TSSK3

Summary

TSSK3 (testis specific serine kinase 3, HGNC:15473) is a protein-coding gene on chromosome 1p35.1, encoding Testis-specific serine/threonine-protein kinase 3 (Q96PN8). Serine/threonine protein kinase required for spermatid development and male fertility.

This gene encodes a kinase expressed exclusively in the testis that is thought to play a role in either germ cell differentiation or mature sperm function.

Source: NCBI Gene 81629 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 49 total
• Druggable target: yes — 1 molecules with ChEMBL bioactivity
• MANE Select transcript: NM_052841

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:15473
Approved symbol	TSSK3
Name	testis specific serine kinase 3
Location	1p35.1
Locus type	gene with protein product
Status	Approved
Aliases	SPOGA3
Ensembl gene	ENSG00000162526
Ensembl biotype	protein_coding
OMIM	607660
Entrez	81629

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 2 protein_coding, 2 protein_coding_CDS_not_defined

ENST00000373534, ENST00000421153, ENST00000574315, ENST00000574792

RefSeq mRNA: 1 — MANE Select: NM_052841 NM_052841

CCDS: CCDS362

Canonical transcript exons

ENST00000373534 — 2 exons

Exon	Start	End
ENSE00001460783	32363595	32364312
ENSE00001460785	32362539	32362846

Expression profiles

Bgee: expression breadth ubiquitous, 159 present calls, max score 94.69.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 2.6814 / max 113.6734, expressed in 1112 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
1984	1.3580	764
1985	1.2438	671
1987	0.0662	6
1986	0.0134	3

Top tissues by expression

245 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sperm	CL:0000019	94.69	gold quality
right testis	UBERON:0004534	92.39	gold quality
left testis	UBERON:0004533	92.15	gold quality
testis	UBERON:0000473	89.23	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	87.67	gold quality
right ovary	UBERON:0002118	79.68	gold quality
left ovary	UBERON:0002119	79.16	gold quality
sural nerve	UBERON:0015488	78.87	gold quality
stromal cell of endometrium	CL:0002255	78.52	gold quality
right uterine tube	UBERON:0001302	78.50	gold quality
vena cava	UBERON:0004087	78.36	gold quality
body of uterus	UBERON:0009853	78.27	gold quality
cerebellar hemisphere	UBERON:0002245	78.15	gold quality
cerebellar cortex	UBERON:0002129	78.08	gold quality
right hemisphere of cerebellum	UBERON:0014890	77.95	gold quality
mucosa of stomach	UBERON:0001199	77.83	gold quality
subthalamic nucleus	UBERON:0001906	77.58	gold quality
left uterine tube	UBERON:0001303	77.50	gold quality
endocervix	UBERON:0000458	77.43	gold quality
cerebellum	UBERON:0002037	77.34	gold quality
right lobe of thyroid gland	UBERON:0001119	77.32	gold quality
apex of heart	UBERON:0002098	76.60	gold quality
right lung	UBERON:0002167	76.54	gold quality
granulocyte	CL:0000094	76.47	gold quality
left lobe of thyroid gland	UBERON:0001120	76.44	gold quality
epithelium of nasopharynx	UBERON:0001951	76.07	gold quality
thyroid gland	UBERON:0002046	75.99	gold quality
left adrenal gland cortex	UBERON:0035825	75.75	gold quality
dorsal plus ventral thalamus	UBERON:0001897	75.74	gold quality
gastrocnemius	UBERON:0001388	75.65	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.07

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 3)

• human homolog of mouse stk22d; It was cloned and found it to be expressed exclusively in the testis. (PMID:11597141)
• PDK1-induced phosphorylation increased in vitro TSSK3 kinase activity, suggesting that TSSK3 can be regulated in the same way as AGC kinase family members. The peptide sequence RRSSSY containing Ser5 is identified as a target for TSSK3 phosphorylation (PMID:16336268)
• TSSK3, a novel target for male contraception, is required for spermiogenesis. (PMID:34623009)

Cross-species orthologs

9 orthologs

Organism	Symbol	Gene ID
danio_rerio	nim1kb	ENSDARG00000079031
mus_musculus	Tssk3	ENSMUSG00000000411
rattus_norvegicus	Tssk3	ENSRNOG00000008693
drosophila_melanogaster	Snrk	FBGN0033915
drosophila_melanogaster	Nuak	FBGN0262617
caenorhabditis_elegans		WBGENE00012638
caenorhabditis_elegans	ZK524.4	WBGENE00013994
caenorhabditis_elegans	tag-344	WBGENE00015230
caenorhabditis_elegans		WBGENE00044388

Paralogs (17): NUAK1 (ENSG00000074590), PRKAA1 (ENSG00000132356), TSSK4 (ENSG00000139908), HUNK (ENSG00000142149), SIK1 (ENSG00000142178), BRSK1 (ENSG00000160469), SIK3 (ENSG00000160584), PRKAA2 (ENSG00000162409), NUAK2 (ENSG00000163545), SNRK (ENSG00000163788), MELK (ENSG00000165304), SIK2 (ENSG00000170145), BRSK2 (ENSG00000174672), NIM1K (ENSG00000177453), TSSK6 (ENSG00000178093), TSSK2 (ENSG00000206203), TSSK1B (ENSG00000212122)

Protein

Protein identifiers

Testis-specific serine/threonine-protein kinase 3 — Q96PN8 (reviewed: Q96PN8)

Alternative names: Serine/threonine-protein kinase 22C

All UniProt accessions (2): Q96PN8, I3L1X4

UniProt curated annotations — full annotation on UniProt →

Function. Serine/threonine protein kinase required for spermatid development and male fertility.

Subcellular location. Cell projection. Cilium. Flagellum.

Post-translational modifications. Autophosphorylated at Ser-166. Phosphorylation at Thr-168 by PDPK1 activates the serine/threonine protein kinase activity.

Activity regulation. Activated by phosphorylation on Thr-168 by PDPK1.

Cofactor. Can use both Mg(2+) and Mn(2+) as cofactor, with a preference for Mn(2+) in vitro.

Similarity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

RefSeq proteins (1): NP_443073* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000719	Prot_kinase_dom	Domain
IPR011009	Kinase-like_dom_sf	Homologous_superfamily
IPR017441	Protein_kinase_ATP_BS	Binding_site
IPR042709	TSSK3_STKc	Domain

Pfam: PF00069

Catalyzed reactions (Rhea), 2 shown:

• L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (RHEA:17989)
• L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (RHEA:46608)

UniProt features (10 total): sequence variant 3, binding site 2, modified residue 2, chain 1, domain 1, active site 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96PN8-F1	93.65	0.83

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 134 (proton acceptor)

Ligand- & substrate-binding residues (2): 16–24; 39

Post-translational modifications (2): 166, 168

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 106 (showing top): AAGCAAT_MIR137, MAZ_Q6, TGACCTY_ERR1_Q2, GOBP_MALE_GAMETE_GENERATION, AAAYRNCTG_UNKNOWN, GATA1_01, GRE_C, GOBP_CELLULAR_PROCESS_INVOLVED_IN_REPRODUCTION_IN_MULTICELLULAR_ORGANISM, HIF1_Q3, GOBP_DEVELOPMENTAL_PROCESS_INVOLVED_IN_REPRODUCTION, CDPCR3HD_01, YY1_01, DBP_Q6, AP2_Q6_01, AP2GAMMA_01

GO Biological Process (4): protein phosphorylation (GO:0006468), spermatogenesis (GO:0007283), spermatid development (GO:0007286), cell differentiation (GO:0030154)

GO Molecular Function (11): magnesium ion binding (GO:0000287), protein serine/threonine kinase activity (GO:0004674), ATP binding (GO:0005524), manganese ion binding (GO:0030145), protein serine kinase activity (GO:0106310), nucleotide binding (GO:0000166), protein kinase activity (GO:0004672), protein binding (GO:0005515), kinase activity (GO:0016301), transferase activity (GO:0016740), metal ion binding (GO:0046872)

GO Cellular Component (4): sperm flagellum (GO:0036126), cilium (GO:0005929), motile cilium (GO:0031514), cell projection (GO:0042995)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein kinase activity	2
phosphorylation	1
protein modification process	1
developmental process involved in reproduction	1
male gamete generation	1
germ cell development	1
spermatid differentiation	1
cellular developmental process	1
metal ion binding	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
transition metal ion binding	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
kinase activity	1
phosphotransferase activity, alcohol group as acceptor	1
catalytic activity, acting on a protein	1
binding	1
transferase activity, transferring phosphorus-containing groups	1
catalytic activity	1
cation binding	1
9+2 motile cilium	1
intraciliary transport particle	1
membrane-bounded organelle	1
plasma membrane bounded cell projection	1
cilium	1
cellular anatomical structure	1

Protein interactions and networks

STRING

1581 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TSSK3	TSKS	Q9UJT2	720
TSSK3	CALY	Q9NYX4	594
TSSK3	KLHL10	Q6JEL2	514
TSSK3	CRABP1	P29762	454
TSSK3	SIGLEC1	Q9BZZ2	452
TSSK3	SSMEM1	Q8WWF3	417
TSSK3	TNP1	P09430	401
TSSK3	IZUMO1	Q8IYV9	397
TSSK3	FAM170B	A6NMN3	392
TSSK3	TXNDC2	Q86VQ3	383
TSSK3	SPATC1	Q76KD6	382
TSSK3	CCDC70	Q6NSX1	380
TSSK3	PRM2	P04554	364
TSSK3	SOX30	O94993	354
TSSK3	TCP11	Q8WWU5	351

IntAct

216 interactions, top by confidence:

A	B	Type	Score
TSSK3	LNX1	psi-mi:“MI:0915”(physical association)	0.780
LNX1	TSSK3	psi-mi:“MI:0915”(physical association)	0.780
GOLGA2	TSSK3	psi-mi:“MI:0915”(physical association)	0.720
EFHC2	TSSK3	psi-mi:“MI:0915”(physical association)	0.720
TSSK3	VPS52	psi-mi:“MI:0915”(physical association)	0.720
TSSK3	RBCK1	psi-mi:“MI:0915”(physical association)	0.720
IKZF3	TSSK3	psi-mi:“MI:0915”(physical association)	0.720
TSSK3	GOLGA2	psi-mi:“MI:0915”(physical association)	0.720
TSSK3	EFHC2	psi-mi:“MI:0915”(physical association)	0.720
VPS52	TSSK3	psi-mi:“MI:0915”(physical association)	0.720
TSSK3	IKZF3	psi-mi:“MI:0915”(physical association)	0.720
DDIT4L	TSSK3	psi-mi:“MI:0915”(physical association)	0.600
TCF4	TSSK3	psi-mi:“MI:0915”(physical association)	0.560
TSSK3	ZBTB25	psi-mi:“MI:0915”(physical association)	0.560
TSSK3	REL	psi-mi:“MI:0915”(physical association)	0.560
TLE5	TSSK3	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (88): TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), LNX1 (Two-hybrid), TRIM9 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Two-hybrid), TSSK3 (Biochemical Activity)

ESM2 similar proteins: A0A5B9GBF0, A1IVT7, A3B529, B2MVY4, O65554, O70150, O70444, P05128, P11802, P23111, P27638, P29618, P30285, P34112, P35426, P48609, P52389, P58750, P79432, P93101, Q05006, Q0U4L8, Q15759, Q1DUU8, Q2PQN9, Q2U469, Q32KY4, Q38772, Q38HL5, Q41639, Q5JLD8, Q683C9, Q69Q47, Q6ERS4, Q6P2M8, Q6SA08, Q80YP0, Q86V86, Q8C1R0, Q8TGA9

Diamond homologs: A2XFF4, A3B529, A8WYE4, B8BBT7, O08678, O08679, O22932, O22971, O54863, O60285, O65554, O94168, P06782, P27448, P54645, P57059, P92937, P92958, Q00372, Q02723, Q03141, Q05512, Q0D4B2, Q0JI49, Q10LQ2, Q12263, Q13131, Q21017, Q28GW8, Q2QY53, Q2QYM3, Q2RAX3, Q2RBF0, Q38997, Q3SZW1, Q54DF2, Q54TA3, Q54YF2, Q5EG47, Q5JLD8

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
TSSK3	“up-regulates activity”	TSSK3	phosphorylation
PDPK1	“up-regulates activity”	TSSK3	phosphorylation

Disease & clinical

Clinical variants and AI predictions

ClinVar

49 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	46
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

435 predictions. Top by Δscore:

Variant	Effect	Δscore
1:32362417:GGC:G	donor_gain	1.0000
1:32361725:CTTA:C	donor_loss	0.9900
1:32361726:TTACC:T	donor_loss	0.9900
1:32361727:TACC:T	donor_loss	0.9900
1:32361728:A:T	donor_loss	0.9900
1:32361729:C:CA	donor_loss	0.9900
1:32362418:GC:G	donor_gain	0.9900
1:32362419:C:G	donor_gain	0.9900
1:32363585:A:AG	acceptor_gain	0.9900
1:32363591:TCAG:T	acceptor_gain	0.9900
1:32363593:A:AC	acceptor_loss	0.9900
1:32363593:A:AG	acceptor_gain	0.9900
1:32363594:G:GG	acceptor_gain	0.9900
1:32363594:G:T	acceptor_loss	0.9900
1:32363594:GA:G	acceptor_gain	0.9900
1:32363594:GAGT:G	acceptor_gain	0.9900
1:32361729:CCTGA:C	donor_gain	0.9800
1:32361731:TG:T	donor_gain	0.9800
1:32362416:GGGC:G	donor_gain	0.9800
1:32362430:G:GA	donor_gain	0.9800
1:32362433:G:GA	donor_gain	0.9800
1:32362435:T:TA	donor_gain	0.9800
1:32362436:A:AA	donor_gain	0.9800
1:32363591:TCAGA:T	acceptor_gain	0.9800
1:32363592:CAG:C	acceptor_gain	0.9800
1:32363592:CAGAG:C	acceptor_gain	0.9800
1:32363593:AGA:A	acceptor_gain	0.9800
1:32363594:GAG:G	acceptor_gain	0.9800
1:32363594:GAGTT:G	acceptor_gain	0.9800
1:32361728:A:AC	donor_gain	0.9700

AlphaMissense

1772 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:32362818:A:C	K39N	0.999
1:32362818:A:T	K39N	0.999
1:32363847:G:C	R133P	0.998
1:32363850:A:C	D134A	0.998
1:32363850:A:T	D134V	0.998
1:32363901:A:T	D151V	0.998
1:32363902:C:A	D151E	0.998
1:32363902:C:G	D151E	0.998
1:32364077:T:C	F210L	0.998
1:32364079:T:A	F210L	0.998
1:32364079:T:G	F210L	0.998
1:32363850:A:G	D134G	0.997
1:32363851:C:A	D134E	0.997
1:32363851:C:G	D134E	0.997
1:32363857:A:C	K136N	0.997
1:32363857:A:T	K136N	0.997
1:32364029:T:A	W194R	0.997
1:32364029:T:C	W194R	0.997
1:32363609:T:C	F54L	0.996
1:32363611:C:A	F54L	0.996
1:32363611:C:G	F54L	0.996
1:32363855:A:G	K136E	0.996
1:32363856:A:T	K136I	0.996
1:32363901:A:C	D151A	0.996
1:32363963:A:C	S172R	0.996
1:32363965:T:A	S172R	0.996
1:32363965:T:G	S172R	0.996
1:32362811:C:A	A37E	0.995
1:32362816:A:G	K39E	0.995
1:32363913:C:A	A155D	0.995

dbSNP variants (sampled 300 via entrez): RS1000068694 (1:32362322 A>G), RS1000652777 (1:32361200 G>A), RS1000982482 (1:32360986 C>T), RS1001722336 (1:32360696 G>A), RS1002459355 (1:32361849 G>A), RS1002472014 (1:32360794 A>G), RS1003416754 (1:32364449 CCTT>C), RS1003728868 (1:32363037 C>A,G,T), RS1004007596 (1:32364338 T>C), RS1004249861 (1:32364560 C>T), RS1004800162 (1:32361860 C>T), RS1004872163 (1:32362080 G>A,C), RS1005879120 (1:32363011 G>A,T), RS1006441808 (1:32361803 A>C), RS1006569479 (1:32362698 C>A,T)

Disease associations

OMIM: gene MIM:607660 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST008758_22	Pre-treatment viral load in HIV-1 infection	4.000000e-16

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0010125	viral load

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL3414416 (SINGLE PROTEIN)

Molecules with ChEMBL bioactivity

1 molecules (phase ≥1), by development phase (incl. off-target/promiscuous compounds). Patent mentions across the top 20 by phase: 1,538 (via chembl_molecule»patent_compound — counts attach to the compound, not the gene–compound relationship, so off-target/promiscuous molecules can dominate).

Molecule	Name	Phase	Patents
CHEMBL1232461	MOLIBRESIB	2	1,538

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: enzyme — Testis specific kinase (TSSK) family

ChEMBL bioactivities

10 potent at pChembl≥5 of 10 total, top 10 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.49	IC50	3.21	nM	STAUROSPORINE
8.15	IC50	7.01	nM	STAUROSPORINE
7.83	IC50	14.9	nM	STAUROSPORINE
7.04	IC50	91	nM	CHEMBL2334586
6.48	Kd	330	nM	CHEMBL4576489
6.42	Kd	380	nM	CHEMBL4465866
6.38	IC50	420	nM	CHEMBL3415608
6.38	IC50	420	nM	CHEMBL3943181
6.00	IC50	1000	nM	TP-030n
5.00	IC50	1e+04	nM	MOLIBRESIB

PubChem BioAssay actives

9 with measured affinity, of 67 total; 7 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
(2S,3R,4R,6R)-3-methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one	1715136: Inhibition of human TSSK3 using KKKVSRSGLYRSPSMPENLNRPR as substrate by [gamma-33P]-ATP assay	ic50	0.0032	uM
4-(4-amino-3,5,12-triazatetracyclo[9.7.0.02,7.013,18]octadeca-1(11),2,4,6,13(18),14,16-heptaen-16-yl)-2-methylbut-3-yn-2-ol	1856574: Inhibition of wild-type human full length TSSK3 (M1 to T268 residues) expressed in mammalian expression system by Kinomescan method	ic50	0.0910	uM
3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)-N-[2-[2-[2-[2-[[(2S,3R,4R,6R)-3-methoxy-2-methyl-16-oxo-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-4-yl]-methylamino]ethoxy]ethoxy]ethoxy]ethyl]propanamide	1526261: Binding affinity to recombinant full-length N-terminal His-FLAG-GST-tagged TSSK3 (unknown origin) expressed in baculovirus infected Sf9 insect cells incubated for 1 hr by TR-FRET assay	kd	0.3300	uM
3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)-N-[2-[2-[2-[2-[[(2S,3R,4R,6R)-3-methoxy-2-methyl-16-oxo-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-4-yl]amino]ethoxy]ethoxy]ethoxy]ethyl]propanamide	1526261: Binding affinity to recombinant full-length N-terminal His-FLAG-GST-tagged TSSK3 (unknown origin) expressed in baculovirus infected Sf9 insect cells incubated for 1 hr by TR-FRET assay	kd	0.3800	uM
5-[[(1R,2S)-2-aminocyclohexyl]amino]-3-(1H-benzimidazol-2-yl)pyridine-2-carboxamide	1199576: Inhibition of TSSK3 (unknown origin)	ic50	0.4200	uM
5-[[(1R,2S)-2-aminocyclohexyl]amino]-3-(1H-benzimidazol-2-yl)pyrazine-2-carboxamide	1326206: Inhibition of TSSK3 (unknown origin)	ic50	0.4200	uM
2-[(4S)-6-(4-chlorophenyl)-8-methoxy-1-methyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepin-4-yl]-N-ethylacetamide	2178641: Inhibition of TSSK3 (unknown origin) incubated for 1 hr by colloidal coomassie staining based LC-MS/MS analysis	ic50	10.0000	uM

CTD chemical–gene interactions

11 total (human), top 11 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
aminomethylphosphonic acid (AMPA)	decreases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
jinfukang	increases expression, affects cotreatment	1
Air Pollutants	increases expression, increases abundance	1
Cisplatin	affects cotreatment, increases expression	1
Estradiol	decreases expression	1
Folic Acid	increases expression	1
Valproic Acid	increases methylation	1
2,4-Dichlorophenoxyacetic Acid	decreases expression	1
Okadaic Acid	decreases expression	1
Particulate Matter	increases abundance, increases expression	1

ChEMBL screening assays

83 unique, capped per target: 83 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL3418524	Binding	Inhibition of TSSK3 (unknown origin)	Overcoming mutagenicity and ion channel activity: optimization of selective spleen tyrosine kinase inhibitors. — J Med Chem

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2JV	Abcam HeLa TSSK3 KO	Cancer cell line	Female
CVCL_TV00	HAP1 TSSK3 (-)	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.