	72.96	2995
HSA-MIR-181B-5P	99.99	72.97	2996
HSA-MIR-181C-5P	99.99	72.95	2996
HSA-MIR-181D-5P	99.99	73.04	2997
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-548AV-5P	99.60	70.84	2107
HSA-MIR-548K	99.60	70.84	2107
HSA-MIR-4761-5P	99.51	66.69	804
HSA-MIR-8054	99.48	70.81	2084
HSA-MIR-6799-5P	99.14	65.72	2093
HSA-MIR-4774-3P	98.90	67.82	737
HSA-MIR-5581-3P	98.55	70.31	1161
HSA-MIR-299-3P	97.73	66.67	773
HSA-MIR-4670-3P	97.37	68.35	1378
HSA-MIR-3152-5P	96.98	66.88	819
HSA-MIR-136-3P	93.27	66.31	131

Cross-species orthologs

0 orthologs

Paralogs (80): MID2 (ENSG00000080561), TRIM9 (ENSG00000100505), MID1 (ENSG00000101871), MEFV (ENSG00000103313), TRIM35 (ENSG00000104228), TRIM14 (ENSG00000106785), TRIM37 (ENSG00000108395), TRIM2 (ENSG00000109654), TRIM3 (ENSG00000110171), TRIM38 (ENSG00000112343), TRIM62 (ENSG00000116525), TRIM67 (ENSG00000119283), TRIM32 (ENSG00000119401), BSPRY (ENSG00000119411), TRIM25 (ENSG00000121060), TRIM6 (ENSG00000121236), TRIM24 (ENSG00000122779), TRIM51 (ENSG00000124900), TRIM28 (ENSG00000130726), TRIM21 (ENSG00000132109), TRIM5 (ENSG00000132256), TRIM22 (ENSG00000132274), TRIM47 (ENSG00000132481), TRIM45 (ENSG00000134253), TRIM29 (ENSG00000137699), TRIM54 (ENSG00000138100), PML (ENSG00000140464), TRIM65 (ENSG00000141569), TRIM43B (ENSG00000144010), TRIM43 (ENSG00000144015), TRIM7 (ENSG00000146054), TRIM41 (ENSG00000146063), TRIM50 (ENSG00000146755), TRIM4 (ENSG00000146833), TRIM55 (ENSG00000147573), TRIM48 (ENSG00000150244), TRIM36 (ENSG00000152503), TRIM11 (ENSG00000154370), TRIM74 (ENSG00000155428), TRIM42 (ENSG00000155890)

Protein

Protein identifiers

Tripartite motif-containing protein 64B — A6NI03 (reviewed: A6NI03)

All UniProt accessions (1): A6NI03

UniProt curated annotations — full annotation on UniProt →

Similarity. Belongs to the TRIM/RBCC family.

RefSeq proteins (1): NP_001157869* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000315	Znf_B-box	Domain
IPR001841	Znf_RING	Domain
IPR001870	B30.2/SPRY	Domain
IPR003877	SPRY_dom	Domain
IPR003879	Butyrophylin_SPRY	Domain
IPR013083	Znf_RING/FYVE/PHD	Homologous_superfamily
IPR013320	ConA-like_dom_sf	Homologous_superfamily
IPR017907	Znf_RING_CS	Conserved_site
IPR018957	Znf_C3HC4_RING-type	Domain
IPR043136	B30.2/SPRY_sf	Homologous_superfamily
IPR050143	TRIM/RBCC	Family

Pfam: PF00097, PF00622, PF00643

UniProt features (9 total): binding site 4, zinc finger region 2, chain 1, domain 1, coiled-coil region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A6NI03-F1	85.53	0.57

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 92; 95; 114; 120

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 7 (showing top): GOBP_DEFENSE_RESPONSE_TO_OTHER_ORGANISM, GOMF_ACYLTRANSFERASE_ACTIVITY, GOMF_AMINOACYLTRANSFERASE_ACTIVITY, GOMF_UBIQUITIN_LIKE_PROTEIN_LIGASE_ACTIVITY, MIR3152_5P, chr11q14, GOBP_BIOLOGICAL_PROCESS_INVOLVED_IN_INTERSPECIES_INTERACTION_BETWEEN_ORGANISMS

GO Biological Process (2): regulation of gene expression (GO:0010468), innate immune response (GO:0045087)

GO Molecular Function (4): zinc ion binding (GO:0008270), ubiquitin protein ligase activity (GO:0061630), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (1): cytoplasm (GO:0005737)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
gene expression	1
regulation of macromolecule biosynthetic process	1
immune response	1
defense response to symbiont	1
transition metal ion binding	1
ubiquitin-protein transferase activity	1
ubiquitin-like protein ligase activity	1
binding	1
cation binding	1
intracellular anatomical structure	1
cellular anatomical structure	1

Protein interactions and networks

STRING

180 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TRIM64B	PPIAL4D	F5H284	604
TRIM64B	OR5D16	Q8NGK9	599
TRIM64B	NAALAD2	Q9Y3Q0	509
TRIM64B	POTEM	A6NI47	477
TRIM64B	SPAG11A	Q6PDA7	449
TRIM64B	CCDC124	Q96CT7	434
TRIM64B	A0A0G2JN59	A0A0G2JN59	371
TRIM64B	APOBEC4	Q8WW27	320
TRIM64B	NSUN4	Q96CB9	300
TRIM64B	FOLH1	Q04609	288
TRIM64B	MYO3A	Q8NEV4	288
TRIM64B	ACBD7	Q8N6N7	274
TRIM64B	TRIM44	Q96DX7	250
TRIM64B	RGPD3	A6NKT7	250
TRIM64B	TRIM66	O15016	249

IntAct

0 interactions, top by confidence:

ESM2 similar proteins: A0A3B3IT33, A6NCK2, A6NDI0, A6NGJ6, A6NI03, A6NLI5, C9J1S8, I1YAP6, K7N6K2, P0CI25, P0CI26, P15533, Q0PF16, Q1ACD5, Q1ACD6, Q1ACD7, Q1ACD8, Q2YEM8, Q2YEM9, Q2YEN0, Q2YEN2, Q3ZEE5, Q587N6, Q587N7, Q5BN31, Q5C8T6, Q5C8T8, Q5C8U1, Q5C8U3, Q5C8U4, Q5D7H7, Q5D7H8, Q5D7I0, Q5D7I1, Q5D7I2, Q5D7I3, Q5D7I5, Q5D7I6, Q5D7I9, Q5D7J0

Diamond homologs: A0A3B3IT33, A6NCK2, A6NDI0, A6NGJ6, A6NI03, A6NLI5, B0BLU1, C9J1S8, I1YAP6, O00478, O00481, O15344, O75677, O75678, O75679, O76064, P0CI25, P0CI26, P18892, P19474, P62603, P86448, P86449, Q13410, Q2HJ46, Q3C1V9, Q3TL54, Q4KLN8, Q5EBN2, Q5PQN2, Q5R4I2, Q5R996, Q61510, Q62556, Q6INS5, Q6MFY8, Q6UX41, Q6UXE8, Q6ZWI9, Q7T308

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
Ub:E2	“up-regulates activity”	TRIM64B	ubiquitination

Disease & clinical

Clinical variants and AI predictions

ClinVar

96 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	94
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

451 predictions. Top by Δscore:

Variant	Effect	Δscore
11:89871115:C:CC	acceptor_gain	1.0000
11:89872209:TCTTA:T	donor_loss	1.0000
11:89872210:CTTA:C	donor_loss	1.0000
11:89872211:TTA:T	donor_loss	1.0000
11:89872212:TAC:T	donor_loss	1.0000
11:89872213:A:AC	donor_gain	1.0000
11:89872213:A:AG	donor_loss	1.0000
11:89872214:C:CC	donor_gain	1.0000
11:89872214:CCT:C	donor_gain	1.0000
11:89872308:CAGTC:C	acceptor_gain	1.0000
11:89872309:AGTC:A	acceptor_gain	1.0000
11:89872310:GTC:G	acceptor_gain	1.0000
11:89872311:TC:T	acceptor_gain	1.0000
11:89872312:CC:C	acceptor_gain	1.0000
11:89872312:CCT:C	acceptor_loss	1.0000
11:89872313:C:CA	acceptor_loss	1.0000
11:89872313:C:CC	acceptor_gain	1.0000
11:89872314:T:C	acceptor_loss	1.0000
11:89873264:TCA:T	donor_loss	1.0000
11:89873265:CACCT:C	donor_loss	1.0000
11:89873267:CCTTG:C	donor_gain	1.0000
11:89873288:ATCC:A	acceptor_loss	1.0000
11:89873289:TCCT:T	acceptor_loss	1.0000
11:89873290:CCTGC:C	acceptor_loss	1.0000
11:89873291:C:CA	acceptor_loss	1.0000
11:89873291:C:CC	acceptor_gain	1.0000
11:89873292:T:A	acceptor_loss	1.0000
11:89874972:TCTTA:T	donor_loss	1.0000
11:89874973:CTTAC:C	donor_loss	1.0000
11:89874974:TTA:T	donor_loss	1.0000

AlphaMissense

2999 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
11:89870714:A:C	F419L	0.971
11:89870714:A:T	F419L	0.971
11:89870716:A:G	F419L	0.971
11:89870917:A:G	W352R	0.962
11:89870917:A:T	W352R	0.962
11:89870744:A:C	F409L	0.960
11:89870744:A:T	F409L	0.960
11:89870746:A:G	F409L	0.960
11:89875916:A:C	F34L	0.960
11:89875916:A:T	F34L	0.960
11:89875918:A:G	F34L	0.960
11:89875712:G:C	F102L	0.958
11:89875712:G:T	F102L	0.958
11:89875714:A:G	F102L	0.958
11:89870945:C:A	W342C	0.956
11:89870945:C:G	W342C	0.956
11:89870947:A:G	W342R	0.949
11:89870947:A:T	W342R	0.949
11:89872219:G:C	F284L	0.942
11:89872219:G:T	F284L	0.942
11:89872221:A:G	F284L	0.942
11:89870752:C:A	G407W	0.936
11:89870966:G:C	F335L	0.934
11:89870966:G:T	F335L	0.934
11:89870968:A:G	F335L	0.934
11:89870751:C:T	G407E	0.933
11:89870715:A:G	F419S	0.928
11:89870907:C:T	G355E	0.928
11:89870678:A:C	F431L	0.924
11:89870678:A:T	F431L	0.924

dbSNP variants (sampled 300 via entrez): RS1003924453 (11:89872947 T>A,C), RS1004331709 (11:89878236 A>G), RS1014212930 (11:89873763 C>T), RS1019781306 (11:89878322 T>C), RS1022794771 (11:89876590 T>C), RS1023759030 (11:89876302 G>T), RS1023896608 (11:89873808 G>A), RS1027081546 (11:89876482 G>A), RS1027195962 (11:89878038 C>T), RS1028299142 (11:89878576 G>C,T), RS1030661707 (11:89873302 T>C), RS1030713910 (11:89871515 T>C,G), RS1031981625 (11:89876742 T>A,C,G), RS1035055556 (11:89872951 A>C), RS1040661783 (11:89874290 G>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

2 total (human), top 2 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	decreases methylation	1
Okadaic Acid	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.