ZBTB22

Summary

ZBTB22 (zinc finger and BTB domain containing 22, HGNC:13085) is a protein-coding gene on chromosome 6p21.32, encoding Zinc finger and BTB domain-containing protein 22 (O15209). May be involved in transcriptional regulation.

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in chromatin.

Source: NCBI Gene 9278 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 33 total
• MANE Select transcript: NM_005453

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:13085
Approved symbol	ZBTB22
Name	zinc finger and BTB domain containing 22
Location	6p21.32
Locus type	gene with protein product
Status	Approved
Aliases	BING1, ZNF297A, fruitless, fru, ZBTB22A
Ensembl gene	ENSG00000236104
Ensembl biotype	protein_coding
OMIM	611439
Entrez	9278

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 6 protein_coding

ENST00000431845, ENST00000441117, ENST00000898257, ENST00000898258, ENST00000913337, ENST00000913338

RefSeq mRNA: 2 — MANE Select: NM_005453 NM_001145338, NM_005453

CCDS: CCDS4775

Canonical transcript exons

ENST00000383196 — 0 exons

Expression profiles

Bgee: expression breadth ubiquitous, 133 present calls, max score 84.96.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 5.7179 / max 54.1666, expressed in 1743 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
73150	5.7179	1743

Top tissues by expression

134 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
apex of heart	UBERON:0002098	84.96	gold quality
granulocyte	CL:0000094	84.81	gold quality
right adrenal gland	UBERON:0001233	84.52	gold quality
right adrenal gland cortex	UBERON:0035827	84.46	gold quality
skin of abdomen	UBERON:0001416	84.17	gold quality
leukocyte	CL:0000738	84.14	gold quality
monocyte	CL:0000576	84.12	gold quality
left adrenal gland	UBERON:0001234	84.04	gold quality
cortical plate	UBERON:0005343	83.99	gold quality
lower esophagus mucosa	UBERON:0035834	83.99	gold quality
left adrenal gland cortex	UBERON:0035825	83.90	gold quality
zone of skin	UBERON:0000014	83.79	gold quality
ganglionic eminence	UBERON:0004023	83.73	gold quality
skin of leg	UBERON:0001511	83.62	gold quality
descending thoracic aorta	UBERON:0002345	83.55	gold quality
stromal cell of endometrium	CL:0002255	83.52	gold quality
ectocervix	UBERON:0012249	83.52	gold quality
popliteal artery	UBERON:0002250	83.50	gold quality
tibial artery	UBERON:0007610	83.50	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	83.42	gold quality
blood	UBERON:0000178	83.24	gold quality
right frontal lobe	UBERON:0002810	83.21	gold quality
thoracic aorta	UBERON:0001515	83.16	gold quality
ascending aorta	UBERON:0001496	83.08	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	83.04	gold quality
left uterine tube	UBERON:0001303	83.04	gold quality
islet of Langerhans	UBERON:0000006	82.94	gold quality
left lobe of thyroid gland	UBERON:0001120	82.92	gold quality
adrenal gland	UBERON:0002369	82.91	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	82.91	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-MTAB-7303	no	19.36
E-ANND-3	no	0.45

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

2 targets.

Target	Regulation
AMY2A
ZBTB22

Upstream regulators (CollecTRI, top): ZBTB22

miRNA regulators (miRDB)

61 targeting ZBTB22, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4283	100.00	66.42	2097
HSA-MIR-4476	100.00	68.18	2030
HSA-MIR-6876-5P	100.00	67.68	2126
HSA-MIR-146A-5P	99.96	68.93	988
HSA-MIR-146B-5P	99.96	69.13	977
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-4666A-3P	99.96	71.71	3434
HSA-MIR-6755-5P	99.95	65.59	464
HSA-MIR-7153-5P	99.94	68.89	1006
HSA-MIR-548J-3P	99.94	72.61	4881
HSA-MIR-381-3P	99.93	71.87	2854
HSA-MIR-548AE-3P	99.93	72.66	4867
HSA-MIR-548AH-3P	99.93	72.54	4872
HSA-MIR-548AM-3P	99.93	72.54	4872
HSA-MIR-548AQ-3P	99.93	72.66	4867
HSA-MIR-300	99.92	71.76	2856
HSA-MIR-95-5P	99.89	72.17	3973
HSA-MIR-548D-3P	99.87	70.67	4362
HSA-MIR-548BB-3P	99.86	70.58	4354
HSA-MIR-548AC	99.84	70.77	4351
HSA-MIR-548H-3P	99.84	70.80	4349
HSA-MIR-548Z	99.84	70.80	4349
HSA-MIR-1299	99.77	71.24	2389
HSA-MIR-4645-3P	99.76	69.33	993
HSA-MIR-1208	99.70	68.28	1533
HSA-MIR-7-5P	99.67	70.53	1809
HSA-MIR-6715B-5P	99.64	69.63	1420
HSA-MIR-4756-3P	99.62	66.30	1319
HSA-MIR-4269	99.55	69.89	1373

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	zbtb22b	ENSDARG00000003251
danio_rerio	zbtb22a	ENSDARG00000020176
mus_musculus	Zbtb22	ENSMUSG00000051390
rattus_norvegicus	Zbtb22	ENSRNOG00000000476

Paralogs (5): ZBTB8A (ENSG00000160062), ZBTB10 (ENSG00000205189), ZBTB9 (ENSG00000213588), C17orf113 (ENSG00000267221), ZBTB8B (ENSG00000273274)

Protein

Protein identifiers

Zinc finger and BTB domain-containing protein 22 — O15209 (reviewed: O15209)

Alternative names: Protein BING1, Zinc finger and BTB domain-containing protein 22A, Zinc finger protein 297

All UniProt accessions (3): A0A1U9X8V3, A2AB93, O15209

UniProt curated annotations — full annotation on UniProt →

Function. May be involved in transcriptional regulation.

Subcellular location. Nucleus.

Similarity. Belongs to the krueppel C2H2-type zinc-finger protein family.

RefSeq proteins (2): NP_001138810, NP_005444* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000210	BTB/POZ_dom	Domain
IPR011333	SKP1/BTB/POZ_sf	Homologous_superfamily
IPR013087	Znf_C2H2_type	Domain
IPR036236	Znf_C2H2_sf	Homologous_superfamily
IPR050457	ZnFinger_BTB_dom_contain	Family

Pfam: PF00096, PF00651

UniProt features (17 total): compositionally biased region 6, zinc finger region 3, region of interest 3, sequence variant 2, chain 1, domain 1, modified residue 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O15209-F1	52.54	0.01

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 202

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 138 (showing top): MORF_MSH3, MORF_BRCA1, MORF_ATRX, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_DN, AP2_Q3, MORF_ESR1, FOXO1_01, YY1_Q6, YY1_02, HFH8_01, HFH4_01, MORF_RAP1A, HFH1_01, SOX5_01

GO Biological Process (1): regulation of transcription by RNA polymerase II (GO:0006357)

GO Molecular Function (7): RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978), DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981), DNA binding (GO:0003677), zinc ion binding (GO:0008270), sequence-specific double-stranded DNA binding (GO:1990837), protein binding (GO:0005515), metal ion binding (GO:0046872)

GO Cellular Component (2): chromatin (GO:0000785), nucleus (GO:0005634)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
RNA polymerase II transcription regulatory region sequence-specific DNA binding	2
regulation of DNA-templated transcription	1
transcription by RNA polymerase II	1
cis-regulatory region sequence-specific DNA binding	1
chromatin	1
DNA-binding transcription factor activity	1
regulation of transcription by RNA polymerase II	1
nucleic acid binding	1
transition metal ion binding	1
double-stranded DNA binding	1
sequence-specific DNA binding	1
binding	1
cation binding	1
chromosome	1
cellular anatomical structure	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

572 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZBTB22	FBP2	O00757	952
ZBTB22	TAPBP	O15533	884
ZBTB22	FBP1	P09467	811
ZBTB22	LGALS4	P56470	662
ZBTB22	HDAC1	Q13547	516
ZBTB22	KLHL18	O94889	422
ZBTB22	PDZD2	O15018	413
ZBTB22	DAXX	Q9UER7	368
ZBTB22	HSD17B8	Q92506	363
ZBTB22	MSL2	Q9HCI7	357
ZBTB22	VPS52	Q8N1B4	353
ZBTB22	KIFC1	Q9BW19	349
ZBTB22	DSCAM	O60469	348
ZBTB22	WDR97	A6NE52	331
ZBTB22	C11orf65	Q8NCR3	322

IntAct

56 interactions, top by confidence:

A	B	Type	Score
ZBTB22	MCRS1	psi-mi:“MI:0915”(physical association)	0.720
ZBTB22	GABARAPL1	psi-mi:“MI:0915”(physical association)	0.600
	ZBTB22	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22	FXR2	psi-mi:“MI:0915”(physical association)	0.560
PIN1	ZBTB22	psi-mi:“MI:0915”(physical association)	0.560
RBM39	ZBTB22	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22	MLLT6	psi-mi:“MI:0915”(physical association)	0.560
FXR2	ZBTB22	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22	PIN1	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22		psi-mi:“MI:0915”(physical association)	0.560
MLLT6	ZBTB22	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22	SCS2	psi-mi:“MI:0915”(physical association)	0.560
EMG1	ZBTB22	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22	TCEA2	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22	MEMO1	psi-mi:“MI:0915”(physical association)	0.560
ZBTB22	VAPA	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (24): ZBTB22 (Two-hybrid), ZBTB22 (Two-hybrid), ZBTB22 (Two-hybrid), FXR2 (Two-hybrid), RBM39 (Two-hybrid), MCRS1 (Two-hybrid), ZBTB22 (Two-hybrid), ZBTB22 (Affinity Capture-MS), ZBTB22 (Two-hybrid), ZBTB22 (Two-hybrid), UBQLN4 (Two-hybrid), ZBTB22 (Two-hybrid), ZBTB22 (Two-hybrid), ZBTB22 (Two-hybrid), ZBTB22 (Two-hybrid)

ESM2 similar proteins: A0JNJ4, A4IHR5, A6H7J1, A7UKY7, D4AE48, E9Q9M8, O15209, O35615, O75081, O95785, P03966, P04198, P39881, P49796, P52746, Q01101, Q1LY51, Q29RS4, Q32KV8, Q4KLY2, Q505G8, Q5T6C5, Q5TJE2, Q61976, Q62511, Q63379, Q63ZV0, Q6AY75, Q6NUJ5, Q6NV74, Q6P0F9, Q7T3H2, Q8BG80, Q8CDC7, Q8CE64, Q8IX07, Q8N554, Q8R4U1, Q96C00, Q96JP5

Diamond homologs: A0JN76, A1L2U9, A1YEX3, A1YPR0, A2AAX3, B1WAZ8, B1WBS3, B1WBU4, B2RXF5, D3ZA50, O14867, O15062, O15156, O15209, O43167, O43298, O43829, O88282, O88939, O93567, O95365, P24278, P41182, P41183, P52739, P97302, P97303, Q08376, Q0IH98, Q0IJ29, Q0P4X6, Q0VCJ6, Q13105, Q14526, Q1H9T6, Q1L8W0, Q2T9Z7, Q3B725, Q3B7N9, Q3SWU4

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

33 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	32
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

343 predictions. Top by Δscore:

Variant	Effect	Δscore
6:33315446:C:CT	acceptor_gain	0.9900
6:33315446:C:T	acceptor_gain	0.9900
6:33316984:CT:C	acceptor_gain	0.9900
6:33316986:C:CC	acceptor_gain	0.9900
6:33317742:C:CA	donor_gain	0.9900
6:33316984:CTCTG:C	acceptor_loss	0.9800
6:33316985:TCT:T	acceptor_loss	0.9800
6:33316986:C:A	acceptor_loss	0.9800
6:33316981:GGTCT:G	acceptor_gain	0.9700
6:33317827:A:AC	donor_gain	0.9700
6:33317828:C:CC	donor_gain	0.9700
6:33316982:GTCT:G	acceptor_gain	0.9600
6:33317838:TCGGC:T	donor_gain	0.9600
6:33317839:CGGCC:C	donor_gain	0.9600
6:33317897:T:TA	donor_gain	0.9600
6:33317873:T:A	donor_gain	0.9500
6:33317781:G:GA	donor_gain	0.9300
6:33317814:G:A	donor_gain	0.9300
6:33317687:A:AC	donor_gain	0.9200
6:33317688:C:CC	donor_gain	0.9200
6:33317864:TCGCC:T	donor_gain	0.8900
6:33315442:TTCCC:T	acceptor_gain	0.8600
6:33317849:G:A	donor_gain	0.8400
6:33317706:A:AT	donor_gain	0.8200
6:33317824:A:AC	donor_gain	0.8100
6:33317845:CT:C	donor_gain	0.8100
6:33317846:TT:T	donor_gain	0.8100
6:33317847:TT:T	donor_gain	0.8100
6:33315579:C:A	donor_gain	0.7900
6:33315443:T:G	acceptor_gain	0.7800

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1003670609 (6:33318089 GC>G), RS1003885439 (6:33314867 G>A), RS1004961832 (6:33317351 C>T), RS1006445996 (6:33319369 CTTG>C), RS1007233410 (6:33318444 A>G,T), RS1008207136 (6:33317183 G>A), RS1008675216 (6:33318917 T>A,G), RS1008707649 (6:33318470 G>A), RS1009764976 (6:33314401 G>A), RS1010089014 (6:33319899 G>A), RS1010652170 (6:33318365 G>A,C), RS1011650765 (6:33316922 G>A,C), RS1012393568 (6:33317811 C>T), RS1012901442 (6:33318055 T>C,G), RS1013447009 (6:33315875 C>T)

Disease associations

OMIM: gene MIM:611439 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST004521_251	Autism spectrum disorder or schizophrenia	6.000000e-12
GCST004521_287	Autism spectrum disorder or schizophrenia	5.000000e-08
GCST011122_57	Walking pace	5.000000e-08

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

PharmGKB clinical annotations

1 annotations.

Variant	Type	Level	Drugs	Phenotypes
rs3130100	Toxicity	3	aspirin	Aspirin-induced asthma;Asthma

PharmGKB variants

1 variants.

Variant	Genes	Level	Score	#Clin annots	Drugs
rs3130100	TAPBP, ZBTB22	3	3.00	1	aspirin

CTD chemical–gene interactions

27 total (human), top 27 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	decreases expression, affects expression, affects cotreatment	4
Air Pollutants	affects cotreatment, increases abundance, increases oxidation, affects methylation	2
aristolochic acid I	decreases expression	1
FR900359	increases phosphorylation	1
triphenyl phosphate	affects expression	1
alpha-pinene	affects cotreatment, increases oxidation, increases abundance	1
methacrylaldehyde	affects cotreatment, increases oxidation, increases abundance	1
CGP 52608	affects binding, increases reaction	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
Grape Seed Proanthocyanidins	affects cotreatment, increases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
Resveratrol	affects cotreatment, decreases expression	1
Acrolein	affects cotreatment, increases oxidation, increases abundance	1
Arsenic	affects methylation	1
Atrazine	increases expression	1
Benzo(a)pyrene	affects methylation	1
Catechin	affects cotreatment, increases expression	1
Dichlorodiphenyl Dichloroethylene	decreases expression	1
Estradiol	affects expression	1
Niclosamide	increases expression	1
Ozone	affects cotreatment, increases oxidation, increases abundance	1
Plant Extracts	affects cotreatment, decreases expression	1
Tamoxifen	decreases expression	1
Urethane	decreases expression	1
Cadmium Chloride	increases expression	1
Particulate Matter	affects methylation, increases abundance	1
Volatile Organic Compounds	affects cotreatment, increases oxidation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.