USP32

Summary

USP32 (ubiquitin specific peptidase 32, HGNC:19143) is a protein-coding gene on chromosome 17q23.3, encoding Ubiquitin carboxyl-terminal hydrolase 32 (Q8NFA0). Deubiquitinase that can remove conjugated ubiquitin from target proteins, such as RAB7A and LAMTOR1.

Enables cysteine-type deubiquitinase activity. Involved in positive regulation of TORC1 signaling. Located in cytosol. Is active in Golgi apparatus.

Source: NCBI Gene 84669 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 186 total
• Druggable target: yes
• MANE Select transcript: NM_032582

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:19143
Approved symbol	USP32
Name	ubiquitin specific peptidase 32
Location	17q23.3
Locus type	gene with protein product
Status	Approved
Aliases	NY-REN-60, USP10
Ensembl gene	ENSG00000170832
Ensembl biotype	protein_coding
OMIM	607740
Entrez	84669

Gene structure

Transcript identifiers

Ensembl transcripts: 16 — 10 protein_coding, 4 retained_intron, 1 protein_coding_CDS_not_defined, 1 nonsense_mediated_decay

ENST00000300896, ENST00000393003, ENST00000585720, ENST00000586238, ENST00000586881, ENST00000587651, ENST00000588898, ENST00000589335, ENST00000589552, ENST00000589761, ENST00000590133, ENST00000590297, ENST00000591768, ENST00000592339, ENST00000593071, ENST00000923223

RefSeq mRNA: 1 — MANE Select: NM_032582 NM_032582

CCDS: CCDS32697

Canonical transcript exons

ENST00000300896 — 34 exons

Exon	Start	End
ENSE00001112928	60288523	60288682
ENSE00001112933	60271350	60271481
ENSE00001112935	60265976	60266091
ENSE00001112936	60269450	60269557
ENSE00001112938	60265412	60265474
ENSE00001112943	60181324	60181748
ENSE00001206983	60209370	60209543
ENSE00001231039	60252381	60252442
ENSE00001231200	60226039	60226231
ENSE00001305865	60236138	60236240
ENSE00002235197	60214620	60214774
ENSE00002260262	60219670	60219787
ENSE00002330307	60205447	60205658
ENSE00002349779	60192844	60192930
ENSE00002355844	60208654	60208828
ENSE00002370017	60208059	60208210
ENSE00002371009	60183165	60183453
ENSE00002373920	60180545	60180637
ENSE00002377614	60198260	60198444
ENSE00002381312	60212024	60212098
ENSE00002392397	60185460	60185651
ENSE00002392724	60190563	60190683
ENSE00002398386	60207021	60207132
ENSE00002403606	60211376	60211514
ENSE00002429622	60211013	60211118
ENSE00002823319	60391882	60392133
ENSE00002928473	60177327	60179428
ENSE00003550114	60345481	60345608
ENSE00003620914	60301599	60301704
ENSE00003646149	60223411	60223586
ENSE00003667080	60222409	60222549
ENSE00003759855	60255175	60255258
ENSE00003790606	60294683	60294801
ENSE00003790954	60213581	60213662

Expression profiles

Bgee: expression breadth ubiquitous, 280 present calls, max score 96.94.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 18.0948 / max 275.5939, expressed in 1800 samples.

FANTOM5 promoters (11 alternative TSS)

Promoter ID	TPM avg	Samples expressed
167375	9.0303	1706
167373	3.4854	1386
167372	2.5288	1198
167371	0.8777	437
167378	0.8535	310
167374	0.5667	271
167377	0.3188	161
167380	0.1932	75
167376	0.0936	25
167379	0.0778	26

Top tissues by expression

286 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	96.94	gold quality
right testis	UBERON:0004534	96.69	gold quality
sural nerve	UBERON:0015488	95.57	gold quality
testis	UBERON:0000473	94.90	gold quality
sperm	CL:0000019	93.73	gold quality
adrenal tissue	UBERON:0018303	93.40	gold quality
blood	UBERON:0000178	93.16	gold quality
monocyte	CL:0000576	92.20	gold quality
mononuclear cell	CL:0000842	92.00	gold quality
male germ cell	CL:0000015	91.90	gold quality
leukocyte	CL:0000738	91.73	gold quality
lower esophagus mucosa	UBERON:0035834	91.62	gold quality
calcaneal tendon	UBERON:0003701	91.08	gold quality
gastrocnemius	UBERON:0001388	90.36	gold quality
prefrontal cortex	UBERON:0000451	90.12	gold quality
muscle of leg	UBERON:0001383	89.79	gold quality
islet of Langerhans	UBERON:0000006	89.15	gold quality
tendon	UBERON:0000043	89.15	gold quality
medial globus pallidus	UBERON:0002477	88.88	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	88.83	gold quality
corpus callosum	UBERON:0002336	88.67	gold quality
cervix squamous epithelium	UBERON:0006922	88.59	silver quality
stromal cell of endometrium	CL:0002255	88.40	gold quality
cortical plate	UBERON:0005343	88.11	gold quality
trabecular bone tissue	UBERON:0002483	87.85	gold quality
bone marrow cell	CL:0002092	87.84	gold quality
right lung	UBERON:0002167	87.39	gold quality
bone marrow	UBERON:0002371	87.06	gold quality
colonic epithelium	UBERON:0000397	87.00	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.89	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	9.16

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

218 targeting USP32, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5692A	100.00	74.40	6850
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-6873-3P	100.00	71.42	2626
HSA-MIR-12118	100.00	65.88	1270
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-3924	100.00	72.09	2394
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-196A-1-3P	99.99	72.15	2772
HSA-MIR-4500	99.99	72.72	2367
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-33A-5P	99.99	68.62	1055
HSA-MIR-33B-5P	99.99	68.58	1062
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-3173-3P	99.98	66.49	1217
HSA-MIR-6891-5P	99.98	66.53	1372
HSA-LET-7A-5P	99.98	72.29	1790
HSA-LET-7B-5P	99.98	72.31	1790
HSA-LET-7C-5P	99.98	72.29	1790
HSA-LET-7E-5P	99.98	72.29	1790
HSA-LET-7F-5P	99.98	72.56	1784

Literature-anchored findings (GeneRIF, showing 11)

• USP32 is overexpressed in breast cancer. (PMID:20549504)
• USP32 silencing arrested cell cycle progression at G0/G1 phase via decreasing CDK4/Cyclin D1 complex and elevating p21. (PMID:28597490)
• the small GTPase Rab7-the logistical centerpiece of late endosome biology-is a substrate of USP32. (PMID:30926795)
• USP32 promotes tumorigenesis and chemoresistance in gastric carcinoma via upregulation of SMAD2. (PMID:32226309)
• Ubiquitin specific peptidase 32 acts as an oncogene in epithelial ovarian cancer by deubiquitylating farnesyl-diphosphate farnesyltransferase 1. (PMID:33744759)
• USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2. (PMID:34815782)
• Identification of ubiquitin-specific protease 32 as an oncogene in glioblastoma and the underlying mechanisms. (PMID:35440702)
• Deubiquitylation of Rab35 by USP32 promotes the transmission of imatinib resistance by enhancing exosome secretion in gastrointestinal stromal tumours. (PMID:36725886)
• Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells. (PMID:36951484)
• High USP32 expression contributes to cancer progression and is correlated with immune infiltrates in hepatocellular carcinoma. (PMID:37957631)
• Furin Egress from the TGN is Regulated by Membrane-Associated RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32 (USP32) via Nondegradable K33-Polyubiquitination. (PMID:39031635)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	usp32	ENSDARG00000061895
mus_musculus	Usp32	ENSMUSG00000000804
rattus_norvegicus	Usp32	ENSRNOG00000027711

Paralogs (71): USP2 (ENSG00000036672), USP28 (ENSG00000048028), USP36 (ENSG00000055483), USP13 (ENSG00000058056), USP33 (ENSG00000077254), USP40 (ENSG00000085982), USP48 (ENSG00000090686), USP14 (ENSG00000101557), USP11 (ENSG00000102226), USP10 (ENSG00000103194), USP31 (ENSG00000103404), USP42 (ENSG00000106346), USP5 (ENSG00000111667), USP4 (ENSG00000114316), USP9Y (ENSG00000114374), USP34 (ENSG00000115464), USP35 (ENSG00000118369), USP45 (ENSG00000123552), USP22 (ENSG00000124422), USP9X (ENSG00000124486), USP6 (ENSG00000129204), USP29 (ENSG00000131864), USP26 (ENSG00000134588), USP30 (ENSG00000135093), USP15 (ENSG00000135655), USP37 (ENSG00000135913), USP44 (ENSG00000136014), USP20 (ENSG00000136878), USP8 (ENSG00000138592), USP3 (ENSG00000140455), USP21 (ENSG00000143258), USP43 (ENSG00000154914), USP25 (ENSG00000155313), USP16 (ENSG00000156256), USP24 (ENSG00000162402), USP1 (ENSG00000162607), USP49 (ENSG00000164663), USP38 (ENSG00000170185), USP50 (ENSG00000170236), USP47 (ENSG00000170242)

Protein

Protein identifiers

Ubiquitin carboxyl-terminal hydrolase 32 — Q8NFA0 (reviewed: Q8NFA0)

Alternative names: Deubiquitinating enzyme 32, Renal carcinoma antigen NY-REN-60, Ubiquitin thioesterase 32, Ubiquitin-specific-processing protease 32

All UniProt accessions (9): Q8NFA0, K7EII4, K7EKK3, K7EKZ1, K7EL53, K7EN13, K7ENU6, K7EPL4, K7EQL6

UniProt curated annotations — full annotation on UniProt →

Function. Deubiquitinase that can remove conjugated ubiquitin from target proteins, such as RAB7A and LAMTOR1. Acts as a positive regulator of the mTORC1 signaling by mediating deubiquitination of LAMTOR1, thereby promoting the association between LAMTOR1 and the lysosomal V-ATPase complex and subsequent activation of the mTORC1 complex.

Subcellular location. Golgi apparatus membrane.

Similarity. Belongs to the peptidase C19 family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q8NFA0-1	1	yes
Q8NFA0-2	2

RefSeq proteins (1): NP_115971* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001394	Peptidase_C19_UCH	Domain
IPR002048	EF_hand_dom	Domain
IPR006615	Pept_C19_DUSP	Domain
IPR011992	EF-hand-dom_pair	Homologous_superfamily
IPR018200	USP_CS	Conserved_site
IPR018247	EF_Hand_1_Ca_BS	Binding_site
IPR028135	Ub_USP-typ	Domain
IPR028889	USP	Domain
IPR035927	DUSP-like_sf	Homologous_superfamily
IPR038765	Papain-like_cys_pep_sf	Homologous_superfamily
IPR050185	Ub_carboxyl-term_hydrolase	Family
IPR057368	USP32_N	Domain

Pfam: PF00443, PF06337, PF13202, PF14836, PF25265

UniProt features (37 total): binding site 9, modified residue 7, domain 5, sequence variant 4, active site 2, splice variant 2, sequence conflict 2, region of interest 2, chain 1, propeptide 1, lipid moiety-binding region 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8NFA0-F1	73.78	0.42

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (2): 743 (nucleophile); 1526 (proton acceptor)

Ligand- & substrate-binding residues (9): 241; 243; 245; 247; 252; 277; 279; 281; 288

Post-translational modifications (8): 1173, 1350, 1372, 1376, 1454, 1588, 1601, 1601

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 449 (showing top): GGGACCA_MIR133A_MIR133B, RODRIGUES_THYROID_CARCINOMA_ANAPLASTIC_UP, GOBP_REGULATION_OF_AUTOPHAGY, GOBP_RESPONSE_TO_PEPTIDE, TGCACTT_MIR519C_MIR519B_MIR519A, GOBP_CANONICAL_NF_KAPPAB_SIGNAL_TRANSDUCTION, GOBP_POSITIVE_REGULATION_OF_TOR_SIGNALING, IVANOVA_HEMATOPOIESIS_MATURE_CELL, GOBP_DNA_DAMAGE_TOLERANCE, WONG_PROTEASOME_GENE_MODULE, CACCAGC_MIR138, GOBP_REGULATION_OF_CELLULAR_COMPONENT_BIOGENESIS, GOBP_PROTEIN_MODIFICATION_BY_SMALL_PROTEIN_REMOVAL, CAGCTG_AP4_Q5, GOBP_NEGATIVE_REGULATION_OF_ORGANELLE_ASSEMBLY

GO Biological Process (3): proteolysis (GO:0006508), protein deubiquitination (GO:0016579), positive regulation of TORC1 signaling (GO:1904263)

GO Molecular Function (8): cysteine-type deubiquitinase activity (GO:0004843), calcium ion binding (GO:0005509), protein binding (GO:0005515), peptidase activity (GO:0008233), cysteine-type peptidase activity (GO:0008234), hydrolase activity (GO:0016787), metal ion binding (GO:0046872), deubiquitinase activity (GO:0101005)

GO Cellular Component (6): Golgi membrane (GO:0000139), Golgi apparatus (GO:0005794), cytosol (GO:0005829), cytoplasm (GO:0005737), endomembrane system (GO:0012505), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	4
cytoplasm	2
protein metabolic process	1
cysteine-type deubiquitinase activity	1
protein modification by small protein removal	1
positive regulation of TOR signaling	1
TORC1 signaling	1
regulation of TORC1 signaling	1
cysteine-type peptidase activity	1
deubiquitinase activity	1
metal ion binding	1
binding	1
hydrolase activity	1
catalytic activity, acting on a protein	1
peptidase activity	1
catalytic activity	1
cation binding	1
ubiquitin-like protein peptidase activity	1
Golgi apparatus	1
bounding membrane of organelle	1
endomembrane system	1
intracellular membrane-bounded organelle	1
intracellular anatomical structure	1
vacuole	1
plasma membrane	1

Protein interactions and networks

STRING

1434 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
USP32	TBC1D3	Q8IZP1	916
USP32	TBC1D3B	A6NDS4	747
USP32	APPBP2	Q92624	546
USP32	CHCT1	Q86WR6	518
USP32	USP28	Q96RU2	503
USP32	ATXN3	P54252	488
USP32	TMEM167A	Q8TBQ9	484
USP32	USP48	Q86UV5	482
USP32	BCAS3	Q9H6U6	479
USP32	USP14	P54578	475
USP32	CYLD	Q9NQC7	470
USP32	JOSD1	Q15040	465
USP32	MYSM1	Q5VVJ2	461
USP32	USP39	Q53GS9	458
USP32	USP9X	Q93008	455

IntAct

71 interactions, top by confidence:

A	B	Type	Score
N	EIF2AK2	psi-mi:“MI:0914”(association)	0.820
NIPAL1	ESYT2	psi-mi:“MI:0914”(association)	0.640
USP32	NME4	psi-mi:“MI:0914”(association)	0.560
USP32	NME4	psi-mi:“MI:0915”(physical association)	0.560
ALDH3B1	UBA6	psi-mi:“MI:0914”(association)	0.530
MANSC1	KLRG2	psi-mi:“MI:0914”(association)	0.530
FDFT1	USP32	psi-mi:“MI:0914”(association)	0.530
CD83	BTAF1	psi-mi:“MI:0914”(association)	0.530
TPCN2	AP3B1	psi-mi:“MI:0914”(association)	0.530
RAB6B	SBF1	psi-mi:“MI:0914”(association)	0.530
ENTPD7	PGK2	psi-mi:“MI:0914”(association)	0.530
SLC31A1	C2orf72	psi-mi:“MI:0914”(association)	0.530
CTLA4	B4GALT5	psi-mi:“MI:0914”(association)	0.530
KCNE3	RIOK3	psi-mi:“MI:0914”(association)	0.530
LPAR1	TMEM223	psi-mi:“MI:0914”(association)	0.530
SLC31A1	PRORP	psi-mi:“MI:0914”(association)	0.530
USP32	ZDHHC17	psi-mi:“MI:0915”(physical association)	0.510
Dlg4	USP32	psi-mi:“MI:0407”(direct interaction)	0.440
HSCB	USP32	psi-mi:“MI:0915”(physical association)	0.370
USP32	PCNA	psi-mi:“MI:0915”(physical association)	0.370
Pcna	SIVA1	psi-mi:“MI:0914”(association)	0.350
SYNCRIP	ARHGAP32	psi-mi:“MI:0914”(association)	0.350
BAG6	CNOT1	psi-mi:“MI:0914”(association)	0.350
SORT1	SH3PXD2B	psi-mi:“MI:0914”(association)	0.350
RAP1B	KLC1	psi-mi:“MI:0914”(association)	0.350
CAND1	GTPBP10	psi-mi:“MI:0914”(association)	0.350
CUL3	PXDNL	psi-mi:“MI:0914”(association)	0.350

BioGRID (155): USP32 (Two-hybrid), USP32 (Affinity Capture-Western), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), USP32 (Affinity Capture-MS), NME4 (Affinity Capture-MS)

ESM2 similar proteins: A0A0R4IB93, A1A5P5, A1Z7K9, A2XDG4, A3AF13, A3KMI0, A6QR55, B2GUZ1, D3ZJ96, F6V6I0, F6Z5C0, F8VPZ3, O22207, P29375, P35123, P51784, Q09879, Q13107, Q14149, Q2HJE4, Q30DN6, Q3UXZ9, Q3V0C5, Q5D006, Q5I043, Q5RCD3, Q5ZID5, Q5ZM45, Q62240, Q6NZP1, Q76LT8, Q80U87, Q80Y84, Q86UV5, Q8BWR4, Q8NFA0, Q8R5C8, Q8R5H1, Q93Y01, Q96RU2

Diamond homologs: A0A0R4IB93, A0JM59, A5PMR2, A5PN09, A6NNY8, A6QNM7, A7Z056, B1AY13, B1WBD7, D2HBJ8, D6RBM5, E1C213, E7F6T8, E9Q9U0, F6Z5C0, F8VPU6, F8VPZ3, M9PD06, O00507, O22207, O60079, O74442, O94269, O94966, O96612, P0C7I0, P0C8Z3, P0CAQ1, P35125, P39538, P40453, P51784, P53874, P55824, P70398, Q01988, Q09738, Q0V9G5, Q28CN3, Q2HJE4

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

186 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	164
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

5209 predictions. Top by Δscore:

Variant	Effect	Δscore
17:60179429:C:CA	acceptor_loss	1.0000
17:60179430:T:C	acceptor_loss	1.0000
17:60180549:CAG:C	donor_gain	1.0000
17:60180638:C:CC	acceptor_gain	1.0000
17:60181319:CTTA:C	donor_loss	1.0000
17:60181320:TTACC:T	donor_loss	1.0000
17:60181321:TACCG:T	donor_loss	1.0000
17:60181322:A:AC	donor_gain	1.0000
17:60181322:AC:A	donor_gain	1.0000
17:60181322:ACC:A	donor_loss	1.0000
17:60181323:C:CA	donor_gain	1.0000
17:60181323:CC:C	donor_gain	1.0000
17:60181323:CCGA:C	donor_gain	1.0000
17:60181323:CCGAA:C	donor_gain	1.0000
17:60181367:T:TA	donor_gain	1.0000
17:60181745:GAAC:G	acceptor_gain	1.0000
17:60181747:ACC:A	acceptor_loss	1.0000
17:60181749:C:CC	acceptor_gain	1.0000
17:60181749:C:CG	acceptor_loss	1.0000
17:60181750:T:A	acceptor_loss	1.0000
17:60181756:C:CT	acceptor_gain	1.0000
17:60181757:A:T	acceptor_gain	1.0000
17:60185518:CA:C	donor_gain	1.0000
17:60185557:T:TA	donor_gain	1.0000
17:60190557:ACTT:A	donor_loss	1.0000
17:60190558:CTT:C	donor_loss	1.0000
17:60190559:TTACC:T	donor_loss	1.0000
17:60190560:TACCC:T	donor_loss	1.0000
17:60190561:A:AC	donor_gain	1.0000
17:60190561:A:C	donor_loss	1.0000

AlphaMissense

10612 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
17:60180558:T:A	D1543V	1.000
17:60180558:T:C	D1543G	1.000
17:60180558:T:G	D1543A	1.000
17:60180559:C:G	D1543H	1.000
17:60180560:A:C	N1542K	1.000
17:60180560:A:T	N1542K	1.000
17:60180574:A:G	W1538R	1.000
17:60180574:A:T	W1538R	1.000
17:60180610:G:C	H1526D	1.000
17:60183392:A:T	V1299D	1.000
17:60183414:A:G	W1292R	1.000
17:60183414:A:T	W1292R	1.000
17:60183433:A:C	F1285L	1.000
17:60183433:A:T	F1285L	1.000
17:60183435:A:G	F1285L	1.000
17:60185493:C:A	K1267N	1.000
17:60185493:C:G	K1267N	1.000
17:60185518:C:G	C1259S	1.000
17:60185519:A:T	C1259S	1.000
17:60185526:A:C	C1256W	1.000
17:60185527:C:T	C1256Y	1.000
17:60185528:A:G	C1256R	1.000
17:60185578:A:G	L1239P	1.000
17:60190583:A:C	Y1208D	1.000
17:60190583:A:G	Y1208H	1.000
17:60190585:C:G	R1207P	1.000
17:60190586:G:T	R1207S	1.000
17:60190588:A:G	L1206P	1.000
17:60190588:A:T	L1206H	1.000
17:60190590:G:C	H1205Q	1.000

dbSNP variants (sampled 300 via entrez): RS1000010397 (17:60278579 G>C), RS1000013402 (17:60420196 G>T), RS1000033501 (17:60250504 G>A), RS1000042882 (17:60278203 C>T), RS1000053997 (17:60203862 A>G), RS1000065893 (17:60331487 C>G), RS1000068625 (17:60247051 C>T), RS1000093569 (17:60395391 G>C), RS1000110882 (17:60287692 G>A), RS1000112095 (17:60257401 T>C), RS1000146479 (17:60324069 C>A,T), RS1000152784 (17:60305089 G>C), RS1000157025 (17:60290899 A>T), RS1000188343 (17:60402911 G>A), RS1000205069 (17:60305562 T>A)

Disease associations

OMIM: gene MIM:607740 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST012198_7	Interleukin-6 levels	5.000000e-06

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004810	interleukin-6 measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6195760 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.67	IC50	21.2	nM	CHEMBL6195038
5.22	IC50	6027	nM	CHEMBL6195023

CTD chemical–gene interactions

29 total (human), top 29 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Lead	affects splicing, increases expression	2
Aflatoxin B1	increases methylation	2
aristolochic acid I	decreases expression	1
afimoxifene	decreases expression	1
sodium arsenite	increases expression	1
potassium chromate(VI)	affects cotreatment, decreases expression	1
coumarin	increases phosphorylation	1
cupric oxide	increases expression	1
epigallocatechin gallate	affects cotreatment, decreases expression	1
di-n-butylphosphoric acid	affects expression	1
CGP 52608	affects binding, increases reaction	1
perfluorobutanesulfonic acid	decreases expression	1
Resveratrol	affects cotreatment, increases expression	1
Fulvestrant	decreases expression	1
Acetaminophen	decreases expression	1
Arsenic	affects methylation	1
Caffeine	affects phosphorylation	1
Cisplatin	decreases expression	1
Estradiol	increases expression	1
Gallic Acid	decreases expression	1
Phenobarbital	affects expression	1
Plant Extracts	affects cotreatment, increases expression	1
Tobacco Smoke Pollution	increases expression	1
Valproic Acid	decreases methylation	1
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide	decreases expression	1
Cyclosporine	increases expression	1
Copper Sulfate	decreases expression	1
Lactic Acid	decreases expression	1
Raloxifene Hydrochloride	decreases expression	1

ChEMBL screening assays

3 unique, capped per target: 3 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL6195018	Binding	Binding affinity to human USP32 in recombinant human protein using Activity assay using Ub-RhoMP (USP32) ( Substrate: fluorogenic ubiquitin-rhodamine-morpholine (UbRhoMP))	Data for DCP probe Huib32

Cellosaurus cell lines

3 cell lines: 3 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_1661	ZR-75-30	Cancer cell line	Female
CVCL_TW88	HAP1 USP32 (-) 1	Cancer cell line	Male
CVCL_TW89	HAP1 USP32 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.