TUBB8B

Summary

TUBB8B (tubulin beta 8B, HGNC:24983) is a protein-coding gene on chromosome 18p11.32, encoding Tubulin beta 8B (A6NNZ2). Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers.

Predicted to enable GTP binding activity. Predicted to be a structural constituent of cytoskeleton. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Located in intercellular bridge; microtubule; and mitotic spindle.

Source: NCBI Gene 260334 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 6 total
• Druggable target: yes — 1 molecules with ChEMBL bioactivity
• MANE Select transcript: NM_001358689

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24983
Approved symbol	TUBB8B
Name	tubulin beta 8B
Location	18p11.32
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000173213
Ensembl biotype	protein_coding
Entrez	260334

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 4 protein_coding_CDS_not_defined, 1 protein_coding

ENST00000308911, ENST00000572530, ENST00000575325, ENST00000594555, ENST00000706408

RefSeq mRNA: 5 — MANE Select: NM_001358689 NM_001358689, NM_001389609, NM_001421730, NM_001421742, NM_001421744

CCDS: CCDS86657

Canonical transcript exons

ENST00000308911 — 4 exons

Exon	Start	End
ENSE00001607222	49129	49237
ENSE00001747921	48940	49050
ENSE00002053883	47221	48447
ENSE00003624146	49501	49615

Expression profiles

Bgee: expression breadth ubiquitous, 102 present calls, max score 82.81.

Top tissues by expression

120 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	82.81	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	80.61	gold quality
left testis	UBERON:0004533	67.18	gold quality
testis	UBERON:0000473	67.11	gold quality
right testis	UBERON:0004534	66.26	gold quality
lower esophagus mucosa	UBERON:0035834	65.24	gold quality
right uterine tube	UBERON:0001302	63.31	gold quality
placenta	UBERON:0001987	55.73	gold quality
granulocyte	CL:0000094	54.96	gold quality
right adrenal gland cortex	UBERON:0035827	51.63	gold quality
pituitary gland	UBERON:0000007	50.33	gold quality
esophagus mucosa	UBERON:0002469	50.12	gold quality
fallopian tube	UBERON:0003889	49.08	gold quality
left uterine tube	UBERON:0001303	49.00	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	48.68	gold quality
adenohypophysis	UBERON:0002196	48.36	gold quality
right adrenal gland	UBERON:0001233	47.82	gold quality
cortex of kidney	UBERON:0001225	45.59	gold quality
right ovary	UBERON:0002118	45.10	gold quality
left adrenal gland	UBERON:0001234	44.91	gold quality
left adrenal gland cortex	UBERON:0035825	44.20	gold quality
vagina	UBERON:0000996	44.17	gold quality
blood	UBERON:0000178	44.13	gold quality
mucosa of stomach	UBERON:0001199	43.96	gold quality
kidney	UBERON:0002113	43.23	gold quality
superior frontal gyrus	UBERON:0002661	43.19	gold quality
adrenal gland	UBERON:0002369	43.15	gold quality
tonsil	UBERON:0002372	42.87	silver quality
prostate gland	UBERON:0002367	42.86	gold quality
ovary	UBERON:0000992	42.85	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.79

Regulation

Is transcription factor: no

Cross-species orthologs

0 orthologs

Paralogs (23): TUBG2 (ENSG00000037042), TUBE1 (ENSG00000074935), TUBA3D (ENSG00000075886), TUBB1 (ENSG00000101162), TUBB4A (ENSG00000104833), TUBD1 (ENSG00000108423), TUBA1B (ENSG00000123416), TUBA4A (ENSG00000127824), TUBG1 (ENSG00000131462), TUBB2A (ENSG00000137267), TUBB2B (ENSG00000137285), TUBA3E (ENSG00000152086), TUBA1A (ENSG00000167552), TUBA1C (ENSG00000167553), TUBB6 (ENSG00000176014), TUBAL3 (ENSG00000178462), TUBA8 (ENSG00000183785), TUBB4B (ENSG00000188229), TUBB (ENSG00000196230), TUBA3C (ENSG00000198033), TUBA4B (ENSG00000243910), TUBB3 (ENSG00000258947), TUBB8 (ENSG00000261456)

Protein

Protein identifiers

Tubulin beta 8B — A6NNZ2 (reviewed: A6NNZ2)

All UniProt accessions (1): A6NNZ2

UniProt curated annotations — full annotation on UniProt →

Function. Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Subunit / interactions. Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Subcellular location. Cytoplasm. Cytoskeleton.

Post-translational modifications. Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Domain organisation. The MREI motif is common among all beta-tubulin isoforms and may be critical for tubulin autoregulation.

Similarity. Belongs to the tubulin family.

RefSeq proteins (4): NP_001345618, NP_001408659, NP_001408671, NP_001408673 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000217	Tubulin	Family
IPR002453	Beta_tubulin	Family
IPR003008	Tubulin_FtsZ_GTPase	Domain
IPR008280	Tub_FtsZ_C	Homologous_superfamily
IPR013838	Beta-tubulin_BS	Binding_site
IPR017975	Tubulin_CS	Conserved_site
IPR018316	Tubulin/FtsZ_2-layer-sand-dom	Domain
IPR023123	Tubulin_C	Homologous_superfamily
IPR036525	Tubulin/FtsZ_GTPase_sf	Homologous_superfamily
IPR037103	Tubulin/FtsZ-like_C	Homologous_superfamily

Pfam: PF00091, PF03953

UniProt features (15 total): binding site 9, modified residue 2, chain 1, region of interest 1, short sequence motif 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A6NNZ2-F1	91.38	0.80

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (9): 144; 204; 226; 11; 69; 69; 138; 142; 143

Post-translational modifications (2): 172, 436

Function

Pathways and Gene Ontology

Reactome pathways

77 pathways

ID	Pathway
R-HSA-1445148	Translocation of SLC2A4 (GLUT4) to the plasma membrane
R-HSA-190840	Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane
R-HSA-190861	Gap junction assembly
R-HSA-2132295	MHC class II antigen presentation
R-HSA-2467813	Separation of Sister Chromatids
R-HSA-2500257	Resolution of Sister Chromatid Cohesion
R-HSA-3371497	HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand
R-HSA-380320	Recruitment of NuMA to mitotic centrosomes
R-HSA-437239	Recycling pathway of L1
R-HSA-5620920	Cargo trafficking to the periciliary membrane
R-HSA-5626467	RHO GTPases activate IQGAPs
R-HSA-5663220	RHO GTPases Activate Formins
R-HSA-6807878	COPI-mediated anterograde transport
R-HSA-6811434	COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-6811436	COPI-independent Golgi-to-ER retrograde traffic
R-HSA-68877	Mitotic Prometaphase
R-HSA-8852276	The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8955332	Carboxyterminal post-translational modifications of tubulin
R-HSA-9609690	HCMV Early Events
R-HSA-9609736	Assembly and cell surface presentation of NMDA receptors
R-HSA-9619483	Activation of AMPK downstream of NMDARs
R-HSA-9646399	Aggrephagy
R-HSA-9648025	EML4 and NUDC in mitotic spindle formation
R-HSA-9668328	Sealing of the nuclear envelope (NE) by ESCRT-III
R-HSA-983189	Kinesins
R-HSA-9833482	PKR-mediated signaling
R-HSA-5617833	Cilium Assembly
R-HSA-109582	Hemostasis
R-HSA-112314	Neurotransmitter receptors and postsynaptic signal transmission
R-HSA-112315	Transmission across Chemical Synapses

MSigDB gene sets: 84 (showing top): REACTOME_ADAPTIVE_IMMUNE_SYSTEM, REACTOME_CYTOKINE_SIGNALING_IN_IMMUNE_SYSTEM, REACTOME_MEMBRANE_TRAFFICKING, GOBP_MITOTIC_CELL_CYCLE, REACTOME_GAP_JUNCTION_ASSEMBLY, REACTOME_TRANSMISSION_ACROSS_CHEMICAL_SYNAPSES, GOCC_SPINDLE, GOCC_MITOTIC_SPINDLE, GOCC_INTERCELLULAR_BRIDGE, GOMF_GTPASE_ACTIVITY, GOMF_HYDROLASE_ACTIVITY_ACTING_ON_ACID_ANHYDRIDES, GOMF_STRUCTURAL_CONSTITUENT_OF_CYTOSKELETON, GOMF_STRUCTURAL_MOLECULE_ACTIVITY, REACTOME_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, GOBP_MICROTUBULE_CYTOSKELETON_ORGANIZATION

GO Biological Process (3): microtubule cytoskeleton organization (GO:0000226), mitotic cell cycle (GO:0000278), microtubule-based process (GO:0007017)

GO Molecular Function (5): GTPase activity (GO:0003924), structural constituent of cytoskeleton (GO:0005200), GTP binding (GO:0005525), metal ion binding (GO:0046872), nucleotide binding (GO:0000166)

GO Cellular Component (7): cytoplasm (GO:0005737), microtubule (GO:0005874), microtubule cytoskeleton (GO:0015630), intercellular bridge (GO:0045171), extracellular exosome (GO:0070062), mitotic spindle (GO:0072686), cytoskeleton (GO:0005856)

Reactome top-level categories

Rollup of top-17 pathways:

Category	Pathways
Mitotic Prometaphase	2
RHO GTPase Effectors	2
Golgi-to-ER retrograde transport	2
Membrane Trafficking	1
Transport of connexons to the plasma membrane	1
Gap junction trafficking	1
Adaptive Immune System	1
Mitotic Anaphase	1
Cellular responses to stress	1
L1CAM interactions	1
Assembly of the 9+0 primary cilium	1
ER to Golgi Anterograde Transport	1
M Phase	1
G2/M Transition	1
Post-translational protein modification	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cytoskeleton organization	2
cytoskeleton	2
cellular anatomical structure	2
microtubule-based process	1
cell cycle	1
mitotic nuclear division	1
cellular process	1
ribonucleoside triphosphate phosphatase activity	1
structural molecule activity	1
guanyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
cation binding	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
intracellular anatomical structure	1
microtubule cytoskeleton	1
polymeric cytoskeletal fiber	1
extracellular vesicle	1
spindle	1
intracellular membraneless organelle	1

Protein interactions and networks

STRING

2586 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TUBB8B	GLUD2	P49448	255
TUBB8B	CAMKK2	Q96RR4	215
TUBB8B	ATP2C2	O75185	211
TUBB8B	KATNAL2	Q8IYT4	204
TUBB8B	SCNN1G	P51170	185
TUBB8B	GLIS1	Q8NBF1	183
TUBB8B	EPB41L4B	Q9H329	174
TUBB8B	IDH1	O75874	173
TUBB8B	MCM7	P33993	172
TUBB8B	EEF2	P13639	168
TUBB8B	TBCD	Q9BTW9	168
TUBB8B	TBCA	O75347	167
TUBB8B	CRAT	P43155	167
TUBB8B	MUC12	Q9UKN1	167
TUBB8B	ESPL1	Q14674	166
TUBB8B	GTF2H4	Q92759	166

IntAct

8 interactions, top by confidence:

A	B	Type	Score
BACE1	TUBB8B	psi-mi:“MI:0914”(association)	0.350
TUBB	POTEF	psi-mi:“MI:0914”(association)	0.350
TUBB4B	POTEF	psi-mi:“MI:0914”(association)	0.350
TUBB3	TUBB8B	psi-mi:“MI:0914”(association)	0.350
ICAM1	TUBB8B	psi-mi:“MI:0914”(association)	0.350
OPG065	TUBB8B	psi-mi:“MI:0914”(association)	0.350
	ESYT2	psi-mi:“MI:0914”(association)	0.350

ESM2 similar proteins: A6NNZ2, P02554, P04350, P07437, P09206, P09244, P09652, P11833, P11857, P14643, P18025, P30156, P61857, P61858, P68371, P69893, P69895, P69897, P83130, P99024, Q08115, Q13509, Q24560, Q27U48, Q2HJ81, Q2KJD0, Q2T9S0, Q3MHM5, Q3ZBU7, Q3ZCM7, Q4QRB4, Q4R4X8, Q5R943, Q60HC2, Q6GLE7, Q6P9T8, Q6VAF4, Q767L7, Q7JJU6, Q7KQL5

Diamond homologs: A2AQ07, A6NNZ2, O04386, O17449, O44388, P02554, P04350, P04690, P07436, P07437, P08841, P09203, P09206, P09207, P09244, P09652, P09653, P10876, P10878, P11482, P11833, P11857, P12456, P13602, P14643, P18241, P20365, P22852, P30883, P32882, P33188, P34108, P36221, P37832, P41352, P41387, P41937, P46265, P50261, P50262

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

6 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	6
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

2966 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
18:47543:G:C	F394L	0.905
18:47543:G:T	F394L	0.905
18:47545:A:G	F394L	0.905
18:48326:G:C	F133L	0.880
18:48326:G:T	F133L	0.880
18:48328:A:G	F133L	0.880
18:47999:G:C	F242L	0.878
18:47999:G:T	F242L	0.878
18:48001:A:G	F242L	0.878
18:49235:G:C	F20L	0.872
18:49235:G:T	F20L	0.872
18:49237:A:G	F20L	0.872
18:48224:G:C	F167L	0.868
18:48224:G:T	F167L	0.868
18:48226:A:G	F167L	0.868
18:47570:A:C	F385L	0.867
18:47570:A:T	F385L	0.867
18:47572:A:G	F385L	0.867
18:47774:G:C	F317L	0.848
18:47774:G:T	F317L	0.848
18:47776:A:G	F317L	0.848
18:47501:G:C	F408L	0.847
18:47501:G:T	F408L	0.847
18:47503:A:G	F408L	0.847
18:48221:G:C	S168R	0.845
18:48221:G:T	S168R	0.845
18:48223:T:G	S168R	0.845
18:48962:G:C	F85L	0.845
18:48962:G:T	F85L	0.845
18:48964:A:G	F85L	0.845

dbSNP variants (sampled 300 via entrez): RS1000144866 (18:51456 T>C), RS1000225823 (18:66679 G>C), RS1000248061 (18:72186 A>C), RS1000426207 (18:50651 A>T), RS1000452976 (18:51666 C>T), RS1000543173 (18:57827 A>C), RS1000563209 (18:67844 C>T), RS1000633406 (18:73338 C>A,T), RS1000703045 (18:72400 G>C), RS1000828684 (18:57573 C>G), RS1000984088 (18:73224 T>A,C,G), RS1001136700 (18:67993 C>A,G), RS1001226726 (18:65392 CT>C), RS1001308133 (18:70889 A>G), RS1001345248 (18:63277 G>A)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL3832942 (PROTEIN FAMILY), CHEMBL6066847 (PROTEIN-PROTEIN INTERACTION)

Molecules with ChEMBL bioactivity

1 molecules (phase ≥1), by development phase (incl. off-target/promiscuous compounds). Patent mentions across the top 20 by phase: 14,987 (via chembl_molecule»patent_compound — counts attach to the compound, not the gene–compound relationship, so off-target/promiscuous molecules can dominate).

Molecule	Name	Phase	Patents
CHEMBL364713	NOSCAPINE	4	14,987

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

11 potent at pChembl≥5 of 11 total, top 11 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.67	Kd	21.5	nM	CHEMBL4797381
7.60	IC50	25	nM	CHEMBL552462
7.43	Kd	36.9	nM	CHEMBL4756812
6.96	IC50	108.4	nM	CHEMBL5206193
6.88	Kd	133	nM	CHEMBL4751205
6.88	Kd	132	nM	CHEMBL4797299
6.43	Kd	374.3	nM	CHEMBL4747307
6.24	Kd	579	nM	NOSCAPINE
5.63	IC50	2320	nM	CHEMBL5197309
5.26	IC50	5510	nM	CHEMBL5276251
5.08	IC50	8400	nM	CHEMBL5186583

PubChem BioAssay actives

11 with measured affinity, of 225 total; 11 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
(3S)-3-[(5R)-6-[[1-(4-fluorophenyl)triazol-4-yl]methyl]-4-methoxy-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isoquinolin-5-yl]-6,7-dimethoxy-3H-2-benzofuran-1-one	1675128: Binding affinity to CM5 chip immobilized beta tubulin (unknown origin) assessed as thermodynamic constants by SPR assay	kd	0.0215	uM
[4-amino-2-(4-methoxyanilino)-1,3-thiazol-5-yl]-(3,4-dimethoxyphenyl)methanone	1674862: Binding affinity to beta tubulin in HEK293 cells assessed as disruption of microtubule polymerization by ITDRF-CETSA assay	ic50	0.0250	uM
(3S)-3-[(5R)-9-bromo-6-[[1-(4-bromophenyl)triazol-4-yl]methyl]-4-methoxy-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isoquinolin-5-yl]-6,7-dimethoxy-3H-2-benzofuran-1-one	1675128: Binding affinity to CM5 chip immobilized beta tubulin (unknown origin) assessed as thermodynamic constants by SPR assay	kd	0.0369	uM
N-[2-[[(E)-(2,4-dihydroxyphenyl)methylideneamino]carbamoyl]phenyl]furan-2-carboxamide	1925486: Inhibition of beta-tubulin polymerization (unknown origin) by ELISA	ic50	0.1084	uM
(3S)-3-[(5R)-9-bromo-6-[[1-(2-hydroxybutyl)triazol-4-yl]methyl]-4-methoxy-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isoquinolin-5-yl]-6,7-dimethoxy-3H-2-benzofuran-1-one	1675128: Binding affinity to CM5 chip immobilized beta tubulin (unknown origin) assessed as thermodynamic constants by SPR assay	kd	0.1320	uM
(3S)-3-[(5R)-6-[(1-benzyltriazol-4-yl)methyl]-4-methoxy-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isoquinolin-5-yl]-6,7-dimethoxy-3H-2-benzofuran-1-one	1675128: Binding affinity to CM5 chip immobilized beta tubulin (unknown origin) assessed as thermodynamic constants by SPR assay	kd	0.1330	uM
(3S)-3-[(5R)-9-bromo-6-[[1-(3,4-dichlorophenyl)triazol-4-yl]methyl]-4-methoxy-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isoquinolin-5-yl]-6,7-dimethoxy-3H-2-benzofuran-1-one	1675128: Binding affinity to CM5 chip immobilized beta tubulin (unknown origin) assessed as thermodynamic constants by SPR assay	kd	0.3743	uM
(3S)-6,7-dimethoxy-3-[(5R)-4-methoxy-6-methyl-7,8-dihydro-5H-[1,3]dioxolo[4,5-g]isoquinolin-5-yl]-3H-2-benzofuran-1-one	1675128: Binding affinity to CM5 chip immobilized beta tubulin (unknown origin) assessed as thermodynamic constants by SPR assay	kd	0.5790	uM
4-[(1,3-dioxoisoindol-2-yl)methyl]-N-[4-[(E)-3-(3,4,5-trimethylphenyl)prop-2-enoyl]phenyl]benzamide	1847784: Inhibition of beta-tubulin polymerization in human MCF7 cells using TMB as substrate incubated for 2 hrs by spectrophotometry analysis	ic50	2.3200	uM
(E)-1-(5-bromo-2-phenyl-1-benzofuran-7-yl)-3-(4-fluorophenyl)prop-2-en-1-one	1925479: Inhibition of beta-tubulin polymerization (unknown origin) measured every 3 secs for 1 hr by spectrofluorometer	ic50	5.5100	uM
(E)-1-[4-[(1-ethyl-[1,2,4]triazolo[4,3-a]quinoxalin-4-yl)amino]phenyl]-3-(3,4,5-trimethoxyphenyl)prop-2-en-1-one	1925477: Inhibition of beta-tubulin polymerization in human HCT-116 cells using TMB substrate by ELISA	ic50	8.4000	uM

CTD chemical–gene interactions

1 total (human), top 1 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
epigallocatechin gallate	increases expression	1

ChEMBL screening assays

141 unique, capped per target: 141 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL1175683	Binding	inhibition of beta tubulin acylation in human M21 cells assessed as beta-tubulin band migration by Western blotting	Synthesis, antiproliferative activity evaluation and structure-activity relationships of novel aromatic urea and amide analogues of N-phenyl-N’-(2-chloroethyl)ureas. — Eur J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.