TSSK6

Summary

TSSK6 (testis specific serine kinase 6, HGNC:30410) is a protein-coding gene on chromosome 19p13.11, encoding Testis-specific serine/threonine-protein kinase 6 (Q9BXA6). Serine/threonine-protein kinase component of the sperm flagellar doublet microtubules.

This intronless gene encodes a member of the CAMK (calcium/calmodulin-dependent) serine/threonine protein kinase family. The encoded kinase has a broad expression pattern but is described as testis-specific due to effects on fertility. Male mice which lack the gene encoding a highly similar protein are sterile and have morphologically abnormal sperm.

Source: NCBI Gene 83983 — RefSeq curated summary.

At a glance

• GWAS associations: 13
• Clinical variants (ClinVar): 22 total
• Druggable target: yes
• MANE Select transcript: NM_032037

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30410
Approved symbol	TSSK6
Name	testis specific serine kinase 6
Location	19p13.11
Locus type	gene with protein product
Status	Approved
Aliases	SSTK, FLJ24002, CT72
Ensembl gene	ENSG00000178093
Ensembl biotype	protein_coding
OMIM	610712
Entrez	83983

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 2 nonsense_mediated_decay, 1 protein_coding

ENST00000585580, ENST00000587522, ENST00000602623

RefSeq mRNA: 1 — MANE Select: NM_032037 NM_032037

CCDS: CCDS12403

Canonical transcript exons

ENST00000585580 — 1 exons

Exon	Start	End
ENSE00002812060	19514219	19515548

Expression profiles

Bgee: expression breadth ubiquitous, 157 present calls, max score 97.83.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.1052 / max 101.7892, expressed in 4 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
180147	0.1052	4

Top tissues by expression

252 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	97.83	gold quality
right testis	UBERON:0004534	97.71	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	95.77	gold quality
sperm	CL:0000019	95.33	gold quality
testis	UBERON:0000473	94.10	gold quality
pancreatic ductal cell	CL:0002079	86.33	silver quality
adult organism	UBERON:0007023	74.81	gold quality
buccal mucosa cell	CL:0002336	74.79	silver quality
granulocyte	CL:0000094	74.78	gold quality
stromal cell of endometrium	CL:0002255	70.12	gold quality
parotid gland	UBERON:0001831	70.08	gold quality
mucosa of transverse colon	UBERON:0004991	69.40	gold quality
tendon of biceps brachii	UBERON:0008188	69.34	gold quality
skin of leg	UBERON:0001511	67.09	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	67.09	gold quality
right lobe of liver	UBERON:0001114	66.42	gold quality
metanephros cortex	UBERON:0010533	65.51	gold quality
right uterine tube	UBERON:0001302	65.13	gold quality
body of stomach	UBERON:0001161	64.69	gold quality
blood	UBERON:0000178	64.58	gold quality
spleen	UBERON:0002106	64.45	gold quality
right adrenal gland	UBERON:0001233	64.28	gold quality
right adrenal gland cortex	UBERON:0035827	64.26	gold quality
left adrenal gland	UBERON:0001234	64.17	gold quality
skin of abdomen	UBERON:0001416	64.04	gold quality
body of pancreas	UBERON:0001150	63.94	gold quality
zone of skin	UBERON:0000014	63.88	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	63.85	gold quality
left adrenal gland cortex	UBERON:0035825	63.68	gold quality
ventricular zone	UBERON:0003053	63.51	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-GEOD-134144	yes	32.67
E-ANND-3	no	0.72

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): NR1I2

miRNA regulators (miRDB)

24 targeting TSSK6, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5196-5P	100.00	67.98	2761
HSA-MIR-4747-5P	100.00	67.90	2681
HSA-MIR-32-5P	99.98	75.21	1964
HSA-MIR-92A-3P	99.98	75.21	1960
HSA-MIR-92B-3P	99.98	75.25	1955
HSA-MIR-25-3P	99.98	74.60	1817
HSA-MIR-363-3P	99.98	74.72	1821
HSA-MIR-367-3P	99.98	74.83	1819
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-137-3P	99.87	74.74	2401
HSA-MIR-4728-5P	99.85	69.39	4718
HSA-MIR-6785-5P	99.82	68.68	4428
HSA-MIR-149-3P	99.72	68.22	3963
HSA-MIR-6883-5P	99.69	68.05	3785
HSA-MIR-657	99.48	66.02	848
HSA-MIR-92A-1-5P	98.28	64.51	631
HSA-MIR-6842-3P	98.07	66.33	1325
HSA-MIR-647	97.73	67.79	927
HSA-MIR-518A-3P	93.85	64.90	108
HSA-MIR-518B	93.85	64.62	111
HSA-MIR-518C-3P	93.85	64.62	111
HSA-MIR-518D-3P	93.85	64.59	111
HSA-MIR-518F-3P	93.85	64.93	108
HSA-MIR-526A-3P	93.85	65.32	108

Literature-anchored findings (GeneRIF, showing 3)

• results demonstrate that SSTK is required for proper postmeiotic chromatin remodeling and male fertility (PMID:15870294)
• findings, for the first time, suggested an association of c.822+126T>G/C in TSSK6 with spermatogenic impairment in humans in which allele T may be a risk factor for male infertility, while alleles C and G may decrease susceptibility to male infertility (PMID:20037600)
• Testis-specific serine kinase 6 (TSSK6) is abnormally expressed in colorectal cancer and promotes oncogenic behaviors. (PMID:38762178)

Cross-species orthologs

9 orthologs

Organism	Symbol	Gene ID
danio_rerio	tssk6	ENSDARG00000017994
mus_musculus	Tssk6	ENSMUSG00000047654
rattus_norvegicus	Tssk6	ENSRNOG00000020599
drosophila_melanogaster	Snrk	FBGN0033915
drosophila_melanogaster	Nuak	FBGN0262617
caenorhabditis_elegans		WBGENE00012638
caenorhabditis_elegans	ZK524.4	WBGENE00013994
caenorhabditis_elegans	tag-344	WBGENE00015230
caenorhabditis_elegans		WBGENE00044388

Paralogs (17): NUAK1 (ENSG00000074590), PRKAA1 (ENSG00000132356), TSSK4 (ENSG00000139908), HUNK (ENSG00000142149), SIK1 (ENSG00000142178), BRSK1 (ENSG00000160469), SIK3 (ENSG00000160584), PRKAA2 (ENSG00000162409), TSSK3 (ENSG00000162526), NUAK2 (ENSG00000163545), SNRK (ENSG00000163788), MELK (ENSG00000165304), SIK2 (ENSG00000170145), BRSK2 (ENSG00000174672), NIM1K (ENSG00000177453), TSSK2 (ENSG00000206203), TSSK1B (ENSG00000212122)

Protein

Protein identifiers

Testis-specific serine/threonine-protein kinase 6 — Q9BXA6 (reviewed: Q9BXA6)

Alternative names: Cancer/testis antigen 72, Serine/threonine-protein kinase SSTK, Small serine/threonine kinase

All UniProt accessions (3): A0A024R7Q5, Q9BXA6, R4GMZ2

UniProt curated annotations — full annotation on UniProt →

Function. Serine/threonine-protein kinase component of the sperm flagellar doublet microtubules. May act as a regulator of sperm motility by mediating phosphorylation of sperm doublet microtubule proteins. Plays a role in DNA condensation during postmeiotic chromatin remodeling and histone-to-protamine transition during spermatogenesis.

Subunit / interactions. Microtubule inner protein component of sperm flagellar doublet microtubules. Interacts with HSP90; this interaction stabilizes and activates TSSK6. Interacts with the heat shock proteins HSPCB, HSPA8 and HSPA1A. These interactions appear to be required for TSSK6 kinase activity. Interacts with TSACC; this interaction is direct and recruits TSACC to HSP90, which is essential for kinase activity.

Subcellular location. Cytoplasm. Cytoskeleton. Flagellum axoneme. Nucleus.

Tissue specificity. Highly expressed in testis. Expressed at lower levels in colon, small intestine, ovary, prostate, thymus, spleen and peripheral blood leukocytes.

Post-translational modifications. Autophosphorylated. Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.

Similarity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

RefSeq proteins (1): NP_114426* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000719	Prot_kinase_dom	Domain
IPR008271	Ser/Thr_kinase_AS	Active_site
IPR011009	Kinase-like_dom_sf	Homologous_superfamily
IPR017441	Protein_kinase_ATP_BS	Binding_site
IPR042710	TSSK6_STKc	Domain

Pfam: PF00069

Enzyme classification (BRENDA):

• EC 2.7.11.1 — non-specific serine/threonine protein kinase (BRENDA: 71 organisms, 682 substrates, 228 inhibitors, 23 Km, 6 kcat entries)

Substrate kinetics (BRENDA)

8 substrates with measured Km, best-characterized 8. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
ATP	0.0007–0.64	11
KKRAARATSNVFA	0.013–0.045	3
PAH1 PHOSPHATIDATE PHOSPHATASE	0.0002	2
RRRLSSLRA	0.0036–0.0037	2
GTP	0.46	1
KKRAARASSNVFA	0.02	1
LYS-LYS-PHE-ASN-ARG-THR-LEU-SER-VAL-ALA	0.0093	1
MYELIN BASIC PROTEIN	0.145	1

Catalyzed reactions (Rhea), 2 shown:

• L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (RHEA:17989)
• L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (RHEA:46608)

UniProt features (7 total): binding site 2, mutagenesis site 2, chain 1, domain 1, active site 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BXA6-F1	93.76	0.86

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 135 (proton acceptor)

Ligand- & substrate-binding residues (2): 18–26; 41

Mutagenesis-validated functional residues (2):

Position	Phenotype
41	loss of kinase activity. loss of binding to tsacc.
135	loss of kinase activity. no effect of binding to tsacc.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 75 (showing top): GSE45365_CD8A_DC_VS_CD11B_DC_IFNAR_KO_MCMV_INFECTION_DN, DARWICHE_SKIN_TUMOR_PROMOTER_DN, DARWICHE_PAPILLOMA_RISK_LOW_DN, DARWICHE_PAPILLOMA_RISK_HIGH_DN, DARWICHE_SQUAMOUS_CELL_CARCINOMA_DN, GOBP_MALE_GAMETE_GENERATION, GOBP_NUCLEUS_ORGANIZATION, GOBP_CILIUM_MOVEMENT, GOBP_CILIUM_OR_FLAGELLUM_DEPENDENT_CELL_MOTILITY, TGANTCA_AP1_C, GOBP_CELLULAR_PROCESS_INVOLVED_IN_REPRODUCTION_IN_MULTICELLULAR_ORGANISM, GOBP_SPERMATID_NUCLEUS_DIFFERENTIATION, GOBP_DEVELOPMENTAL_PROCESS_INVOLVED_IN_REPRODUCTION, WEBER_METHYLATED_HCP_IN_FIBROBLAST_DN, GOCC_CYTOPLASMIC_REGION

GO Biological Process (6): protein phosphorylation (GO:0006468), spermatid development (GO:0007286), flagellated sperm motility (GO:0030317), sperm DNA condensation (GO:0035092), spermatogenesis (GO:0007283), cell differentiation (GO:0030154)

GO Molecular Function (11): magnesium ion binding (GO:0000287), protein serine/threonine kinase activity (GO:0004674), ATP binding (GO:0005524), protein-containing complex binding (GO:0044877), protein serine kinase activity (GO:0106310), nucleotide binding (GO:0000166), protein kinase activity (GO:0004672), protein binding (GO:0005515), kinase activity (GO:0016301), transferase activity (GO:0016740), metal ion binding (GO:0046872)

GO Cellular Component (8): nucleus (GO:0005634), sperm flagellum (GO:0036126), axonemal microtubule doublet inner sheath (GO:0160110), cytoplasm (GO:0005737), cytoskeleton (GO:0005856), cilium (GO:0005929), motile cilium (GO:0031514), cell projection (GO:0042995)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
protein kinase activity	2
binding	2
phosphorylation	1
protein modification process	1
germ cell development	1
spermatid differentiation	1
cilium-dependent cell motility	1
cilium movement involved in cell motility	1
sperm motility	1
chromatin organization	1
spermatid nucleus differentiation	1
developmental process involved in reproduction	1
male gamete generation	1
cellular developmental process	1
metal ion binding	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
kinase activity	1
phosphotransferase activity, alcohol group as acceptor	1
catalytic activity, acting on a protein	1
transferase activity, transferring phosphorus-containing groups	1
catalytic activity	1
cation binding	1
intracellular membrane-bounded organelle	1
9+2 motile cilium	1
axonemal doublet microtubule	1
intracellular anatomical structure	1
intracellular membraneless organelle	1
intraciliary transport particle	1
membrane-bounded organelle	1
plasma membrane bounded cell projection	1
cilium	1

Protein interactions and networks

STRING

2073 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TSSK6	TNP1	P09430	812
TSSK6	TSKS	Q9UJT2	720
TSSK6	IZUMO1	Q8IYV9	659
TSSK6	HSP90AB1	P08238	642
TSSK6	SPACA6	W5XKT8	617
TSSK6	OAZ3	Q9UMX2	615
TSSK6	CALY	Q9NYX4	603
TSSK6	SPEM1	Q8N4L4	593
TSSK6	PRM2	P04554	529
TSSK6	PLCZ1	Q86YW0	517
TSSK6	YJEFN3	A6XGL0	513
TSSK6	SPACA1	Q9HBV2	510
TSSK6	TSACC	Q96A04	507
TSSK6	DDX25	Q9UHL0	492
TSSK6	ODF1	Q14990	490

IntAct

17 interactions, top by confidence:

A	B	Type	Score
TSSK6	HSP90AA1	psi-mi:“MI:0914”(association)	0.740
TSSK6	HSP90AB1	psi-mi:“MI:0915”(physical association)	0.740
TSSK6	HSP90AB1	psi-mi:“MI:0914”(association)	0.740
TSSK6	HSP90AA1	psi-mi:“MI:0915”(physical association)	0.740
CDC37	TSSK6	psi-mi:“MI:0915”(physical association)	0.740
TSSK6	TCP1	psi-mi:“MI:0914”(association)	0.530
MAD2L1BP	KIF20A	psi-mi:“MI:0914”(association)	0.530
MLF2	TSSK6	psi-mi:“MI:0915”(physical association)	0.400
TSSK6	HSP90AB1	psi-mi:“MI:0915”(physical association)	0.400
TSSK6	PSMD2	psi-mi:“MI:0915”(physical association)	0.400
TSSK6	AARSD1	psi-mi:“MI:0915”(physical association)	0.400
TSSK6	HAX1	psi-mi:“MI:0914”(association)	0.350
HOXA5	ANKHD1-EIF4EBP3	psi-mi:“MI:0914”(association)	0.350
TSSK6	HSPA1A	psi-mi:“MI:0914”(association)	0.350

BioGRID (62): CCT2 (Affinity Capture-MS), CCT3 (Affinity Capture-MS), CCT4 (Affinity Capture-MS), CCT5 (Affinity Capture-MS), CCT6A (Affinity Capture-MS), CCT7 (Affinity Capture-MS), CCT8 (Affinity Capture-MS), FKBP5 (Affinity Capture-MS), TCP1 (Affinity Capture-MS), HSP90AA5P (Affinity Capture-MS), FKBP5 (Affinity Capture-MS), ARFGAP2 (Affinity Capture-MS), HSP90AA1 (Affinity Capture-MS), HSP90AB1 (Affinity Capture-MS), CDC37 (Affinity Capture-MS)

ESM2 similar proteins: A0A5B9GBF0, A1IVT7, A3B529, B2MVY4, O65554, O70150, O70444, P05128, P11802, P23111, P27638, P29618, P30285, P34112, P35426, P48609, P52389, P58750, P79432, P93101, Q05006, Q0U4L8, Q15759, Q1DUU8, Q2PQN9, Q2U469, Q32KY4, Q38772, Q38HL5, Q41639, Q5JLD8, Q683C9, Q69Q47, Q6ERS4, Q6P2M8, Q6SA08, Q80YP0, Q86V86, Q8C1R0, Q8TGA9

Diamond homologs: A1A5Q6, A2XFF4, A7MB74, A8XJQ6, A8XW88, B1WAS2, B1WBU5, B8BBT7, C0HKC8, C0HKC9, G1X456, O61661, O70150, O88866, O94806, P00517, P05131, P05132, P05383, P05986, P06244, P06245, P0C263, P0C264, P0C5K0, P0C5K1, P0DP15, P12370, P16912, P17612, P21137, P22612, P22694, P25321, P27791, P34099, P36887, P45894, P57058, P57059

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 13 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

GO biological processes:

GO term	Partners	Fold	FDR
protein stabilization	5	27.9×	3e-05

Disease & clinical

Clinical variants and AI predictions

ClinVar

22 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	21
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

57 predictions. Top by Δscore:

Variant	Effect	Δscore
19:19514852:CTT:C	donor_gain	0.6900
19:19514851:A:AC	donor_gain	0.6600
19:19514852:C:CC	donor_gain	0.6600
19:19514854:T:TA	donor_gain	0.5800
19:19514455:C:CT	acceptor_gain	0.5600
19:19514455:C:T	acceptor_gain	0.5600
19:19514452:CCCCG:C	acceptor_gain	0.4700
19:19514880:A:AC	donor_gain	0.4500
19:19515156:T:TA	donor_gain	0.4500
19:19514853:T:C	donor_gain	0.4300
19:19514454:CCG:C	acceptor_gain	0.4000
19:19514847:T:TA	donor_gain	0.4000
19:19514453:CCCG:C	acceptor_gain	0.3800
19:19514456:G:C	acceptor_gain	0.3800
19:19514595:C:CT	donor_gain	0.3500
19:19514596:C:CT	donor_gain	0.3300
19:19514452:C:A	donor_gain	0.3200
19:19514477:C:CT	acceptor_gain	0.3100
19:19514526:C:CA	donor_gain	0.3100
19:19514882:C:T	donor_gain	0.3100
19:19514456:G:T	acceptor_gain	0.3000
19:19514806:CGGTG:C	donor_gain	0.3000
19:19514456:G:GC	acceptor_gain	0.2900
19:19514523:ATTC:A	donor_gain	0.2900
19:19514804:CCCGG:C	donor_gain	0.2900
19:19514451:C:CA	donor_gain	0.2800
19:19514800:T:C	donor_gain	0.2800
19:19514839:TCCA:T	donor_gain	0.2800
19:19514840:CCAC:C	donor_gain	0.2800
19:19514517:T:TA	donor_gain	0.2700

AlphaMissense

1750 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:19514966:G:C	D154E	1.000
19:19514966:G:T	D154E	1.000
19:19514967:T:A	D154V	1.000
19:19515017:C:A	K137N	1.000
19:19515017:C:G	K137N	1.000
19:19515023:G:C	D135E	1.000
19:19515023:G:T	D135E	1.000
19:19515024:T:A	D135V	1.000
19:19515024:T:C	D135G	1.000
19:19515024:T:G	D135A	1.000
19:19515260:G:C	F56L	1.000
19:19515260:G:T	F56L	1.000
19:19515262:A:G	F56L	1.000
19:19515305:C:A	K41N	1.000
19:19515305:C:G	K41N	1.000
19:19514663:C:A	R255S	0.999
19:19514663:C:G	R255S	0.999
19:19514664:C:A	R255M	0.999
19:19514664:C:G	R255T	0.999
19:19514792:G:C	F212L	0.999
19:19514792:G:T	F212L	0.999
19:19514794:A:G	F212L	0.999
19:19514796:G:T	P211H	0.999
19:19514797:G:A	P211S	0.999
19:19514833:C:G	G199R	0.999
19:19514840:C:A	W196C	0.999
19:19514840:C:G	W196C	0.999
19:19514842:A:G	W196R	0.999
19:19514842:A:T	W196R	0.999
19:19514847:T:A	D194V	0.999

dbSNP variants (sampled 300 via entrez): RS1001298943 (19:19514495 T>C), RS1001708485 (19:19515179 G>A,T), RS1001916701 (19:19517372 G>A,C), RS1002309821 (19:19513893 T>C), RS1002641616 (19:19515532 A>AC), RS1002811585 (19:19514254 G>A,C), RS1003592932 (19:19516261 A>C,G), RS1003645138 (19:19516384 G>A,T), RS1004426221 (19:19516446 C>A,T), RS1004673248 (19:19516616 C>G,T), RS1005050587 (19:19517482 G>A), RS1005959845 (19:19515804 A>G,T), RS1006488550 (19:19515579 T>C), RS1007765378 (19:19514300 A>C), RS1008043324 (19:19514451 C>G,T)

Disease associations

OMIM: gene MIM:610712 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

13 associations (top):

Study	Trait	p-value
GCST002149_11	Schizophrenia	3.000000e-09
GCST002539_89	Schizophrenia	4.000000e-10
GCST006803_88	Schizophrenia	7.000000e-12
GCST007294_16	Body fat distribution (trunk fat ratio)	2.000000e-07
GCST007294_35	Body fat distribution (trunk fat ratio)	1.000000e-10
GCST007295_166	Body fat distribution (leg fat ratio)	2.000000e-11
GCST007295_22	Body fat distribution (leg fat ratio)	3.000000e-07
GCST008103_10	Bipolar disorder	1.000000e-09
GCST008115_2	Bipolar I disorder	3.000000e-09
GCST008116_4	Bipolar II disorder	4.000000e-06
GCST010002_52	Refractive error	4.000000e-29
GCST010703_335	Brain morphology (MOSTest)	3.000000e-10
GCST90016673_3	Percent liver fat	1.000000e-08

EFO canonical traits (5, from GWAS)

EFO ID	Trait name
EFO:0004341	body fat distribution
EFO:0009963	bipolar I disorder
EFO:0009964	bipolar II disorder
EFO:0004346	neuroimaging measurement
EFO:0010821	liver fat measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL3627582 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: enzyme — Testis specific kinase (TSSK) family

ChEMBL bioactivities

3 potent at pChembl≥5 of 3 total, top 3 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.95	IC50	112	nM	STAUROSPORINE
6.84	IC50	145	nM	STAUROSPORINE
6.71	IC50	193	nM	STAUROSPORINE

PubChem BioAssay actives

3 with measured affinity, of 21 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
(2S,3R,4R,6R)-3-methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one	1715164: Inhibition of human SSTK using MBP as substrate by [gamma-33P]-ATP assay	ic50	0.1120	uM

CTD chemical–gene interactions

18 total (human), top 18 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
aristolochic acid I	increases expression	1
GSK-J4	decreases expression	1
ferrous chloride	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
clothianidin	decreases expression	1
abrine	increases expression	1
(+)-JQ1 compound	decreases expression	1
Air Pollutants	increases abundance, increases expression	1
Benzo(a)pyrene	affects methylation, increases methylation	1
Cadmium	increases expression	1
Cisplatin	decreases expression	1
Folic Acid	decreases expression	1
Smoke	decreases expression	1
Urethane	affects expression	1
Valproic Acid	increases methylation	1
Okadaic Acid	decreases expression	1
Acrylamide	increases expression	1
Particulate Matter	increases expression, increases abundance	1

ChEMBL screening assays

15 unique, capped per target: 15 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL3631478	Binding	Inhibition of SSTK (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Crystal structures of human RIP2 kinase catalytic domain complexed with ATP-competitive inhibitors: Foundations for understanding inhibitor selectivity. — Bioorg Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.