XPOT

Summary

XPOT (exportin for tRNA, HGNC:12826) is a protein-coding gene on chromosome 12q14.2, encoding Exportin-T (O43592). Mediates the nuclear export of aminoacylated tRNAs. It is a selective cancer dependency (DepMap: 52.1% of cell lines).

This gene encodes a protein belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN.

Source: NCBI Gene 11260 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 97 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 52.1% of screened cell lines
• MANE Select transcript: NM_007235

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:12826
Approved symbol	XPOT
Name	exportin for tRNA
Location	12q14.2
Locus type	gene with protein product
Status	Approved
Aliases	XPO3
Ensembl gene	ENSG00000184575
Ensembl biotype	protein_coding
OMIM	603180
Entrez	11260

Gene structure

Transcript identifiers

Ensembl transcripts: 28 — 25 protein_coding, 2 retained_intron, 1 nonsense_mediated_decay

ENST00000332707, ENST00000400935, ENST00000538086, ENST00000540203, ENST00000541842, ENST00000542958, ENST00000904846, ENST00000904847, ENST00000904848, ENST00000904849, ENST00000936969, ENST00000936970, ENST00000936971, ENST00000936972, ENST00000936973, ENST00000936974, ENST00000936975, ENST00000936976, ENST00000936977, ENST00000936978, ENST00000936979, ENST00000936980, ENST00000936981, ENST00000936982, ENST00000971590, ENST00000971591, ENST00000971592, ENST00000971593

RefSeq mRNA: 1 — MANE Select: NM_007235 NM_007235

CCDS: CCDS31852

Canonical transcript exons

ENST00000332707 — 25 exons

Exon	Start	End
ENSE00001291464	64414907	64414989
ENSE00001292335	64420353	64420521
ENSE00001293584	64423005	64423043
ENSE00001294132	64433414	64433603
ENSE00001297593	64445075	64445131
ENSE00001300734	64448105	64451125
ENSE00001305262	64434794	64434909
ENSE00001306500	64439244	64439315
ENSE00001312482	64421235	64421471
ENSE00001313860	64423182	64423244
ENSE00001320499	64418046	64418115
ENSE00001325911	64425038	64425182
ENSE00001327017	64409962	64410095
ENSE00001328379	64434507	64434623
ENSE00001328493	64416698	64416754
ENSE00001329705	64435627	64435674
ENSE00001366258	64404392	64404804
ENSE00001504997	64420070	64420254
ENSE00001504998	64418876	64419094
ENSE00003507692	64424599	64424723
ENSE00003532037	64428051	64428120
ENSE00003617664	64431538	64431823
ENSE00003621476	64430049	64430287
ENSE00003651765	64425815	64425909
ENSE00003739826	64425338	64425457

Expression profiles

Bgee: expression breadth ubiquitous, 255 present calls, max score 97.01.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 59.2131 / max 390.2685, expressed in 1819 samples.

FANTOM5 promoters (9 alternative TSS)

Promoter ID	TPM avg	Samples expressed
126439	20.2709	1772
126437	16.4149	1776
126436	11.1228	1726
126435	7.5644	1688
126438	2.0612	1221
126442	1.1263	666
126440	0.2763	98
126434	0.2628	121
126441	0.1134	43

Top tissues by expression

260 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
stromal cell of endometrium	CL:0002255	97.01	gold quality
endothelial cell	CL:0000115	95.30	silver quality
endometrium epithelium	UBERON:0004811	94.46	gold quality
Brodmann (1909) area 23	UBERON:0013554	94.35	gold quality
islet of Langerhans	UBERON:0000006	94.18	gold quality
calcaneal tendon	UBERON:0003701	94.12	gold quality
bone marrow cell	CL:0002092	93.91	gold quality
ventricular zone	UBERON:0003053	93.90	gold quality
body of pancreas	UBERON:0001150	93.44	gold quality
middle temporal gyrus	UBERON:0002771	93.26	gold quality
pancreas	UBERON:0001264	92.99	gold quality
ganglionic eminence	UBERON:0004023	92.04	gold quality
smooth muscle tissue	UBERON:0001135	91.81	gold quality
vermiform appendix	UBERON:0001154	91.71	gold quality
epithelial cell of pancreas	CL:0000083	91.15	silver quality
tendon	UBERON:0000043	90.78	gold quality
adrenal tissue	UBERON:0018303	90.60	gold quality
upper lobe of left lung	UBERON:0008952	90.18	gold quality
esophagus mucosa	UBERON:0002469	89.90	gold quality
ectocervix	UBERON:0012249	89.86	gold quality
colonic epithelium	UBERON:0000397	89.83	gold quality
gingival epithelium	UBERON:0001949	89.82	gold quality
secondary oocyte	CL:0000655	89.78	gold quality
tibial nerve	UBERON:0001323	89.73	gold quality
left adrenal gland	UBERON:0001234	89.63	gold quality
left lobe of thyroid gland	UBERON:0001120	89.61	gold quality
minor salivary gland	UBERON:0001830	89.61	gold quality
primary visual cortex	UBERON:0002436	89.59	gold quality
monocyte	CL:0000576	89.51	gold quality
medial globus pallidus	UBERON:0002477	89.45	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-GEOD-84465	yes	1202.71
E-MTAB-7303	no	49.48
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

49 targeting XPOT, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-433-3P	99.98	69.37	1203
HSA-MIR-548N	99.98	71.94	4170
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-548AT-5P	99.96	70.83	2666
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753
HSA-MIR-548T-3P	99.96	70.64	3753
HSA-MIR-5680	99.91	69.83	3421
HSA-MIR-3671	99.90	73.04	3897
HSA-MIR-4753-3P	99.90	71.03	3786
HSA-MIR-3133	99.81	70.92	3506
HSA-MIR-2052	99.79	69.37	2031
HSA-MIR-1283	99.69	72.42	3009
HSA-MIR-7154-5P	99.69	70.52	1900
HSA-MIR-561-3P	99.64	70.90	3647
HSA-MIR-5197-5P	99.64	69.08	1494
HSA-MIR-510-3P	99.54	70.06	2965
HSA-MIR-513A-3P	99.39	70.63	3620
HSA-MIR-513C-3P	99.39	70.63	3620
HSA-MIR-377-3P	99.37	70.18	1905
HSA-MIR-130A-5P	99.33	70.26	2623

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 52.1% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 5)

• Results suggest that decreased expression of exportin-1 and exportin-t induced by 1alpha,25-dihydroxyvitamin D3 can be correlated to inhibition of the proliferation of HL-60 cells. (PMID:16707848)
• Exportin-t preferentially binds and exports those tRNAs that are rapidly consumed by the protein synthesis machinery. (PMID:17224653)
• Results indicated that hepatocellular carcinoma (HCC) patients with high XPOT expression level had poor prognosis. XPOT promotes tumor proliferation and invasion in HCC and may affect the tumor cell cycle through ubiquitin-mediated proteolysis. (PMID:30334580)
• Exportin-T: A Novel Prognostic Predictor and Potential Therapeutic Target for Neuroblastoma. (PMID:34469238)
• XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis Failure. (PMID:37928256)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	xpot	ENSDARG00000099652
mus_musculus	Xpot	ENSMUSG00000034667
rattus_norvegicus	Xpot	ENSRNOG00000007088
caenorhabditis_elegans		WBGENE00002080

Protein

Protein identifiers

Exportin-T — O43592 (reviewed: O43592)

Alternative names: Exportin(tRNA), tRNA exportin

All UniProt accessions (4): O43592, F5GYW6, F5GZM3, F8WDU6

UniProt curated annotations — full annotation on UniProt →

Function. Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor. XPOT then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Subunit / interactions. Found in a complex with XPOT, Ran and tRNA. Probably found in a complex with nucleoporins. Interacts with Ran and tRNA in a GTP-dependent manner.

Subcellular location. Nucleus. Cytoplasm.

Similarity. Belongs to the exportin family.

RefSeq proteins (1): NP_009166* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001494	Importin-beta_N	Domain
IPR011989	ARM-like	Homologous_superfamily
IPR013598	Exportin-1/Importin-b-like	Domain
IPR016024	ARM-type_fold	Homologous_superfamily
IPR040017	XPOT	Family
IPR045546	Exportin-T_C	Domain

Pfam: PF03810, PF08389, PF19282

UniProt features (13 total): mutagenesis site 5, region of interest 2, modified residue 2, sequence variant 2, chain 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O43592-F1	91.53	0.76

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 1, 634

Mutagenesis-validated functional residues (5):

Position	Phenotype
548–552	does not abolish binding to trna. does not abolish shuttling behavior.
550–557	abolishes binding to trna. does not abolish shuttling behavior.
405–409	abolishes binding to trna. does not abolish shuttling behavior.
539–543	does not abolish binding to trna. does not abolish shuttling behavior.
547–551	does not abolish binding to trna. does not abolish shuttling behavior.

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-6784531	tRNA processing in the nucleus

MSigDB gene sets: 240 (showing top): MORF_MTA1, BORCZUK_MALIGNANT_MESOTHELIOMA_UP, RODRIGUES_THYROID_CARCINOMA_ANAPLASTIC_UP, MORF_BUB1, GOBP_INTRACELLULAR_PROTEIN_TRANSPORT, BASSO_B_LYMPHOCYTE_NETWORK, SHAFFER_IRF4_TARGETS_IN_ACTIVATED_B_LYMPHOCYTE, MORF_HDAC2, GOMF_GTPASE_BINDING, EFC_Q6, ZHAN_MULTIPLE_MYELOMA_CD1_UP, PATIL_LIVER_CANCER, GOBP_NUCLEAR_TRANSPORT, AP1_Q4_01, LI_WILMS_TUMOR_VS_FETAL_KIDNEY_1_DN

GO Biological Process (4): tRNA export from nucleus (GO:0006409), intracellular protein transport (GO:0006886), tRNA re-export from nucleus (GO:0071528), nucleocytoplasmic transport (GO:0006913)

GO Molecular Function (4): tRNA binding (GO:0000049), small GTPase binding (GO:0031267), RNA binding (GO:0003723), protein binding (GO:0005515)

GO Cellular Component (6): nuclear pore (GO:0005643), nucleoplasm (GO:0005654), cytoplasm (GO:0005737), cytosol (GO:0005829), nuclear matrix (GO:0016363), nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
tRNA processing	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	4
nuclear lumen	2
RNA export from nucleus	1
tRNA transport	1
intracellular protein localization	1
protein transport	1
intracellular transport	1
tRNA export from nucleus	1
nuclear transport	1
RNA binding	1
GTPase binding	1
nucleic acid binding	1
binding	1
nuclear envelope	1
nuclear protein-containing complex	1
intracellular anatomical structure	1
cytoplasm	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

1990 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
XPOT	XPO4	Q9C0E2	973
XPOT	XPO1	O14980	929
XPOT	RANGAP1	P46060	871
XPOT	CSE1L	P55060	836
XPOT	RAN	P17080	831
XPOT	IPO13	O94829	783
XPOT	IPO7	O95373	767
XPOT	IPO8	O15397	753
XPOT	XPO7	Q9UIA9	744
XPOT	IPO4	Q8TEX9	727
XPOT	XPO5	Q9HAV4	704
XPOT	IPO9	Q96P70	704
XPOT	XPO6	Q96QU8	668
XPOT	NXF1	Q9UBU9	661
XPOT	TNPO2	O14787	642

IntAct

163 interactions, top by confidence:

A	B	Type	Score
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
IFT27	IFT56	psi-mi:“MI:0914”(association)	0.690
XPOT	POU6F2	psi-mi:“MI:0915”(physical association)	0.560
XPOT	RAN	psi-mi:“MI:0915”(physical association)	0.560
XPOT	KRT75	psi-mi:“MI:0915”(physical association)	0.560
XPOT	TRA2A	psi-mi:“MI:0915”(physical association)	0.560
EPHA1	EXOC5	psi-mi:“MI:0914”(association)	0.530
	XPO1	psi-mi:“MI:0914”(association)	0.530
EGFR	NDUFA4	psi-mi:“MI:0914”(association)	0.530
ARRDC4	WWP2	psi-mi:“MI:0914”(association)	0.530
CD70	METTL15	psi-mi:“MI:0914”(association)	0.530
ILVBL	SLC33A1	psi-mi:“MI:0914”(association)	0.530
EGFR	XPOT	psi-mi:“MI:0914”(association)	0.530
EBNA-LP	HAX1	psi-mi:“MI:0914”(association)	0.530
rep	NKRF	psi-mi:“MI:0914”(association)	0.500
TGOLN2	PGRMC1	psi-mi:“MI:0914”(association)	0.420
XPOT	CNPY3	psi-mi:“MI:0915”(physical association)	0.400
ERF	XPOT	psi-mi:“MI:0915”(physical association)	0.400
Tube1	RTL8C	psi-mi:“MI:0914”(association)	0.350
TENT5A	GOLGA8R	psi-mi:“MI:0914”(association)	0.350
ARMC6		psi-mi:“MI:0914”(association)	0.350
Hsph1	USP9Y	psi-mi:“MI:0914”(association)	0.350
Samm50	ZC3H18	psi-mi:“MI:0914”(association)	0.350

BioGRID (275): XPOT (Affinity Capture-MS), XPOT (Affinity Capture-MS), XPOT (Affinity Capture-MS), GCSH (Co-fractionation), NAA40 (Co-fractionation), PSMA3 (Co-fractionation), PSMA4 (Co-fractionation), WDR11 (Co-fractionation), XPOT (Co-fractionation), XPOT (Affinity Capture-MS), XPOT (Affinity Capture-MS), XPOT (Affinity Capture-MS), XPOT (Affinity Capture-MS), XPOT (Affinity Capture-MS), XPOT (Affinity Capture-MS)

ESM2 similar proteins: A0JMZ3, A7YWD2, A9UHW6, F4IRR2, F4J738, O35841, O43592, O75031, O94829, O95373, Q05AL1, Q15386, Q2KI54, Q3UBZ5, Q3UFS0, Q3ZC21, Q53I77, Q5R644, Q5R6S3, Q5R974, Q5RA02, Q5SPJ8, Q5TYQ1, Q5ZIC8, Q5ZJZ6, Q6AXU7, Q6NTZ5, Q6P2B1, Q6QI06, Q6R327, Q7Z3V4, Q80U95, Q8BHL5, Q8IUR7, Q8K0C1, Q8QHJ8, Q8R5L3, Q91W86, Q924Z6, Q96JC1

Diamond homologs: O43592, Q5RA02, Q5SPJ8, Q9CRT8

SIGNOR signaling

3 interactions.

A	Effect	B	Mechanism
RAN	“up-regulates activity”	XPOT	binding
XPOT	“up-regulates activity”	NPC	binding
NPC	“up-regulates quantity”	XPOT	relocalization

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 186 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

GO biological processes:

GO term	Partners	Fold	FDR
cell surface receptor protein tyrosine kinase signaling pathway	9	10.6×	2e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

97 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	78
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

3044 predictions. Top by Δscore:

Variant	Effect	Δscore
12:64404703:G:GT	donor_gain	1.0000
12:64409954:T:TA	acceptor_gain	1.0000
12:64409956:T:TA	acceptor_gain	1.0000
12:64409957:G:A	acceptor_gain	1.0000
12:64409957:GGCA:G	acceptor_loss	1.0000
12:64409959:CAGA:C	acceptor_loss	1.0000
12:64409960:A:AG	acceptor_gain	1.0000
12:64409960:A:C	acceptor_loss	1.0000
12:64409960:AGAT:A	acceptor_gain	1.0000
12:64409961:G:GG	acceptor_gain	1.0000
12:64409961:GA:G	acceptor_gain	1.0000
12:64409961:GAT:G	acceptor_gain	1.0000
12:64409961:GATG:G	acceptor_gain	1.0000
12:64409961:GATGT:G	acceptor_gain	1.0000
12:64410091:AAAGG:A	donor_gain	1.0000
12:64410092:AAGG:A	donor_gain	1.0000
12:64410093:AGG:A	donor_gain	1.0000
12:64410094:GG:G	donor_gain	1.0000
12:64410094:GGG:G	donor_gain	1.0000
12:64410095:GG:G	donor_gain	1.0000
12:64410096:G:GA	donor_loss	1.0000
12:64410096:G:GG	donor_gain	1.0000
12:64410097:T:G	donor_loss	1.0000
12:64414897:A:AG	acceptor_gain	1.0000
12:64414898:A:G	acceptor_gain	1.0000
12:64414904:TA:T	acceptor_loss	1.0000
12:64414905:A:AG	acceptor_gain	1.0000
12:64414905:AGGCC:A	acceptor_loss	1.0000
12:64414906:G:GC	acceptor_loss	1.0000
12:64414906:G:GG	acceptor_gain	1.0000

AlphaMissense

6358 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
12:64420096:A:C	K172N	1.000
12:64420096:A:T	K172N	1.000
12:64420107:G:T	R176M	1.000
12:64420223:T:A	W215R	1.000
12:64420223:T:C	W215R	1.000
12:64420225:G:C	W215C	1.000
12:64420225:G:T	W215C	1.000
12:64425408:G:C	R508T	1.000
12:64425409:A:C	R508S	1.000
12:64425409:A:T	R508S	1.000
12:64425836:G:C	G532R	1.000
12:64425870:G:C	R543T	1.000
12:64425870:G:T	R543M	1.000
12:64430109:G:A	G600R	1.000
12:64430109:G:C	G600R	1.000
12:64430110:G:A	G600E	1.000
12:64431545:A:C	S662R	1.000
12:64431547:T:A	S662R	1.000
12:64431547:T:G	S662R	1.000
12:64431548:A:G	K663E	1.000
12:64431549:A:T	K663I	1.000
12:64431550:A:C	K663N	1.000
12:64431550:A:T	K663N	1.000
12:64431554:T:C	F665L	1.000
12:64431556:C:A	F665L	1.000
12:64431556:C:G	F665L	1.000
12:64431686:C:G	H709D	1.000
12:64431690:G:C	R710P	1.000
12:64418915:A:G	K104E	0.999
12:64418916:A:T	K104I	0.999

dbSNP variants (sampled 300 via entrez): RS1000182374 (12:64428391 G>A), RS1000204092 (12:64417907 C>T), RS1000331263 (12:64408171 G>A), RS1000366052 (12:64434897 A>C), RS1000440734 (12:64414647 G>A), RS1000555329 (12:64415675 G>A,C), RS1000584817 (12:64423457 C>G,T), RS1000721150 (12:64409612 G>A), RS1000773987 (12:64415986 C>G), RS1000785679 (12:64408447 C>T), RS1000791628 (12:64416500 A>G), RS1000855883 (12:64415191 T>A), RS1000921285 (12:64430060 C>T), RS1000931060 (12:64410563 C>T), RS1000938102 (12:64403834 C>A,T)

Disease associations

OMIM: gene MIM:603180 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST90002390_66	Mean corpuscular hemoglobin	4.000000e-09

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004527	mean corpuscular hemoglobin

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4105803 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.53	Kd	29.87	nM	CHEMBL5653589
7.53	ED50	29.87	nM	CHEMBL5653589
5.92	Kd	1216	nM	CHEMBL3752910
5.92	ED50	1216	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 12 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149790: Binding affinity to human XPOT incubated for 45 mins by Kinobead based pull down assay	kd	0.0299	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149790: Binding affinity to human XPOT incubated for 45 mins by Kinobead based pull down assay	kd	1.2163	uM

CTD chemical–gene interactions

63 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	increases expression, affects expression, decreases expression	5
sodium arsenite	decreases expression, increases expression	5
Benzo(a)pyrene	increases methylation, decreases expression, increases expression	4
Cyclosporine	increases expression	4
Tretinoin	decreases expression	3
bisphenol F	increases expression, affects cotreatment, decreases expression	2
Acetaminophen	decreases expression, increases expression	2
Air Pollutants	decreases expression, increases abundance, increases expression	2
Calcitriol	decreases expression	2
Estradiol	increases expression	2
Tetrachlorodibenzodioxin	decreases expression	2
Tunicamycin	increases expression	2
Aflatoxin B1	affects expression, decreases expression	2
Particulate Matter	increases expression, decreases expression, increases abundance	2
aristolochic acid I	decreases expression	1
tremortin	increases expression	1
methylmercuric chloride	increases expression	1
triphenyl phosphate	affects expression	1
sodium arsenate	increases expression	1
arsenite	affects binding, decreases reaction	1
methylparaben	increases expression	1
sulforaphane	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
ochratoxin A	increases expression	1
1-nitropyrene	increases expression	1
tamibarotene	decreases expression	1
perfluorooctane sulfonic acid	increases expression	1
perfluoro-n-nonanoic acid	increases expression	1
K 7174	increases expression	1
2,2’,4,4’,5-brominated diphenyl ether	increases expression	1

ChEMBL screening assays

8 unique, capped per target: 8 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4012581	Binding	Binding affinity to XPOT protein in human INA-6 cells after 3 hrs by nanoLC-MS/MS method	Ugi Reaction-Derived α-Acyl Aminocarboxamides Bind to Phosphatidylinositol 3-Kinase-Related Kinases, Inhibit HSF1-Dependent Heat Shock Response, and Induce Apoptosis in Multiple Myeloma Cells. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.