TWF2

Summary

TWF2 (twinfilin actin binding protein 2, HGNC:9621) is a protein-coding gene on chromosome 3p21.2, encoding Twinfilin-2 (Q6IBS0). Actin-binding protein involved in motile and morphological processes.

The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.

Source: NCBI Gene 11344 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 48 total
• Druggable target: yes
• MANE Select transcript: NM_007284

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:9621
Approved symbol	TWF2
Name	twinfilin actin binding protein 2
Location	3p21.2
Locus type	gene with protein product
Status	Approved
Aliases	A6RP, A6r
Ensembl gene	ENSG00000247596
Ensembl biotype	protein_coding
OMIM	607433
Entrez	11344

Gene structure

Transcript identifiers

Ensembl transcripts: 25 — 12 protein_coding, 9 retained_intron, 3 nonsense_mediated_decay, 1 protein_coding_CDS_not_defined

ENST00000305533, ENST00000472755, ENST00000499914, ENST00000676552, ENST00000676800, ENST00000676988, ENST00000676989, ENST00000677058, ENST00000677127, ENST00000678330, ENST00000678352, ENST00000678549, ENST00000678681, ENST00000678700, ENST00000678838, ENST00000678882, ENST00000679180, ENST00000679296, ENST00000863526, ENST00000863527, ENST00000863528, ENST00000913256, ENST00000913257, ENST00000970732, ENST00000970733

RefSeq mRNA: 1 — MANE Select: NM_007284 NM_007284

CCDS: CCDS2849

Canonical transcript exons

ENST00000305533 — 9 exons

Exon	Start	End
ENSE00001125151	52228612	52229201
ENSE00001931654	52238992	52239158
ENSE00003459274	52229661	52229782
ENSE00003462404	52231444	52231539
ENSE00003490280	52231127	52231231
ENSE00003506265	52235029	52235106
ENSE00003579514	52230870	52230995
ENSE00003615482	52229920	52230070
ENSE00003654688	52231944	52232122

Expression profiles

Bgee: expression breadth ubiquitous, 275 present calls, max score 97.98.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 53.5881 / max 269.4716, expressed in 1821 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
42411	53.3370	1821
42409	0.2398	48
42410	0.0112	8

Top tissues by expression

291 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
apex of heart	UBERON:0002098	97.98	gold quality
hindlimb stylopod muscle	UBERON:0004252	97.84	gold quality
granulocyte	CL:0000094	97.36	gold quality
gastrocnemius	UBERON:0001388	97.26	gold quality
monocyte	CL:0000576	96.90	gold quality
muscle of leg	UBERON:0001383	96.56	gold quality
gluteal muscle	UBERON:0002000	96.49	gold quality
leukocyte	CL:0000738	96.43	gold quality
mononuclear cell	CL:0000842	96.42	gold quality
muscle organ	UBERON:0001630	95.10	gold quality
heart left ventricle	UBERON:0002084	95.10	gold quality
cardiac ventricle	UBERON:0002082	94.90	gold quality
triceps brachii	UBERON:0001509	94.79	gold quality
blood	UBERON:0000178	93.78	gold quality
mucosa of transverse colon	UBERON:0004991	93.77	gold quality
stromal cell of endometrium	CL:0002255	93.59	gold quality
body of tongue	UBERON:0011876	93.16	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	93.10	gold quality
heart	UBERON:0000948	92.68	gold quality
skeletal muscle tissue	UBERON:0001134	92.66	gold quality
lymph node	UBERON:0000029	92.65	gold quality
spleen	UBERON:0002106	92.59	gold quality
prefrontal cortex	UBERON:0000451	92.55	gold quality
right atrium auricular region	UBERON:0006631	92.54	gold quality
right coronary artery	UBERON:0001625	92.33	gold quality
descending thoracic aorta	UBERON:0002345	92.33	gold quality
right frontal lobe	UBERON:0002810	92.27	gold quality
thoracic aorta	UBERON:0001515	92.08	gold quality
right hemisphere of cerebellum	UBERON:0014890	92.06	gold quality
ascending aorta	UBERON:0001496	92.00	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.99

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

8 targeting TWF2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-12118	100.00	65.88	1270
HSA-MIR-3173-3P	99.98	66.49	1217
HSA-MIR-6891-5P	99.98	66.53	1372
HSA-MIR-3120-3P	99.54	70.28	2669
HSA-MIR-1207-5P	99.49	69.11	2983
HSA-MIR-4763-3P	99.10	67.83	2649
HSA-MIR-3194-5P	96.80	64.90	1027
HSA-MIR-7108-5P	96.42	66.17	598

Literature-anchored findings (GeneRIF, showing 2)

• twinfilin-2 is a protein that is involved in neurite outgrowth (PMID:17910947)
• Cross-Platform Synaptic Network Analysis of Human Entorhinal Cortex Identifies TWF2 as a Modulator of Dendritic Spine Length. (PMID:37055180)

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	twf2b	ENSDARG00000009727
danio_rerio	twf2a	ENSDARG00000094792
mus_musculus	Twf2	ENSMUSG00000023277
rattus_norvegicus	Twf2	ENSRNOG00000048915
drosophila_melanogaster	twf	FBGN0038206
caenorhabditis_elegans		WBGENE00018187

Paralogs (1): TWF1 (ENSG00000151239)

Protein

Protein identifiers

Twinfilin-2 — Q6IBS0 (reviewed: Q6IBS0)

Alternative names: A6-related protein, Protein tyrosine kinase 9-like, Twinfilin-1-like protein

All UniProt accessions (7): A0A7I2V2U8, A0A7I2V3T5, A0A7I2V5B1, A0A7I2V5D3, A0A7I2YQM6, D6RG15, Q6IBS0

UniProt curated annotations — full annotation on UniProt →

Function. Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia.

Subunit / interactions. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A.

Subcellular location. Cytoplasm. Cytoskeleton. Perinuclear region. Cell projection. Stereocilium.

Tissue specificity. Ubiquitously expressed (at protein level).

Post-translational modifications. In vitro, phosphorylated by PRKCZ, CK2 and SRC.

Similarity. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.

RefSeq proteins (1): NP_009215* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002108	ADF-H	Domain
IPR028458	Twinfilin	Family
IPR029006	ADF-H/Gelsolin-like_dom_sf	Homologous_superfamily

Pfam: PF00241

UniProt features (39 total): helix 13, strand 11, modified residue 4, sequence variant 3, domain 2, initiator methionine 1, chain 1, sequence conflict 1, turn 1, region of interest 1, compositionally biased region 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
2VAC	X-RAY DIFFRACTION	1.7
2W0I	X-RAY DIFFRACTION	1.8

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q6IBS0-F1	91.41	0.81

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (4): 2, 14, 309, 349

Function

Pathways and Gene Ontology

Reactome pathways

2 pathways

ID	Pathway
R-HSA-9662360	Sensory processing of sound by inner hair cells of the cochlea
R-HSA-9662361	Sensory processing of sound by outer hair cells of the cochlea

MSigDB gene sets: 284 (showing top): GOBP_NEGATIVE_REGULATION_OF_PROTEIN_CONTAINING_COMPLEX_ASSEMBLY, GOBP_REGULATION_OF_PROTEIN_POLYMERIZATION, GOBP_NEURON_PROJECTION_EXTENSION, GOBP_CELLULAR_RESPONSE_TO_LIPID, GOBP_REGULATION_OF_DEVELOPMENTAL_GROWTH, GOBP_NEGATIVE_REGULATION_OF_PROTEIN_POLYMERIZATION, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GOBP_BARBED_END_ACTIN_FILAMENT_CAPPING, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_UP, GOBP_GROWTH, IVANOVA_HEMATOPOIESIS_LATE_PROGENITOR, THEILGAARD_NEUTROPHIL_AT_SKIN_WOUND_DN, GOBP_NEGATIVE_REGULATION_OF_ACTIN_FILAMENT_DEPOLYMERIZATION, GOBP_NEUROGENESIS, GOBP_REGULATION_OF_NERVOUS_SYSTEM_DEVELOPMENT

GO Biological Process (12): regulation of lamellipodium assembly (GO:0010591), positive regulation of lamellipodium assembly (GO:0010592), positive regulation of neuron projection development (GO:0010976), cell projection organization (GO:0030030), actin filament depolymerization (GO:0030042), negative regulation of actin filament polymerization (GO:0030837), regulation of microvillus length (GO:0032532), regulation of actin cytoskeleton organization (GO:0032956), positive regulation of axon extension (GO:0045773), barbed-end actin filament capping (GO:0051016), cellular response to retinoic acid (GO:0071300), cellular response to growth factor stimulus (GO:0071363)

GO Molecular Function (10): RNA binding (GO:0003723), actin monomer binding (GO:0003785), protein kinase C binding (GO:0005080), ATP binding (GO:0005524), phosphatidylinositol-4,5-bisphosphate binding (GO:0005546), cadherin binding (GO:0045296), actin filament binding (GO:0051015), actin binding (GO:0003779), protein binding (GO:0005515), anion binding (GO:0043168)

GO Cellular Component (11): cytoplasm (GO:0005737), actin filament (GO:0005884), myofibril (GO:0030016), lamellipodium (GO:0030027), filopodium (GO:0030175), growth cone (GO:0030426), stereocilium (GO:0032420), perinuclear region of cytoplasm (GO:0048471), extracellular exosome (GO:0070062), cytoskeleton (GO:0005856), cell projection (GO:0042995)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Sensory processing of sound	2

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
lamellipodium assembly	2
actin binding	2
actin-based cell projection	2
regulation of plasma membrane bounded cell projection assembly	1
regulation of lamellipodium organization	1
regulation of lamellipodium assembly	1
positive regulation of plasma membrane bounded cell projection assembly	1
positive regulation of lamellipodium organization	1
regulation of neuron projection development	1
neuron projection development	1
positive regulation of cell projection organization	1
cellular component organization	1
actin polymerization or depolymerization	1
protein depolymerization	1
actin filament polymerization	1
regulation of actin filament polymerization	1
negative regulation of protein polymerization	1
negative regulation of cytoskeleton organization	1
negative regulation of supramolecular fiber organization	1
regulation of microvillus organization	1
regulation of cell projection size	1
actin cytoskeleton organization	1
regulation of actin filament-based process	1
regulation of cytoskeleton organization	1
positive regulation of cell growth	1
regulation of axon extension	1
positive regulation of developmental growth	1
axon extension	1
positive regulation of axonogenesis	1
actin filament capping	1
response to retinoic acid	1
cellular response to lipid	1
cellular response to oxygen-containing compound	1
response to growth factor	1
cellular response to endogenous stimulus	1
nucleic acid binding	1
protein kinase binding	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1

Protein interactions and networks

STRING

1148 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
TWF2	EPS8L2	Q9H6S3	766
TWF2	GSN	P06396	763
TWF2	PFN4	Q8NHR9	742
TWF2	TMOD4	Q9NZQ9	733
TWF2	SCIN	Q9Y6U3	732
TWF2	CFL1	P23528	731
TWF2	CFL2	Q9Y281	727
TWF2	MYO15A	Q9UKN7	718
TWF2	PFN3	P60673	680
TWF2	PFN1	P07737	656
TWF2	MYO7A	P78427	646
TWF2	ESPN	B1AK53	626
TWF2	EPS8	Q12929	625
TWF2	WHRN	Q9P202	604
TWF2	DBN1	Q16643	568

IntAct

86 interactions, top by confidence:

A	B	Type	Score
KHDRBS2	KHDRBS3	psi-mi:“MI:0914”(association)	0.800
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
TWF2	CAP2	psi-mi:“MI:0914”(association)	0.640
BCL7A	ARID1A	psi-mi:“MI:0914”(association)	0.640
BCL7C	ARID1A	psi-mi:“MI:0914”(association)	0.640
CAPZA2	CNOT1	psi-mi:“MI:0914”(association)	0.640
CAPZB	CNOT1	psi-mi:“MI:0914”(association)	0.640
PDCD2L	PRMT3	psi-mi:“MI:0914”(association)	0.530
TWF2	TERF2IP	psi-mi:“MI:0915”(physical association)	0.510
DDX21	MED19	psi-mi:“MI:2364”(proximity)	0.480
TWF2	HMGN2	psi-mi:“MI:0915”(physical association)	0.400
TWF2	POT1	psi-mi:“MI:0915”(physical association)	0.370
FOXJ1	TWF2	psi-mi:“MI:0915”(physical association)	0.370
TWF2	ILK	psi-mi:“MI:0915”(physical association)	0.370
TWF2	ARHGDIA	psi-mi:“MI:0915”(physical association)	0.370
MYO1C	PLEKHG3	psi-mi:“MI:0914”(association)	0.350
Actb		psi-mi:“MI:0914”(association)	0.350
LIMA1	PLEKHG3	psi-mi:“MI:0914”(association)	0.350
Coro1c	PLEKHG3	psi-mi:“MI:0914”(association)	0.350
SYNPO	LMO7	psi-mi:“MI:0914”(association)	0.350
MYH9	NAP1L1	psi-mi:“MI:0914”(association)	0.350
PLEKHA7	PLEKHG3	psi-mi:“MI:0914”(association)	0.350
PRNP	WDR91	psi-mi:“MI:0914”(association)	0.350
SCARB2	PLEKHG3	psi-mi:“MI:0914”(association)	0.350
LRRK2		psi-mi:“MI:0914”(association)	0.350

BioGRID (128): TWF2 (Co-fractionation), TWF2 (Affinity Capture-MS), TWF2 (Affinity Capture-MS), TWF2 (Affinity Capture-MS), TWF2 (Affinity Capture-MS), TWF2 (Affinity Capture-MS), TWF2 (Affinity Capture-MS), TWF2 (Affinity Capture-MS), TWF2 (Proximity Label-MS), CAP2 (Affinity Capture-MS), ACTA2 (Affinity Capture-MS), CAP1 (Affinity Capture-MS), ACTBL2 (Affinity Capture-MS), TWF2 (Affinity Capture-MS), ACTB (Affinity Capture-MS)

ESM2 similar proteins: C4LVG4, P0CM06, P0CM07, P18359, P30174, P37167, P45593, P45594, P45695, P60981, P60982, P78929, Q03048, Q0DLA3, Q12792, Q17A58, Q298X4, Q39250, Q4P6E9, Q56JV6, Q5E9D5, Q5I082, Q5R7N2, Q5RFH1, Q5RJR2, Q5ZM35, Q640W2, Q67ZM4, Q68F50, Q6C0Y0, Q6CQ22, Q6EUH7, Q6FV81, Q6GMH3, Q6IBS0, Q6NX11, Q759P0, Q7M0E3, Q7QG28, Q7XSN9

Diamond homologs: C4LVG4, O15902, O49606, P0CM06, P0CM07, P0DJ26, P0DJ27, P10668, P18359, P18760, P21566, P23528, P30174, P30175, P37167, P45591, P45592, P45593, P45594, P45695, P46251, P60981, P60982, P78929, P86293, Q03048, Q07749, Q07750, Q0D744, Q0DLA3, Q10P87, Q12792, Q148F1, Q2QLT8, Q337A5, Q39250, Q39251, Q41764, Q43694, Q4I963

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 98 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Formation of the canonical BAF (cBAF) complex	5	46.6×	9e-06
Formation of neuronal progenitor and neuronal BAF (npBAF and nBAF)	5	33.6×	3e-05
Sensory processing of sound	7	31.8×	6e-07
Regulation of MITF-M-dependent genes involved in pigmentation	5	19.5×	3e-04
Sensory processing of sound by inner hair cells of the cochlea	7	16.8×	2e-05
Sensory processing of sound by outer hair cells of the cochlea	5	15.0×	8e-04
Regulation of actin dynamics for phagocytic cup formation	5	13.5×	1e-03
MITF-M-dependent gene expression	5	13.3×	1e-03

GO biological processes:

GO term	Partners	Fold	FDR
regulation of G0 to G1 transition	5	38.3×	8e-05
regulation of nucleotide-excision repair	5	34.2×	8e-05
regulation of mitotic metaphase/anaphase transition	5	28.2×	1e-04
cell motility	5	22.8×	3e-04
positive regulation of double-strand break repair	5	19.5×	5e-04
regulation of G1/S transition of mitotic cell cycle	5	17.4×	8e-04
cell morphogenesis	5	8.9×	1e-02
chromatin remodeling	10	8.3×	8e-05

Disease & clinical

Clinical variants and AI predictions

ClinVar

48 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	37
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2081 predictions. Top by Δscore:

Variant	Effect	Δscore
3:52229079:ATGG:A	donor_gain	1.0000
3:52229079:ATGGC:A	donor_gain	1.0000
3:52229198:CAAT:C	acceptor_gain	1.0000
3:52229202:C:CC	acceptor_gain	1.0000
3:52229655:GCCTA:G	donor_loss	1.0000
3:52229656:CCTA:C	donor_loss	1.0000
3:52229657:CTA:C	donor_loss	1.0000
3:52229657:CTACT:C	donor_loss	1.0000
3:52229658:TA:T	donor_loss	1.0000
3:52229659:A:AC	donor_gain	1.0000
3:52229659:AC:A	donor_loss	1.0000
3:52229659:ACTTT:A	donor_gain	1.0000
3:52229660:C:CA	donor_gain	1.0000
3:52229660:CT:C	donor_gain	1.0000
3:52229660:CTT:C	donor_gain	1.0000
3:52229660:CTTT:C	donor_gain	1.0000
3:52229660:CTTTC:C	donor_gain	1.0000
3:52229663:T:A	donor_gain	1.0000
3:52229686:T:TA	donor_gain	1.0000
3:52229764:G:T	acceptor_gain	1.0000
3:52229778:GAACA:G	acceptor_gain	1.0000
3:52229779:AACA:A	acceptor_gain	1.0000
3:52229780:ACA:A	acceptor_gain	1.0000
3:52229780:ACACT:A	acceptor_loss	1.0000
3:52229781:CA:C	acceptor_gain	1.0000
3:52229781:CAC:C	acceptor_gain	1.0000
3:52229782:ACTGT:A	acceptor_loss	1.0000
3:52229783:C:CC	acceptor_gain	1.0000
3:52229784:T:G	acceptor_loss	1.0000
3:52229916:TCA:T	donor_loss	1.0000

AlphaMissense

2285 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:52229734:A:G	M270T	1.000
3:52231979:A:G	W83R	1.000
3:52231979:A:T	W83R	1.000
3:52229109:C:A	K325N	0.999
3:52229109:C:G	K325N	0.999
3:52229115:G:C	F323L	0.999
3:52229115:G:T	F323L	0.999
3:52229117:A:G	F323L	0.999
3:52229731:A:G	L271P	0.999
3:52229733:C:A	M270I	0.999
3:52229733:C:G	M270I	0.999
3:52229733:C:T	M270I	0.999
3:52229734:A:C	M270R	0.999
3:52229734:A:T	M270K	0.999
3:52231149:A:G	L154P	0.999
3:52231454:C:T	G123E	0.999
3:52231511:G:T	T104K	0.999
3:52231514:G:T	A103D	0.999
3:52231515:C:G	A103P	0.999
3:52231517:G:T	A102E	0.999
3:52231525:C:A	M99I	0.999
3:52231525:C:G	M99I	0.999
3:52231525:C:T	M99I	0.999
3:52231526:A:C	M99R	0.999
3:52231528:C:A	K98N	0.999
3:52231528:C:G	K98N	0.999
3:52231530:T:C	K98E	0.999
3:52231964:A:G	W88R	0.999
3:52231964:A:T	W88R	0.999
3:52229147:G:C	H313D	0.998

dbSNP variants (sampled 300 via entrez): RS1000068381 (3:52235402 A>G), RS1000390993 (3:52232251 C>A,T), RS1001114664 (3:52235084 G>T), RS1001221291 (3:52229407 C>T), RS1001493418 (3:52230460 C>T), RS1002013580 (3:52238993 G>A), RS1002069504 (3:52233577 C>A), RS1002123525 (3:52233756 A>G), RS1002171806 (3:52236265 C>G), RS1002224326 (3:52236458 C>G), RS1002364426 (3:52238817 C>G), RS1002505685 (3:52237532 A>T), RS1002557991 (3:52237940 C>T), RS1002836792 (3:52236039 A>G,T), RS1002887073 (3:52230708 G>A)

Disease associations

OMIM: gene MIM:607433 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST001241_15	Bipolar disorder	2.000000e-06
GCST002149_14	Schizophrenia	1.000000e-08
GCST004521_123	Autism spectrum disorder or schizophrenia	3.000000e-12
GCST004521_201	Autism spectrum disorder or schizophrenia	4.000000e-08

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067403 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: enzyme — Twinfilin subfamily

CTD chemical–gene interactions

46 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects expression, affects cotreatment, increases methylation, decreases expression	3
Benzo(a)pyrene	increases expression, increases methylation	2
Cisplatin	affects cotreatment, increases expression	2
Smoke	decreases expression	2
Tobacco Smoke Pollution	affects expression, increases expression	2
Tretinoin	increases expression	2
Aflatoxin B1	increases expression	2
bisphenol F	increases expression	1
2,4,6-tribromophenol	increases expression	1
testosterone enanthate	affects expression	1
triphenyl phosphate	affects expression	1
decabromobiphenyl ether	increases expression	1
sodium arsenite	decreases expression, increases abundance	1
tetrabromobisphenol A	increases expression	1
beta-methylcholine	affects expression	1
tamibarotene	affects expression	1
di-n-butylphosphoric acid	affects expression	1
bisphenol B	increases expression	1
abrine	decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	increases expression	1
pentabrominated diphenyl ether 100	decreases expression	1
bisphenol S	increases expression	1
jinfukang	affects cotreatment, increases expression	1
picoxystrobin	decreases expression	1
NSC 689534	affects binding, decreases expression	1
bisphenol AF	increases expression	1
Fulvestrant	affects cotreatment, increases methylation	1
Acetaminophen	decreases expression	1
Arsenic	decreases expression, increases abundance	1
Benztropine	affects cotreatment, decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652731	Binding	Binding affinity to human TWF2 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

3 cell lines: 2 cancer cell line, 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3KC	Abcam HEK293T TWF2 KO	Transformed cell line	Female
CVCL_TV26	HAP1 TWF2 (-) 1	Cancer cell line	Male
CVCL_TV27	HAP1 TWF2 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.