ZCWPW1

Summary

ZCWPW1 (zinc finger CW-type and PWWP domain containing 1, HGNC:23486) is a protein-coding gene on chromosome 7q22.1, encoding Zinc finger CW-type PWWP domain protein 1 (Q9H0M4). Dual histone methylation reader specific for PRDM9-catalyzed histone marks (H3K4me3 and H3K36me3).

Enables histone reader activity; methyl-CpG binding activity; and methylated histone binding activity. Predicted to be involved in meiosis I; positive regulation of DNA metabolic process; and spermatogenesis. Predicted to act upstream of or within homologous chromosome pairing at meiosis. Predicted to be located in XY body. Predicted to be active in nucleus.

Source: NCBI Gene 55063 — RefSeq curated summary.

At a glance

• GWAS associations: 15
• Clinical variants (ClinVar): 111 total — 1 pathogenic
• Druggable target: yes
• MANE Select transcript: NM_001386010

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:23486
Approved symbol	ZCWPW1
Name	zinc finger CW-type and PWWP domain containing 1
Location	7q22.1
Locus type	gene with protein product
Status	Approved
Aliases	FLJ10057, DKFZp434N0510, ZCW1
Ensembl gene	ENSG00000078487
Ensembl biotype	protein_coding
OMIM	618900
Entrez	55063

Gene structure

Transcript identifiers

Ensembl transcripts: 26 — 23 protein_coding, 3 retained_intron

ENST00000360951, ENST00000398027, ENST00000464510, ENST00000471336, ENST00000472716, ENST00000479315, ENST00000490089, ENST00000490721, ENST00000684423, ENST00000857285, ENST00000857286, ENST00000857287, ENST00000857288, ENST00000857289, ENST00000857291, ENST00000857292, ENST00000857293, ENST00000857294, ENST00000857295, ENST00000857296, ENST00000857297, ENST00000857298, ENST00000857299, ENST00000958741, ENST00000958742, ENST00000958743

RefSeq mRNA: 18 — MANE Select: NM_001386010 NM_001258008, NM_001386008, NM_001386009, NM_001386010, NM_001386011, NM_001386012, NM_001386013, NM_001386015, NM_001386016, NM_001386017, NM_001386018, NM_001386019, NM_001386020, NM_001386021, NM_001386022, NM_001386023, NM_001388066, NM_017984

CCDS: CCDS43623, CCDS59067, CCDS94155

Canonical transcript exons

ENST00000684423 — 18 exons

Exon	Start	End
ENSE00001180416	100402516	100402576
ENSE00001350580	100425030	100425136
ENSE00001481569	100401889	100402041
ENSE00003469144	100409428	100409544
ENSE00003471064	100417066	100417183
ENSE00003476155	100404178	100404244
ENSE00003489214	100415975	100416097
ENSE00003538394	100403694	100403785
ENSE00003557354	100416305	100416456
ENSE00003601557	100406694	100406798
ENSE00003614669	100419111	100419189
ENSE00003617005	100405013	100405093
ENSE00003633351	100419630	100419883
ENSE00003660056	100420622	100420678
ENSE00003681962	100407228	100407303
ENSE00003684545	100408539	100408659
ENSE00003918846	100400872	100401336
ENSE00003920013	100428568	100428703

Expression profiles

Bgee: expression breadth ubiquitous, 189 present calls, max score 96.85.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 3.9216 / max 113.2223, expressed in 1398 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
85192	1.7659	960
85193	0.9887	352
85189	0.6366	268
85191	0.4733	222
85190	0.0571	14

Top tissues by expression

266 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right testis	UBERON:0004534	96.85	gold quality
left testis	UBERON:0004533	96.76	gold quality
testis	UBERON:0000473	94.87	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	92.74	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	92.70	gold quality
sural nerve	UBERON:0015488	88.74	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	86.48	gold quality
cerebellar hemisphere	UBERON:0002245	86.44	gold quality
cerebellar cortex	UBERON:0002129	86.42	gold quality
right hemisphere of cerebellum	UBERON:0014890	85.94	gold quality
cerebellum	UBERON:0002037	85.36	gold quality
spinal cord	UBERON:0002240	85.36	gold quality
calcaneal tendon	UBERON:0003701	84.53	gold quality
monocyte	CL:0000576	84.19	gold quality
mononuclear cell	CL:0000842	84.04	gold quality
apex of heart	UBERON:0002098	83.74	gold quality
leukocyte	CL:0000738	83.58	gold quality
vena cava	UBERON:0004087	83.27	gold quality
tendon	UBERON:0000043	83.20	gold quality
cerebellar vermis	UBERON:0004720	82.67	gold quality
tendon of biceps brachii	UBERON:0008188	82.09	silver quality
olfactory segment of nasal mucosa	UBERON:0005386	82.02	gold quality
right uterine tube	UBERON:0001302	81.89	gold quality
type B pancreatic cell	CL:0000169	81.31	gold quality
olfactory bulb	UBERON:0002264	81.27	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	80.96	gold quality
right lobe of liver	UBERON:0001114	80.62	gold quality
substantia nigra	UBERON:0002038	80.53	gold quality
granulocyte	CL:0000094	80.52	gold quality
inferior vagus X ganglion	UBERON:0005363	80.51	silver quality

Single-cell (SCXA)

Detected in 4 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-GEOD-124263	yes	2615.78
E-MTAB-9543	yes	1303.12
E-GEOD-134144	yes	29.07
E-ANND-3	no	4.53

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

8 targeting ZCWPW1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3154	98.94	66.55	1455
HSA-MIR-6760-5P	98.87	66.73	1515
HSA-MIR-4477A	98.83	69.75	2952
HSA-MIR-583	98.71	67.44	1791
HSA-MIR-6878-5P	98.49	67.91	2142
HSA-MIR-4662A-5P	98.48	67.18	1007
HSA-MIR-103B	95.51	66.85	441
HSA-MIR-3130-3P	94.98	66.97	574

Literature-anchored findings (GeneRIF, showing 7)

• Of those 394 variants, 34 showed strong evidence of regulatory function (RegulomeDB score <3), and only 3 of them were genome-wide significant SNPs (ZCWPW1/rs1476679, CLU/rs1532278 and ABCA7/rs3764650). (PMID:24743338)
• PILRB and GATS expression levels, within the ZCWPW1 locus, were associated with Alzheimer’s disease status. (PMID:26919393)
• rs1476679 polymorphism in ZCWPW1 is associated with late-onset Alzheimer’s disease. (PMID:26958812)
• ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair. (PMID:32744506)
• PRDM9 losses in vertebrates are coupled to those of paralogs ZCWPW1 and ZCWPW2. (PMID:35217607)
• The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots. (PMID:36068616)
• A loss-of-function variant in ZCWPW1 causes human male infertility with sperm head defect and high DNA fragmentation. (PMID:38310235)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Zcwpw1	ENSMUSG00000037108
rattus_norvegicus	Zcwpw1	ENSRNOG00000052199

Paralogs (1): ZCWPW2 (ENSG00000206559)

Protein

Protein identifiers

Zinc finger CW-type PWWP domain protein 1 — Q9H0M4 (reviewed: Q9H0M4)

All UniProt accessions (5): Q9H0M4, A0A0S2Z5N7, A0A804HK41, C9JUV7, H7C4V9

UniProt curated annotations — full annotation on UniProt →

Function. Dual histone methylation reader specific for PRDM9-catalyzed histone marks (H3K4me3 and H3K36me3). Facilitates the repair of PRDM9-induced meiotic double-strand breaks (DSBs). Essential for male fertility and spermatogenesis. Required for meiosis prophase I progression in male but not in female germ cells.

Subcellular location. Nucleus. Chromosome.

Tissue specificity. Testis.

Domain organisation. The CW-TYPE zinc finger mediates its binding to trimethylated histone H3K4me3.

Isoforms (5)

UniProt ID	Names	Canonical?
Q9H0M4-1	1	yes
Q9H0M4-2	2
Q9H0M4-3	3
Q9H0M4-4	4
Q9H0M4-5	5

RefSeq proteins (18): NP_001244937, NP_001372937, NP_001372938, NP_001372939, NP_001372940, NP_001372941, NP_001372942, NP_001372944, NP_001372945, NP_001372946, NP_001372947, NP_001372948, NP_001372949, NP_001372950, NP_001372951, NP_001372952, NP_001374995, NP_060454 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000313	PWWP_dom	Domain
IPR011124	Znf_CW	Domain
IPR042778	ZCWPW1/ZCWPW2	Family

Pfam: PF00855, PF07496

UniProt features (45 total): compositionally biased region 8, splice variant 8, mutagenesis site 7, binding site 4, strand 4, region of interest 3, sequence variant 2, turn 2, helix 2, chain 1, domain 1, modified residue 1, zinc finger region 1, coiled-coil region 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
2E61	SOLUTION NMR
2RR4	SOLUTION NMR

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H0M4-F1	56.15	0.23

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (4): 259; 264; 285; 296

Post-translational modifications (1): 636

Mutagenesis-validated functional residues (7):

Position	Phenotype
256	loss of histone h3k4me3 binding; when associated with r-301; l-302 and p-303.
300	reduced histone h3k4me3 binding but complete loss of non-methylated histone h3k4 binding.
301	loss of histone h3k4me3 binding; when associated with i-256; l-302 and p-303.
302	loss of histone h3k4me3 binding; when associated with i-256; r-301 and p-303.
303	reduced histone h3k4me3 binding but no effect on non-methylated histone h3k4 binding.
303	slightly reduced histone h3k4me3 and non-methylated histone h3k4 binding.
303	loss of histone h3k4me3 binding; when associated with i-256; r-301 and l-302.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 149 (showing top): GOBP_MEIOTIC_CHROMOSOME_SEGREGATION, GOBP_REGULATION_OF_DOUBLE_STRAND_BREAK_REPAIR, RNGTGGGC_UNKNOWN, GOBP_CHROMOSOME_ORGANIZATION, GOBP_REGULATION_OF_DNA_RECOMBINATION, TGCGCANK_UNKNOWN, E2F4DP1_01, GOBP_MALE_GAMETE_GENERATION, GGGTGGRR_PAX4_03, GOBP_REGULATION_OF_DNA_REPAIR, AACWWCAANK_UNKNOWN, E2F1DP1_01, E2F1DP2_01, GOBP_ORGANELLE_FISSION, BACH2_01

GO Biological Process (9): meiosis I (GO:0007127), homologous chromosome pairing at meiosis (GO:0007129), spermatogenesis (GO:0007283), cell differentiation (GO:0030154), epigenetic regulation of gene expression (GO:0040029), positive regulation of DNA recombination (GO:0045911), positive regulation of double-strand break repair (GO:2000781), chromatin organization (GO:0006325), double-strand break repair involved in meiotic recombination (GO:1990918)

GO Molecular Function (6): zinc ion binding (GO:0008270), histone H3K4me3 reader activity (GO:0140002), protein binding (GO:0005515), metal ion binding (GO:0046872), histone H3K36me3 reader activity (GO:0140003), histone reader activity (GO:0140566)

GO Cellular Component (3): nucleus (GO:0005634), chromosome (GO:0005694), XY body (GO:0001741)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
double-strand break repair	2
histone H3 reader activity	2
meiotic telophase I	1
meiosis I cell cycle process	1
meiotic nuclear division	1
homologous chromosome segregation	1
chromosome organization involved in meiotic cell cycle	1
developmental process involved in reproduction	1
male gamete generation	1
cellular developmental process	1
chromatin remodeling	1
regulation of gene expression	1
regulation of DNA recombination	1
DNA recombination	1
positive regulation of DNA metabolic process	1
positive regulation of DNA repair	1
regulation of double-strand break repair	1
cellular component organization	1
reciprocal meiotic recombination	1
meiotic cell cycle process	1
transition metal ion binding	1
binding	1
cation binding	1
nucleosome	1
histone binding	1
chromatin-protein adaptor activity	1
intracellular membrane-bounded organelle	1
intracellular membraneless organelle	1
sex chromosome	1
condensed chromatin of inactivated sex chromosome	1

Protein interactions and networks

STRING

1437 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ZCWPW1	SLC24A4	Q8NFF2	827
ZCWPW1	CASS4	Q9NQ75	800
ZCWPW1	NME8	Q8N427	794
ZCWPW1	FERMT2	Q96AC1	764
ZCWPW1	ABCA7	Q8IZY2	762
ZCWPW1	RIN3	Q8TB24	720
ZCWPW1	SORL1	Q92673	720
ZCWPW1	CELF1	Q92879	720
ZCWPW1	MS4A6A	Q9H2W1	719
ZCWPW1	PICALM	Q13492	718
ZCWPW1	CD2AP	Q9Y5K6	713
ZCWPW1	HLA-DRB5	Q30154	698
ZCWPW1	INPP5D	Q92835	692
ZCWPW1	BIN1	O00499	670
ZCWPW1	EPHA1	P21709	661

IntAct

8 interactions, top by confidence:

A	B	Type	Score
ZCWPW1		psi-mi:“MI:0407”(direct interaction)	0.440
ZCWPW1	H1-2	psi-mi:“MI:0915”(physical association)	0.400
ZCWPW1	PLXDC2	psi-mi:“MI:0915”(physical association)	0.400
MEF2A	REV3L	psi-mi:“MI:0914”(association)	0.350
BMI1	MEIS3P1	psi-mi:“MI:0914”(association)	0.350

BioGRID (11): ZCWPW1 (Affinity Capture-MS), ZCWPW1 (Affinity Capture-MS), MEOX2 (Two-hybrid), L3MBTL3 (Two-hybrid), VAC14 (Two-hybrid), ZCWPW1 (Proximity Label-MS), ZCWPW1 (Negative Genetic), PLXDC2 (Affinity Capture-MS), ZCWPW1 (Cross-Linking-MS (XL-MS)), ZCWPW1 (Cross-Linking-MS (XL-MS)), ZCWPW1 (Cross-Linking-MS (XL-MS))

ESM2 similar proteins: A0A1B0GUA9, A0JNH1, A1L170, A2TJV2, A6H7B4, A6NFA0, A6NGY1, A6X8Z5, A7YY35, B2RQL2, D3Z1D3, M0RD54, P43359, Q05860, Q0VF49, Q1RMX6, Q1RN00, Q2M1Z3, Q32LI3, Q3URK1, Q5EBJ4, Q5M831, Q5RJL0, Q62100, Q640N3, Q64256, Q642A3, Q66H53, Q66LM6, Q68D20, Q68DA7, Q68US1, Q6AXN6, Q6AYA8, Q6IR42, Q711Q0, Q80VY2, Q86UF4, Q8IY42, Q8TDR4

Diamond homologs: A0AHX3, A0L7L5, A2RCQ8, A2RJC8, A3DE67, A4IRL1, A4VSW4, A4VZ51, A4XK62, A6TNX0, A7Z7E7, A8MHU4, A9KR74, B1YJY5, B4U143, B5XI46, B8CX98, B8DI00, B8I1Z8, B9MK78, C0MAV6, C0MEM8, C1L2D7, C5D5Q8, E1BYJ2, O04716, O51125, O74502, O96028, P52701, P54276, P61671, Q02XM1, Q03834, Q03ES2, Q03IU4, Q045P1, Q189Q3, Q18FJ9, Q3IUH3

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

111 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	0
Uncertain significance	82
Likely benign	9
Benign	1

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
2685254	GRCh37/hg19 7q22.1(chr7:99114000-101878272)x1	Pathogenic

SpliceAI

2823 predictions. Top by Δscore:

Variant	Effect	Δscore
7:100403692:A:AC	donor_gain	1.0000
7:100403693:C:CT	donor_gain	1.0000
7:100403693:CTG:C	donor_gain	1.0000
7:100403707:T:TA	donor_gain	1.0000
7:100403710:T:TA	donor_gain	1.0000
7:100406794:TTAGA:T	acceptor_gain	1.0000
7:100406795:TAGA:T	acceptor_gain	1.0000
7:100406797:GA:G	acceptor_gain	1.0000
7:100406799:C:CC	acceptor_gain	1.0000
7:100415987:T:C	donor_gain	1.0000
7:100415991:T:A	donor_gain	1.0000
7:100416095:CTT:C	acceptor_gain	1.0000
7:100403688:T:C	donor_gain	0.9900
7:100403688:TCTTA:T	donor_loss	0.9900
7:100403689:CTTA:C	donor_loss	0.9900
7:100403690:TTACT:T	donor_loss	0.9900
7:100403691:T:TG	donor_loss	0.9900
7:100403692:ACTG:A	donor_loss	0.9900
7:100403693:C:A	donor_loss	0.9900
7:100403693:CT:C	donor_gain	0.9900
7:100403693:CTGT:C	donor_gain	0.9900
7:100403703:T:A	donor_gain	0.9900
7:100403725:T:TA	donor_gain	0.9900
7:100403783:AGT:A	acceptor_gain	0.9900
7:100403786:C:CC	acceptor_gain	0.9900
7:100403786:C:CG	acceptor_loss	0.9900
7:100405024:T:C	donor_gain	0.9900
7:100405089:TTTTT:T	acceptor_gain	0.9900
7:100406688:GCTC:G	donor_loss	0.9900
7:100406689:CT:C	donor_loss	0.9900

AlphaMissense

4278 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:100406753:A:G	W371R	0.999
7:100406753:A:T	W371R	0.999
7:100407302:A:G	W331R	0.999
7:100407302:A:T	W331R	0.999
7:100409492:C:A	R268S	0.999
7:100409492:C:G	R268S	0.999
7:100409449:A:G	W283R	0.998
7:100409449:A:T	W283R	0.998
7:100409493:C:G	R268T	0.998
7:100409497:A:G	W267R	0.998
7:100409497:A:T	W267R	0.998
7:100408564:A:G	W322R	0.997
7:100408564:A:T	W322R	0.997
7:100409495:C:A	W267C	0.997
7:100409495:C:G	W267C	0.997
7:100409521:A:G	C259R	0.997
7:100407295:C:T	G333D	0.996
7:100407300:C:A	W331C	0.996
7:100407300:C:G	W331C	0.996
7:100408560:G:T	A323D	0.996
7:100408561:C:G	A323P	0.996
7:100408573:A:G	S319P	0.996
7:100409447:C:A	W283C	0.996
7:100409447:C:G	W283C	0.996
7:100406734:A:G	L377P	0.995
7:100406751:C:A	W371C	0.995
7:100406751:C:G	W371C	0.995
7:100406758:C:G	R369P	0.995
7:100406785:A:T	V360E	0.995
7:100409443:A:G	C285R	0.995

dbSNP variants (sampled 300 via entrez): RS1000070255 (7:100402528 T>C,G), RS1000135882 (7:100413824 G>C), RS1000215 (7:100406920 T>C), RS1000217 (7:100407147 C>A,G), RS1000271968 (7:100414290 G>A), RS1000333534 (7:100428392 C>T), RS1000339558 (7:100406551 C>A,T), RS1000440512 (7:100421595 T>G), RS1000450244 (7:100421232 C>A,T), RS1000645204 (7:100427491 G>A,C,T), RS1000676116 (7:100427923 C>G), RS1000719121 (7:100415137 G>A), RS1000728752 (7:100408395 T>C), RS1000730700 (7:100407814 G>C), RS1001099584 (7:100420198 T>A)

Disease associations

OMIM: gene MIM:618900 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

15 associations (top):

Study	Trait	p-value
GCST002245_15	Alzheimer’s disease (late onset)	6.000000e-10
GCST004607_155	Plateletcrit	5.000000e-13
GCST004616_187	Platelet distribution width	5.000000e-51
GCST007319_16	Alzheimer’s disease (late onset)	6.000000e-09
GCST007319_32	Alzheimer’s disease (late onset)	2.000000e-07
GCST007320_103	Alzheimer’s disease or family history of Alzheimer’s disease	6.000000e-14
GCST007320_71	Alzheimer’s disease or family history of Alzheimer’s disease	2.000000e-15
GCST007321_32	Family history of Alzheimer’s disease	2.000000e-10
GCST007321_33	Family history of Alzheimer’s disease	1.000000e-09
GCST009021_10	Alzheimer’s disease	2.000000e-06
GCST010002_259	Refractive error	3.000000e-16
GCST010083_55	Hemoglobin levels	2.000000e-26
GCST010702_48	Subcortical volume (MOSTest)	6.000000e-10
GCST010703_289	Brain morphology (MOSTest)	6.000000e-15
GCST90002404_296	Red cell distribution width	3.000000e-14

EFO canonical traits (6, from GWAS)

EFO ID	Trait name
EFO:0007985	platelet crit
EFO:0007984	platelet component distribution width
EFO:0009268	family history of Alzheimer’s disease
EFO:0004509	hemoglobin measurement
EFO:0004346	neuroimaging measurement
EFO:0009188	Red cell distribution width

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4739863 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

25 total (human), top 25 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	decreases expression, increases methylation	2
Valproic Acid	affects expression, decreases methylation	2
Thapsigargin	increases expression	2
aristolochic acid I	increases expression	1
triphenyl phosphate	affects expression	1
beta-lapachone	increases expression	1
sodium arsenite	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
perfluorooctane sulfonic acid	increases expression	1
ICG 001	increases expression	1
jinfukang	increases expression	1
Sunitinib	increases expression	1
Air Pollutants	increases abundance, increases expression	1
Atrazine	decreases expression	1
Rotenone	increases expression	1
Selenium	affects cotreatment, increases expression	1
Silicon Dioxide	decreases expression	1
Smoke	decreases expression	1
Tobacco Smoke Pollution	affects expression	1
Tunicamycin	increases expression	1
Vitamin E	affects cotreatment, increases expression	1
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide	decreases expression	1
Cadmium Chloride	decreases expression	1
Copper Sulfate	increases expression	1
Particulate Matter	increases abundance, increases expression	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4736942	Binding	Binding affinity to ZCWPW1 PWWP1 domain (unknown origin) assessed as aggregation temperature at 400 uM by heating at 25 to 85 degC by differential static light scattering method	Discovery of Small-Molecule Antagonists of the PWWP Domain of NSD2. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.