WDFY4

Summary

WDFY4 (WDFY family member 4, HGNC:29323) is a protein-coding gene on chromosome 10q11.23, encoding WD repeat- and FYVE domain-containing protein 4 (Q6ZS81). Plays a critical role in the regulation of cDC1-mediated cross-presentation of viral and tumor antigens in dendritic cells.

Predicted to be involved in antigen processing and presentation. Predicted to act upstream of or within with a positive effect on CD8-positive, alpha-beta T cell activation. Predicted to act upstream of or within cellular response to virus. Predicted to be located in early endosome and endoplasmic reticulum.

Source: NCBI Gene 57705 — RefSeq curated summary.

At a glance

• Gene–disease (curated): complex neurodevelopmental disorder (Limited, GenCC)
• GWAS associations: 21
• Clinical variants (ClinVar): 554 total
• Druggable target: yes
• MANE Select transcript: NM_001394531

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29323
Approved symbol	WDFY4
Name	WDFY family member 4
Location	10q11.23
Locus type	gene with protein product
Status	Approved
Aliases	KIAA1607, Em:AC060234.3, FLJ45748
Ensembl gene	ENSG00000128815
Ensembl biotype	protein_coding
OMIM	613316
Entrez	57705

Gene structure

Transcript identifiers

Ensembl transcripts: 7 — 4 protein_coding, 2 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000325239, ENST00000360890, ENST00000374161, ENST00000465910, ENST00000490507, ENST00000497480, ENST00000858472

RefSeq mRNA: 3 — MANE Select: NM_001394531 NM_001370154, NM_001394531, NM_020945

CCDS: CCDS44385, CCDS91241

Canonical transcript exons

ENST00000325239 — 62 exons

Exon	Start	End
ENSE00001228474	48709716	48709966
ENSE00001603847	48760347	48760440
ENSE00001614039	48788530	48788675
ENSE00001641025	48735880	48736070
ENSE00001647261	48742968	48743548
ENSE00001694351	48786639	48786870
ENSE00001715860	48778611	48778832
ENSE00001717590	48774458	48774672
ENSE00001719080	48790727	48790917
ENSE00001769365	48779941	48780119
ENSE00001780432	48776750	48776984
ENSE00001788437	48796298	48796450
ENSE00001799961	48777419	48777495
ENSE00001802112	48789874	48789985
ENSE00001804246	48775712	48775806
ENSE00003559852	48982509	48982956
ENSE00003791740	48963842	48964054
ENSE00003791805	48890579	48890727
ENSE00003792379	48877033	48877199
ENSE00003792628	48803286	48803359
ENSE00003793024	48978309	48978393
ENSE00003793143	48832573	48832709
ENSE00003793522	48807859	48807958
ENSE00003793526	48959722	48959813
ENSE00003793697	48970131	48970289
ENSE00003793779	48826671	48826909
ENSE00003793843	48974862	48975041
ENSE00003794712	48828778	48828896
ENSE00003795195	48810530	48810735
ENSE00003795900	48946040	48946157
ENSE00003795960	48875089	48875140
ENSE00003796446	48820234	48820437
ENSE00003796460	48943330	48943449
ENSE00003796561	48946860	48946969
ENSE00003796625	48805260	48805421
ENSE00003796712	48867265	48867342
ENSE00003797064	48813957	48814082
ENSE00003797149	48969064	48969248
ENSE00003797185	48806004	48806095
ENSE00003797195	48966526	48966673
ENSE00003797860	48897454	48897574
ENSE00003798148	48957129	48957282
ENSE00003798275	48981367	48981478
ENSE00003798700	48900221	48900306
ENSE00003798857	48976797	48976979
ENSE00003799284	48941806	48941848
ENSE00003799357	48873491	48873697
ENSE00003800099	48821062	48821176
ENSE00003800150	48830700	48830885
ENSE00003800241	48822380	48822537
ENSE00003800634	48901801	48901863
ENSE00003800763	48811539	48811708
ENSE00003800802	48817245	48817409
ENSE00003804697	48733931	48734035
ENSE00003805575	48729432	48729589
ENSE00003805919	48731110	48731562
ENSE00003808399	48723433	48723567
ENSE00003808751	48727470	48727659
ENSE00003809180	48721261	48721367
ENSE00003809586	48720011	48720125
ENSE00003810435	48725881	48726070
ENSE00003937228	48684873	48685001

Expression profiles

Bgee: expression breadth ubiquitous, 186 present calls, max score 91.95.

FANTOM5 (CAGE): breadth broad, TPM avg 9.2045 / max 423.3553, expressed in 487 samples.

FANTOM5 promoters (11 alternative TSS)

Promoter ID	TPM avg	Samples expressed
104859	6.3969	430
104858	1.2880	299
104860	0.8073	198
104857	0.2054	111
104861	0.1452	74
104862	0.1307	59
205862	0.0617	1
104870	0.0577	22
104863	0.0561	26
104869	0.0436	19

Top tissues by expression

238 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
superficial temporal artery	UBERON:0001614	91.95	silver quality
lymph node	UBERON:0000029	89.43	gold quality
spleen	UBERON:0002106	89.36	gold quality
granulocyte	CL:0000094	87.94	gold quality
bone marrow cell	CL:0002092	87.30	gold quality
blood	UBERON:0000178	87.25	gold quality
vermiform appendix	UBERON:0001154	85.52	gold quality
ileal mucosa	UBERON:0000331	85.06	gold quality
leukocyte	CL:0000738	84.99	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	84.89	gold quality
monocyte	CL:0000576	84.74	gold quality
sperm	CL:0000019	83.34	silver quality
trabecular bone tissue	UBERON:0002483	83.17	gold quality
bone marrow	UBERON:0002371	80.72	gold quality
caecum	UBERON:0001153	80.57	gold quality
tonsil	UBERON:0002372	80.43	gold quality
buccal mucosa cell	CL:0002336	79.22	silver quality
visceral pleura	UBERON:0002401	79.20	silver quality
epithelium of nasopharynx	UBERON:0001951	78.68	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	78.22	gold quality
upper arm skin	UBERON:0004263	77.34	gold quality
parietal pleura	UBERON:0002400	76.13	silver quality
small intestine Peyer’s patch	UBERON:0003454	75.25	gold quality
myocardium	UBERON:0002349	74.94	gold quality
palpebral conjunctiva	UBERON:0001812	74.69	gold quality
right lung	UBERON:0002167	74.69	gold quality
gall bladder	UBERON:0002110	74.48	gold quality
kidney epithelium	UBERON:0004819	74.48	gold quality
left testis	UBERON:0004533	74.46	gold quality
upper lobe of left lung	UBERON:0008952	73.96	gold quality

Single-cell (SCXA)

Detected in 7 experiment(s), a significant marker in 7.

Experiment	Marker?	Max mean expression
E-MTAB-9801	yes	2704.26
E-MTAB-6678	yes	1787.05
E-CURD-120	yes	585.02
E-HCAD-1	yes	21.55
E-MTAB-6701	yes	21.38
E-ANND-3	yes	13.98
E-MTAB-8498	yes	12.26

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): YY1

miRNA regulators (miRDB)

24 targeting WDFY4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-146A-5P	99.96	68.93	988
HSA-MIR-146B-5P	99.96	69.13	977
HSA-MIR-4666A-3P	99.96	71.71	3434
HSA-MIR-7153-5P	99.94	68.89	1006
HSA-MIR-4659A-3P	99.80	72.62	4248
HSA-MIR-4659B-3P	99.80	72.62	4248
HSA-MIR-148A-3P	99.74	73.77	1700
HSA-MIR-148B-3P	99.74	73.75	1700
HSA-MIR-152-3P	99.74	73.75	1703
HSA-MIR-7161-5P	99.68	68.92	1592
HSA-MIR-548U	99.65	67.78	1463
HSA-MIR-3182	99.40	68.15	2454
HSA-MIR-4687-5P	99.14	66.26	488
HSA-MIR-129-1-3P	98.86	68.41	779
HSA-MIR-129-2-3P	98.86	68.41	779
HSA-MIR-6829-5P	98.86	65.12	1480
HSA-MIR-4290	98.51	65.17	907
HSA-MIR-5008-5P	98.42	65.87	1019
HSA-MIR-10226	98.25	66.50	811
HSA-MIR-6779-3P	97.51	65.82	789
HSA-MIR-938	97.41	68.28	656
HSA-MIR-1587	96.95	64.03	932
HSA-MIR-3651	95.62	64.67	287

Literature-anchored findings (GeneRIF, showing 7)

• Genetic variants in ETS1 and WDFY4 were found to be associated with systemic lupus erythematosus, in the Asian population studied. (PMID:20169177)
• rs877819 SNP in WDFY4 may be associated with systemic lupus erythematosus by reduced binding of YY1 and downregulating WDFY4 expression. (PMID:22972472)
• A significantly increased risk for RA associated with the WDFY4 rs7097397 AG genotype. (PMID:24549174)
• We identified a variant in WDFY4 that was significantly associated with clinically amyopathic dermatomyositis (CADM). This variant had a cis-splicing quantitative trait locus effect for a truncated WDFY4isoform (tr-WDFY4), with higher expression in the risk allele. (PMID:29331962)
• BVVL/ FL: features caused by SLC52A3 mutations; WDFY4 and TNFSF13B may be novel causative genes. (PMID:33189404)
• Analysis of WDFY4 rs7097397 and PHLDB1 rs7389 polymorphisms in Chinese patients with systemic lupus erythematosus. (PMID:35188604)
• WDFY4 polymorphisms in Chinese patients with anti-MDA5 dermatomyositis is associated with rapid progressive interstitial lung disease. (PMID:36637178)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	wdfy4	ENSDARG00000077009
mus_musculus	Wdfy4	ENSMUSG00000051506
rattus_norvegicus	Wdfy4	ENSRNOG00000029662
caenorhabditis_elegans		WBGENE00004760
caenorhabditis_elegans		WBGENE00007752

Paralogs (7): NSMAF (ENSG00000035681), LYST (ENSG00000143669), NBEAL1 (ENSG00000144426), NBEAL2 (ENSG00000160796), WDFY3 (ENSG00000163625), NBEA (ENSG00000172915), LRBA (ENSG00000198589)

Protein

Protein identifiers

WD repeat- and FYVE domain-containing protein 4 — Q6ZS81 (reviewed: Q6ZS81)

All UniProt accessions (2): Q6ZS81, A0A1W2PQ91

UniProt curated annotations — full annotation on UniProt →

Function. Plays a critical role in the regulation of cDC1-mediated cross-presentation of viral and tumor antigens in dendritic cells. Mechanistically, acts near the plasma membrane and interacts with endosomal membranes to promote endosomal-to-cytosol antigen trafficking. Also plays a role in B-cell survival through regulation of autophagy.

Subunit / interactions. Interacts with HSP90AB1.

Subcellular location. Early endosome. Endoplasmic reticulum.

Isoforms (5)

UniProt ID	Names	Canonical?
Q6ZS81-1	1	yes
Q6ZS81-2	2
Q6ZS81-3	3
Q6ZS81-4	4
Q6ZS81-5	5

RefSeq proteins (3): NP_001357083, NP_001381460, NP_065996 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000409	BEACH_dom	Domain
IPR001680	WD40_rpt	Repeat
IPR011989	ARM-like	Homologous_superfamily
IPR011993	PH-like_dom_sf	Homologous_superfamily
IPR015943	WD40/YVTN_repeat-like_dom_sf	Homologous_superfamily
IPR016024	ARM-type_fold	Homologous_superfamily
IPR019775	WD40_repeat_CS	Conserved_site
IPR023362	PH-BEACH_dom	Domain
IPR036322	WD40_repeat_dom_sf	Homologous_superfamily
IPR036372	BEACH_dom_sf	Homologous_superfamily
IPR051944	BEACH_domain_protein	Family

Pfam: PF00400, PF02138

UniProt features (31 total): splice variant 7, repeat 6, region of interest 5, sequence conflict 4, compositionally biased region 3, sequence variant 3, domain 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

No AlphaFold model available for Q6ZS81 — AlphaFold DB does not currently provide models for proteins above ~3000 aa.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 123 (showing top): GOBP_CELLULAR_RESPONSE_TO_VIRUS, GOBP_ANTIGEN_PROCESSING_AND_PRESENTATION, TGANTCA_AP1_C, GOBP_ALPHA_BETA_T_CELL_ACTIVATION, RYTTCCTG_ETS2_B, ELK1_01, P300_01, ACEVEDO_METHYLATED_IN_LIVER_CANCER_DN, IVANOVA_HEMATOPOIESIS_EARLY_PROGENITOR, NERF_Q2, GOBP_RESPONSE_TO_VIRUS, PASQUALUCCI_LYMPHOMA_BY_GC_STAGE_DN, MGGAAGTG_GABP_B, CHEN_METABOLIC_SYNDROM_NETWORK, GOBP_LYMPHOCYTE_ACTIVATION

GO Biological Process (4): autophagy (GO:0006914), antigen processing and presentation (GO:0019882), CD8-positive, alpha-beta T cell activation (GO:0036037), cellular response to virus (GO:0098586)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (3): early endosome (GO:0005769), endoplasmic reticulum (GO:0005783), endosome (GO:0005768)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
endomembrane system	2
catabolic process	1
transmembrane transport	1
process utilizing autophagic mechanism	1
immune system process	1
alpha-beta T cell activation	1
response to virus	1
binding	1
endosome	1
cytoplasm	1
intracellular membrane-bounded organelle	1
cytoplasmic vesicle	1

Protein interactions and networks

STRING

2802 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
WDFY4	LRRC18	Q8N456	700
WDFY4	WDFY2	Q96P53	578
WDFY4	SNX22	Q96L94	544
WDFY4	WDFY1	Q8IWB7	522
WDFY4	IFIH1	Q9BYX4	490
WDFY4	BANK1	Q8NDB2	487
WDFY4	RCCD1	A6NED2	469
WDFY4	TLR9	Q9NR96	458
WDFY4	TMEM208	Q9BTX3	453
WDFY4	TLR3	O15455	441
WDFY4	BATF3	Q9NR55	432
WDFY4	LAPTM5	Q13571	430
WDFY4	MYCBP2	O75592	427
WDFY4	MPPE1	Q53F39	426
WDFY4	SEC22B	O75396	425

IntAct

5 interactions, top by confidence:

A	B	Type	Score
WDFY4	PLOD1	psi-mi:“MI:0915”(physical association)	0.400
APBB1	SSPOP	psi-mi:“MI:0914”(association)	0.350
NTRK1	ILVBL	psi-mi:“MI:0914”(association)	0.350
WDFY4	tktA	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (7): WDFY4 (Proximity Label-MS), WDFY4 (Affinity Capture-MS), HSPA8 (Cross-Linking-MS (XL-MS)), WDFY4 (Proximity Label-MS), WDFY4 (Affinity Capture-MS), WDFY4 (Affinity Capture-RNA), WDFY4 (Affinity Capture-MS)

ESM2 similar proteins: A0JMW6, A1L1L2, A1L2I9, A2A9C3, A4FV45, A8C756, B0I564, D3ZND0, E9Q2M9, G3HQ82, O15360, O35821, O70576, O75800, Q15021, Q1JPG0, Q24JP3, Q4KLN4, Q5T011, Q5U1Z0, Q5U249, Q66H56, Q6AXZ5, Q6GPP1, Q6NUQ4, Q6NXR4, Q6ZS81, Q7L4E1, Q8BGI5, Q8BK03, Q8BM55, Q8BMG7, Q8BTG3, Q8C3S2, Q8CJF7, Q8K2Z4, Q8K368, Q8WYP5, Q92574, Q99M76

Diamond homologs: A8XSV3, D4A929, E7FAW3, E9Q2M9, F4HZB2, F4IG73, F4JD14, F4JHT3, O35242, P0C6P0, P25356, P50851, P97412, Q19317, Q54PP7, Q54RQ8, Q55AV3, Q55DM1, Q562E7, Q5ND34, Q6VNB8, Q6ZNJ1, Q6ZQA0, Q6ZS30, Q6ZS81, Q7LKZ7, Q86JF2, Q8IZQ1, Q8NFP9, Q92636, Q99698, Q9DDD5, Q9EPN1, Q9ESE1, Q9TTK4, Q9W060, Q9W4E2, E7FEV0, F4I9T0, F4JY12

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

554 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	469
Likely benign	42
Benign	13

Top pathogenic / likely-pathogenic (0)

SpliceAI

10723 predictions. Top by Δscore:

Variant	Effect	Δscore
10:48709965:AGGTT:A	donor_loss	1.0000
10:48723512:T:TA	acceptor_gain	1.0000
10:48723565:CAGG:C	donor_loss	1.0000
10:48723567:GGT:G	donor_loss	1.0000
10:48723568:G:GC	donor_loss	1.0000
10:48723569:T:A	donor_loss	1.0000
10:48725879:A:AG	acceptor_gain	1.0000
10:48725879:AGAT:A	acceptor_gain	1.0000
10:48725880:G:GA	acceptor_gain	1.0000
10:48725880:G:GC	acceptor_loss	1.0000
10:48725880:GAT:G	acceptor_gain	1.0000
10:48725880:GATG:G	acceptor_gain	1.0000
10:48725880:GATGT:G	acceptor_gain	1.0000
10:48725997:G:GT	donor_gain	1.0000
10:48726060:G:GG	donor_gain	1.0000
10:48726067:CAGGG:C	donor_loss	1.0000
10:48726069:GG:G	donor_gain	1.0000
10:48726070:GG:G	donor_gain	1.0000
10:48726071:GTAT:G	donor_loss	1.0000
10:48726072:T:G	donor_loss	1.0000
10:48727468:A:AG	acceptor_gain	1.0000
10:48727469:G:GA	acceptor_gain	1.0000
10:48727658:CGGTA:C	donor_loss	1.0000
10:48727659:GGTAA:G	donor_loss	1.0000
10:48727660:G:GG	donor_gain	1.0000
10:48727660:GTAAG:G	donor_loss	1.0000
10:48727661:T:G	donor_loss	1.0000
10:48731108:AGG:A	acceptor_gain	1.0000
10:48731109:GGG:G	acceptor_gain	1.0000
10:48731534:G:GT	donor_gain	1.0000

AlphaMissense

20855 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
10:48974928:A:C	S2999R	0.998
10:48974930:C:A	S2999R	0.998
10:48974930:C:G	S2999R	0.998
10:48941843:T:A	W2542R	0.997
10:48941843:T:C	W2542R	0.997
10:48941845:G:C	W2542C	0.997
10:48941845:G:T	W2542C	0.997
10:48943423:T:A	W2575R	0.997
10:48943423:T:C	W2575R	0.997
10:48982532:G:A	G3171D	0.997
10:48982558:T:A	W3180R	0.996
10:48982558:T:C	W3180R	0.996
10:48943347:T:A	N2549K	0.995
10:48943347:T:G	N2549K	0.995
10:48959769:T:A	W2727R	0.995
10:48959769:T:C	W2727R	0.995
10:48963881:T:A	W2755R	0.995
10:48963881:T:C	W2755R	0.995
10:48981462:G:C	A3158P	0.995
10:48810567:T:A	W1626R	0.994
10:48810567:T:C	W1626R	0.994
10:48969145:C:A	A2889D	0.994
10:48976978:G:C	R3097P	0.994
10:48780027:T:A	W1162R	0.993
10:48780027:T:C	W1162R	0.993
10:48897563:T:C	F2476L	0.993
10:48897565:C:A	F2476L	0.993
10:48897565:C:G	F2476L	0.993
10:48943386:A:C	R2562S	0.993
10:48943386:A:T	R2562S	0.993

dbSNP variants (sampled 300 via entrez): RS1000023381 (10:48931463 C>T), RS1000028419 (10:48973845 C>T), RS1000028490 (10:48770882 T>C), RS1000035752 (10:48806523 C>T), RS1000042724 (10:48821573 C>T), RS1000045285 (10:48845958 C>T), RS1000053469 (10:48859178 T>G), RS1000055905 (10:48968369 C>T), RS1000061102 (10:48748621 G>A), RS1000081782 (10:48974162 G>A,T), RS1000082851 (10:48924620 A>G), RS1000114558 (10:48810543 A>C,G,T), RS1000118694 (10:48865514 G>T), RS1000131428 (10:48901176 C>T), RS1000140397 (10:48691707 A>G,T)

Disease associations

OMIM: gene MIM:613316 | disease phenotypes:

GenCC curated gene-disease

Disease	Classification	Inheritance
complex neurodevelopmental disorder	Limited	Autosomal recessive

Mondo (1): complex neurodevelopmental disorder (MONDO:0100038)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

21 associations (top):

Study	Trait	p-value
GCST000216_2	Systemic lupus erythematosus	3.000000e-06
GCST000507_14	Systemic lupus erythematosus	7.000000e-12
GCST000592_2	Systemic lupus erythematosus	8.000000e-12
GCST001308_6	Response to anti-depressant treatment in major depressive disorder	2.000000e-07
GCST001795_25	Systemic lupus erythematosus	8.000000e-09
GCST002318_166	Rheumatoid arthritis	3.000000e-09
GCST002724_18	Airway responsiveness in chronic obstructive pulmonary disease	7.000000e-06
GCST002987_21	Stroke	9.000000e-08
GCST002987_9	Stroke	9.000000e-08
GCST003155_9	Systemic lupus erythematosus	5.000000e-09
GCST003156_2	Systemic lupus erythematosus	9.000000e-12
GCST003252_27	Systemic lupus erythematosus	1.000000e-06
GCST003599_11	Systemic lupus erythematosus	2.000000e-09
GCST003622_35	Systemic lupus erythematosus	9.000000e-11
GCST003622_65	Systemic lupus erythematosus	5.000000e-07
GCST005338_1	Clinically amyopathic dermatomyositis	2.000000e-08
GCST005752_132	Systemic lupus erythematosus	7.000000e-10
GCST006959_116	Rheumatoid arthritis	6.000000e-07
GCST007400_29	Systemic lupus erythematosus	1.000000e-08
GCST007843_19	Rheumatoid arthritis	3.000000e-08
GCST011956_40	Systemic lupus erythematosus	2.000000e-40

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0006320	antidepressant-induced side effect
EFO:0006897	airway responsiveness measurement
EFO:0008532	clinically amyopathic dermatomyositis

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4742299 (PROTEIN-PROTEIN INTERACTION)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

22 total (human), top 22 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
(+)-JQ1 compound	decreases expression	2
Benzo(a)pyrene	affects methylation, decreases expression, increases methylation	2
aflatoxin B2	decreases methylation	1
di-n-butylphosphoric acid	affects expression	1
CGP 52608	affects binding, increases reaction	1
nutlin 3	affects cotreatment, increases expression	1
abrine	increases expression	1
bisphenol S	decreases methylation	1
Air Pollutants	affects expression, increases abundance	1
Dactinomycin	affects cotreatment, increases expression	1
Ethyl Methanesulfonate	decreases expression	1
Formaldehyde	decreases expression	1
Methyl Methanesulfonate	decreases expression	1
Nickel	increases expression	1
Ozone	affects expression, increases abundance	1
Phthalic Acids	increases methylation	1
Tobacco Smoke Pollution	decreases methylation	1
Triclosan	increases expression	1
Valproic Acid	increases methylation	1
Antirheumatic Agents	decreases expression	1
Cadmium Chloride	increases expression	1
Magnetite Nanoparticles	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4713759	Binding	Protac activity at CRBN/WDFY4 in human BxPC-3 cells assessed as WDFY4 degradation incubated for 16 hrs by proteomic analysis	Discovery of a Napabucasin PROTAC as an Effective Degrader of the E3 Ligase ZFP91. — J Med Chem

Clinical trials (associated diseases)

2 trials via MONDO — disease-level, not drug-specific.

Trial	Phase	Status	Title
NCT06310681	Not specified	COMPLETED	Pilot Testing of a Co-adapted Group Programme for Parents/Carers of Children With Complex Neurodisability
NCT07303049	Not specified	NOT_YET_RECRUITING	Cognitive Benefit of Intensive Rehabilitation Using Rhythmic Music Training in Children With Complex Neurodevelopmental Disorder