WDR91

Summary

WDR91 (WD repeat domain 91, HGNC:24997) is a protein-coding gene on chromosome 7q33, encoding WD repeat-containing protein 91 (A4D1P6). Functions as a negative regulator of the PI3 kinase/PI3K activity associated with endosomal membranes via BECN1, a core subunit of the PI3K complex.

Enables phosphatidylinositol 3-kinase inhibitor activity. Involved in early endosome to late endosome transport and regulation of protein catabolic process. Located in cytosol; early endosome membrane; and late endosome membrane.

Source: NCBI Gene 29062 — RefSeq curated summary.

At a glance

• Gene–disease (curated): complex neurodevelopmental disorder (Strong, GenCC)
• GWAS associations: 4
• Clinical variants (ClinVar): 80 total — 1 pathogenic, 2 likely-pathogenic
• Druggable target: yes
• MANE Select transcript: NM_014149

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24997
Approved symbol	WDR91
Name	WD repeat domain 91
Location	7q33
Locus type	gene with protein product
Status	Approved
Aliases	HSPC049, SORF-1
Ensembl gene	ENSG00000105875
Ensembl biotype	protein_coding
OMIM	616303
Entrez	29062

Gene structure

Transcript identifiers

Ensembl transcripts: 25 — 11 retained_intron, 8 protein_coding, 4 nonsense_mediated_decay, 2 protein_coding_CDS_not_defined

ENST00000354475, ENST00000423565, ENST00000462707, ENST00000466182, ENST00000474411, ENST00000476775, ENST00000479698, ENST00000485942, ENST00000497853, ENST00000682160, ENST00000682250, ENST00000682339, ENST00000682534, ENST00000682759, ENST00000682802, ENST00000683628, ENST00000683848, ENST00000683947, ENST00000684322, ENST00000684339, ENST00000684486, ENST00000956716, ENST00000956717, ENST00000956718, ENST00000956719

RefSeq mRNA: 4 — MANE Select: NM_014149 NM_001362736, NM_001362737, NM_001362738, NM_014149

CCDS: CCDS34758

Canonical transcript exons

ENST00000354475 — 15 exons

Exon	Start	End
ENSE00001806523	135183839	135186315
ENSE00003458865	135204268	135204433
ENSE00003499037	135205928	135206058
ENSE00003512649	135193578	135193672
ENSE00003519624	135197993	135198151
ENSE00003522554	135196144	135196337
ENSE00003562847	135207120	135207202
ENSE00003564829	135194934	135195084
ENSE00003598904	135186972	135187169
ENSE00003617687	135189344	135189452
ENSE00003640565	135211380	135211526
ENSE00003665157	135208791	135208998
ENSE00003668190	135209576	135209755
ENSE00003671111	135188433	135188545
ENSE00003682915	135193231	135193399

Expression profiles

Bgee: expression breadth ubiquitous, 242 present calls, max score 97.26.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 17.2114 / max 316.5239, expressed in 1809 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
86355	17.1761	1809
86352	0.0277	6
86353	0.0076	4

Top tissues by expression

275 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right uterine tube	UBERON:0001302	97.26	gold quality
metanephros cortex	UBERON:0010533	95.85	gold quality
body of pancreas	UBERON:0001150	94.23	gold quality
left ovary	UBERON:0002119	92.87	gold quality
right ovary	UBERON:0002118	92.76	gold quality
right lung	UBERON:0002167	92.71	gold quality
sural nerve	UBERON:0015488	91.79	gold quality
esophagus mucosa	UBERON:0002469	91.58	gold quality
mucosa of stomach	UBERON:0001199	91.48	gold quality
upper lobe of left lung	UBERON:0008952	90.44	gold quality
tendon of biceps brachii	UBERON:0008188	90.19	silver quality
esophagus	UBERON:0001043	90.07	gold quality
descending thoracic aorta	UBERON:0002345	89.95	gold quality
endocervix	UBERON:0000458	89.56	gold quality
adult mammalian kidney	UBERON:0000082	89.52	gold quality
right hemisphere of cerebellum	UBERON:0014890	89.48	gold quality
tibial nerve	UBERON:0001323	89.45	gold quality
ovary	UBERON:0000992	89.28	gold quality
body of stomach	UBERON:0001161	89.20	gold quality
upper lobe of lung	UBERON:0008948	89.20	gold quality
skin of abdomen	UBERON:0001416	89.17	gold quality
right coronary artery	UBERON:0001625	89.17	gold quality
skin of leg	UBERON:0001511	88.99	gold quality
ectocervix	UBERON:0012249	88.96	gold quality
lower esophagus mucosa	UBERON:0035834	88.92	gold quality
cerebellar hemisphere	UBERON:0002245	88.89	gold quality
thoracic aorta	UBERON:0001515	88.79	gold quality
lower esophagus muscularis layer	UBERON:0035833	88.79	gold quality
minor salivary gland	UBERON:0001830	88.78	gold quality
lower esophagus	UBERON:0013473	88.78	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.01
E-MTAB-7303	no	158.69

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

69 targeting WDR91, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3924	100.00	72.09	2394
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-200B-3P	100.00	73.31	2693
HSA-MIR-200C-3P	100.00	73.35	2685
HSA-MIR-429	100.00	73.44	2698
HSA-MIR-6127	100.00	66.76	2188
HSA-MIR-3692-3P	99.98	70.27	2139
HSA-MIR-1229-3P	99.97	66.49	906
HSA-MIR-2110	99.96	66.68	1930
HSA-MIR-4753-3P	99.90	71.03	3786
HSA-MIR-6739-5P	99.80	67.87	2806
HSA-MIR-6733-5P	99.74	67.94	2759
HSA-MIR-2116-3P	99.74	64.32	889
HSA-MIR-4306	99.72	70.50	3630
HSA-MIR-1208	99.70	68.28	1533
HSA-MIR-4530	99.69	66.47	1509
HSA-MIR-670-5P	99.67	69.94	1565
HSA-MIR-6516-3P	99.65	68.57	1238
HSA-MIR-6832-5P	99.58	64.82	1132
HSA-MIR-3942-3P	99.57	69.03	2854
HSA-MIR-3153	99.55	67.59	2337
HSA-MIR-4437	99.52	65.29	1266
HSA-MIR-4441	99.49	66.56	3216
HSA-MIR-143-3P	99.49	69.05	1457
HSA-MIR-4770	99.49	69.09	1451
HSA-MIR-3182	99.40	68.15	2454
HSA-MIR-185-5P	99.35	68.60	2497
HSA-MIR-4506	99.34	67.47	526
HSA-MIR-130A-5P	99.33	70.26	2623
HSA-MIR-6088	99.29	68.45	1284

Literature-anchored findings (GeneRIF, showing 2)

• suggest a role for the WDR81-WDR91 complex in the fusion of endolysosomal compartments and the absence of WDR81 leads to impaired receptor trafficking and degradation (PMID:27126989)
• WDR91 serves as a Rab7 effector that is essential for neuronal development by facilitating endosome conversion in the endosome-lysosome pathway. (PMID:28860274)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	wdr91	ENSDARG00000015153
mus_musculus	Wdr91	ENSMUSG00000058486
rattus_norvegicus	Wdr91	ENSRNOG00000010421
drosophila_melanogaster	CG6005	FBGN0038672
caenorhabditis_elegans		WBGENE00022769

Protein

Protein identifiers

WD repeat-containing protein 91 — A4D1P6 (reviewed: A4D1P6)

All UniProt accessions (6): A4D1P6, A0A804HHS8, A0A804HI90, A0A804HJP3, A0A804HKV2, C9J1X0

UniProt curated annotations — full annotation on UniProt →

Function. Functions as a negative regulator of the PI3 kinase/PI3K activity associated with endosomal membranes via BECN1, a core subunit of the PI3K complex. By modifying the phosphatidylinositol 3-phosphate/PtdInsP3 content of endosomal membranes may regulate endosome fusion, recycling, sorting and early to late endosome transport. It is for instance, required for the delivery of cargos like BST2/tetherin from early to late endosome and thereby participates indirectly to their degradation by the lysosome. May play a role in meiosis.

Subunit / interactions. Interacts with WDR81; involved in early to late endosome cargo transport. Interacts with BECN1; negatively regulates the PI3 kinase/PI3K activity associated with endosomal membranes.

Subcellular location. Early endosome membrane. Late endosome membrane.

Similarity. Belongs to the WD repeat WDR91 family.

Isoforms (3)

UniProt ID	Names	Canonical?
A4D1P6-1	1	yes
A4D1P6-2	2
A4D1P6-3	3

RefSeq proteins (4): NP_001349665, NP_001349666, NP_001349667, NP_054868* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001680	WD40_rpt	Repeat
IPR015943	WD40/YVTN_repeat-like_dom_sf	Homologous_superfamily
IPR036322	WD40_repeat_dom_sf	Homologous_superfamily
IPR039724	WDR91	Family
IPR056327	ARMC9_CTLH-like_dom	Domain

Pfam: PF00400, PF23138

UniProt features (64 total): strand 36, repeat 7, splice variant 4, sequence conflict 4, modified residue 3, turn 3, compositionally biased region 2, chain 1, sequence variant 1, helix 1, region of interest 1, coiled-coil region 1

Structure

Experimental structures (PDB)

10 structures.

PDB	Method	Resolution (Å)
8KB9	X-RAY DIFFRACTION	1.9
6VYC	X-RAY DIFFRACTION	2.1
9DTA	X-RAY DIFFRACTION	2.1
8T55	X-RAY DIFFRACTION	2.2
8SHJ	X-RAY DIFFRACTION	2.21
9EJP	X-RAY DIFFRACTION	2.22
9DTB	X-RAY DIFFRACTION	2.31
9MK7	X-RAY DIFFRACTION	2.31
9EJO	X-RAY DIFFRACTION	2.4
8KB8	X-RAY DIFFRACTION	2.49

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A4D1P6-F1	75.01	0.39

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 256, 288, 293

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-9013148	CDC42 GTPase cycle

MSigDB gene sets: 121 (showing top): GOBP_MACROMOLECULE_CATABOLIC_PROCESS, GOBP_VESICLE_MEDIATED_TRANSPORT, GOBP_NEGATIVE_REGULATION_OF_INTRACELLULAR_SIGNAL_TRANSDUCTION, GOBP_REGULATION_OF_CATABOLIC_PROCESS, GOBP_NEGATIVE_REGULATION_OF_PHOSPHATIDYLINOSITOL_3_KINASE_PROTEIN_KINASE_B_SIGNAL_TRANSDUCTION, GOBP_PHOSPHATIDYLINOSITOL_3_KINASE_PROTEIN_KINASE_B_SIGNAL_TRANSDUCTION, GOBP_REGULATION_OF_PROTEIN_CATABOLIC_PROCESS, GOBP_VESICLE_MEDIATED_TRANSPORT_BETWEEN_ENDOSOMAL_COMPARTMENTS, JAATINEN_HEMATOPOIETIC_STEM_CELL_UP, GOBP_PROTEIN_CATABOLIC_PROCESS, KONDO_PROSTATE_CANCER_HCP_WITH_H3K27ME3, GOCC_LATE_ENDOSOME_MEMBRANE, GOCC_EARLY_ENDOSOME_MEMBRANE, GOBP_PROTEOLYSIS, GOMF_ENZYME_REGULATOR_ACTIVITY

GO Biological Process (3): regulation of protein catabolic process (GO:0042176), early endosome to late endosome transport (GO:0045022), negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction (GO:0051898)

GO Molecular Function (3): phosphatidylinositol 3-kinase regulator activity (GO:0035014), phosphatidylinositol 3-kinase inhibitor activity (GO:0141039), protein binding (GO:0005515)

GO Cellular Component (8): cytosol (GO:0005829), endosome membrane (GO:0010008), early endosome membrane (GO:0031901), late endosome membrane (GO:0031902), endosome (GO:0005768), early endosome (GO:0005769), late endosome (GO:0005770), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RHO GTPase cycle	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
endosome	3
cytoplasm	2
cellular anatomical structure	2
endosome membrane	2
regulation of catabolic process	1
protein catabolic process	1
regulation of protein metabolic process	1
vesicle-mediated transport between endosomal compartments	1
phosphatidylinositol 3-kinase/protein kinase B signal transduction	1
regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	1
negative regulation of intracellular signal transduction	1
kinase regulator activity	1
phosphatidylinositol 3-kinase catalytic subunit binding	1
phosphatidylinositol 3-kinase regulator activity	1
binding	1
cytoplasmic vesicle membrane	1
bounding membrane of organelle	1
early endosome	1
late endosome	1
endomembrane system	1
cytoplasmic vesicle	1

Protein interactions and networks

STRING

610 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
WDR91	WDR81	Q562E7	916
WDR91	PIK3C3	Q8NEB9	624
WDR91	CCZ1B	P86790	593
WDR91	VPS18	Q9P253	549
WDR91	BECN1	Q14457	532
WDR91	MON1A	Q86VX9	498
WDR91	CCDC93	Q567U6	472
WDR91	RMC1	Q96DM3	447
WDR91	PRRC2B	Q5JSZ5	444
WDR91	CCDC22	O60826	439
WDR91	ASCC3	Q8N3C0	422
WDR91	MFSD12	Q6NUT3	421
WDR91	RAB7A	P51149	411
WDR91	PIKFYVE	Q9Y2I7	408
WDR91	MTMR10	Q9NXD2	401

IntAct

43 interactions, top by confidence:

A	B	Type	Score
WDR91	HTT	psi-mi:“MI:0915”(physical association)	0.670
HTT	WDR91	psi-mi:“MI:0915”(physical association)	0.670
RAB8A	WDR91	psi-mi:“MI:0914”(association)	0.600
WDR81	WDR91	psi-mi:“MI:0915”(physical association)	0.590
WDR91	WDR81	psi-mi:“MI:0915”(physical association)	0.590
NAPA	NBAS	psi-mi:“MI:0914”(association)	0.530
WDR91	bec-1	psi-mi:“MI:0915”(physical association)	0.400
NUDC	WDR91	psi-mi:“MI:0915”(physical association)	0.400
Xpo1	IFT56	psi-mi:“MI:0914”(association)	0.350
PRNP	WDR91	psi-mi:“MI:0914”(association)	0.350
LRRK2		psi-mi:“MI:0914”(association)	0.350

BioGRID (41): WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-MS), WDR91 (Affinity Capture-RNA), WDR91 (Affinity Capture-Western), WDR91 (Co-localization), WDR91 (Affinity Capture-RNA)

ESM2 similar proteins: A0A0G2KIZ8, A0A1L8GXY4, A0A571BF63, A0A8M9QN10, A2CEI4, A2RRP1, A4D1P6, A6H8T2, A9X1C6, B0FXQ5, B1WC10, B2KIQ4, B2RY71, B2RYI0, B7FF09, B7FF12, E9PYY5, F1QHZ6, Q1LXZ7, Q2HJE1, Q3UMY5, Q402B2, Q4V8G4, Q5R6T6, Q5RE88, Q5U1Z0, Q5VTH9, Q5XIZ9, Q5ZLL7, Q6DFC6, Q6DTM3, Q6GPB9, Q6P2C0, Q6TEN6, Q7TMQ7, Q7ZVR1, Q8BMG7, Q8BX17, Q8C147, Q8IWG1

Diamond homologs: A4D1P6, B2RYI0, Q05B30, Q23533, Q2HJE1, Q5R6T6, Q5ZLL7, Q6TEN6, Q7TMQ7, Q5F3D7, Q9HCU5, Q9WTV0, Q9WUQ2, Q55FJ2, Q68FJ6, B6GZD3, C4JZS6, F1DLK1, O74340, P33750, P87314, Q0D0X6, Q26458, Q4ICM0, Q4P4R3, Q5R4T8, Q5ZKU8, Q5ZLK1, Q6NVS5, Q6PAC3, Q6TNS2, Q7KWL3, Q7ZYQ6, Q803X4, Q9NV06, Q54PE0, A0CH87, A0DB19, A1CF18, A1CQI9

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

80 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	2
Uncertain significance	58
Likely benign	6
Benign	2

Top pathogenic / likely-pathogenic (3)

Variant ID	HGVS	Classification
2502091	NM_014149.4(WDR91):c.1768+1G>A	Pathogenic
2430172	NM_014149.4(WDR91):c.511+1A>G	Likely pathogenic
3064663	NM_014149.4(WDR91):c.1000C>T (p.Gln334Ter)	Likely pathogenic

SpliceAI

3226 predictions. Top by Δscore:

Variant	Effect	Δscore
7:135186311:CCCAG:C	acceptor_gain	1.0000
7:135186312:CCAG:C	acceptor_gain	1.0000
7:135186312:CCAGC:C	acceptor_gain	1.0000
7:135186313:CAG:C	acceptor_gain	1.0000
7:135186313:CAGC:C	acceptor_gain	1.0000
7:135186314:AG:A	acceptor_gain	1.0000
7:135186315:GC:G	acceptor_loss	1.0000
7:135186316:C:CC	acceptor_gain	1.0000
7:135186316:C:CG	acceptor_loss	1.0000
7:135186967:GTTAC:G	donor_loss	1.0000
7:135186968:TTAC:T	donor_loss	1.0000
7:135186969:TACC:T	donor_loss	1.0000
7:135186970:A:C	donor_loss	1.0000
7:135189342:A:AC	donor_gain	1.0000
7:135189343:C:CC	donor_gain	1.0000
7:135189448:TGGAG:T	acceptor_gain	1.0000
7:135189450:GAGC:G	acceptor_loss	1.0000
7:135189453:C:CA	acceptor_loss	1.0000
7:135189453:C:CC	acceptor_gain	1.0000
7:135189456:T:TC	acceptor_gain	1.0000
7:135193225:CCGTA:C	donor_loss	1.0000
7:135193226:CGTAC:C	donor_loss	1.0000
7:135193227:GTAC:G	donor_loss	1.0000
7:135193228:TA:T	donor_loss	1.0000
7:135193230:CCTG:C	donor_gain	1.0000
7:135193670:GAG:G	acceptor_gain	1.0000
7:135194928:ACT:A	donor_loss	1.0000
7:135194929:CTC:C	donor_loss	1.0000
7:135194930:TCA:T	donor_loss	1.0000
7:135194931:CA:C	donor_loss	1.0000

AlphaMissense

4896 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:135186203:G:T	A731D	1.000
7:135186240:A:G	W719R	1.000
7:135186240:A:T	W719R	1.000
7:135188451:G:C	S621R	1.000
7:135188451:G:T	S621R	1.000
7:135188453:T:G	S621R	1.000
7:135189385:A:G	L576P	1.000
7:135194955:C:A	W458C	1.000
7:135194955:C:G	W458C	1.000
7:135194957:A:G	W458R	1.000
7:135194957:A:T	W458R	1.000
7:135195026:A:G	W435R	1.000
7:135195026:A:T	W435R	1.000
7:135195054:G:C	S425R	1.000
7:135195054:G:T	S425R	1.000
7:135195056:T:G	S425R	1.000
7:135195058:G:T	A424D	1.000
7:135208920:A:G	W128R	1.000
7:135208920:A:T	W128R	1.000
7:135208929:A:G	W125R	1.000
7:135208929:A:T	W125R	1.000
7:135186191:C:A	G735V	0.999
7:135186191:C:T	G735D	0.999
7:135186201:A:G	S732P	0.999
7:135186204:C:G	A731P	0.999
7:135187006:A:G	L682P	0.999
7:135187160:A:G	W631R	0.999
7:135187160:A:T	W631R	0.999
7:135189352:C:G	R587P	0.999
7:135189373:C:T	G580E	0.999

dbSNP variants (sampled 300 via entrez): RS1000006460 (7:135191697 C>A,T), RS1000209767 (7:135188466 C>T), RS1000243621 (7:135208095 G>A), RS1000447724 (7:135186743 C>T), RS1000453269 (7:135196619 G>T), RS1000619778 (7:135200738 G>A,C), RS1000704804 (7:135195616 T>C), RS1000762574 (7:135183842 G>A,T), RS1000827453 (7:135200259 A>G), RS1000862461 (7:135186420 C>T), RS1000899664 (7:135184118 A>G), RS1001255177 (7:135186098 C>T), RS1001490227 (7:135207964 C>A,T), RS1001605880 (7:135200135 G>A), RS1001681545 (7:135192927 G>A,C,T)

Disease associations

OMIM: gene MIM:616303 | disease phenotypes:

GenCC curated gene-disease

Disease	Classification	Inheritance
complex neurodevelopmental disorder	Strong	Autosomal recessive

Mondo (1): complex neurodevelopmental disorder (MONDO:0100038)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST90011898_147	Alanine aminotransferase levels	2.000000e-10
GCST90011899_93	Aspartate aminotransferase levels	6.000000e-14
GCST90013663_27	Alanine aminotransferase levels	1.000000e-13
GCST90013664_78	Aspartate aminotransferase levels	7.000000e-15

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004736	aspartate aminotransferase measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL5465256 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 18 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.22	Kd	6000	nM	CHEMBL5416881
5.22	Kd	6000	nM	CHEMBL5423537
5.10	Kd	8000	nM	CHEMBL5431441
5.10	Kd	8000	nM	CHEMBL5424848

PubChem BioAssay actives

4 with measured affinity, of 31 total; 4 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
N-[3-(4-chlorophenyl)oxetan-3-yl]-4-(4-hydroxypiperidin-1-yl)benzamide	2023202: Binding affinity to C-terminal His6-tagged truncated WDR91 (unknown origin) (P392 to A747 residues) expressed in baculovirus-infected Sf9 cells assessed as dissociation constant incubated for 72 to 96 hrs by SPR analysis	kd	6.0000	uM
N-[3-(4-chlorophenyl)oxetan-3-yl]-4-(3-hydroxypyrrolidin-1-yl)benzamide	2023202: Binding affinity to C-terminal His6-tagged truncated WDR91 (unknown origin) (P392 to A747 residues) expressed in baculovirus-infected Sf9 cells assessed as dissociation constant incubated for 72 to 96 hrs by SPR analysis	kd	6.0000	uM
N-[3-(4-chlorophenyl)oxetan-3-yl]-6-methyl-5,7-dihydro-4H-thieno[2,3-c]pyridine-2-carboxamide	2023202: Binding affinity to C-terminal His6-tagged truncated WDR91 (unknown origin) (P392 to A747 residues) expressed in baculovirus-infected Sf9 cells assessed as dissociation constant incubated for 72 to 96 hrs by SPR analysis	kd	8.0000	uM
N-[3-(4-chlorophenyl)oxetan-3-yl]-5-(1H-pyrazol-5-yl)thiophene-2-carboxamide	2023202: Binding affinity to C-terminal His6-tagged truncated WDR91 (unknown origin) (P392 to A747 residues) expressed in baculovirus-infected Sf9 cells assessed as dissociation constant incubated for 72 to 96 hrs by SPR analysis	kd	8.0000	uM

CTD chemical–gene interactions

21 total (human), top 21 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression	2
3-((6-(2-methoxyphenyl)pyrimidin-4-yl)amino)phenyl)methane sulfonamide	decreases expression	1
GSK-J4	decreases expression	1
FR900359	increases phosphorylation	1
2,4,6-tribromophenol	increases expression	1
triphenyl phosphate	affects expression	1
decabromobiphenyl ether	increases expression	1
tetrabromobisphenol A	increases expression	1
di-n-butylphosphoric acid	affects expression	1
abrine	increases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
pentabrominated diphenyl ether 100	increases expression	1
hexabrominated diphenyl ether 153	increases expression	1
Benzene	increases expression	1
Cisplatin	increases expression	1
Estradiol	affects cotreatment, increases expression	1
Ivermectin	decreases expression	1
Progesterone	affects response to substance	1
Thiram	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Cadmium Chloride	decreases expression	1

ChEMBL screening assays

6 unique, capped per target: 6 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5377212	Binding	Binding affinity to C-terminal His6-tagged truncated WDR91 (unknown origin) (P392 to A747 residues) expressed in baculovirus-infected Sf9 cells assessed as dissociation constant incubated for 72 to 96 hrs by SPR analysis	Discovery of a First-in-Class Small-Molecule Ligand for WDR91 Using DNA-Encoded Chemical Library Selection Followed by Machine Learning. — J Med Chem

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_TX96	HAP1 WDR91 (-) 1	Cancer cell line	Male
CVCL_XV09	HAP1 WDR91 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

2 trials via MONDO — disease-level, not drug-specific.

Trial	Phase	Status	Title
NCT06310681	Not specified	COMPLETED	Pilot Testing of a Co-adapted Group Programme for Parents/Carers of Children With Complex Neurodisability
NCT07303049	Not specified	NOT_YET_RECRUITING	Cognitive Benefit of Intensive Rehabilitation Using Rhythmic Music Training in Children With Complex Neurodevelopmental Disorder