UBE2L5

Summary

UBE2L5 (ubiquitin conjugating enzyme E2 L5, HGNC:13477) is a protein-coding gene on chromosome 13q12.3, encoding Ubiquitin-conjugating enzyme E2 L5 (A0A1B0GUS4). Catalyzes the covalent attachment of ubiquitin to other proteins.

Predicted to enable ubiquitin conjugating enzyme activity. Predicted to be involved in protein K11-linked ubiquitination and ubiquitin-dependent protein catabolic process. Predicted to be active in nucleus.

Source: NCBI Gene 171222 — RefSeq curated summary.

At a glance

• MANE Select transcript: NM_001355247

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:13477
Approved symbol	UBE2L5
Name	ubiquitin conjugating enzyme E2 L5
Location	13q12.3
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000236444
Ensembl biotype	protein_coding
Entrez	171222

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 3 protein_coding

ENST00000436446, ENST00000635918, ENST00000638137

RefSeq mRNA: 1 — MANE Select: NM_001355247 NM_001355247

CCDS: CCDS86346

Canonical transcript exons

ENST00000635918 — 4 exons

Exon	Start	End
ENSE00003798135	30426725	30426778
ENSE00003798886	30427361	30429758
ENSE00003799724	30422493	30422677
ENSE00003800889	30424808	30424901

Expression profiles

Bgee: expression breadth ubiquitous, 115 present calls, max score 89.31.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.1682 / max 58.1497, expressed in 39 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
134612	0.1334	38
206992	0.0348	1

Top tissues by expression

126 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	89.31	gold quality
testis	UBERON:0000473	88.54	gold quality
right testis	UBERON:0004534	88.20	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	78.14	gold quality
right coronary artery	UBERON:0001625	60.04	gold quality
stromal cell of endometrium	CL:0002255	57.83	gold quality
left uterine tube	UBERON:0001303	56.66	gold quality
smooth muscle tissue	UBERON:0001135	56.14	gold quality
cortical plate	UBERON:0005343	55.29	silver quality
tibial artery	UBERON:0007610	55.08	gold quality
popliteal artery	UBERON:0002250	55.05	gold quality
body of uterus	UBERON:0009853	54.91	gold quality
calcaneal tendon	UBERON:0003701	54.34	gold quality
descending thoracic aorta	UBERON:0002345	53.50	gold quality
prefrontal cortex	UBERON:0000451	52.78	gold quality
myometrium	UBERON:0001296	52.30	gold quality
thoracic aorta	UBERON:0001515	52.21	gold quality
superior frontal gyrus	UBERON:0002661	52.21	gold quality
ascending aorta	UBERON:0001496	52.09	gold quality
ectocervix	UBERON:0012249	51.97	gold quality
endocervix	UBERON:0000458	51.67	gold quality
uterine cervix	UBERON:0000002	51.63	gold quality
endometrium	UBERON:0001295	51.40	gold quality
frontal cortex	UBERON:0001870	50.74	gold quality
Brodmann (1909) area 9	UBERON:0013540	50.71	gold quality
left coronary artery	UBERON:0001626	50.61	gold quality
lymph node	UBERON:0000029	50.44	gold quality
muscle tissue	UBERON:0002385	49.26	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	48.89	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	48.87	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	4.19

Regulation

Is transcription factor: no

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	ube2l3b	ENSDARG00000027141
danio_rerio	ube2l3a	ENSDARG00000112087
mus_musculus	Ube2l3	ENSMUSG00000038965
rattus_norvegicus	Ube2l3	ENSRNOG00000001862

Paralogs (12): UBE2D1 (ENSG00000072401), UBE2D4 (ENSG00000078967), UBE2D3 (ENSG00000109332), UBE2D2 (ENSG00000131508), UBE2Q2 (ENSG00000140367), UBE2L6 (ENSG00000156587), UBE2Q1 (ENSG00000160714), UBE2E3 (ENSG00000170035), UBE2E1 (ENSG00000170142), UBE2E2 (ENSG00000182247), UBE2L3 (ENSG00000185651), UBE2QL1 (ENSG00000215218)

Protein

Protein identifiers

Ubiquitin-conjugating enzyme E2 L5 — A0A1B0GUS4 (reviewed: A0A1B0GUS4)

Alternative names: Ubiquitin-protein ligase L5

All UniProt accessions (1): A0A1B0GUS4

UniProt curated annotations — full annotation on UniProt →

Function. Catalyzes the covalent attachment of ubiquitin to other proteins.

Pathway. Protein modification; protein ubiquitination.

Similarity. Belongs to the ubiquitin-conjugating enzyme family.

RefSeq proteins (1): NP_001342176* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000608	UBC	Domain
IPR016135	UBQ-conjugating_enzyme/RWD	Homologous_superfamily
IPR023313	UBQ-conjugating_AS	Active_site
IPR050113	Ub_conjugating_enzyme-E2-like	Family

Pfam: PF00179

UniProt features (3 total): chain 1, domain 1, active site 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A0A1B0GUS4-F1	93.77	0.91

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 86 (glycyl thioester intermediate)

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 21 (showing top): GOBP_MACROMOLECULE_CATABOLIC_PROCESS, GOBP_PROTEIN_K11_LINKED_UBIQUITINATION, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, GOBP_PROTEIN_POLYUBIQUITINATION, GOBP_PROTEIN_CATABOLIC_PROCESS, GOBP_PROTEOLYSIS, GOMF_UBIQUITIN_LIKE_PROTEIN_CONJUGATING_ENZYME_ACTIVITY, GOMF_ACYLTRANSFERASE_ACTIVITY, GOMF_AMINOACYLTRANSFERASE_ACTIVITY, GOMF_ADENYL_NUCLEOTIDE_BINDING, CHAF1B_TARGET_GENES, SFMBT1_TARGET_GENES, ZNF92_TARGET_GENES, GOBP_PROTEIN_MODIFICATION_BY_SMALL_PROTEIN_CONJUGATION, NFXL1_TARGET_GENES

GO Biological Process (4): ubiquitin-dependent protein catabolic process (GO:0006511), protein K11-linked ubiquitination (GO:0070979), protein ubiquitination (GO:0016567), protein modification by small protein conjugation (GO:0032446)

GO Molecular Function (6): ATP binding (GO:0005524), ligase activity (GO:0016874), ubiquitin conjugating enzyme activity (GO:0061631), nucleotide binding (GO:0000166), transferase activity (GO:0016740), ubiquitin-like protein transferase activity (GO:0019787)

GO Cellular Component (1): nucleus (GO:0005634)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
catalytic activity	2
protein ubiquitination	1
modification-dependent protein catabolic process	1
protein polyubiquitination	1
protein modification by small protein conjugation	1
protein modification by small protein conjugation or removal	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
ubiquitin-protein transferase activity	1
ubiquitin-like protein conjugating enzyme activity	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
aminoacyltransferase activity	1
catalytic activity, acting on a protein	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

1337 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
UBE2L5	RNF214	Q8ND24	525
UBE2L5	TATDN2	Q93075	480
UBE2L5	TM2D1	Q9BX74	416
UBE2L5	OAZ2	O95190	400
UBE2L5	TMCC3	Q9ULS5	379
UBE2L5	OST4	P0C6T2	369
UBE2L5	ARIH2	O95376	367
UBE2L5	SLITRK2	Q9H156	363
UBE2L5	USP48	Q86UV5	355
UBE2L5	GPRIN3	Q6ZVF9	351
UBE2L5	INTS10	Q9NVR2	350
UBE2L5	ZNF579	Q8NAF0	336
UBE2L5	NFATC2IP	Q8NCF5	335
UBE2L5	TCF19	Q9Y242	330
UBE2L5	RNF6	Q9Y252	330

IntAct

0 interactions, top by confidence:

ESM2 similar proteins: A0A1B0GUS4, A5PJC4, A5PKP9, D3ZDK2, O13685, O14933, O74196, O74549, P15731, P15732, P25867, P35129, P35131, P35132, P35133, P35134, P35135, P43102, P51668, P52487, P60604, P60605, P61080, P62837, P62838, P62839, P62840, P68036, P68037, P70711, Q17QG5, Q1RMX2, Q21633, Q2TA10, Q3MHP1, Q3ZCF7, Q4V8J2, Q5R5I4, Q5RF84, Q6C9W0

Diamond homologs: A0A1B0GUS4, A5PJC4, A5PKP9, D3ZDK2, O13685, O14933, O74196, O74810, P0C8G3, P0C8G4, P0C8G5, P15731, P15732, P21734, P25867, P25869, P27949, P35128, P35129, P35131, P35132, P35133, P35134, P35135, P43102, P46595, P49427, P51668, P51965, P52482, P52483, P52485, P52487, P52490, P52492, P61077, P61078, P61079, P61080, P61088

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
“Ub:E1 (UBA1 substrate)”	“up-regulates activity”	UBE2L5	ubiquitination
“Ub:E1 (UBA6 substrate)”	“up-regulates activity”	UBE2L5	ubiquitination

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

1030 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
13:30428275:G:C	W95C	0.995
13:30428275:G:T	W95C	0.995
13:30428273:T:A	W95R	0.994
13:30428273:T:C	W95R	0.994
13:30428396:T:C	F136L	0.993
13:30428398:C:A	F136L	0.993
13:30428398:C:G	F136L	0.993
13:30428141:T:C	F51L	0.992
13:30428143:C:A	F51L	0.992
13:30428143:C:G	F51L	0.992
13:30428007:G:T	R6M	0.991
13:30428093:T:A	W35R	0.991
13:30428093:T:C	W35R	0.991
13:30428218:C:A	H76Q	0.988
13:30428218:C:G	H76Q	0.988
13:30428217:A:G	H76R	0.987
13:30428008:G:C	R6S	0.986
13:30428008:G:T	R6S	0.986
13:30428142:T:C	F51S	0.986
13:30428216:C:G	H76D	0.986
13:30428274:G:C	W95S	0.985
13:30428177:T:C	F63L	0.984
13:30428179:C:A	F63L	0.984
13:30428179:C:G	F63L	0.984
13:30428313:T:C	L108P	0.983
13:30428409:C:A	A140D	0.982
13:30428274:G:T	W95L	0.981
13:30428018:G:A	E10K	0.979
13:30428205:C:T	T72I	0.979
13:30428007:G:C	R6T	0.978

dbSNP variants (sampled 300 via entrez): RS1000305236 (13:30427758 G>C), RS1000447112 (13:30420536 G>T), RS1001095716 (13:30427071 T>C), RS1001223022 (13:30421592 A>G), RS1001393913 (13:30427849 A>T), RS1001423614 (13:30428124 C>G,T), RS1001428139 (13:30428637 G>A), RS1001652237 (13:30421351 T>C), RS1001715270 (13:30421809 C>A,G,T), RS1002222825 (13:30421926 C>G,T), RS1002427871 (13:30426501 A>G), RS1002985843 (13:30423899 C>T), RS1003101521 (13:30429731 A>G), RS1003134137 (13:30429942 C>T), RS1003235275 (13:30425158 C>A,G)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.