USP24

Summary

USP24 (ubiquitin specific peptidase 24, HGNC:12623) is a protein-coding gene on chromosome 1p32.3, encoding Ubiquitin carboxyl-terminal hydrolase 24 (Q9UPU5). Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53.

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP24 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).

Source: NCBI Gene 23358 — RefSeq curated summary.

At a glance

• GWAS associations: 7
• Clinical variants (ClinVar): 291 total — 1 likely-pathogenic
• Druggable target: yes
• MANE Select transcript: NM_015306

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:12623
Approved symbol	USP24
Name	ubiquitin specific peptidase 24
Location	1p32.3
Locus type	gene with protein product
Status	Approved
Aliases	KIAA1057
Ensembl gene	ENSG00000162402
Ensembl biotype	protein_coding
OMIM	610569
Entrez	23358

Gene structure

Transcript identifiers

Ensembl transcripts: 9 — 4 protein_coding, 3 retained_intron, 1 protein_coding_CDS_not_defined, 1 nonsense_mediated_decay

ENST00000294383, ENST00000472566, ENST00000480962, ENST00000482197, ENST00000484447, ENST00000512504, ENST00000634589, ENST00000927917, ENST00000927918

RefSeq mRNA: 1 — MANE Select: NM_015306 NM_015306

CCDS: CCDS44154

Canonical transcript exons

ENST00000294383 — 68 exons

Exon	Start	End
ENSE00001064819	55077235	55077300
ENSE00001064828	55093937	55094087
ENSE00001139439	55075457	55075523
ENSE00001139457	55078538	55078651
ENSE00001139465	55079538	55079659
ENSE00001139476	55081322	55081424
ENSE00001139504	55085942	55086038
ENSE00001139510	55089627	55089740
ENSE00001139516	55092023	55092126
ENSE00001139522	55092821	55092916
ENSE00001139535	55095255	55095396
ENSE00001139544	55096498	55096622
ENSE00001139555	55096952	55097172
ENSE00001139565	55097598	55097717
ENSE00001139572	55097943	55098084
ENSE00001139580	55098476	55098558
ENSE00001139585	55099771	55099869
ENSE00001139590	55100839	55100964
ENSE00001139599	55101584	55101703
ENSE00001156674	55103876	55104020
ENSE00001370828	55214790	55215364
ENSE00001382948	55177967	55178132
ENSE00001415442	55106146	55106263
ENSE00001433950	55107239	55107430
ENSE00001434462	55110185	55110246
ENSE00001454764	55120596	55120756
ENSE00001454765	55121436	55121506
ENSE00001454766	55123447	55123602
ENSE00001454767	55124469	55124628
ENSE00001454768	55125320	55125548
ENSE00001454769	55125663	55125758
ENSE00001454770	55129477	55129574
ENSE00001454771	55132545	55132700
ENSE00001454772	55134070	55134163
ENSE00001454773	55134328	55134413
ENSE00001454774	55137515	55137688
ENSE00001454775	55137806	55137904
ENSE00001454776	55138608	55138718
ENSE00001454777	55138944	55139010
ENSE00001454778	55141616	55141731
ENSE00001454779	55142742	55142795
ENSE00001454780	55142979	55143119
ENSE00001454781	55144127	55144203
ENSE00001454786	55153870	55153917
ENSE00001454788	55154371	55154466
ENSE00001601173	55162199	55162264
ENSE00001613119	55176376	55176443
ENSE00001623489	55159611	55159685
ENSE00001629856	55148463	55148570
ENSE00001639761	55146929	55147060
ENSE00001659875	55172377	55172520
ENSE00001687516	55158878	55159036
ENSE00001701698	55154119	55154280
ENSE00001706381	55171556	55171678
ENSE00001728065	55147649	55147798
ENSE00001744360	55157256	55157370
ENSE00001747335	55165885	55165950
ENSE00001756617	55166568	55166603
ENSE00001780813	55156948	55157051
ENSE00001803592	55145998	55146109
ENSE00001805718	55154671	55154778
ENSE00001847641	55066359	55069107
ENSE00003481075	55072786	55072861
ENSE00003529998	55071814	55071924
ENSE00003571309	55083272	55083364
ENSE00003575373	55072317	55072403
ENSE00003648271	55083772	55083888
ENSE00003681127	55073828	55073906

Expression profiles

Bgee: expression breadth ubiquitous, 294 present calls, max score 95.80.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 13.1843 / max 265.8441, expressed in 1779 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
12491	12.3430	1774
12492	0.8413	383

Top tissues by expression

298 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	95.80	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	94.96	gold quality
biceps brachii	UBERON:0001507	94.84	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	94.71	gold quality
gastrocnemius	UBERON:0001388	94.66	gold quality
muscle of leg	UBERON:0001383	94.37	gold quality
hindlimb stylopod muscle	UBERON:0004252	94.03	gold quality
subcutaneous adipose tissue	UBERON:0002190	93.92	gold quality
cerebellar hemisphere	UBERON:0002245	93.82	gold quality
right hemisphere of cerebellum	UBERON:0014890	93.77	gold quality
calcaneal tendon	UBERON:0003701	93.76	gold quality
cerebellar cortex	UBERON:0002129	93.72	gold quality
colonic epithelium	UBERON:0000397	93.54	gold quality
cerebellum	UBERON:0002037	93.26	gold quality
tibial nerve	UBERON:0001323	93.05	gold quality
muscle organ	UBERON:0001630	92.94	gold quality
skeletal muscle organ	UBERON:0014892	92.94	gold quality
skin of leg	UBERON:0001511	92.68	gold quality
skin of abdomen	UBERON:0001416	92.46	gold quality
granulocyte	CL:0000094	92.42	gold quality
mucosa of stomach	UBERON:0001199	92.33	gold quality
ventricular zone	UBERON:0003053	92.17	gold quality
adipose tissue	UBERON:0001013	92.14	gold quality
right lung	UBERON:0002167	92.03	gold quality
adrenal tissue	UBERON:0018303	92.00	gold quality
connective tissue	UBERON:0002384	91.95	gold quality
left ovary	UBERON:0002119	91.54	gold quality
zone of skin	UBERON:0000014	91.08	gold quality
body of uterus	UBERON:0009853	90.92	gold quality
right ovary	UBERON:0002118	90.78	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-CURD-119	yes	50.98
E-ANND-3	yes	10.14

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): NFKB

miRNA regulators (miRDB)

204 targeting USP24, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-340-5P	100.00	72.50	4437
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-4776-3P	100.00	68.73	1340
HSA-MIR-3064-3P	100.00	70.09	1254
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-4500	99.99	72.72	2367
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-34A-5P	99.99	71.21	1784
HSA-MIR-449A	99.99	71.05	1776
HSA-MIR-12136	99.98	72.81	5713
HSA-LET-7A-5P	99.98	72.29	1790
HSA-LET-7B-5P	99.98	72.31	1790
HSA-LET-7C-5P	99.98	72.29	1790
HSA-LET-7E-5P	99.98	72.29	1790
HSA-LET-7F-5P	99.98	72.56	1784
HSA-LET-7G-5P	99.98	72.37	1784
HSA-LET-7I-5P	99.98	72.37	1788
HSA-MIR-98-5P	99.98	72.33	1787
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-499A-5P	99.98	70.79	1323
HSA-MIR-103A-3P	99.98	69.14	1595
HSA-MIR-107	99.98	69.14	1595
HSA-MIR-548AN	99.97	70.91	2817

Literature-anchored findings (GeneRIF, showing 16)

• Data suggest that genetic variants in USP24 affect the risk for late-onset Parkinson disease (PD), which is consistent with the predicted role of the ubiquitination pathway in PD etiology. (PMID:16917932)
• USP24 alone plays a role in PD susceptibility among Taiwanese people >or=60 years of age, or acting synergistically with USP40 and UCHL1 in the total subjects. (PMID:20302855)
• The present study is the first to report a lack of association between SNPs of USP24 and parkinson disease in Han Chinese patients. Other (PMID:22923019)
• The knockdown of USP24 in two human cell lines decreased the steady-state levels of DDB2, indicating that USP24-mediated DDB2 deubiquitination prevents DDB2 degradation. (PMID:23159851)
• These results suggested that USP24 expression is tightly regulated at its transcription level and NFkappaB plays an important role in this process. (PMID:24286619)
• USP24 deubiquitinase regulates the DNA damage response by directly targeting the p53 tumor suppressor. (PMID:25578727)
• the novel compound EOAI3402143 dose-dependently inhibited Usp9x and Usp24 activity, increased tumor cell apoptosis, and fully blocked or regressed myeloma tumors in mice. (PMID:25814533)
• Data show that ubiquitin-specific peptidase 24 (USP24) is highly expressed in cell lines with enhanced malignancy and in late-stage lung cancer clinical samples. (PMID:26568301)
• USP24 level was decreased during the early stage of cancer and the mitotic stage of the cell cycle to regulate its substrates p300, Bax, E2F4 and securin, resulting in decreased cell apoptosis and increased cell cycle progression and, thus, cancer formation. (PMID:27991932)
• Data indicate that ubiquitin specific peptidase 24 (USP24) promotes Interleukin-6 (IL-6) expression, which might be beneficial for cancer therapy. (PMID:30266897)
• The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability. (PMID:30957634)
• USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy. (PMID:33257797)
• USP24 Is a Cancer-Associated Ubiquitin Hydrolase, Novel Tumor Suppressor, and Chromosome Instability Gene Deleted in Neuroblastoma. (PMID:33355202)
• USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway. (PMID:34326684)
• MiR-21-5p promotes sorafenib resistance and hepatocellular carcinoma progression by regulating SIRT7 ubiquitination through USP24. (PMID:37187452)
• USP24 promotes hepatocellular carcinoma tumorigenesis through deubiquitinating and stabilizing TRAF2. (PMID:39127151)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Usp24	ENSMUSG00000028514
rattus_norvegicus	Usp24	ENSRNOG00000005802

Paralogs (71): USP2 (ENSG00000036672), USP28 (ENSG00000048028), USP36 (ENSG00000055483), USP13 (ENSG00000058056), USP33 (ENSG00000077254), USP40 (ENSG00000085982), USP48 (ENSG00000090686), USP14 (ENSG00000101557), USP11 (ENSG00000102226), USP10 (ENSG00000103194), USP31 (ENSG00000103404), USP42 (ENSG00000106346), USP5 (ENSG00000111667), USP4 (ENSG00000114316), USP9Y (ENSG00000114374), USP34 (ENSG00000115464), USP35 (ENSG00000118369), USP45 (ENSG00000123552), USP22 (ENSG00000124422), USP9X (ENSG00000124486), USP6 (ENSG00000129204), USP29 (ENSG00000131864), USP26 (ENSG00000134588), USP30 (ENSG00000135093), USP15 (ENSG00000135655), USP37 (ENSG00000135913), USP44 (ENSG00000136014), USP20 (ENSG00000136878), USP8 (ENSG00000138592), USP3 (ENSG00000140455), USP21 (ENSG00000143258), USP43 (ENSG00000154914), USP25 (ENSG00000155313), USP16 (ENSG00000156256), USP1 (ENSG00000162607), USP49 (ENSG00000164663), USP38 (ENSG00000170185), USP50 (ENSG00000170236), USP47 (ENSG00000170242), USP32 (ENSG00000170832)

Protein

Protein identifiers

Ubiquitin carboxyl-terminal hydrolase 24 — Q9UPU5 (reviewed: Q9UPU5)

Alternative names: Deubiquitinating enzyme 24, Ubiquitin thioesterase 24, Ubiquitin-specific-processing protease 24

All UniProt accessions (3): A0A0U1RQI9, A0A0U1RRH2, Q9UPU5

UniProt curated annotations — full annotation on UniProt →

Function. Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron.

Subunit / interactions. (Microbial infection) Interacts with human cytomegalovirus protein UL38.

Similarity. Belongs to the peptidase C19 family.

RefSeq proteins (1): NP_056121* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001394	Peptidase_C19_UCH	Domain
IPR009060	UBA-like_sf	Homologous_superfamily
IPR015940	UBA	Domain
IPR016024	ARM-type_fold	Homologous_superfamily
IPR018200	USP_CS	Conserved_site
IPR028889	USP	Domain
IPR029071	Ubiquitin-like_domsf	Homologous_superfamily
IPR033382	USP24_UBA	Domain
IPR038765	Papain-like_cys_pep_sf	Homologous_superfamily
IPR047061	UBP24_Ubl	Domain
IPR050164	Peptidase_C19	Family
IPR055176	UBP24/USP9X/USP9Y_UBL	Domain
IPR056850	ARM_UBP34_24_USP9X_Y	Domain

Pfam: PF00443, PF22900, PF25010

UniProt features (36 total): modified residue 11, region of interest 6, compositionally biased region 5, sequence conflict 5, sequence variant 4, domain 2, active site 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9UPU5-F1	76.15	0.19

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (2): 1698 (nucleophile); 1970 (proton acceptor)

Post-translational modifications (11): 63, 88, 942, 1141, 1285, 1943, 2047, 2077, 2561, 2565, 2604

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-5689880	Ub-specific processing proteases

MSigDB gene sets: 198 (showing top): GSE18804_SPLEEN_MACROPHAGE_VS_TUMORAL_MACROPHAGE_UP, IVANOVA_HEMATOPOIESIS_LATE_PROGENITOR, GOBP_PROTEIN_MODIFICATION_BY_SMALL_PROTEIN_REMOVAL, MILI_PSEUDOPODIA_HAPTOTAXIS_UP, GOMF_CYSTEINE_TYPE_PEPTIDASE_ACTIVITY, DACOSTA_UV_RESPONSE_VIA_ERCC3_COMMON_DN, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, GOBP_REGULATION_OF_PROTEIN_STABILITY, GOBP_PROTEOLYSIS, WILSON_PROTEASES_AT_TUMOR_BONE_INTERFACE_UP, DAZARD_UV_RESPONSE_CLUSTER_G6, GOMF_PEPTIDASE_ACTIVITY, GOMF_UBIQUITIN_LIKE_PROTEIN_PEPTIDASE_ACTIVITY, REACTOME_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, MORF_CDC16

GO Biological Process (3): proteolysis (GO:0006508), protein deubiquitination (GO:0016579), regulation of protein stability (GO:0031647)

GO Molecular Function (5): cysteine-type deubiquitinase activity (GO:0004843), protein binding (GO:0005515), peptidase activity (GO:0008233), cysteine-type peptidase activity (GO:0008234), hydrolase activity (GO:0016787)

GO Cellular Component (3): nucleus (GO:0005634), nucleoplasm (GO:0005654), cytosol (GO:0005829)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Deubiquitination	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
protein metabolic process	1
cysteine-type deubiquitinase activity	1
protein modification by small protein removal	1
regulation of biological quality	1
cysteine-type peptidase activity	1
deubiquitinase activity	1
binding	1
hydrolase activity	1
catalytic activity, acting on a protein	1
peptidase activity	1
catalytic activity	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cytoplasm	1

Protein interactions and networks

STRING

1234 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
USP24	CDCP2	Q5VXM1	890
USP24	DDB2	Q92466	820
USP24	HIVEP3	Q5T1R4	799
USP24	ELAVL4	P26378	790
USP24	EIF2B3	Q9NR50	773
USP24	USP13	Q92995	667
USP24	ZUP1	Q96AP4	590
USP24	USP5	P45974	587
USP24	USP14	P54578	545
USP24	OTUB1	Q96FW1	481
USP24	BSND	Q8WZ55	473
USP24	OTUD5	Q96G74	462
USP24	DDB1	Q16531	449
USP24	USP1	O94782	446
USP24	UCHL3	P15374	428

IntAct

115 interactions, top by confidence:

A	B	Type	Score
CNOT2	CNOT1	psi-mi:“MI:0914”(association)	0.740
HIF1AN	GMDS	psi-mi:“MI:0914”(association)	0.640
USP24		psi-mi:“MI:0915”(physical association)	0.560
HTT	USP24	psi-mi:“MI:0915”(physical association)	0.560
NCS1	NMT2	psi-mi:“MI:0914”(association)	0.530
SUV39H1	MAGEC1	psi-mi:“MI:0914”(association)	0.530

BioGRID (153): USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Co-fractionation), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS), TP53 (Biochemical Activity), USP24 (Affinity Capture-MS), USP24 (Affinity Capture-MS)

ESM2 similar proteins: A0JNG7, A0JPF5, A0JPG1, A2VE70, B0V207, B1AY13, B4F766, F1QFR9, F1R2X6, O17482, O43156, P49021, P50851, Q05DH4, Q0P4Q0, Q15021, Q4S6U8, Q505K2, Q5PNP1, Q5RAW5, Q5SP90, Q5SSW2, Q5W0V3, Q5ZLW3, Q6DCP6, Q6IN85, Q6INN7, Q6NRP2, Q6P2K6, Q7RTS9, Q80TR8, Q80YR2, Q86V87, Q8CDM8, Q8CHY3, Q8IV36, Q8IY22, Q8K2Z4, Q8NFP9, Q8R1F6

Diamond homologs: A0A0R4IB93, A0JM59, A5PMR2, A5PN09, A6NNY8, A6QNM7, A7Z056, B1AY13, B1WBD7, D2HBJ8, D6RBM5, E1C213, E7F6T8, E9Q9U0, F6Z5C0, F8VPU6, F8VPZ3, M9PD06, O00507, O22207, O60079, O74442, O94269, O94966, O96612, P0C7I0, P0C8Z3, P0CAQ1, P35125, P39538, P40453, P51784, P53874, P55824, P70398, Q01988, Q09738, Q0V9G5, Q28CN3, Q2HJE4

SIGNOR signaling

10 interactions.

A	Effect	B	Mechanism
USP24	up-regulates	DDB2	deubiquitination
CDK1	“down-regulates quantity by destabilization”	USP24	phosphorylation
USP24	“up-regulates quantity by stabilization”	BAX	deubiquitination
USP24	“up-regulates quantity by stabilization”	EP300	deubiquitination
CyclinB/CDK1	“down-regulates quantity by destabilization”	USP24	phosphorylation
ATM	“up-regulates activity”	USP24	phosphorylation

Disease & clinical

Clinical variants and AI predictions

ClinVar

291 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	1
Uncertain significance	218
Likely benign	5
Benign	5

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
599595	NM_015306.3(USP24):c.7448-7C>T	Likely pathogenic

SpliceAI

9058 predictions. Top by Δscore:

Variant	Effect	Δscore
1:55069103:GAACC:G	acceptor_gain	1.0000
1:55069104:AACC:A	acceptor_gain	1.0000
1:55069105:ACC:A	acceptor_gain	1.0000
1:55069106:CC:C	acceptor_gain	1.0000
1:55069106:CCC:C	acceptor_gain	1.0000
1:55069107:CC:C	acceptor_gain	1.0000
1:55069108:C:CC	acceptor_gain	1.0000
1:55069108:C:T	acceptor_gain	1.0000
1:55071808:TTATA:T	donor_loss	1.0000
1:55071809:TATA:T	donor_loss	1.0000
1:55071810:ATAC:A	donor_loss	1.0000
1:55071812:ACCTG:A	donor_loss	1.0000
1:55071813:CCTG:C	donor_gain	1.0000
1:55071813:CCTGC:C	donor_loss	1.0000
1:55071923:TCCTG:T	acceptor_loss	1.0000
1:55071925:CTGC:C	acceptor_loss	1.0000
1:55071926:T:A	acceptor_loss	1.0000
1:55072311:TCTCA:T	donor_loss	1.0000
1:55072312:CTCA:C	donor_loss	1.0000
1:55072313:TCAC:T	donor_loss	1.0000
1:55072314:CAC:C	donor_loss	1.0000
1:55072315:A:C	donor_loss	1.0000
1:55072316:C:A	donor_loss	1.0000
1:55072401:CAT:C	acceptor_gain	1.0000
1:55072403:TC:T	acceptor_loss	1.0000
1:55072404:C:CC	acceptor_gain	1.0000
1:55072404:CT:C	acceptor_loss	1.0000
1:55072781:GTTA:G	donor_loss	1.0000
1:55072782:TTA:T	donor_loss	1.0000
1:55072783:TA:T	donor_loss	1.0000

AlphaMissense

17204 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:55071903:C:G	A2571P	1.000
1:55071908:G:T	A2569D	1.000
1:55071909:C:G	A2569P	1.000
1:55072321:G:T	A2562D	1.000
1:55072330:G:A	T2559I	1.000
1:55072338:A:C	F2556L	1.000
1:55072338:A:T	F2556L	1.000
1:55072339:A:C	F2556C	1.000
1:55072339:A:G	F2556S	1.000
1:55072340:A:G	F2556L	1.000
1:55072796:A:G	L2531P	1.000
1:55072800:A:G	W2530R	1.000
1:55072800:A:T	W2530R	1.000
1:55072818:A:G	W2524R	1.000
1:55072818:A:T	W2524R	1.000
1:55073851:T:A	K2501N	1.000
1:55073851:T:G	K2501N	1.000
1:55073853:T:C	K2501E	1.000
1:55073865:A:C	Y2497D	1.000
1:55081415:A:G	W2329R	1.000
1:55081415:A:T	W2329R	1.000
1:55089724:A:G	W2191R	1.000
1:55089724:A:T	W2191R	1.000
1:55095343:A:C	Y2039D	1.000
1:55095348:A:G	L2037P	1.000
1:55095348:A:T	L2037H	1.000
1:55095357:G:T	A2034D	1.000
1:55095364:A:G	W2032R	1.000
1:55095364:A:T	W2032R	1.000
1:55096530:C:T	G2010E	1.000

dbSNP variants (sampled 300 via entrez): RS1000016326 (1:55206996 A>C,G), RS1000021167 (1:55180421 A>T), RS1000049358 (1:55072294 C>G,T), RS1000071080 (1:55143885 CAG>C), RS1000104225 (1:55157662 G>A,T), RS1000143625 (1:55131233 T>A), RS1000146437 (1:55066074 C>A,T), RS1000167037 (1:55118086 T>G), RS1000190687 (1:55100032 C>T), RS1000200995 (1:55078481 C>A), RS1000220346 (1:55162782 G>A), RS1000247025 (1:55204160 T>C), RS1000260823 (1:55085059 G>T), RS1000292267 (1:55182297 C>T), RS1000433644 (1:55098968 T>C)

Disease associations

OMIM: gene MIM:610569 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

7 associations (top):

Study	Trait	p-value
GCST001639_11	Metabolite levels	5.000000e-13
GCST002724_11	Airway responsiveness in chronic obstructive pulmonary disease	9.000000e-08
GCST002724_7	Airway responsiveness in chronic obstructive pulmonary disease	5.000000e-07
GCST004136_4	Methadone dose in opioid dependence	2.000000e-06
GCST008159_82	Waist-to-hip ratio adjusted for BMI	2.000000e-06
GCST011924_3	LDL levels x fish oil supplementation interaction (2df)	3.000000e-31
GCST011926_1	Total cholesterol levels x fish oil supplementation interaction (2df)	2.000000e-20

EFO canonical traits (7, from GWAS)

EFO ID	Trait name
EFO:0004723	coronary artery calcification
EFO:0006897	airway responsiveness measurement
EFO:0007907	methadone dose measurement
EFO:0007788	BMI-adjusted waist-hip ratio
EFO:0004611	low density lipoprotein cholesterol measurement
EFO:0600007	fish oil supplement exposure measurement
EFO:0004574	total cholesterol measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL5291599 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

PharmGKB variants

1 variants.

Variant	Genes	Level	Score	#Clin annots	Drugs
rs17111584	USP24		0.00	0

ChEMBL bioactivities

1 potent at pChembl≥5 of 3 total, top 1 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.60	IC50	2500	nM	CHEMBL4287357

PubChem BioAssay actives

1 with measured affinity, of 9 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
(E)-2-cyano-3-(3,6-dichloro-2-pyridinyl)-N-[1-[4-(2-morpholin-4-ylethoxy)phenyl]butyl]prop-2-enamide	1930830: Inhibition of USP24 (unknown origin)	ic50	2.5000	uM

CTD chemical–gene interactions

48 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects cotreatment, affects expression, increases abundance, decreases expression, decreases methylation (+1 more)	5
Valproic Acid	affects expression, decreases expression	3
bisphenol F	increases expression, affects cotreatment, decreases expression	2
trichostatin A	affects expression, increases expression	2
sodium arsenite	affects cotreatment, increases abundance, decreases expression	2
Arsenic	increases ubiquitination, affects cotreatment, decreases expression, increases abundance	2
Cisplatin	decreases expression	2
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide	decreases expression	2
aristolochic acid I	decreases expression	1
FR900359	affects phosphorylation	1
ginger extract	affects cotreatment, affects expression, increases abundance	1
2,4,6-tribromophenol	decreases expression	1
triphenyl phosphate	affects expression	1
decabromobiphenyl ether	decreases expression	1
butyraldehyde	decreases expression	1
manganese chloride	affects cotreatment, decreases expression, increases abundance	1
potassium chromate(VI)	affects cotreatment, decreases expression	1
N,N,N’,N’-tetrakis(2-pyridylmethyl)ethylenediamine	decreases expression	1
beta-methylcholine	affects expression	1
epigallocatechin gallate	affects cotreatment, decreases expression	1
CGP 52608	affects binding, increases reaction	1
bisphenol B	increases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
hexabrominated diphenyl ether 153	decreases expression	1
bisphenol S	increases expression	1
jinfukang	decreases expression	1
bisphenol AF	increases expression	1
Irinotecan	decreases expression	1
Resveratrol	affects cotreatment, increases expression	1
Sunitinib	increases expression	1

ChEMBL screening assays

8 unique, capped per target: 8 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5234106	Binding	Inhibition of USP24 (unknown origin)	Recent advances in the development of ubiquitin-specific-processing protease 7 (USP7) inhibitors. — Eur J Med Chem

Cellosaurus cell lines

2 cell lines: 1 transformed cell line, 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B3L0	Abcam HEK293T USP24 KO	Transformed cell line	Female
CVCL_TW78	HAP1 USP24 (-)	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.