TRIM66

Summary

TRIM66 (tripartite motif containing 66, HGNC:29005) is a protein-coding gene on chromosome 11p15.4, encoding Tripartite motif-containing protein 66 (O15016). May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity.

Predicted to enable chromatin binding activity; identical protein binding activity; and zinc ion binding activity. Predicted to act upstream of or within negative regulation of DNA-templated transcription. Located in nucleus.

Source: NCBI Gene 9866 — RefSeq curated summary.

At a glance

• GWAS associations: 32
• Clinical variants (ClinVar): 219 total
• Druggable target: yes
• MANE Select transcript: NM_001388022

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29005
Approved symbol	TRIM66
Name	tripartite motif containing 66
Location	11p15.4
Locus type	gene with protein product
Status	Approved
Aliases	KIAA0298, TIF1D
Ensembl gene	ENSG00000166436
Ensembl biotype	protein_coding
OMIM	612000
Entrez	9866

Gene structure

Transcript identifiers

Ensembl transcripts: 13 — 6 retained_intron, 6 protein_coding, 1 nonsense_mediated_decay

ENST00000481014, ENST00000525788, ENST00000529057, ENST00000529211, ENST00000530502, ENST00000531498, ENST00000644261, ENST00000646038, ENST00000705689, ENST00000705690, ENST00000705691, ENST00000705692, ENST00000705693

RefSeq mRNA: 4 — MANE Select: NM_001388022 NM_001388022, NM_001388023, NM_001388024, NM_014818

CCDS: CCDS91432, CCDS91433

Canonical transcript exons

ENST00000646038 — 25 exons

Exon	Start	End
ENSE00002142854	8621032	8621321
ENSE00003527444	8620446	8620572
ENSE00003635054	8620050	8620124
ENSE00003674670	8619383	8619535
ENSE00003693907	8618750	8618968
ENSE00003816862	8674806	8674883
ENSE00003817895	8672248	8672385
ENSE00003821373	8679901	8680063
ENSE00003822868	8612040	8618003
ENSE00003825889	8651800	8651903
ENSE00003827837	8679620	8679814
ENSE00003993999	8622816	8622876
ENSE00003994000	8645741	8645887
ENSE00003994003	8621645	8621819
ENSE00003994006	8624359	8624551
ENSE00003994009	8648416	8648548
ENSE00003994011	8671786	8672098
ENSE00003994014	8624713	8625228
ENSE00003994016	8646447	8646561
ENSE00003994021	8647970	8648086
ENSE00003994022	8643009	8643126
ENSE00003994025	8638654	8638815
ENSE00003994026	8640227	8641152
ENSE00003994028	8682601	8682665
ENSE00003994030	8649740	8649887

Expression profiles

Bgee: expression breadth ubiquitous, 227 present calls, max score 90.74.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 8.2877 / max 78.6808, expressed in 1735 samples.

FANTOM5 promoters (8 alternative TSS)

Promoter ID	TPM avg	Samples expressed
118508	7.3406	1719
118509	0.6498	363
118507	0.1691	72
118505	0.0848	28
118504	0.0185	5
118506	0.0129	3
118503	0.0071	2
118501	0.0049	1

Top tissues by expression

282 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	90.74	gold quality
right testis	UBERON:0004534	90.54	gold quality
left testis	UBERON:0004533	90.22	gold quality
testis	UBERON:0000473	87.24	gold quality
calcaneal tendon	UBERON:0003701	84.38	gold quality
corpus epididymis	UBERON:0004359	82.78	gold quality
body of pancreas	UBERON:0001150	82.02	gold quality
right lobe of liver	UBERON:0001114	81.51	gold quality
tendon	UBERON:0000043	80.98	gold quality
pancreatic ductal cell	CL:0002079	80.23	silver quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	79.89	gold quality
right uterine tube	UBERON:0001302	79.86	gold quality
pituitary gland	UBERON:0000007	79.33	gold quality
right adrenal gland	UBERON:0001233	79.31	gold quality
cauda epididymis	UBERON:0004360	79.17	gold quality
right adrenal gland cortex	UBERON:0035827	79.16	gold quality
pancreas	UBERON:0001264	78.57	gold quality
skin of leg	UBERON:0001511	78.31	gold quality
left adrenal gland	UBERON:0001234	77.81	gold quality
left adrenal gland cortex	UBERON:0035825	77.64	gold quality
caput epididymis	UBERON:0004358	77.62	gold quality
adenohypophysis	UBERON:0002196	77.56	gold quality
adrenal cortex	UBERON:0001235	77.51	gold quality
skin of abdomen	UBERON:0001416	77.40	gold quality
stromal cell of endometrium	CL:0002255	77.36	gold quality
body of stomach	UBERON:0001161	77.28	gold quality
descending thoracic aorta	UBERON:0002345	77.16	gold quality
right hemisphere of cerebellum	UBERON:0014890	77.16	gold quality
right lobe of thyroid gland	UBERON:0001119	76.99	gold quality
adrenal gland	UBERON:0002369	76.98	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	6.19

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

319 targeting TRIM66, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4668-3P	100.00	68.74	2635
HSA-MIR-7110-3P	100.00	73.18	2486
HSA-MIR-9-5P	100.00	72.28	2361
HSA-MIR-4476	100.00	68.18	2030
HSA-MIR-6876-5P	100.00	67.68	2126
HSA-MIR-513A-5P	100.00	69.77	2465
HSA-MIR-4673	100.00	66.64	1490
HSA-MIR-4533	100.00	69.48	2758
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-6758-5P	100.00	66.21	1470
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-6856-5P	100.00	65.47	1298
HSA-MIR-548AW	99.99	72.57	3559
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-4531	99.99	69.70	3181
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-511-3P	99.99	68.85	1467
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-373-5P	99.98	75.36	4753
HSA-MIR-616-5P	99.98	75.58	4775
HSA-MIR-4645-5P	99.98	65.81	1284
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-3617-3P	99.98	67.86	918
HSA-MIR-1229-3P	99.97	66.49	906
HSA-MIR-512-3P	99.97	67.35	1049
HSA-MIR-6825-5P	99.96	69.81	3431
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-3605-5P	99.96	67.12	932

Literature-anchored findings (GeneRIF, showing 14)

• Data suggest a role for transcriptional intermediary factor 1 (TIF1) delta in heterochromatin-mediated gene silencing during postmeiotic phases of spermatogenesis. (PMID:15322135)
• TRIM66 may act as an oncogene through suppressing apoptosis pathway and promoting TGF-beta signaling in osteosarcoma carcinogenesis. TRIM66 may be a prognostic factor and potential therapeutic target in osteosarcoma. (PMID:26247633)
• TRIMM66 was highly expressed in NSCLC tissues compared with normal paracancerous tissues (P= 0.001). The high TRIM66 expression closely associated with lymph node metastasis and TNM stage in NSCLC patients (P< 0.05). (PMID:28946563)
• Silence TRIM66 expression suppressed non-small cell lung cancer cell proliferation, invasion, and migration (PMID:29929749)
• TRIM66 functioned as an oncogenic protein in HCC by promoting the activation of Wnt/beta-catenin signaling (PMID:30710548)
• High TRIM66 expression is associated with cell proliferation, migration and invasion in colorectal cancer through JAK2/STAT3 pathway. (PMID:31472144)
• TRIM66 recruits Sirt6 to deacetylate H3K56ac, negatively regulating the level of H3K56ac and facilitating the initiation of DNA damage repair. Importantly, Trim66-deficient blastocysts also exhibit higher levels of H3K56ac and DNA damage. (PMID:31537782)
• TRIM66 promotes malignant progression of prostate carcinoma through the JAK/STAT pathway. (PMID:31981447)
• Association of the SNP rs112369934 near TRIM66 Gene with POAG Endophenotypes in African Americans. (PMID:34573402)
• Zhoushi Qi Ling decoction represses docetaxel resistance and glycolysis of castration-resistant prostate cancer via regulation of SNHG10/miR-1271-5p/TRIM66 axis. (PMID:34613933)
• Knockdown of TRIM66 in MDA-MB-468 triple negative breast cancer cell line suppresses proliferation and promotes apoptosis through EGFR signaling. (PMID:34706523)
• TRIM66 hastens the malignant progression of non-small cell lung cancer via modulating MMP9-mediated TGF-beta/SMAD pathway. (PMID:35301175)
• TRIM66 Promotes Malignant Progression of Non-Small-Cell Lung Cancer Cells via Targeting MMP9. (PMID:35912155)
• Circ-SATB2 (hsa_circ_0008928) and miR-150-5p are regulators of TRIM66 in the regulation of NSCLC cell growth and metastasis of NSCLC cells via the ceRNA pathway. (PMID:38084627)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	trim66	ENSDARG00000054332
mus_musculus	Trim66	ENSMUSG00000031026
rattus_norvegicus	Trim66	ENSRNOG00000014373

Paralogs (80): MID2 (ENSG00000080561), TRIM9 (ENSG00000100505), MID1 (ENSG00000101871), MEFV (ENSG00000103313), TRIM35 (ENSG00000104228), TRIM14 (ENSG00000106785), TRIM37 (ENSG00000108395), TRIM2 (ENSG00000109654), TRIM3 (ENSG00000110171), TRIM38 (ENSG00000112343), TRIM62 (ENSG00000116525), TRIM67 (ENSG00000119283), TRIM32 (ENSG00000119401), BSPRY (ENSG00000119411), TRIM25 (ENSG00000121060), TRIM6 (ENSG00000121236), TRIM24 (ENSG00000122779), TRIM51 (ENSG00000124900), TRIM28 (ENSG00000130726), TRIM21 (ENSG00000132109), TRIM5 (ENSG00000132256), TRIM22 (ENSG00000132274), TRIM47 (ENSG00000132481), TRIM45 (ENSG00000134253), TRIM29 (ENSG00000137699), TRIM54 (ENSG00000138100), PML (ENSG00000140464), TRIM65 (ENSG00000141569), TRIM43B (ENSG00000144010), TRIM43 (ENSG00000144015), TRIM7 (ENSG00000146054), TRIM41 (ENSG00000146063), TRIM50 (ENSG00000146755), TRIM4 (ENSG00000146833), TRIM55 (ENSG00000147573), TRIM48 (ENSG00000150244), TRIM36 (ENSG00000152503), TRIM11 (ENSG00000154370), TRIM74 (ENSG00000155428), TRIM42 (ENSG00000155890)

Protein

Protein identifiers

Tripartite motif-containing protein 66 — O15016 (reviewed: O15016)

All UniProt accessions (7): A0A2R8YE93, A0A8Z5E822, A0A994J572, A0A994J5L5, A0A994J7V7, B5MCJ9, H0YF52

UniProt curated annotations — full annotation on UniProt →

Function. May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association.

Subunit / interactions. Can form homodimers and heterodimers. Interacts with CBX5, CBX1 and CBX3 via PxVxL motif.

Subcellular location. Nucleus.

Isoforms (3)

UniProt ID	Names	Canonical?
O15016-1	1	yes
O15016-2	2
O15016-3	3

RefSeq proteins (4): NP_001374951, NP_001374952, NP_001374953, NP_055633 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000315	Znf_B-box	Domain
IPR001487	Bromodomain	Domain
IPR001965	Znf_PHD	Domain
IPR003649	Bbox_C	Domain
IPR011011	Znf_FYVE_PHD	Homologous_superfamily
IPR013083	Znf_RING/FYVE/PHD	Homologous_superfamily
IPR019786	Zinc_finger_PHD-type_CS	Conserved_site
IPR019787	Znf_PHD-finger	Domain
IPR036427	Bromodomain-like_sf	Homologous_superfamily
IPR037372	TRIM66_Bbox1_Znf	Domain

Pfam: PF00439, PF00628, PF00643, PF25287

UniProt features (41 total): binding site 8, helix 7, splice variant 5, region of interest 5, strand 4, compositionally biased region 3, zinc finger region 3, turn 2, chain 1, domain 1, coiled-coil region 1, short sequence motif 1

Structure

Experimental structures (PDB)

3 structures.

PDB	Method	Resolution (Å)
6IEU	X-RAY DIFFRACTION	1.79
8JZW	X-RAY DIFFRACTION	1.8
6IET	X-RAY DIFFRACTION	2.1

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O15016-F1	55.38	0.16

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (8): 109; 112; 133; 139; 169; 172; 192; 197

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 98 (showing top): GAZIN_EPIGENETIC_SILENCING_BY_KRAS, MORF_TNFRSF25, PURBEY_TARGETS_OF_CTBP1_NOT_SATB1_UP, PEDRIOLI_MIR31_TARGETS_DN, ATF6_TARGET_GENES, MIR651_3P, MIR548P, MIR548AW, MIR4306, MIR15A_5P, MIR195_5P, MIR15B_5P, MIR16_5P, MIR7110_3P, MIR6838_5P

GO Biological Process (1): negative regulation of DNA-templated transcription (GO:0045892)

GO Molecular Function (5): zinc ion binding (GO:0008270), chromatin binding (GO:0003682), protein binding (GO:0005515), identical protein binding (GO:0042802), metal ion binding (GO:0046872)

GO Cellular Component (3): chromatin (GO:0000785), nucleus (GO:0005634), chromocenter (GO:0010369)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
binding	2
DNA-templated transcription	1
regulation of DNA-templated transcription	1
negative regulation of RNA biosynthetic process	1
transition metal ion binding	1
protein binding	1
cation binding	1
chromosome	1
cellular anatomical structure	1
intracellular membrane-bounded organelle	1
intracellular membraneless organelle	1

Protein interactions and networks

STRING

1004 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TRIM66	CBX3	Q13185	921
TRIM66	TRIM17	Q9Y577	786
TRIM66	BBOX1	O75936	748
TRIM66	TRIM47	Q96LD4	623
TRIM66	TRAT1	Q6PIZ9	622
TRIM66	TRIM25	Q14258	614
TRIM66	TRIM44	Q96DX7	593
TRIM66	TRIM11	Q96F44	587
TRIM66	TRIM28	Q13263	576
TRIM66	TRIM8	Q9BZR9	575
TRIM66	TATDN1	Q6P1N9	565
TRIM66	TRIM59	Q8IWR1	559
TRIM66	TRIM62	Q9BVG3	540
TRIM66	TRIM4	Q9C037	517
TRIM66	CBX1	P23197	515

IntAct

4 interactions, top by confidence:

A	B	Type	Score
NP	KPNA4	psi-mi:“MI:0914”(association)	0.350
NP	TRIM66	psi-mi:“MI:0914”(association)	0.350
DYRK1A	TRIM66	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (203): TRIM66 (Affinity Capture-MS), TRIM66 (Affinity Capture-MS), MMS19 (Affinity Capture-MS), CEP295 (Affinity Capture-MS), TP53 (Affinity Capture-MS), CEP170 (Affinity Capture-MS), LTN1 (Affinity Capture-MS), NUP188 (Affinity Capture-MS), MON2 (Affinity Capture-MS), RPTOR (Affinity Capture-MS), AKAP8 (Affinity Capture-MS), ZNF318 (Affinity Capture-MS), TTC37 (Affinity Capture-MS), MCM3AP (Affinity Capture-MS), FANCD2 (Affinity Capture-MS)

ESM2 similar proteins: A0A1B0GVQ3, A0A1W2PPK0, A0A1W2PPM1, A2A9I7, A6NCI8, A6QQS3, A7XCE8, E9PI22, E9PXT9, O15016, O91083, P09414, P0DMB1, P17923, P18804, P20879, P35965, P49750, Q0P670, Q12857, Q1RMX6, Q32LN6, Q32MG2, Q3B8N5, Q3T016, Q3V0A6, Q4JK59, Q5BI31, Q5T035, Q5ZKH6, Q642A3, Q6AXV6, Q6IMN6, Q6P1W5, Q6PEX7, Q6X4T0, Q80YD3, Q86UF4, Q8BII1, Q8C5V0

Diamond homologs: A0A0R4IXF6, A0A7U2QYM2, A2AHJ4, A2AUY4, A2BIL7, B2KF05, B2RWS6, B7ZS37, D4A7T3, E9Q2Z1, F1QW93, F1R5H6, F7DRV9, O15016, O15164, O60885, O88379, P21675, P25439, P25440, P34545, P45481, P51123, P87152, Q02206, Q03330, Q08D75, Q09472, Q12830, Q15059, Q1LUC3, Q32S26, Q338B9, Q3UQU0, Q4R8Y1, Q54BA2, Q54UW4, Q55C84, Q56R14, Q58F21

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

219 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	186
Likely benign	13
Benign	4

Top pathogenic / likely-pathogenic (0)

SpliceAI

4613 predictions. Top by Δscore:

Variant	Effect	Δscore
11:8619381:A:AC	donor_gain	1.0000
11:8619382:C:CC	donor_gain	1.0000
11:8619382:CATAA:C	donor_gain	1.0000
11:8619385:A:AC	donor_gain	1.0000
11:8619386:A:C	donor_gain	1.0000
11:8619395:AG:A	donor_gain	1.0000
11:8619396:G:C	donor_gain	1.0000
11:8619435:T:TA	donor_gain	1.0000
11:8619537:T:C	acceptor_gain	1.0000
11:8619551:A:C	acceptor_gain	1.0000
11:8620043:T:TA	donor_gain	1.0000
11:8620122:CTT:C	acceptor_gain	1.0000
11:8620443:TA:T	donor_loss	1.0000
11:8620444:A:AG	donor_loss	1.0000
11:8620445:CCT:C	donor_loss	1.0000
11:8620445:CCTT:C	donor_gain	1.0000
11:8620465:AGG:A	donor_gain	1.0000
11:8620568:CTCCC:C	acceptor_gain	1.0000
11:8620570:CCC:C	acceptor_gain	1.0000
11:8620571:CC:C	acceptor_gain	1.0000
11:8620571:CCC:C	acceptor_gain	1.0000
11:8620572:CC:C	acceptor_gain	1.0000
11:8620573:C:CC	acceptor_gain	1.0000
11:8621640:GGTAC:G	donor_loss	1.0000
11:8621641:GTAC:G	donor_loss	1.0000
11:8621642:TAC:T	donor_loss	1.0000
11:8621643:A:T	donor_loss	1.0000
11:8622873:CACT:C	acceptor_gain	1.0000
11:8622875:CT:C	acceptor_gain	1.0000
11:8622877:C:CC	acceptor_gain	1.0000

AlphaMissense

9184 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
11:8619389:G:C	F1122L	1.000
11:8619389:G:T	F1122L	1.000
11:8619391:A:G	F1122L	1.000
11:8640288:A:C	I551S	1.000
11:8640288:A:G	I551T	1.000
11:8640300:A:T	I547N	1.000
11:8646496:A:G	L158P	1.000
11:8646509:C:G	A154P	1.000
11:8618934:A:G	L1136P	0.999
11:8619390:A:G	F1122S	0.999
11:8621087:A:G	C988R	0.999
11:8621121:G:C	C976W	0.999
11:8621123:A:G	C976R	0.999
11:8621130:A:C	C973W	0.999
11:8621131:C:T	C973Y	0.999
11:8621132:A:G	C973R	0.999
11:8621688:A:G	L895P	0.999
11:8621733:A:G	F880S	0.999
11:8621772:A:G	L867P	0.999
11:8621781:A:G	L864P	0.999
11:8640279:A:C	I554S	0.999
11:8640279:A:T	I554N	0.999
11:8640288:A:T	I551N	0.999
11:8640291:T:G	Q550P	0.999
11:8640294:C:A	G549V	0.999
11:8640294:C:T	G549E	0.999
11:8640295:C:A	G549W	0.999
11:8640295:C:G	G549R	0.999
11:8640295:C:T	G549R	0.999
11:8640300:A:C	I547S	0.999

dbSNP variants (sampled 300 via entrez): RS1000019518 (11:8673725 T>A), RS1000020113 (11:8636165 CCA>C), RS1000036465 (11:8681402 G>A), RS1000090916 (11:8642665 A>G), RS1000152110 (11:8670414 T>C), RS1000159804 (11:8630134 G>A), RS1000167232 (11:8670764 T>C), RS1000180411 (11:8670701 C>T), RS1000207608 (11:8626399 A>G), RS1000219760 (11:8636509 C>T), RS1000259563 (11:8676233 T>C), RS1000294143 (11:8642321 A>G), RS1000418307 (11:8642983 G>A), RS1000432634 (11:8671007 G>A), RS1000459990 (11:8676263 T>C)

Disease associations

OMIM: gene MIM:612000 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

32 associations (top):

Study	Trait	p-value
GCST000880_26	Menarche (age at onset)	1.000000e-08
GCST002541_83	Menarche (age at onset)	3.000000e-12
GCST002783_113	Body mass index	2.000000e-08
GCST002783_128	Body mass index	3.000000e-11
GCST002783_359	Body mass index	8.000000e-12
GCST002783_597	Body mass index	9.000000e-06
GCST003993_17	Menarche (age at onset)	6.000000e-06
GCST004064_64	Waist-hip ratio	2.000000e-06
GCST004064_68	Waist-hip ratio	8.000000e-09
GCST004065_19	Waist circumference	5.000000e-09
GCST004065_7	Waist circumference	5.000000e-07
GCST004495_83	BMI (adjusted for smoking behaviour)	1.000000e-06
GCST004497_61	Body mass index (joint analysis main effects and smoking interaction)	5.000000e-06
GCST004557_17	Body mass index	2.000000e-10
GCST004557_200	Body mass index	2.000000e-09
GCST004557_229	Body mass index	9.000000e-11
GCST004557_99	Body mass index	1.000000e-09
GCST004558_14	Body mass index (joint analysis main effects and physical activity interaction)	1.000000e-09
GCST004558_149	Body mass index (joint analysis main effects and physical activity interaction)	2.000000e-10
GCST004558_171	Body mass index (joint analysis main effects and physical activity interaction)	8.000000e-09
GCST004558_97	Body mass index (joint analysis main effects and physical activity interaction)	1.000000e-08
GCST004559_12	Body mass index in physically active individuals	2.000000e-08
GCST004559_130	Body mass index in physically active individuals	1.000000e-07
GCST004559_50	Body mass index in physically active individuals	1.000000e-07
GCST004559_92	Body mass index in physically active individuals	1.000000e-08
GCST004904_234	Body mass index	1.000000e-17
GCST005950_10	Body mass index x sex x age interaction (4df test)	6.000000e-11
GCST005951_51	Body mass index	8.000000e-11
GCST005953_4	Body mass index (age <50)	4.000000e-11
GCST006802_3	Body mass index	2.000000e-06

EFO canonical traits (8, from GWAS)

EFO ID	Trait name
EFO:0004703	age at menarche
EFO:0004340	body mass index
EFO:0004343	waist-hip ratio
EFO:0004318	smoking behavior
EFO:0008002	physical activity measurement
EFO:0008007	age at assessment
EFO:0008343	sex interaction measurement
EFO:0008111	diet measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4296267 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: other protein — Non-enzymatic BRD containing proteins

CTD chemical–gene interactions

26 total (human), top 26 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	affects methylation	2
Tobacco Smoke Pollution	decreases expression	2
Cadmium Chloride	decreases expression, increases expression	2
aristolochic acid I	increases expression	1
GSK-J4	decreases expression	1
bisphenol F	affects cotreatment, decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
benzo(e)pyrene	decreases methylation	1
abrine	decreases expression	1
bisphenol S	affects cotreatment, decreases expression	1
jinfukang	increases expression	1
(+)-JQ1 compound	increases expression	1
Leflunomide	decreases expression	1
Cisplatin	increases expression	1
Dexamethasone	affects cotreatment, decreases expression	1
Indomethacin	affects cotreatment, decreases expression	1
Methapyrilene	decreases methylation	1
Urethane	decreases expression	1
Valproic Acid	increases methylation	1
1-Methyl-3-isobutylxanthine	affects cotreatment, decreases expression	1
1-Methyl-4-phenylpyridinium	increases expression	1
Cyclosporine	decreases expression	1
Aflatoxin B1	decreases methylation	1
Okadaic Acid	decreases expression	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4650193	Binding	Thermal Shift Assay. Domain start/stop: M1091-S1218	EUbOPEN bromodomain assay panel

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_TU49	HAP1 TRIM66 (-) 1	Cancer cell line	Male
CVCL_TU50	HAP1 TRIM66 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.