TRIM74

Summary

TRIM74 (tripartite motif containing 74, HGNC:17453) is a protein-coding gene on chromosome 7q11.23, encoding Tripartite motif-containing protein 74 (Q86UV6).

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in innate immune response. Located in cytosol.

Source: NCBI Gene 378108 — RefSeq curated summary.

At a glance

• MANE Select transcript: NM_198853

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:17453
Approved symbol	TRIM74
Name	tripartite motif containing 74
Location	7q11.23
Locus type	gene with protein product
Status	Approved
Aliases	MGC45440
Ensembl gene	ENSG00000155428
Ensembl biotype	protein_coding
OMIM	612550
Entrez	378108

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 3 protein_coding

ENST00000285805, ENST00000395244, ENST00000967371

RefSeq mRNA: 2 — MANE Select: NM_198853 NM_001317815, NM_198853

CCDS: CCDS5545

Canonical transcript exons

ENST00000285805 — 5 exons

Exon	Start	End
ENSE00002439680	72965759	72966175
ENSE00002451819	72961350	72961445
ENSE00002482322	72959485	72959908
ENSE00002483795	72969285	72969466
ENSE00002528519	72960021	72960251

Expression profiles

Bgee: expression breadth ubiquitous, 133 present calls, max score 75.16.

Top tissues by expression

136 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
body of stomach	UBERON:0001161	75.16	gold quality
stomach	UBERON:0000945	71.77	gold quality
mucosa of stomach	UBERON:0001199	71.15	gold quality
cerebellar hemisphere	UBERON:0002245	70.28	gold quality
right testis	UBERON:0004534	70.16	gold quality
right hemisphere of cerebellum	UBERON:0014890	70.13	gold quality
cerebellar cortex	UBERON:0002129	69.99	gold quality
cerebellum	UBERON:0002037	69.92	gold quality
right uterine tube	UBERON:0001302	69.47	gold quality
left testis	UBERON:0004533	68.79	gold quality
fundus of stomach	UBERON:0001160	68.33	gold quality
testis	UBERON:0000473	68.25	gold quality
sural nerve	UBERON:0015488	67.91	gold quality
granulocyte	CL:0000094	67.68	gold quality
right adrenal gland cortex	UBERON:0035827	66.70	gold quality
right lobe of liver	UBERON:0001114	66.69	gold quality
right adrenal gland	UBERON:0001233	64.88	gold quality
left adrenal gland	UBERON:0001234	64.81	gold quality
apex of heart	UBERON:0002098	64.66	gold quality
left adrenal gland cortex	UBERON:0035825	64.42	gold quality
left ovary	UBERON:0002119	63.67	gold quality
adrenal gland	UBERON:0002369	62.90	gold quality
body of uterus	UBERON:0009853	62.69	gold quality
left uterine tube	UBERON:0001303	62.65	gold quality
mucosa of transverse colon	UBERON:0004991	62.48	gold quality
tibial nerve	UBERON:0001323	61.91	gold quality
fallopian tube	UBERON:0003889	61.88	gold quality
pituitary gland	UBERON:0000007	61.25	gold quality
adenohypophysis	UBERON:0002196	61.17	gold quality
subcutaneous adipose tissue	UBERON:0002190	61.16	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.90

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

10 targeting TRIM74, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-3059-5P	99.70	69.93	2491
HSA-MIR-6832-5P	99.58	64.82	1132
HSA-MIR-766-5P	99.47	67.91	2225
HSA-MIR-6808-5P	99.31	66.23	2150
HSA-MIR-484	98.16	66.92	1074
HSA-MIR-6511B-5P	97.98	65.64	823
HSA-MIR-6811-5P	97.98	64.96	848
HSA-MIR-3137	97.26	66.78	761
HSA-MIR-6858-3P	96.37	64.41	771

Literature-anchored findings (GeneRIF, showing 1)

• Maps within the critical region of Williams-Beuren syndrome. (PMID:18398435)

Cross-species orthologs

0 orthologs

Paralogs (80): MID2 (ENSG00000080561), TRIM9 (ENSG00000100505), MID1 (ENSG00000101871), MEFV (ENSG00000103313), TRIM35 (ENSG00000104228), TRIM14 (ENSG00000106785), TRIM37 (ENSG00000108395), TRIM2 (ENSG00000109654), TRIM3 (ENSG00000110171), TRIM38 (ENSG00000112343), TRIM62 (ENSG00000116525), TRIM67 (ENSG00000119283), TRIM32 (ENSG00000119401), BSPRY (ENSG00000119411), TRIM25 (ENSG00000121060), TRIM6 (ENSG00000121236), TRIM24 (ENSG00000122779), TRIM51 (ENSG00000124900), TRIM28 (ENSG00000130726), TRIM21 (ENSG00000132109), TRIM5 (ENSG00000132256), TRIM22 (ENSG00000132274), TRIM47 (ENSG00000132481), TRIM45 (ENSG00000134253), TRIM29 (ENSG00000137699), TRIM54 (ENSG00000138100), PML (ENSG00000140464), TRIM65 (ENSG00000141569), TRIM43B (ENSG00000144010), TRIM43 (ENSG00000144015), TRIM7 (ENSG00000146054), TRIM41 (ENSG00000146063), TRIM50 (ENSG00000146755), TRIM4 (ENSG00000146833), TRIM55 (ENSG00000147573), TRIM48 (ENSG00000150244), TRIM36 (ENSG00000152503), TRIM11 (ENSG00000154370), TRIM42 (ENSG00000155890), TRIM63 (ENSG00000158022)

Protein

Protein identifiers

Tripartite motif-containing protein 74 — Q86UV6 (reviewed: Q86UV6)

Alternative names: Tripartite motif-containing protein 50C

All UniProt accessions (1): Q86UV6

UniProt curated annotations — full annotation on UniProt →

Similarity. Belongs to the TRIM/RBCC family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q86UV6-1	1	yes
Q86UV6-2	2

RefSeq proteins (2): NP_001304744, NP_942150* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000315	Znf_B-box	Domain
IPR001841	Znf_RING	Domain
IPR013083	Znf_RING/FYVE/PHD	Homologous_superfamily
IPR017907	Znf_RING_CS	Conserved_site
IPR027370	Znf-RING_euk	Domain
IPR050143	TRIM/RBCC	Family

Pfam: PF00643, PF13445

UniProt features (13 total): binding site 4, zinc finger region 2, sequence conflict 2, coiled-coil region 2, chain 1, sequence variant 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q86UV6-F1	88.40	0.54

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 89; 92; 111; 117

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 13 (showing top): GOBP_DEFENSE_RESPONSE_TO_OTHER_ORGANISM, chr7q11, GOMF_ACYLTRANSFERASE_ACTIVITY, GOMF_AMINOACYLTRANSFERASE_ACTIVITY, GOMF_UBIQUITIN_LIKE_PROTEIN_LIGASE_ACTIVITY, ZNF7_TARGET_GENES, MIR6811_5P, MIR6511B_5P, MIR3137, DESCARTES_MAIN_FETAL_PHOTORECEPTOR_CELLS, HOWARD_PBMC_INACT_MONOV_INFLUENZA_A_INDONESIA_05_2005_H5N1_AGE_19_39YO_AS03_ADJUVANT_VS_BUFFER_1DY_DN, HOEK_T_CELL_2011_2012_TIV_ADULT_1DY_DN, GOBP_BIOLOGICAL_PROCESS_INVOLVED_IN_INTERSPECIES_INTERACTION_BETWEEN_ORGANISMS

GO Biological Process (1): innate immune response (GO:0045087)

GO Molecular Function (4): zinc ion binding (GO:0008270), ubiquitin protein ligase activity (GO:0061630), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (2): cytoplasm (GO:0005737), cytosol (GO:0005829)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
immune response	1
defense response to symbiont	1
transition metal ion binding	1
ubiquitin-protein transferase activity	1
ubiquitin-like protein ligase activity	1
binding	1
cation binding	1
intracellular anatomical structure	1
cytoplasm	1

Protein interactions and networks

STRING

316 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TRIM74	BBOX1	O75936	775
TRIM74	TRIM56	Q9BRZ2	528
TRIM74	TRIM52	Q96A61	454
TRIM74	TRIM40	Q6P9F5	435
TRIM74	TMEM270	Q6UE05	433
TRIM74	RNF113B	Q8IZP6	432
TRIM74	NSUN5	Q96P11	418
TRIM74	KRTAP17-1	Q9BYP8	413
TRIM74	TRIM44	Q96DX7	411
TRIM74	TRIM23	P36406	410
TRIM74	FAM181A	Q8N9Y4	398
TRIM74	RNF207	Q6ZRF8	388
TRIM74	TRIM3	O75382	377
TRIM74	TRIML1	Q8N9V2	376
TRIM74	RFPL4A	A6NLU0	370
TRIM74	GTF2IRD2	Q86UP8	370

IntAct

12 interactions, top by confidence:

A	B	Type	Score
TRIM74	DTNBP1	psi-mi:“MI:0915”(physical association)	0.400
HSP90AB1	TRIM74	psi-mi:“MI:0915”(physical association)	0.400
TRIM74	TSG101	psi-mi:“MI:0915”(physical association)	0.370
UBE2U	TRIM74	psi-mi:“MI:0915”(physical association)	0.370
TRIM74	RBX1	psi-mi:“MI:0915”(physical association)	0.370
TRIM74	MGRN1	psi-mi:“MI:0915”(physical association)	0.370
TRIM74	LRSAM1	psi-mi:“MI:0915”(physical association)	0.370
TRIM74	BRCA1	psi-mi:“MI:0915”(physical association)	0.370
RNF32	TRIM74	psi-mi:“MI:0915”(physical association)	0.370
ECE1	TRIM74	psi-mi:“MI:0915”(physical association)	0.370
TRIM74	FECH	psi-mi:“MI:0914”(association)	0.350

BioGRID (21): TRIM74 (Two-hybrid), CNOT1 (Affinity Capture-MS), FECH (Affinity Capture-MS), DTNBP1 (Affinity Capture-MS), DTNBP1 (Affinity Capture-MS), DTNBP1 (Affinity Capture-MS), FECH (Affinity Capture-MS), RQCD1 (Affinity Capture-MS), RFWD3 (Affinity Capture-MS), TRIM74 (Affinity Capture-MS), TRIM74 (Two-hybrid), TRIM74 (Two-hybrid), TRIM74 (Two-hybrid), TRIM74 (Two-hybrid), TRIM74 (Two-hybrid)

ESM2 similar proteins: A0JPQ4, E1BD59, O15197, P0C0K6, P62603, Q14142, Q1XH17, Q1XH18, Q3UWZ0, Q5BK82, Q5JZY3, Q5M929, Q5NCC3, Q5RBG2, Q5RKG6, Q5TM55, Q5W0U4, Q640S6, Q6P6S3, Q6PGR9, Q6PJ69, Q6ZMU5, Q7TPM3, Q7YR32, Q80VI1, Q80X56, Q80YW5, Q810I1, Q810I2, Q865W2, Q86UV6, Q86UV7, Q86XT4, Q8BFW4, Q8BVW3, Q8BYG9, Q8C006, Q8C0E3, Q8IUD6, Q8K243

Diamond homologs: A0A3B3IT33, A0JN74, A4QPC6, A6NCK2, A6NDI0, A6NGJ6, A6NI03, A6NLI5, A6NLU0, B1H278, B6VQ60, C9J1S8, D4ABM4, F6ZQ54, F8VTS6, I1YAP6, O00478, O00481, O15344, O70583, O75677, O75678, O75679, O76064, P0CI25, P0CI26, P18892, P19474, P82458, P86448, P86449, Q02398, Q03605, Q13410, Q14258, Q1L5Z9, Q1XHT8, Q2HJ46, Q2T9Z0, Q3TL54

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
Ub:E2	“up-regulates activity”	TRIM74	ubiquitination

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

890 predictions. Top by Δscore:

Variant	Effect	Δscore
7:72961348:A:AC	donor_gain	1.0000
7:72961349:C:CC	donor_gain	1.0000
7:72961349:CG:C	donor_gain	1.0000
7:72961349:CGA:C	donor_gain	1.0000
7:72961349:CGACG:C	donor_gain	1.0000
7:72959906:CTT:C	acceptor_gain	0.9900
7:72960016:CTCA:C	donor_loss	0.9900
7:72960017:TCA:T	donor_loss	0.9900
7:72960018:CACCC:C	donor_loss	0.9900
7:72960019:A:C	donor_loss	0.9900
7:72960019:AC:A	donor_gain	0.9900
7:72960020:CC:C	donor_gain	0.9900
7:72961347:CACG:C	donor_gain	0.9900
7:72961348:ACGA:A	donor_gain	0.9900
7:72961349:CGAC:C	donor_gain	0.9900
7:72960013:CCACT:C	donor_loss	0.9800
7:72960014:CACTC:C	donor_loss	0.9800
7:72960015:ACTCA:A	donor_loss	0.9800
7:72960019:A:AC	donor_gain	0.9800
7:72960020:C:CC	donor_gain	0.9800
7:72960248:CATT:C	acceptor_gain	0.9800
7:72960252:C:CC	acceptor_gain	0.9800
7:72961343:CACT:C	donor_loss	0.9800
7:72961345:C:G	donor_loss	0.9800
7:72961347:C:CC	donor_loss	0.9800
7:72961348:A:C	donor_loss	0.9800
7:72961441:TCCTC:T	acceptor_gain	0.9800
7:72961442:CCTCC:C	acceptor_gain	0.9800
7:72961443:CTC:C	acceptor_gain	0.9800
7:72961446:C:CC	acceptor_gain	0.9800

AlphaMissense

1642 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:72965861:G:C	F99L	0.968
7:72965861:G:T	F99L	0.968
7:72965863:A:G	F99L	0.968
7:72960210:G:C	F179L	0.949
7:72960210:G:T	F179L	0.949
7:72960212:A:G	F179L	0.949
7:72960027:G:C	F240L	0.941
7:72960027:G:T	F240L	0.941
7:72960029:A:G	F240L	0.941
7:72959903:G:C	F244L	0.904
7:72959903:G:T	F244L	0.904
7:72959905:A:G	F244L	0.904
7:72961363:C:G	R161P	0.881
7:72966089:G:C	F23L	0.878
7:72966089:G:T	F23L	0.878
7:72966091:A:G	F23L	0.878
7:72960234:G:C	F171L	0.874
7:72960234:G:T	F171L	0.874
7:72960236:A:G	F171L	0.874
7:72961357:C:G	R163P	0.868
7:72965759:C:A	K133N	0.852
7:72965759:C:G	K133N	0.852
7:72965826:C:G	C111S	0.843
7:72965827:A:T	C111S	0.843
7:72965867:G:C	S97R	0.838
7:72965867:G:T	S97R	0.838
7:72965869:T:G	S97R	0.838
7:72965862:A:G	F99S	0.834
7:72965836:A:G	C108R	0.830
7:72965798:G:C	H120Q	0.809

dbSNP variants (sampled 300 via entrez): RS10240489 (7:72971507 C>T), RS10240494 (7:72971518 C>T), RS10240512 (7:72971557 C>T), RS10240526 (7:72971590 C>T), RS111681123 (7:72971573 G>A), RS112471455 (7:72969495 C>T), RS113635218 (7:72967702 C>A,T), RS1156562169 (7:72964013 G>A,T), RS1156637981 (7:72962398 C>A), RS1156705668 (7:72963249 T>A), RS1156814654 (7:72959541 G>A), RS1157027874 (7:72954716 A>C), RS1157034205 (7:72969788 C>G,T), RS1157205869 (7:72969172 T>C), RS1157321442 (7:72967436 T>C)

Disease associations

OMIM: gene MIM:612550 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

7 total (human), top 7 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
aristolochic acid I	increases expression	1
triphenyl phosphate	affects expression	1
licochalcone B	increases expression	1
Doxorubicin	affects expression	1
Tobacco Smoke Pollution	decreases expression	1
Valproic Acid	increases methylation	1
Okadaic Acid	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.