WIZ

Summary

WIZ (WIZ zinc finger, HGNC:30917) is a protein-coding gene on chromosome 19p13.12, encoding Protein Wiz (O95785). May link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery.

Enables several functions, including RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; histone methyltransferase binding activity; and transcription corepressor binding activity. Involved in positive regulation of nuclear cell cycle DNA replication and protein stabilization. Located in midbody and nucleoplasm. Biomarker of atherosclerosis.

Source: NCBI Gene 58525 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 148 total
• Druggable target: yes
• MANE Select transcript: NM_001371589

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30917
Approved symbol	WIZ
Name	WIZ zinc finger
Location	19p13.12
Locus type	gene with protein product
Status	Approved
Aliases	ZNF803
Ensembl gene	ENSG00000011451
Ensembl biotype	protein_coding
OMIM	619715
Entrez	58525

Gene structure

Transcript identifiers

Ensembl transcripts: 22 — 19 protein_coding, 2 retained_intron, 1 nonsense_mediated_decay

ENST00000263381, ENST00000389282, ENST00000545156, ENST00000595214, ENST00000596159, ENST00000599910, ENST00000600632, ENST00000643092, ENST00000648664, ENST00000673675, ENST00000674001, ENST00000674089, ENST00000864799, ENST00000864800, ENST00000864804, ENST00000926415, ENST00000926416, ENST00000926417, ENST00000926418, ENST00000926419, ENST00000926420, ENST00000971324

RefSeq mRNA: 5 — MANE Select: NM_001371589 NM_001330395, NM_001371589, NM_001371603, NM_001411129, NM_021241

CCDS: CCDS42516, CCDS82308, CCDS92545, CCDS92546, CCDS92547

Canonical transcript exons

ENST00000673675 — 13 exons

Exon	Start	End
ENSE00000688807	15436806	15437129
ENSE00000688835	15426982	15427533
ENSE00000688836	15425241	15425768
ENSE00000688837	15424613	15425032
ENSE00000688838	15424183	15424378
ENSE00001483118	15448103	15448367
ENSE00001505339	15429586	15430089
ENSE00002249683	15419978	15423235
ENSE00002263644	15428110	15428508
ENSE00003814464	15438578	15440715
ENSE00003820956	15442676	15442748
ENSE00003897655	15449798	15449956
ENSE00003897758	15431012	15431182

Expression profiles

Bgee: expression breadth ubiquitous, 259 present calls, max score 92.47.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 27.4376 / max 303.6705, expressed in 1814 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
179744	21.0598	1799
179745	4.0056	1613
179746	1.2099	787
179747	1.1623	766

Top tissues by expression

283 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
cortical plate	UBERON:0005343	92.47	gold quality
ganglionic eminence	UBERON:0004023	92.02	gold quality
right testis	UBERON:0004534	91.03	gold quality
left testis	UBERON:0004533	90.67	gold quality
ventricular zone	UBERON:0003053	90.39	gold quality
lower esophagus muscularis layer	UBERON:0035833	90.17	gold quality
lower esophagus	UBERON:0013473	90.15	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	89.89	gold quality
amygdala	UBERON:0001876	89.85	gold quality
apex of heart	UBERON:0002098	89.68	gold quality
right coronary artery	UBERON:0001625	89.48	gold quality
right frontal lobe	UBERON:0002810	89.36	gold quality
body of uterus	UBERON:0009853	89.13	gold quality
metanephros cortex	UBERON:0010533	88.95	gold quality
thoracic aorta	UBERON:0001515	88.90	gold quality
popliteal artery	UBERON:0002250	88.85	gold quality
tibial artery	UBERON:0007610	88.83	gold quality
aorta	UBERON:0000947	88.80	gold quality
ascending aorta	UBERON:0001496	88.80	gold quality
left coronary artery	UBERON:0001626	88.68	gold quality
descending thoracic aorta	UBERON:0002345	88.67	gold quality
muscle layer of sigmoid colon	UBERON:0035805	88.67	gold quality
left uterine tube	UBERON:0001303	88.35	gold quality
ectocervix	UBERON:0012249	88.27	gold quality
coronary artery	UBERON:0001621	88.24	gold quality
cingulate cortex	UBERON:0003027	88.17	gold quality
testis	UBERON:0000473	88.16	gold quality
right ovary	UBERON:0002118	87.97	gold quality
anterior cingulate cortex	UBERON:0009835	87.93	gold quality
endocervix	UBERON:0000458	87.79	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.41

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

124 targeting WIZ, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-6127	100.00	66.76	2188
HSA-MIR-4510	100.00	66.60	2050
HSA-MIR-6129	100.00	66.46	2080
HSA-MIR-6130	100.00	66.69	2012
HSA-MIR-6133	100.00	66.48	2064
HSA-MIR-9-5P	100.00	72.28	2361
HSA-MIR-6870-5P	99.99	68.55	2115
HSA-MIR-548AW	99.99	72.57	3559
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-4723-5P	99.97	68.70	2034
HSA-MIR-5698	99.97	68.49	2029
HSA-MIR-7111-5P	99.97	68.48	2062
HSA-MIR-1468-3P	99.96	72.74	3797
HSA-MIR-4525	99.94	64.38	675
HSA-MIR-5010-5P	99.94	64.11	705
HSA-MIR-539-5P	99.93	70.30	2855
HSA-MIR-95-5P	99.89	72.17	3973
HSA-MIR-4492	99.87	68.25	3611
HSA-MIR-4447	99.85	67.81	2900
HSA-MIR-548AZ-5P	99.83	69.94	3230
HSA-MIR-548T-5P	99.83	69.91	3220
HSA-MIR-3180-5P	99.82	69.12	2422
HSA-MIR-548AJ-5P	99.78	71.12	3085
HSA-MIR-548F-5P	99.78	71.02	3093
HSA-MIR-548G-5P	99.78	71.12	3085
HSA-MIR-548X-5P	99.78	71.12	3085
HSA-MIR-4319	99.76	69.83	2586
HSA-MIR-518A-5P	99.70	69.01	2209
HSA-MIR-527	99.70	69.01	2209

Literature-anchored findings (GeneRIF, showing 2)

• Data indicate zinc finger proteins ZNF644 and WIZ as two core subunits in the histone-lysine N-methyltransferase G9a/GLP complex, and interact with the transcription activation domain of G9a and GLP. (PMID:25789554)
• Disrupting the association of G9a-GLP with chromatin by depleting WIZ resulted in altered gene expression and protein-protein interactions that were distinguishable from that of small molecule-based inhibition of G9a/GLP (PMID:26338712)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	wiza	ENSDARG00000096428
danio_rerio	wizb	ENSDARG00000102436
mus_musculus	Wiz	ENSMUSG00000024050
rattus_norvegicus	Wiz	ENSRNOG00000006217

Paralogs (51): ZNF416 (ENSG00000083817), MYNN (ENSG00000085274), PRDM4 (ENSG00000110851), PRDM2 (ENSG00000116731), ZBTB17 (ENSG00000116809), ZNF644 (ENSG00000122482), GZF1 (ENSG00000125812), ZNF426 (ENSG00000130818), ZNF287 (ENSG00000141040), ZNF697 (ENSG00000143067), ZNF687 (ENSG00000143373), ZNF214 (ENSG00000149050), ZNF547 (ENSG00000152433), ZNF776 (ENSG00000152443), ZNF230 (ENSG00000159882), ZNF222 (ENSG00000159885), ZNF233 (ENSG00000159915), ZNF333 (ENSG00000160961), ZNF319 (ENSG00000166188), ZNF592 (ENSG00000166716), ZNF646 (ENSG00000167395), ZNF507 (ENSG00000168813), ZNF768 (ENSG00000169957), ZNF417 (ENSG00000173480), ZNF408 (ENSG00000175213), ZBTB41 (ENSG00000177888), ZNF223 (ENSG00000178386), ZNF852 (ENSG00000178917), ZNF784 (ENSG00000179922), ZNF572 (ENSG00000180938), ZNF707 (ENSG00000181135), ZNF746 (ENSG00000181220), ZNF467 (ENSG00000181444), ZNF530 (ENSG00000183647), ZNF17 (ENSG00000186272), ZNF527 (ENSG00000189164), ZKSCAN7 (ENSG00000196345), ZNF34 (ENSG00000196378), ZNF774 (ENSG00000196391), ZNF777 (ENSG00000196453)

Protein

Protein identifiers

Protein Wiz — O95785 (reviewed: O95785)

Alternative names: Widely-interspaced zinc finger-containing protein, Zinc finger protein 803

All UniProt accessions (9): O95785, A0A2R8YFV2, A0A3B3IS05, A0A669KAV7, A0A669KBG4, A0A669KBH9, B9EGQ5, M0QXA7, M0QXF8

UniProt curated annotations — full annotation on UniProt →

Function. May link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery. May be involved in EHMT1-EHMT2 heterodimer formation and stabilization.

Subunit / interactions. Interacts with EHMT1, EHMT2, CTBP1 and CTBP2. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2.

Subcellular location. Nucleus.

Domain organisation. The C2H2-type zinc finger 11 mediates interaction with EHMT1 and EHMT2.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

Isoforms (4)

UniProt ID	Names	Canonical?
O95785-1	1	yes
O95785-2	2
O95785-3	3
O95785-4	4

RefSeq proteins (5): NP_001317324, NP_001358518, NP_001358532, NP_001398058, NP_067064 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR013087	Znf_C2H2_type	Domain
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR051643	Transcr_Reg_ZincFinger	Family
IPR055125	Wiz_C_Znf	Domain

Pfam: PF23015

UniProt features (98 total): cross-link 36, modified residue 20, zinc finger region 11, region of interest 11, compositionally biased region 9, splice variant 7, chain 1, sequence conflict 1, turn 1, helix 1

Structure

Experimental structures (PDB)

3 structures.

PDB	Method	Resolution (Å)
9DJT	X-RAY DIFFRACTION	2.95
8TZX	X-RAY DIFFRACTION	3.15
9DJX	X-RAY DIFFRACTION	3.35

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O95785-F1	48.79	0.02

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (56): 996, 998, 1006, 1012, 1017, 1025, 1079, 1106, 1122, 1127, 1134, 1146, 1151, 1162, 1162, 1263, 1309, 1314, 1480, 1517 …

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 146 (showing top): MODULE_52, GOBP_POSITIVE_REGULATION_OF_DNA_REPLICATION, GOBP_REGULATION_OF_DNA_TEMPLATED_DNA_REPLICATION, GOBP_CELL_CYCLE_DNA_REPLICATION, MODULE_16, GOBP_REGULATION_OF_NUCLEAR_CELL_CYCLE_DNA_REPLICATION, MODULE_118, GOBP_POSITIVE_REGULATION_OF_DNA_TEMPLATED_DNA_REPLICATION, MODULE_239, GOBP_PROTEIN_STABILIZATION, GOBP_REGULATION_OF_CELL_CYCLE, GOBP_POSITIVE_REGULATION_OF_CELL_CYCLE_PROCESS, GOBP_REGULATION_OF_PROTEIN_STABILITY, MODULE_18, GOBP_REGULATION_OF_CELL_CYCLE_PROCESS

GO Biological Process (3): regulation of transcription by RNA polymerase II (GO:0006357), positive regulation of nuclear cell cycle DNA replication (GO:0010571), protein stabilization (GO:0050821)

GO Molecular Function (8): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), transcription corepressor binding (GO:0001222), zinc ion binding (GO:0008270), protein-containing complex binding (GO:0044877), histone methyltransferase binding (GO:1990226), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (4): nucleus (GO:0005634), nucleoplasm (GO:0005654), midbody (GO:0030496), extracellular exosome (GO:0070062)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
binding	2
cellular anatomical structure	2
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
nuclear DNA replication	1
regulation of nuclear cell cycle DNA replication	1
positive regulation of cell cycle process	1
positive regulation of DNA-templated DNA replication	1
regulation of protein stability	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
transcription coregulator binding	1
transition metal ion binding	1
enzyme binding	1
cation binding	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
extracellular vesicle	1

Protein interactions and networks

STRING

1186 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
WIZ	EHMT2	Q96KQ7	930
WIZ	CDYL	Q9Y232	773
WIZ	EHMT1	Q9H9B1	699
WIZ	ACIN1	Q9UKV3	587
WIZ	ZNF644	Q9H582	574
WIZ	CBX1	P23197	521
WIZ	PRMT5	O14744	456
WIZ	PRR14	Q9BWN1	447
WIZ	WDR77	Q9BQA1	444
WIZ	RUVBL2	Q9Y230	418
WIZ	LCOR	Q96JN0	414
WIZ	ZBTB39	O15060	402
WIZ	SETDB1	Q15047	399
WIZ	MIER2	Q8N344	382
WIZ	ANKRD12	Q6UB98	380

IntAct

175 interactions, top by confidence:

A	B	Type	Score
HDAC2	KDM1A	psi-mi:“MI:0914”(association)	0.890
CDK8	MED19	psi-mi:“MI:2364”(proximity)	0.850
HDAC1	CDK2AP1	psi-mi:“MI:0914”(association)	0.840
CTBP1	ZEB2	psi-mi:“MI:0914”(association)	0.800
HDAC1	TNRC18	psi-mi:“MI:0914”(association)	0.790
COPRS	PRMT5	psi-mi:“MI:0914”(association)	0.770
EHMT2	WIZ	psi-mi:“MI:0914”(association)	0.730
CTBP1	CBX4	psi-mi:“MI:0914”(association)	0.700
NFIC	NFIB	psi-mi:“MI:2364”(proximity)	0.690
YY1	YY2	psi-mi:“MI:0914”(association)	0.570
KSR2	POLR3A	psi-mi:“MI:0914”(association)	0.530
CTSG	WIZ	psi-mi:“MI:0914”(association)	0.530
XAGE2	WIZ	psi-mi:“MI:0914”(association)	0.530
CBX1	ZNF292	psi-mi:“MI:0914”(association)	0.530
CBX5	WIZ	psi-mi:“MI:0914”(association)	0.530
BAG2	HGS	psi-mi:“MI:0914”(association)	0.530
ZNF462	WIZ	psi-mi:“MI:0914”(association)	0.530
XAGE3	WIZ	psi-mi:“MI:0914”(association)	0.530
MIER2	WIZ	psi-mi:“MI:0914”(association)	0.530
TRIM10	WIZ	psi-mi:“MI:0914”(association)	0.530

BioGRID (287): WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), WIZ (Affinity Capture-MS), EHMT1 (Co-fractionation), WIZ (Two-hybrid), WIZ (Proximity Label-MS), CDYL (Affinity Capture-MS), EHMT2 (Affinity Capture-MS)

ESM2 similar proteins: A0JNJ4, A2APT9, A6NEL2, A6NP61, B1ASB6, B1WBS3, B2RXF5, F6WEQ6, O15015, O43918, O88282, O88286, O95785, P98168, P98169, Q2M3G4, Q2MHN3, Q2QGD7, Q3U1J1, Q3U381, Q497V6, Q5SW24, Q5SXM2, Q6YND2, Q6ZMQ8, Q6ZMY3, Q7TN08, Q7TSX9, Q80SU3, Q80YE4, Q811H0, Q8BG26, Q8BZW2, Q8C8V1, Q8IX07, Q8IY92, Q8N143, Q8N1G0, Q8NC74, Q8TBE0

Diamond homologs: O88286, O95785, Q9H582

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 201 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
RNA Polymerase III Transcription Termination	5	17.1×	1e-03
Notch-HLH transcription pathway	5	14.1×	2e-03
Deactivation of the beta-catenin transactivating complex	8	12.9×	1e-04
Gastrulation	6	10.7×	2e-03
Transcriptional Regulation by E2F6	5	10.1×	5e-03
NOTCH1 Intracellular Domain Regulates Transcription	6	9.8×	2e-03
RNA Polymerase III Abortive And Retractive Initiation	5	9.6×	6e-03
Differentiation of naive CD4+ T cells to T helper 2 cells (Th2 cells)	9	9.1×	2e-04

GO biological processes:

GO term	Partners	Fold	FDR
neuron fate specification	5	18.9×	8e-04
heterochromatin formation	12	16.5×	3e-09
negative regulation of gene expression, epigenetic	6	12.9×	9e-04
epigenetic regulation of gene expression	5	10.3×	8e-03
positive regulation of miRNA transcription	6	9.4×	4e-03
chromatin organization	12	6.4×	7e-05
chromatin remodeling	16	6.3×	2e-06
transcription by RNA polymerase II	15	5.7×	1e-05

Disease & clinical

Clinical variants and AI predictions

ClinVar

148 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	130
Likely benign	5
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2080 predictions. Top by Δscore:

Variant	Effect	Δscore
19:15423233:AACCT:A	acceptor_loss	1.0000
19:15423234:ACC:A	acceptor_loss	1.0000
19:15423235:CCTGC:C	acceptor_loss	1.0000
19:15424177:GCCTA:G	donor_loss	1.0000
19:15424178:CCTAC:C	donor_loss	1.0000
19:15424179:CTA:C	donor_loss	1.0000
19:15424180:TA:T	donor_loss	1.0000
19:15424181:A:AT	donor_loss	1.0000
19:15424182:C:A	donor_loss	1.0000
19:15424374:AAATT:A	acceptor_gain	1.0000
19:15424375:AATT:A	acceptor_gain	1.0000
19:15424376:ATT:A	acceptor_gain	1.0000
19:15424377:TT:T	acceptor_gain	1.0000
19:15424378:TCTA:T	acceptor_loss	1.0000
19:15424379:C:CC	acceptor_gain	1.0000
19:15424379:CTAA:C	acceptor_loss	1.0000
19:15424384:G:C	acceptor_gain	1.0000
19:15424384:G:GC	acceptor_gain	1.0000
19:15424609:TCA:T	donor_loss	1.0000
19:15424610:CAC:C	donor_loss	1.0000
19:15424611:A:AC	donor_gain	1.0000
19:15424612:C:CG	donor_gain	1.0000
19:15424612:CT:C	donor_gain	1.0000
19:15424612:CTG:C	donor_gain	1.0000
19:15425239:A:AC	donor_gain	1.0000
19:15425240:C:CC	donor_gain	1.0000
19:15426981:CA:C	donor_gain	1.0000
19:15427529:TGAGG:T	acceptor_gain	1.0000
19:15427530:GAGG:G	acceptor_gain	1.0000
19:15427531:AGG:A	acceptor_gain	1.0000

AlphaMissense

12137 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000062281 (19:15432525 CGGCGGCGGCGGGGGTGGG>C), RS1000067924 (19:15436356 G>T), RS1000087652 (19:15446274 T>C), RS1000169098 (19:15429185 C>A,T), RS1000205649 (19:15434919 T>C), RS1000504536 (19:15430452 G>A), RS1000596188 (19:15430750 GA>G), RS1000670034 (19:15441726 C>A), RS1000780165 (19:15436125 A>G), RS1000849247 (19:15434542 G>A), RS1000907276 (19:15420679 G>A,C), RS1000932947 (19:15447953 G>A,T), RS1000941593 (19:15436353 C>T), RS1001137551 (19:15439381 T>C), RS1001196572 (19:15436054 C>A,T)

Disease associations

OMIM: gene MIM:619715 | disease phenotypes: MIM:600057

GenCC curated gene-disease

Mondo (1): bladder exstrophy-epispadias-cloacal exstrophy complex (MONDO:0700039)

Orphanet (1): Classic bladder exstrophy (Orphanet:93930)

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST90002386_70	High light scatter reticulocyte percentage of red cells	1.000000e-10
GCST90002394_444	Monocyte percentage of white cells	3.000000e-10

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0007989	monocyte percentage of leukocytes

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL4879424 (SINGLE PROTEIN), CHEMBL6195547 (PROTEIN-PROTEIN INTERACTION)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: other protein — Zinc finger TFs

Most potent curated ligand interactions (1 total), top 1:

Ligand	Action	Affinity	Parameter
BMS-986470	Binding	14.0	pEC50

ChEMBL bioactivities

11 potent at pChembl≥5 of 11 total, top 11 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
10.08	AC50	0.083	nM	CHEMBL4862705
9.66	AC50	0.22	nM	CHEMBL4851579
9.46	AC50	0.35	nM	CHEMBL4848886
9.43	AC50	0.37	nM	CHEMBL4848069
9.33	AC50	0.47	nM	CHEMBL4862602
9.28	AC50	0.52	nM	CHEMBL4874292
7.40	EC50	40	nM	CHEMBL6091922
7.14	EC50	73	nM	CHEMBL6142736
7.11	EC50	78	nM	CHEMBL6091996
5.23	Kd	5925	nM	CHEMBL5653589
5.23	ED50	5925	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 8 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149783: Binding affinity to human WIZ incubated for 45 mins by Kinobead based pull down assay	kd	5.9249	uM

CTD chemical–gene interactions

37 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
perfluorooctane sulfonic acid	decreases expression, increases expression	2
Arsenic	affects methylation, decreases ubiquitination	2
aristolochic acid I	increases expression	1
GSK-J4	decreases expression	1
FR900359	affects phosphorylation	1
TAK-243	decreases sumoylation	1
dicrotophos	increases expression	1
bisphenol A	decreases methylation	1
trichostatin A	affects expression	1
sodium arsenite	increases expression	1
butyraldehyde	decreases expression	1
perfluorooctanoic acid	decreases expression	1
2,3-bis(3’-hydroxybenzyl)butyrolactone	affects cotreatment, increases expression	1
coumarin	increases phosphorylation	1
perfluoro-n-nonanoic acid	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
pomalidomide	decreases expression, increases degradation	1
LDN 193189	increases expression, affects cotreatment	1
Resveratrol	affects cotreatment, decreases expression	1
Vehicle Emissions	decreases expression, increases abundance	1
Benzo(a)pyrene	increases methylation	1
Cadmium	decreases expression, increases abundance	1
Caffeine	affects phosphorylation	1
Cisplatin	decreases expression	1
Coumestrol	affects cotreatment, increases expression	1
Doxorubicin	decreases expression	1
Enzyme Inhibitors	decreases activity, increases O-linked glycosylation	1
Ivermectin	decreases expression	1
Phthalic Acids	increases methylation	1
Plant Extracts	affects cotreatment, decreases expression	1

ChEMBL screening assays

15 unique, capped per target: 10 binding, 5 functional

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4828017	Binding	Induction of HiBit-tagged WIZ protein degradation expressed in HEK293T cells assessed as reduction in WIZ protein level incubated for 18 hrs by luminescence based assay	Novel Substituted Piperidine-2,6-dione Derivatives for Treating Sickle Cell Disease and β-Thalassemia. — ACS Med Chem Lett
CHEMBL6076211	Functional	In vivo molecular glue activity at CRBN/WIZ in immunocompromised naive NBSGW mouse xenografted with human CD34 positive HSPC cells assessed as induction of WIZ degradation in peripheral blood human C19-positive B cells administered orally o	Discovery and Optimization of First-in-Class Molecular Glue Degraders of the WIZ Transcription Factor for Fetal Hemoglobin Induction to Treat Sickle Cell Disease. — J Med Chem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_E2P7	HAP1 WIZ (-)	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.